Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.125.311 [Categoria DeCS]
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[PMID]:25982196
[Au] Autor:Xia YG; Liang J; Li GY; Yang BY; Kuang HX
[Ad] Endereço:Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, PR China.
[Ti] Título:Analysis of oligosaccharide sequences of trace Caulophyllum robustum saponins by direct infusion multiple-stage tandem mass spectrometry.
[So] Source:J Pharm Biomed Anal;112:106-15, 2015 Aug 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The saponins in Caulophyllum robustum have not yet been fully characterized. Furthermore these saponins are often present in trace amounts and are structurally complex. Here, a simple direct infusion electrospray ion trap multiple-stage tandem mass spectrometry (DI-ESI-IT-MS(n)) method was described for the characterization of trace C. robustum saponins. Eight reference saponins from the C. robustum hairy root were investigated by DI-ESI-IT-MS(n) in positive ion mode. Some fragmentation approaches were proposed through analysis of the [M+Na](+) ions: (1) preferential cleavage of the C-28 ester glycosidic bond to provide complementary [Y0α+Na](+) and [Bα+Na](+) ions for bidesmosidic saponins; (2) diagnostically neutral loss of CO2 from free carboxyl groups at C-28 for monodesmosidic saponins; and (3) the ion intensity ratio between [C2ß+Na](+) and [B2ß+Na](+), which is sensitive to the structural differences between the two isomeric ß-sugar chains (Glc → (2)Ara and Glc → (3)Ara). The DI-ESI-IT-MS(n) method was successfully used for the analysis of trace C. robustum saponins with [M+Na](+) ions at m/z 1745.6, 1729.5, 1583.7, 1567.7, 1421.7 and 1405.7. This article highlights the discovery and identification of complex α- and ß-oligosaccharide moieties in Caulophyllum saponins by glycosidic product ions along with cross ring cleavage product ions. Five oligosaccharide moieties were unambiguously or tentatively identified as Rha → (4)Glc → (6)Glc → (4)Rha → (4)Glc → (6)Glc, Glc → (4)Glc → (6)Glc → (4)Rha → (4)Glc → (6)Glc, Rha → Glc → Glc (Glc) → (2,3)Ara, Glc → Glc (Glc) → (2,3)Ara and Glc (Glc) → (2,3)Ara. Accuracy of the analytical procedure was demonstrated by structural identification of two saponins isolated using 1D and 2D-NMR spectroscopy. The DI-ESI-IT-MS(n) method facilitates rapid discovery and analysis of trace Caulophyllum saponins and is a powerful and practical tool for structural characterization of complex oligosaccharide moieties in triterpene saponins.
[Mh] Termos MeSH primário: Caulophyllum/química
Oligossacarídeos/química
Saponinas/química
[Mh] Termos MeSH secundário: Isomerismo
Raízes de Plantas/química
Espectrometria de Massas em Tandem/métodos
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Oligosaccharides); 0 (Saponins); 0 (Triterpenes)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150605
[Lr] Data última revisão:
150605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150519
[St] Status:MEDLINE


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[PMID]:25194340
[Au] Autor:Xia YG; Li GY; Liang J; Ortori CA; Yang BY; Kuang HX; Barrett DA
[Ad] Endereço:Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK; Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, PR China.
[Ti] Título:A strategy for characterization of triterpene saponins in Caulophyllum robustum hairy roots by liquid chromatography with electrospray ionization quadrupole time-of-flight mass spectrometry.
[So] Source:J Pharm Biomed Anal;100:109-122, 2014 Nov.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Triterpene saponins are important bioactive constituents widely distributed in many plants. Saponins present in Caulophyllum (Berberidaceae) have not been fully characterized. In this study, we studied triterpene saponins from Caulophyllum robustum using liquid chromatography combined with electrospray ionization quadrupole time-of-flight mass spectrometry (LC-qTOF-MS). Rapid identification of Caulophyllum saponins was facilitated using low and high MS cone voltages to induce controlled fragmentation in positive mode. The full scan spectra at low cone voltage of 40V provided considerable structural information relating to aglycone skeletons, sugar types, and linked sequences for Caulophyllum saponins. Seven Caulophyllum aglycones were differentiated and identified by their diagnostic fragment ions combined with accurate mass measurements and characteristic fragmentation pathways. Peak intensity ratio of [aglycone+H-2H2O](+) to [aglycone+H-H2O](+) in full scan spectra acquired with low cone voltage is correlated with structural features of hederagenin and echinocystic acid and is useful for the discrimination of these positional isomers. However, at a high voltage of 200V, the saponin [M+H](+) ion and its fragmentation ions were not present; and the single saponin [M+Na](+) generated [Bα+Na](+) and [Y0α+Na](+) by in-source fragmentation, which provided structural information on the α- and ß-sugar chains in the saponins. This approach enabled simultaneous acquisition of structural information on both aglycones and sugar chains from full scan spectra in one injection. Based on the developed strategy, 51 triterpene saponins of seven different classes were fully characterized or tentatively identified, of which 32 constituents were the first to be reported in genus Caulophyllum and 18 compounds were characterized as potentially new compounds.
