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[PMID]:28840805
[Au] Autor:Vicente JG; Rothwell S; Holub EB; Studholme DJ
[Ad] Endereço:1​School of Life Sciences, The University of Warwick, Wellesbourne, Warwick CV35 9EF, UK.
[Ti] Título:Pathogenic, phenotypic and molecular characterisation of Xanthomonas nasturtii sp. nov. and Xanthomonas floridensis sp. nov., new species of Xanthomonas associated with watercress production in Florida.
[So] Source:Int J Syst Evol Microbiol;67(9):3645-3654, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We describe two new species of the genus Xanthomonas, represented by yellow mucoid bacterial strains isolated from diseased leaves of watercress (Nasturtium officinale) produced in Florida, USA. One strain was pathogenic on watercress, but not in other species including a range of brassicas; other strains were not pathogenic in any of the tested plants. Data from Biolog carbon source utilization tests and nucleotide sequence data from 16S and gyrB loci suggested that both pathogenic and non-pathogenic strains were related to, yet distinct from, previously described Xanthomonas species. Multilocus sequence analysis and whole genome-wide comparisons of the average nucleotide identity (ANI) of genomes of two strains from watercress showed that these are distinct and share less than 95 % ANI with all other known species; the non-pathogenic strain WHRI 8848 is close to Xanthomonascassavae (ANI of 93.72 %) whilst the pathogenic strain WHRI 8853 is close to a large clade of species that includes Xanthomonasvesicatoria (ANI ≤90.25 %). Based on these results, we propose that both strains represent new Xanthomonas species named Xanthomonas floridensis sp. nov. (type strain WHRI 8848=ATCC TSD-60=ICMP 21312=LMG 29665=NCPPB 4601) and Xanthomonas nasturtii sp. nov. (type strain WHRI 8853=ATCC TSD-61=ICMP 21313=LMG 29666=NCPPB 4600), respectively. The presence of non-pathogenic Xanthomonas strains in watercress and their interaction with pathogenic strains needs to be further investigated. Although the importance of the new pathogenic species is yet to be determined, the bacterial disease that it causes constitutes a threat to watercress production and its distribution should be monitored.
[Mh] Termos MeSH primário: Nasturtium/microbiologia
Filogenia
Doenças das Plantas/microbiologia
Folhas de Planta/microbiologia
Xanthomonas/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Florida
Genes Bacterianos
Tipagem de Sequências Multilocus
Hibridização de Ácido Nucleico
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Xanthomonas/genética
Xanthomonas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002189


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[PMID]:28681323
[Au] Autor:Li K; Lin L; Wang J; Xia H; Liang D; Wang X; Liao M; Wang L; Liu L; Chen C; Tang Y
[Ad] Endereço:College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
[Ti] Título:Hyperaccumulator straw improves the cadmium phytoextraction efficiency of emergent plant Nasturtium officinale.
[So] Source:Environ Monit Assess;189(8):374, 2017 Aug.
[Is] ISSN:1573-2959
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:With the development of economy, the heavy metal contamination has become an increasingly serious problem, especially the cadmium (Cd) contamination. The emergent plant Nasturtium officinale R. Br. is a Cd-accumulator with low phytoremediation ability. To improve Cd phytoextraction efficiency of N. officinale, the straw from Cd-hyperaccumulator plants Youngia erythrocarpa, Galinsoga parviflora, Siegesbeckia orientalis, and Bidens pilosa was applied to Cd-contaminated soil and N. officinale was then planted; the study assessed the effect of hyperaccumulator straw on the growth and Cd accumulation of N. officinale. The results showed that application of hyperaccumulator species straws increased the biomass and photosynthetic pigment content and reduced the root/shoot ratio of N. officinale. All straw treatments significantly increased Cd content in roots, but significantly decreased Cd content in shoots of N. officinale. Applying hyperaccumulator straw significantly increased the total Cd accumulation in the roots, shoots, and whole plants of N. officinale. Therefore, application of straw from four hyperaccumulator species promoted the growth of N. officinale and improved the phytoextraction efficiency of N. officinale in Cd-contaminated paddy field soil; the straw of Y. erythrocarpa provided the most improvement.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Cádmio/análise
Poluentes do Solo/análise
[Mh] Termos MeSH secundário: Biomassa
Monitoramento Ambiental
Metais Pesados
Nasturtium/química
Raízes de Plantas/química
Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals, Heavy); 0 (Soil); 0 (Soil Pollutants); 00BH33GNGH (Cadmium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1007/s10661-017-6106-0


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[PMID]:28394276
[Au] Autor:Pereira LP; Silva P; Duarte M; Rodrigues L; Duarte CM; Albuquerque C; Serra AT
[Ad] Endereço:iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal. lu.pebre.pereira@gmail.com.
[Ti] Título:Targeting Colorectal Cancer Proliferation, Stemness and Metastatic Potential Using Brassicaceae Extracts Enriched in Isothiocyanates: A 3D Cell Model-Based Study.
[So] Source:Nutrients;9(4), 2017 Apr 10.
[Is] ISSN:2072-6643
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Colorectal cancer (CRC) recurrence is often attributable to circulating tumor cells and/or cancer stem cells (CSCs) that resist to conventional therapies and foster tumor progression. Isothiocyanates (ITCs) derived from vegetables have demonstrated anticancer effects in CRC, however little is known about their effect in CSCs and tumor initiation properties. Here we examined the effect of ITCs-enriched extracts derived from watercress and broccoli in cell proliferation, CSC phenotype and metastasis using a previously developed three-dimensional HT29 cell model with CSC-like traits. Both extracts were phytochemically characterized and their antiproliferative effect in HT29 monolayers was explored. Next, we performed cell proliferation assays and flow cytometry analysis in HT29 spheroids treated with watercress and broccoli extracts and respective main ITCs, phenethyl isothiocyanate (PEITC) and sulforaphane (SFN). Soft agar assays and relative quantitative expression analysis of stemness markers and Wnt/ß-catenin signaling players were performed to evaluate the effect of these phytochemicals in stemness and metastasis. Our results showed that both extracts and ITCs exert antiproliferative effects in HT29 spheroids, arresting cell cycle at G2/M, possibly due to ITC-induced DNA damage. Colony formation and expression of LGR5 and CD133 cancer stemness markers were significantly reduced. Only watercress extract and PEITC decreased ALDH1 activity in a dose-dependent manner, as well as ß-catenin expression. Our research provides new insights on CRC therapy using ITC-enriched extracts, specially watercress extract, to target CSCs and circulating tumor cells by impairing cell proliferation, ALDH1-mediated chemo-resistance, anoikis evasion, self-renewal and metastatic potential.
[Mh] Termos MeSH primário: Anticarcinógenos/metabolismo
Brassica/química
Neoplasias Colorretais/prevenção & controle
Isotiocianatos/metabolismo
Nasturtium/química
Metástase Neoplásica/prevenção & controle
Extratos Vegetais/metabolismo
[Mh] Termos MeSH secundário: Anticarcinógenos/análise
Anticarcinógenos/química
Anticarcinógenos/isolamento & purificação
Antineoplásicos Fitogênicos/análise
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/metabolismo
Biomarcadores Tumorais/genética
Biomarcadores Tumorais/metabolismo
Brassica/economia
Células CACO-2
Dióxido de Carbono/química
Diferenciação Celular
Proliferação Celular
Neoplasias Colorretais/dietoterapia
Neoplasias Colorretais/metabolismo
Neoplasias Colorretais/patologia
Suplementos Nutricionais/análise
Resistência a Medicamentos Antineoplásicos
Regulação Neoplásica da Expressão Gênica
Células HT29
Seres Humanos
Isotiocianatos/análise
Isotiocianatos/isolamento & purificação
Metástase Neoplásica/patologia
Metástase Neoplásica/terapia
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Solventes/química
Esferoides Celulares
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anticarcinogenic Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Biomarkers, Tumor); 0 (Isothiocyanates); 0 (Plant Extracts); 0 (Solvents); 142M471B3J (Carbon Dioxide); 6U7TFK75KV (phenethyl isothiocyanate); GA49J4310U (sulforafan)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE


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[PMID]:28390240
[Au] Autor:Pedras MSC; To QH
[Ad] Endereço:Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK, S7N 5C9, Canada. Electronic address: s.pedras@usask.ca.
[Ti] Título:Defense and signalling metabolites of the crucifer Erucastrum canariense: Synchronized abiotic induction of phytoalexins and galacto-oxylipins.
[So] Source:Phytochemistry;139:18-24, 2017 Jul.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Erucastrum canariense Webb & Berthel. (Brassicaceae) is a wild crucifer that grows in rocky soils, in salt and water stressed habitats, namely in the Canary Islands and similar environments. Abiotic stress induced by copper chloride triggered formation of a phytoalexin and galacto-oxylipins in E. canariense, whereas wounding induced galacto-oxylipins but not phytoalexins. Analysis of the metabolite profiles of leaves of E. canariense followed by isolation and structure determination afforded the phytoalexin erucalexin, the phytoanticipin indolyl-3-acetonitrile, the galacto-oxylipins arabidopsides A, C, and D, and the oxylipin 12-oxophytodienoic acid. In addition, arabidopsides A and D were also identified in extracts of leaves of Nasturtium officinale R. Br.
[Mh] Termos MeSH primário: Antifúngicos/isolamento & purificação
Brassicaceae/química
Oxilipinas/metabolismo
Sesquiterpenos/metabolismo
[Mh] Termos MeSH secundário: Antifúngicos/química
Brassicaceae/crescimento & desenvolvimento
Cromatografia Líquida de Alta Pressão
Ácidos Graxos Insaturados/química
Ácidos Graxos Insaturados/metabolismo
Indóis/análise
Indóis/química
Indóis/isolamento & purificação
Estrutura Molecular
Nasturtium/química
Ressonância Magnética Nuclear Biomolecular
Folhas de Planta/química
Sesquiterpenos/farmacologia
Espanha
Compostos de Espiro/análise
Compostos de Espiro/química
Compostos de Espiro/isolamento & purificação
Tiazóis/análise
Tiazóis/química
Tiazóis/isolamento & purificação
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Fatty Acids, Unsaturated); 0 (Indoles); 0 (Oxylipins); 0 (Sesquiterpenes); 0 (Spiro Compounds); 0 (Thiazoles); 0 (erucalexin); 37297-20-4 (phytoalexins); 67204-66-4 (12-oxophytodienoic acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE


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[PMID]:27374550
[Au] Autor:Giallourou N; Oruna-Concha MJ; Harbourne N
[Ad] Endereço:Department of Food and Nutritional Sciences, School of Chemistry, Food and Pharmacy, University of Reading, Whiteknights Campus, Reading, United Kingdom. Electronic address: n.giallourou@pgr.reading.ac.uk.
[Ti] Título:Effects of domestic processing methods on the phytochemical content of watercress (Nasturtium officinale).
[So] Source:Food Chem;212:411-9, 2016 Dec 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The impact of conventional cooking and processing methods on total phenols, antioxidant activity, carotenoids and glucosinolates of watercress was evaluated. Boiling significantly decreases phenolic content, antioxidant activity and recoverable glucosinolates, however it increases the carotenoid concentrations of watercress as compared to the raw vegetable. Cooking by microwaving and steaming maintains the majority of phytochemicals in comparison to the fresh material, suggesting that they should be used as the preferred methods of watercress preparation. Boiling of watercress should be avoided to ensure maximum ingestion of watercress-derived beneficial phytochemicals.
[Mh] Termos MeSH primário: Nasturtium/química
Compostos Fitoquímicos/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Antioxidantes/análise
Antioxidantes/química
Carotenoides/análise
Carotenoides/química
Culinária/métodos
Glucosinolatos/análise
Glucosinolatos/química
Micro-Ondas
Fenóis/análise
Fenóis/química
Compostos Fitoquímicos/análise
Extratos Vegetais/análise
Vapor
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Glucosinolates); 0 (Phenols); 0 (Phytochemicals); 0 (Plant Extracts); 0 (Steam); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE


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[PMID]:27313156
[Au] Autor:Gonda S; Kiss-Szikszai A; Szucs Z; Nguyen NM; Vasas G
[Ad] Endereço:Department of Botany, Division of Pharmacognosy, University of Debrecen, Egyetem tér 1, H-4010, Debrecen, Hungary.
[Ti] Título:Myrosinase Compatible Simultaneous Determination of Glucosinolates and Allyl Isothiocyanate by Capillary Electrophoresis Micellar Electrokinetic Chromatography (CE-MEKC).
[So] Source:Phytochem Anal;27(3-4):191-8, 2016 May.
