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[PMID]:28372735
[Au] Autor:Su B; Zhou M; Xu H; Zhang X; Li Y; Su H; Xiang B
[Ad] Endereço:State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address: shu
[Ti] Título:Photosynthesis and biochemical responses to elevated O in Plantago major and Sonchus oleraceus growing in a lowland habitat of northern China.
[So] Source:J Environ Sci (China);53:113-121, 2017 Mar.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A field experiment was carried out to compare the responses to ozone (O ) in two common herbaceous plant species, Plantago major L. and Sonchus oleraceus L., by building open-top growth chambers in situ to simulate O stress (+O , 85±5ppb, 9hr/day for 30days) in a lowland habitat in Inner Mongolia, Northern China. Responses to O of gas exchange, chlorophyll a fluorescence, leaf pigment content, antioxidant capability, soluble protein content, membrane lipid peroxidation and dark respiration (R ) were analyzed. Results showed that elevated O exposure significantly reduced the light-saturated net photosynthesis (P ), stomatal conductance (g ) and transpiration rate (E) in both species. Although non-significant interactive effect between species and O on P was analyzed, the reduction in P in S. oleraceus might be due primarily to the higher fraction of close PSII reaction centers and impaired activities of plant mesophyll cells as evidences by decreased maximum efficiency of PSII photochemistry after dark adapted state (F /F ) and unchanged intercellular CO concentration (C ). Besides, biochemical analysis showed that S. oleraceus had lower antioxidant ability compared to P. major. As a result, S. oleraceus was damaged to the larger extent in terms of lipid peroxidation and visible O injury, indicating that S. oleraceus was more sensitive to O than P. major. Our results indicated that wild herbaceous plant species growing in a lowland habitat in sandy grassland were sensitive to O stress and S. oleraceus can be considered as one of the bio-indicators for high O concentration in semi-arid grassland of northern China.
[Mh] Termos MeSH primário: Poluentes Atmosféricos/toxicidade
Ozônio/toxicidade
Fotossíntese/efeitos dos fármacos
Plantago/fisiologia
Sonchus/fisiologia
[Mh] Termos MeSH secundário: China
Ecossistema
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 66H7ZZK23N (Ozone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE


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[PMID]:28112016
[Au] Autor:Li Q; Dong DD; Huang QP; Li J; Du YY; Li B; Li HQ; Huyan T
[Ad] Endereço:a Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences , Northwestern Polytechnical University , Xi'an , Shaanxi , P. R. China.
[Ti] Título:The anti-inflammatory effect of Sonchus oleraceus aqueous extract on lipopolysaccharide stimulated RAW 264.7 cells and mice.
[So] Source:Pharm Biol;55(1):799-809, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Sonchus oleraceus L. (Asteraceae) (SO) is a dietary and traditional medicinal plant in China. However, its underlying mechanism of action as an anti-inflammatory agent is not known. OBJECTIVE: This study evaluates the anti-inflammatory activity of aqueous extract of SO. MATERIALS AND METHODS: The extract of SO was used to treat RAW 264.7 cells (in the working concentrations of 500, 250, 125, 62.5, 31.3 and 15.6 µg/mL) for 24 h. Pro-inflammatory cytokines and mediators produced in LPS-stimulated RAW 264.7 cells were assessed. Meanwhile, the expression level of TLR-4, COX-2, pSTATs and NF-κB was tested. Moreover, the anti-inflammatory activity of the extract in vivo was assessed using xylene-induced mouse ear oedema model and the anti-inflammatory compounds in the extracts were analyzed by HPLC-MS. RESULTS: SO extract significantly inhibited the production of pro-inflammatory cytokines and mediators at gene and protein levels with the concentration of 31.3 µg/mL, and suppressed the expression of TLR-4, COX-2, NF-κB and pSTAT in RAW 264.7 cells. The anti-inflammatory activity of SO in vivo has significant anti-inflammatory effects with the concentration of 250 and 125 mg/kg, and less side effect on the weights of the mice at the concentration of 250 mg/kg. Moreover, HPLC-MS analysis revealed that the anti-inflammatory compounds in the extract were identified as villosol, ferulaic acid, ß-sitosterol, ursolic acid and rutin. DISCUSSION AND CONCLUSION: This study indicated that SO extract has anti-inflammatory effects in vitro and in vivo, which will be further developed as novel pharmacological strategies in order to defeat inflammatory diseases.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Lipopolissacarídeos/farmacologia
Extratos Vegetais/farmacologia
Sonchus
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular/efeitos dos fármacos
Inibidores de Ciclo-Oxigenase 2/farmacologia
Citocinas/antagonistas & inibidores
Citocinas/genética
Masculino
Camundongos
NF-kappa B/análise
Extratos Vegetais/análise
Células RAW 264.7
Sonchus/química
Receptor 4 Toll-Like/antagonistas & inibidores
Receptor 4 Toll-Like/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cyclooxygenase 2 Inhibitors); 0 (Cytokines); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (Plant Extracts); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 4)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170303
[Lr] Data última revisão:
170303
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1080/13880209.2017.1280514


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[PMID]:28042942
[Au] Autor:Masi M; Cimmino A; Boari A; Tuzi A; Zonno MC; Baroncelli R; Vurro M; Evidente A
[Ad] Endereço:Department of Chimical Sciences, University of Naples Federico II, Complesso Universitario Monte San'Angelo , Via Cintia 4, 80126 Napoli, Italy.
[Ti] Título:Colletochlorins E and F, New Phytotoxic Tetrasubstituted Pyran-2-one and Dihydrobenzofuran, Isolated from Colletotrichum higginsianum with Potential Herbicidal Activity.
[So] Source:J Agric Food Chem;65(6):1124-1130, 2017 Feb 15.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new tetrasubstituted pyran-2-one and a new dihydrobenzofuran, named colletochlorins E and F (1 and 2, respectively), were isolated from the culture filtrates of the fungus Colletotrichum higginsianum together with the already known colletochlorin A, 4-chloroorcinol, and colletopyrone. Colletochlorin E, the main metabolite, and colletochlorin F were characterized by spectroscopic (NMR, HRESIMS) and chemical methods as 3-[7-chloro-4-hydroxy-2-(1-hydroxy-1-methylethyl)-6-methyl-2,3-dihydrobenzofuran-5-ylmethyl]-4-hydroxy-5,6-dimethylpyran-2-one and 7-chloro-2-(1-hydroxy-1-methylethyl)-6-methyl-2,3-dihydrobenzofuran-4-ol, respectively. The absolute configuration 2'S of 1 was deduced by X-ray diffractometric analysis, whereas 2S of 2 was deduced by comparison of its NMR and CD data with those of 1. When assayed by leaf puncture on Sonchus arvensis and tomato leaves, 2 caused quite large necrosis (>1 cm), whereas 4-chloroorcinol proved to be the most active compound. These results were confirmed by those obtained in assays on Lemna minor and Phelipanche ramosa seed germination. Furthermore 1, colletochlorin A and colletopyrone were less or modestly active in the latter assay, respectively. Interestingly, the phytotoxicity was not associated with an antibiotic activity, whereas only 4-chloroorcinol and colletochlorin F exhibited zootoxic activity.
[Mh] Termos MeSH primário: Colletotrichum/química
Herbicidas/química
Herbicidas/farmacologia
Compostos Heterocíclicos de 4 ou mais Anéis/química
Compostos Heterocíclicos de 4 ou mais Anéis/farmacologia
[Mh] Termos MeSH secundário: Animais
Artemia/efeitos dos fármacos
Benzofuranos/química
Colletotrichum/metabolismo
Herbicidas/isolamento & purificação
Compostos Heterocíclicos de 4 ou mais Anéis/isolamento & purificação
Lycopersicon esculentum/efeitos dos fármacos
Estrutura Molecular
Pironas/química
Metabolismo Secundário
Sonchus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzofurans); 0 (Herbicides); 0 (Heterocyclic Compounds, 4 or More Rings); 0 (Pyrones); 0 (colletochlorin E); 0 (colletochlorin F); LK6946W774 (benzofuran)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170602
[Lr] Data última revisão:
170602
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170103
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b05193


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[PMID]:27739871
[Au] Autor:Mishra T; Singh NB; Singh N
[Ad] Endereço:a Eco-auditing Group, CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , Uttar Pradesh , India.
[Ti] Título:Restoration of red mud deposits by naturally growing vegetation.
[So] Source:Int J Phytoremediation;19(5):439-445, 2017 May 04.
[Is] ISSN:1549-7879
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Disposal of red mud (RM) poses serious environmental problems such as wind erosion, air and water pollution. To overcome these problems, effective restoration of the disposal land through naturally growing vegetation is a sustainable and economical approach. The present study involved estimation of frequency (F), density (D), abundance (Ab), and important value index (IVI) of natural flora on abandoned RM sites in order to assess their metal toxicity tolerance capacity. Based on visual observations and highest IVI, S. Asper and S. punicea were identified as effective ecological tools for the restoration of barren RM sites. From the study, remarkable differences were observed between non-rhizospheric and rhizospheric RM of both species. These rhizospheric RM analyses confirm the ability of S. asper and S. punicea for enhancing the biological activities of abandoned RM. Translocation factor (TF) of iron was maximum (2.58) in S. asper, and bioconcentration factor (BCF) was found maximum (1.25) in S. punicea, but both TF (2.58) and BCF (1.35) were high in S. asper. Therefore, this plant could be reported as an iron hyperaccumulator plant. These results suggest that these plant species can be exploited for effective restoration of RM deposited land without any inputs or maintenance.
[Mh] Termos MeSH primário: Recuperação e Remediação Ambiental
Metais Pesados/metabolismo
Sesbania/metabolismo
Poluentes do Solo/metabolismo
Sonchus/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Índia
Resíduos Industriais/análise
Mineração
Sesbania/química
Sesbania/enzimologia
Sesbania/microbiologia
Solo/química
Sonchus/química
Sonchus/enzimologia
Sonchus/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Industrial Waste); 0 (Metals, Heavy); 0 (Soil); 0 (Soil Pollutants)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161015
[St] Status:MEDLINE
[do] DOI:10.1080/15226514.2016.1244162


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[PMID]:27707427
[Au] Autor:Ma Z; Zhao S; Cao T; Liu C; Huang Y; Gao Y; Yan K; Xiang W; Wang X
[Ad] Endereço:1​Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
[Ti] Título:Verrucosispora sonchi sp. nov., a novel endophytic actinobacterium isolated from the leaves of common sowthistle (Sonchus oleraceus L.).
[So] Source:Int J Syst Evol Microbiol;66(12):5430-5436, 2016 Dec.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel actinobacterium, designated strain NEAU-QY3T, was isolated from the leaves of Sonchus oleraceus L. and examined using a polyphasic taxonomic approach. The organism formed single spores with smooth surface on substrate mycelia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain had a close association with the genus Verrucosispora and shared the highest sequence similarity with Verrucosispora qiuiae RtIII47T (99.17 %), an association that was supported by a bootstrap value of 94 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. The strain also showed high 16S rRNA gene sequence similarities to Xiangella phaseoli NEAU-J5T (98.78 %), Jishengella endophytica 202201T (98.51 %), Micromonospora eburnea LK2-10T (98.28 %), Verrucosispora lutea YIM 013T (98.23 %) and Salinispora pacifica CNR-114T (98.23 %). Furthermore, phylogenetic analysis based on the gyrB gene sequences supported the conclusion that strain NEAU-QY3T should be assigned to the genus Verrucosispora. However, the DNA-DNA hybridization relatedness values between strain NEAU-QY3T and V. qiuiae RtIII47T and V. lutea YIM 013T were below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, strain NEAU-QY3T was readily distinguished from its most closely related strains and classified as a new species, for which the name Verrucosispora sonchi sp. nov. is proposed. The type strain is NEAU-QY3T (=CGMCC 4.7312T=DSM 101530T).
[Mh] Termos MeSH primário: Micromonosporaceae/classificação
Filogenia
Folhas de Planta/microbiologia
Sonchus/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Micromonosporaceae/genética
Micromonosporaceae/isolamento & purificação
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-39-7 (menaquinone 9); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161007
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001537


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[PMID]:27585256
[Au] Autor:Shaban NZ; Hegazy WA; Abdel-Rahman SM; Awed OM; Khalil SA
[Ad] Endereço:Alexandria University Department of Biochemistry, Faculty of Science Alexandria Egypt.
[Ti] Título:Potential effect of Olea europea leaves, Sonchus oleraceus leaves and Mangifera indica peel extracts on aromatase activity in human placental microsomes and CYP19A1 expression in MCF-7 cell line: Comparative study.
[So] Source:Cell Mol Biol (Noisy-le-grand);62(9):11-9, 2016 Aug 29.
[Is] ISSN:1165-158X
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Aromatase inhibitors (AIs) provide novel approaches to the adjuvant therapy for postmenopausal women with estrogen-receptor-positive (ER+) breast cancers. In this study, different plant extracts from Olea europaea leaves (OLE), Sonchus oleraceus L. (SOE) and Mangifera indica peels (MPE) were prepared to identify phytoconstituents and measure antioxidant capacities. The effects of these three extracts on aromatase activity in human placental microsomes were evaluated. Additionally, the effects of these extracts on tissue-specific promoter expression of CYP19A1 gene in cell culture model (MCF-7) were assessed using qRT-PCR. Results showed a concentration-dependent decrease in aromatase activity after treatment with OLE and MPE, whereas, SOE showed a biphasic effect. The differential effects of OLE, SOE and MPE on aromatase expression showed that OLE seems to be the most potent suppressor followed by SOE and then MPE. These findings indicate that OLE has effective inhibitory action on aromatase at both the enzymatic and expression levels, in addition to its cytotoxic effect against MCF-7 cells. Also, MPE may be has the potential to be used as a tissue-specific aromatase inhibitor (selective aromatase inhibitor) and it may be promising to develop a new therapeutic agent against ER+ breast cancer.
[Mh] Termos MeSH primário: Aromatase/metabolismo
Mangifera/química
Microssomos/enzimologia
Olea/química
Sonchus/química
[Mh] Termos MeSH secundário: Aromatase/genética
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Feminino
Expressão Gênica/efeitos dos fármacos
Seres Humanos
Células MCF-7
Mangifera/metabolismo
Microssomos/efeitos dos fármacos
Olea/metabolismo
Placenta/metabolismo
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Folhas de Planta/química
Folhas de Planta/metabolismo
Gravidez
Regiões Promotoras Genéticas
Reação em Cadeia da Polimerase em Tempo Real
Sonchus/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); EC 1.14.14.1 (Aromatase); EC 1.14.14.1 (CYP19A1 protein, human)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160902
[St] Status:MEDLINE


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[PMID]:27180435
[Au] Autor:Mushtaq MN; Akhtar MS; Alamgeer; Ahmad T; Khan HU; Maheen S; Ahsan H; Naz H; Asif H; Younis W; Tabassum N
[Ti] Título:EVALUATIN OF ANTIHYPERTENSIVE ACTIVITY OF SONCHUS ASPER L. IN RATS.
[So] Source:Acta Pol Pharm;73(2):425-31, 2016 Mar-Apr.
[Is] ISSN:0001-6837
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:The present investigation was carried out to evaluate the effect of aerial parts of Sonchus asper L. in normotensive, glucose and egg feed diet induced hypertensive rats. Aqueous-methanolic extract of Sonchus asper in 250, 500 and 1000 mg/kg doses was studied in normotensive and glucose induced hypertensive rats using the non-invasive technique. The results obtained showed that the extract has significantly (p < 0.5 - p < 0.001) decreased the blood pressure and heart rate in dose dependent manner. The dose 1000 mg/kg of the extract produced the maximum antihypertensive effect and was selected for further experiments. The extract was found to prevent the rise in blood pressure of egg and glucose fed rats as compared to control group in 21 days study. The LD50 of the plant extract was 3500 mg/kg b.w. in mice and sub-chronic toxicity study showed that there was no significant alteration in the blood chemistry of the extract treated rats. It is conceivable, therefore, that the aqueous-methanolic extract of Sonchus asper has exerted considerable antihypertensive activity in rats and has duly supported traditional medicinal use of plant in hypertension.
[Mh] Termos MeSH primário: Anti-Hipertensivos/farmacologia
Pressão Sanguínea/efeitos dos fármacos
Hipertensão/tratamento farmacológico
Extratos Vegetais/farmacologia
Sonchus
[Mh] Termos MeSH secundário: Animais
Anti-Hipertensivos/isolamento & purificação
Anti-Hipertensivos/toxicidade
Dieta
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Frequência Cardíaca/efeitos dos fármacos
Hipertensão/etiologia
Hipertensão/fisiopatologia
Dose Letal Mediana
Metanol/química
Camundongos
Fitoterapia
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/toxicidade
Plantas Medicinais
Ratos Sprague-Dawley
Solventes/química
Sonchus/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antihypertensive Agents); 0 (Plant Extracts); 0 (Solvents); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160516
[Lr] Data última revisão:
160516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160517
[St] Status:MEDLINE


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[PMID]:27001625
[Au] Autor:Huyan T; Li Q; Wang YL; Li J; Zhang JY; Liu YX; Shahid MR; Yang H; Li HQ
[Ad] Endereço:Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 YouyiXilu, Xi'an 710072, Shaanxi, PR China.
[Ti] Título:Anti-tumor effect of hot aqueous extracts from Sonchus oleraceus (L.) L. and Juniperus sabina L - Two traditional medicinal plants in China.
[So] Source:J Ethnopharmacol;185:289-99, 2016 Jun 05.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Sonchus oleraceus (L.) L (SO) and Juniperus sabina L (JS) are traditional medicinal plants in China. And the aqueous extracts of them have been used to treat tumor, inflammatory diseases, infection and so on in Chinese folk culture. However, the underlying mechanisms of their anti-tumor activities have not been illustrated yet. OBJECTIVE: This study aims to evaluate the inhibitory effects of aqueous extracts from SO and JS on tumor cells. MATERIALS AND METHODS: The prepared aqueous extracts of SO and JS were used to treat HepG-2 and K562 tumor cells, while the human peripheral blood mononuclear cells (PBMCs) were set as normal control. The viabilities, cell cycle and apoptosis of tumor cells after extracts treatment were assessed, in addition the expression of apoptosis-related genes (FasL, caspase 3, 6, 7, 8, 9, and 10) were analyzed. Meanwhile, the adherence and migration of HepG-2 were tested, and the expression levels of MMPs and ICAM-1 were analyzed. On top of that, the pSTAT in the two cells were also analyzed and suggested the related signaling pathway that the extracts acted on with in these tumor cells. RESULTS: Results showed that aqueous extracts of SO and JS have inhibitory effects on HepG-2 and K562 cells by decreasing cell viability and inducing apoptosis via up-regulation of the expression of the apoptosis-related genes FasL, caspase 3 and caspase 9. The extracts had different IC50 on tumor cells and PBMCs, which could block the tumor cell cycle at the G(0)/G(1) stage and significantly inhibit the adherence of HepG-2 cells. The extracts inhibited migration of these cells by inhibiting the expression of ICAM-1, MMP-2 and MMP-9. Further study indicated that the inhibition of pSTAT1 and 3 might be responsible for the inhibitory effects of the extracts on tumor cells. DISCUSSION AND CONCLUSION: The results of this study indicated that SO and JS extracts had the anti-tumor effects, which may be developed as novel anti-tumor drugs and used in cancer therapy.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Juniperus/química
Extratos Vegetais/farmacologia
Plantas Medicinais/química
Sonchus/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Apoptose
Adesão Celular/efeitos dos fármacos
Ciclo Celular/efeitos dos fármacos
Movimento Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
China
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacologia
Regulação da Expressão Gênica/efeitos dos fármacos
Células Hep G2
Temperatura Alta
Seres Humanos
Células K562
Fitoterapia
Extratos Vegetais/química
Regulação para Cima
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Drugs, Chinese Herbal); 0 (Plant Extracts); 059QF0KO0R (Water)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170224
[Lr] Data última revisão:
170224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160323
[St] Status:MEDLINE


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[PMID]:26946375
[Au] Autor:Tanvir R; Sajid I; Hasnain S; Kulik A; Grond S
[Ad] Endereço:Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Punjab, Pakistan; Institut fur Organische Chemie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 18A, 72076, Tübingen, Germany. Electronic address: rabiatanvir@hotmail.com.
[Ti] Título:Rare actinomycetes Nocardia caishijiensis and Pseudonocardia carboxydivorans as endophytes, their bioactivity and metabolites evaluation.
[So] Source:Microbiol Res;185:22-35, 2016 Apr.
[Is] ISSN:1618-0623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Two strains identified as Nocardia caishijiensis (SORS 64b) and Pseudonocardia carboxydivorans (AGLS 2) were isolated as endophytes from Sonchus oleraceus and Ageratum conyzoides respectively. The analysis of their extracts revealed them to be strongly bioactive. The N. caishijiensis extract gave an LC50 of 570 µg/ml(-1) in the brine shrimp cytotoxicity assay and an EC50 of 0.552 µg/ml(-1) in the DPPH antioxidant assay. Antimicrobial activity was observed against Methicillin resistant Staphlococcus aureus (MRSA) and Escherichia coli ATCC 25922 (14 mm), Klebsiella pneumoniae ATCC 706003 (13 mm), S. aureus ATCC 25923 (11 mm) and Candida tropicalis (20 mm). For the extract of P. carboxydivorans the EC50 was 0.670 µg/ml(-1) and it was observed to be more bioactive against Bacillus subtilis DSM 10 ATCC 6051 (21 mm), C. tropicalis (20 mm), S. aureus ATCC 25923 (17 mm), MRSA (17 mm), E. coli K12 (W1130) (16 mm) and Chlorella vulgaris (10 mm). The genotoxicity testing revealed a 20 mm zone of inhibition against the polA mutant strain E. coli K-12 AB 3027 suggesting damage to the DNA and polA genes. The TLC and bioautography screening revealed a diversity of active bands of medium polar and nonpolar compounds. Metabolite analysis by HPLC-DAD via UV/vis spectral screening suggested the possibility of stenothricin and bagremycin A in the mycelium extract of N. caishijiensis respectively. In the broth and mycelium extract of P. carboxydivorans borrelidin was suggested along with α-pyrone. The HPLC-MS revealed bioactive long chained amide derivatives such as 7-Octadecenamide, 9, 12 octadecandienamide. This study reports the rare actinomycetes N. caishijiensis and P. carboxydivorans as endophytes and evaluates their bioactive metabolites.
[Mh] Termos MeSH primário: Actinobacteria/fisiologia
Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Endófitos/fisiologia
Nocardia/fisiologia
[Mh] Termos MeSH secundário: Actinobacteria/química
Actinobacteria/isolamento & purificação
Actinobacteria/metabolismo
Ageratum/microbiologia
Aminobenzoatos/química
Animais
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Asteraceae/microbiologia
Bacillus subtilis/efeitos dos fármacos
Sequência de Bases
DNA Bacteriano/efeitos dos fármacos
Endófitos/química
Endófitos/isolamento & purificação
Endófitos/metabolismo
Escherichia coli/efeitos dos fármacos
Klebsiella pneumoniae/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Nocardia/química
Nocardia/isolamento & purificação
Nocardia/metabolismo
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência
Sonchus/microbiologia
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminobenzoates); 0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (bagremycin A)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160307
[St] Status:MEDLINE


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[PMID]:26891707
[Au] Autor:Mawalagedera SM; Ou ZQ; McDowell A; Gould KS
[Ad] Endereço:School of Biological Sciences, Victoria University of Wellington, P.O. Box 600, Wellington 6140, New Zealand. maheshinimawalagedera@yahoo.com.
[Ti] Título:Effects of boiling and in vitro gastrointestinal digestion on the antioxidant activity of Sonchus oleraceus leaves.
[So] Source:Food Funct;7(3):1515-22, 2016 Mar.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Leaves of Sonchus oleraceus L. are especially rich in phenolic compounds and have potent extractable antioxidants. However, it is not known how their antioxidant activity changes after cooking and gastrointestinal digestion. We recorded the profile of phenolics and their associated antioxidant activity in both raw and boiled S. oleraceus leaf extracts after in vitro gastric and intestinal digestion, and quantified their antioxidant potentials using Caco-2 and HepG2 cells. Boiling significantly diminished the oxygen radical absorbance capacity (ORAC) and concentrations of ascorbate and chicoric acid in the soluble fractions. In contrast, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and concentrations of caftaric and chlorogenic acids were unaffected. Phenolics in the soluble fraction were absorbed into cultured human cells and exerted antioxidant activity. Only chlorogenic acid content remained stable during gastrointestinal digestion. S. oleraceus appears to be an excellent dietary source of phenolic antioxidants.
[Mh] Termos MeSH primário: Antioxidantes/química
Digestão
Trato Gastrointestinal/metabolismo
Extratos Vegetais/química
Folhas de Planta/química
Sonchus/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Células CACO-2
Culinária
Células Hep G2
Temperatura Alta
Seres Humanos
Modelos Biológicos
Extratos Vegetais/metabolismo
Folhas de Planta/metabolismo
Sonchus/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160220
[St] Status:MEDLINE
[do] DOI:10.1039/c5fo01508a



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