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Pesquisa : B01.650.940.800.575.912.250.177.750 [Categoria DeCS]
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  1 / 92 MEDLINE  
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[PMID]:28667870
[Au] Autor:Nguyen PD; Sayagh C; Borie N; Lavaud C
[Ad] Endereço:Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312, BP 1039, 51687 Reims cedex 2, France.
[Ti] Título:Anti-radical flavonol glycosides from the aerial parts of Cleome chelidonii L.f.
[So] Source:Phytochemistry;142:30-37, 2017 Oct.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Eleven previously undescribed flavonoid glycosides, named cleomesides C-M, along with five known compounds, were isolated from the aerial parts of Cleome chelidonii L.f. (Cleomaceae). All flavonol glycosides were esterified derivatives of 3,7-O-diglycosides of quercetin or kaempferol. Their structures were elucidated by analysis of the 1D and 2D NMR spectra, HR-ESI-MS data, UV spectra, optical rotation and by comparison with literature data. The DPPH radical scavenging properties of the flavonoid glycosides were studied in order to appreciate the effect of the glycoside parts and of the ester groups on this activity compared with the quercetin and kaempferol aglycones. An acetate at position 3 of rhamnose linked to C-7 of flavonol, gave compounds with the strongest antiradical activity. An aromatic ester group at position 6 of terminal glucose of diglycoside chain linked to C-3 of flavonol did not seem to influence the antiradical activity.
[Mh] Termos MeSH primário: Cleome/química
Flavonóis/isolamento & purificação
Depuradores de Radicais Livres/isolamento & purificação
Glicosídeos/isolamento & purificação
Componentes Aéreos da Planta/química
[Mh] Termos MeSH secundário: Flavonóis/química
Depuradores de Radicais Livres/química
Glicosídeos/química
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonols); 0 (Free Radical Scavengers); 0 (Glycosides)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170702
[St] Status:MEDLINE


  2 / 92 MEDLINE  
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[PMID]:27731648
[Au] Autor:Moridi Farimani M; Nazarianpoor E; Rustaie A; Akhbari M
[Ad] Endereço:a Department of Phytochemistry, Medicinal Plants and Drugs Research Institute , Shahid Beheshti University , Tehran , Iran.
[Ti] Título:Phytochemical constituents and biological activities of Cleome iberica DC.
[So] Source:Nat Prod Res;31(11):1329-1332, 2017 Jun.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytochemical investigation of Cleome iberica DC. aerial parts led to the isolation and characterisation of six triterpenoid including glutinol (1), lupeol (2), ß-sitosterol (3), cabraleadiol monoacetate (4), cabraleadiol (6) and daucosterol (8), one flavonoid, calycopterin (5) along with a fatty acid, tricosanoic acid (7). While studied samples showed weak potency in antimicrobial assessment, the acetone extract and some isolated compounds exhibited considerable cytotoxicity against cervical cancer cell line (HeLa) in MTT assay, among them calycopterin with IC equals to 5.1 µM was the strongest. The extract showed moderate antioxidant capacity in DPPH assay method (IC = 100.2 µg/mL) and total phenolics and total flavonoids contents of the dried extract were also determined as 39.6 and 90.7 mg/g, respectively.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Cleome/química
Compostos Fitoquímicos/isolamento & purificação
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Morte Celular/efeitos dos fármacos
Flavonoides/isolamento & purificação
Células HeLa
Seres Humanos
Fenóis/isolamento & purificação
Compostos Fitoquímicos/farmacologia
Componentes Aéreos da Planta/química
Extratos Vegetais/química
Triterpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Flavonoids); 0 (Phenols); 0 (Phytochemicals); 0 (Plant Extracts); 0 (Triterpenes)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161013
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1239093


  3 / 92 MEDLINE  
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[PMID]:27925006
[Au] Autor:Cordeiro LD; Simoes-Gurgel C; Albarello N
[Ad] Endereço:Nucleo de Biotecnologia Vegetal (NBV), Instituto de Biologia Roberto Alcantara Gomes (IBRAG), Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, Brazil. livcordeiro@gmail.com.