[Mh] Termos MeSH primário: Caulophyllum/química
Cromatografia Líquida de Alta Pressão/métodos
Raízes de Plantas/química
Saponinas/isolamento & purificação
Espectrometria de Massas por Ionização por Electrospray/métodos
Espectrometria de Massas em Tandem/métodos
Triterpenos/isolamento & purificação
[Mh] Termos MeSH secundário: Isomerismo
Estrutura Molecular
Fitoterapia
Plantas Medicinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Saponins); 0 (Triterpenes)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140908
[St] Status:MEDLINE


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[PMID]:24328138
[Au] Autor:Datta S; Mahdi F; Ali Z; Jekabsons MB; Khan IA; Nagle DG; Zhou YD
[Ad] Endereço:Department of Pharmacognosy, ‡National Center for Natural Products Research, and ⊥Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi , University, Mississippi 38677, United States.
[Ti] Título:Toxins in botanical dietary supplements: blue cohosh components disrupt cellular respiration and mitochondrial membrane potential.
[So] Source:J Nat Prod;77(1):111-7, 2014 Jan 24.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Certain botanical dietary supplements have been associated with idiosyncratic organ-specific toxicity. Similar toxicological events, caused by drug-induced mitochondrial dysfunction, have forced the withdrawal or U.S. FDA "black box" warnings of major pharmaceuticals. To assess the potential mitochondrial liability of botanical dietary supplements, extracts from 352 authenticated plant samples used in traditional Chinese, Ayurvedic, and Western herbal medicine were evaluated for the ability to disrupt cellular respiration. Blue cohosh (Caulophyllum thalictroides) methanol extract exhibited mitochondriotoxic activity. Used by some U.S. midwives to help induce labor, blue cohosh has been associated with perinatal stroke, acute myocardial infarction, congestive heart failure, multiple organ injury, and neonatal shock. The potential link between mitochondrial disruption and idiosyncratic herbal intoxication prompted further examination. The C. thalictroides methanol extract and three saponins, cauloside A (1), saponin PE (2), and cauloside C (3), exhibited concentration- and time-dependent mitochondriotoxic activities. Upon treatment, cell respiration rate rapidly increased and then dramatically decreased within minutes. Mechanistic studies revealed that C. thalictroides constituents impair mitochondrial function by disrupting membrane integrity. These studies provide a potential etiological link between this mitochondria-sensitive form of cytotoxicity and idiosyncratic organ damage.
[Mh] Termos MeSH primário: Caulophyllum/química
Respiração Celular/efeitos dos fármacos
Suplementos Nutricionais/toxicidade
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Ácido Oleanólico/análogos & derivados
Saponinas/toxicidade
[Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga
Seres Humanos
Estrutura Molecular
Ácido Oleanólico/química
Ácido Oleanólico/toxicidade
Fitoterapia
Saponinas/química
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Saponins); 17184-21-3 (cauloside A); 6SMK8R7TGJ (Oleanolic Acid); 909OGZ2782 (cauloside C)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131217
[St] Status:MEDLINE
[do] DOI:10.1021/np400758t


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[PMID]:23420136
[Au] Autor:Rader JI; Pawar RS
[Ad] Endereço:Office of Regulatory Science, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD 20740, USA. Jeanne.Rader@fda.hhs.gov
[Ti] Título:Primary constituents of blue cohosh: quantification in dietary supplements and potential for toxicity.
