Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.165.750 [Categoria DeCS]
Referências encontradas : 12 [refinar]
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  1 / 12 MEDLINE  
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[PMID]:25414071
[Au] Autor:Zhao H; Wang XY; Li MK; Hou Z; Zhou Y; Chen Z; Meng JR; Luo XX; Tang HF; Xue XY
[Ad] Endereço:Student Company in the College of Pharmacy, The Fourth Military Medical University, No. 169, Changle West Road, Xi'an, 710032, China.
[Ti] Título:A novel pregnane-type alkaloid from Pachysandra terminalis inhibits methicillin-resistant Staphylococcus aureus in vitro and in vivo.
[So] Source:Phytother Res;29(3):373-80, 2015 Mar.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new kind of pregnane-type alkaloid, 20α-dimethylamino-3ß-senecioylamino-16ß-hydroxy-pregn-5-ene (K-6), was isolated from Pachysandra terminalis Sieb. et Zucc., and its antibacterial activity against MRSA and MRSE was evaluated. We found that K-6 showed antibacterial effects against MRSA and MRSE with minimum inhibitory concentration values (25 mg/L), but did not induce antibiotic resistance in bacteria easily. The administration of K-6 dose-dependently improved the animal survival rate of mice infected with MRSA, with survival rates of 36.34% and 66.67% in the low-dose and high-dose groups, respectively. The protective effects were associated with the reduction of the bacterial titers in the blood and with the morphological amelioration of infected tissues. Scanning and transmission electron microscopy analyses indicated that the cytoplasm shrink of bacterial cells led to noticeable gaps between the cell membrane and cell cytoplasm, and the severely damaged cell membrane resulted in leakage of intracellular content, which ultimately caused the lethal effect of K-6 on bacteria. These findings demonstrated that K-6 is a potential agent against MRSA and MRSE.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Antibacterianos/farmacologia
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Pachysandra/química
Pregnanos/farmacologia
[Mh] Termos MeSH secundário: Animais
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (20alpha-dimethylamino-3beta-senecioylamino-16beta-hydroxy-pregn-5-ene); 0 (Alkaloids); 0 (Anti-Bacterial Agents); 0 (Pregnanes)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150310
[Lr] Data última revisão:
150310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141122
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5261


  2 / 12 MEDLINE  
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[PMID]:25178723
[Au] Autor:Verspreet J; Holmgaard Hansen A; Dornez E; Delcour JA; Harrison SJ; Courtin CM
[Ad] Endereço:Laboratory of Food Chemistry and Biochemistry, Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, 3001, Leuven, Belgium.
[Ti] Título:Liquid chromatography/mass spectrometry analysis of branched fructans produced in vitro with 13C-labeled substrates.
[So] Source:Rapid Commun Mass Spectrom;28(20):2191-200, 2014 Oct 30.
[Is] ISSN:1097-0231
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Fructans are carbohydrates predominantly based on fructose which are generally considered to be soluble dietary fibers with health-promoting properties. It is known that the nutritional properties of fructans are affected by their structure. This study focused on structural determination of branched fructans, as the most important dietary fructans are branched graminan-type fructans. METHODS: Branched fructans were synthesized enzymatically by incubation of a heterologously expressed sucrose:fructan 6-fructosyltransferase (6-SFT) from Pachysandra terminalis with native or (13)C-labeled substrates. Liquid chromatography/mass spectrometry (LC/MS) was used for the structural identification of branched fructans. The MS(2) fragmentation of these compounds is described for the first time. Analytes were charged by electrospray ionization in negative mode and a quadrupole mass analyzer was used for MS(2) analysis. RESULTS: The MS(2) fragmentation patterns of branched and linear fructans were shown to differ and distinctive ion formation allowed differentiation between all branched fructan isomers formed. P. terminalis 6-SFT preferred extending the existing fructan branch rather than creating a new branch. CONCLUSIONS: The MS(2) fragmentation patterns described in the current paper now allow rapid screening of large sample sets for the presence of branched, graminan-type fructans. Furthermore, the data enables the characterization of fructan-metabolizing enzymes by identification of the fructan structures produced by in vitro reactions as described here for P. terminalis 6-SFT.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Frutanos/análise
Frutanos/química
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Configuração de Carboidratos
Isótopos de Carbono/análise
Isótopos de Carbono/química
Isótopos de Carbono/metabolismo
Frutanos/metabolismo
Hexosiltransferases/metabolismo
Modelos Moleculares
Pachysandra/metabolismo
Proteínas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (Fructans); 0 (Plant Proteins); EC 2.4.1.- (Hexosyltransferases)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:140902
[Lr] Data última revisão:
140902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140903
[St] Status:MEDLINE
[do] DOI:10.1002/rcm.7013


  3 / 12 MEDLINE  
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[PMID]:24648170
[Au] Autor:Wang HL; Qin N; Liu J; Jin MN; Zhang X; Jin MH; Kong D; Jiang SD; Duan HQ
[Ad] Endereço:School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China.
[Ti] Título:Synthesis and antimetastatic effects of E-salignone amide derivatives.
[So] Source:Drug Dev Res;75(2):76-87, 2014 Mar.
[Is] ISSN:1098-2299
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The preparation of novel E-salignone derivatives and their biological evaluation as potential antimetastatic agents is described. The E-salignone amide derivatives were prepared from epiandrosterone and androsterone, and characterized by analytical (1) H NMR, (13) C NMR, and mass spectrometry. The derivatives were evaluated for antimetastatic activity in MDA-MB-231 cells by using a transwell assay. Comparing with the positive control, LY294002, compounds 19b, 19d, and 19e exhibited significant inhibitory effects on the EGF-induced invasion of MB-MDA-231 cells. Moreover, compound 19b also had antimigration effects in wound-healing assay. Compound 19b may represent a novel antimetastatic agent for treating breast cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/síntese química
Antineoplásicos Fitogênicos/farmacologia
Neoplasias da Mama/prevenção & controle
Pregnenos/síntese química
Pregnenos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Neoplasias da Mama/patologia
Movimento Celular/efeitos dos fármacos
Cromonas/farmacologia
Feminino
Seres Humanos
Estrutura Molecular
Morfolinas/farmacologia
Invasividade Neoplásica
Metástase Neoplásica
Pachysandra/química
Pregnenos/química
Pregnenos/isolamento & purificação
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Chromones); 0 (Morpholines); 0 (Pregnenes); 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140321
[St] Status:MEDLINE
[do] DOI:10.1002/ddr.21157


  4 / 12 MEDLINE  
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[PMID]:24625225
[Au] Autor:Zhao C; Gan CC; Jin MN; Tang SA; Qin N; Duan HQ
[Ad] Endereço:a Research Center of Basic Medical Sciences, Tianjin Medical University , Tianjin 300070 , China.
[Ti] Título:Antitumor metastasis pregnane alkaloids from Pachysandra terminalis.
[So] Source:J Asian Nat Prod Res;16(5):440-6, 2014.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three new pregnane alkaloids, named terminamines H-J (1-3), together with two known alkaloids (4 and 5), were isolated from the ethanol extract of Pachysandra terminalis. The structures of isolated compounds were elucidated by spectroscopic methods, including (1)H and (13)C NMR, 2D NMR, and HR-ESI-MS. Compounds 1, 4, and 5 revealed significant anti-metastasis activities. In addition, compound 1 inhibited the expression of p-PKCζ in MDA-MB-231 cells, and compound 4 inhibited the expressions of p-PKCζ in MDA-MB-231 and A549 cells.
[Mh] Termos MeSH primário: Alcaloides/isolamento & purificação
Alcaloides/farmacologia
Pachysandra/química
Pregnanos/isolamento & purificação
[Mh] Termos MeSH secundário: Alcaloides/química
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Pregnanos/química
Pregnanos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Antineoplastic Agents, Phytogenic); 0 (Pregnanes); 0 (terminamine H); 0 (terminamine I); 0 (terminamine J)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:140514
[Lr] Data última revisão:
140514
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140315
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2014.893511


  5 / 12 MEDLINE  
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[PMID]:23668007
[Au] Autor:Xiao HJ; Li CY; Tang SA; Qin N; Duan HQ
[Ad] Endereço:School of Pharmacy, Research Center of Basic Medical Sciences, Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics, Tianjin Medical University, Tianjin 300070, China. hedy_xiao@163.com
[Ti] Título:[Chemical constituents from Pachysandra terminalis].
[So] Source:Zhongguo Zhong Yao Za Zhi;38(3):350-3, 2013 Feb.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To study chemical constituents from Pachysandra terminalis. By repeated column chromatography, including silica gel, Toyopearl HW-40, and preparative HPLC, four new (14) and one known (5) compounds were isolated and purified. On the basis of spectral data analysis, the structure of isolated compounds were elucidated as follow: 2-methyl-3-methylenepentane-1, 2, 5-triol (1), 4-methyl-3-methylenepentane-1, 2, 5-triol (2), 4-methyl-3-methylenepentane-1, 2, 5-triol-5-O-beta-D-glucopyranoside (3), 4-methyl-3-methylenep- entane-1, 2, 5-triol-1-O-beta-D-glucopyranoside (4), (7S, 8R, 8' R)-(+)-lariciresinol-9-O-beta-D-glucopyrano-side (5). Compound 5 was isolated from this genus for the first time.
[Mh] Termos MeSH primário: Glucosídeos/química
Pachysandra/química
Extratos Vegetais/química
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Cromatografia em Gel
Cromatografia Líquida de Alta Pressão
Glucosídeos/isolamento & purificação
Estrutura Molecular
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucosides); 0 (Plant Extracts); 0 (lariciresinol-9-O-beta-D-glucopyranoside)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:130514
[Lr] Data última revisão:
130514
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130515
[St] Status:MEDLINE


  6 / 12 MEDLINE  
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[PMID]:22804108
[Au] Autor:Zhai HY; Zhao C; Zhang N; Jin MN; Tang SA; Qin N; Kong DX; Duan HQ
[Ad] Endereço:Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University , Tianjin 300070, People's Republic of China.
[Ti] Título:Alkaloids from Pachysandra terminalis inhibit breast cancer invasion and have potential for development as antimetastasis therapeutic agents.
[So] Source:J Nat Prod;75(7):1305-11, 2012 Jul 27.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of the present study was to identify potentially useful natural compounds for the development of novel therapeutic agents to inhibit metastasis. A phytochemical investigation of Pachysandra terminalis resulted in the isolation of seven new pregnane alkaloids, terminamines A-G (1-7), and seven known alkaloids (8-14). The structures of 1-7 were elucidated by 1D- and 2D-NMR spectroscopic and mass spectrometric methods. Compounds 1-5 and 8-14 inhibited the migration of MB-MDA-231 breast cancer cells induced by the chemokine epithelial growth factor. In addition, compound 1 inhibited phosphorylation of integrin ß(1), which plays an important role in MB-MDA-231 cell adhesion and metastasis.
[Mh] Termos MeSH primário: Alcaloides/isolamento & purificação
Alcaloides/farmacologia
Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Medicamentos de Ervas Chinesas/isolamento & purificação
Medicamentos de Ervas Chinesas/farmacologia
Pachysandra/química
Pregnanos/isolamento & purificação
Pregnanos/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/química
Antineoplásicos/química
Neoplasias da Mama/tratamento farmacológico
Medicamentos de Ervas Chinesas/química
Feminino
Seres Humanos
Estrutura Molecular
Metástase Neoplásica/tratamento farmacológico
Ressonância Magnética Nuclear Biomolecular
Pregnanos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Antineoplastic Agents); 0 (Drugs, Chinese Herbal); 0 (Pregnanes)
[Em] Mês de entrada:1211
[Cu] Atualização por classe:120727
[Lr] Data última revisão:
120727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120719
[St] Status:MEDLINE
[do] DOI:10.1021/np300207c


  7 / 12 MEDLINE  
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[PMID]:22098191
[Au] Autor:Lammens W; Le Roy K; Yuan S; Vergauwen R; Rabijns A; Van Laere A; Strelkov SV; Van den Ende W
[Ad] Endereço:Biology Department, Laboratory for Molecular Plant Physiology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31, Box 2434, B-3001 Heverlee, Belgium.
[Ti] Título:Crystal structure of 6-SST/6-SFT from Pachysandra terminalis, a plant fructan biosynthesizing enzyme in complex with its acceptor substrate 6-kestose.
[So] Source:Plant J;70(2):205-19, 2012 Apr.
[Is] ISSN:1365-313X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fructans play important roles as reserve carbohydrates and stress protectants in plants, and additionally serve as prebiotics with emerging antioxidant properties. Various fructan types are synthesized by an array of plant fructosyltransferases belonging to family 32 of the glycoside hydrolases (GH32), clustering together with GH68 in Clan-J. Here, the 3D structure of a plant fructosyltransferase from a native source, the Pachysandra terminalis 6-SST/6-SFT (Pt6-SST/6-SFT), is reported. In addition to its 1-SST (1-kestose-forming) and hydrolytic side activities, the enzyme uses sucrose to create graminan- and levan-type fructans, which are probably associated with cold tolerance in this species. Furthermore, a Pt6-SST/6-SFT complex with 6-kestose was generated, representing a genuine acceptor binding modus at the +1, +2 and +3 subsites in the active site. The enzyme shows a unique configuration in the vicinity of its active site, including a unique D/Q couple located at the +1 subsite that plays a dual role in donor and acceptor substrate binding. Furthermore, it shows a unique orientation of some hydrophobic residues, probably contributing to its specific functionality. A model is presented showing formation of a ß(2-6) fructosyl linkage on 6-kestose to create 6,6-nystose, a mechanism that differs from the creation of a ß(2-1) fructosyl linkage on sucrose to produce 1-kestose. The structures shed light on the evolution of plant fructosyltransferases from their vacuolar invertase ancestors, and contribute to further understanding of the complex structure-function relationships within plant GH32 members.
[Mh] Termos MeSH primário: Frutanos/biossíntese
Hexosiltransferases/metabolismo
Pachysandra/enzimologia
Proteínas de Plantas/metabolismo
Trissacarídeos/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sítios de Ligação/genética
Domínio Catalítico
Cristalografia por Raios X
Hexosiltransferases/química
Hexosiltransferases/genética
Cinética
Modelos Moleculares
Dados de Sequência Molecular
Pachysandra/genética
Pachysandra/metabolismo
Proteínas de Plantas/química
Proteínas de Plantas/genética
Ligação Proteica
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
Trissacarídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fructans); 0 (Plant Proteins); 0 (Trisaccharides); 02LN7O412C (1-kestose); 562-68-5 (6-kestose); EC 2.4.1.- (Hexosyltransferases); EC 2.4.1.9 (inulosucrase)
[Em] Mês de entrada:1208
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111122
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-313X.2011.04858.x


  8 / 12 MEDLINE  
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[PMID]:21037113
[Au] Autor:Van den Ende W; Coopman M; Clerens S; Vergauwen R; Le Roy K; Lammens W; Van Laere A
[Ad] Endereço:Laboratory of Molecular Plant Physiology, Institute of Botany and Microbiology, KU Leuven, B-3001 Leuven, Belgium. wim.vandenende@bio.kuleuven
[Ti] Título:Unexpected presence of graminan- and levan-type fructans in the evergreen frost-hardy eudicot Pachysandra terminalis (Buxaceae): purification, cloning, and functional analysis of a 6-SST/6-SFT enzyme.
[So] Source:Plant Physiol;155(1):603-14, 2011 Jan.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:About 15% of flowering plants accumulate fructans. Inulin-type fructans with ß(2,1) fructosyl linkages typically accumulate in the core eudicot families (e.g. Asteraceae), while levan-type fructans with ß(2,6) linkages and branched, graminan-type fructans with mixed linkages predominate in monocot families. Here, we describe the unexpected finding that graminan- and levan-type fructans, as typically occurring in wheat (Triticum aestivum) and barley (Hordeum vulgare), also accumulate in Pachysandra terminalis, an evergreen, frost-hardy basal eudicot species. Part of the complex graminan- and levan-type fructans as accumulating in vivo can be produced in vitro by a sucrose:fructan 6-fructosyltransferase (6-SFT) enzyme with inherent sucrose:sucrose 1-fructosyltransferase (1-SST) and fructan 6-exohydrolase side activities. This enzyme produces a series of cereal-like graminan- and levan-type fructans from sucrose as a single substrate. The 6-SST/6-SFT enzyme was fully purified by classic column chromatography. In-gel trypsin digestion led to reverse transcription-polymerase chain reaction-based cDNA cloning. The functionality of the 6-SST/6-SFT cDNA was demonstrated after heterologous expression in Pichia pastoris. Both the recombinant and native enzymes showed rather similar substrate specificity characteristics, including peculiar temperature-dependent inherent 1-SST and fructan 6-exohydrolase side activities. The finding that cereal-type fructans accumulate in a basal eudicot species further confirms the polyphyletic origin of fructan biosynthesis in nature. Our data suggest that the fructan syndrome in P. terminalis can be considered as a recent evolutionary event. Putative connections between abiotic stress and fructans are discussed.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Congelamento
Frutanos/metabolismo
Hexosiltransferases/genética
Hexosiltransferases/isolamento & purificação
Pachysandra/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Cromatografia por Troca Iônica
Clonagem Molecular
DNA Complementar/genética
Eletroforese em Gel de Poliacrilamida
Evolução Molecular
Hexosiltransferases/química
Hexosiltransferases/metabolismo
Concentração de Íons de Hidrogênio
Hidrólise
Dados de Sequência Molecular
Peso Molecular
Pachysandra/genética
Mapeamento de Peptídeos
Filogenia
Pichia/metabolismo
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Fructans); 9013-95-0 (levan); EC 2.4.1.- (Hexosyltransferases); EC 2.4.1.9 (inulosucrase)
[Em] Mês de entrada:1104
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101102
[St] Status:MEDLINE
[do] DOI:10.1104/pp.110.162222


  9 / 12 MEDLINE  
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[PMID]:20939276
[Au] Autor:Zhai H; Li C; Tang S; Duan H
[Ad] Endereço:School of Parmaceutical Sciences, Basic Medical Research Center, Tianjin Medical University, Tianjin 300070, China. zhyyihun@163.com
[Ti] Título:[Phenol constituents of Pachysandra terminalis and their antioxidant activity].
[So] Source:Zhongguo Zhong Yao Za Zhi;35(14):1820-3, 2010 Jul.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To study the phenol constituents from Pachysandra terminalis and their antioxidant activities. METHOD: Constituent isolation and purification was carried by repeated column chromatography (silica gel, Toyopearl HW-40 and preparative HPLC), and their structures were elucidated on the basis of spectral data analysis. DPPH method was used to evaluate the free radical scavenging activity of the isolated compounds. RESULT: Nine phenol compounds (1-9) were isolated and their structures were identified as follow: p-hydroxybenzaldehyde (1), vanillin (2), 1-(4-hydroxy-3-methoxyphenyl) -ethanone (3), syringaldehyde (4), salicylic acid (5), p-hydroxybenzoic acid (6), ferulic acid (7), 2,3,4-trihydroxybenzoic acid (8), 3,4-dihydroxybenzoic acid (9). The isolated compounds showed obviously antioxidant activity. At the concentration of 50 micromol x L(-1), compounds 7-9 revealed DPPH free radical scavenging rates were 87.8%, 97.8% and 92%, respectively. CONCLUSION: Compounds 1-9 were isolated from this genus for the first time. They showed the significant antioxidant activity.
[Mh] Termos MeSH primário: Antioxidantes/análise
Pachysandra/química
Fenóis/análise
Extratos Vegetais/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Phenols); 0 (Plant Extracts)
[Em] Mês de entrada:1011
[Cu] Atualização por classe:101013
[Lr] Data última revisão:
101013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101014
[St] Status:MEDLINE


  10 / 12 MEDLINE  
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[PMID]:20873557
[Au] Autor:Li CY; Zhai HY; Tang SA; Duan HQ
[Ad] Endereço:School of Pharmaceutical Sciences, Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China. licy0609@126.com
[Ti] Título:[Studies on the chemical constituents of Pachysandra terminalis and their antioxidant activity].
[So] Source:Zhong Yao Cai;33(5):729-32, 2010 May.
[Is] ISSN:1001-4454
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To study the chemical constituents from Pachysandra terminalis and their antioxidant activity. METHODS: Chemical constituents were isolated by repeated column chromatography (silica gel, Toyopearl HW-40C and preparative HPLC). The structures of isolated compounds were elucidated on the basis of spectral data analysis. DPPH method was used to evaluate the free radical scavenging activity of the isolated compounds. RESULTS: Six compounds were isolated and their structures were identified as follow: 2-Phenylethyl-beta-D-glucopyranoside (1), (+)Pinoresinol-4'-O-beta-D-glucopyranoside (2), Pinoresinol (3), cis-Syringin (4), 4-hydroxy-4-(3-oxo-l-butenyl)-3,5,5-trimethylcyclohex-2-en-l-one (5), 3alpha-hydroxy-5,6-epoxy-7-megastigmen-9-one (6). Compounds 2, 4, 5 showed obviously antioxidant activity, their DPPH free radical scavenging rates were 82.50%, 87.36%, and 84.56% on the concentration of 50 micromol/L, respectively. CONCLUSION: Compounds 1-6 are isolated from this genus for the first time. Compounds 2, 4, and 5 showed significant antioxidant activities.
[Mh] Termos MeSH primário: Antioxidantes/análise
Furanos/análise
Glicosídeos/análise
Lignanas/análise
Pachysandra/química
Álcool Feniletílico/análogos & derivados
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Antioxidantes/isolamento & purificação
Compostos de Bifenilo/metabolismo
Cromatografia Líquida de Alta Pressão
Depuradores de Radicais Livres
Furanos/isolamento & purificação
Glucosídeos/análise
Glucosídeos/isolamento & purificação
Glicosídeos/isolamento & purificação
Lignanas/isolamento & purificação
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Álcool Feniletílico/análise
Álcool Feniletílico/isolamento & purificação
Fenilpropionatos/análise
Fenilpropionatos/isolamento & purificação
Picratos/metabolismo
Extratos Vegetais/análise
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Free Radical Scavengers); 0 (Furans); 0 (Glucosides); 0 (Glycosides); 0 (Lignans); 0 (Phenylpropionates); 0 (Picrates); 0 (Plant Extracts); 0 (pinoresinol 4-O-glucopyranoside); 129932-48-5 (phenethyl alcohol xylopyranosyl-(1-6)-glucopyranoside); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); I6F5B11C96 (syringin); ML9LGA7468 (Phenylethyl Alcohol); V4N1UDY811 (pinoresinol)
[Em] Mês de entrada:1103
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100930
[St] Status:MEDLINE



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