Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.171 [Categoria DeCS]
Referências encontradas : 176 [refinar]
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  1 / 176 MEDLINE  
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[PMID]:28829825
[Au] Autor:Cheon KS; Kim KA; Yoo KO
[Ad] Endereço:Department of Biological Sciences, Kangwon National University, Chuncheon, Gangwon, South Korea.
[Ti] Título:The complete chloroplast genome sequences of three Adenophora species and comparative analysis with Campanuloid species (Campanulaceae).
[So] Source:PLoS One;12(8):e0183652, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We report the complete chloroplast genomes of three Adenophora species, and analyzed these compared them to five published Campanuloid plastomes. The total genome length of Adenophora divaricata, Adenophora erecta, and Adenophora stricta ranged from 159,759 to 176,331 bp. Among the eight Campanuloid species, many inversions were found to be only in the LSC region. IR contraction was also identified in the plastid genome of Adenophora stricta. Phylogenetic analyses based on 76 protein coding genes showed that Campanuloids are monophyletic, and are composed of two major groups: Campanula s. str. and Rapunculus. When we compared each homologous locus among the four Adenophora species, ten regions showed high nucleotide divergence value (>0.03). Among these, nine loci, excepting ycf3-rpoB, are considered to be useful molecular markers for phylogenetic studies and will be helpful to resolve phylogenetic relationships of Adenophora.
[Mh] Termos MeSH primário: Campanulaceae/genética
Cloroplastos/genética
Genoma de Planta
[Mh] Termos MeSH secundário: Campanulaceae/classificação
Marcadores Genéticos
Filogenia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183652


  2 / 176 MEDLINE  
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[PMID]:28597166
[Au] Autor:Chen J; Niu Y; Li Z; Yang Y; Sun H
[Ad] Endereço:Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China.
[Ti] Título:Sex allocation in gynodioecious Cyananthus delavayi differs between gender morphs and soil quality.
[So] Source:Plant Reprod;30(2):107-117, 2017 Jun.
[Is] ISSN:2194-7961
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Sex allocation in Cyananthus delavayi. Gynodioecy, where females and hermaphrodites coexist in the same natural population, is particularly suitable for predicting the ecological pressures that drive the stability of gender polymorphism. Since females have a disadvantage in that they only contribute to the next generation via ovules, they should gain an advantage via other means, of which resource allocation is an important component. Thus, to study their sex allocation is very helpful to understand how the dimorphic sexual system is maintained in natural systems. We studied the sex allocation patterns and reproductive output of the gynodioecious Cyananthus delavayi in three populations with different soil qualities (organic matter, N, P and K). The hermaphroditic flowers and pistils were much larger than those of female individuals. Although both gender morphs invested similar biomass in the pistils, females allocated more of their resource pool to the seed production, while hermaphrodites allocated more to pollinator advertisement. The pollen production of hermaphrodites did not differ between populations, suggesting that pollen production by hermaphrodites was not limited by soil nutrients. Fruit set of females, but not hermaphrodites, decreased with declining soil quality, whereas seeds per fruit of both females and hermaphrodites were highest in poor soils. Overall, this study shows that females achieve greater reproductive success by allocating more of their resource pool to enhancing seed production, which should favor their presence in gynodioecious populations. The hermaphrodites achieve reproductive success from both pollen and seed production, and unnecessarily reduce their allocation to pollen production. Soil quality should explain, at least partially, the sexual allocation patterns. Furthermore, some of our findings contradict previous hypotheses, thus adding a new example to the body of research on plant sex allocation and the development of future theories.
[Mh] Termos MeSH primário: Campanulaceae/fisiologia
Solo/química
[Mh] Termos MeSH secundário: Biomassa
Flores/fisiologia
Pólen/fisiologia
Reprodução
Processos de Determinação Sexual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1007/s00497-017-0303-4


