Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.157.744 [Categoria DeCS]
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  1 / 22 MEDLINE  
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[PMID]:27164268
[Au] Autor:Cordeiro C; Favas PJC; Pratas J; Sarkar SK; Venkatachalam P
[Ad] Endereço:MARE, Marine and Environmental Sciences Centre, Faculty of Sciences and Technology, University of Coimbra, 3004-517, Coimbra, Portugal.
[Ti] Título:Uranium accumulation in aquatic macrophytes in an uraniferous region: Relevance to natural attenuation.
[So] Source:Chemosphere;156:76-87, 2016 Aug.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytoremediation potential of uranium (U) was investigated by submerged, free-floating and rooted emergent native aquatic macrophytes inhabiting along the streams of Horta da Vilariça, a uraniferous geochemical region of NE Portugal. The work has been undertaken with the following objectives: (i) to relate the U concentrations in water-sediment-plant system; and (ii) to identify the potentialities of aquatic plants to remediate U-contaminated waters based on accumulation pattern. A total of 25 plant species culminating 233 samples was collected from 15 study points along with surface water and contiguous sediments. Concentrations of U showed wide range of variations both in waters (0.61-5.56 µg L(-1), mean value 1.98 µg L(-1)) and sediments (124-23,910 µg kg(-1), mean value 3929 µg kg(-1)) and this is also reflected in plant species examined. The plant species exhibited the ability to accumulate U several orders of magnitude higher than the surrounding water. Maximum U concentrations was recorded in the bryophyte Scorpiurium deflexifolium (49,639 µg kg(-1)) followed by Fontinalis antipyretica (35,771 µg kg(-1)), shoots of Rorippa sylvestris (33,837 µg kg(-1)), roots of Oenanthe crocata (17,807 µg kg(-1)) as well as in Nasturtium officinale (10,995 µg kg(-1)). Scorpiurium deflexifolium displayed a high bioconcentration factor (BF) of ∼2.5 × 10(4) (mean value). The species Fontinalis antipyretica, Nasturtium officinale (roots) and Rorippa sylvestris (shoots) exhibited the mean BFs of 1.7 × 10(4), 5 × 10(3) and 4.8 × 10(3) respectively. Maximum translocation factor (TF) was very much pronounced in the rooted perennial herb Rorippa sylvestris showing extreme ability to transport U for the shoots and seems to be promising candidate to be used as bioindicator species.
[Mh] Termos MeSH primário: Urânio/metabolismo
Poluentes Radioativos da Água/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Briófitas/metabolismo
Bryopsida/metabolismo
Monitoramento Ambiental
Nasturtium/metabolismo
Oenanthe/metabolismo
Raízes de Plantas/metabolismo
Brotos de Planta/metabolismo
Portugal
Rios/química
Rorippa/metabolismo
Urânio/análise
Poluentes Radioativos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Radioactive); 4OC371KSTK (Uranium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160511
[St] Status:MEDLINE


  2 / 22 MEDLINE  
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[PMID]:26796345
[Au] Autor:Xu K; Chang Y; Zhang Y; Liu K; Zhang J; Wang W; Li Z; Wu J; Ma S; Xin Y; Li C; Zhou Q; Qiu H; Pi Y; Wang Y; Tan G; Li C
[Ad] Endereço:Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, People's Republic of China.
[Ti] Título:Rorippa indica Regeneration via Somatic Embryogenesis Involving Frog Egg-like Bodies Efficiently Induced by the Synergy of Salt and Drought Stresses.
[So] Source:Sci Rep;6:19811, 2016 Jan 22.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Frog egg-like bodies (FELBs), novel somatic embryogenesis (SE) structures first observed in Solanum nigrum, were induced in Rorippa indica. NaCl-mediated salt and mannitol-mimicked drought stresses induced FELBs in R. indica, which is very different from the induction by plant growth regulators (PGRs) under low light condition that was used in S. nigrum FELB induction. It demonstrated that NaCl or mannitol supplements alone could induce FELBs in R. indica, but with low induction rates, while the synergy of NaCl and mannitol significantly increased the FELB induction rates. For the combination of 5.0 g/L mannitol and 10.0 g/L NaCl the highest FELB induction rate (100%) was achieved. It suggests that the synergy of drought and salt stresses can replace PGRs to induce FELBs in R. indica. On medium supplemented with 1.0 mg/L gibberellic acid all the inoculated in vitro FELBs developed into multiple plantlets. Morphological and histological analyses confirmed the identity of FELBs induced in R. indica and revealed that FELBs originate from root cortex cells.
[Mh] Termos MeSH primário: Secas
Técnicas de Embriogênese Somática de Plantas/métodos
Regeneração/efeitos dos fármacos
Rorippa/fisiologia
Cloreto de Sódio/farmacologia
Estresse Fisiológico/efeitos dos fármacos
[Mh] Termos MeSH secundário: Ácido 2,4-Diclorofenoxiacético/farmacologia
Animais
Anuros
Secções Congeladas
Ácidos Indolacéticos/farmacologia
Luz
Manitol/farmacologia
Óvulo
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/fisiologia
Raízes de Plantas/efeitos da radiação
Regeneração/efeitos da radiação
Rorippa/efeitos dos fármacos
Rorippa/efeitos da radiação
Estresse Fisiológico/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Indoleacetic Acids); 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid); 3OWL53L36A (Mannitol); 451W47IQ8X (Sodium Chloride); 6U1S09C61L (indoleacetic acid)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160123
[St] Status:MEDLINE
[do] DOI:10.1038/srep19811


  3 / 22 MEDLINE  
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[PMID]:26569502
[Au] Autor:Amano R; Nakayama H; Morohoshi Y; Kawakatsu Y; Ferjani A; Kimura S
[Ad] Endereço:Department of Bioresource and Environmental Sciences, Kyoto Sangyo University, Kyoto-City, Kyoto, Japan.
[Ti] Título:A Decrease in Ambient Temperature Induces Post-Mitotic Enlargement of Palisade Cells in North American Lake Cress.
[So] Source:PLoS One;10(11):e0141247, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In order to maintain organs and structures at their appropriate sizes, multicellular organisms orchestrate cell proliferation and post-mitotic cell expansion during morphogenesis. Recent studies using Arabidopsis leaves have shown that compensation, which is defined as post-mitotic cell expansion induced by a decrease in the number of cells during lateral organ development, is one example of such orchestration. Some of the basic molecular mechanisms underlying compensation have been revealed by genetic and chimeric analyses. However, to date, compensation had been observed only in mutants, transgenics, and γ-ray-treated plants, and it was unclear whether it occurs in plants under natural conditions. Here, we illustrate that a shift in ambient temperature could induce compensation in Rorippa aquatica (Brassicaceae), a semi-aquatic plant found in North America. The results suggest that compensation is a universal phenomenon among angiosperms and that the mechanism underlying compensation is shared, in part, between Arabidopsis and R. aquatica.
[Mh] Termos MeSH primário: Arabidopsis/crescimento & desenvolvimento
Tamanho Celular
Mitose
Rorippa/crescimento & desenvolvimento
Temperatura Ambiente
[Mh] Termos MeSH secundário: Arabidopsis/citologia
Arabidopsis/genética
Comunicação Celular
Ciclo Celular
Proliferação Celular
Meio Ambiente
Regulação da Expressão Gênica de Plantas
Fenótipo
Filogenia
Folhas de Planta/citologia
Fenômenos Fisiológicos Vegetais
Rorippa/citologia
Rorippa/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0141247


  4 / 22 MEDLINE  
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[PMID]:26367499
[Au] Autor:Nakayama H; Kimura S
[Ad] Endereço:a Department of Plant Biology ; University of California Davis; One Shields Avenue , Davis , CA USA.
[Ti] Título:Leaves may function as temperature sensors in the heterophylly of Rorippa aquatica (Brassicaceae).
[So] Source:Plant Signal Behav;10(12):e1091909, 2015.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many plants show heterophylly, which is variation in leaf form within a plant owing to environmental change. The molecular mechanisms underlying heterophylly have recently been investigated in several plant species. However, little is known about how plants exhibiting heterophylly sense environmental cues. Here, we used Rorippa aquatica (Brassicaceae), which shows heterophylly, to investigate whether a single leaf can sense and transit changes in ambient temperature. The morphology of newly developed leaves after single-leaf warming treatment was significantly different from that of mock-treated control leaves, suggesting that leaves are sensing organs that mediate the responses to changes in ambient temperature in R. aquatica.
[Mh] Termos MeSH primário: Folhas de Planta/anatomia & histologia
Folhas de Planta/fisiologia
Rorippa/anatomia & histologia
Rorippa/fisiologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150915
[St] Status:MEDLINE
[do] DOI:10.1080/15592324.2015.1091909


  5 / 22 MEDLINE  
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[PMID]:25167813
[Au] Autor:Wei S; Ji D; Twardowska I; Li Y; Zhu J
[Ad] Endereço:Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, People's Republic of China, shuhewei@iae.ac.cn.
[Ti] Título:Effect of different nitrogenous nutrients on the cadmium hyperaccumulation efficiency of Rorippa globosa (Turcz.) Thell.
[So] Source:Environ Sci Pollut Res Int;22(3):1999-2007, 2015 Feb.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This experiment was used to explore whether the 11 nitrogenous nutrients affect the hyperaccumulation of Rorippa globosa (Turcz.) Thell. to Cd. Pot culture experiments using soil spiked with Cd as CdCl2·2.5H2O and 11 nitrogen-containing chemicals were conducted to determine the efficiency of the accumulation of Cd by R. globosa. Application of all 11 nitrogenous nutrients significantly (p < 0.05) enhanced Cd accumulation by R. globosa (Turcz.) Thell. Two major modes of Cd accumulation were observed: (i) through increase of biomass yield without reduction of Cd uptake and (ii) through increase of Cd uptake efficiency in parallel with increase of biomass yield. Bicarbonate > phosphate > chloride compounds of NH4 enhanced the biomass yield to the greatest extent, while oxalate > nitrate > chloride > and bicarbonate caused a significant increase of Cd uptake by R. globosa. Competition between N and Cd translocation caused either significant reduction of Cd translocation factor or decrease of biomass yield. Of studied nutrients, ammonium bicarbonate NH4HCO3 and ammonium chloride NH4Cl exerted the best joint effect of these two processes on the efficiency of R. globosa as a Cd hyperaccumulator. Application of these chemicals caused increase of Cd concentrations in roots of R. globosa by 35.1 and 41.1 %, and in shoots by 13.9 and 56.4 %, while biomasses of roots increased by 5.8- and 3.8-fold and in shoots by 7.4-fold, and 6.4-fold, respectively, compared to the control. As a result, accumulated load (µg pot(-1)) of Cd in roots increased by 8.2- and 5.8-fold and in shoots by 8.6- and 10.6-fold in both pots. Consequently, chemicals (NH4HCO3 and NH4Cl) that enhanced both Cd enrichment and biomass yield had the greatest effect on the bioaccumulation capacity of R. globosa.
[Mh] Termos MeSH primário: Cádmio/metabolismo
Rorippa/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Biomassa
Cádmio/química
Cloreto de Cádmio
Alimentos
Nitratos/farmacologia
Nitrogênio/farmacologia
Raízes de Plantas
Rorippa/química
Solo
Poluentes do Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Nitrates); 0 (Soil); 0 (Soil Pollutants); 00BH33GNGH (Cadmium); J6K4F9V3BA (Cadmium Chloride); N762921K75 (Nitrogen)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140830
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-014-3448-9


  6 / 22 MEDLINE  
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[PMID]:24893216
[Au] Autor:Lin LZ; Sun J; Chen P; Zhang RW; Fan XE; Li LW; Harnly JM
[Ad] Endereço:Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Building-161, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705, United States.
[Ti] Título:Profiling of glucosinolates and flavonoids in Rorippa indica (Linn.) Hiern. (Cruciferae) by UHPLC-PDA-ESI/HRMS(n).
[So] Source:J Agric Food Chem;62(26):6118-29, 2014 Jul 02.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An UHPLC-PDA-ESI/HRMS(n) profiling method was used to identify the glucosinolates and flavonoids of Rorippa indica (Cruciferae), a wild vegetable and Chinese herb used to treat cough, diarrhea, and rheumatoid arthritis. Thirty-three glucosinolates, more than 40 flavonol glycosides, and 18 other phenolic and common organic compounds were identified. The glucosinolates and polyphenols were separated by UHPLC. High-resolution deprotonated molecules provided high accuracy mass values that were used to determine formulas and provide putative identification of the glucosinolates and flavonoids. The fragments from multistage mass spectrometry were used to elucidate the structures. The concentrations of the main components were based on UV peak areas and molar relative response factors with a single calibration standard. This study found this plant to be a rich source for glucosinolates, containing 24 new glucosinolates, including 14 glucosylated glucosinolates that were previously unidentified.
[Mh] Termos MeSH primário: Flavonoides/análise
Alimento Funcional/análise
Glucosinolatos/análise
Rorippa/química
[Mh] Termos MeSH secundário: China
Cromatografia Líquida de Alta Pressão
Técnicas Eletroquímicas
Flavonoides/química
Alimento Funcional/economia
Glucosinolatos/química
Estrutura Molecular
Fotometria
Extratos Vegetais/química
Folhas de Planta/química
Plântulas/química
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Flavonoids); 0 (Glucosinolates); 0 (Plant Extracts)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140604
[St] Status:MEDLINE
[do] DOI:10.1021/jf405538d


  7 / 22 MEDLINE  
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[PMID]:24752856
[Au] Autor:Huberty M; Tielbörger K; Harvey JA; Müller C; Macel M
[Ad] Endereço:Plant Ecology, University of Tübingen, Auf der Morgenstelle 3, 72076, Tübingen, Germany.
[Ti] Título:Chemical defenses (glucosinolates) of native and invasive populations of the range expanding invasive plant Rorippa austriaca.
[So] Source:J Chem Ecol;40(4):363-70, 2014 Apr.
[Is] ISSN:1573-1561
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Due to global warming, species are expanding their range to higher latitudes. Some range expanding plants have become invasive in their new range. The Evolution of Increased Competitive Ability (EICA) hypothesis and the Shifting Defense Hypothesis (SDH) predict altered selection on plant defenses in the introduced range of invasive plants due to changes in herbivore pressures and communities. Here, we investigated chemical defenses (glucosinolates) of five native and seven invasive populations of the Eurasian invasive range expanding plant, Rorippa austriaca. Further, we studied feeding preferences of a generalist and a specialist herbivore among the populations. We detected eight glucosinolates in the leaves of R. austriaca. 8-Methylsulfinyloctyl glucosinolate was the most abundant glucosinolate in all plants. There were no overall differences between native and invasive plants in concentrations of glucosinolates. However, concentrations among populations within each range differed significantly. Feeding preference between the populations by a generalist herbivore was negatively correlated with glucosinolate concentrations. Feeding by a specialist did not differ between the populations and was not correlated with glucosinolates. Possibly, local differences in herbivore communities within each range may explain the differences in concentrations of glucosinolates among populations. Little support for the predictions of the EICA hypothesis or the SDH was found for the glucosinolate defenses of the studied native and invasive R. austriaca populations.
