Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.157.711 [Categoria DeCS]
Referências encontradas : 801 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 81 ir para página                         

  1 / 801 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29251899
[Au] Autor:Kaur N; Jhanji S
[Ti] Título:Effect of soil cadmium on growth, photosynthesis and quality of Raphanus sativus and Lactuca sativa.
[So] Source:J Environ Biol;37(5):993-7, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Cadmium (Cd) raises serious concerns as its accumulation in the plant not only affect the growth and quality of plant but also threaten the health of consumers. In this research, two vegetables, i.e., radish (Raphanus sativus L.) and lettuce (Lactuca sativa L), were planted in pots having soil treated with Cd as Cd (NO3)2 at different doses (25, 50, 100 and 200 mg Cd kg-1 soil ) to investigate the influence of cadmium on their growth, photosynthetic attributes and quality. Cadmium retarded plant growth as dry weight of radish roots decline by 87% and leaves by 83% following 200 mg Cd kg-1 soil application and the corresponding values for lettuce were 64 and 69% respectively. Significant reductions in various photosynthetic parameters viz., leaf area per plant, total chlorophyll, Chl a and Chl b content were also recorded with Cd applications. The Cd treatments resulted in loss of membrane integrity as revealed by significant increase in electrolyte leakage in leaves of both vegetables. There was significant increase in Cd accumulation in radish and lettuce with all applications but no visual symptoms of Cd toxicity were noticed with 25 and 50 mg Cd kg-1 soil application except for yield differences, illustrating that Cd accumulate in this crop without visual evidence of its presence. However, toxicity symptoms in the form of interveinal chlorosis of the leaf lamina, followed by necrosis and leaf rolling, were clearly evident with 100 and 200 mg Cd kg-1 soil application. Apparently, Cd causes harm due to its phytotoxic effects and high accumulation in edible parts of radish and lettuce without any visible symptoms that constitutes a substantial hazard to human health.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Alface/efeitos dos fármacos
Fotossíntese/efeitos dos fármacos
Raphanus/efeitos dos fármacos
Poluentes do Solo/química
Solo/química
[Mh] Termos MeSH secundário: Cádmio/química
Clorofila/metabolismo
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 0 (Soil Pollutants); 00BH33GNGH (Cadmium); 1406-65-1 (Chlorophyll)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  2 / 801 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28992475
[Au] Autor:Akhtar MJ; Ullah S; Ahmad I; Rauf A; Nadeem SM; Khan MY; Hussain S; Bulgariu L
[Ad] Endereço:Institute of Soil & Environmental Sciences, University of Agriculture Faisalabad, 38040, Pakistan.
[Ti] Título:Nickel phytoextraction through bacterial inoculation in Raphanus sativus.
[So] Source:Chemosphere;190:234-242, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A pot experiment was conducted to evaluate the potential of two plant growth promoting rhizobacteria (PGPR) viz. Bacillus sp. CIK-516 and Stenotrophomonas sp. CIK-517Y for improving the growth and Ni uptake of radish (Raphanus sativus) in the presence of four different levels of Ni contamination (0, 50, 100, 150 mg Ni kg soil). Plant growth, dry biomass, chlorophyll and nitrogen contents were significantly reduced by the exogenous application of Ni, however, bacterial inoculation diluted the negative impacts of Ni stress on radish by improving these parameters. PGPR strain CIK-516 increased root length (9-27%), shoot length (8-27%), root dry biomass (2-32%), shoot dry biomass (9-51%), root girth (6-48%), total chlorophyll (4-38%) and shoot nitrogen contents (11-15%) in Ni contaminated and non-contaminated soils. Positive regulation of chlorophyll and nitrogen contents by the inoculated plants shows plant tolerance mechanism of Ni stress. Bacterial strain (CIK-516) exhibited indole acetic acid and 1-amino-cyclopropane-1-carboxylate deaminase potentials which would have helped radish plant to stabilize in Ni contaminated soil and thereby increased Ni uptake (24-257 in shoot and 58-609 in root mg kg dry biomass) and facilitated accumulation in radish (bioaccumulation factor = 0.6-1.7) depending upon soil Ni contamination. Based on the findings of this study, it might be suggested that inoculation with bacterial strain CIK-516 could be an efficient tool for enhanced Ni phytoextraction in radish.
