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Pesquisa : B01.650.940.800.575.912.250.171.600 [Categoria DeCS]
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[PMID]:28645921
[Au] Autor:Knox EB; Li C
[Ad] Endereço:Department of Biology, Indiana University, Bloomington, Indiana 47405 USA eknox@indiana.edu.
[Ti] Título:The East Asian origin of the giant lobelias.
[So] Source:Am J Bot;104(6):924-938, 2017 Jun.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Rapid radiations are difficult to reconstruct when organismal diversification and biogeographic movement outpace the evolution of genes typically used in phylogenetic analyses. The 125 kb of unique sequence from complete plastid genomes (= plastomes) largely solves the molecular sampling problem, and taxon sampling that triangulates the base of each major subclade largely solves the long-branch attraction problem. This combination of molecular and phylogenetic sampling is used to reconstruct the cosmopolitan radiation of lobeliads, with special focus on the origin of the giant lobelias. METHODS: An alignment of 18 previously generated and 61 new plastomes was analyzed to produce the phylogenetic estimate upon which the biogeographic reconstruction was based. KEY RESULTS: Originating in southern Africa, the Lobeliaceae underwent a spectacular cosmopolitan radiation about 20 million years ago. One lineage colonized Madagascar and eastern Asia, which was the source area for the evolution of the giant lobelias. A second lineage colonized the Mediterranean and North America, in quick succession. South America and Australia were also colonized from South Africa, most likely as independent events, but detailed biogeographic reconstruction is limited by inferred extinction events. The south Pacific segregate genera and are inferred to have Hawaiian ancestry. The East African radiation independently reached Ethiopia, West Africa, and Brazil. CONCLUSIONS: With adequate molecular and taxon sampling, many details of rapid radiations can be accurately inferred. However, not all lineages survived, and analyses of extant species cannot recover details that have been lost due to extinction.
[Mh] Termos MeSH primário: Evolução Biológica
Lobelia/classificação
Filogenia
[Mh] Termos MeSH secundário: Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1700025


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[PMID]:28325832
[Au] Autor:Bailey MF; Case AL; Caruso CM
[Ad] Endereço:Department of Biology, Providence College, Providence, Rhode Island 02918, USA mbailey9@providence.edu.
[Ti] Título:Physiological effects of temperature do not explain prevalence of females in populations of gynodioecious growing in warmer climates.
[So] Source:Am J Bot;104(3):411-418, 2017 Mar.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Gynodioecy is a sexual polymorphism whereby female and hermaphroditic plants co-occur within populations. In many gynodioecious species, stressful abiotic environments are associated with higher frequencies of females. This association suggests that abiotic stress affects the relative fitness of females and hermaphrodites and, thus, the maintenance of gynodioecy. METHODS: To test whether abiotic stress affects the fitness of females and hermaphrodites, we grew open-pollinated families in temperature regimes characteristic of the southern portion of the species' range (where females are common) and the northern portion of the range (where females are rare). We measured physiological and phenological traits that are indicative of heat stress, and fitness components of females and hermaphrodites that could affect the maintenance of gynodioecy. KEY RESULTS: Contrary to expectations if growth at high temperatures is stressful, we found that the hot treatment increased leaf chlorophyll content, decreased the percentage of plants that delayed flowering initiation, and did not affect the quantum efficiency of photosystem II. Growth at high temperatures did not affect the magnitude of the difference in rosette size (a correlate of flower number) between females and hermaphrodites, or the variance in pollen viability among hermaphrodites. CONCLUSIONS: We found that growing-season temperatures typical of high female . populations were not stressful and did not affect either the fitness of females compared to hermaphrodites or variation in fitness among hermaphrodites. Consequently, further research is necessary to explain correlations between abiotic environmental factors and the frequency of females in this and other gynodioecious species.
[Mh] Termos MeSH primário: Lobelia/fisiologia
[Mh] Termos MeSH secundário: Clorofila/metabolismo
Clima
Flores/crescimento & desenvolvimento
Flores/fisiologia
Lobelia/crescimento & desenvolvimento
Fenótipo
Pólen/crescimento & desenvolvimento
Pólen/fisiologia
Polinização
Reprodução
Razão de Masculinidade
Estresse Fisiológico
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
1406-65-1 (Chlorophyll)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600380


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[PMID]:27696560
[Au] Autor:Hsu YH; Tagami T; Matsunaga K; Okuyama M; Suzuki T; Noda N; Suzuki M; Shimura H
[Ad] Endereço:Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
[Ti] Título:Functional characterization of UDP-rhamnose-dependent rhamnosyltransferase involved in anthocyanin modification, a key enzyme determining blue coloration in Lobelia erinus.
