Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.159.244 [Categoria DeCS]
Referências encontradas : 48 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 5 ir para página              

  1 / 48 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28199338
[Au] Autor:Brozio S; Manson C; Gourevitch E; Burns TJ; Greener MS; Downie JR; Hoskisson PA
[Ad] Endereço:Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.
[Ti] Título:Development and Application of an eDNA Method to Detect the Critically Endangered Trinidad Golden Tree Frog (Phytotriades auratus) in Bromeliad Phytotelmata.
[So] Source:PLoS One;12(2):e0170619, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The use of environmental DNA (eDNA) to monitor rare and elusive species has great potential for conservation biology. Traditional surveying methods can be time-consuming, labour-intensive, subject to error or can be invasive and potentially damaging to habitat. The Trinidad golden treefrog (Phytotriades auratus) is one such species that would benefit from such an approach. This species inhabits the giant bromeliad (Glomeropitcairnia erectiflora) on two peaks on the Caribbean island of Trinidad. Traditional survey methods for this species have required the destruction of the giant bromeliad, which is the only known habitat of this frog. Here we described the development of an eDNA PCR-based assay that uses water drawn from the water-filled phytotelmata of the giant bromeliad along with the use of a synthetic DNA positive control that can be easily amplified in the bacterium Escherichia coli. The assay can detect to a DNA concentration of 1.4ng. Sampling of 142 bromeliads using this method revealed 9% were positive for P. auratus DNA. These data suggest that eDNA methods also have great potential for revealing the presence of elusive species in arboreal habitats.
[Mh] Termos MeSH primário: Anuros/genética
Bromelia
DNA/genética
Espécies em Perigo de Extinção
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Animais
Trinidad e Tobago
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170619


  2 / 48 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo SciELO Saúde Pública
[PMID]:28125119
[Au] Autor:Oliveira VC; Almeida LC
[Ad] Endereço:Jardim Botânico Municipal de Bauru, Bauru, Brasil.
[Ti] Título:[Aedes aegypti and Aedes albopictus in bromeliads grown in the Bauru Municipal Botanical Gardens, São Paulo, Brazil].
[Ti] Título:Ocorrência de Aedes aegypti e Aedes albopictus em bromélias cultivadas no Jardim Botânico Municipal de Bauru, São Paulo, Brasil..
[So] Source:Cad Saude Publica;33(1):e00071016, 2017 Jan 23.
[Is] ISSN:1678-4464
[Cp] País de publicação:Brazil
[La] Idioma:por
[Ab] Resumo:The aim of this study was to observe the occurrence of mosquito larvae, especially Aedes aegypti and Aedes albopictus, in the tanks and axillae of bromeliads at the Bauru Municipal Botanical Gardens, São Paulo, Brazil, highlighting the epidemiological implications for the use of these plants. The majority of the larvae belonged to mosquitos from genus Culex, with only occasional findings of A. aegypti and A. albopictus. The use of screens for protection of the plants, exposure to sunlight, and larger amounts of water in the tanks may have influenced the occurrence and grouping of larvae.
[Mh] Termos MeSH primário: Aedes/classificação
Bromelia/parasitologia
Jardinagem
Insetos Vetores/classificação
[Mh] Termos MeSH secundário: Aedes/crescimento & desenvolvimento
Animais
Brasil
Dengue/transmissão
Insetos Vetores/crescimento & desenvolvimento
Larva
Densidade Demográfica
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE


  3 / 48 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27830465
[Au] Autor:Moreno-Hernández JM; Hernández-Mancillas XD; Navarrete ELC; Mazorra-Manzano MÁ; Osuna-Ruiz I; Rodríguez-Tirado VA; Salazar-Leyva JA
[Ad] Endereço:Programa de Investigación en Biotecnología. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Valle de Culiacán, Km. 17.5 Carretera Culiacán-Eldorado, 80000, Culiacán, SIN, Mexico.
[Ti] Título:Partial Characterization of the Proteolytic Properties of an Enzymatic Extract From "Aguama" Bromelia pinguin L. Fruit Grown in Mexico.
[So] Source:Appl Biochem Biotechnol;182(1):181-196, 2017 May.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant proteases are capable of performing several functions in biological systems, and their use is attractive for biotechnological process due to their interesting catalytic properties. Bromelia pinguin (aguama) is a wild abundant natural resource in several regions of Central America and the Caribbean Islands but is underutilized. Their fruits are rich in proteases with properties that are still unknown, but they represent an attractive source of enzymes for biotechnological applications. Thus, the proteolytic activity in enzymatic crude extracts (CEs) from wild B. pinguin fruits was partially characterized. Enzymes in CEs showed high proteolytic activity at acid (pH 2.0-4.0) and neutral alkaline (pH 7.0-9.0) conditions, indicating that different types of active proteases are present. Proteolytic activity inhibition by the use of specific protease inhibitors indicated that aspartic, cysteine, and serine proteases are the main types of proteases present in CEs. Activity at pH 3.0 was stable in a broad range of temperatures (25-50 °C) and retained its activity in the presence of surfactants (SDS, Tween-80), reducing agents (DTT, 2-mercapoethanol), and organic solvents (methanol, ethanol, acetone, 2-propanol), which suggests that B. pinguin proteases are potential candidates for their application in brewing, detergent, and pharmaceutical industries.
[Mh] Termos MeSH primário: Ácido Aspártico Proteases/química
Bromelia/enzimologia
Cisteína Proteases/química
Frutas/enzimologia
Proteínas de Plantas/química
Serina Proteases/química
[Mh] Termos MeSH secundário: Ácido Aspártico Proteases/antagonistas & inibidores
Ácido Aspártico Proteases/isolamento & purificação
Bromelia/química
Cisteína Proteases/isolamento & purificação
Ditiotreitol/química
Ensaios Enzimáticos
Frutas/química
Concentração de Íons de Hidrogênio
Cinética
Mercaptoetanol/química
Extratos Vegetais/química
Proteínas de Plantas/antagonistas & inibidores
Proteínas de Plantas/isolamento & purificação
Polissorbatos/química
Inibidores de Proteases/química
Proteólise
Serina Proteases/isolamento & purificação
Dodecilsulfato de Sódio/química
Solventes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Plant Proteins); 0 (Polysorbates); 0 (Protease Inhibitors); 0 (Solvents); 368GB5141J (Sodium Dodecyl Sulfate); 60-24-2 (Mercaptoethanol); EC 3.4.- (Aspartic Acid Proteases); EC 3.4.- (Cysteine Proteases); EC 3.4.- (Serine Proteases); T8ID5YZU6Y (Dithiothreitol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-016-2319-x


  4 / 48 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27400022
[Au] Autor:Egea-Serrano A; Solé M
[Ad] Endereço:Department of Biological Sciences, State University of Santa Cruz, Bahia, Brazil.
[Ti] Título:Effects of insecticides on a phytotelmata-breeding amphibian.
[So] Source:Environ Toxicol Chem;36(2):422-428, 2017 Feb.
[Is] ISSN:1552-8618
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Information on the impact of pesticides on amphibian species breeding in phytotelmata (water bodies within plants) is totally lacking. The aim of the present study was to assess the impact of the insecticide malathion on larvae of Phyllodytes luteolus. Individuals were exposed to ecologically relevant concentrations of malathion (commercial formulation) for 16 d under laboratory conditions. Malathion had a lethal effect that allowed the authors to hypothesize that phtytotelmata-breeding species are likely as vulnerable to pollution as pond breeders. Environ Toxicol Chem 2017;36:422-428. © 2016 SETAC.
[Mh] Termos MeSH primário: Anuros/crescimento & desenvolvimento
Monitoramento Ambiental/métodos
Inseticidas/toxicidade
Larva/efeitos dos fármacos
Malation/toxicidade
[Mh] Termos MeSH secundário: Animais
Brasil
Cruzamento
Bromelia/química
Ecologia
Inseticidas/análise
Larva/crescimento & desenvolvimento
Malation/análise
Praguicidas/farmacologia
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Pesticides); 059QF0KO0R (Water); U5N7SU872W (Malathion)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE
[do] DOI:10.1002/etc.3555