[Mh] Termos MeSH primário: Recuperação e Remediação Ambiental/métodos
Níquel/isolamento & purificação
Raphanus/microbiologia
Poluentes do Solo/isolamento & purificação
[Mh] Termos MeSH secundário: Inoculantes Agrícolas
Bacillus/metabolismo
Clorofila/análise
Níquel/farmacologia
Nitrogênio/análise
Desenvolvimento Vegetal/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Raphanus/crescimento & desenvolvimento
Rhizobiaceae/metabolismo
Poluentes do Solo/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 1406-65-1 (Chlorophyll); 7OV03QG267 (Nickel); N762921K75 (Nitrogen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE


  3 / 801 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29247643
[Au] Autor:Wang H; Wang F; Wu S; Liu Z; Li T; Mao L; Zhang J; Li C; Liu C; Yang Y
[Ad] Endereço:Center for Molecular Medicine (CMM), School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China.
[Ti] Título:Traditional herbal medicine-derived sulforaphene promotes mitophagic cell death in lymphoma cells through CRM1-mediated p62/SQSTM1 accumulation and AMPK activation.
[So] Source:Chem Biol Interact;281:11-23, 2018 Feb 01.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Sulforaphene (LFS-01) is the major chemical constituent of Raphanus sativus, a medicinal herb used for over a thousand years in traditional Chinese medicine. Here we identified that LFS-01 can selectively eradicate lymphoma cells while sparing normal lymphocytes by triggering concomitant mitophagy and apoptosis. We demonstrated that LFS-01 can retain Nrf2 in the nucleus by covalently modulating CRM1 and consequently upregulate p62/SQSTM1, an essential structural component of the autophagosomes during mitophagic process. We found that LFS-01 treatment also stimulated AMPK and thereby inhibited the mTOR pathway. On the contrary, we revealed that AMPK inhibition can severely impair the LFS-01-mediated mitophagy. Transcriptomic studies confirmed that 15 autophagy-associated genes such as p62/SQSTM1, VCP and BCL2 were differentially expressed after LFS-01 treatment. Furthermore, protein interactome network analysis revealed that the events of apoptosis and the assembly of autophagy vacuole were significant upon LFS-01 exposure. Lastly, we found that LFS-01 exhibited strong efficacy in xenograft mouse model yet with the lack of apparent toxicity to animals. We concluded that LFS-01 triggered mitophagic cell death via CRM1-mediated p62 overexpression and AMPK activation. Our findings provide new insights into the mechanism of action for LFS-01 and highlight its potential applications in treating major human diseases.
[Mh] Termos MeSH primário: Proteínas Quinases Ativadas por AMP/metabolismo
Apoptose/efeitos dos fármacos
Medicamentos de Ervas Chinesas/química
Isotiocianatos/farmacologia
Carioferinas/metabolismo
Degradação Mitocondrial/efeitos dos fármacos
Receptores Citoplasmáticos e Nucleares/metabolismo
Proteína Sequestossoma-1/metabolismo
[Mh] Termos MeSH secundário: Animais
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Seres Humanos
Isotiocianatos/química
Isotiocianatos/uso terapêutico
Leucócitos Mononucleares/citologia
Leucócitos Mononucleares/efeitos dos fármacos
Leucócitos Mononucleares/metabolismo
Linfoma/tratamento farmacológico
Linfoma/metabolismo
Linfoma/patologia
Camundongos
Camundongos Endogâmicos BALB C
Neoplasias/tratamento farmacológico
Neoplasias/metabolismo
Neoplasias/patologia
Estrutura Terciária de Proteína
Raphanus/química
Raphanus/metabolismo
Transdução de Sinais/efeitos dos fármacos
Regulação para Cima/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Isothiocyanates); 0 (Karyopherins); 0 (Receptors, Cytoplasmic and Nuclear); 0 (SQSTM1 protein, human); 0 (Sequestosome-1 Protein); 0 (exportin 1 protein); 0 (sulphoraphene); EC 2.7.11.31 (AMP-Activated Protein Kinases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE


  4 / 801 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28941906
[Au] Autor:Yang Z; Chambers H; DiCaprio E; Gao G; Li J
[Ad] Endereço:Department of Biology, College of Life Science, Huzhou University, Huzhou, Zhejiang, China.