[Ti] Título:CRYOPRESERVATION OF ADVENTITIOUS ROOTS OF Cleome rosea Vahl (CLEOMACEAE) USING A VITRIFICATION TECHNIQUE AND ASSESSMENT OF GENETIC STABILITY.
[So] Source:Cryo Letters;37(4):231-242, 2016 Jul/Aug.
[Is] ISSN:0143-2044
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cleome rosea, a Brazilian native species, has medicinal potential. Previously a cryopreservation protocol for in vitro roots using the vitrification solution PVS2 has been developed. However, the genetic stability of the cryopreserved material is yet to be assessed. OBJECTIVES: To evaluate the effects of loading and vitrification solutions (PVS2 and PVS3) on post-cryopreservation recovery of C. rosea roots, and to assess their genetic stability using Random Amplified Polymorphic DNA (RAPD) markers. MATERIALS AND METHODS: Root segments were pretreated with increasing concentrations of sucrose (0.2 to 0.4 M), followed by osmoprotection with loading solution and treatment with one of the vitrification solutions tested. RESULTS: The highest recoveries using PVS2 and PVS3 were obtained when root segments were exposed to these solutions for 15 min, reaching 77% and 100% respectively. The RAPD band profiles were monomorphic with most of the primers used. This molecular analysis revealed high genetic similarity (similarity coefficients among 0.98 and 1.00) between the cryopreserved roots and their mother plants. CONCLUSION: Roots from in vitro-propagated plants of C. rosea, were successfully cryopreserved using the vitrification technique. No major variations were observed on the genetic stability of cryopreserved roots, validating the use of this protocol as an efficient long-term conservation option for this species.
[Mh] Termos MeSH primário: Cleome/fisiologia
Criopreservação/métodos
Vitrificação
[Mh] Termos MeSH secundário: Cleome/genética
Raízes de Plantas/genética
Raízes de Plantas/fisiologia
Técnica de Amplificação ao Acaso de DNA Polimórfico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161208
[St] Status:MEDLINE


  4 / 92 MEDLINE  
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[PMID]:27618898
[Au] Autor:Ding HY; Wu PS; Wu MJ
[Ad] Endereço:Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan. hsiou221@yahoo.com.tw.
[Ti] Título:Cleome rutidosperma and Euphorbia thymifolia Suppress Inflammatory Response via Upregulation of Phase II Enzymes and Modulation of NF-κB and JNK Activation in LPS-Stimulated BV2 Microglia.
[So] Source:Int J Mol Sci;17(9), 2016 Aug 27.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Cleome rutidosperma DC. and Euphorbia thymifolia L. are herbal medicines used in traditional Indian and Chinese medicine to treat various illnesses. Reports document that they have antioxidant and anti-inflammatory activities; nonetheless, the molecular mechanisms involved in their anti-inflammatory actions have not yet been elucidated. The anti-neuroinflammatory activities and underlying mechanisms of ethanol extracts of Cleome rutidosperma (CR) and Euphorbia thymifolia (ET) were studied using lipopolysaccharide (LPS)-stimulated microglial cell line BV2. The morphology changes and production of pro-inflammatory mediators were assayed. Gene expression of inflammatory genes such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, interleukin (IL)-1ß, and CC chemokine ligand (CCL)-2, as well as phase II enzymes such as heme oxygenase (HO)-1, the modifier subunit of glutamate cysteine ligase (GCLM) and NAD(P)H quinone dehydrogenase 1 (NQO1), were further investigated using reverse transcription quantitative-PCR (RT-Q-PCR) and Western blotting. The effects of CR and ET on mitogen activated protein kinases (MAPKs) and nuclear factor (NF)-κB signaling pathways were examined using Western blotting and specific inhibitors. CR and ET suppressed BV2 activation, down-regulated iNOS and COX-2 expression and inhibited nitric oxide (NO) overproduction without affecting cell viability. They reduced LPS-mediated tumor necrosis factor (TNF) and IL-6 production, attenuated IL-1ß and CCL2 expression, but upregulated HO-1, GCLM and NQO1 expression. They also inhibited p65 NF-κB phosphorylation and modulated Jun-N terminal kinase (JNK) activation in BV2 cells. SP600125, the JNK inhibitor, significantly augmented the anti-IL-6 activity of ET. NF-κB inhibitor, Bay 11-7082, enhanced the anti-IL-6 effects of both CR and ET. Znpp, a competitive inhibitor of HO-1, attenuated the anti-NO effects of CR and ET. Our results show that CR and ET exhibit anti-neuroinflammatory activities by inhibiting pro-inflammatory mediator expression and production, upregulating HO-1, GCLM and NQO1, blocking NF-κB and modulating JNK signaling pathways. They may offer therapeutic potential for suppressing overactivated microglia and alleviating neurodegeneration.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Cleome/química
Euphorbia/química
Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo
Lipopolissacarídeos/farmacologia
Microglia/metabolismo
NF-kappa B/metabolismo
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Interleucina-6/metabolismo
Camundongos
Microglia/efeitos dos fármacos
Nitritos/metabolismo
Extratos Vegetais/química
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Interleukin-6); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (Nitrites); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha); EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE


  5 / 92 MEDLINE  
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[PMID]:27391957
[Au] Autor:Imanirampa L; Alele PE
[Ad] Endereço:Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, P.O. Box 1410, Mbarara, Uganda.