[So] Source:Plant J;89(2):325-337, 2017 Jan.
[Is] ISSN:1365-313X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Because structural modifications of flavonoids are closely related to their properties, such as stability, solubility, flavor and coloration, characterizing the enzymes that catalyze the modification reactions can be useful for engineering agriculturally beneficial traits of flavonoids. In this work, we examined the enzymes involved in the modification pathway of highly glycosylated and acylated anthocyanins that accumulate in Lobelia erinus. Cultivar Aqua Blue (AB) of L. erinus is blue-flowered and accumulates delphinidin 3-O-p-coumaroylrutinoside-5-O-malonylglucoside-3'5'-O-dihydroxycinnamoylglucoside (lobelinins) in its petals. Cultivar Aqua Lavender (AL) is mauve-flowered, and LC-MS analyses showed that AL accumulated delphinidin 3-O-glucoside (Dp3G), which was not further modified toward lobelinins. A crude protein assay showed that modification processes of lobelinin were carried out in a specific order, and there was no difference between AB and AL in modification reactions after rhamnosylation of Dp3G, indicating that the lack of highly modified anthocyanins in AL resulted from a single mutation of rhamnosyltransferase catalyzing the rhamnosylation of Dp3G. We cloned rhamnosyltransferase genes (RTs) from AB and confirmed their UDP-rhamnose-dependent rhamnosyltransferase activities on Dp3G using recombinant proteins. In contrast, the RT gene in AL had a 5-bp nucleotide deletion, resulting in a truncated polypeptide without the plant secondary product glycosyltransferase box. In a complementation test, AL that was transformed with the RT gene from AB produced blue flowers. These results suggest that rhamnosylation is an essential process for lobelinin synthesis, and thus the expression of RT has a great impact on the flower color and is necessary for the blue color of Lobelia flowers.
[Mh] Termos MeSH primário: Antocianinas/metabolismo
Lobelia/fisiologia
Proteínas de Plantas/metabolismo
Açúcares de Uridina Difosfato/metabolismo
[Mh] Termos MeSH secundário: Clonagem Molecular
Teste de Complementação Genética
Glucosídeos/metabolismo
Hexosiltransferases/genética
Hexosiltransferases/metabolismo
Lobelia/genética
Lobelia/metabolismo
Filogenia
Pigmentação
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Glucosides); 0 (Plant Proteins); 0 (Recombinant Proteins); 0 (Uridine Diphosphate Sugars); 0 (delphinidin 3-O-glucopyranoside); 1955-26-6 (UDP-rhamnose); EC 2.4.1.- (Hexosyltransferases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE
[do] DOI:10.1111/tpj.13387


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[PMID]:28487901
[Au] Autor:Cui X; Gu X; Kang W
[Ad] Endereço:Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China.
[Ti] Título:ANTIOXIDANT ACTIVITY AND HEPATOPROTECTIVE EFFECTS OF COMPOUND .
[So] Source:Afr J Tradit Complement Altern Med;13(5):114-122, 2016.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Chinese medicine has its own uniqueness, advantageous in the treatment of hepatic diseases, and they were widely used in the oxidation. At the same time, oxidation is one of the mechanism of protect liver. MATERIALS AND METHODS: In the present study, the antioxidant activity of different extracts from Compound Lobelia were estimated respectively by the methods of measuring the [2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid] diamonium salt (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP). The protective effects on carbon tetrachloride (CCl4)-induced acute liver injury in mice which was investigated by analyzing the result of biochemical parameters such as glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in serum, superoxide dismutase (SOD) and malondialdehyde (MDA) in liver tissue homogenate. RESULT: the result showed that ABTS free radical scavenging activity of ethanol extract (IC =29.26±0.49 µg/mL) was stronger than that of water extract (IC50=42.09±2.44 µg/mL), but they were lower than that of BHT as positive control (IC50=2.47±0.09 µg/mL). The ferric reducing antioxidant power of ethanol extract (FRAP=329.03±46.30 µmol/g) was higher than that of water extract (FRAP=206.03±54.30 µmol/g); they were lower than that of BHT (FRAP=1541.87±9.70 µmol/g). Water extract and ethanol extract could significantly reduce GOT activity ( <0.001) and GPT activity ( <0.001) in serum, reduce significantly the content of MDA ( <0.001) and significantly increase SOD activity ( <0.001) in the mice liver tissue homogenate. CONCLUSION: Compound lobelia had a certain antioxidant and satisfied hepatoprotective effects. Moreover, the liver-protective effect was concerned with antioxidant activity and enhances the body immunity. Compound lobelia is expected to be a new medicine for protecting liver.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Medicamentos de Ervas Chinesas/farmacologia
