Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.100.916 [Categoria DeCS]
Referências encontradas : 40 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 4 ir para página            

  1 / 40 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26711705
[Au] Autor:Matyásek R; Dobesová E; Húska D; Jezková I; Soltis PS; Soltis DE; Kovarík A
[Ad] Endereço:Laboratory of Molecular Epigenetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Brno, Czech Republic.
[Ti] Título:Interpopulation hybridization generates meiotically stable rDNA epigenetic variants in allotetraploid Tragopogon mirus.
[So] Source:Plant J;85(3):362-77, 2016 Feb.
[Is] ISSN:1365-313X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Uniparental silencing of 35S rRNA genes (rDNA), known as nucleolar dominance (ND), is common in interspecific hybrids. Allotetraploid Tragopogon mirus composed of Tragopogon dubius (d) and Tragopogon porrifolius (p) genomes shows highly variable ND. To examine the molecular basis of such variation, we studied the genetic and epigenetic features of rDNA homeologs in several lines derived from recently and independently formed natural populations. Inbred lines derived from T. mirus with a dominant d-rDNA homeolog transmitted this expression pattern over generations, which may explain why it is prevalent among natural populations. In contrast, lines derived from the p-rDNA dominant progenitor were meiotically unstable, frequently switching to co-dominance. Interpopulation crosses between progenitors displaying reciprocal ND resulted in d-rDNA dominance, indicating immediate suppression of p-homeologs in F1 hybrids. Original p-rDNA dominance was not restored in later generations, even in those segregants that inherited the corresponding parental rDNA genotype, thus indicating the generation of additional p-rDNA and d-rDNA epigenetic variants. Despite preserved intergenic spacer (IGS) structure, they showed altered cytosine methylation and chromatin condensation patterns, and a correlation between expression, hypomethylation of RNA Pol I promoters and chromatin decondensation was apparent. Reversion of such epigenetic variants occurred rarely, resulting in co-dominance maintained in individuals with distinct genotypes. Generally, interpopulation crosses may generate epialleles that are not present in natural populations, underlying epigenetic dynamics in young allopolyploids. We hypothesize that highly expressed variants with distinct IGS features may induce heritable epigenetic reprogramming of the partner rDNA arrays, harmonizing the expression of thousands of genes in allopolyploids.
[Mh] Termos MeSH primário: DNA Ribossômico/genética
Epigenômica
Evolução Molecular
Regulação da Expressão Gênica de Plantas
Genoma de Planta/genética
Tragopogon/genética
[Mh] Termos MeSH secundário: Metilação de DNA
DNA de Plantas/genética
Inativação Gênica
Genótipo
Hibridização Genética
Fenótipo
Poliploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151230
[St] Status:MEDLINE
[do] DOI:10.1111/tpj.13110


  2 / 40 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26288475
[Au] Autor:Sarac N
[Ad] Endereço:Department of Medical Services and Techniques, Vocational School of Health Sciences, Mugla Sitki Kocman University, Mugla, Turkey.
[Ti] Título:Antioxidant, mutagenic, and antimutagenic activities of Tragopogon longirostis var. longirostis, an edible wild plant in Turkey.
[So] Source:Indian J Pharmacol;47(4):414-8, 2015 Jul-Aug.
[Is] ISSN:1998-3751
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The ethanolic extract of Tragopogon longirostis var. longirostis, a wild edible plant in Anatolia was isolated, and its antioxidant, mutagenic, and antimutagenic properties were investigated. MATERIALS AND METHODS: The antioxidant activity (AA) was determined by the inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, total AA, and phenolic compounds. The mutagenic and antimutagenic activities were investigated by Ames Salmonella/microsome mutagenicity test. RESULTS: The IC50 value for DPPH radicals was 7.84 ± 0.603 mg/mL. The total AA increased with an increase in the concentration of the extracts (1, 5, 10, 20, and 30 mg/mL), containing linoleic acid emulsion. The total phenolic content was 284.71 ± 5.6 mg gallic acid equivalent/g extract. The results showed that the ethanolic extract can be considered safe, because it does not have any mutagenic effect at the tested concentrations. As a result, the ethanolic extract of the leaves exhibited antimutagenic effects at 2.5, 0.25, and 0.025 mg/plate concentrations. CONCLUSIONS: To our knowledge, this is the first study of the antioxidant, mutagenic, and antimutagenic activities of T. longirostis var. longirostis. These activities are an important topic in the food industry, as well as in the medical field.
[Mh] Termos MeSH primário: Antimutagênicos/farmacologia
Antioxidantes/farmacologia
Extratos Vegetais/farmacologia
Tragopogon/química
[Mh] Termos MeSH secundário: Antimutagênicos/administração & dosagem
Antimutagênicos/toxicidade
Antioxidantes/administração & dosagem
Antioxidantes/toxicidade
Relação Dose-Resposta a Droga
Concentração Inibidora 50
Testes de Mutagenicidade
Extratos Vegetais/administração & dosagem
Extratos Vegetais/toxicidade
Folhas de Planta
Turquia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimutagenic Agents); 0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150820
[St] Status:MEDLINE
[do] DOI:10.4103/0253-7613.161267


