Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.100.596 [Categoria DeCS] Referências encontradas : 95 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28494350 [Au] Autor: Lee HS; Kim SW; Abd El-Aty AM; Chung HS; Kabir MH; Rahman MM; Jeong JH; Chang BJ; Shin HC; Shim JH [Ad] Endereço: Natural Products Chemistry Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Yongbong-ro 77, Buk-gu, Gwangju 500-757, Republic of Korea. [Ti] Título: Liquid chromatography-tandem mass spectrometry quantification of acetamiprid and thiacloprid residues in butterbur grown under regulated conditions. [So] Source: J Chromatogr B Analyt Technol Biomed Life Sci;1055-1056:172-177, 2017 Jun 15. [Is] ISSN: 1873-376X [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: An analytical method was developed to quantify the residual levels of the neonicotinoid insecticides, acetamiprid and thiacloprid, in field-incurred butterbur samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples were extracted with acetonitrile and partitioned with dichloromethane. After partitioning, purification was conducted using a Florisil cartridge. Linearity of a matrix-matched calibration curve of the two compounds over a concentration range of 0.004-0.4µg/g was excellent, with determination coefficients (R )≥0.9998. The limits of detection (LOD) and quantitation (LOQ) for both acetamiprid and thiacloprid were 0.0006 and 0.002mg/kg, respectively. The average recoveries for acetamiprid and thiacloprid at two spiking levels (0.02 and 0.1mg/kg, i.e., 10×LOQ and 50×LOQ) were between 78.23 to 82.17%, with relative standard deviations (RSDs)≤7.22%. The method was successfully applied to field-incurred samples treated with a commercial pesticide product, either once (zero or 7 days before harvest) or twice (0 and 7, 7 and 14, or 14 and 21 days before harvest). The highest and lowest residues were obtained for the 7 and 0 days' treatment and the 21 and 14 days' treatment, respectively. The developed method is simple and accurate and can be extrapolated to other leafy vegetables. [Mh] Termos MeSH primário: Inseticidas/análise Petasites/química Piridinas/análise Espectrometria de Massas em Tandem/métodos Tiazinas/análise [Mh] Termos MeSH secundário: Cromatografia Líquida/métodos Limite de Detecção Neonicotinoides Petasites/crescimento & desenvolvimento [Pt] Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Insecticides); 0 (Neonicotinoids); 0 (Pyridines); 0 (Thiazines); 5HL5N372P0 (acetamiprid); DSV3A944A4 (thiacloprid) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170512 [St] Status: MEDLINE

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[PMID]: 27979259 [Au] Autor: Rahman MM; Abd El-Aty AM; Kim SW; Na TW; Shin HC; Hong SM; Shim JH [Ad] Endereço: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea. [Ti] Título: A simple extraction method for the detection and quantification of polyoxin D, a nucleoside antibiotic, in butterbur using UPLC-MS/MS. [So] Source: Food Chem;221:683-688, 2017 Apr 15. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: An effective analytical method was developed for the detection and quantification of polyoxin D in butterbur using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were extracted using acidified deionised water and purified via solid-phase extraction (SPE) using an HLB cartridge. An external matrix-matched standard calibration curve was prepared, which provided an excellent linearity with a coefficient of determination (R ) ⩾0.999. Limits of detection (LOD) and quantification (LOQ) were 0.015 and 0.0.05µg/g, respectively. The developed method was validated in terms of recovery performance using two fortification levels in triplicate. The storage stabilities of the various field samples were also determined. Our method provided consistent recovery (86.26-87.37%) with a relative standard deviation (RSD) of <5%, and was successfully applied to field-treated butterbur grown under greenhouse conditions and collected at various times following commercial fungicide application. As expected, a gradual degradation of polyoxin D was observed, with a half-life (t ) of 2.11d being recorded. Finally, we propose that the developed method can be extrapolated to other crops for routine analysis and can be used to determine the pre-harvest intervals (PHIs), thus preventing the development of antibiotic resistance genes in humans and in the environment. [Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos Petasites/química Espectrometria de Massas em Tandem/métodos [Mh] Termos MeSH secundário: Nucleosídeos de Pirimidina/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Pyrimidine Nucleosides); A2468O1N7D (polyoxorim) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 171003 [Lr] Data última revisão: 171003 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161217 [St] Status: MEDLINE

