[PMID]: | 28951216 |
[Au] Autor: | Wang H; Li N; Zhu C; Shi S; Jin H; Wang S |
[Ad] Endereço: | The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China. |
[Ti] Título: | Anti-complementary activity of two homogeneous polysaccharides from Eclipta prostrata. |
[So] Source: | Biochem Biophys Res Commun;493(2):887-893, 2017 Nov 18. |
[Is] ISSN: | 1090-2104 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Complement-mediated inflammation and tissue damage is an important drive to pathology in autoimmune diseases, targeting inhibit the complement activation is promising treatment strategy for these diseases. We performed anticomplement activity-guided fractionation of the water extract of Eclipta prostrata by ion-exchange and size-exclusion chromatography, yielding two bioactive polysaccharides EAP20-1 and EAP20-2. The molecular weights of EAP20-1 and EAP20-2 were respectively calculated to be 5.2 kDa and 6.3 kDa by HPGPC, both polysaccharides was composed by d-Gal, l-Glc, and Ara at different ratios of 7.3:2.7:1 and 7.6:3.1:1. In addition, the main linkage types of EAP20-1 and EAP20-2 were ß-1,4-Gal, ß-1,6-Gal and α-1,4,6-Glc according to methylation analyses. EAP20-1 and EAP20-2 exhibited significant inhibitory effect on the complement activation through both classical and alternative pathways and with no influence on the coagulation system. Preliminary mechanism study indicated that both EAP20-1 and EAP20-2 inhibited the activation of the complement system by interacting with C1q, C1r, C1s, C2, C4, C5, C7, and C9 components. |
[Mh] Termos MeSH primário: |
Ativação do Complemento/efeitos dos fármacos Proteínas do Sistema Complemento/imunologia Eclipta/química Polissacarídeos/química Polissacarídeos/farmacologia
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[Mh] Termos MeSH secundário: |
Animais Coagulação Sanguínea/efeitos dos fármacos Cromatografia em Gel Polissacarídeos/isolamento & purificação Ovinos
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Polysaccharides); 9007-36-7 (Complement System Proteins) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171020 |
[Lr] Data última revisão:
| 171020 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170928 |
[St] Status: | MEDLINE |
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