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Pesquisa : B01.650.940.800.575.912.250.106 [Categoria DeCS]
Referências encontradas : 35 [refinar]
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[PMID]:28694126
[Au] Autor:Wei J; Huo X; Yu Z; Tian X; Deng S; Sun C; Feng L; Wang C; Ma X; Jia J
[Ad] Endereço:School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China; College of Pharmacy, Academy of Integrative Medicine, Liaoning Engineering Technology Centre of Target-based Nature Products for Prevention and Treatment of Ageing-related Neurodegeneration,
[Ti] Título:Phenolic acids from Balanophora involucrata and their bioactivities.
[So] Source:Fitoterapia;121:129-135, 2017 Sep.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The bioactive substance investigation of Balanophora involucrata obtained 15 phenolic acids, including 5 new compounds (1-3, 8, 9), which were determined by various spectroscopic data analyses. Most isolated compounds displayed inhibitory effects on α-Glucosidase in vitro. For the potential inhibitors 8 (1.95µM) and 10 (9.02µM), the inhibition kinetics have been studied, which gave the K values as 0.68, 3.15µM respectively. And, in silico docking analyses have been performed to investigate the inhibitory mechanism of compounds 8 and 10. Additionally, most isolated compounds showed anti-oxidant activities in the DPPH scavenging assay. New compound 8 also could inhibit the acetyl transfer activity of GlmU moderately with the IC value of 18.21µM, which was a new antibacterial target.
[Mh] Termos MeSH primário: Balanophoraceae/química
Depuradores de Radicais Livres/química
Inibidores de Glicosídeo Hidrolases/química
Hidroxibenzoatos/química
[Mh] Termos MeSH secundário: Depuradores de Radicais Livres/isolamento & purificação
Inibidores de Glicosídeo Hidrolases/isolamento & purificação
Hidroxibenzoatos/isolamento & purificação
Simulação de Acoplamento Molecular
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Free Radical Scavengers); 0 (Glycoside Hydrolase Inhibitors); 0 (Hydroxybenzoates); 0 (Plant Extracts); 29656-58-4 (phenolic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE


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[PMID]:23776023
[Au] Autor:She GM; Zhang YJ; Yang CR
[Ad] Endereço:State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
[Ti] Título:A new phenolic constituent and a cyanogenic glycoside from Balanophora involucrata (Balanophoraceae).
[So] Source:Chem Biodivers;10(6):1081-7, 2013 Jun.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Balanophora involucrata HOOK.f. & THOMSON (Balanophoraceae) is a parasite plant often growing on the roots of leguminous plants. The whole herb has been used medicinally for the treatment of irregular menstruation, cough, hemoptysis, traumatic injury and bleeding, dizziness and gastralgia in Yunnan Province, China. The 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay on the 60% aq. acetone extract of the fresh whole plant of B. involucrata showed considerable radical-scavenging activity (SC50 15.3 µg/ml). Further purification on the extract led to the isolation of one new phenolic glycoside, sieboldin-3'-ketocarboxylic acid (1), and one new cyanogenic glycoside, proacacipetalin 6'-O-ß-D-glucopyranoside (2), together with 26 known compounds including three 4"-O-galloyl and 2",3"-O-(S)-hexahydroxydiphenoyl (HHDP) derivatives of dihydrochalcone glucosides, seven hydrolyzable tannins, and alkane glycosides. The cyanogenic compound isolated from the Balanophoraceae family for the first time might be a signal molecule between B. involucrata and its hosts. The free-radical-scavenging activity of the isolated compounds was also examined by DPPH assay.
[Mh] Termos MeSH primário: Balanophoraceae/química
Depuradores de Radicais Livres/química
Glicosídeos/química
Fenóis/química
[Mh] Termos MeSH secundário: Depuradores de Radicais Livres/isolamento & purificação
Glicosídeos/isolamento & purificação
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Free Radical Scavengers); 0 (Glycosides); 0 (Phenols)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:130618
[Lr] Data última revisão:
130618
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130619
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201200103