Lobelia/química
Fitoterapia/métodos
Substâncias Protetoras/farmacologia
[Mh] Termos MeSH secundário: Alanina Transaminase/sangue
Animais
Aspartato Aminotransferases/sangue
Benzotiazóis
Tetracloreto de Carbono
Doença Hepática Induzida por Substâncias e Drogas/sangue
Doença Hepática Induzida por Substâncias e Drogas/etiologia
Fígado/efeitos dos fármacos
Masculino
Malondialdeído/análise
Camundongos
Oxirredução/efeitos dos fármacos
Ácidos Sulfônicos
Superóxido Dismutase/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Benzothiazoles); 0 (Drugs, Chinese Herbal); 0 (Protective Agents); 0 (Sulfonic Acids); 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid); 4Y8F71G49Q (Malondialdehyde); CL2T97X0V0 (Carbon Tetrachloride); EC 1.15.1.1 (Superoxide Dismutase); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170812
[Lr] Data última revisão:
170812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v13i5.15


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[PMID]:27843296
[Au] Autor:Ling B; Michel D; Sakharkar MK; Yang J
[Ad] Endereço:Drug Discovery and Development Research Group, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK; Department of Biomedical and Molecular Sciences, Queen's University, Cancer Biology & Genetics Division, Queen's Cancer Research Institute, Kingston, ON, Canada.
[Ti] Título:Evaluating the cytotoxic effects of the water extracts of four anticancer herbs against human malignant melanoma cells.
[So] Source:Drug Des Devel Ther;10:3563-3572, 2016.
[Is] ISSN:1177-8881
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Malignant melanoma (MM) is the most dangerous type of skin cancer, killing more than 1,100 people each year in Canada. Prognosis for late stage and recurrent MM is extremely poor due to insensitivity to chemotherapy drugs, and thus many patients seek complementary and alternative medicines. In this study, we examined four commonly used anticancer herbs in traditional Chinese medicine, , , , and , for their in vitro antitumor effects toward human MM cell line A-375. The crude water extract of (1 g of dry herb in 100 mL water) and its 2-fold dilution caused 52.8%±13.0% and 17.3%±2.7% cytotoxicity in A-375 cells, respectively ( <0.01). The crude water extract of caused 11.1%±12.4% cytotoxicity in A-375 cells with no statistical significance ( >0.05). Higher concentrated formulation might be needed for to exert its cytotoxic effect against A-375 cells. No cytotoxicity was observed in A-375 cells treated with crude water extract of and . Further high performance liquid chromatography-tandem mass spectroscopy analysis of the herbal extracts implicated that and might have adopted the same bioactive components for their cytotoxic effects in spite of belonging to two different plant families. We also showed that the crude water extract of reduced intracellular reactive oxygen species generation in A-375 cells, which may lead to a cytostatic effect. Furthermore, synergistic effect was achieved when crude water extract of was coadministered with temozolomide, a chemotherapy drug for skin cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Hedyotis
Lobelia
Melanoma/tratamento farmacológico
Extratos Vegetais/farmacologia
Scutellaria
Neoplasias Cutâneas/tratamento farmacológico
Solanum nigrum
Solventes/química
Água/química
[Mh] Termos MeSH secundário: Antineoplásicos Alquilantes/farmacologia
Antineoplásicos Fitogênicos/isolamento & purificação
Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Linhagem Celular Tumoral
Cromatografia Líquida de Alta Pressão
Dacarbazina/análogos & derivados
Dacarbazina/farmacologia
Relação Dose-Resposta a Droga
Sinergismo Farmacológico
Hedyotis/química
Seres Humanos
Lobelia/química
Melanoma/metabolismo
Melanoma/patologia
Fitoterapia
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Espécies Reativas de Oxigênio/metabolismo
Scutellaria/química
Neoplasias Cutâneas/metabolismo
Neoplasias Cutâneas/patologia
Solanum nigrum/química
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Alkylating); 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Plant Extracts); 0 (Reactive Oxygen Species); 0 (Solvents); 059QF0KO0R (Water); 7GR28W0FJI (Dacarbazine); YF1K15M17Y (temozolomide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE


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[PMID]:27637316
[Au] Autor:Brown DP; Rogers DT; Gunjan SK; Gerhardt GA; Littleton JM
[Ad] Endereço:Department of Anatomy & Neurobiology, University of Kentucky, Lexington, KY, 40536-0298, USA.
