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Pesquisa : B01.650.940.800.575.912.250.157 [Categoria DeCS]
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  1 / 1756 MEDLINE  
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[PMID]:28741076
[Au] Autor:Tzelepis G; Bejai S; Sattar MN; Schwelm A; Ilbäck J; Fogelqvist J; Dixelius C
[Ad] Endereço:Department of Plant Biology, Uppsala BioCenter, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, P.O. Box 7080, 75007, Uppsala, Sweden. Georgios.Tzelepis@slu.se.
[Ti] Título:Detection of Verticillium species in Swedish soils using real-time PCR.
[So] Source:Arch Microbiol;199(10):1383-1389, 2017 Dec.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Verticillium species are soilborne plant pathogens, responsible for big yield losses worldwide. Here, we report improved procedures to generate DNA from Verticillium species imbedded in farm soils. Using new genomic sequence information, primers for V. dahliae, V. albo-atrum, V. tricorpus, and V. longisporum were designed. In a survey of 429 samples from intensively farmed soil of two Swedish regions, only V. dahliae and V. longisporum were identified. A bias towards V. longisporum (40%) was seen in the south, whereas V. dahliae was more frequent in the western region (19%). Analyses of soil and leaf samples from 20 sugar beet fields, where foliar wilting had been observed, revealed V. dahliae DNA in all leaf and soil samples and V. longisporum in 18 soil samples, illustrating host choice and longevity of the V. longisporum microsclerotia. This study demonstrates the applicability of new molecular diagnostic tools that are important for growers of variable crops.
[Mh] Termos MeSH primário: Brassicaceae/microbiologia
DNA Fúngico/genética
Verticillium/genética
Verticillium/isolamento & purificação
[Mh] Termos MeSH secundário: Primers do DNA/genética
Doenças das Plantas/microbiologia
Reação em Cadeia da Polimerase em Tempo Real
Solo/química
Microbiologia do Solo
Suécia
Verticillium/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Fungal); 0 (Soil)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-017-1412-z


  2 / 1756 MEDLINE  
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[PMID]:29329302
[Au] Autor:Wang MN; Duan L; Qiao Q; Wang ZF; Zimmer EA; Li ZC; Chen HF
[Ad] Endereço:Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
[Ti] Título:Phylogeography and conservation genetics of the rare and relict Bretschneidera sinensis (Akaniaceae).
[So] Source:PLoS One;13(1):e0189034, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bretschneidera sinensis, a class-I protected wild plant in China, is a relic of the ancient Tertiary tropical flora endemic to Asia. However, little is known about its genetics and phylogeography. To elucidate the current phylogeographic patterns and infer the historical population dynamics of B. sinensis, and to make recommendations for its conservation, three non-coding regions of chloroplast DNA (trnQ-rps16, rps8-rps11, and trnT-trnL) were amplified and sequenced across 256 individuals from 23 populations of B. sinensis, spanning 10 provinces of China. We recognized 13 haplotypes, demonstrating relatively high total haplotype diversity (hT = 0.739). Almost all of the variation existed among populations (98.09%, P < 0.001), but that within populations was low (1.91%, P < 0.001). Strong genetic differentiation was detected among populations (GST = 0.855, P < 0.001) with limited estimations of seed flow (Nm = 0.09), indicating that populations were strongly isolated from one another. According to SAMOVA analysis, populations of B. sinensis in China could be divided into five geographic groups: (1) eastern Yunnan to western Guangxi; (2) Guizhou-Hunan-Hubei; (3) central Guangdong; (4) northwestern Guangdong; and (5) the Luoxiao-Nanling-Wuyi -Yangming Mountain. Network analysis showed that the most ancestral haplotypes were located in the first group, i.e., the eastern Yungui Plateau and in eastern Yunnan, which is regarded as a putative glacial refugia for B. sinensis in China. B. sinensis may have expanded its range eastward from these refugia and experienced bottleneck or founder effects in southeastern China. Populations in Liping (Guizhou Province), Longsheng (Guangxi Province), Huizhou (Guangdong Province), Chongyi (Jiangxi Province), Dong-an (Hunan Province), Pingbian (Yunnan Province) and Xinning (Hunan Province) are proposed as the priority protection units.
[Mh] Termos MeSH primário: Brassicaceae/classificação
Brassicaceae/genética
Conservação dos Recursos Naturais
Genes de Plantas
Filogeografia
[Mh] Termos MeSH secundário: China
DNA de Cloroplastos/genética
Variação Genética
Haplótipos
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Chloroplast)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189034


  3 / 1756 MEDLINE  
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[PMID]:27771868
[Au] Autor:Schmidt MA; Pendarvis K
[Ad] Endereço:School of Plant Sciences, BIO5 Institute, University of Arizona, Tucson, AZ, USA. monicaschmidt@email.arizona.edu.
[Ti] Título:Proteome rebalancing in transgenic Camelina occurs within the enlarged proteome induced by ß-carotene accumulation and storage protein suppression.
[So] Source:Transgenic Res;26(2):171-186, 2017 04.
[Is] ISSN:1573-9368
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Oilseed crops are global commodities for their oil and protein seed content. We have engineered the oilseed Camelina sativa to exhibit increased protein content with a slight decrease in oil content. The introduction of a phytoene synthase gene with an RNAi cassette directed to suppress the storage protein 2S albumin resulted in seeds with an 11-24 % elevation in overall protein. The phytoene synthase cassette alone produced enhanced ß-carotene content of an average 275 ± 6.10 µg/g dry seed and an overall altered seed composition of 11 % less protein and comparable nontransgenic amounts of both oil and carbohydrates. Stacking an RNAi to suppress the major 2S storage protein resulted in seeds that contain elevated protein and slight decrease in oil and carbohydrate amounts showing that Camelina rebalances its proteome within an enlarged protein content genotype. In both ß-carotene enhanced seeds with/without RNAi2S suppression, the seed size was noticeably enlarged compared to nontransgenic counterpart seeds. Metabolic analysis of maturing seeds indicate that the enhanced ß-carotene trait had the larger effect than the RNAi2S suppression on the seed metabolome. The use of a GRAS (generally regarded as safe) ß-carotene as a visual marker in a floral dip transformation system, such as Camelina, might eliminate the need for costly regulatory and controversial antibiotic resistance markers. ß-carotene enhanced RNAi2S suppressed Camelina seeds could be further developed as a rapid heterologous protein production platform in a nonfood crop leveraging its enlarged protein content and visual marker.
[Mh] Termos MeSH primário: Plantas Geneticamente Modificadas/genética
Proteoma/genética
Proteínas de Armazenamento de Sementes/genética
beta Caroteno/metabolismo
[Mh] Termos MeSH secundário: Brassicaceae/genética
Brassicaceae/crescimento & desenvolvimento
Ácidos Graxos/metabolismo
Genótipo
Óleos Vegetais/metabolismo
Plantas Geneticamente Modificadas/metabolismo
Proteínas de Armazenamento de Sementes/metabolismo
beta Caroteno/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Plant Oils); 0 (Proteome); 0 (Seed Storage Proteins); 01YAE03M7J (beta Carotene)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1007/s11248-016-9992-y


  4 / 1756 MEDLINE  
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[PMID]:28455403
[Au] Autor:Qiu Y; Liu SL; Adams KL
[Ad] Endereço:Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Y.Q., K.L.A.); and.
[Ti] Título:Concerted Divergence after Gene Duplication in Polycomb Repressive Complexes.
[So] Source:Plant Physiol;174(2):1192-1204, 2017 Jun.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Duplicated genes are a major contributor to genome evolution and phenotypic novelty. There are multiple possible evolutionary fates of duplicated genes. Here, we provide an example of concerted divergence of simultaneously duplicated genes whose products function in the same complex. We studied POLYCOMB REPRESSIVE COMPLEX2 (PRC2) in Brassicaceae. The VERNALIZATION (VRN)-PRC2 complex contains VRN2 and SWINGER (SWN), and both genes were duplicated during a whole-genome duplication to generate FERTILIZATION INDEPENDENT SEED2 (FIS2) and MEDEA (MEA), which function in the Brassicaceae-specific FIS-PRC2 complex that regulates seed development. We examined the expression of , , and their paralogs, compared their cytosine and histone methylation patterns, and analyzed the sequence evolution of the genes. We found that and have reproductive-specific expression patterns that are correlated and derived from the broadly expressed and in outgroup species. In vegetative tissues of Arabidopsis ( ), repressive methylation marks are enriched in and , whereas active marks are associated with their paralogs. We detected comparable accelerated amino acid substitution rates in and but not in their paralogs. We also show divergence patterns of the PRC2-associated that are similar to and These lines of evidence indicate that and have diverged in concert, resulting in functional divergence of the PRC2 complexes in Brassicaceae. This type of concerted divergence is a previously unreported fate of duplicated genes. In addition, the Brassicaceae-specific FIS-PRC2 complex modified the regulatory pathways in female gametophyte and seed development.
[Mh] Termos MeSH primário: Brassicaceae/genética
Duplicação Gênica
Variação Genética
Proteínas de Plantas/genética
Proteínas do Grupo Polycomb/genética
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Metilação de DNA/genética
Epigênese Genética
Evolução Molecular
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Histonas/metabolismo
Especificidade de Órgãos/genética
Proteínas de Plantas/metabolismo
Proteínas do Grupo Polycomb/metabolismo
Domínios e Motivos de Interação entre Proteínas
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histones); 0 (Plant Proteins); 0 (Polycomb-Group Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.01983


  5 / 1756 MEDLINE  
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[PMID]:27773670
[Au] Autor:Doucet J; Lee HK; Goring DR
[Ad] Endereço:Department of Cell and Systems Biology, University of Toronto, Toronto M5S 3B2, Canada.
[Ti] Título:Pollen Acceptance or Rejection: A Tale of Two Pathways.
[So] Source:Trends Plant Sci;21(12):1058-1067, 2016 Dec.
[Is] ISSN:1878-4372
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:While the molecular and cellular basis of self-incompatibility leading to self-pollen rejection in the Brassicaceae has been extensively studied, relatively little attention has been paid to compatible pollen recognition and the corresponding cellular responses in the stigmatic papillae. This is now changing because research has started to uncover steps in the Brassicaceae 'basal compatible pollen response pathway' in the stigma leading to pollen hydration and germination. Furthermore, recent studies suggest that self-incompatible pollen activates both the basal compatible pathway and the self-incompatibility pathway in the stigma, with the self-incompatibility response ultimately prevailing to reject self-pollen. We review here recent discoveries in both pathways and discuss how compatible pollen is accepted by the stigma versus the rejection of self-incompatible pollen.
[Mh] Termos MeSH primário: Brassicaceae/fisiologia
Pólen/fisiologia
[Mh] Termos MeSH secundário: Brassicaceae/metabolismo
Polinização/genética
Polinização/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  6 / 1756 MEDLINE  
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[PMID]:28912063
[Au] Autor:Liu L; Song Y; Xu J; Li D; Li G; An L
[Ad] Endereço:Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
[Ti] Título:Differential expression by chromatin modifications of alcohol dehydrogenase 1 of Chorispora bungeana in cold stress.
[So] Source:Gene;636:1-16, 2017 Dec 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Epigenetic modifications regulate plant genes to cope with a variety of environmental stresses. Chorispora bungeana is an alpine subnival plant with strong tolerance to multiple abiotic stresses, especially cold stress. In this study, we characterized the alcohol dehydrogenase 1 gene from Chorispora bungeana, CbADH1, that is up-regulated in cold conditions. Overexpression of CbADH1 in Arabidopsis thaliana improved cold tolerance, as indicated by a decreased lethal temperature (LT50). Chromatin immunoprecipitation assays showed that histone H3 is removed from the promoter region and the middle-coding region of the gene. H3K9 acetylation and H3K4 trimethylation increased throughout the gene and in the proximal promoter region, respectively. Moreover, increased Ser5P and Ser2P polymerase II accumulation further indicated changes in the transcription initiation and elongation of CbADH1 were due to the cold stress. Taken together, our results suggested that CbADH1 is highly expressed during cold stress, and is regulated by epigenetic modifications. This study expands our understanding of the regulation of gene expression by epigenetic modifications in response to environmental cues.
[Mh] Termos MeSH primário: Álcool Desidrogenase/genética
Brassicaceae/enzimologia
Resposta ao Choque Frio/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Álcool Desidrogenase/química
Álcool Desidrogenase/metabolismo
Sequência de Aminoácidos
Arabidopsis/genética
Brassicaceae/genética
Cromatina/metabolismo
Clonagem Molecular
Sequência Conservada
Histonas/metabolismo
Folhas de Planta/enzimologia
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
RNA Polimerase II/metabolismo
Elementos de Resposta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (Histones); 0 (Plant Proteins); EC 1.1.1.1 (Alcohol Dehydrogenase); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