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[PMID]:23041381
[Au] Autor:Su HJ; Hu JM
[Ad] Endereço:Institute of Ecology and Evolutionary Biology, National Taiwan University, Taiwan.
[Ti] Título:Rate heterogeneity in six protein-coding genes from the holoparasite Balanophora (Balanophoraceae) and other taxa of Santalales.
[So] Source:Ann Bot;110(6):1137-47, 2012 Nov.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: The holoparasitic flowering plant Balanophora displays extreme floral reduction and was previously found to have enormous rate acceleration in the nuclear 18S rDNA region. So far, it remains unclear whether non-ribosomal, protein-coding genes of Balanophora also evolve in an accelerated fashion and whether the genes with high substitution rates retain their functionality. To tackle these issues, six different genes were sequenced from two Balanophora species and their rate variation and expression patterns were examined. METHODS: Sequences including nuclear PI, euAP3, TM6, LFY and RPB2 and mitochondrial matR were determined from two Balanophora spp. and compared with selected hemiparasitic species of Santalales and autotrophic core eudicots. Gene expression was detected for the six protein-coding genes and the expression patterns of the three B-class genes (PI, AP3 and TM6) were further examined across different organs of B. laxiflora using RT-PCR. KEY RESULTS: Balanophora mitochondrial matR is highly accelerated in both nonsynonymous (d(N)) and synonymous (d(S)) substitution rates, whereas the rate variation of nuclear genes LFY, PI, euAP3, TM6 and RPB2 are less dramatic. Significant d(S) increases were detected in Balanophora PI, TM6, RPB2 and d(N) accelerations in euAP3. All of the protein-coding genes are expressed in inflorescences, indicative of their functionality. PI is restrictively expressed in tepals, synandria and floral bracts, whereas AP3 and TM6 are widely expressed in both male and female inflorescences. CONCLUSIONS: Despite the observation that rates of sequence evolution are generally higher in Balanophora than in hemiparasitic species of Santalales and autotrophic core eudicots, the five nuclear protein-coding genes are functional and are evolving at a much slower rate than 18S rDNA. The mechanism or mechanisms responsible for rapid sequence evolution and concomitant rate acceleration for 18S rDNA and matR are currently not well understood and require further study in Balanophora and other holoparasites.
[Mh] Termos MeSH primário: Balanophoraceae/genética
Evolução Molecular
Flores/genética
Filogenia
Proteínas de Plantas/genética
Santalaceae/genética
[Mh] Termos MeSH secundário: Balanophoraceae/ultraestrutura
DNA Mitocondrial/química
DNA Mitocondrial/genética
DNA de Plantas/química
DNA de Plantas/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Flores/ultraestrutura
Inflorescência/genética
Inflorescência/ultraestrutura
Microscopia Eletrônica de Varredura
Mitocôndrias/genética
Proteínas Nucleares/genética
Epiderme Vegetal/genética
Epiderme Vegetal/ultraestrutura
RNA Ribossômico 18S/genética
Santalaceae/ultraestrutura
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (DNA, Plant); 0 (DNA, Ribosomal); 0 (Nuclear Proteins); 0 (Plant Proteins); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121009
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcs197


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[PMID]:23025275
[Au] Autor:Wood JR; Wilmshurst JM; Worthy TH; Holzapfel AS; Cooper A
[Ad] Endereço:Landcare Research, P.O. Box 40, Lincoln, Canterbury 7640, New Zealand. woodj@landcareresearch.co.nz
[Ti] Título:A lost link between a flightless parrot and a parasitic plant and the potential role of coprolites in conservation paleobiology.
[So] Source:Conserv Biol;26(6):1091-9, 2012 Dec.
[Is] ISSN:1523-1739
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Late Quaternary extinctions and population fragmentations have severely disrupted animal-plant interactions globally. Detection of disrupted interactions often relies on anachronistic plant characteristics, such as spines in the absence of large herbivores or large fruit without dispersers. However, obvious anachronisms are relatively uncommon, and it can be difficult to prove a direct link between the anachronism and a particular faunal taxon. Analysis of coprolites (fossil feces) provides a novel way of exposing lost interactions between animals (depositors) and consumed organisms. We analyzed ancient DNA to show that a coprolite from the South Island of New Zealand was deposited by the rare and threatened kakapo (Strigops habroptilus), a large, nocturnal, flightless parrot. When we analyzed the pollen and spore content of the coprolite, we found pollen from the cryptic root-parasite Dactylanthus taylorii. The relatively high abundance (8.9% of total pollen and spores) of this zoophilous pollen type in the coprolite supports the hypothesis of a former direct feeding interaction between kakapo and D. taylorii. The ranges of both species have contracted substantially since human settlement, and their present distributions no longer overlap. Currently, the lesser short-tailed bat (Mystacina tuberculata) is the only known native pollinator of D. taylorii, but our finding raises the possibility that birds, and other small fauna, could have once fed on and pollinated the plant. If confirmed, through experimental work and observations, this finding may inform conservation of the plant. For example, it may be possible to translocate D. taylorii to predator-free offshore islands that lack bats but have thriving populations of endemic nectar-feeding birds. The study of coprolites of rare or extinct taxonomic groups provides a unique way forward to expand existing knowledge of lost plant and animal interactions and to identify pollination and dispersal syndromes. This approach of linking paleobiology with neoecology offers significant untapped potential to help inform conservation and restoration plans.
[Mh] Termos MeSH primário: Balanophoraceae/fisiologia
Conservação dos Recursos Naturais
Espécies em Perigo de Extinção
Cadeia Alimentar
Fósseis
Papagaios/fisiologia
[Mh] Termos MeSH secundário: Animais
DNA/análise
Dieta
Fezes/química
Nova Zelândia
Dispersão Vegetal
Pólen/química
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:121108
[Lr] Data última revisão:
121108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121003
[St] Status:MEDLINE
[do] DOI:10.1111/j.1523-1739.2012.01931.x