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[PMID]: 27451267 [Au] Autor: Kataoka Y; Tamagawa-Mineoka R; Masuda K; Katoh N [Ad] Endereço: Department of Dermatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan. [Ti] Título: Anaphylaxis to Japanese butterbur scapes. [So] Source: Allergol Int;66(1):141-142, 2017 Jan. [Is] ISSN: 1440-1592 [Cp] País de publicação: England [La] Idioma: eng [Mh] Termos MeSH primário: Anafilaxia Hipersensibilidade Alimentar Petasites/efeitos adversos [Mh] Termos MeSH secundário: Feminino Seres Humanos Meia-Idade [Pt] Tipo de publicação: CASE REPORTS; LETTER [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170123 [Lr] Data última revisão: 170123 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160725 [St] Status: MEDLINE

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[PMID]: 26674157 [Au] Autor: Choi YW; Lee KP; Kim JM; Kang S; Park SJ; Lee JM; Moon HR; Jung JH; Lee YG; Im DS [Ad] Endereço: Department of Horticultural Bioscience, College of Natural Resources & Life Science, Pusan National University, Miryang-si, Gyeongsangnam 627-706, Republic of Korea. [Ti] Título: Petatewalide B, a novel compound from Petasites japonicus with anti-allergic activity. [So] Source: J Ethnopharmacol;178:17-24, 2016 Feb 03. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: The giant butterbur Petasites japonicus is used to treat asthma and allergic diseases in traditional Korean, Japanese, and Chinese medicine. AIM OF THE STUDY: To elucidate the anti-allergic effect of Petasites genus, we studied effects of several compounds from Petasites japonicus leaves and found a novel bakkenolide-type sesquiterpine. In the present study, anti-allergic and anti-inflammatory effects of the new compound was examined using in vivo and in vitro experiments. MATERIALS AND METHODS: The novel compound was isolated from Petasites japonicus leaves and named petatewalide B. Antigen-induced degranulation and Ca(2+) mobilization were measured in RBL-2H3 mast cells by measuring ß-hexosaminidase activity and fluorescence change of Ca(2+) probe, fura-2. Induction of inducible nitric oxide synthase and cyclooxygenase 2 was measured by Western blotting in peritoneal macrophages. In addition, ovalbumin-induced asthma model was used for in vivo efficacy test of petatewalide B. Membrane potential was estimated by measuring fluorescence change of DiBAC in C6 glioma cells. RESULTS: Petatewalide B inhibited the antigen-induced degranulation of ß-hexosaminidase in RBL-2H3 mast cells, but did not affect antigen-induced Ca(2+) increase in the cells. Petatewalide B also showed inhibition of the LPS-induced induction of iNOS, but not COX-2 in mouse peritoneal macrophages. Nitric oxide production was also inhibited by petatewalide B in macrophages. In the ovalbumin-induced asthma model, petatewalide B strongly inhibited accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid. Petatewalide B increased the membrane potential of C6 glioma cells in a concentration-dependent manner. CONCLUSION: Petatewalide B from Petasites genus not only has anti-allergic and anti-inflammatory effects but also induces a transient increase of membrane potential in C6 glioma cells. [Mh] Termos MeSH primário: Antialérgicos/química Antialérgicos/farmacologia Asma/tratamento farmacológico Petasites/química Extratos Vegetais/química Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Animais Anti-Inflamatórios/química Anti-Inflamatórios/farmacologia Cálcio/metabolismo Linhagem Celular Tumoral Ciclo-Oxigenase 2/metabolismo Masculino Mastócitos/efeitos dos fármacos Mastócitos/metabolismo Camundongos Camundongos Endogâmicos BALB C Óxido Nítrico/metabolismo Óxido Nítrico Sintase Tipo II/metabolismo Folhas de Planta/química Ratos Sesquiterpenos/química Sesquiterpenos/farmacologia beta-N-Acetil-Hexosaminidases/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Allergic Agents); 0 (Anti-Inflammatory Agents); 0 (Plant Extracts); 0 (Sesquiterpenes); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.99.1 (Cyclooxygenase 2); EC 3.2.1.52 (beta-N-Acetylhexosaminidases); SY7Q814VUP (Calcium) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151218 [St] Status: MEDLINE

