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Pesquisa : B01.650.940.800.575.912.250.096.233 [Categoria DeCS]
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[PMID]:28480420
[Au] Autor:S X; L K; F N
[Ad] Endereço:Department of Horticultural Sciences, Cape Peninsula University of Technology, Cape Town, South Africa.
[Ti] Título:EFFECTS OF DIFFERENT HYDROPONIC SUBSTRATE COMBINATIONS AND WATERING REGIMES ON PHYSIOLOGICAL AND ANTI-FUNGAL PROPERTIES OF .
[So] Source:Afr J Tradit Complement Altern Med;14(3):89-104, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Production of medicinal plants in controlled environments, particularly hydroponic technology, provides opportunities for high quality biomass accumulation and optimizes production of secondary metabolites. Applying special watering regimes in combination with efficient soil draining is an encouraging new tool for the production of pharmaceutical relevant plants. The purpose of this paper was to evaluate the effect of substrate combinations and watering regimes on nutrient uptake, anti- activity and secondary metabolite profile of . MATERIALS AND METHODS: Coir was used as the main component for the preparation of media in different combinations; TI (Coir + vermiculite + perlite + bark), T2 (Coir + bark), T3 (Coir + perlite) and T4 (Coir + vermiculite). Plants in different treatments were grown under two watering regimes: 3 and 5-days watering intervals. At 9 weeks post treatment, plants were harvested, oven dried and tissue nutrient content, anti- activity and secondary metabolites were analyzed. RESULTS: The results showed that there were significant differences ( < 0.05) on the uptake of P, K, N, Mg, Fe, Cu, B and NH4 .The highest mean values for most nutrients were obtained in treatments under 3-days interval. Acetone extracts of under 5-days interval were the most bioactive against . The MIC values obtained are relatively lower for the rhizomes, ranging from 0.078 - 0.3125 mg/ml compared to the higher MIC values (0.375 - 0.75 mg/ml) obtained in the leaves. LC-MS analysis of acetone extracts revealed the presence of phytochemicals such as caffeic acid, quercetin, p-hydroxybenzoic acid, rutin, kaempferol, epicatechin, naringenin, hesperetin and protocatechuic acid. CONCLUSION: The antimicrobial activity and/or the phytochemical profile of the crude extracts were affected by watering regimes.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Asarum/fisiologia
Hidroponia/métodos
Extratos Vegetais/farmacologia
Água/administração & dosagem
[Mh] Termos MeSH secundário: Acetona/farmacologia
Antifúngicos/química
Asarum/química
Biomassa
Fusarium/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Compostos Fitoquímicos/química
Compostos Fitoquímicos/farmacologia
Extratos Vegetais/química
Folhas de Planta/química
Folhas de Planta/fisiologia
Rizoma/química
Rizoma/fisiologia
Água/fisiologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Phytochemicals); 0 (Plant Extracts); 059QF0KO0R (Water); 1364PS73AF (Acetone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i3.10


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[PMID]:28126415
[Au] Autor:Jing Y; Zhang YF; Shang MY; Yu J; Tang JW; Liu GX; Li YL; Li XM; Wang X; Cai SQ
[Ad] Endereço:The State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China; Tonghua Gold-horse Pharmaceutical Group Co., Ltd., Beijing 100025, China.
[Ti] Título:Phenanthrene derivatives from roots and rhizomes of Asarum heterotropoides var. mandshuricum.