[Ti] Título:Internalization and dissemination of human norovirus and Tulane virus in fresh produce is plant dependent.
[So] Source:Food Microbiol;69:25-32, 2018 Feb.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human norovirus (NoV) is a leading cause of fresh produce associated outbreaks. Previous research indicates that the roots of growing leafy greens and berries internalize human NoV. However the effect of plant type and inoculum level on internalization rates has not been directly compared. In this study we compared the internalization and dissemination rates of human NoV and its surrogate, Tulane virus (TV) in green onion, radishes, and Romaine lettuce. We also evaluated the effect inoculum level and plant growth matrix on the rate of viral internalization. In the hydroponic growth system, we detected internalization and dissemination of human NoV RNA in green onions. In hydroponically growing green onions inoculated with high titer TV, we found higher rates of internalization and dissemination compared to green onions inoculated with low titer TV. In soil growth systems, no infectious TV was detected in either green onion or radishes. However, in Romaine lettuce plants grown in soil approximately 4 log PFU/g was recovered from all tissues on day 14 p.i. Overall, we found that the type of plant, growth matrix, and the inoculum level influences the internalization and dissemination of human NoV and TV.
[Mh] Termos MeSH primário: Caliciviridae/fisiologia
Contaminação de Alimentos/análise
Alface/virologia
Norovirus/fisiologia
Cebolas/virologia
Raphanus/virologia
Verduras/virologia
Internalização do Vírus
[Mh] Termos MeSH secundário: Caliciviridae/genética
Caliciviridae/isolamento & purificação
Seres Humanos
Alface/crescimento & desenvolvimento
Norovirus/genética
Norovirus/isolamento & purificação
Cebolas/crescimento & desenvolvimento
Raphanus/crescimento & desenvolvimento
Microbiologia do Solo
Verduras/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE


  5 / 801 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28539223
[Au] Autor:Cardenia V; Vivarelli F; Cirillo S; Paolini M; Rodriguez-Estrada MT; Canistro D
[Ad] Endereço:Interdepartmental Centre for Agri-Food Industrial Research, Alma Mater Studiorum - University of Bologna, Cesena, Italy.
[Ti] Título:Dietary effects of Raphanus sativus cv Sango on lipid and oxysterols accumulation in rat brain: A lipidomic study on a non-genetic obesity model.
[So] Source:Chem Phys Lipids;207(Pt B):206-213, 2017 Oct.