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[PMID]: 26207817 [Au] Autor: Kang HR; Lee YA; Kim YH; Lee DG; Kim BJ; Kim KJ; Kim BG; Oh MG; Han CK; Lee S; Ryu BY [Ad] Endereço: Department of Animal Science and Technology, Chung-Ang University, Anseong, Republic of Korea. [Ti] Título: Petasites japonicus Stimulates the Proliferation of Mouse Spermatogonial Stem Cells. [So] Source: PLoS One;10(7):e0133077, 2015. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Oriental natural plants have been used as medical herbs for the treatment of various diseases for over 2,000 years. In this study, we evaluated the effect of several natural plants on the preservation of male fertility by assessing the ability of plant extracts to stimulate spermatogonial stem cell (SSC) proliferation by using a serum-free culture method. In vitro assays showed that Petasites japonicus extracts, especially the butanol fraction, have a significant effect on germ cells proliferation including SSCs. The activity of SSCs cultured in the presence of the Petasites japonicus butanol fraction was confirmed by normal colony formation and spermatogenesis following germ cell transplantation of the treated SSCs. Our findings could lead to the discovery of novel factors that activate SSCs and could be useful for the development of technologies for the prevention of male infertility. [Mh] Termos MeSH primário: Petasites/química Extratos Vegetais/farmacologia Espermatogônias/efeitos dos fármacos Espermatogônias/metabolismo Células-Tronco/efeitos dos fármacos Células-Tronco/metabolismo [Mh] Termos MeSH secundário: Animais Técnicas de Cultura de Células Proliferação Celular/efeitos dos fármacos Células Cultivadas Meios de Cultura Livres de Soro Fertilidade/efeitos dos fármacos Masculino Camundongos [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Culture Media, Serum-Free); 0 (Plant Extracts) [Em] Mês de entrada: 1605 [Cu] Atualização por classe: 150731 [Lr] Data última revisão: 150731 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150725 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0133077

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[PMID]: 26094206 [Au] Autor: Schenk A; Siewert B; Toff S; Drewe J [Ad] Endereço: Departments of Analytical Development, Max Zeller Söhne AG, Seeblickstrasse 4, CH 8590 Romanshorn, Switzerland. Electronic address: alexander.schenk@zellerag.ch. [Ti] Título: UPLC TOF MS for sensitive quantification of naturally occurring pyrrolizidine alkaloids in Petasites hybridus extract (Ze 339). [So] Source: J Chromatogr B Analyt Technol Biomed Life Sci;997:23-9, 2015 Aug 01. [Is] ISSN: 1873-376X [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Due to increasing regulatory awareness of their hepatotoxic, genotoxic and possibly carcinogenic potential, pyrrolizidine alkaloid (PA) content has to be thoroughly monitored in herbal medicinal preparations. Recently, new very low PA regulatory threshold concentrations have been requested by the authorities. Therefore, a highly sensitive and reproducible UPLC TOF MS method for the quantification of the PAs senkirkine, senecionine, seneciphylline, senecionine-N-oxide and seneciphylline-N-oxide in a CO2-extract of Petasites hybridus leaves (Ze 339) has been developed. The limit of quantification (LOQ) was 2ppb for all PAs. Recovery at the LOQ was between 88.9 and 141.9%, the repeatability precision between 3.5 and 13.6%. Linearity of the five PAs showed correlation coefficients between 0.9995 and 0.9998 and coefficients of variation between 7.44 and 8.56%. A working range between 2 ppb and 200 ppb could be fixed. In the tested batches of the P. hybridus extract Ze 339, the absence of PAs could be demonstrated. In conclusion, this assay allows to determine trace PA concentrations in P. hybridus extract Ze 339, making it suitable for analytical PA monitoring in accordance with regulatory requirements. [Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos Petasites/química Extratos Vegetais/química Alcaloides de Pirrolizidina/análise Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos [Mh] Termos MeSH secundário: Modelos Lineares Alcaloides de Pirrolizidina/química Reprodutibilidade dos Testes Sensibilidade e Especificidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Extracts); 0 (Pyrrolizidine Alkaloids) [Em] Mês de entrada: 1601 [Cu] Atualização por classe: 150708 [Lr] Data última revisão: 150708 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150622 [St] Status: MEDLINE

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[PMID]: 25924658 [Au] Autor: Soleimani A; Asadi J; Rostami-Charati F; Gharaei R [Ti] Título: High Cytotoxicity and Apoptotic Effects of Natural Bioactive Benzofuran Derivative on the MCF-7 Breast Cancer Cell Line. [So] Source: Comb Chem High Throughput Screen;18(5):505-13, 2015. [Is] ISSN: 1875-5402 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: This study was focused on evaluation of the cytotoxicity and apoptotic affects of benzofuran derivative on MCF-7 breast cancer cell line. This effective compound was isolated from the root of Petasites hybridus plant. For experiments, the MCF-7 cells were treated with several concentrations (0-500µM) of 1-(6-hydroxy-2- isopropenyl-1-benzofuran-5-yl)-1-ethanone 1 at different times. In this study, test of neutral red was also employed for cytotoxicity assay and quantity of P53, P21. Bax genes expression was analyzed using Real-Time PCR and ELISA techniques. Results show that compound 1 has cytotoxicity and apoptotic effects on Human breast cancer (Michigan Cancer Foundation-7) MCF-7 cells. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Apoptose/efeitos dos fármacos Benzofuranos/farmacologia Produtos Biológicos/farmacologia Neoplasias da Mama/patologia [Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química Antineoplásicos Fitogênicos/isolamento & purificação Benzofuranos/química Benzofuranos/isolamento & purificação Produtos Biológicos/química Produtos Biológicos/isolamento & purificação Proliferação Celular/efeitos dos fármacos Relação Dose-Resposta a Droga Ensaios de Seleção de Medicamentos Antitumorais Seres Humanos Células MCF-7 Estrutura Molecular Petasites/química Raízes de Plantas/química Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Benzofurans); 0 (Biological Products); LK6946W774 (benzofuran) [Em] Mês de entrada: 1602 [Cu] Atualização por classe: 150729 [Lr] Data última revisão: 150729 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150501 [St] Status: MEDLINE