[So] Source:Fitoterapia;117:101-108, 2017 Mar.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Five new phenanthrene derivatives: 9-ethoxy-7-methoxy-aristololactam IV (1), norcepharadione A N-ß-d-glucopyranoside (2), aristololactamoside I (3), aristololactamoside II (4) and aristothiolactoside (5) together with eleven known phenanthrene derivatives (6-16) were isolated from the ethanol extract of the roots and rhizomes of Asarum heterotropoides var. mandshuricum. The aristololactams with substitution of ethoxy at C-9 position (1, 9, and 10) and the sulfur-containing phenanthrene derivative (5) were reported in the genus Asarum for the first time. Furthermore, six phenanthrene glucoside derivatives (2-5, 13 and 14) were also found in this genus for the first time and compounds 7 and 9-15 were isolated from the genus Asarum for the first time. Six of them (1, 2, 9, 10, 13 and 14) were submitted to cytotoxicity test against human renal proximal tubular epithelial cell lines (HK-2) using MTT and LDH assays. Compounds 1 and 10 showed significant cytotoxic activity against HK-2 cell lines with IC values of 18.18 and 20.44µmol/L in MTT assay and 84.36 and 35.06µmol/L in LDH assay, respectively. Compound 9 showed moderate cytotoxicity in MTT assay with IC values of 95.60µmol/L, but no cytotoxicity in LDH assay. Compounds 2, 13 and 14 showed cytotoxic effect in neither MTT assay nor LDH assay. Considering the other nephrotoxic phenanthrene derivatives (6, 8, 12, 15 and 16) previously tested, the results implied the potency of renal toxicity of this herb used as a medicine.
[Mh] Termos MeSH primário: Asarum/química
Fenantrenos/química
Raízes de Plantas/química
Rizoma/química
[Mh] Termos MeSH secundário: Linhagem Celular
Células Epiteliais/efeitos dos fármacos
Seres Humanos
Túbulos Renais Proximais/citologia
Estrutura Molecular
Fenantrenos/isolamento & purificação
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenanthrenes); 0 (Plant Extracts)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE


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[PMID]:28098805
[Au] Autor:Jing Y; Zhang YF; Shang MY; Liu GX; Li YL; Wang X; Cai SQ
[Ad] Endereço:State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No. 38, Xueyuan Road, Beijing 100191, China. jing_jy@126.com.
[Ti] Título:Chemical Constituents from the Roots and Rhizomes of Asarum heterotropoides var. mandshuricum and the In Vitro Anti-Inflammatory Activity.
[So] Source:Molecules;22(1), 2017 Jan 13.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Anti-inflammatory compounds were investigated from the ethanol extract of the roots and rhizomes of var. , a traditional Chinese medicine called Xixin and used for pain and inflammatory. Nine new compounds were isolated, including six new lignans, neoasarinin A-C ( - ), neoasarininoside A and B ( and ), and asarinin B ( ), and one new monoterpene, asarincin A ( ), two new amides, asaramid II and III ( and ), and one new natural monoterpene, asaricin B ( ), along with 37 known compounds ( , - ). Their structures and absolute configurations were elucidated on the basis of spectroscopic methods and chemical analyses. This is the first report of the absolute configuration of asarinin A ( ). The 8- -4' neolignans ( - ) were reported in the genus for the first time. The 15 compounds , , - , , , , , , , - were isolated from the genus , and compounds , , , and were isolated from var. for the first time. Thirty-seven of the isolates were evaluated for anti-inflammatory activity against the release of ß-glucuronidase in polymorphonuclear leukocytes (PMNs) induced by the platelet-activating factor (PAF), and compounds , , , , , - , , , , , , , , - , , and showed potent anti-inflammatory activities in vitro, with 27.9%-72.6% inhibitions at 10 mol/L. The results of anti-inflammatory assay suggested that lignans obtained from the CHCl3 extract might be the main active components of Xixin.
[Mh] Termos MeSH primário: Amidas/química
Anti-Inflamatórios/química
Asarum/química
Medicamentos de Ervas Chinesas/química
Lignanas/química
Monoterpenos/química
[Mh] Termos MeSH secundário: 1-Alquil-2-acetilglicerofosfocolina Esterase/antagonistas & inibidores
1-Alquil-2-acetilglicerofosfocolina Esterase/farmacologia
Amidas/isolamento & purificação
Amidas/farmacologia
Animais
Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/farmacologia
Clorofórmio
Medicamentos de Ervas Chinesas/isolamento & purificação
Medicamentos de Ervas Chinesas/farmacologia
Etanol
Glucuronidase/antagonistas & inibidores
Glucuronidase/secreção
Seres Humanos
Lignanas/isolamento & purificação
Lignanas/farmacologia
Estrutura Molecular
Monoterpenos/isolamento & purificação
Monoterpenos/farmacologia
Neutrófilos/citologia
Neutrófilos/efeitos dos fármacos
Extratos Vegetais/química
Raízes de Plantas/química
Cultura Primária de Células
Ratos
Rizoma/química
Solventes/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amides); 0 (Anti-Inflammatory Agents); 0 (Drugs, Chinese Herbal); 0 (Lignans); 0 (Monoterpenes); 0 (Plant Extracts); 0 (Solvents); 3K9958V90M (Ethanol); 7V31YC746X (Chloroform); EC 3.1.1.47 (1-Alkyl-2-acetylglycerophosphocholine Esterase); EC 3.2.1.31 (Glucuronidase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE


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[PMID]:27510522
[Au] Autor:Xiang T; Yang Z; Sun B; Luo H; Zhang S; Ren B; Chen X; Zhou X; Chen Z
[Ad] Endereço:Department of Traditional Chinese Medicine, First Affiliated Hospital, Sun Yat­Sen University, Guangzhou, Guangdong 510080, P.R. China.
