Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.157.438 [Categoria DeCS]
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[PMID]:28622440
[Au] Autor:Miceli N; Filocamo A; Ragusa S; Cacciola F; Dugo P; Mondello L; Celano M; Maggisano V; Taviano MF
[Ad] Endereço:Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Polo Annunziata, Viale Annunziata, IT-98168, Messina.
[Ti] Título:Chemical Characterization and Biological Activities of Phenolic-Rich Fraction from Cauline Leaves of Isatis tinctoria L. (Brassicaceae) Growing in Sicily, Italy.
[So] Source:Chem Biodivers;14(8), 2017 Aug.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present work focused on the evaluation of the antioxidant and cytotoxic activities of the phenolic-rich fraction (ItJ-EAF) obtained from cauline leaves collected in January from Isatis tinctoria L. (Brassicaceae) growing wild around Acireale (Sicily, Italy). The total phenolic, flavonoid, and condensed tannin contents of the fraction were determined spectrophotometrically, whereas the phenolic profile was assessed by HPLC-PDA/ESI-MS analysis. A total of 20 compounds were positively identified and twelve out of them were never previously reported in I. tinctoria leaves. The fraction exhibited good radical scavenging activity in DPPH test (IC  = 0.6657 ± 0.0024 mg/ml) and reducing power (3.87 ± 0.71 ASE/ml), whereas, it neither showed chelating activity nor was able to counteract H O induced oxidative stress damage in Escherichia coli. The antiproliferative effect was evaluated in vitro on two human anaplastic thyroid carcinoma cell lines (CAL-62 and 8505C) by MTT assay. At the highest tested concentration ItJ-EAF significantly reduced (80%) the growth of CAL-62 cells. No cytotoxicity against Artemia salina was observed. It can be concluded that I. tinctoria cauline leaves represent a source of phenolic compounds which could be potentially used as chemopreventive or adjuvant agents against cancer.
[Mh] Termos MeSH primário: Isatis/química
Fenóis/química
Fenóis/farmacologia
Extratos Vegetais/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/química
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Cromatografia Líquida de Alta Pressão
Escherichia coli/efeitos dos fármacos
Escherichia coli/metabolismo
Seres Humanos
Peróxido de Hidrogênio/toxicidade
Isatis/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Fenóis/isolamento & purificação
Folhas de Planta/química
Folhas de Planta/metabolismo
Sicília
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Phenols); 0 (Plant Extracts); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201700073


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[PMID]:28475367
[Au] Autor:Meng LJ; Guo QL; Xu CB; Zhu CG; Liu YF; Chen MH; Lin S; Li YH; Jiang JD; Shi JG
[Ad] Endereço:a State Key Laboratory of Bioactive Substance and Function of Natural Medicines , Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050 , China.
[Ti] Título:Diglycosidic indole alkaloid derivatives from an aqueous extract of Isatis indigotica roots.
[So] Source:J Asian Nat Prod Res;19(6):529-540, 2017 Jun.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Six new indole alkaloid diglycosides named isatigotindolediosides A-F (1-6), along with three known analogs (7-9), were isolated from an aqueous extract of the Isatis indigotica roots (ban lan gen). Their structures including the absolute configurations were determined by comprehensive spectroscopic data analysis, combined with enzyme or acid hydrolysis, and comparison of experimental circular dichroism (CD) and calculated electronic circular dichroism (ECD) spectra. In the preliminary assays, compounds 3, 5, and 8 showed antiviral activity against Coxsackie virus B3.
[Mh] Termos MeSH primário: Glicosídeos/isolamento & purificação
Alcaloides de Indol/isolamento & purificação
Isatis/química
Raízes de Plantas/química
[Mh] Termos MeSH secundário: Antivirais/química
Antivirais/isolamento & purificação
Antivirais/farmacologia
Dicroísmo Circular
Enterovirus Humano B/efeitos dos fármacos
Glicosídeos/química
Glicosídeos/farmacologia
Alcaloides de Indol/química
Alcaloides de Indol/farmacologia
Estrutura Molecular
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Glycosides); 0 (Indole Alkaloids); 059QF0KO0R (Water)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2017.1320547


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[PMID]:27919254
[Au] Autor:Zhao X; He X; Zhong X
[Ad] Endereço:College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, No. 2596 Lekai South Street, Baoding, Hebei, 071000, People's Republic of China. xianzhaoxinghua@163.com.
[Ti] Título:Anti-inflammatory and in-vitro antibacterial activities of Traditional Chinese Medicine Formula Qingdaisan.
[So] Source:BMC Complement Altern Med;16(1):503, 2016 Dec 05.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Qingdaisan (Formulated Indigo powder, QDS) are widely used for treatment of aphtha, sore throat and bleeding gums in China. The aim of the study is to evaluate the anti-inflammatory, antibacterial and dental ulcer therapeutic effects of QDS. METHODS: Dimethylbenzene-induced ear edema test and cotton pellet-induced granuloma test were used to evaluate anti-inflammatory activities of QDS on acute and chronic inflammatory. The healing time and local pathologic changes were used to assess the therapeutic effects of QDS on dental ulcer. The antibacterial activities of each component and the whole formulation of QDS were determined by agar well diffusion assay. High-dose and low-dose QDS were tested in this experiment and Gui Lin Watermelon Frost Powder (GLWFP) was used as positive control. RESULTS: Oral treatment with QDS significantly accelerated the healing of ulcerative lesions induced by phenol injury. The dental ulcers of high-dose QDS group were all healed within 6 days. It was shorter than those of low-dose QDS group and GLWFP group. Less quantity of inflammatory cells and plenty fibroblasts were observed in pathological section of QDS groups. QDS also exhibited significant anti-inflammatory activity both in acute and chronic animal models. Although some of the components exhibited antibacterial activities, the whole formulation of QDS didn't show any significant antibacterial activity in vitro. CONCLUSION: The study showed that QDS has obviously anti-inflammatory activity for both acute and chronic inflammatory, also has a remarkable effect for healing dental ulcer caused by phenol. QDS didn't have antibacterial activity to selected strains in vitro.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Anti-Inflamatórios/farmacologia
Medicamentos de Ervas Chinesas/farmacologia
[Mh] Termos MeSH secundário: Animais
Medicamentos de Ervas Chinesas/uso terapêutico
Isatis/química
Masculino
Camundongos
Testes de Sensibilidade Microbiana
Úlceras Orais/tratamento farmacológico
Úlceras Orais/patologia
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Inflammatory Agents); 0 (Drugs, Chinese Herbal); 0 (Qingdai compound)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161207
[St] Status:MEDLINE


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[PMID]:27914524
[Au] Autor:Ma RF; Liu QZ; Xiao Y; Zhang L; Li Q; Yin J; Chen WS
[Ad] Endereço:Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
[Ti] Título:The phenylalanine ammonia-lyase gene family in Isatis indigotica Fort.: molecular cloning, characterization, and expression analysis.
[So] Source:Chin J Nat Med;14(11):801-812, 2016 Nov.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Phenolic compounds, metabolites of the phenylpropanoid pathway, play an important role in the growth and environmental adaptation of many plants. Phenylalanine ammonia-lyase (PAL) is the first key enzyme of the phenylpropanoid pathway. The present study was designed to investigate whether there is a multi-gene family in I. Indigotic and, if so, to characterize their properties. We conducted a comprehensive survey on the transcription profiling database by using tBLASTn analysis. Several bioinformatics methods were employed to perform the prediction of composition and physicochemical characters. The expression levels of IiPAL genes in various tissues of I. indigotica with stress treatment were examined by quantitative real-time PCR. Protoplast transient transformation was used to observe the locations of IiPALs. IiPALs were functionally characterized by expression with pET-32a vector in Escherichia colis strain BL21 (DE3). Integration of transcripts and metabolite accumulations was used to reveal the relation between IiPALs and target compounds. An new gene (IiPAL2) was identified and both IiPALs had the conserved enzymatic active site Ala-Ser-Gly and were classified as members of dicotyledon. IiPAL1 and IiPAL2 were expressed in roots, stems, leaves, and flowers, with the highest expression levels of IiPAL1 and IiPAL2 being observed in stems and roots, respectively. The two genes responded to the exogenous elicitor in different manners. Subcellular localization experiment showed that both IiPALs were localized in the cytosol. The recombinant proteins were shown to catalyze the conversion of L-Phe to trans-cinnamic acid. Correlation analysis indicated that IiPAL1 was more close to the biosynthesis of secondary metabolites than IiPAL2. In conclusion, the present study provides a basis for the elucidation of the role of IiPALs genes in the biosynthesis of phenolic compounds, which will help further metabolic engineering to improve the accumulation of bioactive components in I. indigotica.
[Mh] Termos MeSH primário: Clonagem Molecular
Isatis/enzimologia
Fenilalanina Amônia-Liase/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Regulação da Expressão Gênica de Plantas
Isatis/genética
Dados de Sequência Molecular
Família Multigênica
Fenilalanina Amônia-Liase/química
Fenilalanina Amônia-Liase/metabolismo
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); EC 4.3.1.24 (Phenylalanine Ammonia-Lyase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161205
[St] Status:MEDLINE


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[PMID]:27420350
[Au] Autor:Jähne EA; Eigenmann DE; Moradi-Afrapoli F; Verjee S; Butterweck V; Hebeisen S; Hettich T; Schlotterbeck G; Smiesko M; Hamburger M; Oufir M
[Ad] Endereço:Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland.
[Ti] Título:Caco-2 Permeability Studies and In Vitro hERG Liability Assessment of Tryptanthrin and Indolinone.
[So] Source:Planta Med;82(13):1192-201, 2016 Aug.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Tryptanthrin and (E,Z)-3-(4-hydroxy-3,5-dimethoxybenzylidene)indolinone (indolinone) were recently isolated from Isatis tinctoria as potent anti-inflammatory and antiallergic alkaloids, and shown to inhibit COX-2, 5-LOX catalyzed leukotriene synthesis, and mast cell degranulation at low µM to nM concentrations. To assess their suitability for oral administration, we screened the compounds in an in vitro intestinal permeability assay using human colonic adenocarcinoma cells. For exact quantification of the compounds, validated UPLC-MS/MS methods were used. Tryptanthrin displayed high permeability (apparent permeability coefficient > 32.0 × 10(-6) cm/s) across the cell monolayer. The efflux ratio below 2 (< 1.12) and unchanged apparent permeability coefficient values in the presence of the P-glycoprotein inhibitor verapamil (50 µM) indicated that tryptanthrin was not involved in P-glycoprotein interactions. For indolinone, a low recovery was found in the human colon adenocarcinoma cell assay. High-resolution mass spectrometry pointed to extensive phase II metabolism of indolinone (sulfation and glucuronidation). Possible cardiotoxic liability of the compounds was assessed in vitro by measurement of an inhibitory effect on human ether-a-go-go-related gene tail currents in stably transfected HEK 293 cells using the patch clamp technique. Low human ether-a-go-go-related gene inhibition was found for tryptanthrin (IC50 > 10 µM) and indolinone (IC50 of 24.96 µM). The analysis of compounds using various in silico methods confirmed favorable pharmacokinetic properties, as well as a slight inhibition of the human ether-a-go-go-related gene potassium channel at micromolar concentrations.
[Mh] Termos MeSH primário: Antialérgicos/farmacocinética
Anti-Inflamatórios não Esteroides/farmacocinética
Indóis/farmacocinética
Pirogalol/análogos & derivados
Quinazolinas/farmacocinética
[Mh] Termos MeSH secundário: Células CACO-2
Permeabilidade da Membrana Celular
Cromatografia Líquida de Alta Pressão/métodos
Células HEK293
Seres Humanos
Absorção Intestinal
Isatis/química
Pirogalol/farmacocinética
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3-(4-hydroxy-3,5-dimethoxybenzylidene)indolinone); 0 (Anti-Allergic Agents); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Indoles); 0 (Indolinone A); 0 (Quinazolines); 01Y4A2QXY0 (Pyrogallol); 13220-57-0 (tryptanthrine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160716
[St] Status:MEDLINE
[do] DOI:10.1055/s-0042-110323


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[PMID]:27203567
[Au] Autor:Ahmad A; Wei Y; Syed F; Khan S; Khan GM; Tahir K; Khan AU; Raza M; Khan FU; Yuan Q
[Ad] Endereço:State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, No. 15 East Road of North Third Ring, Chao Yang District, Beijing 100029, China. Electronic address: aftabbiochem@yahoo.com.
[Ti] Título:Isatis tinctoria mediated synthesis of amphotericin B-bound silver nanoparticles with enhanced photoinduced antileishmanial activity: A novel green approach.
[So] Source:J Photochem Photobiol B;161:17-24, 2016 Aug.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:After malaria, Leishmaniasis is the most prevalent infectious disease in terms of fatality and geographical distribution. The availability of a limited number of antileishmanial agents, emerging resistance to the available drugs, and the high cost of treatment complicate the treatment of leishmaniasis. To overcome these issues, critical research for new therapeutic agents with enhanced antileishmanial potential and low treatment cost is needed. In this contribution, we developed a green protocol to prepare biogenic silver nanoparticles (AgNPs) and amphotericin B-bound biogenic silver nanoparticles (AmB-AgNPs). Phytochemicals from the aqueous extract of Isatis tinctoria were used as reducing and capping agents to prepare silver nanoparticles. Amphotericin B was successfully adsorbed on the surface of biogenic silver nanoparticles. The prepared nanoparticles were characterized by various analytical techniques. UV-Visible spectroscopy was employed to detect the characteristic localized surface plasmon resonance peaks (LSPR) for the prepared nanoparticles. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) studies revealed the formation of spherical silver nanoparticles with an average particle size of 10-20nm. The cubic crystalline structure of the prepared nanoparticles was confirmed by X-ray diffraction (XRD) study. FTIR spectroscopic analysis revealed that plant polyphenolic compounds are mainly involved in metal reduction and capping. Under visible light irradiation, biogenic silver nanoparticles exhibited significant activity against Leishmania tropica with an IC50 value of 4.2µg/mL. The leishmanicidal activity of these nanoparticles was considerably enhanced by conjugation with amphotericin B (IC50=2.43µg/mL). In conclusion, the findings of this study reveal that adsorption of amphotericin B, an antileishmanial drug, to biogenic silver nanoparticles, could be a safe, more effective and economic alternative to the available antileishmanial strategies.
[Mh] Termos MeSH primário: Anfotericina B/química
Antiprotozoários/síntese química
Isatis/química
Nanopartículas Metálicas/química
Prata/química
[Mh] Termos MeSH secundário: Anfotericina B/farmacologia
Antiprotozoários/química
Antiprotozoários/farmacologia
Química Verde
Isatis/metabolismo
Leishmania tropica/efeitos dos fármacos
Nanopartículas Metálicas/toxicidade
Tamanho da Partícula
Extratos Vegetais/química
Folhas de Planta/química
Folhas de Planta/metabolismo
Espectrofotometria Ultravioleta
Espectroscopia de Infravermelho com Transformada de Fourier
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Plant Extracts); 3M4G523W1G (Silver); 7XU7A7DROE (Amphotericin B)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160521
[St] Status:MEDLINE


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[PMID]:27155368
[Au] Autor:Zhang L; Chen J; Zhou X; Chen X; Li Q; Tan H; Dong X; Xiao Y; Chen L; Chen W
[Ad] Endereço:Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, China.
[Ti] Título:Dynamic metabolic and transcriptomic profiling of methyl jasmonate-treated hairy roots reveals synthetic characters and regulators of lignan biosynthesis in Isatis indigotica Fort.
[So] Source:Plant Biotechnol J;14(12):2217-2227, 2016 Dec.
[Is] ISSN:1467-7652
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A molecular description of lignan biosynthesis in Isatis indigotica displaying its synthetic characteristics and regulatory mechanism is of great importance for the improvement of the production of this class of active compounds. To discover the potential key catalytic steps and regulatory genes, I. indigotica hairy roots elicited by methyl jasmonate (MeJA) were used as a source of systematic variation for exploring the metabolic/transcriptional changes and candidate genes that might play key roles in lignan biosynthesis. The reprogramming modulated by MeJA was classified into three distinct phases, referred to as signal responding, transcriptional activation of metabolic pathways and accumulation of metabolites. Candidate genes were pooled according to the three phases and applied to co-expression network analysis. In total, 17 genes were identified as hub genes. 4CL3 was selected to validate its impact on lignan biosynthesis. RNAi of 4CL3 resulted in a significant reduction in lignan production. Taken together with its catalytic property, a major route of lignan biosynthesis in I. indigotica was highlighted, which was catalysed by 4CL3 via the esterization of caffeic acid. In conclusion, this study provides new insights into lignan biosynthesis as well as potential targets for metabolic engineering in I. indigotica.
[Mh] Termos MeSH primário: Acetatos/farmacologia
Ciclopentanos/farmacologia
Isatis/metabolismo
Lignanas/biossíntese
Oxilipinas/farmacologia
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/genética
Metaboloma
Reguladores de Crescimento de Planta/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Cyclopentanes); 0 (Lignans); 0 (Oxylipins); 0 (Plant Growth Regulators); 900N171A0F (methyl jasmonate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160508
[St] Status:MEDLINE
[do] DOI:10.1111/pbi.12576


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[PMID]:27093249
[Au] Autor:Jähne EA; Eigenmann DE; Sampath C; Butterweck V; Culot M; Cecchelli R; Gosselet F; Walter FR; Deli MA; Smiesko M; Hamburger M; Oufir M
[Ad] Endereço:Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland.
[Ti] Título:Pharmacokinetics and In Vitro Blood-Brain Barrier Screening of the Plant-Derived Alkaloid Tryptanthrin.
[So] Source:Planta Med;82(11-12):1021-9, 2016 Jul.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The indolo[2,1-b]quinazoline alkaloid tryptanthrin was previously identified as a potent anti-inflammatory compound with a unique pharmacological profile. It is a potent inhibitor of cyclooxygenase-2, 5-lipooxygenase-catalyzed leukotriene synthesis, and nitric oxide production catalyzed by the inducible nitric oxide synthase. To characterize the pharmacokinetic properties of tryptanthrin, we performed a pilot in vivo study in male Sprague-Dawley rats (2 mg/kg bw i. v.). Moreover, the ability of tryptanthrin to cross the blood-brain barrier was evaluated in three in vitro human and animal blood-brain barrier models. Bioanalytical UPLC-MS/MS methods used were validated according to current international guidelines. A half-life of 40.63 ± 6.66 min and a clearance of 1.00 ± 0.36 L/h/kg were found in the in vivo pharmacokinetic study. In vitro data obtained with the two primary animal blood-brain barrier models showed a good correlation with an immortalized human monoculture blood-brain barrier model (hBMEC cell line), and were indicative of a high blood-brain barrier permeation potential of tryptanthrin. These findings were corroborated by the in silico prediction of blood-brain barrier penetration. P-glycoprotein interaction of tryptanthrin was assessed by calculation of the efflux ratio in bidirectional permeability assays. An efflux ratio below 2 indicated that tryptanthrin is not subjected to active efflux.
[Mh] Termos MeSH primário: Barreira Hematoencefálica/metabolismo
Quinazolinas/farmacocinética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cromatografia Líquida de Alta Pressão/métodos
Seres Humanos
Isatis/química
Masculino
Estrutura Molecular
Extratos Vegetais/farmacocinética
Quinazolinas/síntese química
Quinazolinas/química
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Quinazolines); 13220-57-0 (tryptanthrine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160420
[St] Status:MEDLINE
[do] DOI:10.1055/s-0042-105295


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[PMID]:27025363
[Au] Autor:Zhang L; Li Q; Chen JF; Chen WS
[Ad] Endereço:Department of Pharmacy, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
[Ti] Título:Computational identification and systematic classification of novel GRAS genes in Isatis indigotica.
[So] Source:Chin J Nat Med;14(3):161-76, 2016 Mar.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Isatis indigotica Fort., belonging to Cruciferae, is one of the most commonly used plants in traditional Chinese medicine. The accumulation of the effective components of I. indigotica is related with its growth conditions. The GRAS genes are members of a multigene family of transcriptional regulators that play a crucial role in plant growth. Although the activities of many GRAS genes have long been recognized, only in recent years were some of them identified and functionally characterized in detail. In the present study, 41 GRAS genes were identified from I. indigotica through bioinformatics methods for the first time. They were classified into ten groups according to the classification of Arabidopsis and rice. The characterization, gene structure, conserved motifs, disordered N-terminal domains, and phylogenetic reconstruction of these GRASs were analyzed. Forty-three orthologous gene pairs were shared by I. indigotica and Arabidopsis, and interaction networks of these orthologous genes were constructed. Furthermore, gene expression patterns were investigated by analysis in methyl jasmonate (MeJA)-treated I. indigotica hairy roots based on RNA-seq data. In conclusion, this comprehensive analysis would provide rich resources for further studies of GRAS protein functions in this plant.
[Mh] Termos MeSH primário: Genes de Plantas
Isatis/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Biologia Computacional
Perfilação da Expressão Gênica
Medicina Tradicional Chinesa
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Transcription Factors)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160331
[St] Status:MEDLINE


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[PMID]:26875535
[Au] Autor:Zhang W; Zheng X; Cheng N; Gai W; Xue X; Wang Y; Gao Y; Shan J; Yang S; Xia X
[Ad] Endereço:College of Veterinary Medicine, Jilin University, Changchun, China; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China.
[Ti] Título:Isatis indigotica root polysaccharides as adjuvants for an inactivated rabies virus vaccine.
[So] Source:Int J Biol Macromol;87:7-15, 2016 Jun.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Adjuvants can enhance vaccine immunogenicity and induce long-term enhancement of immune responses. Thus, adjuvants are important for vaccine research. Polysaccharides isolated from select Chinese herbs have been demonstrated to possess various beneficial functions and excellent adjuvant abilities. In the present study, the polysaccharides IIP-A-1 and IIP-2 were isolated from Isatis indigotica root and compared with the common vaccine adjuvant aluminum hydroxide via intramuscular co-administration of inactivated rabies virus rCVS-11-G into mice. Blood was collected to determine virus neutralizing antibody (VNA) titers and B and T lymphocyte activation status. Inguinal lymph node samples were collected and used to measure B lymphocyte proliferation. Splenocytes were isolated, from which antigen-specific cellular immune responses were detected via ELISpot, ELISA and intracellular cytokine staining. The results revealed that both types of polysaccharides induce more rapid changes and higher VNA titers than aluminum hydroxide. Flow cytometry assays revealed that the polysaccharides activated more B lymphocytes in the lymph nodes and more B and T lymphocytes in the blood than aluminum hydroxide. Antigen-specific cellular immune responses showed that IIP-2 strongly induced T lymphocyte proliferation in the spleen and high levels of cytokine secretion from splenocytes, whereas aluminum hydroxide induced proliferation in only a small number of lymphocytes and the secretion of only small quantities of cytokines. Collectively, these data suggest that the polysaccharide IIP-2 exhibits excellent adjuvant activity and can enhance both cellular and humoral immunity.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/farmacologia
Isatis/química
Raízes de Plantas/química
Polissacarídeos/farmacologia
Vacinas Antirrábicas/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/sangue
Antígenos Virais/imunologia
Linfócitos B/efeitos dos fármacos
Linfócitos B/imunologia
Citocinas/secreção
Feminino
Imunidade Celular/efeitos dos fármacos
Imunidade Humoral/efeitos dos fármacos
Camundongos
Camundongos Endogâmicos BALB C
Vírus da Raiva/efeitos dos fármacos
Vírus da Raiva/imunologia
Vírus da Raiva/patogenicidade
Baço/imunologia
Linfócitos T/efeitos dos fármacos
Linfócitos T/imunologia
Vacinas de Produtos Inativados/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Neutralizing); 0 (Antigens, Viral); 0 (Cytokines); 0 (Polysaccharides); 0 (Rabies Vaccines); 0 (Vaccines, Inactivated)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160216
[St] Status:MEDLINE



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