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Pesquisa : B01.650.940.800.575.912.250.100.289 [Categoria DeCS]
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  1 / 37 MEDLINE  
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[PMID]:28129432
[Au] Autor:Asano S; Hirayama Y; Matsushita Y
[Ad] Endereço:Nara Prefecture Agricultural Research and Development Center, Sakurai, Japan.
[Ti] Título:Distribution of Tomato spotted wilt virus in dahlia plants.
[So] Source:Lett Appl Microbiol;64(4):297-303, 2017 Apr.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tomato spotted wilt virus (TSWV) causes significant losses in the production of the ornamental plant Dahlia variabilis in Japan. The purpose of this study was to examine the distribution of TSWV in dahlia plants and identify plant parts that can be used in the selection of TSWV-free plants. The distribution of TSWV was investigated using reverse transcriptional polymerase chain reaction (RT-PCR) and tissue blot immunoassay. The detection rate of TSWV in latent infected compound leaves was the highest in the petiole, and it decreased from the veins and rachis to the lamina. The tissue blot immunoassays of the leaflets showed an uneven distribution of TSWV, especially along the edge of the leaf blade. In stems, the detection rate of TSWV was high partway up the stem compared to that in the upper and the lower parts of the stem during the vegetative growth stage. A highly uneven distribution was observed in the bulb. Our results indicated that middle parts of the stem as well as the petioles, rachis, and veins of compound leaves are suitable for detection of TSWV in dahlias. This study is the first to report uneven distribution of TSWV in dahlia plants. SIGNIFICANCE AND IMPACT OF THE STUDY: In this study, the distribution of Tomato spotted wilt virus (TSWV) in various parts of dahlia plants was investigated for the first time. The distribution of TSWV was uneven in compound leaves, leaflets, stems, and bulbs. The middle parts of the stem or the petiole and leaf veins should be sampled to detect TSWV when selecting healthy plants.
[Mh] Termos MeSH primário: Dahlia/virologia
Doenças das Plantas/virologia
Tospovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Japão
Folhas de Planta/virologia
Raízes de Plantas/virologia
Caules de Planta/virologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tospovirus/genética
Tospovirus/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12720


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[PMID]:28069127
[Au] Autor:Aghagoli MJ; Hossein Beyki M; Shemirani F
[Ad] Endereço:School of Chemistry, University College of Science, University of Tehran, P.O. Box 14155-6455, Tehran, Iran.
[Ti] Título:Application of dahlia-like molybdenum disulfide nanosheets for solid phase extraction of Co(II) in vegetable and water samples.
[So] Source:Food Chem;223:8-15, 2017 May 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recently, molybdenum disulfide has been attracted considerable attention due to its unique three layered structure. Adsorption sites with abundant electron density on edges and surfaces of MoS can adsorb different metal ions with no need to ligand and functionalization. In this study, dahlia-like MoS nanosheets were prepared by a simple hydrothermal method and characterized using different tools such as FESEM, TEM, EDX, XRD, DLS and zeta potential measurements. Then, they were applied for solid phase extraction of Co(II) as an example of heavy metals. Different factors (the pH, adsorbent amount, contact time, type of eluent, matrix and reusability) affecting the extraction process were studied. Under optimum conditions, the relative standard deviation, adsorption capacity and limit of detection were 2.3%, 80.0mgg and 0.31µgL , respectively. The accuracy of the method was confirmed by analyzing the standard reference material (SRM 1640a) and spiked real samples.
[Mh] Termos MeSH primário: Cobalto/análise
Dahlia
Dissulfetos/química
Molibdênio/química
Nanoestruturas/química
Verduras/química
Água/análise
[Mh] Termos MeSH secundário: Metais Pesados/análise
Extratos Vegetais/análise
Extração em Fase Sólida/métodos
Espectrofotometria Atômica/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disulfides); 0 (Metals, Heavy); 0 (Plant Extracts); 059QF0KO0R (Water); 3G0H8C9362 (Cobalt); 81AH48963U (Molybdenum); ZC8B4P503V (molybdenum disulfide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE


  3 / 37 MEDLINE  
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[PMID]:27855909
[Au] Autor:Pires TC; Dias MI; Barros L; Ferreira IC
[Ad] Endereço:Mountain Research Center (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 1172, 5300-253 Bragança, Portugal.
[Ti] Título:Nutritional and chemical characterization of edible petals and corresponding infusions: Valorization as new food ingredients.
[So] Source:Food Chem;220:337-343, 2017 Apr 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Edible flowers provide new colours, textures and vibrancy to any dish, and apart from the "glam" factor, they can constitute new sources of bioactive compounds. In the present work, the edible petals and infusions of dahlia, rose, calendula and centaurea, were characterized regarding their nutritional value and composition in terms of hydrophilic and lipophilic compounds. Carbohydrates were the most abundant macronutrients, followed by proteins and ash. Fructose, glucose and sucrose were identified in all the petals and infusions. Rose petals and calendula infusions gave the highest content of organic acids, mainly due to the presence of malic and quinic acids, respectively. Polyunsaturated fatty acids predominated over saturated fatty acids, mainly due to the contribution of linoleic acid. Calendula presented the highest content in tocopherols, with α-tocopherol as the most abundant. These results highlight the interest of edible petals "as" and "in" new food products, representing rich sources of bioactive nutrients.
[Mh] Termos MeSH primário: Análise de Alimentos
Tocoferóis/análise
[Mh] Termos MeSH secundário: Calendula/química
Carboidratos/análise
Dahlia/química
Ácidos Graxos/análise
Ácidos Graxos Insaturados/análise
Ácido Linoleico/análise
Valor Nutritivo
Rosa/química
alfa-Tocoferol/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Fatty Acids); 0 (Fatty Acids, Unsaturated); 9KJL21T0QJ (Linoleic Acid); H4N855PNZ1 (alpha-Tocopherol); R0ZB2556P8 (Tocopherols)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


  4 / 37 MEDLINE  
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[PMID]:26732488
[Au] Autor:Tsushima D; Tsushima T; Sano T
[Ad] Endereço:Department of Bio-resources, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036-8561, Japan; Union Graduate school of Agricultural Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
[Ti] Título:Molecular dissection of a dahlia isolate of potato spindle tuber viroid inciting a mild symptoms in tomato.
[So] Source:Virus Res;214:11-8, 2016 Mar 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The dahlia isolate of potato spindle tuber viroid (PSTVd) accumulates slowly and induces mild disease symptoms in tomato (Solanum lycopersicum, cv. Rutgers) plants in contrast to the intermediate isolate (PSTVd-I). The dahlia isolate (PSTVd-D) differs from PSTVd-I in eight locations: 42 and 43 in the terminal left (TL); 64/65, 311, and 312/313 in the pathogenicity (P); 118 and 126 in the variable (V); and 201 in the terminal right (TR) domains. To investigate the molecular determinants in the PSTVd-D genome responsible for the attenuation of symptom severity and lower replication/accumulation in tomato plants, a series of mutants between PSTVd-D and PSTVd-I were constructed by focusing first on the mutations in the TL and P domains in the left-hand half of the molecule. Then, more detailed analysis was performed on the three mutations at positions 118, 126, and 201 in the V and TR domains. One of these mutations is located around the boundary of the right border of the RY-motif, a predicted recognition site of Virp1, a viroid-binding protein. Of 14 mutants (seven based on PSTVd-D and the other seven based on PSTVd-I) examined, 11 propagated stably and three lost infectivity. Mutations in the TL and P domains (42U, 43C, 310U/C, and U or UU insertion to 311/312 in PSTVd mild types) majorly influenced the expression of mild-like symptoms. In contrast, when each of the mutations at 118, 126, and 201 in the V and TR domains were exchanged independently, they minimally influenced systemic accumulation and symptom expression. Mutants based on PSTVd-D with PSTVd-I-type mutations at nucleotide positions 118, 126, and/or 201 showed mild symptoms similar to PSTVd-D, but their systemic accumulation was a little faster than PSTVd-D. In contrast, mutants based on PSTVd-I with PSTVd-D-type mutations at 118, 126, and/or 201 nucleotide positions showed severe symptoms similar to PSTVd-I, and the systemic accumulation was similar to or a little slower than PSTVd-I. The nucleotide at position 201 could be changed to U, G, or A, but C was not acceptable for replication. Because introduction of C at the position 201 can change the loop structure at the right boundary of the RY-motif's consensus sequence, the loop structure may influence recognition by Virp1.
[Mh] Termos MeSH primário: Dahlia/virologia
Doenças das Plantas/virologia
Viroides/genética
Viroides/isolamento & purificação
[Mh] Termos MeSH secundário: Análise Mutacional de DNA
DNA Viral
Genoma Viral
Instabilidade Genômica
Lycopersicon esculentum/virologia
Mutação
Conformação de Ácido Nucleico
Fenótipo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160107
[St] Status:MEDLINE


  5 / 37 MEDLINE  
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[PMID]:26426420
[Au] Autor:Xu C; Yu M; Noonan O; Zhang J; Song H; Zhang H; Lei C; Niu Y; Huang X; Yang Y; Yu C
[Ad] Endereço:Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
[Ti] Título:Core-Cone Structured Monodispersed Mesoporous Silica Nanoparticles with Ultra-large Cavity for Protein Delivery.
[So] Source:Small;11(44):5949-55, 2015 Nov 25.
[Is] ISSN:1613-6829
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A new type of monodispersed mesoporous silica nanoparticles with a core-cone structure (MSN-CC) has been synthesized. The large cone-shaped pores are formed by silica lamellae closely packed encircling a spherical core, showing a structure similar to the flower dahlia. MSN-CC has a large pore size of 45 nm and a high pore volume of 2.59 cm(3) g(-1). MSN-CC demonstrates a high loading capacity of large proteins and successfully delivers active ß-galactosidase into cells, showing their potential as efficient nanocarriers for the cellular delivery of proteins with large molecular weights.
[Mh] Termos MeSH primário: Sistemas de Liberação de Medicamentos/métodos
Nanopartículas/química
Dióxido de Silício/química
beta-Galactosidase/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Dahlia/anatomia & histologia
Camundongos
Nanopartículas/ultraestrutura
Porosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
7631-86-9 (Silicon Dioxide); EC 3.2.1.23 (beta-Galactosidase)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151002
[St] Status:MEDLINE
[do] DOI:10.1002/smll.201501449


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[PMID]:26186968
[Au] Autor:Deguchi A; Tatsuzawa F; Hosokawa M; Doi M; Ohno S
[Ad] Endereço:Laboratory of Vegetable and Ornamental Horticulture, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
[Ti] Título:Tobacco streak virus (strain dahlia) suppresses post-transcriptional gene silencing of flavone synthase II in black dahlia cultivars and causes a drastic flower color change.
[So] Source:Planta;242(3):663-75, 2015 Sep.
[Is] ISSN:1432-2048
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:MAIN CONCLUSION: Tobacco streak virus suppressed post-transcriptional gene silencing and caused a flower color change in black dahlias, which supported the role of cyanidin-based anthocyanins for black flower appearance. Black flower color of dahlia (Dahlia variabilis) has been attributed, in part, to the high accumulation of cyanidin-based anthocyanins that occurs when flavone synthesis is reduced because of post-transcriptional gene silencing (PTGS) of flavone synthase II (DvFNS). There are also purple-flowering plants that have emerged from a black cultivar 'Kokucho'. We report that the purple color is not caused by a mutation, as previously thought, but by infection with tobacco streak virus (TSVdahlia), which suppresses the PTGS of DvFNS. When TSVdahlia was eliminated from the purple-flowering 'Kokucho' by leaf primordia-free shoot apical meristem culture, the resulting flowers were black. TSVdahlia-infected purple flowers had lower numbers of siRNAs to DvFNS than black flowers, suggesting that TSVdahlia has a silencing suppressor. The graft inoculation of other black cultivars with TSVdahlia altered their flower color drastically except for 'Fidalgo Blacky', a very deep black cultivar with the highest amount of cyanidin-based anthocyanins. The flowers of all six TSVdahlia-infected cultivars accumulated increased amounts of flavones and reduced amounts of cyanidin-based anthocyanins. 'Fidalgo Blacky' remained black despite the change in pigment accumulation, and the amounts of cyanidin-based anthocyanins in its TSVdahlia-infected plants were still higher than those of other cultivars. We propose that black flower color in dahlia is controlled by two different mechanisms that increase the amount of cyanidin-based anthocyanins: DvFNS PTGS-dependent and -independent mechanisms. If both mechanisms occur simultaneously, the flower color will be blacker than if only a single mechanism is active.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/metabolismo
Dahlia/metabolismo
Flores/metabolismo
Ilarvirus/patogenicidade
Pigmentação/fisiologia
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Sistema Enzimático do Citocromo P-450/genética
Dahlia/genética
Dahlia/virologia
Flores/genética
Flores/virologia
Regulação da Expressão Gênica de Plantas
Pigmentação/genética
Proteínas de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.- (cytochrome P-450 CYP93B1 (Glycyrrhiza echinata))
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150719
[St] Status:MEDLINE
[do] DOI:10.1007/s00425-015-2365-6


  7 / 37 MEDLINE  
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[PMID]:25976592
[Au] Autor:Asano S; Matsushita Y; Hirayama Y; Naka T
[Ad] Endereço:Nara Prefectural Agricultural Research and Development Center, Kashihara, Nara, Japan.
[Ti] Título:Simultaneous detection of Tomato spotted wilt virus, Dahlia mosaic virus and Chrysanthemum stunt viroid by multiplex RT-PCR in dahlias and their distribution in Japanese dahlias.
[So] Source:Lett Appl Microbiol;61(2):113-20, 2015 Aug.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Tomato spotted wilt virus (TSWV), Dahlia mosaic virus (DMV) and Chrysanthemum stunt viroid (CSVd) are economically important viruses and viroid that infect cultivated dahlias. Prior to this investigation, no multiplex RT-PCR assay for the detection of dahlia virus and viroid infections existed. In this study, we report the development of a multiplex RT-PCR that simultaneously detects TSWV, DMV and CSVd infections in dahlias. In addition, a simple RT-PCR method that does not require RNA extraction, microtissue direct RT-PCR, could be used to prepare samples for analysis by this multiplex RT-PCR. A field survey validated our results, indicating that TSWV was the dominant virus found in the Kansai region, DMV in the Tohoku and Kyushu regions, and CSVd in the Hokkaido region. This method represents a rapid, sensitive and cost effective approach to diagnose viral infections in dahlias. SIGNIFICANCE AND IMPACT OF THE STUDY: The multiplex RT-PCR assay described in this study is the first report of simultaneous detection of virus and viroid in dahlia. This method represents a rapid, sensitive and cost effective approach to diagnose viral infections in dahlias. A field survey validated our results, indicating that TSWV was the dominant virus found in the Kansai region, DMV in the Tohoku and Kyushu regions and CSVd in the Hokkaido region.
[Mh] Termos MeSH primário: Dahlia/virologia
Vírus do Mosaico/isolamento & purificação
Reação em Cadeia da Polimerase Multiplex/métodos
Doenças das Plantas/virologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Tospovirus/isolamento & purificação
Viroides/isolamento & purificação
[Mh] Termos MeSH secundário: Chrysanthemum/virologia
Dados de Sequência Molecular
Vírus do Mosaico/genética
Tospovirus/genética
Viroides/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150716
[Lr] Data última revisão:
150716
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150516
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12442


  8 / 37 MEDLINE  
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[PMID]:25947569
[Au] Autor:Almeyda CV; Raikhy G; Pappu HR
[Ad] Endereço:Department of Plant Pathology, Washington State University, Pullman, WA, 99163, USA.
[Ti] Título:Characterization and comparative analysis of promoters from three plant pararetroviruses associated with Dahlia (Dahlia variabilis).
[So] Source:Virus Genes;51(1):96-104, 2015 Aug.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two distinct caulimoviruses, Dahlia mosaic virus (DMV) and Dahlia common mosaic virus (DCMV), and an endogenous plant pararetroviral sequence (DvEPRS, formerly known as DMV-D10) were reported from dahlia (Dahlia spp). Promoter elements from these dahlia-associated pararetroviruses were identified and characterized. The TATA box, the CAAT box, the transcription start site, the polyadenylation signal, and regulation factors, characteristic of caulimovirus promoters, were present in each of these promoter regions. Each of the promoter regions was separately cloned into a binary vector containing ß-glucuronidase (GUS) reporter gene and delivered into Agrobacterium tumefaciens by electroporation followed by agroinfiltration into Nicotiana benthamiana. The activity of the 35S promoter homologs was determined by transient expression of the GUS gene both in qualitative and quantitative assays. The length of the promoter regions in DMV, DCMV, and DvEPRS corresponded to 438, 439, and 259 bp, respectively. Quantitative GUS assays showed that the promoters from DMV and DCMV resulted in higher levels of gene expression compared to that of DvEPRS in N. benthamiana leaf tissue. Significant differences were observed among the three promoters (p < 0.001). Qualitative GUS assays were consistent with quantitative GUS results. This study provides important information on new promoters for prospect applications as novel promoters for their potential use in foreign gene expression in plants.
[Mh] Termos MeSH primário: Caulimovirus/genética
Dahlia/virologia
Retrovirus Endógenos/genética
Regiões Promotoras Genéticas
[Mh] Termos MeSH secundário: Fusão Gênica Artificial
Caulimovirus/isolamento & purificação
Clonagem Molecular
Eletroporação
Retrovirus Endógenos/isolamento & purificação
Perfilação da Expressão Gênica
Genes Reporter
Vetores Genéticos
Glucuronidase/análise
Glucuronidase/genética
Elementos Reguladores de Transcrição
Tabaco/virologia
Sítio de Iniciação de Transcrição
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.2.1.31 (Glucuronidase)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150508
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-015-1196-7


  9 / 37 MEDLINE  
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[PMID]:25563135
[Au] Autor:Wang DC; Qiu DR; Shi LN; Pan HY; Li YW; Sun JZ; Xue YJ; Wei DS; Li X; Zhang YM; Qin JC
[Ad] Endereço:a College of Plant Science, Jilin University , Changchun , Jilin 130062 , P.R. China.
[Ti] Título:Identification of insecticidal constituents of the essential oils of Dahlia pinnata Cav. against Sitophilus zeamais and Sitophilus oryzae.
[So] Source:Nat Prod Res;29(18):1748-51, 2015.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this research was to determine the chemical composition of the essential oils of Dahlia pinnata, their insecticidal activity against Sitophilus zeamais and Sitophilusoryzae and to isolate insecticidal constituents. Based on bioactivity-guided fractionation, active constituents were isolated and identified as D-limonene, 4-terpineol and α-terpineol. Essential oils and active compounds tested exhibited contact toxicity, with LD50 values ranging from 132.48 to 828.79 µg/cm(2) against S. zeamais and S. oryzae. Essential oils possessed fumigant toxicity against S. zeamais and S. oryzae with LC50 from 14.10 to 73.46 mg/L. d-Limonene (LC50 = 4.55 and 7.92 mg/L) showed stronger fumigant toxicity against target insects. 4-Terpineol (88 ± 8%) and d-limonene (87 ± 5%) showed the strongest repellency against S. zeamais and S. oryzae, respectively. The results indicate that essential oils and insecticidal constituents have potential for development into natural fumigants, insecticides or repellents for control of the stored-product insect pests.
[Mh] Termos MeSH primário: Dahlia/química
Inseticidas/química
Óleos Voláteis/química
Óleos Vegetais/química
Gorgulhos
[Mh] Termos MeSH secundário: Animais
Cicloexenos/química
Cicloexenos/isolamento & purificação
Mentol/análogos & derivados
Mentol/química
Mentol/isolamento & purificação
Monoterpenos/química
Monoterpenos/isolamento & purificação
Terpenos/química
Terpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (4-methyl-1-(1-methylethyl)-3-cyclohexen-1-ol); 0 (Cyclohexenes); 0 (Insecticides); 0 (Monoterpenes); 0 (Oils, Volatile); 0 (Plant Oils); 0 (Terpenes); 1490-04-6 (Menthol); 21334LVV8W (alpha-terpineol); 9MC3I34447 (limonene)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150805
[Lr] Data última revisão:
150805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150108
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2014.998218


  10 / 37 MEDLINE  
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[PMID]:25559021
[Au] Autor:Rawat HK; Ganaie MA; Kango N
[Ad] Endereço:Department of Applied Microbiology and Biotechnology, Dr. Harisingh Gour University, Sagar, M.P., India.
[Ti] Título:Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides.
[So] Source:Antonie Van Leeuwenhoek;107(3):799-811, 2015 Mar.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Owing to applications in the food and nutraceutical industries, inulinases, fructosyltransferases and sucrases have gained considerable attention in recent times. Twenty-five fungal strains were screened for production of these enzymes on three different media formulated using inulin-rich plant extracts prepared from asparagus root, dahlia tuber and dandelion root extract. Culture filtrates of the fungi were examined for hydrolytic activities. Fungi belonging to genus Aspergillus, A. niger GNCC 2655 (11.3 U/ml), A. awamori MTCC 2879 (8.2 U/ml), A. niger ATCC 26011 (7.9 U/ml) secreted high titers of inulinase followed by Penicillium sp. NFCCI 2768 (2.6 U/ml) and Penicillium citrinum MTCC 1256 (1.1 U/ml). High sucrase activity was noticed in A. niger GNCC 2613 (113 U/ml) and A. awamori MTCC 2879 (107.8 U/ml). Analysis of end products of inulinase action by HPLC revealed that most of the enzymes were exo-inulinases liberating fructose exclusively from inulin. Five fungi, P. citrinum MTCC 1256, Penicillium rugulosum MTCC 3487, Penicillium sp. NFCCI 2768, A. fumigatus GNCC 1351 and A. niger ATCC 26011 however, produced a mixture of endo- and exo-inulinases liberating oligosaccharides (GF3 and GF2) along with fructose. High inulinase/sucrase yielding strains were evaluated for extracellular and intracellular hydrolytic and transfructosylating activities and intracellular enzyme profiles were found to be considerably different in terms of titers and end products.
[Mh] Termos MeSH primário: Frutose/metabolismo
Fungos/metabolismo
Glicosídeo Hidrolases/metabolismo
Hexosiltransferases/metabolismo
Inulina/metabolismo
Oligossacarídeos/metabolismo
Sacarase/metabolismo
[Mh] Termos MeSH secundário: Asparagus (Planta)/química
Cromatografia Líquida de Alta Pressão
Meios de Cultura/química
Dahlia/química
Fungos/classificação
Fungos/enzimologia
Fungos/crescimento & desenvolvimento
Inulina/isolamento & purificação
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/metabolismo
Raízes de Plantas/química
Taraxacum/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); 0 (Oligosaccharides); 0 (Plant Extracts); 0 (fructooligosaccharide); 30237-26-4 (Fructose); 9005-80-5 (Inulin); EC 2.4.1.- (Hexosyltransferases); EC 2.4.1.9 (inulosucrase); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.48 (Sucrase); EC 3.2.1.7 (inulinase)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150224
[Lr] Data última revisão:
150224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150107
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-014-0373-3



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