[Ti] Título:Traditional Chinese medicine: Pivotal role of the spleen in the metabolism of aristolochic acid I in rats is dependent on oatp2a1.
[So] Source:Mol Med Rep;14(4):3243-50, 2016 Oct.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:The genotoxicity and cytotoxicity of aristolochic acids is well documented, and the Aristolochiaceae plant family has been widely used in China and India for medical purposes. However, the mechanisms of aristolochic acid I (AAI) in treatment and toxicity remain to be fully elucidated. According to the theory of traditional Chinese medicine (TCM), the spleen is responsible for transportation and transformation, in which a substance is transformed, absorbed and distributed in the body. In the present study, rats were randomized into a blank group without spleen deficiency and a spleen deficiency group to investigate the metabolism of AAI. The results showed that the concentration of AAI was higher in the spleen deficiency group, compared with that of the blank group. To further elucidate this process, the expression of organic anion transporting peptide (oatp)2a1 in the rats of the two groups were examined following oral administration of AAI. It was observed that the mRNA level of oatp2a1 in the small intestine of the blank+AAI 60 min group was downregulated, compared with that in the blank group. Compared with the mRNA level of oatp2a1 in the spleen deficiency group, the expression levels in the lung and liver were downregulated in the spleen deficiency+AAI 5 min group, whereas expression levels in the kidney in the spleen deficiency+AAI 60 min group were upregulated. Based on the above results, it was hypothesized that the expression of oatp2a1 may be one of the mechanisms of AAI metabolism in rats. In TCM, the spleen and certain functions of the small intestine, are important in AAI metabolism, and affect the toxicity of AAI. In addition, the lung, liver and kidney may also be involved in spleen deficiency syndrome in rats.
[Mh] Termos MeSH primário: Ácidos Aristolóquicos/metabolismo
Medicamentos de Ervas Chinesas/metabolismo
Transportadores de Ânions Orgânicos/metabolismo
Baço/metabolismo
[Mh] Termos MeSH secundário: Animais
Ácidos Aristolóquicos/administração & dosagem
Ácidos Aristolóquicos/química
Ácidos Aristolóquicos/farmacocinética
Asarum/química
Medicamentos de Ervas Chinesas/administração & dosagem
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacocinética
Masculino
Medicina Tradicional Chinesa
Transportadores de Ânions Orgânicos/análise
Transportadores de Ânions Orgânicos/genética
RNA Mensageiro/genética
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aristolochic Acids); 0 (Drugs, Chinese Herbal); 0 (Organic Anion Transporters); 0 (RNA, Messenger); 94218WFP5T (aristolochic acid I)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5612


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[PMID]:27183898
[Au] Autor:Sun Q; Cao H; Zhou Y; Wang X; Jiang H; Gong L; Yang Y; Rong R
[Ad] Endereço:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
[Ti] Título:Qualitative and quantitative analysis of the chemical constituents in Mahuang-Fuzi-Xixin decoction based on high performance liquid chromatography combined with time-of-flight mass spectrometry and triple quadrupole mass spectrometers.