[So] Source:Anal Bioanal Chem;405(13):4409-17, 2013 May.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Dietary supplements containing dried roots or extracts of the roots and/or rhizomes of blue cohosh (Caulophyllum thalictroides) are widely available. This botanical has a long history of use by Native Americans and its use continues to the present day. The primary constituents of blue cohosh are its alkaloids and saponins. The structures of the alkaloids magnoflorine, baptifoline, anagyrine, and N-methylcytisine have been known for many years. The last 10 years have seen a great increase in isolation and identification of the large number of saponins present in blue cohosh. Important developments in nuclear magnetic resonance techniques have contributed substantially to the increase in elucidation of the structures of the complex saponins. Several authors have described quantitative methods for both the alkaloids and saponins in blue cohosh. Such methods have made it possible to quantify these constituents in dietary supplements containing this botanical ingredient. Concentrations of both alkaloids and saponins vary substantially in dietary supplements of blue cohosh. The nicotinic alkaloid, N-methylcytisine, a potent toxicant, has been found in all dietary supplements of blue cohosh analyzed. The teratogenic alkaloid anagyrine has been found in some but not all dietary supplements.
[Mh] Termos MeSH primário: Alcaloides/isolamento & purificação
Azocinas/isolamento & purificação
Caulophyllum/química
Suplementos Nutricionais/análise
Extratos Vegetais/análise
Saponinas/isolamento & purificação
[Mh] Termos MeSH secundário: Alcaloides/normas
Alcaloides/toxicidade
Azocinas/normas
Azocinas/toxicidade
Caulophyllum/toxicidade
Cromatografia Líquida de Alta Pressão
Cromatografia Líquida
Suplementos Nutricionais/normas
Suplementos Nutricionais/toxicidade
Feminino
Seres Humanos
Extratos Vegetais/normas
Extratos Vegetais/toxicidade
Raízes de Plantas/química
Gravidez
Quinolizinas/isolamento & purificação
Quinolizinas/normas
Quinolizinas/toxicidade
Padrões de Referência
Rizoma/química
Saponinas/normas
Saponinas/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Alkaloids); 0 (Azocines); 0 (Plant Extracts); 0 (Quinolizines); 0 (Saponins); FYU1U980Q9 (anagyrine); TT0MW69NCI (N-methylcytisine)
[Em] Mês de entrada:1310
[Cu] Atualização por classe:160512
[Lr] Data última revisão:
160512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130220
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-013-6783-7


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[PMID]:22066407
[Au] Autor:Zhang YM; Wang N; Dai BL; He LC
[Ad] Endereço:School of Medcine, Xi'an Jiaotong University, Xi'an 710061, China. zhang2008@mail.xjtu.edu.cn
[Ti] Título:[Effect of taspine derivatives on human liver cancer SMMC7721].
[So] Source:Zhong Yao Cai;34(7):1094-7, 2011 Jul.
[Is] ISSN:1001-4454
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To analyse the inhibition effect of taspine derivatives on human Liver cancer SMMC7721 cell and its mechanism. METHODS: The effects of five taspine derivatives on SMMC7721 cell growth were determined by MTT. The flow cytometry was used to determine the cell cycle. The effects of Tas-D1 on the EGF and VEGF in SMMC7721 cell were determined by ELISA. The mRNA level of EGF and VEGF in SMMC7721 cell was determined by RT-PCR. RESULTS: The MTT assay demonstrated that the taspine derivative Tas-D1 significantly inhibited the growth of SMMC7721 cell in a dose-dependent manner. Cell was stopped at S phase by Tas-D1. Tas-D1 inhibited the expression of EGF and VEGF and their mRNA in a dose-dependent manner (P<0.05). CONCLUSIONS: The taspine derivative Tas-D1 can inhibit the growth of human Liver cancer SMMC7721 cell and change cell cycle, which may be related to the inhibition of EGF and VEGF expression.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Antineoplásicos/farmacologia
Proliferação Celular/efeitos dos fármacos
Fator de Crescimento Epidérmico/metabolismo
Neoplasias Hepáticas/patologia
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Mh] Termos MeSH secundário: Alcaloides/administração & dosagem
Antineoplásicos/administração & dosagem
Carcinoma Hepatocelular/metabolismo
Carcinoma Hepatocelular/patologia
Caulophyllum/química
Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Fator de Crescimento Epidérmico/genética
Citometria de Fluxo
Seres Humanos
Neoplasias Hepáticas/metabolismo
Reação em Cadeia da Polimerase
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Antineoplastic Agents); 0 (RNA, Messenger); 0 (Vascular Endothelial Growth Factor A); 62229-50-9 (Epidermal Growth Factor); V53XN9L07O (taspine)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111110
[St] Status:MEDLINE


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[PMID]:21872415
[Au] Autor:Avula B; Wang YH; Rumalla CS; Ali Z; Smillie TJ; Khan IA
[Ad] Endereço:National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, MS 38677, USA. bavula@olemiss.edu
[Ti] Título:Analytical methods for determination of magnoflorine and saponins from roots of Caulophyllum thalictroides (L.) Michx. using UPLC, HPLC and HPTLC.
