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  1 / 411 MEDLINE  
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[PMID]:27775172
[Au] Autor:Favre A; Widmer A; Karrenberg S
[Ad] Endereço:Plant Ecological Genetics, ETH Zurich, Institute of Integrative Biology, Universitätstrasse 16, 8092, Zurich, Switzerland.
[Ti] Título:Differential adaptation drives ecological speciation in campions (Silene): evidence from a multi-site transplant experiment.
[So] Source:New Phytol;213(3):1487-1499, 2017 Feb.
[Is] ISSN:1469-8137
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In order to investigate the role of differential adaptation for the evolution of reproductive barriers, we conducted a multi-site transplant experiment with the dioecious sister species Silene dioica and S. latifolia and their hybrids. Crosses within species as well as reciprocal first-generation (F ) and second-generation (F ) interspecific hybrids were transplanted into six sites, three within each species' habitat. Survival and flowering were recorded over 4 yr. At all transplant sites, the local species outperformed the foreign species, reciprocal F hybrids performed intermediately and F hybrids underperformed in comparison to F hybrids (hybrid breakdown). Females generally had slightly higher cumulative fitness than males in both within- and between-species crosses and we thus found little evidence for Haldane's rule acting on field performance. The strength of selection against F and F hybrids as well as hybrid breakdown increased with increasing strength of habitat adaptation (i.e. the relative fitness difference between the local and the foreign species) across sites. Our results suggest that differential habitat adaptation led to ecologically dependent post-zygotic reproductive barriers and drives divergence and speciation in this Silene system.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Ecossistema
Especiação Genética
Silene/genética
Silene/fisiologia
[Mh] Termos MeSH secundário: Cruzamentos Genéticos
Flores/fisiologia
Aptidão Genética
Hibridização Genética
Modelos Biológicos
Razão de Masculinidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1111/nph.14202


  2 / 411 MEDLINE  
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[PMID]:28817728
[Au] Autor:Kolousková P; Stone JD; Storchová H
[Ad] Endereço:Plant Reproduction Laboratory, Institute of Experimental Botany v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic.
[Ti] Título:Evaluation of reference genes for reverse transcription quantitative real-time PCR (RT-qPCR) studies in Silene vulgaris considering the method of cDNA preparation.
[So] Source:PLoS One;12(8):e0183470, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Accurate gene expression measurements are essential in studies of both crop and wild plants. Reverse transcription quantitative real-time PCR (RT-qPCR) has become a preferred tool for gene expression estimation. A selection of suitable reference genes for the normalization of transcript levels is an essential prerequisite of accurate RT-qPCR results. We evaluated the expression stability of eight candidate reference genes across roots, leaves, flower buds and pollen of Silene vulgaris (bladder campion), a model plant for the study of gynodioecy. As random priming of cDNA is recommended for the study of organellar transcripts and poly(A) selection is indicated for nuclear transcripts, we estimated gene expression with both random-primed and oligo(dT)-primed cDNA. Accordingly, we determined reference genes that perform well with oligo(dT)- and random-primed cDNA, making it possible to estimate levels of nucleus-derived transcripts in the same cDNA samples as used for organellar transcripts, a key benefit in studies of cyto-nuclear interactions. Gene expression variance was estimated by RefFinder, which integrates four different analytical tools. The SvACT and SvGAPDH genes were the most stable candidates across various organs of S. vulgaris, regardless of whether pollen was included or not.
[Mh] Termos MeSH primário: DNA Complementar/genética
Genes de Plantas
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Silene/genética
[Mh] Termos MeSH secundário: Perfilação da Expressão Gênica
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (DNA, Complementary)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183470


  3 / 411 MEDLINE  
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[PMID]:28792972
[Au] Autor:Kawamoto H; Hirata A; Kawano S
[Ad] Endereço:Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Kashiwa, Chiba, Japan.
[Ti] Título:Three-dimensional ultrastructural study of the anther of Silene latifolia infected with Microbotryum lychnidis-dioicae.
[So] Source:PLoS One;12(8):e0182686, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:When Microbotryum lychnidis-dioicae infects a male Silene latifolia, M. lychnidis-dioicae smut spores develop in the pollen sac instead of pollen. In contrast, when M. lychnidis-dioicae infects a female S. latifolia, the female flowers become male-like, promoting stamen formation. However, it is unclear when and how M. lychnidis-dioicae invades the anther. It is important to investigate not only whether hyphae exist when the apical meristem tissue differentiates into flowers and anthers, but also whether hyphae exist when stamen filaments form. We used Grocott's methenamine silver stain and lectin stain, which stain chitin in the fungal cell wall, to search for M. lychnidis-dioicae in flower tissues. A few M. lychnidis-dioicae hyphae were observed intercellularly in the center of the connective of vascular bundles at the early anther developmental stage. Subsequently, large numbers of deeply stained M. lychnidis-dioicae hyphae were observed intercellularly in the cells surrounding the pollen sac, as well as in the center of the pollen sac. Hyphae stained with lectin were observed intercellularly in all of the stamen filaments at flower development stages. Hyphae were observed in the peduncle connecting the flower and stem. It is thought that M. lychnidis-dioicae invaded the anther via the stamen filament over a long period. Additionally, in total, 163 sections of connective were obtained, and the cell structure of each anther was colored and subjected to three-dimensional reconstruction. The M. lychnidis-dioicae hyphae observed in the connective were mainly old hyphae with large vacuoles or dead hyphae (S1 Fig). These hyphae branched out, towards the pollen sac, while growing between the cells. We also observed that the host cells that collapsed near the hyphae had thick cell walls and teliospores. Cell wall collapse and cell degeneration were observed only around hyphae with thick cell walls.
[Mh] Termos MeSH primário: Basidiomycota/ultraestrutura
Flores/ultraestrutura
Doenças das Plantas/microbiologia
Silene/ultraestrutura
[Mh] Termos MeSH secundário: Imagem Tridimensional
Microscopia
Microscopia Eletrônica de Transmissão
Silene/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182686


  4 / 411 MEDLINE  
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[PMID]:28645029
[Au] Autor:García-Gonzalo P; Pradas Del Real AE; Pirredda M; Gismera MJ; Lobo MC; Pérez-Sanz A
[Ad] Endereço:Department of Agro-Environmental Research, IMIDRA, Alcalá de Henares 28800, Madrid, Spain. Electronic address: pilar.garcia.gonzalo@madrid.org.
[Ti] Título:Phytoavailability of Cr in Silene vulgaris: The role of soil, plant genotype and bacterial rhizobiome.
[So] Source:Ecotoxicol Environ Saf;144:283-290, 2017 Oct.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Understanding the metal behavior at the soil-root interface is of utmost significance for a successful implementation of phytoremediation. In this study, we investigated the differences in chromium (Cr) uptake, chemical changes in soil solution and the shifts in rhizosphere bacterial communities of two genotypes of Silene vulgaris (SV21, SV38) with different tolerance to Cr. A greenhouse experiment was performed in two soils that differed on pH and organic matter (OM) content. An industrial sludge with high content in Cr was used as pollution source. The soil solution in the rhizosphere was sample by Rhizon Soil Moisture Samplers. The total concentration of Cr reached the highest values in soil solution samplers from calcareous soils with poor contents in OM. Plants grown in this soil also increased the Cr uptake in roots of both genotypes, but the concentration was higher in genotype SV-38 than in SV21. The clustering analysis of denaturing gradient gel electrophoresis (DGGE) of 16S rRNA fragments revealed major differences in bacterial community structure related to Cr pollution, followed by soil type and finally, plant genotype. Diversity indices based on DGGE profiles were the highest in alkaline soil, and between genotypes, values were significantly greater in SV38. Canonical correspondence analysis (CCA) showed that changes in bacterial community structure of rhizosphere were highly correlated with total Cr concentration and soil solution pH. The isolation and identification of S. vulgaris bacterial rhizosphere revealed a different composition according to soil type and plant genotype. Results suggested the potential role of Pseudomonas fluorescens on Cr mobilization and therefore, on enhanced metal bioavailability and may provide a starting point for further studies aimed at the combined use of tolerant plants and selected metal mobilizing rhizobacteria, in the microbial-assisted phytoremediation of Cr-polluted soils.
[Mh] Termos MeSH primário: Cromo/metabolismo
Rizosfera
Silene/genética
Microbiologia do Solo
Poluentes do Solo/metabolismo
Solo/química
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Eletroforese em Gel de Gradiente Desnaturante
Genótipo
Raízes de Plantas/metabolismo
Pseudomonas fluorescens/isolamento & purificação
RNA Ribossômico 16S/genética
Silene/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (Soil); 0 (Soil Pollutants); 0R0008Q3JB (Chromium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE


  5 / 411 MEDLINE  
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[PMID]:28241056
[Au] Autor:Kang JS; Lee BY; Kwak M
[Ad] Endereço:Plant Resources Division, National Institute of Biological Resources, Incheon, Republic of Korea.
[Ti] Título:The complete chloroplast genome sequences of Lychnis wilfordii and Silene capitata and comparative analyses with other Caryophyllaceae genomes.
[So] Source:PLoS One;12(2):e0172924, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The complete chloroplast genomes of Lychnis wilfordii and Silene capitata were determined and compared with ten previously reported Caryophyllaceae chloroplast genomes. The chloroplast genome sequences of L. wilfordii and S. capitata contain 152,320 bp and 150,224 bp, respectively. The gene contents and orders among 12 Caryophyllaceae species are consistent, but several microstructural changes have occurred. Expansion of the inverted repeat (IR) regions at the large single copy (LSC)/IRb and small single copy (SSC)/IR boundaries led to partial or entire gene duplications. Additionally, rearrangements of the LSC region were caused by gene inversions and/or transpositions. The 18 kb inversions, which occurred three times in different lineages of tribe Sileneae, were thought to be facilitated by the intermolecular duplicated sequences. Sequence analyses of the L. wilfordii and S. capitata genomes revealed 39 and 43 repeats, respectively, including forward, palindromic, and reverse repeats. In addition, a total of 67 and 56 simple sequence repeats were discovered in the L. wilfordii and S. capitata chloroplast genomes, respectively. Finally, we constructed phylogenetic trees of the 12 Caryophyllaceae species and two Amaranthaceae species based on 73 protein-coding genes using both maximum parsimony and likelihood methods.
[Mh] Termos MeSH primário: Caryophyllaceae/genética
DNA de Cloroplastos/genética
Genoma de Cloroplastos
Lychnis/genética
Silene/genética
[Mh] Termos MeSH secundário: DNA de Plantas
Evolução Molecular
Duplicação Gênica
Rearranjo Gênico
Genoma de Planta
Funções Verossimilhança
Repetições de Microssatélites/genética
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast); 0 (DNA, Plant)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172924


  6 / 411 MEDLINE  
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[PMID]:28050761
[Au] Autor:Pradas Del Real AE; Silvan JM; de Pascual-Teresa S; Guerrero A; García-Gonzalo P; Lobo MC; Pérez-Sanz A
[Ad] Endereço:Dpto. de Investigación Agroambiental, IMIDRA, Alcalá de Henares, 28800, Madrid, Spain. ana.pradas@ujf-grenoble.fr.
[Ti] Título:Role of the polycarboxylic compounds in the response of Silene vulgaris to chromium.
[So] Source:Environ Sci Pollut Res Int;24(6):5746-5756, 2017 Feb.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This work aims to investigate the nature and the specific mechanisms by which polycarboxylic compounds participate in the tolerance of Silene vulgaris to Cr with special attention given to the rhizosphere system. This knowledge is important to use this species in the implementation of phytoremediation technologies in Cr-polluted soils. According to the results, chromium is chelated and mobilized by the citric and malic acids in plant tissues, while oxalic acid might participate in the reduction and chelation of Cr in the rhizosphere. At the applied doses, the response of both exudation rate and root exudate composition (total polyphenols and quercitin) seems to involve a rearrangement in the lignification of the plant cell wall to immobilize Cr. Quercetin-3-dirhamnosyl-galactoside and apiin (apigenin-7-O-apiosyl-glucoside) have been identified as the major polyphenols in the root exudates of S. vulgaris. The increments found in the apiin concentration in root exudates seem to be related to the protection against Cr toxicity by chelation of Cr or by free radical scavenging. Though earlier response is detected in plant tissues, results from this work together with previous studies in S. vulgaris indicate that exudation might be a regulated mechanism of protection under Cr exposition in S. vulgaris that may involve mainly Cr reduction and chelation.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Cromo
Silene
[Mh] Termos MeSH secundário: Ácido Cítrico
Malatos
Ácido Oxálico
Raízes de Plantas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Malates); 0R0008Q3JB (Chromium); 2968PHW8QP (Citric Acid); 817L1N4CKP (malic acid); 9E7R5L6H31 (Oxalic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170105
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-8218-4


  7 / 411 MEDLINE  
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[PMID]:28011871
[Au] Autor:Kawamoto H; Yamanaka K; Koizumi A; Hirata A; Kawano S
[Ad] Endereço:Department of Integrated Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan.
[Ti] Título:Cell Death and Cell Cycle Arrest of Silene latifolia Stamens and Pistils After Microbotryum lychnidis-dioicae Infection.
[So] Source:Plant Cell Physiol;58(2):320-328, 2017 02 01.
[Is] ISSN:1471-9053
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Mechanisms of suppression of pistil primordia in male flowers and of stamen primordia in female flowers differ in diclinous plants. In this study, we investigated how cell death and cell cycle arrest are related to flower organ formation in Silene latifolia. Using in situ hybridization and a TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay, we detected both cell cycle arrest and cell death in suppressed stamens of female flowers and suppressed pistils of male flowers in S. latifolia. In female flowers infected with Microbotryum lychnidis-dioicae, developmental suppression of stamens is released, and cell cycle arrest and cell death do not occur. Smut spores are formed in S. latifolia anthers infected with M. lychnidis-dioicae, followed by cell death in the endothelium, middle layer, tapetal cells and pollen mother cells. Cell death is difficult to detect using a fluorescein isothiocyanate-labeled TUNEL assay due to strong autofluorescence in the anther. We therefore combined a TUNEL assay in an infrared region with transmission electron microscopy to detect cell death in anthers. We show that following infection by M. lychnidis-dioicae, a TUNEL signal was not detected in the endothelium, middle layer or pollen mother cells, and cell death with outflow of cell contents, including the nucleoplast, was observed in tapetal cells.
[Mh] Termos MeSH primário: Basidiomycota/fisiologia
Flores/metabolismo
Silene/metabolismo
Silene/microbiologia
[Mh] Termos MeSH secundário: Pontos de Checagem do Ciclo Celular/fisiologia
Morte Celular/fisiologia
Flores/microbiologia
Pólen/metabolismo
Pólen/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170723
[Lr] Data última revisão:
170723
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161225
[St] Status:MEDLINE
[do] DOI:10.1093/pcp/pcw193


  8 / 411 MEDLINE  
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[PMID]:27827389
[Au] Autor:Campos JL; Qiu S; Guirao-Rico S; Bergero R; Charlesworth D
[Ad] Endereço:Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
[Ti] Título:Recombination changes at the boundaries of fully and partially sex-linked regions between closely related Silene species pairs.
[So] Source:Heredity (Edinb);118(4):395-403, 2017 Apr.
[Is] ISSN:1365-2540
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The establishment of a region of suppressed recombination is a critical change during sex chromosome evolution, leading to such properties as Y (and W) chromosome genetic degeneration, accumulation of repetitive sequences and heteromorphism. Although chromosome inversions can cause large regions to have suppressed recombination, and inversions are sometimes involved in sex chromosome evolution, gradual expansion of the non-recombining region could potentially sometimes occur. We here test whether closer linkage has recently evolved between the sex-determining region and several genes that are partially sex-linked in Silene latifolia, using Silene dioica, a closely related dioecious plants whose XY sex chromosome system is inherited from a common ancestor. The S. latifolia pseudoautosomal region (PAR) includes several genes extremely closely linked to the fully Y-linked region. These genes were added to an ancestral PAR of the sex chromosome pair in two distinct events probably involving translocations of autosomal genome regions causing multiple genes to become partially sex-linked. Close linkage with the PAR boundary must have evolved since these additions, because some genes added in both events now show almost complete sex linkage in S. latifolia. We compared diversity patterns of five such S. latifolia PAR boundary genes with their orthologues in S. dioica, including all three regions of the PAR (one gene that was in the ancestral PAR and two from each of the added regions). The results suggest recent recombination suppression in S. latifolia, since its split from S. dioica.
[Mh] Termos MeSH primário: Ligação Genética
Recombinação Genética
Cromossomos Sexuais/genética
Silene/genética
[Mh] Termos MeSH secundário: Cromossomos de Plantas/genética
Evolução Molecular
Genes de Plantas
Genética Populacional/métodos
Polimorfismo de Nucleotídeo Único
Sequências Repetitivas de Ácido Nucleico
Silene/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE
[do] DOI:10.1038/hdy.2016.113


  9 / 411 MEDLINE  
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[PMID]:27870888
[Au] Autor:Casazza G; Grassi F; Zecca G; Minuto L
[Ad] Endereço:DISTAV, Università degli studi di Genova, Genova, Italy.
[Ti] Título:Phylogeographic Insights into a Peripheral Refugium: The Importance of Cumulative Effect of Glaciation on the Genetic Structure of Two Endemic Plants.
[So] Source:PLoS One;11(11):e0166983, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quaternary glaciations and mostly last glacial maximum have shaped the contemporary distribution of many species in the Alps. However, in the Maritime and Ligurian Alps a more complex picture is suggested by the presence of many Tertiary paleoendemisms and by the divergence time between lineages in one endemic species predating the Late Pleistocene glaciation. The low number of endemic species studied limits the understanding of the processes that took place within this region. We used species distribution models and phylogeographical methods to infer glacial refugia and to reconstruct the phylogeographical pattern of Silene cordifolia All. and Viola argenteria Moraldo & Forneris. The predicted suitable area for last glacial maximum roughly fitted current known distribution. Our results suggest that separation of the major clades predates the last glacial maximum and the following repeated glacial and interglacial periods probably drove differentiations. The complex phylogeographical pattern observed in the study species suggests that both populations and genotypes extinction was minimal during the last glacial maximum, probably due to the low impact of glaciations and to topographic complexity in this area. This study underlines the importance of cumulative effect of previous glacial cycles in shaping the genetic structure of plant species in Maritime and Ligurian Alps, as expected for a Mediterranean mountain region more than for an Alpine region.
[Mh] Termos MeSH primário: Camada de Gelo
Silene/genética
Viola/genética
[Mh] Termos MeSH secundário: Filogeografia
Silene/crescimento & desenvolvimento
Viola/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161122
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166983


  10 / 411 MEDLINE  
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[PMID]:27832131
[Au] Autor:Fortuna TM; Snirc A; Badouin H; Gouzy J; Siguenza S; Esquerre D; Le Prieur S; Shykoff JA; Giraud T
[Ad] Endereço:Laboratoire d'Ecologie Systématique Evolution, Univ. Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, 91400, Orsay, France.
[Ti] Título:Polymorphic Microsatellite Markers for the Tetrapolar Anther-Smut Fungus Microbotryum saponariae Based on Genome Sequencing.
[So] Source:PLoS One;11(11):e0165656, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Anther-smut fungi belonging to the genus Microbotryum sterilize their host plants by aborting ovaries and replacing pollen by fungal spores. Sibling Microbotryum species are highly specialized on their host plants and they have been widely used as models for studies of ecology and evolution of plant pathogenic fungi. However, most studies have focused, so far, on M. lychnidis-dioicae that parasitizes the white campion Silene latifolia. Microbotryum saponariae, parasitizing mainly Saponaria officinalis, is an interesting anther-smut fungus, since it belongs to a tetrapolar lineage (i.e., with two independently segregating mating-type loci), while most of the anther-smut Microbotryum fungi are bipolar (i.e., with a single mating-type locus). Saponaria officinalis is a widespread long-lived perennial plant species with multiple flowering stems, which makes its anther-smut pathogen a good model for studying phylogeography and within-host multiple infections. PRINCIPAL FINDINGS: Here, based on a generated genome sequence of M. saponariae we developed 6 multiplexes with a total of 22 polymorphic microsatellite markers using an inexpensive and efficient method. We scored these markers in fungal individuals collected from 97 populations across Europe, and found that the number of their alleles ranged from 2 to 11, and their expected heterozygosity from 0.01 to 0.58. Cross-species amplification was examined using nine other Microbotryum species parasitizing hosts belonging to Silene, Dianthus and Knautia genera. All loci were successfully amplified in at least two other Microbotryum species. SIGNIFICANCE: These newly developed markers will provide insights into the population genetic structure and the occurrence of within-host multiple infections of M. saponariae. In addition, the draft genome of M. saponariae, as well as one of the described markers will be useful resources for studying the evolution of the breeding systems in the genus Microbotryum and the evolution of specialization onto different plant species.
[Mh] Termos MeSH primário: Basidiomycota/genética
Dianthus/microbiologia
Dipsacaceae/microbiologia
Repetições de Microssatélites
Silene/microbiologia
[Mh] Termos MeSH secundário: Basidiomycota/isolamento & purificação
Genoma Fúngico
Doenças das Plantas/microbiologia
Polimorfismo Genético
Ustilaginales
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0165656



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