[So] Source:Biomed Chromatogr;30(11):1820-1834, 2016 Nov.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:High-performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF/MS) and high-performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QQQ/MS/MS) were utilized to clarify the chemical constituents of Mahuang-Fuzi-Xixin Decoction. There are 52 compounds, including alkaloids, amino acids and organic acids were identified or tentatively characterized by their characteristic high resolution mass data by HPLC-QQQ/MS/MS. In the subsequent quantitative analysis, 10 constituents, including methyl ephedrine, aconine, songrine, fuziline, neoline, talatisamine, chasmanine, benzoylmesaconine, benzoylaconine and benzoylhypaconine were simultaneously determined by HPLC-QQQ/MS/MS with multiple reaction monitoring mode. Satisfactory linearity was achieved with wide linear range and fine determination coefficient (r > 0.9992). The relative standard deviations (RSD) of inter- and intra-day precisions were <3%. This method was also validated by repeatability, stability and recovery with RSD <3% respectively. A highly sensitive and efficient method was established for chemical constituents studying, including identification and quantification of Mahuang-Fuzi-Xixin decoction.
[Mh] Termos MeSH primário: Aconitum/química
Alcaloides/análise
Asarum/química
Medicamentos de Ervas Chinesas/química
Ephedra sinica/química
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Aminoácidos/análise
Cromatografia Líquida de Alta Pressão/métodos
Limite de Detecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Alkaloids); 0 (Amino Acids); 0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160518
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.3758


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[PMID]:26928902
[Au] Autor:Fan YL; Li QJ
[Ad] Endereço:Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, the Chinese Academy of Sciences, 88# Xuefu Road, Yunnan 650223, China.
[Ti] Título:Tail-like anther crest aids pollination by manipulating pollinator's behaviour in a wild ginger.
[So] Source:Sci Rep;6:22340, 2016 Mar 01.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Innovative floral organs are widely distributed taxonomically in angiosperms, and some of them are conspicuous and curious in morphology. Floral organs have long been supposed to play a crucial role in fertilization by pollinators. However, why innovative organs occur, how they are adapted for pollinators and what sexual roles they play are still puzzling. Here we focused on a wild ginger (Zingiber densissimum, Zingiberaceae) and tested the function of the curious anther crest, an innovative floral structure widely distributed in Zingiberaceae. The anther crest is a specialized anther appendage that extends up from the top of the anther to form a tail-like structure, about 150% as long as the anther. We found this structure promoted both the male and the female functions of plants by manipulating its pollinators and causing pollinators to adopt a position ideal for pollen removal and receipt. This study provides a novel example of structure adaptation in which both the male and the female functions are enhanced by resource allocation on a male organ, expanding the knowledge of the sexual roles of the anther appendage.
[Mh] Termos MeSH primário: Asarum
Flores/anatomia & histologia
Pólen
Polinização/fisiologia
[Mh] Termos MeSH secundário: Animais
Insetos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.1038/srep22340


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[PMID]:26867602
[Au] Autor:Keerthi D; Aswati Nair R; Prasath D
[Ad] Endereço:School of Biotechnology, National Institute of Technology Calicut (NITC), Calicut, Kerala, India.
[Ti] Título:Molecular phylogenetics and anti-Pythium activity of endophytes from rhizomes of wild ginger congener, Zingiber zerumbet Smith.
[So] Source:World J Microbiol Biotechnol;32(3):41, 2016 Mar.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Zingiber zerumbet, a perennial rhizomatous herb exhibits remarkable disease resistance as well as a wide range of pharmacological activities. Towards characterizing the endophytic population of Z. zerumbet rhizomes, experiments were carried out during two different growing seasons viz., early-June of 2013 and late-July of 2014. A total of 34 endophytes were isolated and categorized into 11 morphologically distinct groups. Fungi were observed to predominate bacterial species with colonization frequency values ranging from 12.5 to 50%. Among the 11 endophyte groups isolated, molecular analyses based on ITS/16S rRNA gene sequences identified seven isolate groups as Fusarium solani, two as F. oxysporum and one as the bacterium Rhizobium spp. Phylogenetic tree clustered the ITS sequences from Z. zerumbet endophytes into distinct clades consistent with morphological and sequence analysis. Dual culture assays were carried out to determine antagonistic activity of the isolated endophytes against Pythium myriotylum, an economically significant soil-borne phytopathogen of cultivated ginger. Experiments revealed significant P. myriotylum growth inhibition by F. solani and F. oxysporum isolates with percentage of inhibition (PoI) ranging from 45.17 ± 0.29 to 62.2 ± 2.58 with F. oxysporum exhibiting higher PoI values against P. myriotylum. Using ZzEF8 metabolite extract, concentration-dependent P. myriotylum hyphal growth inhibition was observed following radial diffusion assays. These observations were confirmed by scanning electron microscopy analysis wherein exposure to ZzEF8 metabolite extract induced hyphal deformities. Results indicate Z. zerumbet endophytes as promising resources for biologically active compounds and as biocontrol agents for soft rot disease management caused by Pythium spp.
[Mh] Termos MeSH primário: Antibiose
Endófitos/classificação
Endófitos/isolamento & purificação
Filogenia
Pythium/crescimento & desenvolvimento
Rizoma/microbiologia
Zingiberaceae/microbiologia
[Mh] Termos MeSH secundário: Asarum/crescimento & desenvolvimento
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Endófitos/genética
Endófitos/crescimento & desenvolvimento
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Zingiberaceae/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Fungal); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-015-1998-8


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[PMID]:26830293
[Au] Autor:Meadley Dunphy SA; Prior KM; Frederickson ME
[Ad] Endereço:Department of Ecology and Evolutionary Biology, University of Toronto, 25 Harbord Street, Toronto, ON, M5S 3G5, Canada. shannon.meadleydunphy@mail.utoronto.ca.
[Ti] Título:An invasive slug exploits an ant-seed dispersal mutualism.
[So] Source:Oecologia;181(1):149-59, 2016 May.
[Is] ISSN:1432-1939
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Plant-animal mutualisms, such as seed dispersal, are often vulnerable to disruption by invasive species. Here, we show for the first time how a non-ant invasive species negatively affects seed dispersal by ants. We examined the effects of several animal species that co-occur in a temperate deciduous forest-including native and invasive seed-dispersing ants (Aphaenogaster rudis and Myrmica rubra, respectively), an invasive slug (Arion subfuscus), and native rodents-on a native myrmecochorous plant, Asarum canadense. We experimentally manipulated ant, slug, and rodent access to seed depots and measured seed removal. We also video-recorded depots to determine which other taxa interact with seeds. We found that A. rudis was the main disperser of seeds and that A. subfuscus consumed elaiosomes without dispersing seeds. Rodent visitation was rare, and rodent exclusion had no significant effect on seed or elaiosome removal. We then used data obtained from laboratory and field mesocosm experiments to determine how elaiosome robbing by A. subfuscus affects seed dispersal by A. rudis and M. rubra. We found that elaiosome robbing by slugs reduced seed dispersal by ants, especially in mesocosms with A. rudis, which picks up seeds more slowly than M. rubra. Taken together, our results show that elaiosome robbing by an invasive slug reduces seed dispersal by ants, suggesting that invasive slugs can have profound negative effects on seed dispersal mutualisms.
[Mh] Termos MeSH primário: Formigas/fisiologia
Asarum/fisiologia
Cadeia Alimentar
Gastrópodes/fisiologia
Roedores/fisiologia
Dispersão de Sementes
Simbiose
[Mh] Termos MeSH secundário: Animais
Espécies Introduzidas
Ontário
Sementes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160203
[St] Status:MEDLINE
[do] DOI:10.1007/s00442-015-3530-0


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[PMID]:26708137
[Au] Autor:Kim JR; Perumalsamy H; Lee JH; Ahn YJ; Lee YS; Lee SG
[Ad] Endereço:Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 565-851, Republic of Korea. jr2004@korea.kr.
[Ti] Título:Acaricidal activity of Asarum heterotropoides root-derived compounds and hydrodistillate constitutes toward Dermanyssus gallinae (Mesostigmata: Dermanyssidae).
[So] Source:Exp Appl Acarol;68(4):485-95, 2016 Apr.
[Is] ISSN:1572-9702
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The acaricidal activity of Asarum heterotropoides root-derived principles, methyleugenol, safrole, 3-carene, α-asarone, pentadecane and A. heterotropoides root steam distillate constituents was tested against poultry red mites Dermanyssus gallinae (De Geer). All active principles were identified by spectroscopic analysis. Results were compared with those of two conventional acaricides, benzyl benzoate and N,N-diethyl-3-methylbenzamide (DEET). Methyleugenol (24 h LC50 = 0.57 µg/cm(2)) and safrole (24 h LC50 = 8.54 µg/cm(2)) were the most toxic compounds toward D. gallinae, followed by 3,4,5-trimethoxytoluene, 3,5-dimethoxytoluene, estragole, α-terpineol, verbenone, eucarvone, linalool, and terpinen-4-ol (LC50 = 15.65-27.88 µg/cm(2)). Methyleugenol was 16.7× and 11.0× more toxic than benzyl benzoate (LC50 = 9.52 µg/cm(2)) and DEET (LC50 = 6.28 µg/cm(2)), respectively; safrole was 1.1× and 0.73× more toxic. Asarum heterotropoides root-derived materials, particularly methyleugenol and safrole, merit further study as potential acaricides. Global efforts to reduce the level of highly toxic synthetic acaricides in indoor environments justify further studies on A. heterotropoides root extract and steam distillate preparations containing the active constituents described as potential contact-action fumigants for the control of mites.
[Mh] Termos MeSH primário: Acaricidas
Asarum/química
Ácaros/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Acaricidas/química
Animais
Galinhas/parasitologia
Extratos Vegetais/química
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acaricides); 0 (Plant Extracts)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151229
[St] Status:MEDLINE
[do] DOI:10.1007/s10493-015-0005-2


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[PMID]:26946834
[Au] Autor:Wang YQ; Jiang XM; Wang Z; Wang W; Liao DF; Yan JY
[Ti] Título:[Analytical Methods with Qualitative HPLC Fingerprint and Quantitative Measurement of Effective Components of Processed Asari Radix et Rhizoma].
[So] Source:Zhong Yao Cai;38(7):1388-92, 2015 Jul.
[Is] ISSN:1001-4454
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: In order to provide the basis for quality standard and processing principle of processed Asari Radix et Rhizoma, an HPLC fingerprint of processed Asari Radix et Rhizoma was established and the contents of methyl eugenol and asarinin were determined. METHODS: The analytical column was Agligent Tc-C18 (250 mm x 4. 6 mm, 5 µm); A mixture of acetonitrile-0. 2% acetic acid solution was used as the mobile phase with gradient elution at the flow rate of 1. 0 mL/min. The wavelength was set at 285 nm and the column temperature was 30 °C. RESULTS: The fingerprint of processed Asari Radix et Rhizoma was established. The asarinin peak was taken as the reference peak. 22 common peaks were assigned, and two peaks were confirmed by comparing with the reference standards. The difference of components was not significant among the various processed products except ginger, honey and fried coke products, but the contents of effective constituents were different among the processed products. The retention rate of methyl eugenol in processed products was in a descending order as follows: wine > vinegar > liquorice > alkali-vinegar > fried coke > rice water system > honey > ginger > salt system > alkali. Methyl eugenol was increased 10% ~ 20% with wine processing and retained more than 95% with vinegar. The retention rate of asarinin in processed products was in declining as: rice water system > liquorice > alkali-vinegar > honey > salt system > wine > ginger > vinegar > alkali > fried coke. The processing techniques increased the content of asarinin except the alkali and fried coke products, and asarinin was increased more than 35% with rice water, alkali-vinegar or liquorice processing. CONCLUSION: The method is accurate and reliable, which can be used for the quality control of processed products of Asari Radix et Rhizoma.
[Mh] Termos MeSH primário: Asarum/química
Cromatografia Líquida de Alta Pressão
Medicamentos de Ervas Chinesas/química
[Mh] Termos MeSH secundário: Dioxóis/análise
Eugenol/análogos & derivados
Eugenol/análise
Lignanas/análise
Raízes de Plantas
Controle de Qualidade
Rizoma
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dioxoles); 0 (Drugs, Chinese Herbal); 0 (Lignans); 29T9VA6R7M (methyleugenol); 3T8H1794QW (Eugenol); S7946O4P76 (sesamin)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160307
[Lr] Data última revisão:
160307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160308
[St] Status:MEDLINE



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