[So] Source:J Pharm Biomed Anal;56(5):895-903, 2011 Dec 15.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Analytical methods including HPLC, UPLC and HPTLC are presented for the determination of major alkaloid and triterpene saponins from the roots of Caulophyllum thalictroides (L.) Michx. (blue cohosh) and dietary supplements claiming to contain blue cohosh. A separation by LC was achieved using a reversed phase column, PDA with ELS detection, and ammonium acetate/acetonitrile gradient as the mobile phase. Owing to their low UV absorption, the triterpene saponins were detected by evaporative light scattering. The eight triterpene saponins (cauloside H, leonticin D, cauloside G, cauloside D, cauloside B, cauloside C, cauloside A and saponin PE) and the alkaloid magnoflorine could be separated within 35 min using HPLC method and within 8.0 min using UPLC method with detection limits of 10 µg/mL for saponins and 1 µg/mL for magnoflorine. The detection wavelength was 320 nm for magnoflorine and ELS detection was used for the eight saponins. The methods were also successfully applied to analyze different dietary supplements. For the products claiming to contain blue cohosh, there was a significant variability in the amounts of triterpene saponins detected. Calculations based on the analysis results for dietary supplements showed that maximum daily intake of alkaloid and saponins vary with the form (solids/liquids) and recommended doses according to the products label. Intakes varied from 0.57 to 15.8 mg/day for magnoflorine and from 5.97 to 302.4 mg/day for total saponins. LC-mass spectrometry coupled with electrospray ionization (ESI) method is described for the identification and confirmation of nine compounds in plant samples and dietary products. A HPTLC method was also developed for the fast chemical fingerprint analysis of C. thalictroides samples.
[Mh] Termos MeSH primário: Aporfinas/análise
Caulophyllum/química
Cromatografia Líquida/métodos
Raízes de Plantas/química
Saponinas/análise
[Mh] Termos MeSH secundário: Aporfinas/química
Sequência de Carboidratos
Limite de Detecção
Dados de Sequência Molecular
Reprodutibilidade dos Testes
Saponinas/química
Espectrofotometria Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Aporphines); 0 (Saponins); NI8K6962K4 (magnoflorine)
[Em] Mês de entrada:1201
[Cu] Atualização por classe:121115
[Lr] Data última revisão:
121115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110830
[St] Status:MEDLINE
[do] DOI:10.1016/j.jpba.2011.07.028


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[PMID]:21596111
[Au] Autor:Wang XL; Liu BR; Chen CK; Wang JR; Lee SS
[Ad] Endereço:Key Laboratory of Phytochemistry of Shaanxi Province, Baoji University of Arts and Sciences, Baoji 721013, China. xlwangwang@163.com
[Ti] Título:Four new fluorenone alkaloids and one new dihydroazafluoranthene alkaloid from Caulophyllum robustum Maxim.
[So] Source:Fitoterapia;82(6):793-7, 2011 Sep.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Four new fluorenone alkaloids, caulophylline A-D (1-4), and one new dihydroazafluoranthene alkaloid, caulophylline E (5) were isolated from the roots of Caulophyllum robustum Maxim. Their structures were elucidated by spectroscopic analysis. Among the isolated alkaloids, Caulophylline E showed good scavenging effects against DPPH radical with IC(50) of 39 µM.
[Mh] Termos MeSH primário: Alcaloides/isolamento & purificação
Caulophyllum/química
Fluorenos/química
Fluorenos/isolamento & purificação
[Mh] Termos MeSH secundário: Alcaloides/química
Alcaloides/farmacologia
Compostos de Bifenilo/antagonistas & inibidores
Dibenzazepinas/química
Dibenzazepinas/isolamento & purificação
Dibenzazepinas/farmacologia
Fluorenos/farmacologia
Concentração Inibidora 50
Medicina Tradicional Chinesa
Estrutura Molecular
Picratos/antagonistas & inibidores
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Biphenyl Compounds); 0 (Dibenzazepines); 0 (Fluorenes); 0 (Picrates); 58335-95-8 (dihydroazapetine); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110521
[St] Status:MEDLINE
[do] DOI:10.1016/j.fitote.2011.04.010


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[PMID]:21259434
[Au] Autor:Hou X; Wang S; Hou J; He L
[Ad] Endereço:School of Medicine, Xi'an Jiaotong University, Shaanxi, PR China.
[Ti] Título:Establishment of A431 cell membrane chromatography-RPLC method for screening target components from Radix Caulophylli.
[So] Source:J Sep Sci;34(5):508-13, 2011 Mar.
[Is] ISSN:1615-9314
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We describe here an analytical method of A431 cell membrane chromatography (A431/CMC) (CMC, cell membrane chromatography) combined with RPLC for recognition, separation, and identification of target components from traditional Chinese medicines (TCMs) Radix Caulophylli. The A431 cells with high expressed epidermal growth factor receptor (EGFR) were used to prepare the stationary phase in the CMC model. Retention fractions on the A431-CMC model were collected using an automated fraction collection and injection module (FC/I). Each fraction was analyzed by RPLC under the optimized conditions. Gefitinib and erlotinib were used as standard compounds to investigate the suitability and reliability of the A431 cell membrane chromatography-RPLC method prior to screening target component from Radix Caulophylli total alkaloids. The results indicated that caulophine and taspine were the target component acting on the epidermal growth factor receptor. This method could be an efficient way in drug discovery using natural medicinal herbs as a source of novel compounds.
[Mh] Termos MeSH primário: Caulophyllum/química
Cromatografia Líquida de Alta Pressão/métodos
Medicamentos de Ervas Chinesas/química
[Mh] Termos MeSH secundário: Alcaloides/química
Linhagem Celular
Membrana Celular/química
Cromatografia Líquida de Alta Pressão/instrumentação
Cromatografia de Fase Reversa/instrumentação
Cromatografia de Fase Reversa/métodos
Seres Humanos
Ligação Proteica
Receptor do Fator de Crescimento Epidérmico/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Drugs, Chinese Herbal); EC 2.7.10.1 (Receptor, Epidermal Growth Factor)
[Em] Mês de entrada:1105
[Cu] Atualização por classe:110223
[Lr] Data última revisão:
110223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110125
[St] Status:MEDLINE
[do] DOI:10.1002/jssc.201000643


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[PMID]:20829129
[Au] Autor:Sun M; Ren J; Du H; Zhang Y; Zhang J; Wang S; He L
[Ad] Endereço:Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, China.
[Ti] Título:A combined A431 cell membrane chromatography and online high performance liquid chromatography/mass spectrometry method for screening compounds from total alkaloid of Radix Caulophylli acting on the human EGFR.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;878(28):2712-8, 2010 Oct 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We have developed an online analytical method that combines A431 cell membrane chromatography (A431/CMC) with high performance liquid chromatography and mass spectrometry (LC/MS) for identifying active components from Radix Caulophylli acting on human EGFR. Retention fractions on A431/CMC model were captured onto an enrichment column and the components were directly analyzed by combining a 10-port column switcher with an LC/MS system for separation and preliminary identification. Using Sorafenib tosylate as a positive control, taspine and caulophine from Radix Caulophylli were identified as the active molecules which could act on the EGFR. This A431/CMC-online-LC/MS method can be applied for screening active components acting on EGFR from traditional Chinese medicines exemplified by Radix Caulophylli and will be of great utility in drug discovery using natural medicinal herbs as a source of novel compounds.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Caulophyllum/química
Cromatografia Líquida de Alta Pressão/métodos
Avaliação Pré-Clínica de Medicamentos/métodos
Espectrometria de Massas/métodos
Receptor do Fator de Crescimento Epidérmico/metabolismo
[Mh] Termos MeSH secundário: Alcaloides/química
Alcaloides/metabolismo
Linhagem Celular Tumoral
Membrana Celular/química
Membrana Celular/metabolismo
Cromatografia Líquida de Alta Pressão/instrumentação
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/metabolismo
Medicamentos de Ervas Chinesas/farmacologia
Fluorenos/química
Fluorenos/metabolismo
Fluorenos/farmacologia
Seres Humanos
Ligações de Hidrogênio
Raízes de Plantas/química
Ligação Proteica
Receptor do Fator de Crescimento Epidérmico/agonistas
Receptor do Fator de Crescimento Epidérmico/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Drugs, Chinese Herbal); 0 (Fluorenes); 0 (caulophine); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); V53XN9L07O (taspine)
[Em] Mês de entrada:1101
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100911
[St] Status:MEDLINE
[do] DOI:10.1016/j.jchromb.2010.08.010


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[PMID]:20707411
[Au] Autor:Wu M; Hu Y; Ali Z; Khan IA; Verlangeiri AJ; Dasmahapatra AK
[Ad] Endereço:National Center for Natural Product Research, School of Pharmacy, University of Mississippi, University, MS, USA.