  5 / 48 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27686960
[Au] Autor:Chaves LSM; Rodrigues de Sá IL; Bergamaschi DP; Sallum MAM
[Ad] Endereço:Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, Av. Dr. Arnaldo, 715-Pacaembu, CEP - 01246-904 São Paulo, SP, Brazil. Electronic address: leonardosuveges@usp.br.
[Ti] Título:Kerteszia Theobald (Diptera: Culicidae) mosquitoes and bromeliads: A landscape ecology approach regarding two species in the Atlantic rainforest.
[So] Source:Acta Trop;164:303-313, 2016 Dec.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:On the ecological scale of an organism, a homogeneous geographical landscape can represent a mosaic of heterogeneous landscapes. The bionomy of Kerteszia mosquitoes can contribute to foundation landscape ecology by virtue of in the role of the configuration and composition of the habitat played in the distribution of mosquito species. Thus, this study aimed: to compare the abundance of Kerteszia in dense tropical rainforest, restinga and rural area, to assess the bioecological characteristics of the main bromeliads hosting Kerteszia, and to associate the bioecological arrangement of the bromeliads with Kerteszia distribution. Field collections were conducted in a monthly schedule from December of 2010 to November 2011. The vegetation of landscapes was characterized on the basis of a digital cartographic database, the manual of the Brazilian vegetation, environmental atlas information, satellite images and visits to the sites. Multivariate generalized linear models were employed using the R-project statistical program. The results were: Anopheles cruzii was the most frequent species in dense tropical rainforest (67.42%), with a positive association (deviance=25.8; P=0.002). Anopheles bellator was more abundant in the Restinga area (78.97%), with a positive association (deviance=10.4, P=0.018). There was a positive aggregation of Restinga with An. bellator (RR=2.42) but a lower level with An. cruzii (RR=0.31). Thus we can conclude that landscape characteristics influence the distribution of Kerteszia mosquitoes. An. bellator has a higher prevalence in Restinga areas, whereas An. cruzii was the most prevalent in the dense tropical rainforest.
[Mh] Termos MeSH primário: Anopheles/crescimento & desenvolvimento
Bromelia/crescimento & desenvolvimento
Ecossistema
Floresta Úmida
[Mh] Termos MeSH secundário: Animais
Biometria
Brasil
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE


  6 / 48 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27578765
[Au] Autor:Gonçalves AZ; Mercier H; Oliveira RS; Romero GQ
[Ad] Endereço:Pós-graduação em Ecologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, CEP 13083-970, Campinas, São Paulo, Brazil.
[Ti] Título:Trade-off between soluble protein production and nutritional storage in Bromeliaceae.
[So] Source:Ann Bot;118(6):1199-1208, 2016 Nov.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: Bromeliads are able to occupy some of the most nutrient-poor environments especially because they possess absorptive leaf trichomes, leaves organized in rosettes, distinct photosynthetic pathways [C , Crassulacean acid metabolism (CAM) or facultative C -CAM], and may present an epiphytic habit. The more derived features related to these traits are described for the Tillandsioideae subfamily. In this context, the aims of this study were to evaluate how terrestrial predators contribute to the nutrition and performance of bromeliad species, subfamilies and ecophysiological types, whether these species differ in their ecophysiological traits and whether the physiological outcomes are consistent among subfamilies and types (e.g. presence/absence of tank, soil/tank/atmosphere source of nutrients, trichomes/roots access to nutrients). METHODS: Isotopic ( N-enriched predator faeces) and physiological methods (analyses of plant protein, amino acids, growth, leaf mass per area and total N incorporated) in greenhouse experiments were used to investigate the ecophysiological contrasts between Tillandsioideae and Bromelioideae, and among ecophysiological types when a predatory anuran contributes to their nutrition. KEY RESULTS: It was observed that Bromelioideae had higher concentrations of soluble protein and only one species grew more (Ananas bracteatus), while Tillandsioideae showed higher concentrations of total amino acids, asparagine and did not grow. The ecophysiological types that showed similar protein contents also had similar growth. Additionally, an ordination analysis showed that the subfamilies and ecophysiological types were discrepant considering the results of the total nitrogen incorporated from predators, soluble protein and asparagine concentrations, relative growth rate and leaf mass per area. CONCLUSIONS: Bromeliad subfamilies showed a trade-off between two strategies: Tillandsioideae stored nitrogen into amino acids possibly for transamination reactions during nutritional stress and did not grow, whereas Bromelioideae used nitrogen for soluble protein production for immediate utilization, possibly for fast growth. These results highlight that Bromeliaceae evolution may be directly associated with the ability to stock nutrients.
[Mh] Termos MeSH primário: Bromeliaceae/metabolismo
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/metabolismo
Aminoácidos/fisiologia
Ananas/metabolismo
Ananas/fisiologia
Asparagina/metabolismo
Asparagina/fisiologia
Bromelia/metabolismo
Bromelia/fisiologia
Bromeliaceae/crescimento & desenvolvimento
Bromeliaceae/fisiologia
Isótopos de Nitrogênio/metabolismo
Folhas de Planta/metabolismo
Folhas de Planta/fisiologia
Fenômenos Fisiológicos Vegetais
Proteínas de Plantas/fisiologia
Tillandsia/metabolismo
Tillandsia/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Nitrogen Isotopes); 0 (Plant Proteins); 7006-34-0 (Asparagine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160901
[St] Status:MEDLINE


  7 / 48 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27272328
[Au] Autor:Ferreira RB; Beard KH; Crump ML
[Ad] Endereço:Department of Wildland Resources and the Ecology Center, Utah State University, Logan, Utah, United States of America.
[Ti] Título:Breeding Guild Determines Frog Distributions in Response to Edge Effects and Habitat Conversion in the Brazil's Atlantic Forest.
[So] Source:PLoS One;11(6):e0156781, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding the response of species with differing life-history traits to habitat edges and habitat conversion helps predict their likelihood of persistence across changing landscape. In Brazil's Atlantic Forest, we evaluated frog richness and abundance by breeding guild at four distances from the edge of a reserve: i) 200 m inside the forest, ii) 50 m inside the forest, iii) at the forest edge, and iv) 50 m inside three different converted habitats (coffee plantation, non-native Eucalyptus plantation, and abandoned pastures, hereafter matrix types). By sampling a dry and a wet season, we recorded 622 individual frogs representing 29 species, of which three were undescribed. Breeding guild (i.e. bromeliad, leaf-litter, and water-body breeders) was the most important variable explaining frog distributions in relation to edge effects and matrix types. Leaf-litter and bromeliad breeders decreased in richness and abundance from the forest interior toward the matrix habitats. Water-body breeders increased in richness toward the matrix and remained relatively stable in abundance across distances. Number of large trees (i.e. DBH > 15 cm) and bromeliads best explained frog richness and abundance across distances. Twenty species found in the interior of the forest were not found in any matrix habitat. Richness and abundance across breeding guilds were higher in the rainy season but frog distributions were similar across the four distances in the two seasons. Across matrix types, leaf-litter species primarily used Eucalyptus plantations, whereas water-body species primarily used coffee plantations. Bromeliad breeders were not found inside any matrix habitat. Our study highlights the importance of primary forest for bromeliad and leaf-litter breeders. We propose that water-body breeders use edge and matrix habitats to reach breeding habitats along the valleys. Including life-history characteristics, such as breeding guild, can improve predictions of frog distributions in response to edge effect and matrix types, and can guide more effective management and conservation actions.
[Mh] Termos MeSH primário: Anuros/fisiologia
Cruzamento/métodos
Bromelia/fisiologia
Café/fisiologia
Eucalyptus/fisiologia
[Mh] Termos MeSH secundário: Animais
Anuros/classificação
Brasil
Conservação dos Recursos Naturais
Ecossistema
Florestas
Folhas de Planta/fisiologia
Densidade Demográfica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coffee)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0156781