[Ti] Título:Antifungal activity of Cleome gynandra L. aerial parts for topical treatment of Tinea capitis: an in vitro evaluation.
[So] Source:BMC Complement Altern Med;16:194, 2016 Jul 08.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cleome gynandra L. (Capparaceae) is an edible weed used in Uganda topically for its presumed antifungal activity against Tinea capitis. The goal of this study was to determine if this plant possesses antifungal activity in vitro, since T. capitis is a pervasive infection among especially rural children. METHODS: Antifungal activity assay was performed by Broth dilution method, and testing done on clinical isolates of three common Tinea capitis-causing fungal strains. Evaluation of in vitro antifungal activity of the ethanol and water extracts of C. gynandra was done to determine the minimum inhibitory concentrations (MICs) and the minimum fungicidal concentrations (MFCs) of the extracts. RESULTS: The MIC of C. gynandra ethanol extract ranged from 0.0313 to 0.0625 mg/ml for Trichophyton rubrum, and from 0.25 to 0.5 mg/ml for both Microsporum canis and Trichophyton mentagrophytes. The MICs of C. gynandra aqueous extract ranged between 0.125 to 0.25 mg/ml for T. rubrum, and 0.25 to 0.5 mg/ml for both M. canis and T. mentagrophytes. T. rubrum was more sensitive than M. canis (p < 0.002) and more sensitive than T. mentagrophytes (p < 0.035) to the antifungal activity of C. gynandra. T. rubrum was 6.9 times (95 % CL: 1.15 - 41.6) more likely to have a better outcome (more sensitive) than T. mentagrophytes. Cleome gynandra aqueous extract had MFC of ≥0.0313 mg/ml for M. canis, ≥0.0156 mg/ml for T. mentagropyhtes, and ≥0.0625 mg/ml for T. rubrum. Cleome gynandra ethanol extract showed MFCs of ≥0.5 mg/ml for M. canis and T. mentagrophytes, and ≥0.125 mg/ml for T. rubrum. CONCLUSION: Both plant extracts demonstrated antifungal activity, shown by the MIC and MFC for the different extracts, which varied with the type of organism of the clinical fungal isolates. The ethanol extract exhibited comparable antifungal activity to the aqueous extract indicated by the MIC values seen. Conversely, after subculturing the fungal isolates, MFCs were lower for the aqueous than for the ethanol extract.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Cleome/química
Componentes Aéreos da Planta/química
Extratos Vegetais/farmacologia
Tinha do Couro Cabeludo/microbiologia
Trichophyton/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antifúngicos/química
Seres Humanos
Testes de Sensibilidade Microbiana
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1187-9


  6 / 92 MEDLINE  
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[PMID]:27032370
[Au] Autor:Tamboli AS; Patil SM; Gholave AR; Kadam SK; Kotibhaskar SV; Yadav SR; Govindwar SP
[Ad] Endereço:Department of Biochemistry, Shivaji University, Kolhapur 416004, India.
[Ti] Título:Phylogenetic analysis, genetic diversity and relationships between the recently segregated species of Corynandra and Cleoserrata from the genus Cleome using DNA barcoding and molecular markers.
[So] Source:C R Biol;339(3-4):123-32, 2016 Mar-Apr.
[Is] ISSN:1768-3238
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Cleome is the largest genus in the family Cleomaceae and it is known for its various medicinal properties. Recently, some species from the Cleome genus (Cleome viscosa, Cleome chelidonii, Cleome felina and Cleome speciosa) are split into genera Corynandra (Corynandra viscosa, Corynandra chelidonii, Corynandra felina), and Cleoserrata (Cleoserrata speciosa). The objective of this study was to obtain DNA barcodes for these species for their accurate identification and determining phylogenetic relationships. Out of 10 screened barcoding regions, rbcL, matK and ITS1 regions showed higher PCR efficiency and sequencing success. This study added matK, rbcL and ITS1 barcodes for the identification of Corynandra chelidonii, Corynandra felina, Cleome simplicifolia and Cleome aspera species in existing barcode data. Corynandra chelidonii and Corynandra felina species belong to the Corynandra genus, but they are not grouped with the Corynandra viscosa species, however clustered with the Cleome species. Molecular marker analysis showed 100% polymorphism among the studied plant samples. Diversity indices for molecular markers were ranged from He=0.1115-0.1714 and I=0.2268-0.2700, which indicates a significant amount of genetic diversity among studied species. Discrimination of the Cleome and Corynandra species from Cleoserrata speciosa was obtained by two RAPD primers (OPA-4 and RAPD-17) and two ISSR primers (ISSR-1 and ISSR-2). RAPD and ISSR markers are useful for the genetic characterization of these studied species. The present investigation will be helpful to understand the relationships of Cleome lineages with Corynandra and Cleoserrata species.
[Mh] Termos MeSH primário: Cleome/genética
Código de Barras de DNA Taxonômico
DNA de Plantas
Variação Genética
[Mh] Termos MeSH secundário: Cleome/classificação
Marcadores Genéticos
Filogenia
Reação em Cadeia da Polimerase
Polimorfismo Genético
Técnica de Amplificação ao Acaso de DNA Polimórfico
Análise de Sequência de DNA
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Plant); 0 (Genetic Markers)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE


  7 / 92 MEDLINE  
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[PMID]:26909753
[Au] Autor:Sarhan WA; Azzazy HM; El-Sherbiny IM
[Ad] Endereço:Department of Chemistry, School of Sciences & Engineering, The American University in Cairo , New Cairo 11835, Egypt.
[Ti] Título:Honey/Chitosan Nanofiber Wound Dressing Enriched with Allium sativum and Cleome droserifolia: Enhanced Antimicrobial and Wound Healing Activity.
[So] Source:ACS Appl Mater Interfaces;8(10):6379-90, 2016 Mar.
[Is] ISSN:1944-8252
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two natural extracts were loaded within fabricated honey, poly(vinyl alcohol), chitosan nanofibers (HPCS) to develop biocompatible antimicrobial nanofibrous wound dressing. The dried aqueous extract of Cleome droserifolia (CE) and Allium sativum aqueous extract (AE) and their combination were loaded within the HPCS nanofibers in the HPCS-CE, HPCS-AE, and HPCS-AE/CE nanofiber mats, respectively. It was observed that the addition of AE resulted in the least fiber diameter (145 nm), whereas the addition of the AE and CE combination resulted in the least swelling ability and the highest weight loss. In vitro antibacterial testing against Staphylococcus aureus, Escherichia coli, Methicillin-resistant S. aureus (MRSA), and multidrug-resistant Pseudomonas aeruginosa was performed in comparison with the commercial dressing AquacelAg and revealed that the HPCS-AE and HPCS-AE/CE nanofiber mats allowed complete inhibition of S. aureus and the HPCS-AE/CE exhibited mild antibacterial activity against MRSA. A preliminary in vivo study revealed that the developed nanofiber mats enhanced the wound healing process as compared to the untreated control as proved by the enhanced wound closure rates in mice and by the histological examination of the wounds. Moreover, comparison with the commercial dressing Aquacel Ag, the HPCS, and HPCS-AE/CE demonstrated similar effects on the wound healing process, whereas the HPCS/AE allowed an enhanced wound closure rate. Cell culture studies proved the biocompatibility of the developed nanofiber mats in comparison with the commercial Aquacel Ag, which exhibited noticeable cytotoxicity. The developed natural nanofiber mats hold potential as promising biocompatible antibacterial wound dressing.
[Mh] Termos MeSH primário: Anti-Infecciosos
Bactérias/crescimento & desenvolvimento
Bandagens
Quitosana/química
Cleome/química
Alho/química
Mel
Nanofibras/química
Cicatrização/efeitos dos fármacos
Infecção dos Ferimentos/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Masculino
Camundongos
Infecção dos Ferimentos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.1021/acsami.6b00739


  8 / 92 MEDLINE  
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[PMID]:26818731
[Au] Autor:Aubry S; Aresheva O; Reyna-Llorens I; Smith-Unna RD; Hibberd JM; Genty B
[Ad] Endereço:Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom (S.A., I.R.-L., R.D.S.-U., J.M.H.); andCommissariat à l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7265, and Université Aix-Marseille, B
[Ti] Título:A Specific Transcriptome Signature for Guard Cells from the C4 Plant Gynandropsis gynandra.
[So] Source:Plant Physiol;170(3):1345-57, 2016 Mar.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:C4 photosynthesis represents an excellent example of convergent evolution that results in the optimization of both carbon and water usage by plants. In C4 plants, a carbon-concentrating mechanism divided between bundle sheath and mesophyll cells increases photosynthetic efficiency. Compared with C3 leaves, the carbon-concentrating mechanism of C4 plants allows photosynthetic operation at lower stomatal conductance, and as a consequence, transpiration is reduced. Here, we characterize transcriptomes from guard cells in C3 Tareneya hassleriana and C4 Gynandropsis gynandra belonging to the Cleomaceae. While approximately 60% of Gene Ontology terms previously associated with guard cells from the C3 model Arabidopsis (Arabidopsis thaliana) are conserved, there is much less overlap between patterns of individual gene expression. Most ion and CO2 signaling modules appear unchanged at the transcript level in guard cells from C3 and C4 species, but major variations in transcripts associated with carbon-related pathways known to influence stomatal behavior were detected. Genes associated with C4 photosynthesis were more highly expressed in guard cells of C4 compared with C3 leaves. Furthermore, we detected two major patterns of cell-specific C4 gene expression within the C4 leaf. In the first, genes previously associated with preferential expression in the bundle sheath showed continually decreasing expression from bundle sheath to mesophyll to guard cells. In the second, expression was maximal in the mesophyll compared with both guard cells and bundle sheath. These data imply that at least two gene regulatory networks act to coordinate gene expression across the bundle sheath, mesophyll, and guard cells in the C4 leaf.
[Mh] Termos MeSH primário: Cleome/citologia
Cleome/genética
[Mh] Termos MeSH secundário: Arabidopsis/citologia
Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Magnoliopsida/citologia
Magnoliopsida/genética
Células do Mesofilo/metabolismo
Fotossíntese/genética
Folhas de Planta/citologia
Folhas de Planta/metabolismo
Estômatos de Plantas/citologia
Estômatos de Plantas/metabolismo
Transdução de Sinais
Especificidade da Espécie
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160129
[St] Status:MEDLINE
[do] DOI:10.1104/pp.15.01203


  9 / 92 MEDLINE  
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[PMID]:26772995
[Au] Autor:Williams BP; Burgess SJ; Reyna-Llorens I; Knerova J; Aubry S; Stanley S; Hibberd JM
[Ad] Endereço:Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom.
[Ti] Título:An Untranslated cis-Element Regulates the Accumulation of Multiple C4 Enzymes in Gynandropsis gynandra Mesophyll Cells.
[So] Source:Plant Cell;28(2):454-65, 2016 Feb.
[Is] ISSN:1532-298X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:C4 photosynthesis is a complex phenotype that allows more efficient carbon capture than the ancestral C3 pathway. In leaves of C4 species, hundreds of transcripts increase in abundance compared with C3 relatives and become restricted to mesophyll (M) or bundle sheath (BS) cells. However, no mechanism has been reported that regulates the compartmentation of multiple enzymes in M or BS cells. We examined mechanisms regulating CARBONIC ANHYDRASE4 (CA4) in C4 Gynandropsis gynandra. Increased abundance is directed by both the promoter region and introns of the G. gynandra gene. A nine-nucleotide motif located in the 5' untranslated region (UTR) is required for preferential accumulation of GUS in M cells. This element is present and functional in three additional 5' UTRs and six 3' UTRs where it determines accumulation of two isoforms of CA and pyruvate,orthophosphate dikinase in M cells. Although the GgCA4 5' UTR is sufficient to direct GUS accumulation in M cells, transcripts encoding GUS are abundant in both M and BS. Mutating the GgCA4 5' UTR abolishes enrichment of protein in M cells without affecting transcript abundance. The work identifies a mechanism that directs cell-preferential accumulation of multiple enzymes required for C4 photosynthesis.
[Mh] Termos MeSH primário: Cleome/genética
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Anidrases Carbônicas/genética
Anidrases Carbônicas/metabolismo
Cleome/citologia
Cleome/enzimologia
Genes Reporter
Íntrons/genética
Células do Mesofilo/enzimologia
Fotossíntese/genética
Folhas de Planta/citologia
Folhas de Planta/enzimologia
Folhas de Planta/genética
Proteínas de Plantas/genética
Regiões Promotoras Genéticas/genética
Alinhamento de Sequência
Regiões não Traduzidas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Untranslated Regions); EC 4.2.1.1 (Carbonic Anhydrases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160117
[St] Status:MEDLINE
[do] DOI:10.1105/tpc.15.00570


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[PMID]:26490448
[Au] Autor:Jiménez-Aguilar DM; Grusak MA
[Ad] Endereço:USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 1100 Bates Avenue, Houston, TX, 77030-2600, USA.
[Ti] Título:Evaluation of Minerals, Phytochemical Compounds and Antioxidant Activity of Mexican, Central American, and African Green Leafy Vegetables.
[So] Source:Plant Foods Hum Nutr;70(4):357-64, 2015 Dec.
[Is] ISSN:1573-9104
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The green leafy vegetables Cnidoscolus aconitifolius and Crotalaria longirostrata are native to Mexico and Central America, while Solanum scabrum and Gynandropsis gynandra are native to Africa. They are consumed in both rural and urban areas in those places as a main food, food ingredient or traditional medicine. Currently, there is limited information about their nutritional and phytochemical composition. Therefore, mineral, vitamin C, phenolic and flavonoid concentration, and antioxidant activity were evaluated in multiple accessions of these leafy vegetables, and their mineral and vitamin C contribution per serving was calculated. The concentrations of Ca, K, Mg and P in these leafy vegetables were 0.82-2.32, 1.61-7.29, 0.61-1.48 and 0.27-1.44 mg/g fresh weight (FW), respectively. The flavonoid concentration in S. scabrum accessions was up to 1413 µg catechin equivalents/g FW, while the highest antioxidant activities were obtained in C. longirostrata accessions (52-60 µmol Trolox equivalents/g FW). According to guidelines established by the US Food and Drug Administration, a serving size (30 g FW) of C. longirostrata would be considered an excellent source of Mo (20 % or more of the daily value), and a serving of any of these green leafy vegetables would be an excellent source of vitamin C. Considering the importance of the minerals, phytochemicals and antioxidants in human health and their presence in these indigenous green leafy vegetables, efforts to promote their consumption should be implemented.
[Mh] Termos MeSH primário: Antioxidantes/análise
Minerais/análise
Compostos Fitoquímicos/análise
Verduras/química
[Mh] Termos MeSH secundário: África
Ácido Ascórbico/análise
América Central
Cleome/química
Crotalaria/química
Euphorbiaceae/química
Flavonoides/análise
Alimentos
Seres Humanos
Medicina Tradicional
México
Fenóis/análise
Folhas de Planta/química
Solanum/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antioxidants); 0 (Flavonoids); 0 (Minerals); 0 (Phenols); 0 (Phytochemicals); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151023
[St] Status:MEDLINE
[do] DOI:10.1007/s11130-015-0512-7



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