Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.100.337 [Categoria DeCS] Referências encontradas : 102 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 29175456 [Au] Autor: Gupta A; Kumar A; Kumar D; Singh R; Shankar K; Varshney S; Rajan S; Srivastava A; Gupta S; Narender T; Gaikwad AN [Ad] Endereço: Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow 226031, India. [Ti] Título: Ecliptal, a promising natural lead isolated from Eclipta alba modulates adipocyte function and ameliorates metabolic syndrome. [So] Source: Toxicol Appl Pharmacol;338:134-147, 2018 01 01. [Is] ISSN: 1096-0333 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A swift increase has been observed in the number of individuals with metabolic syndrome worldwide. A number of natural compounds have been identified towards combating metabolic syndrome. Adding to this premise, here we report the pleiotropic activities of Ecliptal (EC); a natural compound isolated from the herb Eclipta alba. Administration of EC was shown to have prominent anti-adipogenic effects in 3T3-L1 and hMSC derived adipocytes. It was shown to activate Wnt-pathway and alter AKT signaling. Additionally, it caused cell cycle arrest and inhibited mitotic clonal expansion. EC treatment augmented mitochondrial biogenesis as well as function as estimated by expression of PGC1α, UCP-1, mitochondrial complexes and estimation of oxygen consumption rate. EC also reduced LPS-induced inflammation and tunicamycin induced ER stress. Further, EC enhanced insulin sensitivity by increasing AKT phosphorylation, inhibiting PKCα/ßII phosphorylation and reducing leptin/adiponectin ratio. Finally, EC administration in Syrian golden hamsters was shown to have potent anti-dyslipidemic effects. Cumulatively, encompassing pleiotropic activities of EC, it could prove to be a potential drug candidate against obesity, insulin resistance and related metabolic syndrome. [Mh] Termos MeSH primário: Adipócitos/efeitos dos fármacos Eclipta/química Síndrome Metabólica/tratamento farmacológico [Mh] Termos MeSH secundário: Células 3T3-L1 Adipócitos/fisiologia Adipogenia/efeitos dos fármacos Animais Diferenciação Celular/efeitos dos fármacos Estresse do Retículo Endoplasmático/efeitos dos fármacos Masculino Mesocricetus Camundongos Mitocôndrias/efeitos dos fármacos Extratos Vegetais/farmacologia Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores Tiofenos/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Plant Extracts); 0 (Thiophenes); 0 (ecliptal); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180213 [Lr] Data última revisão: 180213 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171128 [St] Status: MEDLINE

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[PMID]: 28951216 [Au] Autor: Wang H; Li N; Zhu C; Shi S; Jin H; Wang S [Ad] Endereço: The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China. [Ti] Título: Anti-complementary activity of two homogeneous polysaccharides from Eclipta prostrata. [So] Source: Biochem Biophys Res Commun;493(2):887-893, 2017 Nov 18. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Complement-mediated inflammation and tissue damage is an important drive to pathology in autoimmune diseases, targeting inhibit the complement activation is promising treatment strategy for these diseases. We performed anticomplement activity-guided fractionation of the water extract of Eclipta prostrata by ion-exchange and size-exclusion chromatography, yielding two bioactive polysaccharides EAP20-1 and EAP20-2. The molecular weights of EAP20-1 and EAP20-2 were respectively calculated to be 5.2 kDa and 6.3 kDa by HPGPC, both polysaccharides was composed by d-Gal, l-Glc, and Ara at different ratios of 7.3:2.7:1 and 7.6:3.1:1. In addition, the main linkage types of EAP20-1 and EAP20-2 were ß-1,4-Gal, ß-1,6-Gal and α-1,4,6-Glc according to methylation analyses. EAP20-1 and EAP20-2 exhibited significant inhibitory effect on the complement activation through both classical and alternative pathways and with no influence on the coagulation system. Preliminary mechanism study indicated that both EAP20-1 and EAP20-2 inhibited the activation of the complement system by interacting with C1q, C1r, C1s, C2, C4, C5, C7, and C9 components. [Mh] Termos MeSH primário: Ativação do Complemento/efeitos dos fármacos Proteínas do Sistema Complemento/imunologia Eclipta/química Polissacarídeos/química Polissacarídeos/farmacologia [Mh] Termos MeSH secundário: Animais Coagulação Sanguínea/efeitos dos fármacos Cromatografia em Gel Polissacarídeos/isolamento & purificação Ovinos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Polysaccharides); 9007-36-7 (Complement System Proteins) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171020 [Lr] Data última revisão: 171020 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170928 [St] Status: MEDLINE

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[PMID]: 28659027 [Au] Autor: Kim DS; Kim SH; Kee JY; Han YH; Park J; Mun JG; Joo MJ; Jeon YD; Kim SJ; Park SH; Park SJ; Um JY; Hong SH [Ad] Endereço: * Department of Oriental Pharmacy, College of Pharmacy, Wonkwnag University, Iksan, Jeonbuk 54538, Republic of Korea. [Ti] Título: Eclipta prostrata Improves DSS-Induced Colitis through Regulation of Inflammatory Response in Intestinal Epithelial Cells. [So] Source: Am J Chin Med;45(5):1047-1060, 2017. [Is] ISSN: 0192-415X [Cp] País de publicação: Singapore [La] Idioma: eng [Ab] Resumo: Eclipta prostrata (EP) and its compounds are known to have several pharmacological effects including anti-inflammatory effects. In the present study, we demonstrated that EP improves the dextran sulfate sodium (DSS)-induced colitis symptoms such as body weight loss, colon length shortening and disease activity index. In DSS-induced colitis tissue, EP controls the protein expressions of cyclooxygenase-2 (COX-2) and hypoxia inducible factor-1[Formula: see text] (HIF-1[Formula: see text]). In addition, the release of prostaglandin E and vascular endothelial growth factor-A were significantly reduced by EP administration. EP also inhibited COX-2 and HIF-1[Formula: see text] expressions in the tumor necrosis factor-[Formula: see text] stimulated HT-29 cells. These inhibitory effects of EP occurred by reducing the phosphorylation of I[Formula: see text]B and the translocation of the nuclear factor-[Formula: see text]B (NF-[Formula: see text]B). Additionally, we found through HPLC analysis that wedelolactone, which is an inhibitor of NF-[Formula: see text]B transcription, was contained in water extract of EP. These results indicate that EP can improve colitis symptoms through the modulation of immune function in intestinal epithelial cells and suggests that EP has the potential therapeutic effect to intestinal inflammation. [Mh] Termos MeSH primário: Anti-Inflamatórios Colite/induzido quimicamente Colite/tratamento farmacológico Sulfato de Dextrana/efeitos adversos Eclipta/química Células Epiteliais/imunologia Células Epiteliais/metabolismo Mediadores da Inflamação/metabolismo Fitoterapia Extratos Vegetais/farmacologia Extratos Vegetais/uso terapêutico [Mh] Termos MeSH secundário: Doença Aguda Animais Células Cultivadas Colite/metabolismo Ciclo-Oxigenase 2/metabolismo Dinoprostona/metabolismo Modelos Animais de Doenças Feminino Células HT29 Seres Humanos Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo Mucosa Intestinal/citologia Mucosa Intestinal/imunologia Mucosa Intestinal/metabolismo Camundongos Endogâmicos BALB C NF-kappa B/metabolismo Índice de Gravidade de Doença Fator de Necrose Tumoral alfa/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Hypoxia-Inducible Factor 1, alpha Subunit); 0 (Inflammation Mediators); 0 (NF-kappa B); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha); 9042-14-2 (Dextran Sulfate); EC 1.14.99.1 (Cyclooxygenase 2); K7Q1JQR04M (Dinoprostone) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170928 [Lr] Data última revisão: 170928 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170630 [St] Status: MEDLINE [do] DOI: 10.1142/S0192415X17500562

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[PMID]: 28506552 [Au] Autor: Preya UH; Lee KT; Kim NJ; Lee JY; Jang DS; Choi JH [Ad] Endereço: Department of Life and Nanopharamceutical Sciences, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, South Korea. [Ti] Título: The natural terthiophene α-terthienylmethanol induces S phase cell cycle arrest of human ovarian cancer cells via the generation of ROS stress. [So] Source: Chem Biol Interact;272:72-79, 2017 Jun 25. [Is] ISSN: 1872-7786 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: Ovarian cancer is the most lethal gynecological malignancy worldwide. Thiophenes such as terthiophene have been shown to have anti-tumor effects on several cancer cell lines, including ovarian cancer cells. However, the underlying mechanisms behind the anti-proliferative effect of thiophenes are poorly understood. In this study, we investigated the molecular mechanisms underlying the anti-proliferative effect of α-terthienylmethanol, a terthiophene isolated from Eclipta prostrata (False Daisy), on human ovarian cancer cells. We found that α-terthienylmethanol is a more potent inhibitor of cell growth than is cisplatin in human ovarian cancer cells. α-Terthienylmethanol induces cell cycle arrest in ovarian cancer cells, as shown by the accumulation of cells in S phase. In addition, α-terthienylmethanol induced a change in S phase-related proteins cyclin A, cyclin-dependent kinase 2, and cyclin D2. Knockdown of cyclin A using specific siRNAs significantly compromised α-terthienylmethanol-induced S phase arrest. We further demonstrated that α-terthienylmethanol induced an increase in intracellular ROS, and the antioxidant N-acetyl-l-cysteine significantly reversed the S phase arrest induced by α-terthienylmethanol. Moreover, α-terthienylmethanol significantly increased the levels of p-H2AX, a DNA damage marker. These results suggest that α-terthienylmethanol inhibits the growth of human ovarian cancer cells by S phase cell cycle arrest via induction of ROS stress and DNA damage. [Mh] Termos MeSH primário: Espécies Reativas de Oxigênio/metabolismo Pontos de Checagem da Fase S do Ciclo Celular/efeitos dos fármacos Tiofenos/toxicidade [Mh] Termos MeSH secundário: Acetilcisteína/farmacologia Apoptose/efeitos dos fármacos Linhagem Celular Tumoral Ciclina A/antagonistas & inibidores Ciclina A/genética Ciclina A/metabolismo Quinase 2 Dependente de Ciclina/metabolismo Dano ao DNA/efeitos dos fármacos Eclipta/química Eclipta/metabolismo Feminino Histonas/metabolismo Seres Humanos Neoplasias Ovarianas/metabolismo Neoplasias Ovarianas/patologia Fosforilação/efeitos dos fármacos Interferência de RNA RNA Interferente Pequeno/metabolismo Tiofenos/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cyclin A); 0 (Histones); 0 (RNA, Small Interfering); 0 (Reactive Oxygen Species); 0 (Thiophenes); 0 (alpha-terthienylmethanol); 0P77RAU2RR (alpha-terthienyl); EC 2.7.11.22 (Cyclin-Dependent Kinase 2); WYQ7N0BPYC (Acetylcysteine) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170620 [Lr] Data última revisão: 170620 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170517 [St] Status: MEDLINE

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[PMID]: 28250894 [Au] Autor: Yadav NK; Arya RK; Dev K; Sharma C; Hossain Z; Meena S; Arya KR; Gayen JR; Datta D; Singh RK [Ad] Endereço: Toxicology Division, CSIR-Central Drug Research Institute, Lucknow 226031, India. [Ti] Título: Alcoholic Extract of Shows Antioxidant and Anticancer Activity without Exhibiting Toxicological Effects. [So] Source: Oxid Med Cell Longev;2017:9094641, 2017. [Is] ISSN: 1942-0994 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: As per WHO estimates, 80% of people around the world use medicinal plants for the cure and prevention of various diseases including cancer owing to their easy availability and cost effectiveness. has long been used in Ayurveda to treat liver diseases, eye ailments, and hair related disorders. The promising medicinal value of prompted us to study the antioxidant, nontoxic, and anticancer potential of its alcoholic extract. In the current study, we evaluated the cytotoxic and antioxidant effect of the alcoholic extract of (AEEA) in multiple cancer cell lines along with control. We have also evaluated its effect on different toxicity parameters. Here, we found that AEEA was found to be most active in most of the cancer cell lines but it significantly induced apoptosis in human breast cancer cell lines by disrupting mitochondrial membrane potential and DNA damage. Moreover, AEEA treatment inhibited migration in both MCF 7 and MDA-MB-231 cells in a dose dependent manner. Further, AEEA possesses robust antioxidant activity along with high total phenolic and flavonoid contents. In summary, our results indicate that has enormous potential in complementary and alternative medicine for the treatment of cancer. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Antioxidantes/farmacologia Eclipta/química Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Animais Antineoplásicos Fitogênicos/efeitos adversos Antioxidantes/efeitos adversos Apoptose/efeitos dos fármacos Células Cultivadas Cercopithecus aethiops Etanol/química Feminino Células HEK293 Células HeLa Seres Humanos Peroxidação de Lipídeos/efeitos dos fármacos Células MCF-7 Masculino Potencial da Membrana Mitocondrial/efeitos dos fármacos Neoplasias/patologia Extratos Vegetais/química Ratos Espécies Reativas de Oxigênio/metabolismo Células Vero [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Plant Extracts); 0 (Reactive Oxygen Species); 3K9958V90M (Ethanol) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170327 [Lr] Data última revisão: 170327 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170303 [St] Status: MEDLINE [do] DOI: 10.1155/2017/9094641

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[PMID]: 27956356 [Au] Autor: Morel LJ; Azevedo BC; Carmona F; Contini SH; Teles AM; Ramalho FS; Bertoni BW; França SC; Borges MC; Pereira AM [Ad] Endereço: Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto, Av. Costábile Romano 2201, 14096-900 Ribeirão Preto, SP, Brazil. [Ti] Título: A standardized methanol extract of Eclipta prostrata (L.) L. (Asteraceae) reduces bronchial hyperresponsiveness and production of Th2 cytokines in a murine model of asthma. [So] Source: J Ethnopharmacol;198:226-234, 2017 Feb 23. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Eclipta prostrata (L.) L. (Asteraceae) has been used in Brazilian traditional medicine to treat asthma and other respiratory illnesses. AIMS OF THE STUDY: To investigate the effects of different doses of a standardized extract of E. prostrata using a murine model of allergen induced asthma. MATERIALS AND METHODS: Balb/c mice were sensitized twice with ovalbumin (OVA) administered intraperitoneally and challenged over four alternate days with nasal instillations of OVA solution. The standardized methanol extract of E. prostrata was administered in doses of 100, 250 and 500mgkg concomitantly with nasal instillation over seven consecutive days. Control animals were treated with dexamethasone or saline solution. Bronchial hyperresponsiveness, production of Th1 and Th2 cytokines, allergen sensitization, airway and lung inflammation, mucous secretion and airway remodeling were assessed. RESULTS: The concentrations of chemical markers in the standardized methanol extract were 0.02% oroboside, 1.69% demethylwedelolactone and 1.71% wedelolactone. Treatment with 250mgkg of extract, which provided 0.745, 4.22 and 4.30mgkg day of oroboside, demethylwedelolactone and wedelolactone, respectively, significantly reduced (P<0.05) respiratory resistance and elastance. Such effects were comparable with those produced by dexamethasone. The total number of inflammatory cells and eosinophils in the bronchoalveolar lavage and the concentrations of interleukin (IL)-4, IL-5 and IL-13 in lung homogenate were significantly reduced (P<0.05) by the methanol extract of E. prostrata. CONCLUSION: The results presented herein demonstrate for the first time the anti-inflammatory activity of E. prostrata in a murine model of asthma, thereby supporting the ethnopharmacological uses of the plant. [Mh] Termos MeSH primário: Asma/tratamento farmacológico Hiper-Reatividade Brônquica/tratamento farmacológico Eclipta/química Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Remodelação das Vias Aéreas/efeitos dos fármacos Alérgenos/imunologia Animais Antiasmáticos/administração & dosagem Antiasmáticos/isolamento & purificação Antiasmáticos/farmacologia Anti-Inflamatórios/administração & dosagem Anti-Inflamatórios/isolamento & purificação Anti-Inflamatórios/farmacologia Asma/imunologia Asma/patologia Brasil Hiper-Reatividade Brônquica/imunologia Hiper-Reatividade Brônquica/patologia Líquido da Lavagem Broncoalveolar/imunologia Citocinas/metabolismo Modelos Animais de Doenças Relação Dose-Resposta a Droga Eosinófilos/metabolismo Masculino Medicina Tradicional/métodos Camundongos Camundongos Endogâmicos BALB C Ovalbumina/imunologia Extratos Vegetais/administração & dosagem Células Th2/imunologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Allergens); 0 (Anti-Asthmatic Agents); 0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Plant Extracts); 9006-59-1 (Ovalbumin) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170703 [Lr] Data última revisão: 170703 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161214 [St] Status: MEDLINE

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[PMID]: 27927066 [Au] Autor: Tambe R; Patil A; Jain P; Sancheti J; Somani G; Sathaye S [Ad] Endereço: a Department of Pharmaceutical Sciences and Technology , Pharmacology Research Lab-II, Institute of Chemical Technology, (University under Section 3 of UGC Act-1956, Elite Status and Centre of Excellence-Government of Maharashtra, TEQIP Phase II Funded) , Mumbai , India. [Ti] Título: Assessment of luteolin isolated from Eclipta alba leaves in animal models of epilepsy. [So] Source: Pharm Biol;55(1):264-268, 2017 Dec. [Is] ISSN: 1744-5116 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: CONTEXT: Eclipta alba (Linn) Hassk. (Asteraceae) has been reported to be a nerve tonic and has been used to treat epilepsy in folk medicine. OBJECTIVE: The present study isolates and characterizes luteolin from E. alba and evaluates its antiepileptic potential in chemically induced acute and chronic models in mice. MATERIALS AND METHODS: The methanol extract (16.85% w/w) of E. alba leaves was subjected to fractionation for isolation of luteolin. In acute pentylenetetrazole (PTZ) model, luteolin (5, 10, 20 mg/kg, i.p.) was administered 30 min prior to PTZ injection (100 mg/kg) in Swiss albino mice. Kindling was induced by chronic administration of PTZ (35 mg/kg) on every alternate day (48 days). Luteolin was investigated on the course of kindling development and oxidative stress markers [reduced glutathione (GSH) and malondialdehyde (MDA)] in kindled mice. RESULTS: Single-dose pretreatment with luteolin (10 and 20 mg/kg, i.p.) was found to be effective in an acute PTZ model (100% protection from mortality) and it did not exhibit any effect on motor coordination at the same doses. PTZ-induced kindling was significantly (p < 0.001) prevented by luteolin (5, 10, 20 mg/kg, i.p.) in a dose-dependent manner. Luteolin restored levels of reduced GSH (p < 0.001) and decreased the level of MDA (p < 0.001), a marker of lipid peroxidation. DISCUSSION AND CONCLUSION: The results of the present study demonstrated that luteolin had an anticonvulsant effect in an acute PTZ model. Luteolin exhibited and inhibitory effect on the course of kindling and associated oxidative stress and hence could be a potential molecule in the treatment of epilepsy. [Mh] Termos MeSH primário: Anticonvulsivantes/farmacologia Encéfalo/efeitos dos fármacos Eclipta/química Luteolina/farmacologia Extratos Vegetais/farmacologia Folhas de Planta/química Convulsões/prevenção & controle [Mh] Termos MeSH secundário: Animais Anticonvulsivantes/isolamento & purificação Biomarcadores/metabolismo Encéfalo/metabolismo Encéfalo/fisiopatologia Modelos Animais de Doenças Relação Dose-Resposta a Droga Glutationa/metabolismo Excitação Neurológica/efeitos dos fármacos Peroxidação de Lipídeos/efeitos dos fármacos Luteolina/isolamento & purificação Malondialdeído/metabolismo Metanol/química Camundongos Atividade Motora/efeitos dos fármacos Estresse Oxidativo/efeitos dos fármacos Pentilenotetrazol Fitoterapia Extratos Vegetais/isolamento & purificação Plantas Medicinais Teste de Desempenho do Rota-Rod Convulsões/induzido quimicamente Convulsões/metabolismo Convulsões/fisiopatologia Solventes/química Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anticonvulsants); 0 (Biomarkers); 0 (Plant Extracts); 0 (Solvents); 4Y8F71G49Q (Malondialdehyde); GAN16C9B8O (Glutathione); KUX1ZNC9J2 (Luteolin); WM5Z385K7T (Pentylenetetrazole); Y4S76JWI15 (Methanol) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170310 [Lr] Data última revisão: 170310 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161209 [St] Status: MEDLINE

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[PMID]: 27902928 [Au] Autor: Xu P; Su S; Tan C; Lai RS; Min ZS [Ad] Endereço: First Clinical College, Nanjing University of Chinese Medicine, Nanjing 210029, China; Department of Dermatology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210009, China. [Ti] Título: Effects of aqueous extracts of Ecliptae herba, Polygoni multiflori radix praeparata and Rehmanniae radix praeparata on melanogenesis and the migration of human melanocytes. [So] Source: J Ethnopharmacol;195:89-95, 2017 Jan 04. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Polygoni multiflori radix praeparata (PMRP), Ecliptae herba (EH) and Rehmanniae radix praeparata (RRP) are the most frequently-used herbs by Traditional Chinese Medicine practitioners for the treatment of vitiligo. Their abilities to stimulate melanogenesis, melanocyte migration and MITF (microphthalmia associated transcription factor) protein expression were evaluated in this study. MATERIALS AND METHODS: The effects of aqueous extracts of PMRP, EH and RRP on human melanocytes in vitro were examined by MTT assay, tyrosinase activity, melanin synthesis, migration assay and Western blot. RESULTS: Treatment with EH (at 100µg/ml and 400µg/ml) significantly increased intracellular tyrosinase activity in accordance with the elevation of melanin content at the same concentrations. Treatment with RRP (at 100µg/ml and 400µg/ml) promoted melanin production but had no stimulatory effect on tyrosinase activity. Treatment with PMRP and EH (at 100µg/ml) promoted the migration of human melanocytes in a type IV collagen-coated transwell migration assay. Western blot analysis showed MITF protein expression was elevated by PMRP, EH and RRP (at 100µg/ml). CONCLUSION: An aqueous extract of EH has a synergistic effect on melanocytes by up-regulating tyrosinase activity, enhancing melanin synthesis and promoting melanocyte migration as well as elevating MITF protein expression. RRP exhibits a significant stimulating effect on melanogenesis and MITF protein expression. These results suggest that EH and RRP contain substances with direct enhancing effects on melanogenesis and migration, possibly via their effects on MITF protein expression. [Mh] Termos MeSH primário: Movimento Celular/efeitos dos fármacos Eclipta/química Melaninas/biossíntese Melanócitos/efeitos dos fármacos Extratos Vegetais/farmacologia Polygonaceae/química Rehmannia/química [Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga Seres Humanos Masculino Melanócitos/metabolismo Fator de Transcrição Associado à Microftalmia/metabolismo Monofenol Mono-Oxigenase/metabolismo Fitoterapia Extratos Vegetais/isolamento & purificação Plantas Medicinais [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (MITF protein, human); 0 (Melanins); 0 (Microphthalmia-Associated Transcription Factor); 0 (Plant Extracts); EC 1.14.18.1 (Monophenol Monooxygenase) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170703 [Lr] Data última revisão: 170703 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161201 [St] Status: MEDLINE

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[PMID]: 27267831 [Au] Autor: Jung WY; Kim H; Park HJ; Jeon SJ; Park HJ; Choi HJ; Kim NJ; Jang DS; Kim DH; Ryu JH [Ad] Endereço: Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea; East-West Medical Research Institute, Kyung Hee University Medical Center, Seoul 130-701, Republic of Korea. [Ti] Título: The ethanolic extract of the Eclipta prostrata L. ameliorates the cognitive impairment in mice induced by scopolamine. [So] Source: J Ethnopharmacol;190:165-73, 2016 Aug 22. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Eclipta prostrata L. (Asteraceae) has been prescribed for whole body nourishment and nervine tonic in Asia. However, the effects of E. prostrata in learning and memory have not been fully explored. AIM OF THE STUDY: To scientifically elucidate the effects of E. prostrata on cognitive functions, we examined whether E. prostrata could ameliorate a cholinergic blockade-induced memory impairment, and we also investigated the effects of E. prostrata on the synaptic plasticity in the hippocampus. MATERIALS AND METHODS: Memory impairment was induced by scopolamine, a cholinergic muscarinic receptor antagonist. The anti-amnesic effects of the ethanolic extract of Eclipta prostrata L. (EEEP) were measured in mice by the passive avoidance, Y-maze and Morris water maze tasks. To test the effects of EEEP on synaptic plasticity, we measured long-term potentiation (LTP) in the hippocampus. We also studied several signaling molecules related to learning and memory, such as phosphorylated protein kinase B (Akt) or phosphorylated glycogen synthase kinase-3ß (GSK-3ß). RESULTS: In the passive avoidance task, EEEP (50 or 100mg/kg, p.o.) significantly ameliorated the shortened step-through latency induced by scopolamine. EEEP (100mg/kg, p.o.) also showed significant increase in alternation behavior during the Y-maze task. In the Morris water maze task, scopolamine-induced a decrease in both the swimming time within the target zone and the number of crossings where the platform had been placed were significantly reversed by EEEP (50 or 100mg/kg, p.o.). Moreover, EEEP (100µg/ml) significantly enhanced hippocampal LTP without affecting basal synaptic transmission. The administration of EEEP (100mg/kg) increased the phosphorylation levels of Akt and GSK-3ß in the hippocampal region. CONCLUSION: These results suggest that EEEP has memory-ameliorating activity against scopolamine-induced cognitive impairment and facilitates LTP in the hippocampus. This could be, at least in part, mediated by the activation of the Akt-GSK-3ß signaling pathway. [Mh] Termos MeSH primário: Amnésia/prevenção & controle Comportamento Animal/efeitos dos fármacos Transtornos Cognitivos/prevenção & controle Cognição/efeitos dos fármacos Eclipta/química Etanol/química Hipocampo/efeitos dos fármacos Memória/efeitos dos fármacos Nootrópicos/farmacologia Extratos Vegetais/farmacologia Hidrobrometo de Escopolamina Solventes/química [Mh] Termos MeSH secundário: Amnésia/induzido quimicamente Amnésia/fisiopatologia Amnésia/psicologia Animais Transtornos Cognitivos/induzido quimicamente Transtornos Cognitivos/fisiopatologia Transtornos Cognitivos/psicologia Modelos Animais de Doenças Relação Dose-Resposta a Droga Glicogênio Sintase Quinase 3 beta/metabolismo Hipocampo/metabolismo Hipocampo/fisiopatologia Potenciação de Longa Duração/efeitos dos fármacos Masculino Aprendizagem em Labirinto/efeitos dos fármacos Camundongos Endogâmicos ICR Atividade Motora/efeitos dos fármacos Nootrópicos/isolamento & purificação Fosforilação Fitoterapia Extratos Vegetais/isolamento & purificação Plantas Medicinais Proteínas Proto-Oncogênicas c-akt/metabolismo Tempo de Reação/efeitos dos fármacos Transdução de Sinais/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Nootropic Agents); 0 (Plant Extracts); 0 (Solvents); 3K9958V90M (Ethanol); 451IFR0GXB (Scopolamine Hydrobromide); EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta); EC 2.7.11.1 (Gsk3b protein, mouse); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170418 [Lr] Data última revisão: 170418 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160609 [St] Status: MEDLINE

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[PMID]: 26945981 [Au] Autor: Dungca NT [Ad] Endereço: The Graduate School, University of Santo Tomas, España Blvd., Manila 1015, Philippines. Electronic address: ninadungca@gmail.com. [Ti] Título: Protective effect of the methanolic leaf extract of Eclipta alba (L.) Hassk. (Asteraceae) against gentamicin-induced nephrotoxicity in Sprague Dawley rats. [So] Source: J Ethnopharmacol;184:18-21, 2016 May 26. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Eclipta alba, also known as Eclipta prostrata, is a weed of the family Asteraceae found in tropical and subtropical regions widely used in herbal medicine, including treatment of renal diseases. AIM OF THE STUDY: This study aims to evaluate the protective effect of the methanolic leaf extract of Eclipta alba on gentamicin-induced nephrotoxicity in rats. MATERIALS AND METHODS: Nephrotoxicity was induced in rats by subcutaneous injection of gentamicin (80mg/kg/day for seven days). Quercetin was used as a positive control. The nephroprotective activity was evaluated by determining blood urea nitrogen, serum creatinine, urinary microprotein, renal catalase and malondialdehyde levels. RESULTS: The extract protected the rat kidneys against gentamicin-induced renal tubular alterations and rises in blood urea nitrogen, serum creatinine, and microprotein levels. Lipid peroxidation and decrement in catalase levels were also ameliorated. CONCLUSION: The study revealed the protective effect of the methanolic leaf extract of E. alba and suggests that the probable mechanism for the nephroprotection by the extract may be due to its good radical scavenging activity and Fe(3+) ion-reducing ability. [Mh] Termos MeSH primário: Eclipta Nefropatias/tratamento farmacológico Extratos Vegetais/uso terapêutico [Mh] Termos MeSH secundário: Animais Antibacterianos Nitrogênio da Ureia Sanguínea Catalase/metabolismo Creatinina/sangue Feminino Gentamicinas Rim/efeitos dos fármacos Rim/metabolismo Rim/patologia Nefropatias/sangue Nefropatias/induzido quimicamente Nefropatias/patologia Peroxidação de Lipídeos/efeitos dos fármacos Metanol/química Fitoterapia Extratos Vegetais/farmacologia Folhas de Planta/química Ratos Sprague-Dawley Solventes/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Gentamicins); 0 (Plant Extracts); 0 (Solvents); AYI8EX34EU (Creatinine); EC 1.11.1.6 (Catalase); Y4S76JWI15 (Methanol) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170106 [Lr] Data última revisão: 170106 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160307 [St] Status: MEDLINE

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