  3 / 40 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26101147
[Au] Autor:Granica S; Piwowarski JP; Randazzo A; Schneider P; Zyzynska-Granica B; Zidorn C
[Ad] Endereço:Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland; Institute of Pharmacy, Department of Pharmacognosy, University of Innsbruck, CCB, Innrain 80-82, Innsbruck, Austria.
[Ti] Título:Novel stilbenoids, including cannabispiradienone glycosides, from Tragopogon tommasinii (Asteraceae, Cichorieae) and their potential anti-inflammatory activity.
[So] Source:Phytochemistry;117:254-66, 2015 Sep.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A phytochemical investigation of Tragopogon tommasinii Sch.Bip. (Asteraceae, Cichorieae) yielded a total of 21 natural products, two simple phenolic acids (4-hydroxybenzoic acid and p-coumaric acid), four caffeic acid derivatives (chlorogenic acid, 3-O-caffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid), six flavonoids (luteolin, luteolin 7-O-glucoside, vitexin, orientin, quercetin 3-O-glucoside, and isorhamnetin 3-O-glucoside), three simple bibenzyls [2-carboxyl-5-hydroxy-3-methoxy-4'-ß-glucopyranosyl-oxybibenzyl, 3-caffeoyl-(9→5)-ß-apiosyl-(1→6)-ß-glucopyranosyloxy-5,4'-dihydroxy-3'-methoxybibenzyl, 3-caffeoyl-(9→5)-ß-apiosyl-(1→6)-ß-glucopyranosyloxy-4'-dihydroxy-5,3'-dimethoxybibenzyl], three phtalides [3-(4-ß-glucopyranosyloxybenzyl)-7-hydroxy-5-methoxyphtalide, 7-ß-glucopyranosyloxy-(S)-3-(4-hydroxybenzyl)-5-methoxyphtalide, and 7-(1→6)-α-rhamnosyl-ß-glucopyranosyloxy-(S)-3-(4-hydroxybenzyl)-5-methoxyphtalide], two cannabispiradienone derivatives [3-O-ß-glucopyranosyldemethoxycannabispiradienone and 3-caffeoyl-(9→5)-ß-apiosyl-(1→6)-ß-glucopyranosyloxydemethoxycannabispiradienone], and tetra-N-coumaroyl spermine. The three bibenzyls, the latter two benzylphthalides, and both cannabispiradienone derivatives represent new natural compounds and all compounds, except the caffeic acid derivatives and the flavonoids were new for T. tommasinii. The structures were established by HR mass spectrometry, extensive 1D and 2D NMR spectroscopy, and CD spectroscopy. Moreover, the potential anti-inflammatory activities of the new compounds were assayed using human neutrophils and their production of IL-1b, IL-8, TNF-α and MMP-9 as well as the expression of TLR-4, respectively.
[Mh] Termos MeSH primário: Anti-Inflamatórios/química
Anti-Inflamatórios/farmacologia
Tragopogon/química
[Mh] Termos MeSH secundário: Adulto
Células Cultivadas
Dicroísmo Circular
Avaliação Pré-Clínica de Medicamentos/métodos
Flavonoides/química
Flavonoides/farmacologia
Glucosídeos/química
Glucosídeos/farmacologia
Glicosídeos/química
Seres Humanos
Interleucina-8/metabolismo
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Neutrófilos/efeitos dos fármacos
Neutrófilos/metabolismo
Compostos de Espiro/química
Compostos de Espiro/farmacologia
Receptor 4 Toll-Like/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Flavonoids); 0 (Glucosides); 0 (Glycosides); 0 (Interleukin-8); 0 (Spiro Compounds); 0 (TLR4 protein, human); 0 (Toll-Like Receptor 4); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150828
[Lr] Data última revisão:
150828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150624
[St] Status:MEDLINE


  4 / 40 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25938765
[Au] Autor:Casseau V; De Croon G; Izzo D; Pandolfi C
[Ad] Endereço:Advanced Concepts Team, European Space Research and Technology Centre, Noordwijk, The Netherlands.
[Ti] Título:Morphologic and Aerodynamic Considerations Regarding the Plumed Seeds of Tragopogon pratensis and Their Implications for Seed Dispersal.
[So] Source:PLoS One;10(5):e0125040, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tragopogon pratensis is a small herbaceous plant that uses wind as the dispersal vector for its seeds. The seeds are attached to parachutes that increase the aerodynamic drag force and increase the total distance travelled. Our hypothesis is that evolution has carefully tuned the air permeability of the seeds to operate in the most convenient fluid dynamic regime. To achieve final permeability, the primary and secondary fibres of the pappus have evolved with complex weaving; this maximises the drag force (i.e., the drag coefficient), and the pappus operates in an "optimal" state. We used computational fluid dynamics (CFD) simulations to compute the seed drag coefficient and compare it with data obtained from drop experiments. The permeability of the parachute was estimated from microscope images. Our simulations reveal three flow regimes in which the parachute can operate according to its permeability. These flow regimes impact the stability of the parachute and its drag coefficient. From the permeability measurements and drop experiments, we show how the seeds operate very close to the optimal case. The porosity of the textile appears to be an appropriate solution to achieve a lightweight structure that allows a low terminal velocity, a stable flight and a very efficient parachute for the velocity at which it operates.
[Mh] Termos MeSH primário: Dispersão de Sementes
Sementes/anatomia & histologia
Sementes/fisiologia
Tragopogon/anatomia & histologia
Tragopogon/fisiologia
Vento
[Mh] Termos MeSH secundário: Fenômenos Biomecânicos
Hidrodinâmica
Modelos Biológicos
Permeabilidade
Porosidade
Sementes/ultraestrutura
Tragopogon/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150505
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0125040


  5 / 40 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25537492
[Au] Autor:Dobesová E; Malinská H; Matyásek R; Leitch AR; Soltis DE; Soltis PS; Kovarík A
[Ad] Endereço:Laboratory of Molecular Epigenetics, Academy of Sciences of the Czech Republic, v.v.i, Institute of Biophysics, Brno, Czech Republic.
[Ti] Título:Silenced rRNA genes are activated and substitute for partially eliminated active homeologs in the recently formed allotetraploid, Tragopogon mirus (Asteraceae).
[So] Source:Heredity (Edinb);114(3):356-65, 2015 Mar.
[Is] ISSN:1365-2540
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To study the relationship between uniparental rDNA (encoding 18S, 5.8S and 26S ribosomal RNA) silencing (nucleolar dominance) and rRNA gene dosage, we studied a recently emerged (within the last 80 years) allotetraploid Tragopogon mirus (2n=24), formed from the diploid progenitors T. dubius (2n=12, D-genome donor) and T. porrifolius (2n=12, P-genome donor). Here, we used molecular, cytogenetic and genomic approaches to analyse rRNA gene activity in two sibling T. mirus plants (33A and 33B) with widely different rRNA gene dosages. Plant 33B had ~400 rRNA genes at the D-genome locus, which is typical for T. mirus, accounting for ~25% of total rDNA. We observed characteristic expression dominance of T. dubius-origin genes in all organs. Its sister plant 33A harboured a homozygous macrodeletion that reduced the number of T. dubius-origin genes to about 70 copies (~4% of total rDNA). It showed biparental rDNA expression in root, flower and callus, but not in leaf where D-genome rDNA dominance was maintained. There was upregulation of minor rDNA variants in some tissues. The RNA polymerase I promoters of reactivated T. porrifolius-origin rRNA genes showed reduced DNA methylation, mainly at symmetrical CG and CHG nucleotide motifs. We hypothesise that active, decondensed rDNA units are most likely to be deleted via recombination. The silenced homeologs could be used as a 'first reserve' to ameliorate mutational damage and contribute to evolutionary success of polyploids. Deletion and reactivation cycles may lead to bidirectional homogenisation of rRNA arrays in the long term.
[Mh] Termos MeSH primário: Dosagem de Genes
Inativação Gênica
Genes de Plantas
Genes de RNAr
Tragopogon/genética
[Mh] Termos MeSH secundário: Metilação de DNA
Evolução Molecular
Dados de Sequência Molecular
Região Organizadora do Nucléolo
Poliploidia
Regiões Promotoras Genéticas
RNA Ribossômico/genética
RNA Ribossômico 18S/genética
RNA Ribossômico 5,8S/genética
Análise de Sequência de DNA
Deleção de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (RNA, Ribosomal); 0 (RNA, Ribosomal, 18S); 0 (RNA, Ribosomal, 5.8S); 0 (RNA, ribosomal, 26S)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141225
[St] Status:MEDLINE
[do] DOI:10.1038/hdy.2014.111


  6 / 40 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25471611
[Au] Autor:Farzaei MH; Ghasemi-Niri SF; Abdolghafari AH; Baeeri M; Khanavi M; Navaei-Nigjeh M; Abdollahi M; Rahimi R
[Ad] Endereço:Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences , Tehran , Iran .
[Ti] Título:Biochemical and histopathological evidence on the beneficial effects of Tragopogon graminifolius in TNBS-induced colitis.
[So] Source:Pharm Biol;53(3):429-36, 2015 Mar.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Tragopogon graminifolius DC. (Compositae) (TG) has been proposed as an efficacious remedy for gastrointestinal ulcers in Iranian traditional medicine. OBJECTIVE: The present study evaluates the efficacy of TG on experimental colitis and the responsible mechanisms. MATERIALS AND METHODS: After induction of IBD by 2,4,6-trinitrobenzenesulfonic acid (TNBS), rats received standardized ethanol extract of TG aerial part at 20, 30, or 50 mg/kg/d orally. After 12 d, the rats were sacrificed and the colon was removed and assessed for macroscopic and microscopic changes. Also, tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1ß), total antioxidant capacity, myeloperoxidase (MPO), and lipid peroxidation (LPO) were measured in the colon homogenate. RESULT: TG extract significantly reduced macroscopic and microscopic scores of colitis with ED50 values of 23 and 39 mg/kg, respectively. MPO was significantly reduced in all plant extract groups with an ED50 value of 41 mg/kg. The ED50 values of extract for inhibition of TNF-α and LPO were 44 and 93 mg/kg, respectively. IL-1ß significantly decreased by 50 mg/kg of TG extract (ED50 = 57 mg/kg). Total antioxidant power markedly increased by 50 mg/kg group (ED50 = 43 mg/kg). DISCUSSION: TG exhibited efficacy on TNBS-induced colitis via anti-inflammatory, immunomodulatory, antioxidant, and mucosal healing properties. CONCLUSION: TG possesses promising healing function on colitis. Clinical trials are warranted to prove its efficacy and tolerability in IBD.
[Mh] Termos MeSH primário: Colite/tratamento farmacológico
Colite/metabolismo
Extratos Vegetais/uso terapêutico
Tragopogon
Ácido Trinitrobenzenossulfônico/toxicidade
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/uso terapêutico
Colite/patologia
Peroxidação de Lipídeos/efeitos dos fármacos
Peroxidação de Lipídeos/fisiologia
Masculino
Componentes Aéreos da Planta
Extratos Vegetais/isolamento & purificação
Distribuição Aleatória
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Plant Extracts); 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150127
[Lr] Data última revisão:
150127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141205
[St] Status:MEDLINE
[do] DOI:10.3109/13880209.2014.923004


  7 / 40 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25370212
[Au] Autor:Chester M; Riley RK; Soltis PS; Soltis DE
[Ad] Endereço:Department of Biology, University of Florida, Gainesville, FL, USA.
[Ti] Título:Patterns of chromosomal variation in natural populations of the neoallotetraploid Tragopogon mirus (Asteraceae).
[So] Source:Heredity (Edinb);114(3):309-17, 2015 Mar.
[Is] ISSN:1365-2540
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cytological studies have shown many newly formed allopolyploids (neoallopolyploids) exhibit chromosomal variation as a result of meiotic irregularities, but few naturally occurring neoallopolyploids have been examined. Little is known about how long chromosomal variation may persist and how it might influence the establishment and evolution of allopolyploids in nature. In this study we assess chromosomal composition in a natural neoallotetraploid, Tragopogon mirus, and compare it with T. miscellus, which is an allotetraploid of similar age (~40 generations old). We also assess whether parental gene losses in T. mirus correlate with entire or partial chromosome losses. Of 37 T. mirus individuals that were karyotyped, 23 (62%) were chromosomally additive of the parents, whereas the remaining 14 individuals (38%) had aneuploid compositions. The proportion of additive versus aneuploid individuals differed from that found previously in T. miscellus, in which aneuploidy was more common (69%; Fisher's exact test, P=0.0033). Deviations from parental chromosome additivity within T. mirus individuals also did not reach the levels observed in T. miscellus, but similar compensated changes were observed. The loss of T. dubius-derived genes in two T. mirus individuals did not correlate with any chromosomal changes, indicating a role for smaller-scale genetic alterations. Overall, these data for T. mirus provide a second example of prolonged chromosomal instability in natural neoallopolyploid populations.
[Mh] Termos MeSH primário: Cromossomos de Plantas/genética
Genética Populacional
Poliploidia
Tragopogon/genética
[Mh] Termos MeSH secundário: DNA de Plantas/genética
Evolução Molecular
Rearranjo Gênico
Genoma de Planta
Cariótipo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141106
[St] Status:MEDLINE
[do] DOI:10.1038/hdy.2014.101


  8 / 40 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25171850
[Au] Autor:Bouhlal S; Issanchou S; Chabanet C; Nicklaus S
[Ad] Endereço:UMR6265 Centre des Sciences du Goût et de l'Alimentation, CNRS, F-21000 Dijon, France; UMR1324 Centre des Sciences du Goût et de l'Alimentation, INRA, F-21000 Dijon, France; UMR Centre des Sciences du Goût et de l'Alimentation, Université de Bourgogne, F-21000 Dijon, France.
[Ti] Título:'Just a pinch of salt'. An experimental comparison of the effect of repeated exposure and flavor-flavor learning with salt or spice on vegetable acceptance in toddlers.
[So] Source:Appetite;83:209-17, 2014 Dec.
[Is] ISSN:1095-8304
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Children's vegetable intake is below the recommended amounts. No studies to date have tested the relevance of using salt or spices to increase children's vegetable acceptance. Our objective was to compare the effect of repeated exposure (RE) and of flavor-flavor learning (FFL) on toddlers' acceptance of a non-familiar vegetable. Two unconditioned stimuli were used: salt and a salt-associated spice. Toddlers attending six nurseries were assigned to 3 groups in a between subject design. Groups were exposed 8 times to a basic salsify puree (0.2% salt w/w; RE group; n = 47), a salty salsify puree (0.5% salt w/w; FFL-Salt group; n = 54) or a spiced salsify puree (0.2% salt and 0.02% nutmeg w/w; FFL-Nutmeg group; n = 50). Acceptance (intake and liking) of the target vegetable (basic salsify puree) and of a control vegetable (carrot puree) was evaluated at pre-exposure, at each exposure of the learning period, at post-exposure, and at 1, 3 and 6 months after exposure. In all groups, intake of the target vegetable increased from pre- to post-exposure. This increase was significantly higher in the RE group (64 ± 11 g) than in the FFL-Salt group (23 ± 11 g) and marginally higher than in the FFL-Nutmeg group (36 ± 11 g). No difference between groups was observed on the increase in liking of the target vegetable from pre- to post-exposure. The increase of the target vegetable intake was still observed after 6 months for all groups. Thus, repeated exposure appears to be the simplest choice to increase vegetable intake on the short and long term in toddlers.
[Mh] Termos MeSH primário: Aprendizagem por Associação
Desenvolvimento Infantil
Fenômenos Fisiológicos da Nutrição Infantil
Dieta Hipossódica
Preferências Alimentares
Especiarias
Verduras
[Mh] Termos MeSH secundário: Pré-Escolar
Feminino
Seguimentos
França
Seres Humanos
Masculino
Myristica fragrans
Política Nutricional
Raízes de Plantas
Sementes
Método Simples-Cego
Cloreto de Sódio na Dieta/administração & dosagem
Cloreto de Sódio na Dieta/efeitos adversos
Tragopogon
[Pt] Tipo de publicação:CONTROLLED CLINICAL TRIAL; JOURNAL ARTICLE; MULTICENTER STUDY; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Sodium Chloride, Dietary)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141202
[Lr] Data última revisão:
141202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140831
[St] Status:MEDLINE


  9 / 40 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25145399
[Au] Autor:Sehrish T; Symonds VV; Soltis DE; Soltis PS; Tate JA
[Ti] Título:Gene silencing via DNA methylation in naturally occurring Tragopogon miscellus (Asteraceae) allopolyploids.
[So] Source:BMC Genomics;15:701, 2014 Aug 22.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hybridization coupled with whole-genome duplication (allopolyploidy) leads to a variety of genetic and epigenetic modifications in the resultant merged genomes. In particular, gene loss and gene silencing are commonly observed post-polyploidization. Here, we investigated DNA methylation as a potential mechanism for gene silencing in Tragopogon miscellus (Asteraceae), a recent and recurrently formed allopolyploid. This species, which also exhibits extensive gene loss, was formed from the diploids T. dubius and T. pratensis. RESULTS: Comparative bisulfite sequencing revealed CG methylation of parental homeologs for three loci (S2, S18 and TDF-44) that were previously identified as silenced in T. miscellus individuals relative to the diploid progenitors. One other locus (S3) examined did not show methylation, indicating that other transcriptional and post-transcriptional mechanisms are likely responsible for silencing that homeologous locus. CONCLUSIONS: These results indicate that Tragopogon miscellus allopolyploids employ diverse mechanisms, including DNA methylation, to respond to the potential shock of genome merger and doubling.
[Mh] Termos MeSH primário: Metilação de DNA/genética
Inativação Gênica
Poliploidia
Tragopogon/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Loci Gênicos/genética
Análise de Sequência de DNA
Sulfitos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Sulfites); OJ9787WBLU (hydrogen sulfite)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140823
[St] Status:MEDLINE
[do] DOI:10.1186/1471-2164-15-701


  10 / 40 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:24958927
[Au] Autor:Buggs RJ; Wendel JF; Doyle JJ; Soltis DE; Soltis PS; Coate JE
[Ad] Endereço:School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, UK r.buggs@qmul.ac.uk.
[Ti] Título:The legacy of diploid progenitors in allopolyploid gene expression patterns.
[So] Source:Philos Trans R Soc Lond B Biol Sci;369(1648), 2014 Aug 05.
[Is] ISSN:1471-2970
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Allopolyploidization (hybridization and whole-genome duplication) is a common phenomenon in plant evolution with immediate saltational effects on genome structure and gene expression. New technologies have allowed rapid progress over the past decade in our understanding of the consequences of allopolyploidy. A major question, raised by early pioneer of this field Leslie Gottlieb, concerned the extent to which gene expression differences among duplicate genes present in an allopolyploid are a legacy of expression differences that were already present in the progenitor diploid species. Addressing this question necessitates phylogenetically well-understood natural study systems, appropriate technology, availability of genomic resources and a suitable analytical framework, including a sufficiently detailed and generally accepted terminology. Here, we review these requirements and illustrate their application to a natural study system that Gottlieb worked on and recommended for this purpose: recent allopolyploids of Tragopogon (Asteraceae). We reanalyse recent data from this system within the conceptual framework of parental legacies on duplicate gene expression in allopolyploids. On a broader level, we highlight the intellectual connection between Gottlieb's phrasing of this issue and the more contemporary framework of cis- versus trans-regulation of duplicate gene expression in allopolyploid plants.
[Mh] Termos MeSH primário: Evolução Biológica
Regulação da Expressão Gênica de Plantas/genética
Hibridização Genética
Modelos Genéticos
Poliploidia
Tragopogon/genética
[Mh] Termos MeSH secundário: Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; REVIEW
[Em] Mês de entrada:1502
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140625
[St] Status:MEDLINE



página 1 de 4 ir para página            
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde