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Pesquisa : B01.650.940.800.575.912.250.100.958 [Categoria DeCS]
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[PMID]:28231741
[Au] Autor:Chiang YW; Yeh CF; Yen MH; Lu CY; Chiang LC; Shieh DE; Chang JS
[Ad] Endereço:* Department of Renal Care, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
[Ti] Título:Flos Farfarae Inhibits Enterovirus 71-Induced Cell Injury by Preventing Viral Replication and Structural Protein Expression.
[So] Source:Am J Chin Med;45(2):299-317, 2017.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Enterovirus 71 (EV71) infection can cause airway symptoms, brainstem encephalitis, neurogenic shock, and neurogenic pulmonary edema with high morbidity and mortality. There is no proven therapeutic modality. Flos Farfarae is the dried flower bud of Tussilago farfara L. that has been used to manage airway illnesses for thousands of years. It has neuro-protective activity and has been used to manage neuro-inflammatory diseases. However, it is unknown whether Flos Farfarae has activity against EV71-induced neuropathy. The current study used both human foreskin fibroblast (CCFS-1/KMC) and human rhabdomyosarcoma (RD) cell lines to test the hypothesis that a hot water extract of Flos Farfarae could effectively inhibit EV71 infection. The authenticity of Flos Farfarae was confirmed by HPLC-UV fingerprint. Through plaque reduction assays and flow cytometry, Flos Farfarae was found to inhibit EV71 infection ([Formula: see text]). Inhibition of viral replication and protein expression were further confirmed by reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR (qRT-PCR), and western blot, respectively. The estimated IC[Formula: see text]s were 106.3[Formula: see text][Formula: see text]g/mL in CCFS-1/KMC, and 15.0[Formula: see text][Formula: see text]g/mL in RD cells. Therefore, Flos Farfarae could be beneficial to inhibit EV71 infection by preventing viral replication and structural protein expression.
[Mh] Termos MeSH primário: Enterovirus Humano A/genética
Enterovirus Humano A/fisiologia
Fibroblastos/virologia
Expressão Gênica/efeitos dos fármacos
Fármacos Neuroprotetores
Extratos Vegetais/farmacologia
Tussilago
Proteínas Estruturais Virais/genética
Proteínas Estruturais Virais/metabolismo
Replicação Viral/efeitos dos fármacos
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Depressão Química
Relação Dose-Resposta a Droga
Enterovirus Humano A/patogenicidade
Infecções por Enterovirus/tratamento farmacológico
Prepúcio do Pênis/citologia
Células Hep G2
Seres Humanos
Masculino
Extratos Vegetais/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuroprotective Agents); 0 (Plant Extracts); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X17500197


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[PMID]:28215402
[Au] Autor:Song K; Lee KJ; Kim YS
[Ad] Endereço:College of Pharmacy, Natural Products Research Institute, Seoul National University, Seoul 08826, South Korea.
[Ti] Título:Development of an efficient fractionation method for the preparative separation of sesquiterpenoids from Tussilago farfara by counter-current chromatography.
[So] Source:J Chromatogr A;1489:107-114, 2017 Mar 17.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel application of counter-current chromatography (CCC) to enrich plant extracts using direct and continuous injection (CCC-DCI) was developed to fractionate sesquiterpenoids from the buds of Tussilago farfara L. In this study, an n-hexane-acetonitrile-water (HAcW) solvent system was separately pumped into the CCC column, and an extraction solution (45% acetonitrile) was directly and continuously injected into the CCC column. Since the extraction solution was used as a mobile phase in this method, solvent consumption could be greatly reduced. To enrich the extraction solution (315.9g/5.4L), only 4.2L water, 4.6L acetonitrile, and 1.2L n-hexane were used, including the extraction step. Finally, 6.8g of a sesquiterpenoid-enriched (STE) fraction was obtained from the crude extract (315.9g) of Tussilago farfara (1kg) in a single CCC run with a separation time of 8.5h. The sample injection capacity of CCC-DCI was greater than 300g; this amount of sample could not be handled in conventional CCC or other fractionation methods with the same column volume. Moreover, three major sesquiterpenoids (1: tussilagone, 2: 14-acetoxy-7ß-(3'-ethyl cis-crotonoyloxy)-1α-(2'-methylburyryloxy)-notonipetranone, and 3: 7ß-(3'-ethyl cis-crotonoyloxy)-1α-(2'-methylburyryloxy)-3, 14-dehydro-Z-notonipetranone) were purified from the STE fraction by CCC, and their chemical structures were elucidated by H NMR and C NMR. A quantification study was conducted, and the contents of compounds 1-3 in the CCC-DCI fraction were higher than those of conventional multi-step fractionations performed in series: solvent partitioning and open column chromatography. Furthermore, the average CCC-DCI recoveries were 96.1% (1), 96.9% (2), and 94.6% (3), whereas the open column chromatography recoveries were 77.7% (1), 66.5% (2), and 58.4% (3). The developed method demonstrates that CCC is a useful technique for enriching target components from natural products.