[Is] ISSN:1873-2941
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:The present study aimed to evaluate the impact of Raphanus sativus cv Sango sprout juice (SSJ) administration (75mg/kg b.w. SSJ/day) on the brain lipidomic profile (fatty acid, sterols, cholesterol oxidation) of rats (non-genetic model) subjected to a high-fat (34% crude fat) dietary regimen. The SSJ did not affect the lipid infiltration (7.7-9.3%) and the fatty acid composition of the rat brain, which was mainly composed by unsaturated fatty acids (∼58%); however, the high-fat diet regimen significantly halved linoleic acid (LA). The high-fat diet also decreased (21.13mg/g) the level of brain cholesterol with respect to the regular diet (4.5% crude fat) (23.83mg/g); however, when the diet was shifted from high-fat to a regular regimen with or without SSJ supplementation, the levels of cholesterol significantly (p <0.05) increased up to 30.46mg/g of brain. The main oxysterols were 24(S)-hydroxycholesterol (24(S)-HC) and ß-epoxycholesterol (ß-EC). The high-fat diet led to the highest cholesterol oxidation (63.1µg/g), increasing 27-hydroxycholesterol (27-HC) infiltration (0.24µg/g rat brain) through the blood-brain barrier (BBB) compared to the regular diet (0.13µg/g rat brain). On the other hand, when the diet was switched from high-fat to a regular regimen with SSJ supplementation, a significant reduction of 27-HC in the rat brain was found. Although 24-HC did not significantly change (p=0.054), an increasing trend was observed when high-fat diet was supplied. The principal component analysis (PCA) revealed that SSJ was more active in counteracting cholesterol oxidation when supplied with the high-fat diet, due to inverse correlation with 24(S)-HC and 27-HC; however, further studies are needed to better understand which is the relationship between LA and cholesterol homeostasis in rat brain.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Dieta Hiperlipídica/efeitos adversos
Modelos Animais de Doenças
Lipídeos/análise
Obesidade/prevenção & controle
Oxisteróis/metabolismo
Raphanus/química
[Mh] Termos MeSH secundário: Animais
Colesterol/metabolismo
Sucos de Frutas e Vegetais
Ácido Linoleico/metabolismo
Masculino
Obesidade/metabolismo
Oxisteróis/análise
Análise de Componente Principal
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipids); 0 (Oxysterols); 97C5T2UQ7J (Cholesterol); 9KJL21T0QJ (Linoleic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE


  6 / 801 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28537731
[Au] Autor:Pawlowska B; Telesinski A; Platkowski M; Strek M; Snioszek M; Biczak R
[Ad] Endereço:The Faculty of Mathematics and Natural Sciences, Jan Dlugosz University in Czestochowa , 13/15 Armii Krajowej Av., 42-200 Czestochowa, Poland.
[Ti] Título:Reaction of Spring Barley and Common Radish on the Introduction of Ionic Liquids Containing Asymmetric Cations to the Soil.
[So] Source:J Agric Food Chem;65(23):4562-4571, 2017 Jun 14.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The harmful effect of ionic liquids (ILs) on the environment is one of the important elements of scientific research conducted around the world. This study presents the effect of ionic liquids, containing the asymmetric cations benzyltrimethylammonium chloride [BenzTMA][Cl] and benzyltriethylammonium chloride [BenzTEA][Cl], on physiological and biochemical changes in common radish plants and spring barley seedlings. The examined ILs demonstrated low toxicity to higher plants. The compound that exhibited higher phytotoxicity to these plant species was [BenzTMA][Cl], whereas the plant that was more resistant to such ILs was common radish. Both the ionic liquids, particularly at higher concentrations, led to changes in the metabolism of plants, which resulted in a decrease of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids content. The observed changes were positively correlated with increasing concentrations of the examined ILs in the soil. In the case of spring barley, a decrease in the fresh weight and an increase in the dry weight of the seedlings were also observed. The evidence of oxidative stress occurrence in spring barley was observed due to the accumulation of malondialdehyde and free proline, as well as due to an increase in the activity of catalase and peroxidase. The changes in these biomarkers indicating oxidative stress occurrence in common radish plants were much lower. An increase in the content of chloride ions was observed in both the plants.