[Ti] Título:Teratogenic effects of blue cohosh (Caulophyllum thalictroides) in Japanese medaka (Oryzias latipes) are probably mediated through GATA2/EDN1 signaling pathway.
[So] Source:Chem Res Toxicol;23(8):1405-16, 2010 Aug 16.
[Is] ISSN:1520-5010
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Blue cohosh (Caulophyllum thalictroides) (BC) has been used widely to induce labor and to treat other uterine conditions. However, the safety and effectiveness of this herbal product has not yet been evaluated by the US Food and Drug Administration (FDA). Several conflicting reports indicated that the root extract of BC is a teratogen and, by some unknown mechanisms, is able to induce cardiovascular malfunctions in new-born babies. To understand the mechanism, we have used Japanese medaka (Oryzias latipes) embryo-larval development as the experimental model and the methanolic extract of BC root as the teratogen. The embryo mortality, hatching efficiency, and morphological abnormalities in craniofacial and cardiovascular systems are considered for the evaluation of BC toxicity. Our results indicate that BC is able to disrupt cardiovascular and craniofacial cartilage development in medaka embryo in a dose and developmental stage-specific manner. Moreover, embryos in precirculation are to some extent more resistant to BC than ones with circulation. By using subtractive hybridization, we have observed that gata2 mRNA was differentially expressed in the circulating embryos after BC treatment. As GATA-binding sequences are required for the expression of the endothelin1 (edn1) gene and edn1 expressed in blood vessels and craniofacial cartilages, we have extended our investigations to edn1 gene expression regulation by BC. We found that edn1, furin1, and endothelin receptor A (ednrA) genes are developmentally regulated; endothelin converting enzyme mRNA (ece1) maintained a steady-state level throughout development. Circulating medaka embryos (3 days post fertilization, dpf) exposed to BC (10 microg/mL) for 48 h have increased levels of gata2, ece1, and preproenodthelin (preproedn1) mRNA contents; however, other mRNAs (furin and ednrA) remained unaltered. Therefore, the enhanced expression of gata2 mRNA followed by ece1 and preproedn1 mRNA by BC might be able to induce vasoconstriction and cardiovascular defects and disrupt craniofacial cartilages in medaka embryos. We conclude that cardiovascular and craniofacial defects in medaka embryogenesis by BC are probably mediated through a GATA2-EDN1 signaling pathway.
[Mh] Termos MeSH primário: Caulophyllum/química
Caulophyllum/toxicidade
Endotelina-1/metabolismo
Fator de Transcrição GATA2/metabolismo
Oryzias/embriologia
Oryzias/metabolismo
Transdução de Sinais/efeitos dos fármacos
Teratogênios/toxicidade
[Mh] Termos MeSH secundário: Animais
Ácido Aspártico Endopeptidases/genética
Ácido Aspártico Endopeptidases/metabolismo
Relação Dose-Resposta a Droga
Endotelina-1/genética
Enzimas Conversoras de Endotelina
Fator de Transcrição GATA2/genética
Metaloendopeptidases/genética
Metaloendopeptidases/metabolismo
Raízes de Plantas/química
Raízes de Plantas/toxicidade
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Transdução de Sinais/genética
Teratogênios/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Endothelin-1); 0 (GATA2 Transcription Factor); 0 (RNA, Messenger); 0 (Teratogens); EC 3.4.23.- (Aspartic Acid Endopeptidases); EC 3.4.24.- (Metalloendopeptidases); EC 3.4.24.71 (Endothelin-Converting Enzymes)
[Em] Mês de entrada:1102
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100817
[St] Status:MEDLINE
[do] DOI:10.1021/tx100205a



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