[Mh] Termos MeSH primário: Distribuição Contracorrente/métodos
Sesquiterpenos/isolamento & purificação
Tussilago/química
[Mh] Termos MeSH secundário: Acetonitrilos/química
Hexanos/química
Extratos Vegetais/química
Solventes/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Hexanes); 0 (Plant Extracts); 0 (Sesquiterpenes); 0 (Solvents); 059QF0KO0R (Water); 2DDG612ED8 (n-hexane); 95N0GBB2SX (tussilagone); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


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[PMID]:27150548
[Au] Autor:Muravnik LE; Kostina OV; Shavarda AL
[Ad] Endereço:Laboratory of Plant Anatomy and Morphology, Komarov Botanical Institute of Russian Academy of Sciences, Professor Popov Street, 2, 197376, St. Petersburg, Russia. LMuravnik@binran.ru.
[Ti] Título:Glandular trichomes of Tussilago Farfara (Senecioneae, Asteraceae).
[So] Source:Planta;244(3):737-52, 2016 Sep.
[Is] ISSN:1432-2048
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:MAIN CONCLUSION: The glandular trichomes are developed on the aerial organs of Tussilago farfara ; they produce phenols and terpenoids. Smooth endoplasmic reticulum and leucoplasts are the main organelles of the trichome secretory cells. The aim of this study was to characterise the morphology, anatomy, histochemistry and ultrastructure of the trichomes in Tussilago farfara as well as to identify composition of the secretory products. Structure of trichomes located on the peduncles, bracts, phyllaries, and leaves were studied by light and electron microscopy. The capitate glandular trichomes consist of a multicellular head and a biseriate long stalk. Histochemical tests and fluorescence microscopy reveal phenols and terpenoids in the head cells. During secretory stage, the head cells contain smooth and rough endoplasmic reticulum, Golgi apparatus, diversiform leucoplasts with opaque contents in lamellae, chloroplasts, mitochondria, and microbodies. In the capitate glandular trichomes of T. farfara subcuticular cavity is absent, unlike glandular trichomes in other Asteraceae species. For the first time, content of metabolites in the different vegetative and reproductive organs as well as in the isolated capitate glandular trichomes was identified by GC-MS. Forty-five compounds, including organic acids, sugars, polyols, phenolics, and terpenoids were identified. It appeared that metabolite content in the methanol extracts from peduncles, bracts and phyllaries is biochemically analogous, and similar to the metabolites from leaves, in which photosynthesis happens. At the same time, the metabolites from trichome extracts essentially differ and refer to the above-mentioned secondary substances. The study has shown that the practical value of the aerial organs of coltsfoot is provided with flavonoids produced in the capitate glandular trichomes.
[Mh] Termos MeSH primário: Tricomas/ultraestrutura
Tussilago/ultraestrutura
[Mh] Termos MeSH secundário: Metaboloma
Análise Multivariada
Tricomas/metabolismo
Tussilago/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE
[do] DOI:10.1007/s00425-016-2539-x


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[PMID]:26983826
[Au] Autor:Kang U; Park J; Han AR; Woo MH; Lee JH; Lee SK; Chang TS; Woo HA; Seo EK
[Ad] Endereço:College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Korea.
[Ti] Título:Identification of cytoprotective constituents of the flower buds of Tussilago farfara against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells.
[So] Source:Arch Pharm Res;39(4):474-80, 2016 Apr.
[Is] ISSN:0253-6269
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:A new cytoprotective compound, 1-[(4S)-3,4-dihydro-4-hydroxy-2,2-dimethyl-2H-1-benzopyran-6-yl]-ethanone (1) was isolated from the flower buds of Tussilago farfara L. (Compositae), together with eight known compounds, 3,4-dicaffeoyl isoquinic acid (2), trans-cinnamic acid (3), 4-hydroxyacetophenone (4), 4,5-dicaffeoylquinic acid methyl ester (5), 3,5-dicaffeoylquinic acid methyl ester (6), 4-hydroxybenzoic acid (7), isoquercetrin (8), and ligucyperonol (9). Compounds 2-4 were found in this plant for the first time. The isolates 1-9, were tested for their cytoprotective activities against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells. Among them, 1 and 3 showed significant cytoprotective activities as determined by MTT assay and lactate dehydrogenase leakage, indicating their possibility as the potent cytoprotective agents. The structure of 1 was determined by spectroscopic data analysis including 1D- and 2D-NMR experiments, and its absolute configuration was elucidated by a circular dichroism.
[Mh] Termos MeSH primário: Benzopiranos/isolamento & purificação
Benzopiranos/farmacologia
Citoproteção/efeitos dos fármacos
Flores/química
Glucose Oxidase/toxicidade
Queratinócitos/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Tussilago/química
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular/efeitos dos fármacos
Seres Humanos
Queratinócitos/metabolismo
Camundongos
Estrutura Molecular
Células NIH 3T3
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (1-(3,4-dihydro-4-hydroxy-2,2-dimethyl-2H-1-benzopyran-6-yl)ethanone); 0 (Benzopyrans); EC 1.1.3.4 (Glucose Oxidase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170116
[Lr] Data última revisão:
170116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160318
[St] Status:MEDLINE
[do] DOI:10.1007/s12272-016-0730-z


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[PMID]:26950613
[Au] Autor:Lee J; Kang U; Seo EK; Kim YS
[Ad] Endereço:Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
[Ti] Título:Heme oxygenase-1-mediated anti-inflammatory effects of tussilagonone on macrophages and 12-O-tetradecanoylphorbol-13-acetate-induced skin inflammation in mice.
[So] Source:Int Immunopharmacol;34:155-64, 2016 May.
[Is] ISSN:1878-1705
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The dried flower buds of Tussilago farfara L. have been used in traditional medicine, mainly as an antitussive in the treatment of cough and other respiratory problems. In the present study, we investigated the anti-inflammatory signaling pathway via the upregulation of heme oxygenase-1 (HO-1) in response to tussilagonone (TGN), a sesquiterpene compound isolated from T. farfara. TGN induced HO-1 expression and nuclear factor-E2-related factor 2 (Nrf2) activation in RAW 264.7 cells. Nuclear translocation of Nrf2 by TGN also increased in a time- and dose-dependent manner, indicating that TGN induced HO-1 via the Nrf2 pathway. Consistent with the notion that HO-1 has anti-inflammatory properties, TGN suppressed inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression and reduced the mRNA expression of proinflammatory cytokines, as well as nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. TGN inhibited the phosphorylation and degradation of inhibitory κB-α (IκB-α) and the nuclear translocation of nuclear factor (NF)-κB. However, a specific inhibitor of HO-1 reversed the TGN-mediated suppression of NO production and knockdown of HO-1 by small interfering RNA abrogated inhibitory effects of TGN on iNOS and COX-2 protein expression and NF-κB nuclear translocation. Furthermore, TGN reduced iNOS and COX-2 expression in a 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation mouse model. Taken together, these findings suggest an important role for TGN-induced HO-1 activation in regulating inflammatory responses. Moreover, TGN is a potent therapeutic candidate for targeting the crosstalk between Nrf2/HO-1 and the NF-κB signaling pathway in the prevention or treatment of inflammation-associated diseases.
[Mh] Termos MeSH primário: Anti-Inflamatórios/uso terapêutico
Dermatite/tratamento farmacológico
Heme Oxigenase-1/metabolismo
Macrófagos/efeitos dos fármacos
Fator 2 Relacionado a NF-E2/metabolismo
NF-kappa B/metabolismo
Sesquiterpenos/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Ciclo-Oxigenase 2/genética
Ciclo-Oxigenase 2/metabolismo
Feminino
Heme Oxigenase-1/genética
Seres Humanos
Lipopolissacarídeos/imunologia
Macrófagos/imunologia
Camundongos
Camundongos Endogâmicos ICR
Óxido Nítrico Sintase Tipo II/genética
Óxido Nítrico Sintase Tipo II/metabolismo
Ésteres de Forbol/administração & dosagem
RNA Interferente Pequeno/genética
Transdução de Sinais/efeitos dos fármacos
Tussilago/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Lipopolysaccharides); 0 (NF-E2-Related Factor 2); 0 (NF-kappa B); 0 (Nfe2l2 protein, mouse); 0 (Phorbol Esters); 0 (RNA, Small Interfering); 0 (Sesquiterpenes); 85527-86-2 (12-O-undecadienoylphorbol-13-acetate); 95N0GBB2SX (tussilagone); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.14.18 (Heme Oxygenase-1); EC 1.14.99.1 (Cyclooxygenase 2)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160308
[St] Status:MEDLINE


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[PMID]:26474586
[Au] Autor:Jang H; Lee JW; Lee C; Jin Q; Choi JY; Lee D; Han SB; Kim Y; Hong JT; Lee MK; Hwang BY
[Ad] Endereço:College of Pharmacy, Chungbuk National University, Cheongju, 28644, Korea.
[Ti] Título:Sesquiterpenoids from Tussilago farfara inhibit LPS-induced nitric oxide production in macrophage RAW 264.7 cells.
[So] Source:Arch Pharm Res;39(1):127-32, 2016 Jan.
[Is] ISSN:0253-6269
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:A new oplopane-type sesquiterpenoid tussilagofarin (1) and a new chromone tussilagofarol (2), along with 18 known compounds 3-20, were isolated from the flower buds of Tussilago farfara. The structures of the new compounds were elucidated on the basis of 1D and 2D NMR and HRESIMS data. Of the isolated compounds, oplopane- and bisabolane-type sesquterpenoids 1, 8-12, 15, and 16 were found to inhibit nitric oxide production in LPS-induced RAW 264.7 cells with IC50 values of 3.5-28.5 µM.
[Mh] Termos MeSH primário: Lipopolissacarídeos/antagonistas & inibidores
Macrófagos/efeitos dos fármacos
Óxido Nítrico/antagonistas & inibidores
Sesquiterpenos/isolamento & purificação
Sesquiterpenos/farmacologia
Tussilago
[Mh] Termos MeSH secundário: Animais
Flores
Lipopolissacarídeos/toxicidade
Macrófagos/metabolismo
Camundongos
Óxido Nítrico/biossíntese
Células RAW 264.7
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lipopolysaccharides); 0 (Sesquiterpenes); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151018
[St] Status:MEDLINE
[do] DOI:10.1007/s12272-015-0667-7


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[PMID]:26439905
[Au] Autor:Wu QZ; Zhao DX; Xiang J; Zhang M; Zhang CF; Xu XH
[Ad] Endereço:a Research Department of Pharmacognosy , China Pharmaceutical University , Nanjing , China.
[Ti] Título:Antitussive, expectorant, and anti-inflammatory activities of four caffeoylquinic acids isolated from Tussilago farfara.
[So] Source:Pharm Biol;54(7):1117-24, 2016 Jul.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: The flower bud of Tussilago farfara L. (Compositae) (FTF) is one of the traditional Chinese medicinal herbs used to treat cough, phlegm, bronchitic, and asthmatic conditions. OBJECTIVE: The objective of this study is to isolate four caffeoylquinic acids from the ethyl acetate extract (EtE) of FTF and to evaluate their antitussive, expectorant, and anti-inflammatory activities. MATERIALS AND METHODS: The structures of compounds 1-4 isolated from EtE were determined by spectral analysis. Mice were orally treated with these compounds and their mixture (in a ratio of 5:28:41:26 as in EtE) at doses of 10 and 20 mg/kg once daily for 3 d. The antitussive and expectorant activities were evaluated separately with the ammonia liquor-induced model and the phenol red secretion model. The anti-inflammation activity was evaluated using leukocyte count in the bronchoalveolar lavage fluid after ammonia liquor-induced acute airway inflammation. RESULTS: The four compounds were identified as chlorogenic acid (1), 3,5-dicaffeoylquinic acid (2), 3,4-dicaffeoylquinic acid (3), and 4,5-dicaffeoylquinic acid (4). All compounds, especially compound 4 (58.0% inhibition in cough frequency), showed a significant antitussive effect. However, the mixture was the most effective to inhibit the cough frequency by 61.7%. All compounds also showed a significant expectorant effect, while compound 2 was the most potent to enhance the phenol red secretion by 35.7%. All compounds significantly alleviated inflammation, but compound 4 showed the strongest effect to inhibit the leukocytosis by 49.7%. DISCUSSION AND CONCLUSION: The caffeoylquinic acids and their mixture, exhibiting significant antitussive, expectorant, and anti-inflammatory effects, could be considered as the main effective ingredients of FTF, and they may act in a collective and synergistic way.
[Mh] Termos MeSH primário: Antitussígenos/farmacologia
Tosse/prevenção & controle
Expectorantes/farmacologia
Extratos Vegetais/farmacologia
Pneumonia/prevenção & controle
Ácido Quínico/análogos & derivados
[Mh] Termos MeSH secundário: Acetatos/química
Amônia
Animais
Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/farmacologia
Antitussígenos/isolamento & purificação
Tosse/induzido quimicamente
Tosse/imunologia
Modelos Animais de Doenças
Expectorantes/isolamento & purificação
Flores
Leucocitose/induzido quimicamente
Leucocitose/prevenção & controle
Camundongos Endogâmicos ICR
Fenolsulfonaftaleína
Fitoterapia
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Pneumonia/induzido quimicamente
Pneumonia/imunologia
Ácido Quínico/isolamento & purificação
Ácido Quínico/farmacologia
Solventes/química
Tussilago/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Anti-Inflammatory Agents); 0 (Antitussive Agents); 0 (Expectorants); 0 (Plant Extracts); 0 (Solvents); 0 (caffeoylquinic acid); 058C04BGYI (Quinic Acid); 7664-41-7 (Ammonia); 76845O8NMZ (ethyl acetate); I6G9Y0J1OJ (Phenolsulfonphthalein)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151007
[St] Status:MEDLINE
[do] DOI:10.3109/13880209.2015.1075048


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[PMID]:26234144
[Au] Autor:Zhang ZZ; Zhi HJ; Qin XM; Li ZY
[Ti] Título:[Chemical comparison of different Farfarae Flos by NMR-based metabolomic approaches].
[So] Source:Yao Xue Xue Bao;50(5):599-604, 2015 May.
[Is] ISSN:0513-4870
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:1H NMR-based metabolomic approach combined with multivariate statistical analysis was used to evaluate the quality of 21 Farfarae Flos (FF) samples from different growth regions. Principal component analysis showed that wild and cultivated FF could be separated clearly, suggesting a big chemical difference existed between them. Supervised PLS-DA analysis indicated that the wild samples showed higher levels of secondary metabolites, such as bauer-7-ene-3ß, 16α-diol, chlorogenic acid, rutin, 7-(3'-ethylcrotonoyloxy)-1α-(2'-methyl-butyryloxy)-3, 14-dehydro-Z-notonipetranone (EMDNT), tussilagone, ß-sitosterol and sitosterone. This is consistent with traditional experience that the quality of wild samples are better than that of cultivated ones. The content of pyrrolizidine alkaloids senkirkine also differed greatly among samples from different habitats. The Pearson correlation analysis showed that senkirkine is positively correlated with 4, 5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, rutin, kampferol analogues, to a statistically significant extent. The correlation between the toxic compounds and the bioactive components in FF should be further studied.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/química
Flores/química
Metabolômica
Tussilago/química
[Mh] Termos MeSH secundário: Ácido Clorogênico
Imagem por Ressonância Magnética
Espectroscopia de Ressonância Magnética
Ácido Quínico/análogos & derivados
Rutina
Sitosteroides
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Sitosterols); 0 (caffeoylquinic acid); 058C04BGYI (Quinic Acid); 318ADP12RI (Chlorogenic Acid); 5G06TVY3R7 (Rutin); 5LI01C78DD (gamma-sitosterol)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150803
[Lr] Data última revisão:
150803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150804
[St] Status:MEDLINE


  9 / 44 MEDLINE  
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[PMID]:25971744
[Au] Autor:Seo UM; Zhao BT; Kim WI; Seo EK; Lee JH; Min BS; Shin BS; Son JK; Woo MH
[Ad] Endereço:College of Pharmacy, Catholic University of Daegu.
