Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.100.310 [Categoria DeCS]
Referências encontradas : 696 [refinar]
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[PMID]:28783151
[Au] Autor:Nagao A; Ohara M; Miyauchi K; Yokobori SI; Yamagishi A; Watanabe K; Suzuki T
[Ad] Endereço:Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan.
[Ti] Título:Hydroxylation of a conserved tRNA modification establishes non-universal genetic code in echinoderm mitochondria.
[So] Source:Nat Struct Mol Biol;24(9):778-782, 2017 Sep.
[Is] ISSN:1545-9985
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genetic code is not frozen but still evolving, which can result in the acquisition of 'dialectal' codons that deviate from the universal genetic code. RNA modifications in the anticodon region of tRNAs play a critical role in establishing such non-universal genetic codes. In echinoderm mitochondria, the AAA codon specifies asparagine instead of lysine. By analyzing mitochondrial (mt-) tRNA isolated from the sea urchin (Mesocentrotus nudus), we discovered a novel modified nucleoside, hydroxy-N -threonylcarbamoyladenosine (ht A), 3' adjacent to the anticodon (position 37). Biochemical analysis revealed that ht A37 has the ability to prevent mt-tRNA from misreading AAA as lysine, thereby indicating that hydroxylation of N -threonylcarbamoyladenosine (t A) contributes to the establishment of the non-universal genetic code in echinoderm mitochondria.
[Mh] Termos MeSH primário: Echinacea/genética
Echinacea/metabolismo
Código Genético
Mitocôndrias/metabolismo
Processamento Pós-Transcricional do RNA
RNA de Transferência de Lisina/metabolismo
[Mh] Termos MeSH secundário: Asparagina/metabolismo
Hidroxilação
Lisina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Transfer, Lys); 7006-34-0 (Asparagine); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE
[do] DOI:10.1038/nsmb.3449


  2 / 696 MEDLINE  
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[PMID]:28359985
[Au] Autor:Arslan Burnaz N; Küçük M; Akar Z
[Ad] Endereço:Department of Nutrition and Dietetics, Faculty of Health Sciences, Gümüshane University, 29100 Gümüshane, Turkey. Electronic address: nsbburnaz@gmail.com.
[Ti] Título:An on-line HPLC system for detection of antioxidant compounds in some plant extracts by comparing three different methods.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1052:66-72, 2017 May 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Echinacea (Echinacea purpurea), green tea (Camellia sinensis) and yaylacayi (Thymus praecox OPIZ subsp. grossheimii (Ronniger) Jalas) are herbs used in traditional medicine because of their beneficial health effects. A high performance liquid chromatography (HPLC) post-column method was developed by coupling HPLC with on-line antioxidant assays using diode array (DAD) and ultraviolet (UV) detectors. Thus antioxidant compounds and fifteen phenolic standards were detected in echinacea, green tea and yaylacayi methanol extracts simultaneously. Later on, the developed on-line HPLC coupled system was used to compare three different antioxidant methods, ABTS [2,2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)], DPPH (2,2-diphenyl-1-picrylhydrazyl and FRAP (Ferric reducing antioxidant power). Eight compounds with antioxidant activity were identified as gallic acid, protocatechuic aldehyde, p-OH benzoic acid, chlorogenic acid, caffeic acid, vanillin, ferulic acid, and rosmarinic acid. For validation purposes, LOD (limit of detection) (0.36-14.68µg/mL) and LOQ (limit of quantification) (1.35-48.92µg/mL) values were calculated for six standards. The results showed that while the post-column ABTS and FRAP assays were repeatable, sensitive and supportive of each other, the post-column DPPH assay did not provide reliable results. Also due to the base-line noise and drifts in post-column DPPH assay, identification and quantitation of analytes could not be done accurately.
[Mh] Termos MeSH primário: Antioxidantes/análise
Cromatografia Líquida de Alta Pressão/métodos
Medicamentos de Ervas Chinesas/análise
Fenóis/análise
[Mh] Termos MeSH secundário: Antioxidantes/farmacologia
Benzotiazóis/química
Compostos de Bifenilo/química
Camellia sinensis/química
Medicamentos de Ervas Chinesas/farmacologia
Echinacea/química
Radicais Livres/química
Fenóis/farmacologia
Picratos/química
Ácidos Sulfônicos/química
Thymus (Planta)/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Benzothiazoles); 0 (Biphenyl Compounds); 0 (Drugs, Chinese Herbal); 0 (Free Radicals); 0 (Phenols); 0 (Picrates); 0 (Sulfonic Acids); 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE


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[PMID]:28164789
[Au] Autor:Chiellini C; Maida I; Maggini V; Bosi E; Mocali S; Emiliani G; Perrin E; Firenzuoli F; Mengoni A; Fani R
[Ad] Endereço:Department of Biology, University of Florence, Via Madonna del Piano, 6 I-50019 Sesto Fiorentino, Florence, Italy. Electronic address: chiellini.carolina@gmail.com.
[Ti] Título:Preliminary data on antibacterial activity of Echinacea purpurea-associated bacterial communities against Burkholderia cepacia complex strains, opportunistic pathogens of Cystic Fibrosis patients.
[So] Source:Microbiol Res;196:34-43, 2017 Mar.
[Is] ISSN:1618-0623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Burkholderia cepacia complex bacteria (Bcc) represent a serious threat for immune-compromised patient affected by Cystic Fibrosis (CF) since they are resistant to many substances and to most antibiotics. For this reason, the research of new natural compounds able to inhibit the growth of Bcc strains has raised new interest during the last years. A source of such natural compounds is represented by medicinal plants and, in particular, by bacterial communities associated with these plants able to produce molecules with antimicrobial activity. In this work, a panel of 151 (endophytic) bacteria isolated from three different compartments (rhizospheric soil, roots, and stem/leaves) of the medicinal plant Echinacea purpurea were tested (using the cross-streak method) for their ability to inhibit the growth of 10 Bcc strains. Data obtained revealed that bacteria isolated from the roots of E. purpurea are the most active in the inhibition of Bcc strains, followed by bacteria isolated from the rhizospheric soil, and endophytes from stem/leaf compartment. At the same time, Bcc strains of environmental origin showed a higher resistance toward inhibition than the Bcc strains with clinical (i.e. CF patients) origin. Differences in the inhibition activity of E. purpurea-associated bacteria are mainly linked to the environment -the plant compartment- rather than to their taxonomical position.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias/química
Complexo Burkholderia cepacia/efeitos dos fármacos
Complexo Burkholderia cepacia/fisiologia
Fibrose Cística/microbiologia
Echinacea/microbiologia
[Mh] Termos MeSH secundário: Antibacterianos/isolamento & purificação
Bactérias/classificação
Bactérias/genética
Bactérias/metabolismo
Técnicas de Tipagem Bacteriana
Fibrose Cística/tratamento farmacológico
DNA Bacteriano/análise
Endófitos
Filogenia
Folhas de Planta/microbiologia
Raízes de Plantas/microbiologia
Pseudomonas/química
Pseudomonas/genética
Rizosfera
Análise de Sequência
Microbiologia do Solo
Staphylococcus/química
Staphylococcus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170207
[St] Status:MEDLINE


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[PMID]:28061036
[Au] Autor:Chiou SY; Sung JM; Huang PW; Lin SD
[Ad] Endereço:Department of Food Science and Technology, Hungkuang University , Taichung, Taiwan .
[Ti] Título:Antioxidant, Antidiabetic, and Antihypertensive Properties of Echinacea purpurea Flower Extract and Caffeic Acid Derivatives Using In Vitro Models.
[So] Source:J Med Food;20(2):171-179, 2017 Feb.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The extraction yield, total phenols, caffeic acid derivatives (CAD), and antioxidant properties of 50% ethanolic Echinacea purpurea flower extract were determined. The in vitro inhibitory effects of 50% ethanolic extract and CAD on α-amylase, α-glucosidase, and angiotensin-converting enzyme (ACE) linked with type 2 diabetes were also investigated. The extraction yield, total phenols, and total CAD of the extract were 27.04%, 195.69 mg CAE/g and 78.42 mg/g, respectively. Cichoric acid (56.03 mg/g) was the predominant CAD compound in the extract. The extract exhibited good antioxidant properties. The extract and CAD inhibited α-amylase, α-glucosidase, and ACE activities in a concentration-dependent manner. Among the tested samples, chlorogenic acid, and caffeic acid (IC of 1.71-1.81 mg/mL) had the highest α-amylase inhibitory activity, cichoric acid (IC of 0.28 mg/mL) showed higher α-glucosidase inhibitory activity. Both chlorogenic acid and caffeic acid (IC of 0.11-0.14 mg/mL) demonstrated higher ACE-inhibitory activity. The in vitro results suggest that E. purpurea extract and CAD have good potential for managing hyperglycemia and hypertension. Overall, the data suggest it is a choice for developing antihyperglycemia and antihypertension compounds from field-grown E. purpurea.
[Mh] Termos MeSH primário: Anti-Hipertensivos/química
Antioxidantes/química
Ácidos Cafeicos/química
Echinacea/química
Hipoglicemiantes/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Inibidores Enzimáticos/química
Flores/química
Seres Humanos
Cinética
Peptidil Dipeptidase A/química
Fenóis/química
alfa-Amilases/antagonistas & inibidores
alfa-Amilases/química
alfa-Glucosidases/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antihypertensive Agents); 0 (Antioxidants); 0 (Caffeic Acids); 0 (Enzyme Inhibitors); 0 (Hypoglycemic Agents); 0 (Phenols); 0 (Plant Extracts); EC 3.2.1.1 (alpha-Amylases); EC 3.2.1.20 (alpha-Glucosidases); EC 3.4.15.1 (Peptidyl-Dipeptidase A)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1089/jmf.2016.3790


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[PMID]:27951745
[Au] Autor:Aarland RC; Bañuelos-Hernández AE; Fragoso-Serrano M; Sierra-Palacios ED; Díaz de León-Sánchez F; Pérez-Flores LJ; Rivera-Cabrera F; Mendoza-Espinoza JA
[Ad] Endereço:a Postgraduate Program in Experimental Biology , Universidad Autónoma Metropolitana, Iztapalapa, Ciudad de México , México.
[Ti] Título:Studies on phytochemical, antioxidant, anti-inflammatory, hypoglycaemic and antiproliferative activities of Echinacea purpurea and Echinacea angustifolia extracts.
[So] Source:Pharm Biol;55(1):649-656, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Echinacea (Asteraceae) is used because of its pharmacological properties. However, there are few studies that integrate phytochemical analyses with pharmacological effects. OBJECTIVE: Evaluate the chemical profile and biological activity of hydroalcoholic Echinacea extracts. MATERIALS AND METHODS: Density, dry matter, phenols (Folin-Ciocalteu method), flavonoids (AlCl method), alkylamides (GC-MS analysis), antioxidant capacity (DPPH and ABTS methods), antiproliferative effect (SRB assay), anti-inflammatory effect (paw oedema assay, 11 days/Wistar rats; 0.4 mL/kg) and hypoglycaemic effect (33 days/Wistar rats; 0.4 mL/kg) were determined in three Echinacea extracts which were labelled as A, B and C (A, roots of Echinacea purpurea L. Moench; B, roots, leaves, flowers and seeds of Echinacea purpurea; C, aerial parts and roots of Echinacea purpurea and roots of Echinacea angustifolia DC). RESULTS: Extract C showed higher density (0.97 g/mL), dry matter (0.23 g/mL), phenols (137.5 ± 2.3 mEAG/mL), flavonoids (0.62 ± 0.02 mEQ/mL), and caffeic acid (0.048 mg/L) compared to A and B. A, B presented 11 alkylamides, whereas C presented those 11 and three more. B decreased the oedema (40%) on day 2 similar to indomethacin. A and C showed hypoglycaemic activity similar to glibenclamide. Antiproliferative effect was only detected for C (IC 270 µg/mL; 8171 µg/mL; 9338 µg/mL in HeLa, MCF-7, HCT-15, respectively). DISCUSSION AND CONCLUSION: The difference in the chemical and pharmacological properties among extracts highlights the need to consider strategies and policies for standardization of commercial herbal extracts in order to guarantee the safety and identity of this type of products.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Antioxidantes/farmacologia
Proliferação Celular/efeitos dos fármacos
Diabetes Mellitus Experimental/tratamento farmacológico
Echinacea/química
Edema/prevenção & controle
Hipoglicemiantes/farmacologia
Neoplasias/tratamento farmacológico
Compostos Fitoquímicos/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Aloxano
Animais
Anti-Inflamatórios/isolamento & purificação
Antineoplásicos Fitogênicos/isolamento & purificação
Antioxidantes/isolamento & purificação
Benzotiazóis/química
Biomarcadores/sangue
Compostos de Bifenilo/química
Glicemia/efeitos dos fármacos
Glicemia/metabolismo
Carragenina
Diabetes Mellitus Experimental/sangue
Diabetes Mellitus Experimental/induzido quimicamente
Echinacea/classificação
Edema/induzido quimicamente
Células HeLa
Seres Humanos
Hipoglicemiantes/isolamento & purificação
Células MCF-7
Masculino
Neoplasias/patologia
Compostos Fitoquímicos/isolamento & purificação
Fitoterapia
Picratos/química
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Ratos Wistar
Ácidos Sulfônicos/química
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Benzothiazoles); 0 (Biomarkers); 0 (Biphenyl Compounds); 0 (Blood Glucose); 0 (Hypoglycemic Agents); 0 (Phytochemicals); 0 (Picrates); 0 (Plant Extracts); 0 (Sulfonic Acids); 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid); 6SW5YHA5NG (Alloxan); 9000-07-1 (Carrageenan); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE


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[PMID]:27884784
[Au] Autor:Presta L; Bosi E; Fondi M; Maida I; Perrin E; Miceli E; Maggini V; Bogani P; Firenzuoli F; Di Pilato V; Rossolini GM; Mengoni A; Fani R
[Ad] Endereço:Dept of Biology, University of Florence, Via Madonna del Piano 6, I-50019 Sesto F.no, FI, Italy.
[Ti] Título:Phenotypic and genomic characterization of the antimicrobial producer Rheinheimera sp. EpRS3 isolated from the medicinal plant Echinacea purpurea: insights into its biotechnological relevance.
[So] Source:Res Microbiol;168(3):293-305, 2017 Apr.
[Is] ISSN:1769-7123
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:In recent years, there has been increasing interest in plant microbiota; however, despite medicinal plant relevance, very little is known about their highly complex endophytic communities. In this work, we report on the genomic and phenotypic characterization of the antimicrobial compound producer Rheinheimera sp. EpRS3, a bacterial strain isolated from the rhizospheric soil of the medicinal plant Echinacea purpurea. In particular, EpRS3 is able to inhibit growth of different bacterial pathogens (Bcc, Acinetobacter baumannii, and Klebsiella pneumoniae) which might be related to the presence of gene clusters involved in the biosynthesis of different types of secondary metabolites. The outcomes presented in this work highlight the fact that the strain possesses huge biotechnological potential; indeed, it also shows antimicrobial effects upon well-described multidrug-resistant (MDR) human pathogens, and it affects plant root elongation and morphology, mimicking indole acetic acid (IAA) action.
[Mh] Termos MeSH primário: Antibiose
Echinacea/microbiologia
Gammaproteobacteria/genética
Gammaproteobacteria/fisiologia
Rizosfera
[Mh] Termos MeSH secundário: Acinetobacter baumannii/crescimento & desenvolvimento
Antibacterianos/farmacologia
Biotecnologia
Burkholderia cepacia/crescimento & desenvolvimento
Endófitos/isolamento & purificação
Fosfomicina/farmacologia
Gammaproteobacteria/química
Gammaproteobacteria/isolamento & purificação
Genômica
Ácidos Indolacéticos/metabolismo
Klebsiella pneumoniae/crescimento & desenvolvimento
Fenótipo
Raízes de Plantas/microbiologia
Plantas Medicinais/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Indoleacetic Acids); 2N81MY12TE (Fosfomycin); 6U1S09C61L (indoleacetic acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


  7 / 696 MEDLINE  
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[PMID]:27737573
[Au] Autor:Rondanelli M; Riva A; Morazzoni P; Allegrini P; Faliva MA; Naso M; Miccono A; Peroni G; Degli Agosti I; Perna S
[Ad] Endereço:a Department of Public Health, Experimental and Forensic Medicine, Section of Human Nutri tion and Dietetics, Azienda di Servizialla Persona di Pavia , University of Pavia , Pavia , Italy.
[Ti] Título:The effect and safety of highly standardized Ginger (Zingiber officinale) and Echinacea (Echinacea angustifolia) extract supplementation on inflammation and chronic pain in NSAIDs poor responders. A pilot study in subjects with knee arthrosis.
[So] Source:Nat Prod Res;31(11):1309-1313, 2017 Jun.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The study aimed to evaluate the effect of Zingiber officinale and Echinacea angustifolia extract supplementation (25 mg of ginger and 5 mg of Echinacea) for 30 days on inflammation and chronic pain in knee osteoarthritis (OA). Consecutive nonsteroidal anti-inflammatory-drugs (NSAIDs) poor responders with chronic inflammation and pain due to knee arthrosis were assessed (15 subjects, age: 67.2 ± 7.9, body mass index: 30.6 ± 7.1, men/women:2/13). The primary endpoint was to determine pain improvement from baseline to Day 30 by Tegner Lysholm Knee Scoring. The secondary endpoints were the assessment of Visual Analog Scale for Pain, health-related quality of life, by the ShortForm36 (SF-36), anthropometric parameters, hydration. After supplementation, a significant improvement of 12.27 points was observed for Lysholm scale score (p < 0.05), SF-36 (p < 0.05), and a decrease in -0.52 cm in knee circumference (left) (p < 0.01). This pilot study provides feasibility and safety data for the use of highly standardised ginger and Echinacea extract supplementation in people with knee OA.
[Mh] Termos MeSH primário: Dor Crônica/tratamento farmacológico
Echinacea/química
Gengibre/química
Inflamação/tratamento farmacológico
Osteoartrite do Joelho/tratamento farmacológico
Extratos Vegetais/administração & dosagem
[Mh] Termos MeSH secundário: Idoso
Anti-Inflamatórios não Esteroides/farmacologia
Suplementos Nutricionais
Feminino
Seres Humanos
Masculino
Meia-Idade
Medição da Dor/métodos
Projetos Piloto
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
Qualidade de Vida
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Plant Extracts)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161015
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1236097


  8 / 696 MEDLINE  
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[PMID]:28479944
[Au] Autor:Kaur A; Oberhofer M; Juzumaite M; Raja HA; Gulledge TV; Kao D; Faeth SH; Laster SM; Oberlies NH; Cech NB
[Ad] Endereço:Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, Greensboro, NC 24702, USA.
[Ti] Título:Secondary Metabolites from Fungal Endophytes of Suppress Cytokine Secretion by Macrophage-Type Cells.
[So] Source:Nat Prod Commun;11(8):1143-1146, 2016 Jan.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Botanical extracts of have been widely used for the treatment of upper respiratory infections. We sought to chemically examine fungal endophytes inhabiting , and to identify compounds produced by these endophytes with in vitro cytokine-suppressive activity. Twelve isolates from surface sterilized seeds of were subjected to fractionation and major components were isolated. Sixteen secondary metabolites belonging to different structural classes were identified from these isolates based on NMR and mass spectrometry data. The compounds were tested for their influence on cytokine secretion by murine macrophage-type cells. Alternariol ( ), O-prenylporriolide ( ), porritoxin ( ) -zearalenol ( ), and ( )-zearalenone ( ) inhibited production of TNF-α from RAW 264.7 macrophages stimulated with LPS in the absence of any significant cytotoxicity. This is the first report of a cytokine-suppressive effect for . The results of this study are particularly interesting given that they show the presence of compounds with cytokine-suppressive activity in endophytes from a botanical used to treat inflammation. Future investigations into the role of fungal endophytes in the biological activity of dietary supplements may be warranted.
[Mh] Termos MeSH primário: Endófitos
[Mh] Termos MeSH secundário: Animais
Echinacea
Macrófagos
Camundongos
Extratos Vegetais
Fator de Necrose Tumoral alfa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE


  9 / 696 MEDLINE  
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[PMID]:28096776
[Au] Autor:Jiang W; Li Q; Chen X; Ren Y; Chen R; Wu H; Yang Y
[Ad] Endereço:Genetic Engineering Laboratory, College of Life Sciences, South China Agricultural University, Room 614, Guangzhou, 510642 People's Republic of China.
[Ti] Título:A C-banded karyotype of mitotic chromosomes in diploid purple coneflower ( L.).
[So] Source:Hereditas;153:14, 2016.
[Is] ISSN:1601-5223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Aneuploid ermpglasm is an important resource for genetic studies and identification of individual chromosomes in the cells of the aneuploid is an important step. The karyotype has already been established for purple coneflower ( L.), but due to the high similarity in the morphology of several pairs of chromosomes in this species, it cannot be used to identify individual chromosomes in its own complement. The objectives of this study are to develop and evaluate the Giemsa C-banding technique for the purpose of identifying the individual chromosomes in . RESULTS: The established karyotype with C-bands showed that all the 11 pairs of chromosomes possessed centromeric bands. Telomeric bands appeared most frequently in almost all the chromosomes with only two exceptions, the short arm of the chromosome 9 and the long arm of the chromosome 10. Intercalary bands were found mainly in the long arm of some chromosomes with only two exceptions, the chromosomes 1 and 2 that had intercalary bands on both arms. The chromosome 4 was the only chromosome where intercalary bands were absent. CONCLUSIONS: Chromosomes in could be stained with Giemsa to bear C-bands. By classifying the chromosomes into groups and judging the C-bands, each chromosome could be identified. The methods established in this study might be used for the identification of chromosome constitution in aneuploid created in a breeding program.
[Mh] Termos MeSH primário: Bandeamento Cromossômico
Cromossomos de Plantas
Echinacea/genética
Cariotipagem/métodos
[Mh] Termos MeSH secundário: Cariótipo
Mitose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1186/s41065-016-0016-y


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[PMID]:27497272
[Au] Autor:Rizhsky L; Jin H; Shepard MR; Scott HW; Teitgen AM; Perera MA; Mhaske V; Jose A; Zheng X; Crispin M; Wurtele ES; Jones D; Hur M; Góngora-Castillo E; Buell CR; Minto RE; Nikolau BJ
[Ad] Endereço:The Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA.
[Ti] Título:Integrating metabolomics and transcriptomics data to discover a biocatalyst that can generate the amine precursors for alkamide biosynthesis.
[So] Source:Plant J;88(5):775-793, 2016 Dec.
[Is] ISSN:1365-313X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Echinacea genus is exemplary of over 30 plant families that produce a set of bioactive amides, called alkamides. The Echinacea alkamides may be assembled from two distinct moieties, a branched-chain amine that is acylated with a novel polyunsaturated fatty acid. In this study we identified the potential enzymological source of the amine moiety as a pyridoxal phosphate-dependent decarboxylating enzyme that uses branched-chain amino acids as substrate. This identification was based on a correlative analysis of the transcriptomes and metabolomes of 36 different E. purpurea tissues and organs, which expressed distinct alkamide profiles. Although no correlation was found between the accumulation patterns of the alkamides and their putative metabolic precursors (i.e., fatty acids and branched-chain amino acids), isotope labeling analyses supported the transformation of valine and isoleucine to isobutylamine and 2-methylbutylamine as reactions of alkamide biosynthesis. Sequence homology identified the pyridoxal phosphate-dependent decarboxylase-like proteins in the translated proteome of E. purpurea. These sequences were prioritized for direct characterization by correlating their transcript levels with alkamide accumulation patterns in different organs and tissues, and this multi-pronged approach led to the identification and characterization of a branched-chain amino acid decarboxylase, which would appear to be responsible for generating the amine moieties of naturally occurring alkamides.
[Mh] Termos MeSH primário: Amidas/metabolismo
Echinacea/genética
Echinacea/metabolismo
Metabolômica/métodos
Transcriptoma/genética
[Mh] Termos MeSH secundário: Biocatálise
Ácidos Graxos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amides); 0 (Fatty Acids)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE
[do] DOI:10.1111/tpj.13295



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