[PMID]: | 28841693 |
[Au] Autor: | Zhang A; Wang D; Li J; Gao F; Fan X |
[Ad] Endereço: | Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China. |
[Ti] Título: | The effect of aqueous extract of Xinjiang Artemisia rupestris L. (an influenza virus vaccine adjuvant) on enhancing immune responses and reducing antigen dose required for immunity. |
[So] Source: | PLoS One;12(8):e0183720, 2017. |
[Is] ISSN: | 1932-6203 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Potent adjuvant can improve the effectiveness of vaccines and reduce the antigen doses required for initiating the protective immunity. In this study, we identified that aqueous extract of Artemisia rupestris L. (AEAR) could be employed as an efficient adjuvant for influenza virus vaccine (V) to enhance immune responses and reduce the antigen doses required for initiating immunity, without compromising the immune response. ICR mice were subcutaneously co-administrated with V combined with different concentrations of AEAR demonstrated that 300 µg AEAR could significantly improve hemagglutination inhibition (HI) and increase IgG antibody titers in serum (P<0.05) and the population of CD4+CD44+ and CD8+CD44+ (P<0.05). Next, 300 µg AEAR combined with different doses of V in vivo markedly increased HI and specific IgG antibody level(P<0.05). It also significantly increased the amount of CD4+ and CD8+ T cells, CD4+CD44+ and CD8+CD44+ T cells (P<0.05), improved lymphocyte proliferation, the secretion of CD4+IL-4, CD4+IFN-γ and CD8+IFN-γ (P<0.05), and the killing efficacy of cytotoxic T lymphocyte (CTL) (P<0.05). Furthermore, the combination increased the expression of major histocompatibility complex-II (MHC-II) and co-stimulatory molecules including CD40, CD80, and CD86 on dendritic cells (DCs), and downregulated the expression of CD25+Foxp3+Treg cells (P<0.05). No significant difference was observed between high-dose V and low-dose AEAR-V (10-fold lower) vaccination group (P>0.05), indicating a 10-fold reduction of antigen required for V vaccine administration. In conclusion, this study demonstrated that AEAR, as an adjuvant for influenza vaccine, could stimulate potent humoral and cellular immune responses and reduce the antigen dose required for effective vaccination, which were mediated by promoting DCs activation and repressing Treg expression. |
[Mh] Termos MeSH primário: |
Adjuvantes Imunológicos/farmacologia Antígenos/administração & dosagem Artemisia/química Extratos Vegetais/farmacologia
|
[Mh] Termos MeSH secundário: |
Proliferação Celular Citocinas/metabolismo Citotoxicidade Imunológica Relação Dose-Resposta Imunológica Ensaio de Imunoadsorção Enzimática Imunoglobulina G/sangue Linfócitos T/citologia Linfócitos T/imunologia Água
|
[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Adjuvants, Immunologic); 0 (Antigens); 0 (Cytokines); 0 (Immunoglobulin G); 0 (Plant Extracts); 059QF0KO0R (Water) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171026 |
[Lr] Data última revisão:
| 171026 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170826 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1371/journal.pone.0183720 |
|
|