[Is] ISSN:1099-1565
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: The functional food Cruciferous vegetables contain glucosinolates which are decomposed by the myrosinase enzyme upon tissue damage. The isothiocyanates are the most frequent decomposition products. Because of their various bioactivities, these compounds and the myrosinase is of high interest to many scientific fields. OBJECTIVE: Development of a capillary electrophoresis method capable of myrosinase-compatible, simultaneous quantification of glucosinolates and isothiocyanates. METHODS: Capillary electrochromatography parameters were optimised, followed by optimisation of a myrosinase-compatible derivatisation procedure for isothiocyanates. Vegetable extracts (Brussels sprouts, horseradish, radish and watercress) were tested for myrosinase activity, glucosinolate content and isothiocyanate conversion rate. Allyl isothiocyanate was quantified in some food products. RESULTS: The method allows quantification of sinigrin, gluonasturtiin and allyl isothiocyanate after myrosinase compatible derivatisation in-vial by mercaptoacetic acid. The chromatograhpic separation takes 2.5 min (short-end injection) or 15 min (long-end injection). For the tested vegetables, measured myrosinase activity was between 0.960-27.694 and 0.461-26.322 µmol/min/mg protein, glucosinolate content was between 0-2291.8 and 0-248.5 µg/g fresh weight for sinigrin and gluconastrutiin, respectively. The possible specificity of plants to different glucosinolates was also shown. Allyl isothiocyanate release rate was different in different vegetables (73.13 - 102.13%). The method could also be used for quantification of allyl isothiocyanate from food products. CONCLUSIONS: The presented capillary electrophoresis method requires a minimal amount of sample and contains only a few sample preparation steps, and can be used in several applications (glucosinolate determination, myrosinase activity measurement, isothiocyanate release estimation). Copyright © 2016 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Cromatografia Capilar Eletrocinética Micelar/métodos
Eletroforese Capilar/métodos
Glucosinolatos/análise
Glicosídeo Hidrolases/análise
Isotiocianatos/análise
Extratos Vegetais/química
Verduras/química
[Mh] Termos MeSH secundário: Armoracia/química
Armoracia/enzimologia
Brassica/química
Brassica/enzimologia
Glucosinolatos/metabolismo
Glicosídeo Hidrolases/metabolismo
Isotiocianatos/metabolismo
Estrutura Molecular
Nasturtium/química
Nasturtium/enzimologia
Raphanus/química
Raphanus/enzimologia
Sensibilidade e Especificidade
Fatores de Tempo
Verduras/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucosinolates); 0 (Isothiocyanates); 0 (Plant Extracts); 50UM64RMBJ (sinigrin); BN34FX42G3 (allyl isothiocyanate); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.147 (thioglucosidase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160618
[St] Status:MEDLINE
[do] DOI:10.1002/pca.2615


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[PMID]:27206485
[Au] Autor:Voutsina N; Payne AC; Hancock RD; Clarkson GJ; Rothwell SD; Chapman MA; Taylor G
[Ad] Endereço:Centre for Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
[Ti] Título:Characterization of the watercress (Nasturtium officinale R. Br.; Brassicaceae) transcriptome using RNASeq and identification of candidate genes for important phytonutrient traits linked to human health.
[So] Source:BMC Genomics;17:378, 2016 05 20.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Consuming watercress is thought to provide health benefits as a consequence of its phytonutrient composition. However, for watercress there are currently limited genetic resources underpinning breeding efforts for either yield or phytonutritional traits. In this paper, we use RNASeq data from twelve watercress accessions to characterize the transcriptome, perform candidate gene mining and conduct differential expression analysis for two key phytonutritional traits: antioxidant (AO) capacity and glucosinolate (GLS) content. RESULTS: The watercress transcriptome was assembled to 80,800 transcripts (48,732 unigenes); 71 % of which were annotated based on orthology to Arabidopsis. Differential expression analysis comparing watercress accessions with 'high' and 'low' AO and GLS resulted in 145 and 94 differentially expressed loci for AO capacity and GLS respectively. Differentially expressed loci between high and low AO watercress were significantly enriched for genes involved in plant defence and response to stimuli, in line with the observation that AO are involved in plant stress-response. Differential expression between the high and low GLS watercress identified links to GLS regulation and also novel transcripts warranting further investigation. Additionally, we successfully identified watercress orthologs for Arabidopsis phenylpropanoid, GLS and shikimate biosynthesis pathway genes, and compiled a catalogue of polymorphic markers for future applications. CONCLUSIONS: Our work describes the first transcriptome of watercress and establishes the foundation for further molecular study by providing valuable resources, including sequence data, annotated transcripts, candidate genes and markers.
[Mh] Termos MeSH primário: Genes de Plantas
Sequenciamento de Nucleotídeos em Larga Escala
Nasturtium/genética
Característica Quantitativa Herdável
Transcriptoma
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Biologia Computacional/métodos
Perfilação da Expressão Gênica
Glucosinolatos/metabolismo
Seres Humanos
Anotação de Sequência Molecular
Nasturtium/química
Fenótipo
Filogenia
Compostos Fitoquímicos
Plantas Comestíveis/química
Plantas Comestíveis/genética
Polimorfismo Genético
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Glucosinolates); 0 (Phytochemicals)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-2704-4


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[PMID]:27164268
[Au] Autor:Cordeiro C; Favas PJC; Pratas J; Sarkar SK; Venkatachalam P
[Ad] Endereço:MARE, Marine and Environmental Sciences Centre, Faculty of Sciences and Technology, University of Coimbra, 3004-517, Coimbra, Portugal.
[Ti] Título:Uranium accumulation in aquatic macrophytes in an uraniferous region: Relevance to natural attenuation.
[So] Source:Chemosphere;156:76-87, 2016 Aug.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytoremediation potential of uranium (U) was investigated by submerged, free-floating and rooted emergent native aquatic macrophytes inhabiting along the streams of Horta da Vilariça, a uraniferous geochemical region of NE Portugal. The work has been undertaken with the following objectives: (i) to relate the U concentrations in water-sediment-plant system; and (ii) to identify the potentialities of aquatic plants to remediate U-contaminated waters based on accumulation pattern. A total of 25 plant species culminating 233 samples was collected from 15 study points along with surface water and contiguous sediments. Concentrations of U showed wide range of variations both in waters (0.61-5.56 µg L(-1), mean value 1.98 µg L(-1)) and sediments (124-23,910 µg kg(-1), mean value 3929 µg kg(-1)) and this is also reflected in plant species examined. The plant species exhibited the ability to accumulate U several orders of magnitude higher than the surrounding water. Maximum U concentrations was recorded in the bryophyte Scorpiurium deflexifolium (49,639 µg kg(-1)) followed by Fontinalis antipyretica (35,771 µg kg(-1)), shoots of Rorippa sylvestris (33,837 µg kg(-1)), roots of Oenanthe crocata (17,807 µg kg(-1)) as well as in Nasturtium officinale (10,995 µg kg(-1)). Scorpiurium deflexifolium displayed a high bioconcentration factor (BF) of ∼2.5 × 10(4) (mean value). The species Fontinalis antipyretica, Nasturtium officinale (roots) and Rorippa sylvestris (shoots) exhibited the mean BFs of 1.7 × 10(4), 5 × 10(3) and 4.8 × 10(3) respectively. Maximum translocation factor (TF) was very much pronounced in the rooted perennial herb Rorippa sylvestris showing extreme ability to transport U for the shoots and seems to be promising candidate to be used as bioindicator species.
[Mh] Termos MeSH primário: Urânio/metabolismo
Poluentes Radioativos da Água/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Briófitas/metabolismo
Bryopsida/metabolismo
Monitoramento Ambiental
Nasturtium/metabolismo
Oenanthe/metabolismo
Raízes de Plantas/metabolismo
Brotos de Planta/metabolismo
Portugal
Rios/química
Rorippa/metabolismo
Urânio/análise
Poluentes Radioativos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Radioactive); 4OC371KSTK (Uranium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160511
[St] Status:MEDLINE


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[PMID]:27161402
[Au] Autor:Tran HT; Márton MR; Herz C; Maul R; Baldermann S; Schreiner M; Lamy E
[Ad] Endereço:University of Freiburg - Medical Center, Institute of Environmental Health Sciences, Molecular Preventive Medicine, Breisacher Strasse 115b, 79106 Freiburg, Germany; University of Freiburg - Medical Center, Institute for Prevention and Cancer Epidemiology, Molecular Preventive Medicine, Elsässerstr.
[Ti] Título:Nasturtium (Indian cress, Tropaeolum majus nanum) dually blocks the COX and LOX pathway in primary human immune cells.
[So] Source:Phytomedicine;23(6):611-20, 2016 Jun 01.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Nasturtium (Indian cress, Tropaeolum majus) is known for its pharmacological value in the treatment of bacterial infections of the upper air tract and urinary bladder. However, scientific data on the anti-inflammatory potency in human-derived cells is missing. PURPOSE: The aim of this study was to investigate the potential of nasturtium to inhibit the lipopolysaccharide (LPS) induced inflammatory response in primary human cells of the immune system. STUDY DESIGN: The anti-inflammatory activities of nasturtium and its fractions were evaluated via regulation of arachidonic acid (AA) pathway and MAPK kinase cascade. Fraction H4 which was responsible for the anti-inflammatory effects was further characterized. METHODS: Human peripheral blood mononuclear cells (PBMC) were either treated with plant extracts or fractions thereof, stimulated with LPS and/or N-formyl-methionyl-leucyl-phenylalanine (fMLP) and analysed for COX and LOX, release of prostaglandin PGE2, leukotriene LTB4, TNF-alpha and ERK signaling pathway activation. The plant extracts were separated into four fractions by HPLC; fraction H4 was subjected to UHPLC-ToF/MS analysis to identify potential bioactive compounds. RESULTS: We found that aqueous extracts of nasturtium did exert strong concentration dependent suppression of LPS-triggered TNF-alpha release and COX pathway signaling, including PGE2 synthesis. Whereas COX-1 protein expression was not impacted, LPS-triggered COX-2 protein expression was concentration dependently blocked by the plant extract but not COX-2 enzyme activity. These findings suggest a mechanism of action for the plant extract which is different from non-steroidal anti-inflammatory drugs (NSAIDs). Moreover, the plant extract blocked leukotriene LTB4 release, the major end product of the 5-LOX pathway from PBMC. Down-regulation of ERK1/2 and c-Jun activation preceded COX-2 suppression upon plant extract treatment in the presence of LPS. Using HPLC separation of the aqueous extract followed by metabolomic analysis we could limit the number of relevant bioactive compounds in the extract to about 50. CONCLUSIONS: This study provides a rationale for the anti-inflammatory efficacy of nasturtium observed in man and gives first insight into the underlying molecular mechanisms.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Ciclo-Oxigenase 2/metabolismo
Inibidores Enzimáticos/farmacologia
Inflamação/induzido quimicamente
Inflamação/tratamento farmacológico
Lipopolissacarídeos/efeitos adversos
Lipoxigenase/metabolismo
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Masculino
Nasturtium/química
Extratos Vegetais/farmacologia
Tropaeolum/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Enzyme Inhibitors); 0 (Lipopolysaccharides); 0 (Plant Extracts); EC 1.13.11.12 (Lipoxygenase); EC 1.14.99.1 (Cyclooxygenase 2)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160511
[St] Status:MEDLINE


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[PMID]:27052411
[Au] Autor:Pedras MS; To QH
[Ad] Endereço:Department of Chemistry, University of Saskatchewan, 10 Science Place, Saskatoon SK S7N 5C9, Canada. s.pedras@usask.ca.
[Ti] Título:Unveiling the first indole-fused thiazepine: structure, synthesis and biosynthesis of cyclonasturlexin, a remarkable cruciferous phytoalexin.
[So] Source:Chem Commun (Camb);52(34):5880-3, 2016 Apr 30.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:As a mechanism of defense against pathogens and other types of stress, watercress plants produce a variety of elicited chemical defenses generally known as phytoalexins. Herein the chemical structure, synthesis, biosynthesis and antifungal activity of cyclonasturlexin, the most intriguing indolyl phytoalexin isolated to date, are reported.
[Mh] Termos MeSH primário: Indóis/farmacologia
Nasturtium/metabolismo
Tiazepinas/farmacologia
[Mh] Termos MeSH secundário: Alternaria/efeitos dos fármacos
Ciclização
Fungicidas Industriais/síntese química
Fungicidas Industriais/metabolismo
Fungicidas Industriais/farmacologia
Indóis/síntese química
Indóis/metabolismo
Tiazepinas/síntese química
Tiazepinas/metabolismo
Tiocarbamatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungicides, Industrial); 0 (Indoles); 0 (Thiazepines); 0 (Thiocarbamates); 0 (cyclonasturlexin); 105748-59-2 (brassinin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160408
[St] Status:MEDLINE
[do] DOI:10.1039/c6cc02108e



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