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[PMID]: 25663480 [Au] Autor: Hisayoshi T; Shinomura M; Yokokawa K; Kuze I; Konishi A; Kawaji K; Kodama EN; Hata K; Takahashi S; Nirasawa S; Sakuda S; Yasukawa K [Ad] Endereço: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan. [Ti] Título: Inhibition of the DNA polymerase and RNase H activities of HIV-1 reverse transcriptase and HIV-1 replication by Brasenia schreberi (Junsai) and Petasites japonicus (Fuki) components. [So] Source: J Nat Med;69(3):432-40, 2015 Jul. [Is] ISSN: 1861-0293 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) possesses two distinct enzymatic activities: those of RNA- and DNA-dependent DNA polymerases and RNase H. In the current HIV-1 therapy, all HIV-1 RT inhibitors inhibit the activity of DNA polymerase, but not that of RNase H. We previously reported that ethanol and water extracts of Brasenia schreberi (Junsai) inhibited the DNA polymerase activity of HIV-1 RT [Hisayoshi et al. (2014) J Biol Macromol 14:59-65]. In this study, we screened 43 edible plants and found that ethanol and water extracts of Brasenia schreberi and water extract of Petasites japonicus strongly inhibit not only the activity of DNA polymerase to incorporate dTTP into poly(rA)-p(dT)15 but also the activity of RNase H to hydrolyze the RNA strand of an RNA/DNA hybrid. In addition, these three extracts inhibit HIV-1 replication in human cells, with EC50 values of 1-2 µg/ml. These results suggest that Brasenia schreberi and Petasites japonicus contain substances that block HIV-1 replication by inhibiting the DNA polymerase activity and/or RNase H activity of HIV-1 RT. [Mh] Termos MeSH primário: Fármacos Anti-HIV/química Transcriptase Reversa do HIV/antagonistas & inibidores HIV-1/fisiologia Petasites/química Extratos Vegetais/química Inibidores da Transcriptase Reversa/química Ribonuclease H/antagonistas & inibidores [Mh] Termos MeSH secundário: Fármacos Anti-HIV/farmacologia DNA Polimerase Dirigida por DNA/química Avaliação Pré-Clínica de Medicamentos Transcriptase Reversa do HIV/química HIV-1/efeitos dos fármacos HIV-1/enzimologia Células HeLa Seres Humanos Extratos Vegetais/farmacologia Inibidores da Transcriptase Reversa/farmacologia Replicação Viral/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-HIV Agents); 0 (Plant Extracts); 0 (Reverse Transcriptase Inhibitors); EC 2.7.7.- (reverse transcriptase, Human immunodeficiency virus 1); EC 2.7.7.49 (HIV Reverse Transcriptase); EC 2.7.7.7 (DNA-Directed DNA Polymerase); EC 3.1.26.4 (Ribonuclease H) [Em] Mês de entrada: 1601 [Cu] Atualização por classe: 171104 [Lr] Data última revisão: 171104 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150210 [St] Status: MEDLINE [do] DOI: 10.1007/s11418-015-0885-9