[Mh] Termos MeSH primário: Hordeum/efeitos dos fármacos
Líquidos Iônicos/farmacologia
Raphanus/efeitos dos fármacos
Poluentes do Solo/farmacologia
[Mh] Termos MeSH secundário: Carotenoides/metabolismo
Cátions/metabolismo
Clorofila/análogos & derivados
Clorofila/metabolismo
Hordeum/crescimento & desenvolvimento
Hordeum/metabolismo
Malondialdeído/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Raphanus/crescimento & desenvolvimento
Raphanus/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Ionic Liquids); 0 (Soil Pollutants); 1406-65-1 (Chlorophyll); 22309-13-3 (chlorophyll a'); 36-88-4 (Carotenoids); 4Y8F71G49Q (Malondialdehyde); 5712ZB110R (chlorophyll b)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00912


  7 / 801 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28533439
[Au] Autor:Adamczyk-Chauvat K; Delaunay S; Vannier A; François C; Thomas G; Eber F; Lodé M; Gilet M; Huteau V; Morice J; Nègre S; Falentin C; Coriton O; Darmency H; Alrustom B; Jenczewski E; Rousseau-Gueutin M; Chèvre AM
[Ad] Endereço:MaIAGE, INRA, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
[Ti] Título:Gene Introgression in Weeds Depends on Initial Gene Location in the Crop: - Model.
[So] Source:Genetics;206(3):1361-1372, 2017 Jul.
[Is] ISSN:1943-2631
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effect of gene location within a crop genome on its transfer to a weed genome remains an open question for gene flow assessment. To elucidate this question, we analyzed advanced generations of intergeneric hybrids, derived from an initial pollination of known oilseed rape varieties ( , AACC, 2 = 38) by a local population of wild radish ( , RrRr, 2 = 18). After five generations of recurrent pollination, 307 G5 plants with a chromosome number similar to wild radish were genotyped using 105 specific markers well distributed along the chromosomes. They revealed that 49.8% of G5 plants carried at least one genomic region. According to the frequency of markers (0-28%), four classes were defined: Class 1 (near zero frequency), with 75 markers covering ∼70% of oilseed rape genome; Class 2 (low frequency), with 20 markers located on 11 genomic regions; Class 3 (high frequency), with eight markers on three genomic regions; and Class 4 (higher frequency), with two adjacent markers detected on A10. Therefore, some regions of the oilseed rape genome are more prone than others to be introgressed into wild radish. Inheritance and growth of plant progeny revealed that genomic regions of oilseed rape could be stably introduced into wild radish and variably impact the plant fitness (plant height and seed number). Our results pinpoint that novel technologies enabling the targeted insertion of transgenes should select genomic regions that are less likely to be introgressed into the weed genome, thereby reducing gene flow.
[Mh] Termos MeSH primário: Brassica/genética
Fluxo Gênico
Genes de Plantas
Raphanus/genética
[Mh] Termos MeSH secundário: Aptidão Genética
Hibridização Genética
Plantas Daninhas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.1534/genetics.117.201715


  8 / 801 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28530612
[Au] Autor:Lee JG; Lim S; Kim J; Lee EJ
[Ad] Endereço:Department of Plant Science, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
[Ti] Título:The mechanism of deterioration of the glucosinolate-myrosynase system in radish roots during cold storage after harvest.
[So] Source:Food Chem;233:60-68, 2017 Oct 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The hydrolysis of glucosinolates (GSLs) by myrosinase yields varieties of degradation products including isothiocyanates (ITCs). This process is controlled by the glucosinolate-myrosinase (G-M) system. The major ITCs in radish roots are raphasatin and sulforaphene (SFE), and the levels of these compounds decrease during storage after harvest. We investigated the G-M system to understand the mechanism behind the decrease in the ITCs in radish roots. Six varieties of radish roots were stored for 8weeks at 0-1.5°C. The concentrations of GSLs (glucoraphasatin and glucoraphenin) were maintained at harvest levels without significant changes during the storage period. However, SFE concentration and myrosinase activity remarkably decreased for 8weeks. Pearson correlation analysis between ITCs, GSLs, and myrosinase activity showed that a decrease of SFE during storage had a positive correlation with a decrease in myrosinase activity, which resulted from a decrease of ascorbic acid but also a decrease of myrosinase activity-related gene expressions.
[Mh] Termos MeSH primário: Raphanus
[Mh] Termos MeSH secundário: Temperatura Baixa
Glucosinolatos
Glicosídeo Hidrolases
Hidrólise
Isotiocianatos
Raízes de Plantas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucosinolates); 0 (Isothiocyanates); EC 3.2.1.- (Glycoside Hydrolases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE


  9 / 801 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28515021
[Au] Autor:Pawlik A; Wala M; Hac A; Felczykowska A; Herman-Antosiewicz A
[Ad] Endereço:Department of Medical Biology and Genetics, University of Gdansk, Wita Stwosza 59, Gdansk 80-308, Poland.
[Ti] Título:Sulforaphene, an isothiocyanate present in radish plants, inhibits proliferation of human breast cancer cells.
[So] Source:Phytomedicine;29:1-10, 2017 Jun 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Isothiocyanates derived from the Brassicaceae plants possess chemopreventive and anticancer activities. One of them is sulforaphene (SF), which is abundant in Rhapanus sativus seeds. The underlying mechanism of its anticancer activity is still underexplored. PURPOSE: SF properties make it an interesting candidate for cancer prevention and therapy. Thus, it is crucial to characterize the mechanism of its activity. STUDY DESIGN: We investigated the mechanism of antiproliferative activity of SF in breast cancer cells differing in growth factor receptors status and lacking functional p53. METHODS: Viability of SKBR-3 and MDA-MB-231 breast cancer cells treated with SF was determined by SRB and clonogenic assays. Cell cycle, cell death and oxidative stress were analyzed by flow cytometry or microscopy. The levels of apoptosis and autophagy markers were assessed by immunoblotting. RESULTS: SF efficiently decreased the viability of breast cancer cells, while normal cells (MCF10A) were less sensitive to the analyzed isothiocyanate. SF induced G2/M cell cycle arrest, as well as disturbed cytoskeletal organization and reduced clonogenic potential of the cancer cells. SF induced apoptosis in a concentration-dependent manner which was associated with the oxidative stress, mitochondria dysfunction, increased Bax:Bcl2 ratio and ADRP levels. SF also potentiated autophagy which played a cytoprotective role. CONCLUSIONS: SF exhibits cytotoxic activity against breast cancer cells even at relatively low concentrations (5-10µM). This is associated with induction of the cell cycle arrest and apoptosis. SF might be considered as a potent anticancer agent.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Neoplasias da Mama/tratamento farmacológico
Isotiocianatos/farmacologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Neoplasias da Mama/patologia
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Feminino
Seres Humanos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Raphanus/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Isothiocyanates); 0 (sulphoraphene)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE


  10 / 801 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28498850
[Au] Autor:Liu C; Wang S; Xu W; Liu X
[Ad] Endereço:Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Shandong Key Laboratory of Greenhouse Vegetable Biology, Shandong Branch of National Vegetable Improvement Center, Jinan, Shandong, People's Republic of China.
[Ti] Título:Genome-wide transcriptome profiling of radish (Raphanus sativus L.) in response to vernalization.
[So] Source:PLoS One;12(5):e0177594, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vernalization is a key process for premature bolting. Although many studies on vernalization have been reported, the molecular mechanism of vernalization is still largely unknown in radish. In this study, we sequenced the transcriptomes of radish seedlings at three different time points during vernalization. More than 36 million clean reads were generated for each sample and the portions mapped to the reference genome were all above 67.0%. Our results show that the differentially expressed genes (DEGs) between room temperature and the early stage of vernalization (4,845) are the most in all treatments pairs. A series of vernalization related genes, including two FLOWERING LOCUS C (FLC) genes, were screened according to the annotations. A total of 775 genes were also filtered as the vernalization related candidates based on their expression profiles. Cold stress responsive genes were also analyzed to further confirm the sequencing result. Several key genes in vernalization or cold stress response were validated by quantitative RT-PCR (RT-qPCR). This study identified a number of genes that may be involved in vernalization, which are useful for other functional genomics research in radish.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica/métodos
Raphanus/genética
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas/genética
Proteínas de Plantas/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Temperatura Ambiente
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177594



página 1 de 81 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde