Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.100.355 [Categoria DeCS]
Referências encontradas : 28 [refinar]
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  1 / 28 MEDLINE  
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[PMID]:28623927
[Au] Autor:Jiang Y; Lin Y; Hu YJ; Song XJ; Pan HH; Zhang HJ
[Ad] Endereço:Dispensary of Traditional Chinese Medicine, Hangzhou First People's Hospital, 261 Huansha Road, Hangzhou, 310006, China.
[Ti] Título:Caffeoylquinic acid derivatives rich extract from Gnaphalium pensylvanicum willd. Ameliorates hyperuricemia and acute gouty arthritis in animal model.
[So] Source:BMC Complement Altern Med;17(1):320, 2017 Jun 17.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The Gnaphalium pensylvanicum willd. is used in China as a folk medicine to treat anti-inflammatory, cough and rheumatism arthritis. The aim of this study was to evaluate the potential of the extract of G. pensylvanicum to treat hyperuricemia and acute gouty arthritis in animal model. METHODS: G. pensylvanicum extract was evaluated in an experimental model with potassium oxonate (PO) induced hyperuricemia in mice which was used to evaluate anti-hyperuricemia activity and xanthine oxidase (XO) inhibition. Therapies for acute gouty arthritis was also investigated on monosodium urate (MSU) crystal induced paw edema model. RESULTS: G. pensylvanicum extract showed activity in reducing serum uric acid (Sur) through effect renal glucose transporter 9 (GLUT9), organic anion transporter 1 (OAT1) and urate transporter 1 (URAT1) mainly and inhibited XO activity in vivo of mice with PO induced hyperuricemia. The extract of G. pensylvanicum also showed significant anti-inflammatory activity and reduced the paw swelling on MSU crystal-induced paw edema model. Meanwhile, 13 caffeoylquinic acid derivatives and 1 flavone were identified by UPLC-ESI-MS/MS as the main active component of G. pensylvanicum. CONCLUSIONS: The extract of G. pensylvanicum showed significant effect on evaluated models and therefore may be active agents for the treatment of hyperuricemia and acute gouty arthritis.
[Mh] Termos MeSH primário: Anti-Inflamatórios/administração & dosagem
Artrite Gotosa/tratamento farmacológico
Gnaphalium/química
Supressores da Gota/administração & dosagem
Hiperuricemia/tratamento farmacológico
Extratos Vegetais/administração & dosagem
Ácido Quínico/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Artrite Gotosa/imunologia
Modelos Animais de Doenças
Proteínas Facilitadoras de Transporte de Glucose/genética
Proteínas Facilitadoras de Transporte de Glucose/metabolismo
Supressores da Gota/química
Seres Humanos
Hiperuricemia/genética
Hiperuricemia/imunologia
Rim/efeitos dos fármacos
Rim/metabolismo
Masculino
Camundongos
Fitoterapia
Extratos Vegetais/química
Ácido Quínico/administração & dosagem
Ácido Quínico/química
Ácido Úrico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Glucose Transport Proteins, Facilitative); 0 (Gout Suppressants); 0 (Plant Extracts); 0 (caffeoylquinic acid); 058C04BGYI (Quinic Acid); 268B43MJ25 (Uric Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170619
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1834-9


  2 / 28 MEDLINE  
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[PMID]:27649136
[Au] Autor:Zhang HJ; Hu YJ; Xu P; Liang WQ; Zhou J; Liu PG; Cheng L; Pu JB
[Ad] Endereço:Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Zhejiang Academy of Traditional Chinese Medicine, 132 Tianmushan Road, Hangzhou 310007, China. jian871211@sina.com.
[Ti] Título:Screening of Potential Xanthine Oxidase Inhibitors in Gnaphalium hypoleucum DC. by Immobilized Metal Affinity Chromatography and Ultrafiltration-Ultra Performance Liquid Chromatography-Mass Spectrometry.
[So] Source:Molecules;21(9), 2016 Sep 17.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In this study, a new method based on immobilized metal affinity chromatography (IMAC) combined with ultrafiltration-ultra performance liquid chromatography-mass spectrometry (UF-UPLC-MS) was developed for discovering ligands for xanthine oxidase (XO) in Gnaphalium hypoleucum DC., a folk medicine used in China for the treatment of gout. By IMAC, the high flavonoid content of G. hypoleucum could be determined rapidly and efficiently. UF-UPLC-MS was used to select the bound xanthine oxidase ligands in the mixture and identify them. Finally, two flavonoids, luteolin-4'-O-glucoside and luteolin, were successfully screened and identified as the candidate XO inhibitors of G. hypoleucum. They were evaluated in vitro for XO inhibitory activity and their interaction mechanism was studied coupled with molecular simulations. The results were in favor of the hypothesis that the flavonoids of G. hypoleucum might be the active content for gout treatment by inhibiting XO.
[Mh] Termos MeSH primário: Inibidores Enzimáticos/química
Inibidores Enzimáticos/isolamento & purificação
Flavonoides/química
Flavonoides/isolamento & purificação
Gnaphalium/química
Xantina Oxidase/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Bovinos
Cromatografia de Afinidade
Cromatografia Líquida de Alta Pressão
Espectrometria de Massas
Ultrafiltração
Xantina Oxidase/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Flavonoids); EC 1.17.3.2 (Xanthine Oxidase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE


  3 / 28 MEDLINE  
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[PMID]:27324043
[Au] Autor:Seong YA; Hwang D; Kim GD
[Ad] Endereço:Department of Microbiology, College of Natural Sciences, Pukyong National University, 45, Yongso-ro, Nam-Gu, Busan, 608-737, Korea.
[Ti] Título:The Anti-inflammatory Effect of Gnaphalium affine Through Inhibition of NF-κB and MAPK in Lipopolysaccharide-Stimulated RAW264.7 Cells and Analysis of Its Phytochemical Components.
[So] Source:Cell Biochem Biophys;74(3):407-17, 2016 Sep.
[Is] ISSN:1559-0283
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gnaphalium affine is an annual herbaceous plant that is used as a traditional medicine in some Latin American and Asian countries. However, systematic studies on its anti-inflammatory activity and signaling pathways have not yet been reported. In this study, we investigated the anti-inflammatory effect of G. affine methanol extract in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells and fractioned the methanol extract into hexane, chloroform, ethyl acetate (EtOAc), butyl alcohol (BuOH), and distilled water (DW) by measuring the generation of nitric oxide (NO). G. affine inhibited the generation of NO and prostaglandin E2. The chloroform-soluble fraction most effectively inhibited LPS-stimulated NO production. We also examined the cytotoxicity of G. affine in three normal cell lines: RAW264.7, HEK293, and HaCaT. Cell viability assays showed that the methanol extract and chloroform-soluble fraction of G. affine had no cytotoxic effect on normal cell lines. The expression of pro-inflammatory mediators was also investigated. Western blotting and immunofluorescence showed that G. affine reduces the expression of iNOS, COX-2, and MAPKs, as well as activation of NF-κB in LPS-stimulated RAW264.7 cells. RT-PCR showed that G. affine also negatively regulates inflammatory cytokines at the gene expression level. Taken together, G. affine exerts its anti-inflammatory activity through inhibition of NO generation as a result of inhibiting NF-κB and MAPKs-related inflammatory signaling pathways. In addition, the result of GC-MS analysis revealed the presence of nineteen different types of constituents including guaiacol in the chloroform-soluble fraction of G. affine.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Gnaphalium/química
Proteínas Quinases Ativadas por Mitógeno/metabolismo
NF-kappa B/metabolismo
Compostos Fitoquímicos/farmacologia
Transdução de Sinais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Western Blotting
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Ciclo-Oxigenase 2/genética
Ciclo-Oxigenase 2/metabolismo
Dinoprostona/análise
Ensaio de Imunoadsorção Enzimática
Cromatografia Gasosa-Espectrometria de Massas
Gnaphalium/metabolismo
Células HEK293
Seres Humanos
Lipopolissacarídeos/toxicidade
Macrófagos/citologia
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
Camundongos
Microscopia de Fluorescência
Proteínas Quinases Ativadas por Mitógeno/antagonistas & inibidores
NF-kappa B/antagonistas & inibidores
Óxido Nítrico/metabolismo
Óxido Nítrico Sintase Tipo II/genética
Óxido Nítrico Sintase Tipo II/metabolismo
Fosforilação/efeitos dos fármacos
Compostos Fitoquímicos/química
Células RAW 264.7
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (Phytochemicals); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.99.1 (Cyclooxygenase 2); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160622
[St] Status:MEDLINE
[do] DOI:10.1007/s12013-016-0744-7


  4 / 28 MEDLINE  
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[PMID]:26561928
[Au] Autor:Huang D; Chen Y; Chen W; Liu Y; Yao F; Xue D; Sun L
[Ad] Endereço:Department of Identification of Traditional Chinese Medicine, School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
[Ti] Título:Anti-inflammatory effects of the extract of Gnaphalium affine D. Don in vivo and in vitro.
[So] Source:J Ethnopharmacol;176:356-64, 2015 Dec 24.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Gnaphalium affine D. Don (GA) has been traditionally used as a medicinal herb in China for the treatment of many ailments including rheumatoid arthritis. However, the anti-arthritic mechanism of GA has still not been demonstrated. This study aims to reveal the anti-inflammatory activity and anti-arthritic mechanism of ethanol extract of G. affine D. Don. MATERIALS AND METHODS: Anti-inflammatory potential of GA was analyzed in vivo in carrageenan induced mice paw edema (acute study). Also, in vivo study was applied in collagen-induced arthritis (CIA) rats. In vitro experiments for analyzing the anti-inflammatory potential of GA were performed on rat alveolar macrophages cell line (NR8383). Analysis of nitric oxide release in NR8383 cells was done by Griess reaction. RT-PCR and western blotting experiment was performed to analyze the expression of phosphorylated p65 and IκBα/ß-actin in NF-κB pathway. The production of TNF-α, IL-1ß, and COX-2 in NR8383 cells were measured by enzyme-linked immunosorbent assay. The chemical profile of GA was analyzed by HPLC-VWD. RESULTS: GA significantly reduced the paw volume in carrageenan induced rat paw edema rat at different doses (300 and 600 mg/kg), compared with the standard indomethacin treatment. In CIA, GA can obviously ameliorate the inflammatory symptom, including cytokine, histological symptom and paw swelling. In the vitro study, GA was able to reduce the nitric oxide (NO) levels in NR8383 cells that had been stimulated with lipopolysaccharide (LPS). The level of TNF-α, IL-1ß, and COX-2 was also decreased with GA treatment in NR8383 cells that had been stimulated with lipopolysaccharide (LPS). Interestingly, GA was found to decrease the level of phosphorylated p65 and IκBα in NR8383 cells. Fifteen compounds were identified by HPLC-VWD with the reference substances and verified by LC-MS. CONCLUSIONS: The results of the experiment scientifically validated its traditional use in inflammatory conditions.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Anti-Inflamatórios/uso terapêutico
Gnaphalium
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Artrite Experimental/sangue
Artrite Experimental/tratamento farmacológico
Artrite Experimental/patologia
Carragenina
Linhagem Celular
Edema/tratamento farmacológico
Edema/patologia
Feminino
/patologia
Interleucina-1beta/sangue
Interleucina-1beta/metabolismo
Articulação do Joelho/efeitos dos fármacos
Articulação do Joelho/patologia
Masculino
Camundongos Endogâmicos ICR
NF-kappa B/metabolismo
Óxido Nítrico/metabolismo
Fitoterapia
Ratos Wistar
Fator de Necrose Tumoral alfa/sangue
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Interleukin-1beta); 0 (NF-kappa B); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha); 31C4KY9ESH (Nitric Oxide); 9000-07-1 (Carrageenan)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151113
[St] Status:MEDLINE


  5 / 28 MEDLINE  
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[PMID]:25429747
[Au] Autor:Lin WQ; Xie JX; Wu XM; Yang L; Wang HD
[Ad] Endereço:Nanchang University School of Medicine Graduate School, Nanchang 330006, China; Department of Integrated Traditional Chinese Medicine and Western Medicine, Jiangxi Provincial People's Hospital, Nanchang 330006, China.
[Ti] Título:Inhibition of xanthine oxidase activity by gnaphalium affine extract.
[So] Source:Chin Med Sci J;29(4):225-30, 2014 Dec.
[Is] ISSN:1001-9294
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate the inhibitory effect of Gnaphalium affine extracts on xanthine oxidase (XO) activity in vitro and to analyze the mechanism of this effect. METHODS: In this in vitro study, Kinetic measurements were performed in 4 different inhibitor concentrations and 5 different xanthine concentrations (60, 100, 200, 300, 400 Μmol/L). Dixon and Lineweaver-Burk plot analysis were used to determine Ki values and the inhibition mode for the compounds isolated from Gnaphalium affine extract. RESULTS: Four potent xanthine oxidase inhibitors were found in 95% ethanolic (v/v) Gnaphalium affine extract. Among them, the flavone Eupatilin exhibited the strongest inhibitory effect on XO with a inhibition constant (Ki) of 0.37 Μmol/L, lower than the Ki of allopurinol (4.56 mol/L), a known synthetic XO inhibitor. Apigenin (Ki of 0.56 Μmol/L, a proportion of 0.0053‰ in Gnaphalium affine), luteolin (Ki of 2.63 Μmol/L, 0.0032‰ in Gnaphalium affine) and 5-hydroxy-6,7,3',4'-tetramethoxyflavone (Ki of 3.15 Μmol/L, 0.0043‰ in Gnaphalium affine) also contributed to the inhibitory effect of Gnaphalium affine extract on XO activity. CONCLUSIONS: These results suggest that the use of Gnaphalium affine in the treatment of gout could be attributed to its inhibitory effect on XO. This study provides a rational basis for the traditional use of Gnaphalium affine against gout.
[Mh] Termos MeSH primário: Flavonoides/farmacologia
Gnaphalium/química
Xantina Oxidase/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (gnaphalin); EC 1.17.3.2 (Xanthine Oxidase)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141129
[St] Status:MEDLINE


  6 / 28 MEDLINE  
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[PMID]:24519638
[Au] Autor:Lin L; Shi J; Liu Q; Liao M; Mei L
[Ad] Endereço:College of Horticulture, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China.
[Ti] Título:Cadmium accumulation characteristics of the winter farmland weeds Cardamine hirsuta Linn. and Gnaphalium affine D. Don.
[So] Source:Environ Monit Assess;186(7):4051-6, 2014 Jul.
[Is] ISSN:1573-2959
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In a preliminary study, we found that the cadmium (Cd) concentrations in shoots of the winter farmland weeds Cardamine hirsuta Linn. and Gnaphalium affine D. Don exceeded the critical value of a Cd-hyperaccumulator (100 mg kg(-1)), indicating that these two farmland weeds might be Cd-hyperaccumulators. In this study, we grew these species in soil containing various concentrations of Cd to further evaluate their Cd accumulation characteristics. The biomasses of C. hirsuta and G. affine decreased with increasing Cd concentrations in the soil, while the root/shoot ratio and the Cd concentrations in shoot tissues increased. The Cd concentrations in shoots of C. hirsuta and G. affine reached 121.96 and 143.91 mg kg(-1), respectively, at the soil Cd concentration of 50 mg kg(-1). Both of these concentrations exceeded the critical value of a Cd-hyperaccumulator (100 mg kg(-1)). The shoot bioconcentration factors of C. hirsuta and G. affine were greater than 1. The translocation factor of C. hirsuta was less than 1 and that of G. affine was greater than 1. These findings indicated that C. hirsuta is a Cd-accumulator and G. affine is Cd-hyperaccumulator. Both plants are distributed widely in the field, and they could be used to remediate Cd-contaminated farmland soil in winter.
[Mh] Termos MeSH primário: Cádmio/metabolismo
Cardamine/metabolismo
Gnaphalium/metabolismo
Plantas Daninhas/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Agricultura
Biodegradação Ambiental
Biomassa
Cádmio/análise
Cardamine/química
Monitoramento Ambiental
Gnaphalium/química
Raízes de Plantas
Plantas Daninhas/química
Solo/química
Poluentes do Solo/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 0 (Soil Pollutants); 00BH33GNGH (Cadmium)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140213
[St] Status:MEDLINE
[do] DOI:10.1007/s10661-014-3679-8


  7 / 28 MEDLINE  
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[PMID]:23860277
[Au] Autor:Zheng X; Wang W; Piao H; Xu W; Shi H; Zhao C
[Ad] Endereço:College of Pharmacy, Yanbian University, Yanji 133002, Jilin, China. zhengxing@ybu.edu.cn
[Ti] Título:The genus Gnaphalium L. (Compositae): phytochemical and pharmacological characteristics.
[So] Source:Molecules;18(7):8298-318, 2013 Jul 15.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The genus Gnaphalium, a herb distributed worldwide, comprises approximately 200 species of the Compositae (Asteraceae) family that belongs to the tribe Gnaphalieae. Some species are traditionally used as wild vegetables and in folk medicine. This review focuses on the phytochemical investigations and biological studies of plants from the genus Gnaphalium over the past few decades. More than 125 chemical constituents have been isolated from the genus Gnaphalium, including flavonoids, sesquiterpenes, diterpenes, triterpenes, phytosterols, anthraquinones, caffeoylquinic acid derivatives, and other compounds. The extracts of this genus, as well as compounds isolated from it, have been demonstrated to possess multiple pharmacological activities such as antioxidant, antibacterial and antifungal, anti-complement, antitussive and expectorant, insect antifeedant, cytotoxic, anti-inflammatory, antidiabetic and antihypouricemic properties. The present review compiles the information available on this genus because of its relevance to food and ethnopharmacology and the potential therapeutic uses of these species.
[Mh] Termos MeSH primário: Gnaphalium/química
Compostos Fitoquímicos/farmacologia
[Mh] Termos MeSH secundário: Etnofarmacologia
Gnaphalium/classificação
Medicina Herbária
Medicina Tradicional
Compostos Fitoquímicos/química
Compostos Fitoquímicos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Phytochemicals)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:130717
[Lr] Data última revisão:
130717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130718
[St] Status:MEDLINE
[do] DOI:10.3390/molecules18078298


  8 / 28 MEDLINE  
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[PMID]:23823875
[Au] Autor:Li J; Huang D; Chen W; Xi Z; Chen C; Huang G; Sun L
[Ad] Endereço:School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
[Ti] Título:Two new phenolic glycosides from Gnaphalium affine D. Don and their anti-complementary activity.
[So] Source:Molecules;18(7):7751-60, 2013 Jul 03.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Two new phenolic glycosides, named gnaphaffine A and B (compounds 1 and 2), were isolated from Gnaphalium affine. together with six known compounds, including caffeic acid (3), everlastoside L (4), isorhamnetin-7-O-ß-D-glucopyranoside (5), quercetin- 3-O-ß-D-glucopyranoside (6), scutellarein-7-O-ß-D-glucoside (7) and api-genin-7-O-ß-D- glucopyranoside (8). Their structures were elucidated by spectroscopic methods, including ESI-MS and 2D NMR spectroscopy (HMQC and HMBC). All compounds were evaluated for their anti-complementary activity on the classical pathway of the complement system in vitro.
[Mh] Termos MeSH primário: Inativadores do Complemento/farmacologia
Proteínas do Sistema Complemento/efeitos dos fármacos
Glicosídeos/farmacologia
Gnaphalium/metabolismo
Fenóis/farmacologia
[Mh] Termos MeSH secundário: Inativadores do Complemento/química
Glicosídeos/análise
Glicosídeos/isolamento & purificação
Medicina Tradicional Chinesa
Fenóis/análise
Fenóis/química
Preparações de Plantas/análise
Preparações de Plantas/química
Preparações de Plantas/farmacologia
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Complement Inactivating Agents); 0 (Glycosides); 0 (Phenols); 0 (Plant Preparations); 9007-36-7 (Complement System Proteins)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:130704
[Lr] Data última revisão:
130704
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130705
[St] Status:MEDLINE
[do] DOI:10.3390/molecules18077751


  9 / 28 MEDLINE  
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[PMID]:23685244
[Au] Autor:Zeng WC; Zhang WC; Zhang WH; He Q; Shi B
[Ad] Endereço:The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu, Sichuan 610065, PR China.
[Ti] Título:The antioxidant activity and active component of Gnaphalium affine extract.
[So] Source:Food Chem Toxicol;58:311-7, 2013 Aug.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The antioxidant activity of Gnaphalium affine extract (GAE) against H2O2-induced oxidative injury in Caco-2 cells was evaluated, and the main antioxidant component was isolated and identified by column chromatography, high performance liquid chromatography, time-of-flight mass spectrometer and nuclear magnetic resonance. In vitro assays, GAE showed remarkable antioxidant activity to scavenge free radicals (ABTS, DPPH, superoxide and hydroxyl radicals), inhibit lipid peroxidation and show reducing power. In food system, GAE exhibited the obvious capacity to inhibit the oxidation of peanut oil and lard, which may be attributed to its high content of phenolic compounds. Moreover, GAE could effectively protect Caco-2 cell against H2O2-induced oxidative injury. With the isolation and purification by chromatography, quercetin was identified as the main antioxidant component of GAE, which was capable of scavenging ABTS, DPPH, superoxide and hydroxyl radicals. These results suggest that G. affine is a potential source for preparing functional foods and nutraceuticals in food industry.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Gnaphalium/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Células CACO-2
Seres Humanos
Peroxidação de Lipídeos/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:130705
[Lr] Data última revisão:
130705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130521
[St] Status:MEDLINE


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[PMID]:23019902
[Au] Autor:Sun Q; Lu Y; Wu SQ; Yao S; Zhang J
[Ad] Endereço:College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
[Ti] Título:[Study on the chemical constituents from Gnaphalium hypoleucum].
[So] Source:Zhong Yao Cai;35(4):566-8, 2012 Apr.
[Is] ISSN:1001-4454
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To research the chemical constituents from Gnaphalium hypoleucum. METHODS: Compounds were separated by using silica gel, ODS, Sephadex LH-20 chromatography and their structures were elucidated by means of spectral data analysis. RESULTS: Ten compounds were isolated and identified as tetracosanoic acid (1), 5-hydroxy-3,6,7,8-tetramethoxyflavone (2), beta-sitosterol (3), 5-hydroxy-3,6,7,8,4'-pentamethoxyflavone (4), 5-hydroxy-3,6, 7,8,3',4'-hexamethoxyflavone (5), apigenin (6), luteolin (7), quercetin (8), luteolin4'-O-beta-D-glucoside (9), quercetin-4'-O-beta-D-glucoside (10). CONCLUSION: All of these compounds are isolated from this plant for the first time. Compound 5 is isolated from the genus for the first time.
[Mh] Termos MeSH primário: Flavonoides/análise
Gnaphalium/química
Extratos Vegetais/análise
[Mh] Termos MeSH secundário: Flavonoides/química
Flavonoides/isolamento & purificação
Componentes Aéreos da Planta/química
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Espectrofotometria Ultravioleta
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (Plant Extracts)
[Em] Mês de entrada:1302
[Cu] Atualização por classe:121001
[Lr] Data última revisão:
121001
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121002
[St] Status:MEDLINE



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