[Mh] Termos MeSH primário: Evolução Biológica
Borboletas/fisiologia
Cadeia Alimentar
Glucosinolatos/metabolismo
Mariposas/fisiologia
Rorippa/metabolismo
[Mh] Termos MeSH secundário: Animais
Borboletas/crescimento & desenvolvimento
Cromatografia Líquida de Alta Pressão
Europa (Continente)
Comportamento Alimentar
Espécies Introduzidas
Larva/fisiologia
Espectrometria de Massas
Mariposas/crescimento & desenvolvimento
Folhas de Planta/química
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucosinolates)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140423
[St] Status:MEDLINE
[do] DOI:10.1007/s10886-014-0425-1


  8 / 22 MEDLINE  
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[PMID]:24525961
[Au] Autor:Akman M; Bhikharie A; Mustroph A; Sasidharan R
[Ad] Endereço:Department of Plant Sciences; University of California; Davis, CA USA.
[Ti] Título:Extreme flooding tolerance in Rorippa.
[So] Source:Plant Signal Behav;9(2):e27847, 2014.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Low oxygen stress imposed by floods creates a strong selection force shaping plant ecosystems in flood-prone areas. Plants inhabiting these environments adopt various adaptations and survival strategies to cope with increasing water depths. Two Rorippa species, R. sylvestris and R. amphibia that grow in naturally flooded areas, have high submergence tolerance achieved by the so-called quiescence and escape strategies, respectively. In order to dissect the molecular mechanisms involved in these strategies, we investigated submergence-induced changes in gene expression in flooded roots of Rorippa species. There was a higher induction of glycolysis and fermentation genes and faster carbohydrate reduction in R. amphibia, indicating a higher demand for energy potentially leading to faster mortality by starvation. Moreover, R. sylvestris showed induction of genes improving submergence tolerance, potentially enhancing survival in prolonged floods. Additionally, we compared transcript profiles of these 2 tolerant species to relatively intolerant Arabidopsis and found that only Rorippa species induced various inorganic pyrophosphate dependent genes, alternatives to ATP demanding pathways, thereby conserving energy, and potentially explaining the difference in flooding survival between Rorippa and Arabidopsis.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Inundações
Rorippa/fisiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
Difosfatos/metabolismo
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Folhas de Planta/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Brotos de Planta/metabolismo
Rorippa/genética
Rorippa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diphosphates); 0 (Plant Proteins)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:140801
[Lr] Data última revisão:
140801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140215
[St] Status:MEDLINE


  9 / 22 MEDLINE  
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[PMID]:24077074
[Au] Autor:Sasidharan R; Mustroph A; Boonman A; Akman M; Ammerlaan AM; Breit T; Schranz ME; Voesenek LA; van Tienderen PH
[Ad] Endereço:Plant Ecophysiology, Institute for Environmental Biology, Utrecht University 3584CH, Utrecht, The Netherlands.
[Ti] Título:Root transcript profiling of two Rorippa species reveals gene clusters associated with extreme submergence tolerance.
[So] Source:Plant Physiol;163(3):1277-92, 2013 Nov.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Complete submergence represses photosynthesis and aerobic respiration, causing rapid mortality in most terrestrial plants. However, some plants have evolved traits allowing them to survive prolonged flooding, such as species of the genus Rorippa, close relatives of Arabidopsis (Arabidopsis thaliana). We studied plant survival, changes in carbohydrate and metabolite concentrations, and transcriptome responses to submergence of two species, Rorippa sylvestris and Rorippa amphibia. We exploited the close relationship between Rorippa species and the model species Arabidopsis by using Arabidopsis GeneChip microarrays for whole-genome transcript profiling of roots of young plants exposed to a 24-h submergence treatment or air. A probe mask was used based on hybridization of genomic DNA of both species to the arrays, so that weak probe signals due to Rorippa species/Arabidopsis mismatches were removed. Furthermore, we compared Rorippa species microarray results with those obtained for roots of submerged Arabidopsis plants. Both Rorippa species could tolerate deep submergence, with R. sylvestris surviving much longer than R. amphibia. Submergence resulted in the induction of genes involved in glycolysis and fermentation and the repression of many energy-consuming pathways, similar to the low-oxygen and submergence response of Arabidopsis and rice (Oryza sativa). The qualitative responses of both Rorippa species to submergence appeared roughly similar but differed quantitatively. Notably, glycolysis and fermentation genes and a gene encoding sucrose synthase were more strongly induced in the less tolerant R. amphibia than in R. sylvestris. A comparison with Arabidopsis microarray studies on submerged roots revealed some interesting differences and potential tolerance-related genes in Rorippa species.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Genes de Plantas/genética
Família Multigênica
Raízes de Plantas/genética
Rorippa/genética
Transcriptoma
[Mh] Termos MeSH secundário: Adaptação Fisiológica/fisiologia
Arabidopsis/genética
Arabidopsis/metabolismo
Inundações
Frutose/metabolismo
Regulação da Expressão Gênica de Plantas
Ontologia Genética
Glucose/metabolismo
Glicólise/genética
Dados de Sequência Molecular
Análise de Sequência com Séries de Oligonucleotídeos
Fotossíntese/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Raízes de Plantas/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Rorippa/classificação
Rorippa/metabolismo
Especificidade da Espécie
Amido/metabolismo
Sacarose/metabolismo
Água/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 059QF0KO0R (Water); 30237-26-4 (Fructose); 57-50-1 (Sucrose); 9005-25-8 (Starch); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131001
[St] Status:MEDLINE
[do] DOI:10.1104/pp.113.222588


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[PMID]:24040008
[Au] Autor:Bandopadhyay L; Basu D; Sikdar SR
[Ad] Endereço:Division of Plant Biology, Bose Institute, Centenary Campus, Kolkata, India.
[Ti] Título:Identification of genes involved in wild crucifer Rorippa indica resistance response on mustard aphid Lipaphis erysimi challenge.
[So] Source:PLoS One;8(9):e73632, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mustard aphid, Lipaphis erysimi (L.) Kaltenbach is a perpetual annual threat in the cultivation of rapeseed- mustard (Brassica spp.) crop in tropical and sub-tropical climate. Cultivated Brassica germplasm has failed so far to provide any source of resistance. Wild germplasm is a potential source of resistance against many threatening herbivores. On wild germplasm screening, we noted that the wild crucifer Rorippa indica (L.) Hiern confers resistance against L. erysimi. In the present study L. erysimi challenged transcriptome of R. indica was compared to un-infested R. indica sample to get a molecular insight about the aphid resistance mechanism and identify the candidate defense response genes. Cloning, sequencing and in silico sequence analysis of complimentary DNA amplified fragment length polymorphism identified 116 differentially expressed transcript derived fragments revealed thirty candidates which are from different functional categories including redox regulation, signalling, photosynthesis, structure, metabolism, defense response as well as a few of unknown function. Twenty four identifications were then studied by quantitative real time RT PCR analysis at 6, 12, 24 and 48 hour time point post infestation to understand the early-to-late defense response through their relative gene expression profiles. Seventeen fragments showed significant up or down regulation at p<0.05 level. The response was influenced by different phytohormonal signalling pathways simultaneously. The candidate defense response expressed sequence tags specifically for the resistance genes identified in this study have implication in building desired mustard aphid resistance in susceptible rapeseed-mustard plants in future. This is the first molecular report on crucifer defense response against mustard aphid L. erysimi.
[Mh] Termos MeSH primário: Afídeos/fisiologia
Resistência à Doença/genética
Genes de Plantas/genética
Rorippa/genética
Rorippa/parasitologia
[Mh] Termos MeSH secundário: Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Animais
DNA Complementar/química
DNA Complementar/genética
Etiquetas de Sequências Expressas
Regulação da Expressão Gênica de Plantas
Interações Hospedeiro-Parasita
Dados de Sequência Molecular
Mostardeira/parasitologia
Doenças das Plantas/genética
Doenças das Plantas/parasitologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de DNA
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130917
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0073632



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