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Pesquisa : B01.650.940.800.575.912.250.192 [Categoria DeCS]
Referências encontradas : 22 [refinar]
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  1 / 22 MEDLINE  
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[PMID]:26657577
[Au] Autor:Nghonjuyi NW; Tiambo CK; Taïwe GS; Toukala JP; Lisita F; Juliano RS; Kimbi HK
[Ad] Endereço:Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, P.O. Box 63 Buea, South West Region, Cameroon.
[Ti] Título:Acute and sub-chronic toxicity studies of three plants used in Cameroonian ethnoveterinary medicine: Aloe vera (L.) Burm. f. (Xanthorrhoeaceae) leaves, Carica papaya L. (Caricaceae) seeds or leaves, and Mimosa pudica L. (Fabaceae) leaves in Kabir chicks.
[So] Source:J Ethnopharmacol;178:40-9, 2016 Feb 03.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Aloe vera (L.) Burm. f. (Xanthorrhoeaceae), Carica papaya L. (Caricaceae) and Mimosa pudica L. (Fabaceae) are widely used in the Cameroonian ethnoveterinary medicine as a panacea, and specifically for gastrointestinal disorders as well as an anthelmintic and antibacterial. AIM OF THE STUDY: The present study evaluated the potential toxicity of the hydroalcoholic extracts of Aloe vera leaves, Carica papaya leaves or seeds, and Mimosa pudica leaves after acute and sub-chronic administration in chicks. MATERIALS AND METHODS: For the acute toxicity test a single administration of each of the four hydroalcoholic extracts was given orally at doses ranging from 40 to 5120 mg/kg (n=5/group/sex). In the sub-chronic study, these extracts were given orally as a single administration to chicks at doses of 80, 160, 320 and 640 mg/kg/day for 42 days. The anti-angiogenic properties of these extracts (5-320 µg/mg) were investigated in the chick chorioallantoic membrane in vivo. RESULTS: In the acute toxicity test, none of the four studied hydroalcoholic extracts induced mortality or significant behavioural changes. The sub-acute treatment with the four plant extracts did not alter either the body weight gain or the food and water consumption. However, the results indicated that Aloe vera leaf extract acute treatment by oral route at doses up to 2560 mg/kg did not produce death in 50% (5/10) of chicks during 24h or 14 days of observation, but 20% (2/10) chicks died. The haematological and biochemical analyses did not show significant differences in any of the parameters examined in female or male groups, with the exception of a transient rise in white blood cell counts at high doses (640 mg/kg). Additionally, these extracts did not have the potential for anti-angiogenic effects through the inhibition of neo-angiogenesis in the chick chorioallantoic membrane in vivo. CONCLUSION: The results showed that the therapeutic use of the hydroalcoholic extracts of Aloe vera leaves, Carica papaya leaves or seeds and Mimosa pudica leaves had very low toxicity in oral acute high dose administration and no toxicity in oral sub-chronic low dose administration and indicate that the plants could be considered safe for oral medication in chicks.
[Mh] Termos MeSH primário: Aloe/toxicidade
Carica/toxicidade
Caricaceae/toxicidade
Mimosa/toxicidade
Folhas de Planta/toxicidade
Sementes/toxicidade
[Mh] Termos MeSH secundário: Animais
Galinhas
Fabaceae/toxicidade
Feminino
Masculino
Extratos Vegetais/toxicidade
Testes de Toxicidade Aguda
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Extracts)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE


  2 / 22 MEDLINE  
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[PMID]:26227064
[Au] Autor:Morales-Quintana L; Moya-León MA; Herrera R
[Ad] Endereço:Laboratorio de Fisiología Vegetal y Genética Molecular, Instituto de Ciencias Biológicas, Universidad de Talca, Casilla 747, Talca, Chile.
[Ti] Título:Computational study enlightens the structural role of the alcohol acyltransferase DFGWG motif.
[So] Source:J Mol Model;21(8):216, 2015 Aug.
[Is] ISSN:0948-5023
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Alcohol acyltransferases (AAT) catalyze the esterification reaction of alcohols and acyl-CoA into esters in fruits and flowers. Despite the high divergence between AAT enzymes, two important and conserved motifs are shared: the catalytic HxxxD motif, and the DFGWG motif. The latter is proposed to play a structural role; however, its function remains unclear. The DFGWG motif is located in loop 21 and stabilized by a hydrogen bond between residues Y52 and D381. Also, this motif is distant from the HxxxD motif, and most probably without a direct role in the substrate interaction. To evaluate the role of the DFGWG motif, in silico analysis was performed in the VpAAT1 protein. Three mutants (Y52F, D381A and D381E) were evaluated. Major changes (size and shape) in the solvent channels were found, although no differences were revealed in the entire 3D structure. Molecular dynamics simulations and docking studies described unfavorable energies for interaction of the mutant proteins with different substrates, as well as unfavored ligand orientations in the solvent channel. Additionally, we examined the contribution of different energetic parameters to the total free energy of protein-ligand complexes by the MM-GBSA method. The complexes differed mainly in their van der Waals contributions and have unfavorable electrostatic interactions. VpAAT1, Y52F and D381A mutants showed a dramatic reduction in the binding capacity to several substrates, which is related to differences in electrostatic potential on the protein surfaces, suggesting that D381 from the DFGWG motif and residue Y52 play a crucial role in maintenance of the adequate solvent channel structure required for catalysis. Graphical abstract Molecular docking, molecular dynamics (MD) simulations and MM-GBSA free energy calculations were employed to obtain quantitative estimates for the binding free energies of wild type Vasconcellea pubescens alcohol acyltransferase (VpAAT1-WT) and the protein mutants. Left VpAAT1 model structure in cartoon representation showing the solvent channel in the middle of the structure. Center, right Changes in shape and structure in the solvent channel of Y52F and D381A mutant proteins, respectively, compared to WT. The results obtained reveal that the interaction between D381 and Y52 residues is important for the maintenance of solvent channel structure.
[Mh] Termos MeSH primário: Caricaceae/enzimologia
Simulação de Dinâmica Molecular
Proteínas/química
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Simulação por Computador
Mutação
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Estrutura Terciária de Proteína
Proteínas/genética
Proteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Proteins); EC 2.3.1.84 (alcohol O-acetyltransferase)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150801
[St] Status:MEDLINE
[do] DOI:10.1007/s00894-015-2762-6


  3 / 22 MEDLINE  
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[PMID]:25480779
[Au] Autor:Iovene M; Yu Q; Ming R; Jiang J
[Ad] Endereço:Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706 Consiglio Nazionale delle Ricerche-Institute of Biosciences and BioResouces, Bari 70126, Italy.
[Ti] Título:Evidence for emergence of sex-determining gene(s) in a centromeric region in Vasconcellea parviflora.
[So] Source:Genetics;199(2):413-21, 2015 Feb.
[Is] ISSN:1943-2631
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sex chromosomes have been studied in many plant and animal species. However, few species are suitable as models to study the evolutionary histories of sex chromosomes. We previously demonstrated that papaya (Carica papaya) (2n = 2x = 18), a fruit tree in the family Caricaceae, contains recently emerged but cytologically heteromorphic X/Y chromosomes. We have been intrigued by the possible presence and evolution of sex chromosomes in other dioecious Caricaceae species. We selected a set of 22 bacterial artificial chromosome (BAC) clones that are distributed along the papaya X/Y chromosomes. These BACs were mapped to the meiotic pachytene chromosomes of Vasconcellea parviflora (2n = 2x = 18), a species that diverged from papaya ∼27 million years ago. We demonstrate that V. parviflora contains a pair of heteromorphic X/Y chromosomes that are homologous to the papaya X/Y chromosomes. The comparative mapping results revealed that the male-specific regions of the Y chromosomes (MSYs) probably initiated near the centromere of the Y chromosomes in both species. The two MSYs, however, shared only a small chromosomal domain near the centromere in otherwise rearranged chromosomes. The V. parviflora MSY expanded toward the short arm of the chromosome, whereas the papaya MSY expanded in the opposite direction. Most BACs mapped to papaya MSY were not located in V. parviflora MSY, revealing different DNA compositions in the two MSYs. These results suggest that mutation of gene(s) in the centromeric region may have triggered sex chromosome evolution in these plant species.
[Mh] Termos MeSH primário: Caricaceae/genética
Centrômero
Genes de Plantas
Diferenciação Sexual/genética
[Mh] Termos MeSH secundário: Caricaceae/classificação
Cromossomos de Plantas
Flores
Heterocromatina
Hibridização in Situ Fluorescente
Fenótipo
Filogenia
Mapeamento Físico do Cromossomo
Cromossomos Sexuais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Heterochromatin)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:160202
[Lr] Data última revisão:
160202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141207
[St] Status:MEDLINE
[do] DOI:10.1534/genetics.114.173021


  4 / 22 MEDLINE  
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[PMID]:24032548
[Au] Autor:Morales-Quintana L; Nuñez-Tobar MX; Moya-León MA; Herrera R
[Ad] Endereço:Laboratorio de Fisiología Vegetal y Genética Molecular, Instituto de Biología Vegetal y Biotecnología, Universidad de Talca , Casilla 747, Talca, Chile 3465548.
[Ti] Título:Molecular dynamics simulation and site-directed mutagenesis of alcohol acyltransferase: a proposed mechanism of catalysis.
[So] Source:J Chem Inf Model;53(10):2689-700, 2013 Oct 28.
[Is] ISSN:1549-960X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aroma in Vasconcellea pubescens fruit is determined by esters, which are the products of catalysis by alcohol acyltransferase (VpAAT1). VpAAT1 protein structure displayed the conserved HxxxD motif facing the solvent channel in the center of the structure. To gain insight into the role of these catalytic residues, kinetic and site-directed mutagenesis studies were carried out in VpAAT1 protein. Based on dead-end inhibition studies, the kinetic could be described in terms of a ternary complex mechanism with the H166 residue as the catalytic base. Kinetic results showed the lowest Km value for hexanoyl-CoA. Additionally, the most favorable predicted substrate orientation was observed for hexanoyl-CoA, showing a coincidence between kinetic studies and molecular docking analysis. Substitutions H166A, D170A, D170N, and D170E were evaluated in silico. The solvent channel in all mutant structures was lost, showing large differences with the native structure. Molecular docking and molecular dynamics simulations were able to describe unfavored energies for the interaction of the mutant proteins with different alcohols and acyl-CoAs. Additionally, in vitro site-directed mutagenesis of H166 and D170 in VpAAT1 induced a loss of activity, confirming the functional role of both residues for the activity, H166 being directly involved in catalysis.
[Mh] Termos MeSH primário: Acil Coenzima A/química
Aciltransferases/química
Caricaceae/enzimologia
Frutas/enzimologia
Simulação de Dinâmica Molecular
Proteínas de Plantas/química
[Mh] Termos MeSH secundário: Aciltransferases/genética
Motivos de Aminoácidos
Biocatálise
Caricaceae/química
Domínio Catalítico
Ensaios Enzimáticos
Escherichia coli/genética
Escherichia coli/metabolismo
Frutas/química
Cinética
Simulação de Acoplamento Molecular
Mutagênese Sítio-Dirigida
Proteínas de Plantas/genética
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acyl Coenzyme A); 0 (Plant Proteins); 0 (Recombinant Proteins); 5060-32-2 (hexanoyl-coenzyme A); EC 2.3.- (Acyltransferases)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:131028
[Lr] Data última revisão:
131028
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130917
[St] Status:MEDLINE
[do] DOI:10.1021/ci400409s


  5 / 22 MEDLINE  
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[PMID]:22790602
[Au] Autor:Torres MJ; Trejo SA; Obregón WD; Avilés FX; López LM; Natalucci CL
[Ad] Endereço:Laboratorio de Investigación de Proteínas Vegetales, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, C.C. 711, B1900AVW, La Plata, Argentina.
[Ti] Título:Characterization of the proteolytic system present in Vasconcellea quercifolia latex.
[So] Source:Planta;236(5):1471-84, 2012 Nov.
[Is] ISSN:1432-2048
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Vasconcellea quercifolia (Caricaceae) latex contains several cysteine endopeptidases with high proteolytic activity. Cysteine endopeptidases are the main active compounds used by the plant as a defense mechanism. A proteolytic preparation from V. quercifolia ("oak leaved papaya") latex was purified by cation exchange chromatography. From SDS-PAGE and blotting of the selected fractions, the N-terminal amino acid sequences of polypeptides were determined by Edman's degradation. The analysis by peptide mass fingerprinting (PMF) of the enzymes allowed their characterization and confirmed the presence of seven different cysteine proteinases in the latex of V. quercifolia. Moreover, the comparison between the tryptic maps with those deposited in databases using the MASCOT tool showed that none of the isolated proteases matched with another plant protease. Notably, a propeptidase was detected in the plant latex, which is being the first report in this sense. Furthermore, the cDNA of one of the cysteine proteases that is expressed in the latex of V. quercifolia was cloned and sequenced. The consensus sequence was aligned using the ClustalX web server, which allowed detecting a high degree of identity with cysteine proteases of the Caricaceae family and establishing the evolutionary relationship between them. We also observed a high conservation degree for those amino acid residues which are essential for the catalytic activity and tridimensional structure of the plant proteases belonging to the subfamily C1A. The PMF analysis strongly suggests that the sequence obtained corresponds to the VQ-III peptidase.
[Mh] Termos MeSH primário: Caricaceae/química
Látex/metabolismo
Peptídeo Hidrolases/química
Peptídeo Hidrolases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Caricaceae/metabolismo
Clonagem Molecular
Sequência Conservada
Cisteína Proteases/química
Cisteína Proteases/genética
Cisteína Proteases/metabolismo
Eletroforese em Gel de Poliacrilamida
Modelos Moleculares
Dados de Sequência Molecular
Peptídeo Hidrolases/isolamento & purificação
Mapeamento de Peptídeos
Filogenia
Proteínas de Plantas/análise
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Estrutura Terciária de Proteína
Proteólise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Latex); 0 (Plant Proteins); EC 3.4.- (Cysteine Proteases); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120714
[St] Status:MEDLINE
[do] DOI:10.1007/s00425-012-1701-3


  6 / 22 MEDLINE  
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[PMID]:22543019
[Au] Autor:Ramos-Martínez EM; Herrera-Ramírez AC; Badillo-Corona JA; Garibay-Orijel C; González-Rábade N; Oliver-Salvador Mdel C
[Ad] Endereço:Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, México DF, Mexico.
[Ti] Título:Isolation of cDNA from Jacaratia mexicana encoding a mexicain-like cysteine protease gene.
[So] Source:Gene;502(1):60-8, 2012 Jul 01.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cysteine proteases (CPs) from the C1 family, which are similar to papain, can be found in animals and plants, as well as some viruses and prokaryotes. These enzymes have diverse physiological functions and are thus very attractive for science and industry. Jacaratia mexicana, a member of the Caricaceae plant family, contains several CPs, the principal being mexicain, found to favorably compete against papain for many industrial applications due to its high stability and specific activity. In this study, leaves of J. mexicana were used to isolate a CP-coding gene, similar to those that code for mexicain and chymomexicain. By using rapid amplification of cDNA ends (RACE) as well as oligonucleotide design from papain-like conserved amino acids (aa), a sequence of 1404 bp consisting of a 5' terminal untranslated region (UTR) of 153 bp, a 3' terminal UTR of 131 bp, with a polyadenylation (poly(A)) signal sequence and a poly(A) tail, and an open reading frame (ORF) of 1046 bp, was obtained by overlapping three partial sequences. Two full-length cDNA sequences that encode for mexicain-like proteases were cloned from mRNA (JmCP4 and JmCP5). JmCP4 is predicted to have an ORF of 1044 bp, which codifies for polypeptides that have a 26 aa signal peptide region, a 108 aa propeptide region and a mature enzyme of 214 aa. A 969 bp fragment (JmCP5) encodes for a partial sequence of a CP gene, without the signal peptide region but with a full-length propeptide region. The sequence analysis showed that this protease presented a high similarity to other plant CPs from J. mexicana, Vasconcellea cundinamarcensis, Vasconcellea stipulata, and Carica papaya, among others, mainly at the conserved catalytic site. Obtaining the sequence of this CP gene from J. mexicana provides an alternative for production in a standard system and could be an initial step towards the commercialization of this enzyme.
[Mh] Termos MeSH primário: Caricaceae/genética
Cisteína Proteases/genética
Proteínas de Plantas/genética
Precursores de Proteínas/genética
RNA de Plantas/isolamento & purificação
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Sequência de Aminoácidos
Sequência de Bases
Caricaceae/enzimologia
Domínio Catalítico
Cisteína Proteases/biossíntese
Cisteína Proteases/química
DNA Complementar/biossíntese
Estabilidade Enzimática
Isoenzimas/biossíntese
Isoenzimas/química
Isoenzimas/genética
Modelos Moleculares
Dados de Sequência Molecular
Folhas de Planta/genética
Proteínas de Plantas/biossíntese
Proteínas de Plantas/química
Precursores de Proteínas/química
RNA Mensageiro/genética
RNA Mensageiro/isolamento & purificação
RNA de Plantas/genética
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Homologia Estrutural de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Isoenzymes); 0 (Plant Proteins); 0 (Protein Precursors); 0 (RNA, Messenger); 0 (RNA, Plant); 0 (Recombinant Proteins); EC 3.4.- (Cysteine Proteases)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:120516
[Lr] Data última revisão:
120516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120501
[St] Status:MEDLINE
[do] DOI:10.1016/j.gene.2012.04.018


  7 / 22 MEDLINE  
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[PMID]:22458206
[Au] Autor:Arias DM; Albarrán-Lara AL; González-Rodríguez A; Peñaloza-Ramírez J; Dorado O; Leyva E
[Ad] Endereço:Centro de Educación Ambiental e Investigación Sierra de Huautla (CEAMISH), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, 62210, Morelos, México. dulce@uaem.mx
[Ti] Título:Genetic diversity and structure of wild populations of the tropical dry forest tree Jacaratia mexicana (Brassicales: Caricaceae) at a local scale in Mexico.
[So] Source:Rev Biol Trop;60(1):1-10, 2012 Mar.
[Is] ISSN:0034-7744
[Cp] País de publicação:Costa Rica
[La] Idioma:eng
[Ab] Resumo:The tropical dry forest is a greatly endangered ecosystem, from which Jacaratia mexicana is a native tree. With the aim to assess the levels of genetic variation and population structure, four wild populations of J. mexicana were studied in the Sierra de Huautla Biosphere Reserve, Morelos, Mexico. For this, DNA was extracted from 159 individuals and were amplified with six random primers using the Random Amplified Polymorphic DNA (RAPD). A total of 54 bands were obtained, of which 50 (92.6%) were polymorphic. The total genetic diversity found within the four populations was 0.451 when estimated by Shannon's index. An AMOVA analysis showed that 84% of the total genetic variation was found within populations and 16% was among populations. The UPGMA dendrogram showed that all individuals from one of the populations (Huaxtla) formed one distinct genetic group, while the rest of the individuals did not cluster according to population. A Mantel test did not show an association between genetic and geographical distances among populations (r=0.893, p=0.20). A Bayesian cluster analysis performed with STRUCTURE, showed that the most probable number of genetic groups in the data was four (K=4), and confirmed the distinctness of Huaxtla population. Our results showed that important genetic differentiation among populations can occur even at this small geographic scale and this has to be considered in conservation actions for this genetic resource.
[Mh] Termos MeSH primário: Caricaceae/genética
Variação Genética
Árvores/genética
[Mh] Termos MeSH secundário: Teorema de Bayes
DNA de Plantas/análise
México
Técnica de Amplificação ao Acaso de DNA Polimórfico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:120330
[Lr] Data última revisão:
120330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120331
[St] Status:MEDLINE


  8 / 22 MEDLINE  
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[PMID]:22382056
[Au] Autor:Shi JS; Wang ZJ; Chen JH
[Ad] Endereço:Nanjing Forestry University, Nanjing, China. jshi@njfu.edu.cn
[Ti] Título:[Progress on whole genome sequencing in woody plants].
[So] Source:Yi Chuan;34(2):145-56, 2012 Feb.
[Is] ISSN:0253-9772
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:In recent years, the number of sequencing data of plant whole genome have been increasing rapidly and the whole genome sequencing has been also performed widely in woody plants. However, there are a set of obstacles in investigating the whole genome sequencing in woody plants, which include larger genome, complex genome structure, limitations of assembly, annotation, functional analysis, and restriction of the funds for scientific research. Therefore, to promote the efficiency of the whole genome sequencing in woody plants, the development and defect of this field should be analyzed. The three-generation sequencing technologies (i.e., Sanger sequencing, synthesis sequencing, and single molecule sequencing) were compared in our studies. The progress mainly focused on the whole genome sequencing in four woody plants (Populus, Grapevine, Papaya, and Apple), and the application of sequencing results also was analyzed. The future of whole genome sequencing research in woody plants, consisting of material selection, establishment of genetic map and physical map, selection of sequencing technology, bioinformatic analysis, and application of sequencing results, was discussed.
[Mh] Termos MeSH primário: Genoma de Planta
Análise de Sequência de DNA/métodos
Árvores/genética
[Mh] Termos MeSH secundário: Caricaceae/genética
Rosácea/genética
Vitis/genética
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1204
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120303
[St] Status:MEDLINE


  9 / 22 MEDLINE  
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[PMID]:20873836
[Au] Autor:Torres MJ; Trejo SA; Martin MI; Natalucci CL; Avilés FX; López LM
[Ad] Endereço:Laboratorio de Investigación de Proteínas Vegetales (LIPROVE), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, C.C. 711, B1900AVW, La Plata, Argentina.
[Ti] Título:Purification and characterization of a cysteine endopeptidase from Vasconcellea quercifolia A. St.-Hil. latex displaying high substrate specificity.
[So] Source:J Agric Food Chem;58(20):11027-35, 2010 Oct 27.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new proteolytic preparation from Vasconcellea quercifolia ("oak leaved papaya") latex containing several cysteine endopeptidases with high proteolytic activity has been obtained. The specific activity of the new enzymatic preparation (VQ) was higher than that of Carica papaya latex. VQ was able to coagulate milk and to hydrolyze caseins and then could be used to produce cheeses and/or casein hydrolysates. Ion exchange chromatography of VQ allowed the isolation of a new protease, named quercifoliain I, homogeneous when analyzed by SDS-PAGE, IEF and MALDI-TOF-MS. Molecular mass was 24195 Da, and its isoelectric point was >9.3. The N-terminal sequence was determined (YPESVDWRQ). Insulin B-chain cleavage showed higher specificity than that of papain and was restricted to glycyl and alanyl residues at P1' position. The tryptic peptide mass fingerprint of quercifoliain I analyzed with the MASCOT search tool did not find a match with papain or any other plant cysteine proteases.
[Mh] Termos MeSH primário: Caricaceae/enzimologia
Cisteína Endopeptidases/química
Cisteína Endopeptidases/isolamento & purificação
Látex/química
Proteínas de Plantas/química
Proteínas de Plantas/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Biocatálise
Caricaceae/química
Caricaceae/genética
Cisteína Endopeptidases/genética
Cisteína Endopeptidases/metabolismo
Estabilidade Enzimática
Insulina/química
Insulina/metabolismo
Ponto Isoelétrico
Dados de Sequência Molecular
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insulin); 0 (Latex); 0 (Plant Proteins); EC 3.4.22.- (Cysteine Endopeptidases)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140204
[Lr] Data última revisão:
140204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100930
[St] Status:MEDLINE
[do] DOI:10.1021/jf904295x


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[PMID]:20579309
[Au] Autor:Wu X; Wang J; Na JK; Yu Q; Moore RC; Zee F; Huber SC; Ming R
[Ad] Endereço:Program in Physiological and Molecular Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 16801, USA.
[Ti] Título:The origin of the non-recombining region of sex chromosomes in Carica and Vasconcellea.
[So] Source:Plant J;63(5):801-10, 2010 Sep.
[Is] ISSN:1365-313X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Carica and Vasconcellea are two closely related sister genera in the family Caricaceae, and were once classified as two sections under Carica. Sex chromosomes have been found in papaya and originated approximately 2-3 million years ago. The objectives of this study were to determine whether sex chromosomes have evolved in Vasconcellea. Six X/Y gene pairs were cloned, sequenced and analyzed from three dioecious, one trioecious and one monoecious species of Vasconcellea. The isolation of distinctive X and Y alleles in dioecious and trioecious species of Vasconcellea demonstrated that sex chromosomes have evolved in this genus. Phylogenetic analyses indicated a monophyletic relationship between the X/Y alleles of Carica and those of Vasconcellea. Distinctive clusters of X/Y alleles were documented in V. parviflora and V. pulchra for all available gene sequences, and in V. goudatinana and V. cardinamarcensis for some X/Y alleles. The X and Y alleles within each species shared most single nucleotide polymorphism haplotypes that differed from other species. Limited evidence of gene conversion was documented among the X/Y alleles of some species, but was not sufficient to cause the evolutionary patterns reported herein. The Carica and Vasconcellea sex chromosomes may have originated from the same autosomes bearing the X allelic form that still exist in the monoecious species V. monoica, and have evolved independently after the speciation event that separated Carica from Vasconcellea. Within Vasconcellea, sex chromosomes have evolved at the species level, at least for some species.
[Mh] Termos MeSH primário: Carica/genética
Caricaceae/genética
Cromossomos de Plantas/genética
Recombinação Genética/genética
[Mh] Termos MeSH secundário: Alelos
Teorema de Bayes
Evolução Molecular
Filogenia
Proteínas de Plantas/classificação
Proteínas de Plantas/genética
Polimorfismo de Nucleotídeo Único
Reprodução/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1103
[Cu] Atualização por classe:101105
[Lr] Data última revisão:
101105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100629
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-313X.2010.04284.x



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