Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.125.500.500 [Categoria DeCS]
Referências encontradas : 18 [refinar]
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  1 / 18 MEDLINE  
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[PMID]:26359402
[Au] Autor:Lau W; Sattely ES
[Ad] Endereço:Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
[Ti] Título:Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
[So] Source:Science;349(6253):1224-8, 2015 Sep 11.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Podophyllotoxin is the natural product precursor of the chemotherapeutic etoposide, yet only part of its biosynthetic pathway is known. We used transcriptome mining in Podophyllum hexandrum (mayapple) to identify biosynthetic genes in the podophyllotoxin pathway. We selected 29 candidate genes to combinatorially express in Nicotiana benthamiana (tobacco) and identified six pathway enzymes, including an oxoglutarate-dependent dioxygenase that closes the core cyclohexane ring of the aryltetralin scaffold. By coexpressing 10 genes in tobacco-these 6 plus 4 previously discovered-we reconstitute the pathway to (-)-4'-desmethylepipodophyllotoxin (the etoposide aglycone), a naturally occurring lignan that is the immediate precursor of etoposide and, unlike podophyllotoxin, a potent topoisomerase inhibitor. Our results enable production of the etoposide aglycone in tobacco and circumvent the need for cultivation of mayapple and semisynthetic epimerization and demethylation of podophyllotoxin.
[Mh] Termos MeSH primário: Etoposídeo/metabolismo
Engenharia Genética
Oxigenases de Função Mista/metabolismo
Podofilotoxina/análogos & derivados
Podofilotoxina/metabolismo
Podophyllum peltatum/enzimologia
Tabaco/metabolismo
Inibidores da Topoisomerase/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Vias Biossintéticas/genética
Regulação Enzimológica da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Metilação
Oxigenases de Função Mista/genética
Dados de Sequência Molecular
Podofilotoxina/biossíntese
Podophyllum peltatum/genética
Tabaco/genética
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Topoisomerase Inhibitors); 6PLQ3CP4P3 (Etoposide); EC 1.- (Mixed Function Oxygenases); L36H50F353 (Podophyllotoxin)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:161025
[Lr] Data última revisão:
161025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150912
[St] Status:MEDLINE
[do] DOI:10.1126/science.aac7202


  2 / 18 MEDLINE  
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[PMID]:26359390
[Au] Autor:O'Connor SE
[Ad] Endereço:John Innes Centre, Department of Biological Chemistry, Norwich NR4 7UK, UK. sarah.o'connor@jic.ac.uk.
[Ti] Título:PLANT BIOCHEMISTRY. Fighting cancer while saving the mayapple.
[So] Source:Science;349(6253):1167-8, 2015 Sep 11.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Etoposídeo/metabolismo
Engenharia Genética
Oxigenases de Função Mista/metabolismo
Podofilotoxina/análogos & derivados
Podofilotoxina/metabolismo
Podophyllum peltatum/enzimologia
Tabaco/metabolismo
Inibidores da Topoisomerase/metabolismo
[Pt] Tipo de publicação:COMMENT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Topoisomerase Inhibitors); 6PLQ3CP4P3 (Etoposide); EC 1.- (Mixed Function Oxygenases); L36H50F353 (Podophyllotoxin)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150911
[Lr] Data última revisão:
150911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150912
[St] Status:MEDLINE
[do] DOI:10.1126/science.aad1801


  3 / 18 MEDLINE  
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[PMID]:26289300
[Au] Autor:Arneaud SL; Porter JR
[Ad] Endereço:Department of Biological Sciences, University of the Sciences, 600 South 43rd Street, Philadelphia, PA, 19104, USA. sarneaud@mail.usciences.edu.
[Ti] Título:Investigation and Expression of the Secoisolariciresinol Dehydrogenase Gene Involved in Podophyllotoxin Biosynthesis.
[So] Source:Mol Biotechnol;57(11-12):961-73, 2015 Dec.
[Is] ISSN:1559-0305
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic fungus Phialocephala podophylli (strain PPE7) that we isolated from the rhizomes of Podophyllum peltatum and is known to produce detectable amounts of PPT in broth culture. To date, the complete PPT biosynthetic pathway has yet to be determined in any species. Since fungi are well known for clustering genes that belong to secondary metabolite pathways, use of a fungal system for investigation of the PPT biosynthesis genes may ultimately lead to elucidation of the entire pathway. In this study, we investigated the secoisolariciresinol dehydrogenase (SD) gene that facilitates the dehydrogenation of secoisolariciresinol to form matairesinol, a mid-pathway intermediate product in PPT biosynthesis. We utilized PCR amplification to acquire the complete SD gene sequence in PPE7 and opted to synthesize the P. peltatum SD sequence for expression. Through western blotting, we confirmed the expression of the recombinant SD (PpSD) and verified protein functionality with a bioconversion assay followed by HPLC and LC-MS analyses. Here, we report the identification of the SD gene in PPE7; this is the first report of the SD gene in an endophytic fungus. Additionally, we established the groundwork for the future expression of the complete PPT biosynthetic pathway in the heterologous host Pichia pastoris.
[Mh] Termos MeSH primário: Ascomicetos/enzimologia
Proteínas Fúngicas/metabolismo
Álcool Oxidorredutases Dependentes de NAD(+) e NADP(+)/metabolismo
Podofilotoxina/biossíntese
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Ascomicetos/genética
Ascomicetos/isolamento & purificação
Sequência de Bases
Vias Biossintéticas
Clonagem Molecular
Meios de Cultura
DNA Fúngico/genética
Proteínas Fúngicas/genética
Dados de Sequência Molecular
Álcool Oxidorredutases Dependentes de NAD(+) e NADP(+)/genética
Podophyllum peltatum/microbiologia
Conformação Proteica
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); 0 (DNA, Fungal); 0 (Fungal Proteins); EC 1.1.1.- (NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases); EC 1.1.1.331 (secoisolariciresinol dehydrogenase); L36H50F353 (Podophyllotoxin)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150821
[St] Status:MEDLINE
[do] DOI:10.1007/s12033-015-9888-8


  4 / 18 MEDLINE  
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[PMID]:25282651
[Au] Autor:Wang H; Tang L; Tang Y; Yuan Z
[Ad] Endereço:School of Public Health, Wuhan University, 185 Donghu Road, Wuhan, Hubei 430071, PR China.
[Ti] Título:SAR analysis and biological studies of synthesized podophyllum derivates obtained by N linkage modification at C-4 position.
[So] Source:Bioorg Med Chem;22(21):6183-92, 2014 Nov 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of C4-N-substituted podophyllum derivatives were synthesized and tested for cytotoxicity in HeLa, BGC-823, A549, Huh7 and MCF-7 cells by MTT assay. Pharmacologically, most derivatives displayed potent cytotoxicity against at least one of the tested tumor cell lines. Structure activity relationship (SAR) analysis suggests that compounds with imidogen exposed on the pyridine, rather than pyrimidine, exhibited significantly elevated potency. Moreover, the presence of a chlorine atom in the heterocyclic ring enhanced cytotoxicity, with the order 3-position>4-position>5-position>6-position. Specifically, two compounds, 3g and 3h, with 2-amino-3-chloropyridine substituted into the podophyllotoxin (PPT) and 4'-O-demethylepipodophyllotoxin (DMEP) scaffolds were shown to have the most potent HeLa cells cytotoxicity compared to other synthesized derivatives or reference compounds PPT, DMEP and etoposide (VP-16). The compound 3g was shown to inhibit microtubule polymerization and compound 3h affected topoisomerase II catalytic activity. Both compounds resulted in G2/M phase arrest and apoptosis, purportedly by increasing the expression of P53, followed by Bax up-regulation, Bcl-2 down-regulation, and caspase-3 activation. As a result of this work, we conclude that compounds 3g and 3h are more potent anticancer agents than VP-16, and that they work by different antitumor mechanisms.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Podofilotoxina/química
Podofilotoxina/farmacologia
Podophyllum peltatum/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/síntese química
Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos
Seres Humanos
Pontos de Checagem da Fase M do Ciclo Celular/efeitos dos fármacos
Neoplasias/tratamento farmacológico
Podofilotoxina/síntese química
Relação Estrutura-Atividade
Moduladores de Tubulina/síntese química
Moduladores de Tubulina/química
Moduladores de Tubulina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Tubulin Modulators); L36H50F353 (Podophyllotoxin)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:141202
[Lr] Data última revisão:
141202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141006
[St] Status:MEDLINE


  5 / 18 MEDLINE  
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[PMID]:22495446
[Au] Autor:Minnis AM; McTaggart AR; Rossman AY; Aime MC
[Ad] Endereço:Systematic Mycology & Microbiology Laboratory, USDA-ARS, B010A, Beltsville, MD, USA. maime@agcenter.lsu.edu
[Ti] Título:Taxonomy of mayapple rust: the genus Allodus resurrected.
[So] Source:Mycologia;104(4):942-50, 2012 Jul-Aug.
[Is] ISSN:0027-5514
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mayapple rust is a common, disfiguring disease that is widespread in temperate eastern North America wherever the host, Podophyllum peltatum, occurs. Puccinia podophylli, the etiological agent of this rust, has been shown to be distantly related to both Puccinia and Uromyces as exemplified by their types. A systematic study was made to determine the generic classification of P. podophylli. Phylogenetic analyses of two rDNA loci from multiple specimens support the recognition of this taxon as a separate genus of Pucciniaceae. Based on historical literature and type material, P. podophylli was found to represent the type of the forgotten genus Allodus and it is correctly named Allodus podophylli. A neotype is designated for Puccinia podophylli Schwein. (≡ Allodus podophylli) and a lectotype is designated for Aecidium podophylli.
[Mh] Termos MeSH primário: Basidiomycota/classificação
DNA Fúngico/genética
Podophyllum peltatum/microbiologia
[Mh] Termos MeSH secundário: Basidiomycota/citologia
Basidiomycota/genética
Basidiomycota/patogenicidade
DNA Ribossômico/genética
Loci Gênicos
Técnicas de Tipagem Micológica
Filogenia
Doenças das Plantas/microbiologia
Folhas de Planta/microbiologia
Alinhamento de Sequência
Análise de Sequência de DNA
Esporos Fúngicos/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1209
[Cu] Atualização por classe:120801
[Lr] Data última revisão:
120801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120413
[St] Status:MEDLINE
[do] DOI:10.3852/11-350


  6 / 18 MEDLINE  
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[PMID]:21664168
[Au] Autor:Zhao S; Baik OD
[Ad] Endereço:Department of Chemical and Biological Engineering, College of Engineering, University of Saskatchewan, 57 Campus Dr., Saskatoon, SK, Canada S7N 5A9.
[Ti] Título:Application of ultrasound as pretreatment for extraction of podophyllotoxin from rhizomes of Podophyllum peltatum.
[So] Source:Ultrason Sonochem;19(1):22-31, 2012 Jan.
[Is] ISSN:1873-2828
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The effect of high-power ultrasound pretreatment on the extraction of podophyllotoxin from Podophyllum peltatum was investigated. Direct sonication by an ultrasound probe horn was applied at 24 kHz and a number of factors were investigated: particle size (0.18-0.6 mm), type of solvent (0-100% aqueous ethanol), ultrasonic treatment time (2-40 min), and power of ultrasound (0-100% power intensity, maximum power: 78 W). The optimal condition of ultrasound was achieved with 0.425-0.6 mm particle size, 10 min sonication time, 35 W ultrasound power, and water as the medium. There was no obvious degradation of podophyllotoxin with ultrasound under the applied conditions, and an improvement in extractability was observed. The SEM microscopic structure change of treated samples disclosed the effect of ultrasound on the tissue cells. The increased pore volume and surface area after ultrasonic treatment also confirmed the positive effect of ultrasound pretreatment on the extraction yield of podophyllotoxin from the plant cells.
[Mh] Termos MeSH primário: Podofilotoxina/isolamento & purificação
Podophyllum peltatum/química
Rizoma/química
Ultrassom
[Mh] Termos MeSH secundário: Tamanho da Partícula
Propriedades de Superfície
Ultrassom/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
L36H50F353 (Podophyllotoxin)
[Em] Mês de entrada:1112
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110614
[St] Status:MEDLINE
[do] DOI:10.1016/j.ultsonch.2011.05.012


  7 / 18 MEDLINE  
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[PMID]:21374649
[Au] Autor:Avula B; Wang YH; Moraes RM; Khan IA
[Ad] Endereço:National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA.
[Ti] Título:Rapid analysis of lignans from leaves of Podophyllum peltatum L. samples using UPLC-UV-MS.
[So] Source:Biomed Chromatogr;25(11):1230-6, 2011 Nov.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new rapid UPLC-UV-MS method has been developed that permits the analysis of four lignans (4'-O-demethylpodophyllotoxin, podophyllotoxin, α-peltatin and ß-peltatin) in P. peltatum L. Podophyllotoxin is a natural lignan that is being used as a precursor for the semi-synthetic anti-cancer drugs etoposide, teniposide and etopophos. The chromatographic separation was achieved using a reversed-phase C18 column with a mobile phase of water and acetonitrile, both containing 0.05% formic acid. Analyses of P. peltatum leaves collected from different colonies within a single site indicated a significant variation in 4'-O-demethylpodophyllotoxin, α-peltatin, podophyllotoxin and ß-peltatin content. Within 3.0 min four main lignans could be separated with detection limits of 0.1, 0.3, 0.3 and 0.2 µg/mL, respectively. 4'-O-demethylpodophyllotoxin and α-peltatin appeared most prominently among the lignans obtained. The podophyllotoxin content was found in the range of 0.004-0.77% from 16 samples collected from 6 colonies within the same site. The content of podophyllotoxin is directly proportional to the content of 4'-O-demethylpodophyllotoxin and inversely proportional to α-peltatin and ß-peltatin content. LC-mass spectrometry coupled with electrospray ionization (ESI) interface method is described for the identification of four lignans in various populations of plant samples. By applying principal component analysis and hierarchical cluster analysis, Podophyllum samples collected from various colonies within a location were distinguished.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Folhas de Planta/química
Podofilotoxina/análogos & derivados
Podofilotoxina/análise
Podophyllum peltatum/química
[Mh] Termos MeSH secundário: Análise por Conglomerados
Limite de Detecção
Modelos Lineares
Espectrometria de Massas/métodos
Extratos Vegetais/química
Podofilotoxina/química
Análise de Componente Principal
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Plant Extracts); L36H50F353 (Podophyllotoxin)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:140807
[Lr] Data última revisão:
140807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110305
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.1595


  8 / 18 MEDLINE  
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[PMID]:19325943
[Au] Autor:Ray LE
[Ad] Endereço:Cleveland Marshall College of Law, Cleveland State University, OH, USA. laura.ray@law.csuohio.edu
[Ti] Título:Podophyllum peltatum and observations on the Creek and Cherokee Indians: William Bartram's preservation of Native American pharmacology.
[So] Source:Yale J Biol Med;82(1):25-36, 2009 Mar.
[Is] ISSN:1551-4056
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Historians have examined the significant contributions John and William Bartram made to 18th- and 19th-century knowledge of indigenous North American flora. However, the Bartrams' contribution to medicinal botanical knowledge, particularly William Bartram's compilation of Indians' knowledge on the preparation and use of medicinal botanicals, is not well-known. In addition, while William Bartram's contemporaries relied on his accounts of medicinal botanicals, they rarely acknowledged Bartram or Indians in their own works. Contemporaries plagiarized Bartram's writings and used his exquisite illustrations to ornament their own publications. This paper reconstructs William Bartram's careful collection and recording of medicinal botanical knowledge that became part of late 18th- and early 19th-century American pharmacology, as well as provides evidence for 54 Bartram-identified indigenous species and the pirating of William Bartram's work by contemporaries.
[Mh] Termos MeSH primário: Etnofarmacologia/história
Índios Norte-Americanos/história
Fitoterapia/história
Podophyllum peltatum
[Mh] Termos MeSH secundário: História do Século XVIII
História do Século XIX
Seres Humanos
Estados Unidos
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE
[Ps] Nome de pessoa como assunto:Bartram W
[Em] Mês de entrada:0905
[Cu] Atualização por classe:141210
[Lr] Data última revisão:
141210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090328
[St] Status:MEDLINE


  9 / 18 MEDLINE  
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[PMID]:17852156
[Au] Autor:Chou SL; Chou MY; Kao WF; Yen DH; Huang CI; Lee CH
[Ad] Endereço:Department of Emergency Medicine, Taipei Veterans Hospital, Taipei, Taiwan, Republic of China.
[Ti] Título:Cessation of nail growth following Bajiaolian intoxication.
[So] Source:Clin Toxicol (Phila);46(2):159-63, 2008 Feb.
[Is] ISSN:1556-3650
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bajiaolian (Dysosma pleianthum), a species in the Mayapple family (Podophyllum pelatum), has been widely used as a traditional Chinese herbal medication for the remedies of snake bite, tumor growth, post-partum recovery, and acne. It has also been used in western medicine, especially topically for various skin lesions. Both oral ingestion and dermal application may result in severe toxicity. The clinical presentations reported after Bajiaolian poisoning include nausea, vomiting, diarrhea, abdominal cramps, tachycardia, orthostatic hypotension, paralytic ileus, urinary retention, hepatorenal dysfunction, leukocytosis followed by leukopenia, thrombocytopenia, prolonged areflexia, prolonged paraethesia and sensory ataxia, dizziness, fever, memory impairment, hallucinations, paranoia, convulsion, fainting, and coma. There are no previous reports in the literature about the cessation of nail growth as a clinical presentation following Bajiaolian poisoning. We present a case of nail growth that was halted for more than seven years after a single case of Bajiaolian poisoning.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/envenenamento
Unhas/efeitos dos fármacos
Podophyllum peltatum/química
[Mh] Termos MeSH secundário: Adulto
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/uso terapêutico
Feminino
Cefaleia/tratamento farmacológico
Seres Humanos
Doenças da Unha/induzido quimicamente
Doenças da Unha/patologia
Unhas/crescimento & desenvolvimento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:0805
[Cu] Atualização por classe:091117
[Lr] Data última revisão:
091117
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:070914
[St] Status:MEDLINE


  10 / 18 MEDLINE  
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[PMID]:16933860
[Au] Autor:Eyberger AL; Dondapati R; Porter JR
[Ad] Endereço:Cell Biology & Biotechnology Program, Department of Biological Sciences, University of the Sciences in Philadelphia, 600 S. 43rd Street, Philadelphia, Pennsylvania 19104, USA.
[Ti] Título:Endophyte fungal isolates from Podophyllum peltatum produce podophyllotoxin.
[So] Source:J Nat Prod;69(8):1121-4, 2006 Aug.
[Is] ISSN:0163-3864
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The lignan podophyllotoxin (1) is highly valued as the precursor to clinically useful anticancer drugs. Substantial drug development of this compound class continues, including potential new use for inflammatory disease. We have isolated two endophyte fungi, both strains of Phialocephala fortinii, from rhizomes of the plant Podophyllum peltatum. The fungi were identified through DNA sequencing and morphology. Both strains of fungi are slow-growing and produce 1 at low but measurable amounts in broth culture. The compound was confirmed through matching HPLC retention times, absorption spectra, and MS data to authentic 1. The yield of 1 has ranged from 0.5 to 189 microg/L in 4 weeks of culture. These fungi have implications for the sustained production of 1 independent of wild populations of the source plants.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Ascomicetos/química
Podofilotoxina/isolamento & purificação
Podophyllum peltatum/química
Pró-Fármacos/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Artemia/efeitos dos fármacos
Ascomicetos/metabolismo
DNA Fúngico/química
DNA Fúngico/genética
DNA Fúngico/isolamento & purificação
Estrutura Molecular
Podofilotoxina/química
Podofilotoxina/farmacologia
Pró-Fármacos/química
Pró-Fármacos/farmacologia
Rizoma/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (DNA, Fungal); 0 (Prodrugs); L36H50F353 (Podophyllotoxin)
[Em] Mês de entrada:0609
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060829
[St] Status:MEDLINE



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