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[PMID]:22909424
[Au] Autor:Tao J; Zhao J; Zhao Y; Cui Y; Fang W
[Ad] Endereço:State Key Laboratory of Bioactive Substances and Functions of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
[Ti] Título:BACE inhibitory flavanones from Balanophora involucrata Hook. f.
[So] Source:Fitoterapia;83(8):1386-90, 2012 Dec.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:One new flavanone (S)-5,7,3',5'-tetrahydroxy-flavanone-7-O-(6″-galloyl)-ß-D-glucopyranose (1), together with one known flavanone and four known dihydrochalcones were isolated from the ethyl acetate partitions from an alcoholic extract of the whole plant of Balanophora involucrata Hook. f. All of the compounds were characterized by spectroscopic methods. Their in vitro BACE inhibitory effects were evaluated. Compounds 1, 2, 4, and 5 were found to be a little more active than the positive control, although all of which are below 50% inhibition at 10µM.
[Mh] Termos MeSH primário: Balanophoraceae/química
Flavanonas/farmacologia
[Mh] Termos MeSH secundário: Chalconas/química
Flavanonas/química
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 ((S)-5,7,3',5'-tetrahydroxy-flavanone-7-O-(6''-galloyl)-beta-D-glucopyranose); 0 (Chalcones); 0 (Flavanones)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:121203
[Lr] Data última revisão:
121203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120823
[St] Status:MEDLINE


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[PMID]:22837411
[Au] Autor:McLay TG; Tate JA; Symonds VV
[Ad] Endereço:Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand.
[Ti] Título:Microsatellite markers for the endangered root holoparasite Dactylanthus taylorii (Balanophoraceae) from 454 pyrosequencing.
[So] Source:Am J Bot;99(8):e323-5, 2012 Aug.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Microsatellite loci were isolated and developed as polymorphic markers for the New Zealand endemic root holoparasite Dactylanthus taylorii for use in population and conservation genetics studies. METHODS AND RESULTS: Shotgun 454 pyrosequencing was performed on genomic DNA pooled from three individuals of D. taylorii. From 61709 individual sequence reads, primers for 753 microsatellite loci were developed in silico and 72 of these were tested for consistent amplification and variability. Ten microsatellite loci were found to be polymorphic and consistently scorable when screened in 44 individuals from five geographically distant populations. The number of alleles per locus ranged from four to 16 with an average of 9.7, and average observed heterozygosity per locus was between 0.182 and 0.634. CONCLUSIONS: These polymorphic microsatellite markers establish an important resource for ongoing conservation initiatives and planned population genetic studies of D. taylorii.
[Mh] Termos MeSH primário: Balanophoraceae/genética
Primers do DNA/genética
Repetições de Microssatélites/genética
Polimorfismo Genético
[Mh] Termos MeSH secundário: Alelos
Balanophoraceae/classificação
Sequência de Bases
DNA de Plantas/genética
Loci Gênicos
Marcadores Genéticos
Sequenciamento de Nucleotídeos em Larga Escala
Inflorescência/classificação
Inflorescência/genética
Dados de Sequência Molecular
Análise de Sequência de DNA
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Plant); 0 (Genetic Markers)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:120809
[Lr] Data última revisão:
120809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120728
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1200013