[Ti] Título:Quality evaluation and pattern recognition analyses of bioactive marker compounds from Farfarae Flos using HPLC/PDA.
[So] Source:Chem Pharm Bull (Tokyo);63(7):546-53, 2015.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The flower bud of Tussilago farfara L., called Farfarae Flos, has traditionally been used in Oriental medicine for the treatment of bronchitis and asthma. To establish a standard for quality control as well as the reliable identification of Farfarae Flos, the contents of five standards, rutin (1), isoquercetin (2), 3,5-dicaffeoylquinic acid (3), tussilagone (4), and tussilagonone (5), were determined by quantitative high-performance liquid chromatography (HPLC)/photodiode array (PDA) analysis. The five standards were separated on a YoungJinBioChrom Aegispak C18-L (250-mm×4.6-mm, 5-µm) column by gradient elution using 0.03% trifluoroacetic acid in water (A), with acetonitrile (B) as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 220 nm. The method was successfully used in the analysis of Farfarae Flos from different geographic origins with relatively simple conditions and procedures, and the results demonstrated satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 62 Farfarae Flos samples. This result indicated that the established HPLC/PDA method is suitable for quantitation and pattern recognition analyses for the quality evaluation of Farfarae Flos.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão
Extratos Vegetais/análise
Tussilago/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/normas
Análise por Conglomerados
Flores/química
Flores/metabolismo
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Controle de Qualidade
Tussilago/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Extracts)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150703
[Lr] Data última revisão:
150703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150515
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c15-00204


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[PMID]:25459951
[Au] Autor:Li ZY; Zhang SS; Jie-Xing; Qin XM
[Ad] Endereço:Modern Research Center for Traditional Chinese Medicine of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People's Republic of China. Electronic address: lizhenyu@sxu.eud.cn.
[Ti] Título:Effect of lithium salts addition on the ionic liquid based extraction of essential oil from Farfarae Flos.
[So] Source:J Pharm Biomed Anal;102:509-13, 2015 Jan.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, an ionic liquids (ILs) based extraction approach has been successfully applied to the extraction of essential oil from Farfarae Flos, and the effect of lithium chloride was also investigated. The results indicated that the oil yields can be increased by the ILs, and the extraction time can be reduced significantly (from 4h to 2h), compared with the conventional water distillation. The addition of lithium chloride showed different effect according to the structures of ILs, and the oil yields may be related with the structure of cation, while the chemical compositions of essential oil may be related with the anion. The reduction of extraction time and remarkable higher efficiency (5.41-62.17% improved) by combination of lithium salt and proper ILs supports the suitability of the proposed approach.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/análise
Líquidos Iônicos/análise
Extração Líquido-Líquido/métodos
Compostos de Lítio/análise
Óleos Voláteis/análise
Tussilago
[Mh] Termos MeSH secundário: Medicamentos de Ervas Chinesas/química
Flores
Química Verde/métodos
Líquidos Iônicos/química
Compostos de Lítio/química
Óleos Voláteis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Ionic Liquids); 0 (Lithium Compounds); 0 (Oils, Volatile)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:150202
[Lr] Data última revisão:
150202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE



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