Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.100.041 [Categoria DeCS]
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[PMID]:28829814
[Au] Autor:Rosa JD; Weber GG; Cardoso R; Górski F; Da-Silva PR
[Ad] Endereço:Department of Biological Sciences, Plant Genetics and Molecular Biology Laboratory, Universidade Estadual do Centro-Oeste, UNICENTRO, Guarapuava, Paraná, Brazil.
[Ti] Título:Variability and population genetic structure in Achyrocline flaccida (Weinm.) DC., a species with high value in folk medicine in South America.
[So] Source:PLoS One;12(8):e0183533, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Better knowledge of medicinal plant species and their conservation is an urgent need worldwide. Decision making for conservation strategies can be based on the knowledge of the variability and population genetic structure of the species and on the events that may influence these genetic parameters. Achyrocline flaccida (Weinm.) DC. is a native plant from the grassy fields of South America with high value in folk medicine. In spite of its importance, no genetic and conservation studies are available for the species. In this work, microsatellite and ISSR (inter-simple sequence repeat) markers were used to estimate the genetic variability and structure of seven populations of A. flaccida from southern Brazil. The microsatellite markers were inefficient in A. flaccida owing to a high number of null alleles. After the evaluation of 42 ISSR primers on one population, 10 were selected for further analysis of seven A. flaccida populations. The results of ISSR showed that the high number of exclusive absence of loci might contribute to the inter-population differentiation. Genetic variability of the species was high (Nei's diversity of 0.23 and Shannon diversity of 0.37). AMOVA indicated higher genetic variability within (64.7%) than among (33.96%) populations, and the variability was unevenly distributed (FST 0.33). Gene flow among populations ranged from 1.68 to 5.2 migrants per generation, with an average of 1.39. The results of PCoA and Bayesian analyses corroborated and indicated that the populations are structured. The observed genetic variability and population structure of A. flaccida are discussed in the context of the vegetation formation history in southern Brazil, as well as the possible anthropogenic effects. Additionally, we discuss the implications of the results in the conservation of the species.
[Mh] Termos MeSH primário: Achyrocline/genética
Medicina Tradicional
Plantas Medicinais
[Mh] Termos MeSH secundário: Alelos
Primers do DNA
DNA de Plantas/genética
Marcadores Genéticos
Genética Populacional
Repetições de Microssatélites/genética
América do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Plant); 0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183533


  2 / 47 MEDLINE  
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[PMID]:28185949
[Au] Autor:Souza CF; Baldissera MD; Cossetin LF; Dalla Lana DF; Monteiro SG
[Ad] Endereço:Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
[Ti] Título:Achyrocline satureioides essential oil loaded in nanocapsules ameliorate the antioxidant/oxidant status in heart of rats infected with Trypanosoma evansi.
[So] Source:Microb Pathog;105:30-36, 2017 Apr.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Oxidative stress has been considered as a pathological mechanism that contributes to initiation and progression of cardiac injury during the Trypanosoma evansi infection. In this sense, the natural compounds with antioxidant and free radical scavenger abilities, such the Achyrocline satureioides essential oil loaded in nanocapsules (AS-NC), may be considered important approach to minimize the cardiac damage. Thus, the aim of this study was to investigate whether AS-NC treatment is able to prevents or reduce the cardiac oxidative damage in infected rats with T. evansi. Heart samples from rats infected by T. evansi showed increased reactive oxygen species (ROS), thiobarbituric reactive-acid substances (TBARS) and glutathione reduced (GSH) levels, while catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities decreased compared with uninfected and untreated animals. Also, the seric biomarkers of cardiac function increased in the infected animals compared with uninfected animals. AS-NC treatment reduced ROS and TBARS levels, ameliorate cardiac CAT and SOD activities of infected rats, and reduced seric biomarkers of cardiac function. AS-NC treatment protected the heart from oxidative stress caused by T. evansi, which might be due to its antioxidant properties. AS-NC might be considered a promising therapeutic agent against oxidative stress, when associated with nanotechnology.
[Mh] Termos MeSH primário: Achyrocline/química
Antioxidantes/farmacologia
Miocárdio/patologia
Nanocápsulas/administração & dosagem
Óleos Voláteis/farmacologia
Tripanossomíase/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antioxidantes/administração & dosagem
Catalase/análise
Feminino
Óleos Voláteis/administração & dosagem
Oxidantes
Ratos Wistar
Superóxido Dismutase/análise
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Nanocapsules); 0 (Oils, Volatile); 0 (Oxidants); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


  3 / 47 MEDLINE  
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[PMID]:28027942
[Au] Autor:Ritter CS; Baldissera MD; Grando TH; Souza CF; Sagrillo MR; da Silva AP; Moresco RN; Guarda NS; da Silva AS; Stefani LM; Monteiro SG
[Ad] Endereço:Cell Culture Laboratory, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
[Ti] Título:Achyrocline satureioides essential oil-loaded in nanocapsules reduces cytotoxic damage in liver of rats infected by Trypanosoma evansi.
[So] Source:Microb Pathog;103:149-154, 2017 Feb.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate whether the treatment with Achyrocline satureioides essential oil-loaded in nanocapsules (AS-NC) is able to protect the hepatic tissue against cytotoxic damage caused by Trypanosoma evansi. Thus, the rats were divided into four groups (n = 6 per group): uninfected/saline, uninfected/AS-NC, infected/saline, and infected/AS-NC. At day 4 post-infection (PI), the animals were euthanized and liver and sera samples were collected to perform the hepatic cell viability assay, and to determine seric levels of reactive oxygen species (ROS) and nitric oxide metabolites (NOx). Cell viability decreased (p < 0.05) in the infected/saline group compared to uninfected/saline group, while the treatment with AS-NC avoided this alteration in infected rats. Seric ROS and NOx levels increased (p < 0.05) in the infected/saline group compared to uninfected/saline group, while the treatment with AS-NC avoided this effect on ROS levels of infected rats. In summary, the treatment with AS-NC was able to protect the liver tissue against the cytotoxic effect caused by the parasite by avoiding exacerbated production of ROS.
[Mh] Termos MeSH primário: Achyrocline/química
Fígado/patologia
Fígado/parasitologia
Nanocápsulas/administração & dosagem
Óleos Voláteis/administração & dosagem
Trypanosoma/efeitos dos fármacos
Tripanossomíase/patologia
Tripanossomíase/parasitologia
[Mh] Termos MeSH secundário: Animais
Feminino
Fígado/efeitos dos fármacos
Nanocápsulas/química
Nanocápsulas/toxicidade
Nanocápsulas/ultraestrutura
Óxido Nítrico/metabolismo
Óleos Voláteis/química
Óleos Voláteis/toxicidade
Extratos Vegetais/química
Ratos
Espécies Reativas de Oxigênio/metabolismo
Tripanossomíase/tratamento farmacológico
Tripanossomíase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nanocapsules); 0 (Oils, Volatile); 0 (Plant Extracts); 0 (Reactive Oxygen Species); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE


  4 / 47 MEDLINE  
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[PMID]:27599114
[Au] Autor:Balestrin LA; Bidone J; Bortolin RC; Moresco K; Moreira JC; Teixeira HF
[Ad] Endereço:Programa de Pós-graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90610-000, Brazil.
[Ti] Título:Protective effect of a hydrogel containing Achyrocline satureioides extract-loaded nanoemulsion against UV-induced skin damage.
[So] Source:J Photochem Photobiol B;163:269-76, 2016 Oct.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Achyrocline satureioides is a medicinal plant widely used in South America that exhibits a well-documented antioxidant activity. Such activity has been related to their main aglycone flavonoids quercetin, luteolin, and 3-O-methylquercetin (3MQ). This study addresses the development of antioxidant hydrogels containing an A. satureioides extract-loaded nanoemulsions aimed at topical application. The systems investigated were A. satureioides extract-loaded nanoemulsions (ASNE) obtained by spontaneous emulsification procedure formulated in semisolid hydrogels composed of Carbopol® Ultrez 20 (HASNE). Hydrogels exhibit a non-Newtonian pseudoplastic behavior. A higher release of 3MQ from ASNE (3.61µg/cm(2)/h) was observed when compared with HASNE (2.83µg/cm(2)/h). Different parameters that may have an influence on the retention of flavonoids into the skin were investigated by using a Franz-type diffusion cells. Indeed, the amount of formulation applied on donor compartment was found to play a crucial role. At the optimized conditions, retention of approximately 2µg/cm(2) of flavonoids was detected into the skin. A higher retention of 3MQ was detected (approximately 1.0µg/cm(2)) in comparison with the other flavonoids. Finally, a protection the porcine ear skin by formulations, against oxidative stress generated by UVA/UVB light was demonstrated by means of TBARS, protein carbonylation, and protein thiol content assays. The overall results showed the potential of the formulations developed in this study for the prevention of oxidative stress on the skin.
[Mh] Termos MeSH primário: Achyrocline/química
Hidrogéis/química
Nanoestruturas/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Pele/efeitos dos fármacos
Pele/efeitos da radiação
[Mh] Termos MeSH secundário: Antioxidantes/química
Antioxidantes/metabolismo
Antioxidantes/farmacologia
Composição de Medicamentos
Liberação Controlada de Fármacos
Emulsões
Flavonoides/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Permeabilidade/efeitos dos fármacos
Permeabilidade/efeitos da radiação
Extratos Vegetais/metabolismo
Protetores contra Radiação/química
Protetores contra Radiação/metabolismo
Protetores contra Radiação/farmacologia
Pele/citologia
Pele/metabolismo
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Emulsions); 0 (Flavonoids); 0 (Hydrogels); 0 (Plant Extracts); 0 (Radiation-Protective Agents)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160907
[St] Status:MEDLINE


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[PMID]:27575777
[Au] Autor:Salgueiro AC; Folmer V; da Rosa HS; Costa MT; Boligon AA; Paula FR; Roos DH; Puntel GO
[Ad] Endereço:Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, Uruguaiana, Rio Grande do Sul, Brazil. Electronic address: acfsalgueiro@gmail.com.
[Ti] Título:In vitro and in silico antioxidant and toxicological activities of Achyrocline satureioides.
[So] Source:J Ethnopharmacol;194:6-14, 2016 Dec 24.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Achyrocline satureioides ("macela or marcela") is a medicinal plant, traditionally collected in "Good Friday" before sunrise. In traditional medicine, dried flowers of A. satureioides are used as anti-dyspeptic, antispasmodic and anti-inflammatory. AIM OF THE STUDY: To evaluate the phytochemical profile and to present an in vitro and in silico approach about toxicity and antioxidant potential of A. satureioides flowers extract and its major phytoconstituents. MATERIALS AND METHODS: Plant were collected according to the popular tradition. Extract were obtained by infusion and analyzed from high-performance liquid chromatography. Toxicity was evaluated in Artemia salina and human lymphocytes. Extract antioxidant activity was determined with total antioxidant capacity, DPPH and ABTS scavenging, ferric reducing antioxidant power, deoxyribose degradation assay, and thiobarbituric acid reactive substances (TBA-RS) assay. TBA-RS inhibitions were evaluated in brain of rats for A. satureioides extract and its major phytoconstituents. Predictions of activity spectra for substances and in silico toxicity evaluation from major phytoconstituents were performed via computer simulation. RESULTS: Chromatographic data indicated isoquercitrin, quercetin and caffeic acid as main compounds in flowers extract. Toxicity tests demonstrated a very low toxic potential of A. satureioides. Extract exhibited antioxidant activities in low concentrations. Both extract and major phytochemicals standards showed protection against lipid peroxidation in brain of rats. Computer simulations pointed some biological activities in agreement with traditional use, as well as some experimental results found in this work. Moreover, in silico toxic predictions showed that the A. satureioides major compounds had low probability for toxic risk. CONCLUSION: Our results indicate that A. satureioides infusion possesses low toxicological potential and an effective antioxidant activity. These findings confirm the traditional use of this plant in the folk medicine.