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[PMID]:22428237
[Au] Autor:Bracci A; Amat AG; Maione F; Cicala C; Mascolo N; De Feo V
[Ad] Endereço:Dipartimento di Scienze Farmaceutiche e Biomediche, Università degli Studi di Salerno, Via Ponte don Melillo, 84084 Fisciano (Salerno), Italy.
[Ti] Título:Diuretic activity of Lophophytum leandri.
[So] Source:Nat Prod Commun;7(1):33-4, 2012 Jan.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A bioassay-oriented study was carried-out in order to validate the traditional uses of Lophophytum leandri, a parasitic plant used as a diuretic in traditional medical practices of Argentina. Four known flavonoids have been isolated from the active fraction. Quercetin-3-O-beta-D-glucopyranoside was identified as the active principle of the fraction. However, the diuretic activity of the extract and of the most active fraction had greater activity than that of the pure isolated compounds.
[Mh] Termos MeSH primário: Balanophoraceae
Diuréticos/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Balanophoraceae/química
Masculino
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diuretics); 0 (Plant Extracts)
[Em] Mês de entrada:1205
[Cu] Atualização por classe:120320
[Lr] Data última revisão:
120320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120321
[St] Status:MEDLINE


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[PMID]:21894574
[Au] Autor:Su HJ; Murata J; Hu JM
[Ad] Endereço:Institute of Ecology and Evolutionary Biology, National Taiwan University, Rm 1227, Life Science Building, 1 Sec. 4, Roosevelt Road, Taipei 106, Taiwan.
[Ti] Título:Morphology and phylogenetics of two holoparasitic plants, Balanophora japonica and Balanophora yakushimensis (Balanophoraceae), and their hosts in Taiwan and Japan.
[So] Source:J Plant Res;125(3):317-26, 2012 May.
[Is] ISSN:1618-0860
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Balanophora japonica and B. yakushimensis are two putatively agamospermic taxa previously reported from southern Japan. Their inflorescences superficially represent those of B. laxiflora and B. fungosa. In this study we confirmed their presence in Taiwan by morphological and phylogenetic analysis using nuclear 18S rDNA and nrITS sequences with related taxa. B. japonica, B. yakushimensis, and B. laxiflora formed a well-supported clade that is distinct from other Balanophora. All three taxa also show considerable differences on morphological and nucleotide sequence differences, therefore the name of B. yakushimensis is retained. The results provide new insights on the intrageneric classification of Balanophora and suggest the positioning of female flowers should be down-weighted. We also successfully identify the hosts of B. japonica and B. yakushimensis by amplifying chloroplast matK sequences from the connected root tissues. The results showed that B. japonica parasitizes on Symplocos species, and that B. yakushimensis parasitizes on Distylium racemosum in Japan and Schima superba in Taiwan's population.
[Mh] Termos MeSH primário: Balanophoraceae/classificação
Balanophoraceae/genética
Flores/anatomia & histologia
Filogenia
[Mh] Termos MeSH secundário: DNA de Plantas
DNA Ribossômico
Especificidade de Hospedeiro
Japão
Doenças das Plantas
Análise de Sequência de DNA
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1208
[Cu] Atualização por classe:171014
[Lr] Data última revisão:
171014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110907
[St] Status:MEDLINE
[do] DOI:10.1007/s10265-011-0447-5


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[PMID]:22506400
[Au] Autor:Ai RT; Wu SY; Wen XY; Xu W; Lv L; Wu SG
[Ad] Endereço:Southern Medical University, Guangzhou 510515, China. airt@cncbd.org.cn
[Ti] Título:[A new natural polyphenol BJA32531 inhibited the proliferation and regulated miRNA expression in human HepG2 hepatocarcinoma cells].
[So] Source:Zhong Yao Cai;34(11):1734-40, 2011 Nov.
[Is] ISSN:1001-4454
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To study the effect of 1,2,6-Tri-O-galloyl-beta-D-glucopyranose (BJA32531) on the miRNA expression during BJA32531-induced cytotoxicity in human HepG2 hepatocarcinoma cells. METHODS: Cell proliferation was assessed using a colorimetric assay (cell counting kit-8). Apoptosis was assessed by annexin V and propidium iodide staining. The miRNA expression profile of the cancer cells was analyzed by a miRNA array and quantitative real-time PCR. RESULTS: BJA32531 inhibited the cell proliferation and increased apoptosis in HepG2 cancer cells. Cellular exposure to BJA32531 influenced the miRNA expression pattern in the cells, including 19 upregulated and 85 down-regulated miRNAs in the cells. The up-regulations of let-7a and miR-10b as well as the down-regulations of miR-132 and miR-125b were verified to be consistent with the the results of the miRNA array. CONCLUSION: Our study suggests that the mechanisms by which BJA32531 exerted the antiproliferative effects on HepG2 cancer cells may be related to its regulation of miRNA.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Proliferação Celular/efeitos dos fármacos
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
MicroRNAs/metabolismo
Polifenóis/farmacologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Balanophoraceae/química
Carcinoma Hepatocelular/metabolismo
Carcinoma Hepatocelular/patologia
Relação Dose-Resposta a Droga
Citometria de Fluxo
Células Hep G2
Seres Humanos
Neoplasias Hepáticas/metabolismo
Neoplasias Hepáticas/patologia
MicroRNAs/genética
Estrutura Molecular
Análise de Sequência com Séries de Oligonucleotídeos
Polifenóis/química
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Fatores de Tempo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (MicroRNAs); 0 (Polyphenols)
[Em] Mês de entrada:1209
[Cu] Atualização por classe:120417
[Lr] Data última revisão:
120417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120418
[St] Status:MEDLINE


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[PMID]:22027761
[Au] Autor:Ai RT; Wu SY; Wen XY; Xu W; Lv L; Rao JJ; Wu SG
[Ad] Endereço:School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China. airt@cncbd.org.cn
[Ti] Título:1,3,4-tri-O-galloyl-6-O-caffeoyl-ß-D-glucopyranose, a new anti-proliferative ellagitannin, regulates the expression of microRNAs in HepG(2) cancer cells.
[So] Source:Nan Fang Yi Ke Da Xue Xue Bao;31(10):1641-8, 2011 Oct.
[Is] ISSN:1673-4254
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: MicroRNAs (miRNAs) play important roles in cell proliferation, differentiation and apoptosis. 1, 3, 4-tri-O-galloyl-6-O-caffeoyl-ß-D-glucopyranose (BJA32515) is a new natural ellagitannin compound extracted from Balanophora Japonica MAKINO. The effect of BJA32515 on the expression of miRNAs in cancer cells has not yet been explored. Objective The present study was carried out to examine the changes in miRNA expression profiles in human HepG(2) hepatocarcinoma cells following BJA32515 exposure. METHODS: The proliferation of BJA32515-exposed HepG(2) cells was assessed using a colorimetric assay (cell counting kit-8). The miRNA expression profile of the cancer cells was analyzed using a miRNA array and quantitative real-time PCR. Apoptosis was assessed by annexin V and propidium iodide staining. RESULTS: BJA32515 inhibited the cell proliferation and increased apoptosis in HepG(2) cancer cells. The exposure to BJA32515 also caused alterations in the miRNA expression profile in the cells, with 33 miRNAs upregulated and 59 down-regulated. The up-regulation of let-7a and miR-29a and the down-regulation of miR-373 and miR-197 were verified by quantitative real-time PCR. CONCLSION: BJA32515-modifed miRNA expression may mediate the antiproliferative effect of this compound in HepG(2) cancer cells.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Balanophoraceae/química
Ácidos Cafeicos/farmacologia
Glucosídeos/farmacologia
Taninos Hidrolisáveis/farmacologia
MicroRNAs/metabolismo
[Mh] Termos MeSH secundário: Antineoplásicos/farmacologia
Ácidos Cafeicos/isolamento & purificação
Proliferação Celular
Regulação Neoplásica da Expressão Gênica
Glucosídeos/isolamento & purificação
Células Hep G2
Seres Humanos
Taninos Hidrolisáveis/isolamento & purificação
MicroRNAs/genética
Polifenóis
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (1,3,4-tri-O-galloyl-6-O-caffeoyl-beta-D-glucopyranose); 0 (Antineoplastic Agents); 0 (Caffeic Acids); 0 (Glucosides); 0 (Hydrolyzable Tannins); 0 (MicroRNAs); 0 (Polyphenols); 0 (ellagitannin)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:111026
[Lr] Data última revisão:
111026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111027
[St] Status:MEDLINE



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