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[PMID]: 25588954 [Au] Autor: Tagami K; Uchida S [Ad] Endereço: Office of Biospheric Assessment for Waste Disposal, National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Chiba, 263-8555, Japan. Electronic address: k_tagami@nirs.go.jp. [Ti] Título: Effective half-lives of ¹³7Cs in giant butterbur and field horsetail, and the distribution differences of potassium and ¹³7Cs in aboveground tissue parts. [So] Source: J Environ Radioact;141:138-45, 2015 Mar. [Is] ISSN: 1879-1700 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Concentrations of (137)Cs and (40)K in different tissues of edible wild herbaceous plants, that is, leaf blade and petiole for giant butterbur (Petasites japonicas (Siebold et Zucc.) Maxim.), and leaf, stem and strobilus for fertile shoot of field horsetail (Equisetum arvense L.) were measured in 2012-2014 to clarify the effect in Japan from the Fukushima Daiichi Nuclear Power Plant accident. The concentrations of (137)Cs decreased with time with effective half-lives of ca. 450 d and 360 d for giant butterbur and field horsetail, respectively. The ANOVA test revealed that (40)K and (137)Cs distributions in leaf blade and petiole for giant butterbur and leaf and stem for field horsetail were different. Therefore, other plants, leaf and stem for Japanese knotweed (Fallopia japonica (Houtt.) Ronse Decr.) and Canada goldenrod (Solidago canadensis L.), and leaf blade and petiole for gingko (Ginkgo biloba L.) and Someiyoshino cherry (Cerasus × yedoensis (Matsum.) A.V.Vassil. 'Somei-yoshino') were collected from the same sampling field and their (137)Cs and (40)K concentrations were compared to those in the giant butterbur and field horsetail parts. For (137)Cs, concentrations in leaf blade and leaf parts were 1.1-6.0 times higher than those in petiole and stem parts for all six plants. On the other hand, (40)K concentrations in leaf blade and leaf parts were 0.40-0.97 of those observed in petiole and stem parts. Discrimination ratios of (40)K/(137)Cs of leaf blade to petiole or leaf to stem were then calculated and they ranged from 0.09 to 0.57. These results suggested that Cs and K did not behave similarly in these plants. Thus, to understand the radiocesium fate in plants, K measurement results should not be used as an analog for Cs behavior although Cs is known to have a similar chemical reactivity to that of K. [Mh] Termos MeSH primário: Radioisótopos de Césio/metabolismo Equisetum/metabolismo Petasites/metabolismo Plantas Comestíveis/metabolismo Radioisótopos de Potássio/metabolismo Poluentes Radioativos do Solo/metabolismo [Mh] Termos MeSH secundário: Meia-Vida Japão Folhas de Planta/metabolismo Caules de Planta/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Cesium Radioisotopes); 0 (Potassium Radioisotopes); 0 (Soil Pollutants, Radioactive) [Em] Mês de entrada: 1512 [Cu] Atualização por classe: 150319 [Lr] Data última revisão: 150319 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150116 [St] Status: MEDLINE

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[PMID]: 25316419 [Au] Autor: Kim DW; Choi JH; Park SE; Kim S; Sapkota K; Kim SJ [Ti] Título: Purification and characterization of a fibrinolytic enzyme from Petasites japonicus. [So] Source: Int J Biol Macromol;72:1159-67, 2015 Jan. [Is] ISSN: 1879-0003 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: A new, direct-acting chymotrypsin-like fibrinolytic serine protease was purified from Petasites japonicus, a medicinal herb. The molecular mass of the discovered enzyme was estimated to be 40.0 kDa as determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis, fibrin zymography, and gel filtration chromatography. The N-terminal sequence of the purified enzyme was determined to be GQEDHFLQVSLTSA. The proteolytic activity of the enzyme was found to be inhibited by serine protease inhibitors phenylmethylsulfonyl fluoride and 4-(amidinophenyl) methanesulfonyl fluoride. An assay of enzyme activity on fibrin plates revealed that it could hydrolyze the fibrin directly. The enzyme displayed a potent fibrin(ogen)olytic activity, hydrolyzing the Aα-, α-, and Bß-subunits of the human fibrinogen. The enzyme prolonged activated partial thromboplastin time and had little effect on prothrombin time. It prevented carrageenan-induced thrombus formation in mouse tails and did not increase the bleeding time. Our findings indicate that the extracted enzyme we present here has the potential for clinical use as an agent for the treatment of thrombosis. [Mh] Termos MeSH primário: Fibrinólise Petasites/enzimologia Serina Proteases/isolamento & purificação [Mh] Termos MeSH secundário: Amidas/metabolismo Sequência de Aminoácidos Animais Anticoagulantes/farmacologia Antitrombinas/farmacologia Eletroforese em Gel de Poliacrilamida Fibrinogênio/metabolismo Fibrinólise/efeitos dos fármacos Seres Humanos Masculino Camundongos Endogâmicos ICR Dados de Sequência Molecular Análise de Sequência de Proteína Serina Proteases/química Trombose/patologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Amides); 0 (Anticoagulants); 0 (Antithrombins); 9001-32-5 (Fibrinogen); EC 3.4.- (Serine Proteases) [Em] Mês de entrada: 1508 [Cu] Atualização por classe: 150127 [Lr] Data última revisão: 150127 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 141016 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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