[Mh] Termos MeSH primário: Achyrocline/química
Antioxidantes/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Artemia/efeitos dos fármacos
Células Cultivadas
Cromatografia Líquida de Alta Pressão
Ensaio Cometa
Seres Humanos
Limite de Detecção
Extratos Vegetais/toxicidade
Ratos
Espectrofotometria Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE


  6 / 47 MEDLINE  
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[PMID]:26361953
[Au] Autor:Zorzi GK; Caregnato F; Moreira JC; Teixeira HF; Carvalho EL
[Ad] Endereço:Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil.
[Ti] Título:Antioxidant Effect of Nanoemulsions Containing Extract of Achyrocline satureioides (Lam) D.C.-Asteraceae.
[So] Source:AAPS PharmSciTech;17(4):844-50, 2016 Aug.
[Is] ISSN:1530-9932
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ethanolic extracts of Achyrocline satureioides have pronounced antioxidant activity mainly due to the presence of the flavonoid quercetin. However, direct topical application of the extract is not possible due to the presence of high amounts of ethanol. In this sense, nanoemulsions arise as an alternative for topical formulation associating molecules with limited aqueous solubility. This article describes the development of topical nanoemulsions containing either A. satureioides extract or one of its most abundant flavonoid, quercetin. Nanoemulsions composed of octyldodecanol, egg lecithin, water and extract (NEE), or quercetin (NEQ) were prepared by spontaneous emulsification. This process led to monodisperse nanoemulsions presenting a mean droplet size of approximately 200-300 nm, negative zeta potential, and high association efficiency. A study of quercetin skin retention using porcine skin which was performed using a Franz diffusion cell revealed a higher accumulation of quercetin in skin for NEE when compared to NEQ. Finally, the antioxidant activity of formulations was measured by thiobarbituric acid-reactive species and the APPH model. A lower lipoperoxidation for the extract in respect to quercetin solution was observed. However, no difference between NEQ and NEE lipoperoxidation could be seen. The protection against lipoperoxidation by the formulations was also measured in the skin, where lower formation of reactive species was observed after treatment with NEE. In conclusion, this study shows the formulation effect on the physicochemical properties of nanoemulsions as well as on the skin retention and antioxidant activity of quercetin.
[Mh] Termos MeSH primário: Achyrocline/química
Antioxidantes/farmacologia
Asteraceae/química
Emulsões/farmacologia
Nanopartículas/administração & dosagem
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Química Farmacêutica/métodos
Emulsões/química
Álcoois Graxos/química
Flavonoides/química
Flavonoides/farmacologia
Nanopartículas/química
Extratos Vegetais/química
Quercetina/química
Quercetina/farmacologia
Pele/metabolismo
Absorção Cutânea/efeitos dos fármacos
Solubilidade
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Emulsions); 0 (Fatty Alcohols); 0 (Flavonoids); 0 (Plant Extracts); 461N1O614Y (octyl dodecanol); 9IKM0I5T1E (Quercetin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150913
[St] Status:MEDLINE
[do] DOI:10.1208/s12249-015-0408-8


  7 / 47 MEDLINE  
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[PMID]:26630872
[Au] Autor:Téllez MA; Téllez AN; Vélez F; Ulloa JC
[Ad] Endereço:Laboratorio de Virología, Departamento de Microbiología, Facultad de Ciencias, Grupo de Enfermedades Infecciosas, Pontificia Universidad Javeriana, Cra. 7 # 43-82, Bogotá, Colombia.
[Ti] Título:In vitro antiviral activity against rotavirus and astrovirus infection exerted by substances obtained from Achyrocline bogotensis (Kunth) DC. (Compositae).
[So] Source:BMC Complement Altern Med;15(1):428, 2015 Dec 03.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Achyrocline bogotensis has been traditionally used to treat infections of skin, respiratory, tract urinary and other infections, but not to treat viral gastrointestinal disease. In this study, this Colombian native medicinal plant was investigated by its in vitro anti-rotavirus and anti-astrovirus activity. METHODS: Several extracts and fractions phytochemically obtained from A. bogotensis were evaluated initially for their cell toxicity on MA104 and Caco2 cells and then for their anti-rotavirus (RRV) and anti-astrovirus (Yuc8) activity following three strategies: pre-treatment of cells (blocking effect), direct viral activity (virucidal effect) and post-treatment of infected cells (reduction of viral yield post-infection). In addition qualitative chemical studies were developed for the active compounds. RESULTS: Non-toxic concentrations of a fraction obtained exhibited antiviral activity against both viruses characterized by a virucidal effect and by the reduction of the infectious particles produced post-infection. Steroids, sterols, terpenes, phenols, flavonoids and sesquiterpenlactones were identified qualitatively in the active fraction. CONCLUSIONS: A. bogotensis contains substances with in vitro antiviral activity against rotavirus and astrovirus. This study confirms their anti-microbial properties and describes by the first time its antiviral activity in vitro.
[Mh] Termos MeSH primário: Achyrocline
Antivirais/farmacologia
Astroviridae/efeitos dos fármacos
Extratos Vegetais/farmacologia
Infecções por Vírus de RNA/tratamento farmacológico
Rotavirus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Achyrocline/química
Achyrocline/toxicidade
Linhagem Celular
Sobrevivência Celular
Seres Humanos
Fitoterapia
Componentes Aéreos da Planta/química
Extratos Vegetais/toxicidade
Plantas Medicinais/química
Plantas Medicinais/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:151205
[Lr] Data última revisão:
151205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151204
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-015-0949-0


  8 / 47 MEDLINE  
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Simöes, Cláudia Maria Oliveira
PubMed Central Texto completo
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[PMID]:26101767
[Au] Autor:Bidone J; Argenta DF; Kratz J; Pettenuzzo LF; Horn AP; Koester LS; Bassani VL; Simões CM; Teixeira HF
[Ad] Endereço:Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Ipiranga 2752, 90610-000 Porto Alegre, RS, Brazil.
[Ti] Título:Antiherpes Activity and Skin/Mucosa Distribution of Flavonoids from Achyrocline satureioides Extract Incorporated into Topical Nanoemulsions.
[So] Source:Biomed Res Int;2015:238010, 2015.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the inhibitory effects of Achyrocline satureioides extract (ASE) incorporated into a topical nanoemulsion on Herpes Simplex Virus type 1 (HSV-1/KOS strain) replication, as well as the distribution of the main ASE flavonoids (quercetin, luteolin, and 3-O-methylquercetin) in porcine skin and mucosa. The ASE-loaded nanoemulsion showed more pronounced effects against HSV-1 replication when compared to the ASE or pure quercetin, as determined by the viral plaque number reduction assay. All flavonoids were detected in the skin epidermis (2.2 µg/cm(2)) and the mucosa upper layers (3.0 µg/cm(2)) from ASE-loaded nanoemulsion until 8 h after topical application. A higher amount of flavonoids was detected when these tissues were impaired, especially in deeper mucosa layers (up to 7-fold). Flavonoids were detected in the receptor fluid only when the mucosa was injured. Such results were supported by confocal microscopy images. Overall, these findings suggest that the tested ASE-loaded nanoemulsion has potential to be used topically for herpes infections.
[Mh] Termos MeSH primário: Emulsões/administração & dosagem
Flavonoides/administração & dosagem
Membrana Mucosa/efeitos dos fármacos
Pele/efeitos dos fármacos
[Mh] Termos MeSH secundário: Achyrocline/química
Administração Tópica
Animais
Emulsões/química
Flavonoides/química
Extratos Vegetais/administração & dosagem
Extratos Vegetais/química
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Emulsions); 0 (Flavonoids); 0 (Plant Extracts)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150624
[St] Status:MEDLINE
[do] DOI:10.1155/2015/238010


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[PMID]:26078941
[Au] Autor:Cariddi LN; Sabini MC; Escobar FM; Bacchetti R; Montironi I; Merckis C; Reinoso EB; Núñez Montoya S; Zanon SM; Comini LR; Sabini LI
[Ad] Endereço:Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, Río Cuarto, C5800 Córdoba, Argentina ; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, C1033AAJ Buenos Aires, Argentina.
[Ti] Título:In Vitro and In Vivo Cytogenotoxic Effects of Hot Aqueous Extract of Achyrocline satureioides (Lam.) DC.
[So] Source:Biomed Res Int;2015:270973, 2015.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this work we extend the toxicological studies of hot aqueous extract of A. satureioides (As-HAE) evaluating cytotoxic and apoptotic effects on human peripheral blood mononuclear cells (PBMCs). We also determine genotoxic action of this extract in vivo. In addition, the extract was chemically characterized. Finally, we established a comparison with previous data of cold aqueous extract. The As-HAE induced cytotoxicity on PBMCs determined by trypan blue dye exclusion (CC50 = 653 µg/mL) and MTT (CC50 = 588 µg/mL) assays being more toxic than cold extract. However, As-HAE as well as cold extract did not induce apoptosis measured by Hoechst 33258 staining, TUNEL assay, and DNA fragmentation analysis. The in vivo micronucleus test showed that As-HAE exerted cytogenotoxic effects on bone marrow of mice, contrary to what was observed with cold extract. The chemical study of As-HAE allowed identifying the flavonoids found in cold extract: luteolin, quercetin, and 3-O-methylquercetin, but at higher concentrations. We suggest that toxic effects induced by As-HAE could be due to high concentrations of these flavonoids. Given that As-HAE is the most used in folkloric medicine, its administration should be controlled in order to prevent potential cell damage.
[Mh] Termos MeSH primário: Flavonoides/farmacologia
Luteolina/farmacologia
Extratos Vegetais/farmacologia
Quercetina/análogos & derivados
[Mh] Termos MeSH secundário: Achyrocline/química
Animais
Apoptose/efeitos dos fármacos
Dano ao DNA/efeitos dos fármacos
Fragmentação do DNA/efeitos dos fármacos
Flavonoides/química
Seres Humanos
Leucócitos Mononucleares/efeitos dos fármacos
Luteolina/isolamento & purificação
Camundongos
Extratos Vegetais/química
Quercetina/isolamento & purificação
Quercetina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Flavonoids); 0 (Plant Extracts); 07X3IB4R4Z (3-methylquercetin); 9IKM0I5T1E (Quercetin); KUX1ZNC9J2 (Luteolin)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150617
[St] Status:MEDLINE
[do] DOI:10.1155/2015/270973


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[PMID]:25820295
[Au] Autor:González MJ; Beoletto VG; Agnese AM; Audisio MC; Marioli JM
[Ad] Endereço:Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina.
[Ti] Título:Purification of substances from Achyrocline satureioides with inhibitory activity against Paenibacillus larvae, the causal agent of American foulbrood in honeybees' larvae.
[So] Source:Appl Biochem Biotechnol;175(7):3349-59, 2015 Apr.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Achyrocline satureioides extracts were tested in vitro against the growth of Paenibacillus larvae. Four different extracts were obtained by liquid-liquid extraction from an aqueous-ethyl alcohol macerate of the aerial parts of the plant. The biological activity was tested by the broth microdilution technique. Hexane extract showed the highest activity (minimum inhibitory concentration = 0.060 ± 0.037 mg/mL). Transmission electron microscopy experiments showed that the main effect exerted by the hexane extract on the cell was at the cellular membrane level. The hexane extract was analyzed by thin-layer chromatography, and the activity of its components was tested by bioautography. Four growth inhibition zones were observed in the bioautographic experiments (using hexane-acetone (7:3) as mobile phase), with that at Rf = 0.57 showing the largest zone of inhibition. High-performance liquid chromatographic experiments, using ultraviolet and electrospray ionization coupled to tandem mass spectrometric detection, showed the presence of one compound with a m/z ratio of 442, which may be related to phloroglucinols α-pyrone compounds recently discovered. The high antibacterial activity of the hexane extract and of the isolated compound determined in this work may be useful for the development of future new alternatives for the treatment of American foulbrood.
[Mh] Termos MeSH primário: Paenibacillus/efeitos dos fármacos
Extratos Vegetais/farmacologia
Pironas/farmacologia
[Mh] Termos MeSH secundário: Achyrocline/química
Animais
Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Abelhas/microbiologia
Larva/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Paenibacillus/patogenicidade
Extratos Vegetais/química
Pironas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Extracts); 0 (Pyrones)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150330
[Lr] Data última revisão:
150330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150331
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-015-1506-5



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