[Ti] Título:Target-directed discovery and production of pharmaceuticals in transgenic mutant plant cells.
[So] Source:J Biotechnol;238:9-14, 2016 Nov 20.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Plants are a source of complex bioactive compounds, with value as pharmaceuticals, or leads for synthetic modification. Many of these secondary metabolites have evolved as defenses against competing organisms and their pharmaceutical value is "accidental", resulting from homology between target proteins in these competitors, and human molecular therapeutic targets. Here we show that it is possible to use mutation and selection of plant cells to re-direct their "evolution" toward metabolites that interact with the therapeutic target proteins themselves. This is achieved by expressing the human target protein in plant cells, and selecting mutants for survival based on the interaction of their metabolome with this target. This report describes the successful evolution of hairy root cultures of a Lobelia species toward increased biosynthesis of metabolites that inhibit the human dopamine transporter protein. Many of the resulting selected mutants are overproducing the active metabolite found in the wild-type plant, but others overproduce active metabolites that are not readily detectable in non-mutants. This technology can access the whole genomic capability of a plant species to biosynthesize metabolites with a specific target. It has potential value as a novel platform for plant drug discovery and production, or as a means of optimizing the therapeutic value of medicinal plant extracts.
[Mh] Termos MeSH primário: Lobelia
Células Vegetais/metabolismo
Plantas Geneticamente Modificadas
Engenharia de Proteínas/métodos
Proteínas Recombinantes
[Mh] Termos MeSH secundário: Proteínas da Membrana Plasmática de Transporte de Dopamina/genética
Proteínas da Membrana Plasmática de Transporte de Dopamina/metabolismo
Seres Humanos
Lobelia/citologia
Lobelia/genética
Lobelia/metabolismo
Raízes de Plantas
Plantas Geneticamente Modificadas/citologia
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Técnicas de Cultura de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dopamine Plasma Membrane Transport Proteins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160918
[St] Status:MEDLINE


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[PMID]:27314319
[Au] Autor:Li XJ; Bao WR; Leung CH; Ma DL; Zhang G; Lu AP; Wang SC; Han QB
[Ad] Endereço:Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. lixiaojun@hkbu.edu.hk.
[Ti] Título:Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour.
[So] Source:Molecules;21(6), 2016 Jun 15.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A neutral α-glucan, named BP1, with a molecular mass of approximately 9.45 kDa, was isolated from Lobelia chinensis by hot-water extraction, a Q-Sepharose Fast Flow column and Superdex-75 column chromatography. Its chemical structure was characterized by monosaccharide analysis, methylation analysis and analysis of its FT-IR, high performance gel permeation chromatography (HPGPC) and 1D/2D-NMR spectra data. The backbone of BP1 consists of →6α-d-Glcp¹â†’6,3α-d-Glcp¹â†’(6α-d-Glcp¹)x-6,3α-d-Glcp¹-(6α-d-Glcp¹)y→. The side chains were terminal α-d-Glcp¹â†’ and α-d-Glcp¹â†’ (6α-d-Glcp¹)z→4α-d-Glcp¹â†’3α-d-Glcp¹â†’4α-d-Glcp¹â†’ (x + y + z = 5), which are attached to the backbone at O-3 of 3,6α-d-Glcp¹. The results of the effect of BP1 on mouse macrophage cell line RAW 264.7 indicate that BP1 enhances the cell proliferation, phagocytosis, nitric oxide production and cytokine secretion in a dose-dependent manner. Because the inhibitor of Toll-like receptor 4 blocks the BP1-induced secretion of TNF-α and IL-6, we hypothesize that α-glucan BP1 activates TLR4, which mediates the above-mentioned immunomodulating effects.
[Mh] Termos MeSH primário: Glucanos/química
Interleucina-6/biossíntese
Lobelia/química
Receptor 4 Toll-Like/biossíntese
Fator de Necrose Tumoral alfa/biossíntese
[Mh] Termos MeSH secundário: Animais
Regulação da Expressão Gênica/efeitos dos fármacos
Glucanos/administração & dosagem
Glucanos/isolamento & purificação
Imunomodulação
Metilação
Camundongos
Monossacarídeos/química
Monossacarídeos/isolamento & purificação
Fagocitose/efeitos dos fármacos
Células RAW 264.7/efeitos dos fármacos
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (Interleukin-6); 0 (Monosaccharides); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 4); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160618
[St] Status:MEDLINE


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[PMID]:27246225
[Au] Autor:Moora M; Öpik M; Davison J; Jairus T; Vasar M; Zobel M; Eckstein RL
[Ad] Endereço:Department of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Lai 40, 51005, Tartu, Estonia.
[Ti] Título:AM fungal communities inhabiting the roots of submerged aquatic plant Lobelia dortmanna are diverse and include a high proportion of novel taxa.
[So] Source:Mycorrhiza;26(7):735-45, 2016 Oct.
[Is] ISSN:1432-1890
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:While the arbuscular mycorrhizal (AM) symbiosis is known to be widespread in terrestrial ecosystems, there is growing evidence that aquatic plants also form the symbiosis. It has been suggested that symbiosis with AM fungi may represent an important adaptation for isoëtid plants growing on nutrient-poor sediments in oligotrophic lakes. In this study, we address AM fungal root colonization intensity, richness and community composition (based on small subunit (SSU) ribosomal RNA (rRNA) gene sequencing) in five populations of the isoëtid plant species Lobelia dortmanna inhabiting oligotrophic lakes in Southern Sweden. We found that the roots of L. dortmanna hosted rich AM fungal communities and about 15 % of the detected molecular taxa were previously unrecorded. AM fungal root colonization intensity and taxon richness varied along an environmental gradient, being higher in oligotrophic and lower in mesotrophic lakes. The overall phylogenetic structure of this aquatic fungal community differed from that described in terrestrial systems: The roots of L. dortmanna hosted more Archaeosporaceae and fewer Glomeraceae taxa than would be expected based on global data from terrestrial AM fungal communities.
[Mh] Termos MeSH primário: Fungos/genética
Lobelia/microbiologia
Micorrizas/classificação
Micorrizas/fisiologia
Raízes de Plantas/microbiologia
[Mh] Termos MeSH secundário: Ecossistema
Fungos/classificação
Filogenia
RNA Fúngico/genética
RNA Ribossômico/genética
Suécia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Fungal); 0 (RNA, Ribosomal)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160602
[St] Status:MEDLINE
[do] DOI:10.1007/s00572-016-0709-0


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[PMID]:27105955
[Au] Autor:Brown DP; Rogers DT; Pomerleau F; Siripurapu KB; Kulshrestha M; Gerhardt GA; Littleton JM
[Ad] Endereço:College of Medicine, Department of Anatomy & Neurobiology, University of Kentucky Chandler Medical Center, 138 Leader Avenue, Lexington, KY 40536-9983, USA.
[Ti] Título:Novel multifunctional pharmacology of lobinaline, the major alkaloid from Lobelia cardinalis.
[So] Source:Fitoterapia;111:109-23, 2016 Jun.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In screening a library of plant extracts from ~1000 species native to the Southeastern United States, Lobelia cardinalis was identified as containing nicotinic acetylcholine receptor (nicAchR) binding activity which was relatively non-selective for the α4ß2- and α7-nicAchR subtypes. This nicAchR binding profile is atypical for plant-derived nicAchR ligands, the majority of which are highly selective for α4ß2-nicAchRs. Its potential therapeutic relevance is noteworthy since agonism of α4ß2- and α7-nicAchRs is associated with anti-inflammatory and neuroprotective properties. Bioassay-guided fractionation of L. cardinalis extracts led to the identification of lobinaline, a complex binitrogenous alkaloid, as the main source of the unique nicAchR binding profile. Purified lobinaline was a potent free radical scavenger, displayed similar binding affinity at α4ß2- and α7-nicAchRs, exhibited agonist activity at nicAchRs in SH-SY5Y cells, and inhibited [(3)H]-dopamine (DA) uptake in rat striatal synaptosomes. Lobinaline significantly increased fractional [(3)H] release from superfused rat striatal slices preloaded with [(3)H]-DA, an effect that was inhibited by the non-selective nicAchR antagonist mecamylamine. In vivo electrochemical studies in urethane-anesthetized rats demonstrated that lobinaline locally applied in the striatum significantly prolonged clearance of exogenous DA by the dopamine transporter (DAT). In contrast, lobeline, the most thoroughly investigated Lobelia alkaloid, is an α4ß2-nicAchR antagonist, a poor free radical scavenger, and is a less potent DAT inhibitor. These previously unreported multifunctional effects of lobinaline make it of interest as a lead to develop therapeutics for neuropathological disorders that involve free radical generation, cholinergic, and dopaminergic neurotransmission. These include neurodegenerative conditions, such as Parkinson's disease, and drug abuse.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Lobelia/química
Antagonistas Nicotínicos/farmacologia
Quinolinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Corpo Estriado/efeitos dos fármacos
Dopamina/metabolismo
Ensaios de Triagem em Larga Escala
Masculino
Ratos
Ratos Sprague-Dawley
Receptores Nicotínicos/metabolismo
Sinaptossomos/efeitos dos fármacos
Sinaptossomos/metabolismo
Receptor Nicotínico de Acetilcolina alfa7/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Nicotinic Antagonists); 0 (Quinolines); 0 (Receptors, Nicotinic); 0 (alpha7 Nicotinic Acetylcholine Receptor); 0 (lobinaline); 0 (nicotinic receptor alpha4beta2); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160424
[St] Status:MEDLINE


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[PMID]:26801601
[Au] Autor:Banumathi B; Malaikozhundan B; Vaseeharan B
[Ad] Endereço:Department of Animal Health and Management, Alagappa University, Science Block 4th Floor, Burma Colony, Karaikudi 630 004, Tamil Nadu, India.
[Ti] Título:Invitro acaricidal activity of ethnoveterinary plants and green synthesis of zinc oxide nanoparticles against Rhipicephalus (Boophilus) microplus.
[So] Source:Vet Parasitol;216:93-100, 2016 Jan 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study was designed to investigate the invitro acaricidal effects of seven ethnoveterinary plants, zinc acetate and green synthesized zinc oxide nanoparticles against the Southern cattle tick, Rhipicephalus (Boophilus) microplus. The selected ethnoveterinary plants were extracted using ethanol and aqueous (water) solvents at 0.02mg/ml and 0.04mg/ml concentrations. Of these seven plants, Lobelia leschenaultiana showed the highest percentage of tick mortality. The ethanol extracts of L. leschenaultiana showed 93.33% mortality at 0.04mg/ml and its LC50 was 0.05mg/ml. However, zinc acetate exhibited 70% mortality at 0.04mg/ml (LC50: 0.0192mg/ml). Further, we synthesized ZnO nanoparticle using the leaf extracts of L. leschenaultiana and zinc acetate as the precursor material to control R. (B.) microplus. The structural characterization of the synthesized ZnO nanoparticles (Ll-ZnO NPs) was performed by UV-vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and transmission electron microsopy (TEM). UV-vis spectra showed the absorption band at 383nm. XRD analysis clearly showed the crystalline nature of Ll-ZnO NPs with various Bragg's reflection peaks at 100, 002, 101, 102, 110, 103, 200, 201 and 202 planes. FTIR analysis showed the possible functional groups of Ll-ZnO NPs with strong band at 3420.63 and 2922.48cm(-1). SEM and TEM analysis revealed that the Ll-ZnO NPs were spherical and hexagonal in shape with particle size ranging between 20 and 65nm. The mortality of R. (B.) microplus after treatment with Ll-ZnO NPs was 35, 57.5 and 82.5% at 0.001, 0.002 and 0.004mg/ml. On the otherhand, 100% mortality of R. (B.) microplus was observed at 0.008mg/ml (LC50: 0.0017mg/ml). The results indicated that the Ll-ZnO NPs have good acaricidal properties compared to L. leschenaultiana leaf extract and zinc acetate.
[Mh] Termos MeSH primário: Doenças dos Bovinos/tratamento farmacológico
Lobelia/química
Fitoterapia/veterinária
Rhipicephalus
Infestações por Carrapato/veterinária
[Mh] Termos MeSH secundário: Acaricidas/farmacologia
Animais
Bovinos
Doenças dos Bovinos/parasitologia
Doenças dos Bovinos/prevenção & controle
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Nanopartículas/metabolismo
Nanopartículas/ultraestrutura
Extratos Vegetais/uso terapêutico
Folhas de Planta/química
Rhipicephalus/anatomia & histologia
Rhipicephalus/efeitos dos fármacos
Espectrofotometria Ultravioleta
Espectroscopia de Infravermelho com Transformada de Fourier
Infestações por Carrapato/tratamento farmacológico
Infestações por Carrapato/prevenção & controle
Difração de Raios X
Acetato de Zinco/farmacologia
Óxido de Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acaricides); 0 (Plant Extracts); FM5526K07A (Zinc Acetate); SOI2LOH54Z (Zinc Oxide)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160124
[St] Status:MEDLINE



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