  8 / 48 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27002980
[Au] Autor:Gonçalves AZ; Oliveira RS; Oliveira PS; Romero GQ
[Ad] Endereço:Pós-graduação em Ecologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, CEP 13083-970, Campinas, São Paulo, Brazil.
[Ti] Título:Species-Specific Effects of Ant Inhabitants on Bromeliad Nutrition.
[So] Source:PLoS One;11(3):e0152113, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Predator activities may lead to the accumulation of nutrients in specific areas of terrestrial habitats where they dispose of prey carcasses. In their feeding sites, predators may increase nutrient availability in the soil and favor plant nutrition and growth. However, the translocation of nutrients from one habitat to another may depend on predator identity and diet, as well as on the amount of prey intake. Here we used isotopic (15N) and physiological methods in greenhouse experiments to evaluate the effects of the identity of predatory ants (i.e., the consumption of prey and nest sites) on the nutrition and growth of the bromeliad Quesnelia arvensis. We showed that predatory ants with protein-based nutrition (i.e., Odontomachus hastatus, Gnamptogenys moelleri) improved the performance of their host bromeliads (i.e., increased foliar N, production of soluble proteins and growth). On the other hand, the contribution of Camponotus crassus for the nutritional status of bromeliads did not differ from bromeliads without ants, possibly because this ant does not have arthropod prey as a preferred food source. Our results show, for the first time, that predatory ants can translocate nutrients from one habitat to another within forests, accumulating nutrients in their feeding sites that become available to bromeliads. Additionally, we highlight that ant contribution to plant nutrition may depend on predator identity and its dietary requirements. Nest debris may be especially important for epiphytic and terrestrial bromeliads in nutrient-poor environments.
[Mh] Termos MeSH primário: Formigas/fisiologia
Bromelia/fisiologia
Comportamento Alimentar/fisiologia
Comportamento Predatório/fisiologia
[Mh] Termos MeSH secundário: Animais
Formigas/metabolismo
Bromelia/metabolismo
Ecossistema
Alimentos
Nitrogênio/metabolismo
Estado Nutricional/fisiologia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
N762921K75 (Nitrogen)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160323
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0152113


  9 / 48 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26886361
[Au] Autor:Errasti ME; Prospitti A; Viana CA; Gonzalez MM; Ramos MV; Rotelli AE; Caffini NO
[Ad] Endereço:aCenter for Plant Protein Research, Faculty of Exact Sciences, National University of La Plata, La Plata, ArgentinabDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, BrazilcLaboratory of Hematology, Faculty of Exact Sciences, National University of La Plata, La PlatadLaboratory of Pharmacology, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, San Luis, Argentina.
[Ti] Título:Effects on fibrinogen, fibrin, and blood coagulation of proteolytic extracts from fruits of Pseudananas macrodontes, Bromelia balansae, and B. hieronymi (Bromeliaceae) in comparison with bromelain.
[So] Source:Blood Coagul Fibrinolysis;27(4):441-9, 2016 Jun.
[Is] ISSN:1473-5733
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Extracts rich in cysteine proteases obtained from fruits of Pseudananas macrodontes (Pm), Bromelia balansae (Bb), and B. hieronymi (Bh) have previously shown an anti-inflammatory effect on animal models. Given the close relationship between hemostasis and inflammation, it is attractive to investigate therapeutic agents capable of modulating both systems. The aim of this work was to study the effect of Pm, Bb, and Bh on fibrin(ogen) and blood coagulation compared with stem bromelain (Bro). Action on fibrinogen was electrophoretically and spectrophotometrically evaluated, fibrinolytic activity was measured both electrophoretically and by the fibrin plate assay, and the effect on blood coagulation was studied by conventional coagulation tests (PT and APPT). All extracts showed the same proteolytic preference for fibrinogen subunits, that is Aα > Bß, whereas γ was partially hydrolyzed by 100-fold concentration increase. Unlike Bro, cysteine proteases of Pm, Bb, and Bh increased absorbance at 540 nm of fibrinogen solution, suggesting thrombin-like activity, which was time-dependent and reached maximum values at lower concentration. All extracts showed the same proteolytic preference for fibrin subunits; however Pm, Bb, and Bh showed lower fibrinolytic activity than Bro at the assayed concentrations. Although Bb acted only as anticoagulant, Pm, Bh, and unexpectedly Bro showed dual action on blood coagulation: at low concentration showed procoagulant effect and at high concentration anticoagulant effect. Results reveal new plant species as potential sources of pharmacological agents for the treatment of a wide range of hemostatic disorders as well as to wound healing.
[Mh] Termos MeSH primário: Coagulação Sanguínea/efeitos dos fármacos
Bromelia/química
Bromeliaceae/química
Fibrina/química
Fibrinogênio/química
Fibrinolíticos/farmacologia
Frutas/química
[Mh] Termos MeSH secundário: Células Sanguíneas/efeitos dos fármacos
Testes de Coagulação Sanguínea
Bromelaínas/química
Eletroforese em Gel de Poliacrilamida
Produtos de Degradação da Fibrina e do Fibrinogênio/química
Fibrinolíticos/química
Fibrinolíticos/isolamento & purificação
Hormese
Seres Humanos
Extratos Vegetais/química
Cultura Primária de Células
Proteólise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fibrin Fibrinogen Degradation Products); 0 (Fibrinolytic Agents); 0 (Plant Extracts); 9001-00-7 (Bromelains); 9001-31-4 (Fibrin); 9001-32-5 (Fibrinogen)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160218
[St] Status:MEDLINE
[do] DOI:10.1097/MBC.0000000000000531


  10 / 48 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:26871497
[Au] Autor:Motta-Tavares T; Maia-Carneiro T; Dantas LF; Sluys MV; Hatano FH; Vrcibradic D; Rocha CF
[Ad] Endereço:Departamento de Ecologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
[Ti] Título:Ecology of the bromeligenous frog Phyllodytes luteolus (Anura, Hylidae) from three restinga remnants across Brazil's coast.
[So] Source:An Acad Bras Cienc;88(1):93-104, 2016 Mar.
[Is] ISSN:1678-2690
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:In this study, we analyzed diet, sexual dimorphism and bromeliad use in three populations of the hylid frog Phyllodytes luteolus from restinga habitats along the Brazilian coast. We found 13 arthropods categories in 161 stomachs. Ants and termites were the dominant prey items. The similar trophic niche across populations suggests this species has a conservative diet. We found sexual dimorphism regarding body size and jaw width. We recordedP. luteolus in five bromeliad species, but predominantly inAechmeablanchetiana (35.6% of individuals recorded). We recorded solitary individuals in 44% of occupied bromeliads, and never found two males sharing the same bromeliad. The data is suggestive that populations ofP. luteolus has a conservative diet independent of area, with ants and termites the being most relevant prey items. The sexual dimorphism in jaw and the solitary males may suggest that this species have territorial behavior.
[Mh] Termos MeSH primário: Anuros/fisiologia
Comportamento Animal/fisiologia
Comportamento Alimentar/fisiologia
Caracteres Sexuais
[Mh] Termos MeSH secundário: Animais
Anuros/classificação
Brasil
Bromelia
Feminino
Conteúdo Gastrointestinal
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE



página 1 de 5 ir para página              
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde