Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.100.227 [Categoria DeCS]
Referências encontradas : 140 [refinar]
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  1 / 140 MEDLINE  
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[PMID]:28851651
[Au] Autor:Lee S; Lee DH; Kim JC; Um BH; Sung SH; Jeong LS; Kim YK; Kim SN
[Ad] Endereço:Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea.
[Ti] Título:Pectolinarigenin, an aglycone of pectolinarin, has more potent inhibitory activities on melanogenesis than pectolinarin.
[So] Source:Biochem Biophys Res Commun;493(1):765-772, 2017 Nov 04.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pectolinarin and pectolinarigenin have been reported to be major compounds in Cirsium setidens. In the present study, we demonstrated inhibitory effects of pectolinarin and pectolinarigenin from C. setidens on melanogenesis. Melanin synthesis was decreased in both pectolinarin- and pectolinarigenin-treated melan-a cells and in a reconstructed human skin model. However, pectolinarigenin treatment showed more potent inhibitory activity of melanin synthesis than did pectolinarin treatment. The concentrations of pectolinarin and pectolinarigenin in C. setidens water extracts were determined by HPLC. Unfortunately, the amount of pectolinarigenin of C. setidens water extract was lower than that of pectolinarin. To increase the pectolinarigenin content in C. setidens water extract, several component conversion methods were studied. Consequently, we identified that microwave irradiation under 1% acetic acid was an optimum sugar elimination method.
[Mh] Termos MeSH primário: Cromonas/administração & dosagem
Cirsium/química
Melaninas/biossíntese
Pele/efeitos dos fármacos
Pele/metabolismo
[Mh] Termos MeSH secundário: Células Cultivadas
Relação Dose-Resposta a Droga
Seres Humanos
Iridoides/administração & dosagem
Extratos Vegetais/administração & dosagem
Pele/citologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromones); 0 (Iridoids); 0 (Melanins); 0 (Plant Extracts); 0 (pectolinarigenin); 28978-02-1 (pectolinarin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE


  2 / 140 MEDLINE  
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[PMID]:28784292
[Au] Autor:Yeon Park J; Young Kim H; Shibamoto T; Su Jang T; Cheon Lee S; Suk Shim J; Hahm DH; Lee HJ; Lee S; Sung Kang K
[Ad] Endereço:College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea.
[Ti] Título:Beneficial effects of a medicinal herb, Cirsium japonicum var. maackii, extract and its major component, cirsimaritin on breast cancer metastasis in MDA-MB-231 breast cancer cells.
[So] Source:Bioorg Med Chem Lett;27(17):3968-3973, 2017 09 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The biological activities of the ethanol extract from Cirsium japonicum var. maackii (ICF-1) and its major component, polyphenol cirsimaritin, were investigated as part of the search for possible alternative drugs for breast cancer. Three in vitro cell-based assays were used: the cell proliferation assay, tube-formation assay, and Western blot analysis. Both the ICF-1 extract and cirsimaritin inhibited the viability of HUVECs in a dose-dependent manner. The inhibition achieved was 36.89% at a level of 200µg/ml by the ICF-1 extract and 62.04% at a level of 100µM by cirsimaritin. The ICF-1 extract and cirsimaritin reduced tube formation by 12.69% at level of 25µg/ml and 32.18% at the levels of 6.25µM, respectively. Cirsimaritin inhibited angiogenesis by downregulation of VEGF, p-Akt and p-ERK in MDA-MB-231 cells, suggesting that cirsimaritin is potentially useful as an anti-metastatic agent. The present study demonstrated that Cirsium japonicum extract and its active component cirsimaritin is an excellent candidate as an alternative anti-breast cancer drug.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Neoplasias da Mama/tratamento farmacológico
Cirsium/química
Flavonas/farmacologia
Neovascularização Patológica/tratamento farmacológico
Extratos Vegetais/farmacologia
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Neoplasias da Mama/patologia
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Feminino
Flavonas/química
Flavonas/isolamento & purificação
Seres Humanos
Estrutura Molecular
Neovascularização Patológica/patologia
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Flavones); 0 (Plant Extracts); 6601-62-3 (cirsimaritin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE


  3 / 140 MEDLINE  
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[PMID]:28554870
[Au] Autor:Shin MS; Park JY; Lee J; Yoo HH; Hahm DH; Lee SC; Lee S; Hwang GS; Jung K; Kang KS
[Ad] Endereço:Natural Constituents Research Center, Korea Institute of Science and Technology Gangneung, Institute of Natural Products, 210-340, Republic of Korea.
[Ti] Título:Anti-inflammatory effects and corresponding mechanisms of cirsimaritin extracted from Cirsium japonicum var. maackii Maxim.
[So] Source:Bioorg Med Chem Lett;27(14):3076-3080, 2017 07 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, we investigated the anti-inflammatory effects and mechanisms of cirsimaritin isolated from an ethanol extract of the aerial parts of Cirsium japonicum var. maackii Maxim. using RAW264.7 cells. The extract and its flavonoid cirsimaritin inhibited nitric oxide (NO) production and inducible nitric oxide synthase expression in RAW264.7 cells. Cirsimaritin inhibited interleukin-6, tumor necrosis factor-α, and NO production in a concentration-dependent manner in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. From a western blot study, pretreatment with cirsimaritin inhibited phosphorylation/degradation of IκBα and phosphorylation of Akt in LPS-stimulated RAW264.7 cells. Moreover, cirsimaritin suppressed activation of LPS-induced transcription factors, such as c-fos and signal transducer and activator of transcription 3 (STAT3), in RAW264.7 cells. Collectively, these results show that cirsimaritin possesses anti-inflammatory activity, which is regulated by inhibition of c-fos and STAT3 phosphorylation in RAW264.7 cells.
[Mh] Termos MeSH primário: Anti-Inflamatórios/química
Cirsium/química
Flavonas/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/farmacologia
Cirsium/metabolismo
Flavonas/isolamento & purificação
Flavonas/farmacologia
Interleucina-6/metabolismo
Lipopolissacarídeos/toxicidade
Macrófagos/citologia
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
Camundongos
Inibidor de NF-kappaB alfa/metabolismo
Óxido Nítrico/metabolismo
Óxido Nítrico Sintase Tipo II/metabolismo
Fosforilação/efeitos dos fármacos
Componentes Aéreos da Planta/química
Componentes Aéreos da Planta/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Proteínas Proto-Oncogênicas c-fos/metabolismo
Células RAW 264.7
Fator de Transcrição STAT3/metabolismo
Transdução de Sinais/efeitos dos fármacos
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Flavones); 0 (Interleukin-6); 0 (Lipopolysaccharides); 0 (Plant Extracts); 0 (Proto-Oncogene Proteins c-fos); 0 (STAT3 Transcription Factor); 0 (Stat3 protein, mouse); 0 (Tumor Necrosis Factor-alpha); 139874-52-5 (NF-KappaB Inhibitor alpha); 31C4KY9ESH (Nitric Oxide); 6601-62-3 (cirsimaritin); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE


  4 / 140 MEDLINE  
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[PMID]:28088737
[Au] Autor:Chang N; Li Y; Zhou M; Gao J; Hou Y; Jiang M; Bai G
[Ad] Endereço:Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China.
[Ti] Título:The hemostatic effect study of Cirsium setosum on regulating α1-ARs via mediating norepinephrine synthesis by enzyme catalysis.
[So] Source:Biomed Pharmacother;87:698-704, 2017 Mar.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cirsium setosum (CS) is the aboveground part of Cephalanoplos segetum Kitam. Although it has been used as a hemostatic treatment for thousands of years and is still in use today, the mechanism of CS on regulating ARs is still not clear. PURPOSE: In this study, we aimed to clarify the mechanism of CS on regulating ARs. METHODS: We developed a simple method based on UPLC/Q-TOF MS combined adrenergic receptor dual-luciferase reporter assay systems for the rapid determination of active constituents in CS. The mechanism of tyramine, the main active component for regulating ARs, was further investigated by an in vitro norepinephrine biotransformation test and in vivo vaso activity tests. RESULTS: Two phenethylamine ARs regulators (tyramine and N-methyltyramine) in CS were characterized, and it was found that tyramine could induce vasoconstriction via regulation of α1-ARs by mediating norepinephrine synthesis. CONCLUSION: The hemostatic effect of CS is associated with tyramine and N-methyltyramine, via regulation of α1-ARs, and the mechanism of tyramine is related to mediating norepinephrine synthesis by enzyme catalysis.
[Mh] Termos MeSH primário: Catálise/efeitos dos fármacos
Cirsium/química
Hemostáticos/farmacologia
Norepinefrina/metabolismo
Extratos Vegetais/farmacologia
Receptores Adrenérgicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Células HEK293
Seres Humanos
Masculino
Ratos
Ratos Sprague-Dawley
Tiramina/análogos & derivados
Tiramina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hemostatics); 0 (Plant Extracts); 0 (Receptors, Adrenergic); G3S4E2F7TA (methyl-4-tyramine); X4W3ENH1CV (Norepinephrine); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170710
[Lr] Data última revisão:
170710
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170116
[St] Status:MEDLINE


  5 / 140 MEDLINE  
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[PMID]:27599894
[Au] Autor:Lee JH; Jung HK; Han YS; Yoon YM; Yun CW; Sun HY; Cho HW; Lee SH
[Ad] Endereço:Department of Physiology, Laboratory for Vascular Medicine and Stem Cell Biology, Medical Research Institute, School of Medicine, Pusan National University, Yangsan, South Gyeongsang 50612, Republic of Korea.
[Ti] Título:Antioxidant effects of Cirsium setidens extract on oxidative stress in human mesenchymal stem cells.
[So] Source:Mol Med Rep;14(4):3777-84, 2016 Oct.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Human mesenchymal stem cells (MSCs) may be used in cell-based therapy to promote neovascularization for the treatment of ischemic diseases. However, high levels of reactive oxygen species (ROS) derived from the pathophysiological ischemic environment induce senescence and apoptosis of MSCs, resulting in reduced functionality and defective neovascularization. Therefore, the present study aimed to determine the protective effects of Cirsium setidens, a natural product, on oxidative stress­induced apoptosis in MSCs. The present study investigated for the change of ROS levels in MSCs using ROS assays. In addition, cell viability determined by MTT and TUNEL assays. Western blot analysis was performed to investigate the change of apoptosis­associated proteins in MSCs. Treatment of MSCs with hydrogen peroxide (H2O2; 200 µM) significantly increased intracellular ROS levels and cell death; however, pretreatment with C. setidens (100 µg/ml) suppressed H2O2­induced ROS generation and increased the survival of MSCs. H2O2­induced ROS production increased the levels of phosphorylated­p38 mitogen activated protein kinase, c­Jun N­terminal kinase, ataxia telangiectasia mutated and p53; these increases were inhibited by pretreatment with C. setidens. In addition, C. setidens inhibited ROS­induced apoptosis of MSCs by increasing the expression levels of the anti­apoptotic protein B­cell lymphoma 2 (BCL­2), and decreasing the expression levels of the proapoptotic protein BCL­2­associated X protein. These findings indicated that pretreatment of MSCs with C. setidens may prevent ROS­induced oxidative injury by regulating the oxidative stress­associated signaling pathway, and suppressing the apoptosis­associated signal pathway. Therefore, C. setidens may be developed as a beneficial broad­spectrum agent for enhancing the effectiveness of MSC transplantation in the treatment of ischemic diseases.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Cirsium/química
Células Mesenquimais Estromais/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/química
Apoptose/efeitos dos fármacos
Proteínas Reguladoras de Apoptose/metabolismo
Linhagem Celular
Seres Humanos
Peróxido de Hidrogênio/metabolismo
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/metabolismo
Extratos Vegetais/química
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Apoptosis Regulatory Proteins); 0 (Plant Extracts); 0 (Reactive Oxygen Species); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5706


  6 / 140 MEDLINE  
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[PMID]:27598298
[Au] Autor:Luan N; Wei WD; Wang A; Wu XL; Qi Y; Li JJ; Zheng JQ; Shang XY
[Ad] Endereço:a Beijing Key Laboratory of Bioactive Substances and Functional Foods , Beijing Union University , Beijing 100191 , China.
[Ti] Título:Four new taraxastane-type triterpenoic acids from Cirsium setosum.
[So] Source:J Asian Nat Prod Res;18(11):1015-23, 2016 Nov.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Four new taraxastane-type triterpenoids acids 3ß,22α-dihydroxy-20-taraxasten-30-oic acid (1), 3ß-hydroxy-22-oxo-20-taraxasten-30-oic acid (2), 3-oxo-22α-hydroxy-20- taraxasten-30-oic acid (3), and 3ß,19ß-dihydroxy-20-taraxasten-30-oic acid (4) were isolated and characterized from Cirsium setosum (Willd.) MB. Their structures were determined by the combination of 1D and 2D NMR experiments ((1)H-(1)HCOSY, HSQC, HMBC and ROESY) and mass spectrometry. Compound 2 exhibited potent selective cytotoxicity against human ovarian cancer cell line A2780 with an IC50 value of 3.9 µM.
[Mh] Termos MeSH primário: Cirsium/química
Triterpenos/isolamento & purificação
[Mh] Termos MeSH secundário: Medicamentos de Ervas Chinesas/química
Seres Humanos
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Triterpenos/química
Triterpenos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Triterpenes)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170125
[Lr] Data última revisão:
170125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160907
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2016.1217519


  7 / 140 MEDLINE  
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[PMID]:27490443
[Au] Autor:Aysu T
[Ad] Endereço:Yüzüncü Yil University, Faculty of Science, Department of Chemistry, Van, Turkey. Electronic address: tevfikaysu@yyu.edu.tr.
[Ti] Título:A study on pyrolysis of Canada thistle (Cirsium arvense) with titania based catalysts for bio-fuel production.
[So] Source:Bioresour Technol;219:175-184, 2016 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The catalytic pyrolysis of Cirsium arvense was performed with titania supported catalysts under the operating conditions of 500°C, 40°C/min heating rate, 100mL/min N2 flow rate in a fixed bed reactor for biofuel production. The effect of catalysts on product yields was investigated. The amount of pyrolysis products (bio-char, bio-oil, gas) and the composition of the produced bio-oils were determined by proton nuclear magnetic resonance ((1)H NMR), Fourier transform infrared spectroscopy (FT-IR), gas chromatography/mass spectrometry (GC-MS) and elemental analysis (EA) techniques. Thistle bio-oils had lower O/C and H/C molar ratios compared to feedstock. The highest bio-char and bio-oil yields of 29.32wt% and 36.71wt% were obtained in the presence of Ce/TiO2 and Ni/TiO2 catalysts respectively. GC-MS identified 97 different compounds in the bio-oils obtained from thistle pyrolysis. (1)H NMR analysis showed that the bio-oils contained ∼55-77% aliphatic and ∼6-19% aromatic structural units.
[Mh] Termos MeSH primário: Biocombustíveis
Cirsium/química
[Mh] Termos MeSH secundário: Biocombustíveis/análise
Canadá
Catálise
Cromatografia Gasosa-Espectrometria de Massas
Temperatura Alta
Espectroscopia de Ressonância Magnética
Óleos/química
Espectroscopia de Infravermelho com Transformada de Fourier
Titânio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Oils); 15FIX9V2JP (titanium dioxide); D1JT611TNE (Titanium)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170813
[Lr] Data última revisão:
170813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160805
[St] Status:MEDLINE


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[PMID]:27308841
[Au] Autor:Shang DL; Ma QG; Wei RR
[Ad] Endereço:a Department of Cardiology , Nanyang the First People's Hospital , Nanyang 473002 , China.
[Ti] Título:Cytotoxic phenylpropanoid glycosides from Cirsium japonicum.
[So] Source:J Asian Nat Prod Res;18(12):1122-1130, 2016 Dec.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three new phenylpropanoid glycosides 1-3, along with nine known phenylpropanoid glycosides 4-12, were isolated from the aerial parts of Cirsium japonicum. The structures of isolated compounds were elucidated by chemical and spectroscopic methods. Compounds 1, 3, 6, 8, and 11 showed moderate cytotoxicities against MCF-7, U87, HCT116, and A549 cell lines with IC values in the range of 1.35-11.32 µM. The known compounds 4-12 were obtained from this plant for the first time.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacologia
Cirsium/química
Glicosídeos/isolamento & purificação
Glicosídeos/farmacologia
Fenilpropionatos/isolamento & purificação
Fenilpropionatos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Ensaios de Seleção de Medicamentos Antitumorais
Feminino
Glicosídeos/química
Células HCT116
Seres Humanos
Concentração Inibidora 50
Estrutura Molecular
Fenilpropionatos/química
Componentes Aéreos da Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Glycosides); 0 (Phenylpropionates)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160617
[St] Status:MEDLINE


  9 / 140 MEDLINE  
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[PMID]:27118077
[Au] Autor:Bhatia RK; Bhatia SK; Mehta PK; Bhalla TC
[Ad] Endereço:Department of Biotechnology, Himachal Pradesh University.
[Ti] Título:Bio-statistical enhancement of acyl transfer activity of amidase for biotransformation of N-substituted aromatic amides.
[So] Source:J Gen Appl Microbiol;62(2):90-7, 2016.
[Is] ISSN:1349-8037
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Acyl transfer activity (ATA) of amidase transfers an acyl group of different amides to hydroxylamine to form the corresponding hydroxamic acid. Bacterial isolate BR-1 was isolated from cyanogenic plant Cirsium vulgare rhizosphere and identified as Pseudomonas putida BR-1 by 16S rDNA sequencing. This organism exhibited high ATA for the biotransformation of N-substituted aromatic amide to the corresponding hydroxamic acid. Optimization of media, tryptone (0.6%), inducer, pH 8.5, and a growth temperature 25°C for 56 h, resulted in a 7-fold increase in ATA. Further, Response Surface Methodology (RSM) and multiple feeding approach (20 mM after 14 h) of inducer led to a 29% enhancement of ATA from this organism. The half life (t1/2) of this enzyme at 50°C and 60°C was 3 h and 1 h, respectively. The ATA of amidase of Pseudomonas putida BR-1 makes it a potential candidate for the production of a variety of N-substituted aromatic hydroxamic acid.
[Mh] Termos MeSH primário: Amidas/metabolismo
Amidoidrolases/metabolismo
Biotransformação
Ácidos Hidroxâmicos/metabolismo
Hidroxilamina/metabolismo
Pseudomonas putida/enzimologia
[Mh] Termos MeSH secundário: Amidoidrolases/química
Cirsium/microbiologia
Meios de Cultura/química
Meia-Vida
Concentração de Íons de Hidrogênio
Hidroxilamina/química
Pseudomonas putida/genética
Pseudomonas putida/isolamento & purificação
Pseudomonas putida/metabolismo
Rizosfera
Especificidade por Substrato
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amides); 0 (Culture Media); 0 (Hydroxamic Acids); 2FP81O2L9Z (Hydroxylamine); EC 3.5.- (Amidohydrolases); EC 3.5.1.4 (amidase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160428
[St] Status:MEDLINE
[do] DOI:10.2323/jgam.62.90


  10 / 140 MEDLINE  
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[PMID]:27064010
[Au] Autor:Inafuku M; Takara K; Taira N; Nugara RN; Kamiyama Y; Oku H
[Ad] Endereço:Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Okinawa 903-0213, Japan.
[Ti] Título:Monogalactosyldiacylglycerol: An abundant galactosyllipid of Cirsium brevicaule A. GRAY leaves inhibits the expression of gene encoding fatty acid synthase.
[So] Source:Phytomedicine;23(5):509-16, 2016 May 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The leaves of Cirsium brevicaule A. GRAY (CL) significantly decreased hepatic lipid accumulation and the expression of fatty acid synthase gene (FASN) in mice. PURPOSE: We aimed to purify and identify the active compound(s) from CL and determine the inhibitory mechanism of expression of FASN. METHODS: We purified monogalactosyldiacylglycerol (MGDG) from extracts of CL (CL-MGDG) and showed that it was the active CL component through analyses of its effects on the expression of genes of human breast cancer cell line, SKBR-3. RESULTS: The content and fatty acid composition of CL-MGDG are distinctly different from those of other vegetable-derived MGDGs. Treatment of SKBR-3 cells with MGDG decreased the level of FASN mRNA as well as the levels of mRNA encoding other protein involved in lipogenesis. Further, MGDG treatments significantly inhibited luciferase activities of constructs containing liver X receptor response element in FASN promoter region without altering the levels of mRNA encoding transcription factors. MGDG and the FASN inhibitor C75 decreased the viabilities of SKBR-3 cells in a concentration-dependent manner. CL-MGDG more potently inhibited cell viability than a commercial MGDG preparation. CONCLUSIONS: CL represents a good source of glycoglycerolipids with potential as functional ingredients of food.
[Mh] Termos MeSH primário: Cirsium/química
Ácido Graxo Sintase Tipo I/metabolismo
Galactolipídeos/química
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Ácido Graxo Sintase Tipo I/genética
Ácidos Graxos/química
Seres Humanos
Lipogênese
Fígado/metabolismo
Estrutura Molecular
RNA Mensageiro
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Galactolipids); 0 (RNA, Messenger); 0 (monogalactosyldiacylglycerol); EC 2.3.1.85 (FASN protein, human); EC 2.3.1.85 (Fatty Acid Synthase, Type I)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160412
[St] Status:MEDLINE



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