  7 / 1756 MEDLINE  
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[PMID]:28859098
[Au] Autor:Zhao P; Wang L; Zhao X; Chen G; Ma XF
[Ad] Endereço:Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions, Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, P. R. China.
[Ti] Título:A comparative transcriptomic analysis reveals the core genetic components of salt and osmotic stress responses in Braya humilis.
[So] Source:PLoS One;12(8):e0183778, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Braya humilis is a member of the Euclidieae tribe within the family Brassicaceae. This species exhibits a broad range of adaptations to different climatic zones and latitudes as it has a distribution that ranges from northern Asia to the arctic-alpine regions of northern North America. In China, B. humilis is mainly found on the Qinghai-Tibetan Plateau (QTP) and in adjacent arid regions. In this study, we sequenced a sample from an arid region adjacent to the QTP using the Illumina platform generating a total of 46,485 highly accurate unigenes, of which 78.41% were annotated by BLASTing versus public protein databases. The B. humilis transcriptome is characterized by a high level of sequence conservation compared with its close relative, Arabidopsis thaliana. We also used reciprocal blast to identify shared orthologous genes between B. humilis and four other sequenced Brassicaceae species (i.e. A. thaliana, A. lyrata, Capsella rubella, and Thellungiella parvula). To enable precise characterization of orthologous genes, the early-diverging basal angiosperm Amborella trichopoda was also included. A total of 6,689 orthologous genes were identified before stricter criteria for the determination of e-values, amino acid hit lengths, and identity values was applied to further reduce this list. This led to a final list of 381 core orthologous genes for B. humilis; 39 out of these genes are involved in salt and osmotic stress responses and estimations of nonsynonymous/synonymous substitution ratios for this species and A. thaliana orthologs show that these genes are under purifying selection in B. humilis. Expression of six genes was detected in B. humilis seedlings under salt and osmotic stress treatments. Comparable expression patterns to their counterparts in Arabidopsis suggest that these orthologous genes are both sequence and functional conservation. The results of this study demonstrate that the environmental adaptations of B. humilis are mainly the results of preexisting genetic components. Future work will be required to characterize the expression patterns of these orthologous genes in natural populations and will provide further insights into the adaptive mechanisms underlying the wide range of B. humilis adaptations.
[Mh] Termos MeSH primário: Brassicaceae/genética
Evolução Molecular
Plantas Tolerantes a Sal/genética
Transcriptoma/genética
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
Arabidopsis/genética
Regiões Árticas
China
Clima Desértico
Regulação da Expressão Gênica de Plantas
Osmorregulação/genética
Plantas Tolerantes a Sal/crescimento & desenvolvimento
Cloreto de Sódio/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183778


  8 / 1756 MEDLINE  
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[PMID]:28830206
[Au] Autor:Lee IC; Kim DY; Bae JS
[Ad] Endereço:* Department of Cosmetic Science and Technology, Seowon University, Cheongju 28674, Republic of Korea.
[Ti] Título:Sulforaphane Reduces HMGB1-Mediated Septic Responses and Improves Survival Rate in Septic Mice.
[So] Source:Am J Chin Med;45(6):1253-1271, 2017.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Sulforaphane (SFN), a natural isothiocyanate present in cruciferous vegetables such as broccoli and cabbage, is effective in preventing carcinogenesis, diabetes, and inflammatory responses. Inhibition of high mobility group box 1 (HMGB1) and restoration of endothelial integrity is emerging as an attractive therapeutic strategy in the management of severe sepsis or septic shock. In this study, we examined the effects of SFN on HMGB1-mediated septic responses and survival rate in a mouse sepsis model. The anti-inflammatory activities of SFN were monitored based on its effects on lipopolysaccharide (LPS)- or cecal ligation and puncture (CLP)-mediated release of HMGB1. The antiseptic activities of SFN were determined by measuring permeability, leukocyte adhesion and migration, and the activation of pro-inflammatory proteins in HMGB1-activated human umbilical vein endothelial cells (HUVECs) and mice. SFN inhibited the release of HMGB1 and downregulated HMGB1-dependent inflammatory responses in human endothelial cells. SFN also inhibited HMGB1-mediated hyperpermeability and leukocyte migration in mice. In addition, treatment with SFN reduced CLP-induced release of HMGB1 and sepsis-related mortality and pulmonary injury in vivo. Our results indicate that SFN is a possible therapeutic agent that can be used to treat various severe vascular inflammatory diseases via the inhibition of the HMGB1 signaling pathway.
[Mh] Termos MeSH primário: Anti-Inflamatórios
Proteína HMGB1/antagonistas & inibidores
Proteína HMGB1/fisiologia
Mediadores da Inflamação/metabolismo
Mediadores da Inflamação/fisiologia
Isotiocianatos/farmacologia
Isotiocianatos/uso terapêutico
Fitoterapia
Sepse/tratamento farmacológico
Sepse/genética
Transdução de Sinais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Brassicaceae/química
Movimento Celular/efeitos dos fármacos
Modelos Animais de Doenças
Proteína HMGB1/metabolismo
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Leucócitos/imunologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (HMGB1 Protein); 0 (Inflammation Mediators); 0 (Isothiocyanates); GA49J4310U (sulforafan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X17500690


  9 / 1756 MEDLINE  
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[PMID]:28809950
[Au] Autor:Raman G; Park V; Kwak M; Lee B; Park S
[Ad] Endereço:Department of Life Sciences, Yeungnam University, Gyeongsan, Gyeongsan-buk, Republic of Korea.
[Ti] Título:Characterization of the complete chloroplast genome of Arabis stellari and comparisons with related species.
[So] Source:PLoS One;12(8):e0183197, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arabis stellari var. japonica is an ornamental plant of the Brassicaceae family, and is widely distributed in South Korea. However, no information is available about its molecular biology and no genomic study has been performed on A. stellari. In this paper, the authors report the complete chloroplast genome sequence of A. stellari. The plastome of A. stellari was 153,683 bp in length with 36.4% GC and included a pair of inverted repeats (IRs) of 26,423 bp that separated a large single-copy (LSC) region of 82,807 bp and a small single-copy (SSC) region of 18,030 bp. It was also found to contain 113 unique genes, of which 79 were protein-coding genes, 30 were transfer RNAs, and four were ribosomal RNAs. The gene content and organization of the A. stellari chloroplast genome were similar to those of other Brassicaceae genomes except for the absence of the rps16 protein-coding gene. A total of 991 SSRs were identified in the genome. The chloroplast genome of A. stellari was compared with closely related species of the Brassicaceae family. Comparative analysis showed a minor divergence occurred in the protein-coding matK, ycf1, ccsA, accD and rpl22 genes and that the KA/KS nucleotide substitution ratio of the ndhA genes of A. stellari and A. hirsuta was 1.35135. The genes infA and rps16 were absent in the Arabis genus and phylogenetic evolutionary studies revealed that these genes evolved independently. However, phylogenetic analysis showed that the positions of Brassicaceae species are highly conserved. The present study provides A. stellari genomic information that may be found useful in conservation and molecular phylogenetic studies on Brassicaceae.
[Mh] Termos MeSH primário: Arabis/genética
Genoma de Cloroplastos/genética
[Mh] Termos MeSH secundário: Brassicaceae/genética
Evolução Molecular
RNA Ribossômico/genética
República da Coreia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183197


  10 / 1756 MEDLINE  
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[PMID]:28732049
[Au] Autor:Keith RA; Mitchell-Olds T
[Ad] Endereço:University Program in Genetics and Genomics, Duke University, Durham, North Carolina, United States of America.
[Ti] Título:Testing the optimal defense hypothesis in nature: Variation for glucosinolate profiles within plants.
[So] Source:PLoS One;12(7):e0180971, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plants employ highly variable chemical defenses against a broad community of herbivores, which vary in their susceptibilities to specific compounds. Variation in chemical defenses within the plant has been found in many species; the ecological and evolutionary influences on this variation, however, are less well-understood. One central theory describing the allocation of defenses in the plant is the Optimal Defense Hypothesis (ODH), which predicts that defenses will be concentrated in tissues that are of high fitness value to the plant. Although the ODH has been repeatedly supported within vegetative tissues, few studies have compared vegetative and reproductive tissues, and the results have not been conclusive. We quantified variation in glucosinolate profile and tissue value between vegetative and reproductive tissues in Boechera stricta, a close relative of Arabidopsis. B. stricta manufactures glucosinolates, a set of defensive compounds that vary genetically and are straightforward to quantify. Genetic diversity in glucosinolate profile has been previously demonstrated to be important to both herbivory and fitness in B. stricta; however, the importance of glucosinolate variation among tissues has not. Here, we investigate whether allocation of glucosinolates within the plant is consistent with the ODH. We used both clipping experiments on endogenous plants and ambient herbivory in a large-scale transplant experiment at three sites to quantify fitness effects of loss of rosette leaves, cauline leaves, and flowers and fruits. We measured glucosinolate concentration in leaves and fruits in the transplant experiment, and asked whether more valuable tissues were more defended. We also investigated within-plant variation in other aspects of the glucosinolate profile. Our results indicated that damage to fruits had a significantly larger effect on overall fitness than damage to leaves, and that fruits had much higher concentrations of glucosinolates, supporting the ODH. This is, to the best of our knowledge, the first study to explicitly compare both tissue value and chemical defense concentrations between vegetative and reproductive tissues under natural conditions.
[Mh] Termos MeSH primário: Brassicaceae/metabolismo
Glucosinolatos/metabolismo
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Meio Ambiente
Flores/metabolismo
Frutas/metabolismo
Regulação da Expressão Gênica de Plantas/fisiologia
Herbivoria
Idaho
Modelos Biológicos
Análise Multivariada
Folhas de Planta/metabolismo
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucosinolates)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180971



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