  3 / 176 MEDLINE  
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[PMID]:28434099
[Au] Autor:Seo JK; Kwak HR; Kim MK; Kim JS; Choi HS
[Ad] Endereço:Department of International Agricultural Technology, Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea. jangseo@snu.ac.kr.
[Ti] Título:The complete genome sequence of a novel virus, bellflower veinal mottle virus, suggests the existence of a new genus within the family Potyviridae.
[So] Source:Arch Virol;162(8):2457-2461, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A new virus was isolated from a bellflower (Campanula takesimana) plant showing veinal mottle symptoms, and its complete genome sequence was determined. The viral genome consists of a positive-sense single-stranded RNA of 8,259 ribonucleotides. Electron microscopic observation revealed that the viral genome is packaged as a filamentous particle with an average length of approximately 760 nm. BLAST searches of protein databases showed that the encoded polyprotein has a maximum amino acid sequence identity of 34.1% (with 95% coverage) to that of the isolate AD of Chinese yam necrotic mosaic virus (CYNMV; genus Macluravirus). Phylogenetic analysis and comparison of the encoded amino acid sequences with those of other viruses demonstrated that the identified virus shows minimal sequence similarity to known viruses and should therefore be considered a member of a new genus in the family Potyviridae. The name bellflower veinal mottle virus (BVMoV) is proposed for this new virus.
[Mh] Termos MeSH primário: Campanulaceae/virologia
Genoma Viral
Vírus de Plantas/genética
Vírus de Plantas/isolamento & purificação
Potyviridae/genética
Potyviridae/isolamento & purificação
[Mh] Termos MeSH secundário: Microscopia Eletrônica de Transmissão
Filogenia
Doenças das Plantas/virologia
Folhas de Planta/virologia
Vírus de Plantas/classificação
Vírus de Plantas/ultraestrutura
Potyviridae/classificação
Potyviridae/ultraestrutura
RNA Viral/genética
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170424
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3374-5


  4 / 176 MEDLINE  
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[PMID]:28186341
[Au] Autor:Crowl AA; Myers C; Cellinese N
[Ad] Endereço:Florida Museum of Natural History, University of Florida, Gainesville, Florida, 32611.
[Ti] Título:Embracing discordance: Phylogenomic analyses provide evidence for allopolyploidy leading to cryptic diversity in a Mediterranean Campanula (Campanulaceae) clade.
[So] Source:Evolution;71(4):913-922, 2017 Apr.
[Is] ISSN:1558-5646
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Mediterranean Basin harbors a remarkable amount of biodiversity, a high proportion of which is endemic to this region. Here, we present an in-depth study of an angiosperm species complex, in which cryptic taxonomic diversity has been hypothesized. Specifically, we focus on four currently recognized species in the Roucela complex, a well-supported clade in the Campanulaceae/Campanuloideae: Campanula creutzburgii, C. drabifolia, C. erinus, and C. simulans. This study takes a phylogenomic approach, utilizing near-complete plastomes and 130 nuclear loci, to uncover cryptic diversity and test hypotheses regarding hybridization and polyploidy within this clade. Genome size estimates recovered tetraploid and octoploid lineages within the currently recognized, widespread species C. erinus, showing an east-west geographic pattern. Though genomic data clearly differentiate these two cytotypes, we failed to discern morphological differences. The formation of a cryptic octoploid lineage, distributed across the eastern Mediterranean, is hypothesized to be the result of an allopolyploid event in which one parental morphology is retained. The tetraploid C. erinus and C. creutzburgii (also a tetraploid) are implicated as parental lineages. Our results highlight the utility of target-enrichment approaches for obtaining genomic datasets for thorough assessments of species diversity and the importance of carefully considering gene-tree discordance within such datasets.
[Mh] Termos MeSH primário: Biodiversidade
Campanulaceae/classificação
Campanulaceae/genética
Filogenia
Poliploidia
[Mh] Termos MeSH secundário: Genoma de Planta
Hibridização Genética
Região do Mediterrâneo
Filogeografia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1111/evo.13203


  5 / 176 MEDLINE  
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[PMID]:28032267
[Au] Autor:Brandt K; Dötterl S; Francke W; Ayasse M; Milet-Pinheiro P
[Ad] Endereço:Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Ulm, Germany.
[Ti] Título:Flower Visitors of Campanula: Are Oligoleges More Sensitive to Host-Specific Floral Scents Than Polyleges?
[So] Source:J Chem Ecol;43(1):4-12, 2017 Jan.
[Is] ISSN:1573-1561
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The pollen diet provided by adult bees to their offspring varies immensely. While some species collect pollen on several plants irrespective of their phylogenetic relatedness (polyleges), others collect only on plants within a genus or family (oligoleges). Floral scents play a central role in bee-plant interactions. To locate flowers, polyleges are assumed to rely on compounds commonly found as floral scent constituents, whereas oligoleges rely on unusual compounds to recognize host flowers unambiguously. Campanula flowers are visited by both polylectic and oligolectic species, and their scent bouquets consist of common and unusual (e.g., spiroacetals) volatiles. In a comparative approach, we performed electroantennographic analyses to investigate the antennal responses of three polyleges and three oligoleges to three common volatiles and four spiroacetals. We hypothesized that: 1) oligoleges and polyleges should respond similarly to common flower volatiles, and 2) Campanula oligoleges should be more sensitive to spiroacetals than are polyleges. In corroboration, we found that antennal sensitivity to common volatiles was similar among bees irrespective of pollen diet, whereas oligoleges of Campanula were more sensitive to spiroacetals than polyleges. Newly emerged bees of the Campanula oligolege Chelostoma rapunculi rely on spiroacetals for recognizing host-flowers, and our results suggest that this might also be true for other Campanula oligoleges, since Chelostoma campanularum and Hoplitis mitis also were able to perceive these specific volatiles at very low concentrations. Together, our results provide interesting insights into the significance of olfactory adaptations in oligolectic and polylectic bee species.
[Mh] Termos MeSH primário: Abelhas/fisiologia
Campanulaceae
Flores/química
Odorantes/análise
Polinização
[Mh] Termos MeSH secundário: Acetais/análise
Animais
Antenas de Artrópodes/fisiologia
Fenômenos Eletrofisiológicos
Feminino
Especificidade da Espécie
Compostos de Espiro/análise
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetals); 0 (Spiro Compounds)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161230
[St] Status:MEDLINE
[do] DOI:10.1007/s10886-016-0802-z


  6 / 176 MEDLINE  
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[PMID]:28011338
[Au] Autor:Uribe-Convers S; Carlsen MM; Lagomarsino LP; Muchhala N
[Ad] Endereço:University of Missouri-St. Louis, Biology Department, One University Blvd., Research Building, St. Louis, MO 63121, USA. Electronic address: uribe.convers@gmail.com.
[Ti] Título:Phylogenetic relationships of Burmeistera (Campanulaceae: Lobelioideae): Combining whole plastome with targeted loci data in a recent radiation.
[So] Source:Mol Phylogenet Evol;107:551-563, 2017 Feb.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The field of molecular systematics has benefited greatly with the advent of high-throughput sequencing (HTS), making large genomic datasets commonplace. However, a large number of targeted Sanger sequences produced by many studies over the last two decades are publicly available and should not be overlooked. In this study, we elucidate the phylogenetic relationships of the plant genus Burmeistera (Campanulaceae: Lobelioideae), while investigating how to best combine targeted Sanger loci with HTS data. We sequence, annotate, and analyze complete to nearly complete plastomes for a subset of the genus. We then combine these data with a much larger taxonomic dataset for which only Sanger sequences are available, making this the most comprehensively sampled study in the genus to date. We show that using a phylogeny inferred from the species with plastome data as a topological constraint for the larger dataset increases the resolution of our data and produces a more robust evolutionary hypothesis for the group. We then use the resulting phylogeny to study the evolution of morphological traits thought to be important in Burmeistera, and assess their usefulness in the current taxonomic classification of the genus. The main morphological character used to delimit subgeneric sections, the presence or absence of hairs on the apex of the two ventral anthers, shows a complex evolutionary history with many changes in the tree, suggesting that this character should not be used for taxonomic classification. Although it is too soon to propose a new subgeneric classification for Burmeistera, our results highlight some morphological traits shared by whole clades that could potentially be used in future taxonomic work.
[Mh] Termos MeSH primário: Campanulaceae/classificação
Campanulaceae/genética
Loci Gênicos
Genomas de Plastídeos
Filogenia
[Mh] Termos MeSH secundário: Sequência de Bases
Sequenciamento de Nucleotídeos em Larga Escala
Fenótipo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161225
[St] Status:MEDLINE


  7 / 176 MEDLINE  
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[PMID]:27677969
[Au] Autor:Barnard-Kubow KB; So N; Galloway LF
[Ad] Endereço:Department of Biology, University of Virginia, Charlottesville, Virginia, 22904.
[Ti] Título:Cytonuclear incompatibility contributes to the early stages of speciation.
[So] Source:Evolution;70(12):2752-2766, 2016 Dec.
[Is] ISSN:1558-5646
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Genetic incompatibility is a hallmark of speciation. Cytonuclear incompatibilities are proposed to be among the first genetic barriers to arise during speciation. Accordingly, reproductive isolation (RI) within species should be heavily influenced by interactions between the organelle and nuclear genomes. However, there are few clear examples of cytonuclear incompatibility within a species. Here, we show substantial postzygotic RI in first-generation hybrids between differentiated populations of an herbaceous plant (up to 92% reduction in fitness). RI was primarily due to germination and survival, with moderate RI for pollen viability. RI for survival was asymmetric and caused by cytonuclear incompatibility, with the strength of incompatibility linearly related to chloroplast genetic distance. This cytonuclear incompatibility may be the result of a rapidly evolving plastid genome. Substantial asymmetric RI was also found for germination, but was not associated with cytonuclear incompatibility, indicating endosperm or maternal-zygote incompatibilities. These results demonstrate that cytonuclear incompatibility contributes to RI within species, suggesting that initial rates of speciation could be influenced by rates of organelle evolution. However, other genetic incompatibilities are equally important, indicating that even at early stages, speciation can be a complex process involving multiple genes and incompatibilities.
[Mh] Termos MeSH primário: Campanulaceae/fisiologia
Especiação Genética
Isolamento Reprodutivo
[Mh] Termos MeSH secundário: Campanulaceae/genética
Geografia
Hibridização Genética
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE
[do] DOI:10.1111/evo.13075


  8 / 176 MEDLINE  
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[PMID]:27478100
[Au] Autor:Li JW; Liu Y; Li BH; Wang YY; Wang H; Zhou CL
[Ad] Endereço:State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
[Ti] Título:A polysaccharide purified from Radix Adenophorae promotes cell activation and pro-inflammatory cytokine production in murine RAW264.7 macrophages.
[So] Source:Chin J Nat Med;14(5):370-6, 2016 May.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Radix Adenophorae, a traditional Chinese medicine, has been reported to have a variety of biological functions. In the present study, a polysaccharide component, Radix Adenophorae Polysaccharide (RAPS), was purified from Radix Adenophorae by decoloring with ADS-7 macroporous adsorption resin, DEAE-52 cellulose ion-exchange chromatography, and Sephacryl S-300HR gel chromatography, with the purity of 98.3% and a molecular weight of 1.8 × 10(4) Da. The cell viability assay and microscopic examination revealed that RAPS promoted the proliferation and activation of macrophages. At 400 µg·mL(-1), RAPS stimulated RAW264.7 cell proliferation by 1.91-fold compared with the control. Meanwhile, RAPS significantly increased the secretion of pro-inflammatory cytokines (TNF-α and IL-6) in a dose-dependent manner in the supernatant of RAW264.7 cell culture as determined by ELISA. At 400 µg·mL(-1), the production of TNF-iα was 20.8-fold higher than that of the control. Simultaneously, the production of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were increased in RAW264.7 cells incubated with RAPS, as measured by Griess assay and Western blot analysis. The NO production of cells treated with RAPS (400 µg·mL(-1)) reached 15.8 µmol·L(-1), which was 30.4-fold higher than that of the control (0.53 µmol·L(-1)). These data suggested that RAPS may enhance the immune function and protect against exogenous pathogens by activating macrophages.
[Mh] Termos MeSH primário: Campanulaceae/química
Citocinas/imunologia
Fatores Imunológicos/farmacologia
Ativação de Macrófagos/efeitos dos fármacos
Macrófagos/efeitos dos fármacos
Extratos Vegetais/farmacologia
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Citocinas/genética
Interleucina-6/genética
Interleucina-6/imunologia
Macrófagos/imunologia
Camundongos
Óxido Nítrico/imunologia
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Immunologic Factors); 0 (Interleukin-6); 0 (Plant Extracts); 0 (Polysaccharides); 0 (Tumor Necrosis Factor-alpha); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160802
[St] Status:MEDLINE


  9 / 176 MEDLINE  
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[PMID]:27215645
[Au] Autor:Jensen L; Hegelund JN; Olsen A; Lütken H; Müller R
[Ad] Endereço:Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Højbakkegård Allé 9-13, 2630, Taastrup, Denmark.
[Ti] Título:A natural frameshift mutation in Campanula EIL2 correlates with ethylene insensitivity in flowers.
[So] Source:BMC Plant Biol;16(1):117, 2016 May 23.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The phytohormone ethylene plays a central role in development and senescence of climacteric flowers. In ornamental plant production, ethylene sensitive plants are usually protected against negative effects of ethylene by application of chemical inhibitors. In Campanula, flowers are sensitive to even minute concentrations of ethylene. RESULTS: Monitoring flower longevity in three Campanula species revealed C. portenschlagiana (Cp) as ethylene sensitive, C. formanekiana (Cf) with intermediate sensitivity and C. medium (Cm) as ethylene insensitive. We identified key elements in ethylene signal transduction, specifically in Ethylene Response Sensor 2 (ERS2), Constitutive Triple Response 1 (CTR1) and Ethylene Insensitive 3- Like 1 and 2 (EIL1 and EIL2) homologous. Transcripts of ERS2, CTR1 and EIL1 were constitutively expressed in all species both throughout flower development and in response to ethylene. In contrast, EIL2 was found only in Cf and Cm. We identified a natural mutation in Cmeil2 causing a frameshift which resulted in difference in expression levels of EIL2, with more than 100-fold change between Cf and Cm in young flowers. CONCLUSIONS: This study shows that the naturally occurring 7 bp frameshift discovered in Cmeil2, a key gene in the ethylene signaling pathway, correlates with ethylene insensitivity in flowers. We suggest that transfer of the eil2 mutation to other plant species will provide a novel tool to engineer ethylene insensitive flowers.
[Mh] Termos MeSH primário: Campanulaceae/genética
Etilenos/metabolismo
Mutação da Fase de Leitura
Regulação da Expressão Gênica de Plantas
Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Campanulaceae/metabolismo
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Alinhamento de Sequência
Especificidade da Espécie
Fatores de Transcrição/química
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenes); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (Transcription Factors); 91GW059KN7 (ethylene)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160525
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-016-0786-4


  10 / 176 MEDLINE  
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[PMID]:27208642
[Au] Autor:Stuchlíková L; Jirásko R; Skálová L; Pavlík F; Szotáková B; Holcapek M; Vanek T; Podlipná R
[Ad] Endereço:Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
[Ti] Título:Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia).
[So] Source:Chemosphere;157:10-7, 2016 Aug.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Benzimidazoles anthelmintics, which enter into environment primarily through excretion in the feces or urine of treated animals, can affect various organisms and disrupt ecosystem balance. The present study was designed to test the phytotoxicity and biotransformation of the three benzimidazole anthelmintics albendazole (ABZ), fenbendazole (FBZ) and flubendazole (FLU) in the harebell (Campanula rotundifolia). This meadow plant commonly grows in pastures and comes into contact with anthelmintics through the excrements of treated animals. Suspensions of harebell cells in culture medium were used as an in vitro model system. ABZ, FLU and FBZ were not found to be toxic for harebell cells, which were able to metabolize ABZ, FLU and FBZ via the formation of a wide scale of metabolites. Ultrahigh-performance liquid chromatography coupled with high mass accuracy tandem mass spectrometry (UHPLC-MS/MS) led to the identification of 24, 18 and 29 metabolites of ABZ, FLU and FBZ, respectively. Several novel metabolites were identified for the first time. Based on the obtained results, the schemes of the metabolic pathways of these anthelmintics were proposed. Most of these metabolites can be considered deactivation products, but a substantial portion of them may readily be decomposed to biologically active substances which could negatively affect ecosystems.
[Mh] Termos MeSH primário: Anti-Helmínticos/metabolismo
Benzimidazóis/metabolismo
Campanulaceae/metabolismo
Redes e Vias Metabólicas
[Mh] Termos MeSH secundário: Albendazol/metabolismo
Animais
Anti-Helmínticos/química
Benzimidazóis/química
Biotransformação
Campanulaceae/citologia
Células Cultivadas
Ecossistema
Fezes/química
Fenbendazol/metabolismo
Mebendazol/análogos & derivados
Mebendazol/metabolismo
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Benzimidazoles); 621BVT9M36 (Fenbendazole); 81G6I5V05I (Mebendazole); F4216019LN (Albendazole); R8M46911LR (flubendazole)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE



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