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[PMID]: 29369560 [Au] Autor: Vetchinkina EM; Komakhina VV; Vysotskii DA; Zaitsev DV; Smirnov AN; Babakov AV; Komakhin RA [Ti] Título: [Expression of plant antimicrobial peptide pro-SmAMP2 gene increases resistance of transgenic potato plants to Alternaria and Fusarium pathogens]. [So] Source: Genetika;52(9):1055-68, 2016 Sep. [Is] ISSN: 0016-6758 [Cp] País de publicação: Russia (Federation) [La] Idioma: rus [Ab] Resumo: The chickweed (Stellaria media L.) pro-SmAMP2 gene encodes the hevein-like peptides that have in vitro antimicrobial activity against certain harmful microorganisms. These peptides play an important role in protecting the chickweed plants from infection, and the pro-SmAMP2 gene was previously used to protect transgenic tobacco and Arabidopsis plants from phytopathogens. In this study, the pro-SmAMP2 gene under control of viral CaMV35S promoter or under control of its own pro-SmAMP2 promoter was transformed into cultivated potato plants of two cultivars, differing in the resistance to Alternaria: Yubiley Zhukova (resistant) and Skoroplodny (susceptible). With the help of quantitative real-time PCR, it was demonstrated that transgenic potato plants expressed the pro-SmAMP2 gene under control of both promoters at the level comparable to or exceeding the level of the potato actin gene. Assessment of the immune status of the transformants demonstrated that expression of antimicrobial peptide pro-SmAMP2 gene was able to increase the resistance to a complex of Alternaria sp. and Fusarium sp. phytopathogens only in potato plants of the Yubiley Zhukova cultivar. The possible role of the pro-SmAMP2 products in protecting potatoes from Alternaria sp. and Fusarium sp. is discussed. [Mh] Termos MeSH primário: Alternaria Peptídeos Catiônicos Antimicrobianos Resistência à Doença/genética Fusarium Proteínas de Plantas Plantas Geneticamente Modificadas Solanum tuberosum [Mh] Termos MeSH secundário: Peptídeos Catiônicos Antimicrobianos/biossíntese Peptídeos Catiônicos Antimicrobianos/genética Proteínas de Plantas/biossíntese Proteínas de Plantas/genética Plantas Geneticamente Modificadas/genética Plantas Geneticamente Modificadas/metabolismo Plantas Geneticamente Modificadas/microbiologia Solanum tuberosum/genética Solanum tuberosum/metabolismo Solanum tuberosum/microbiologia Stellaria/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antimicrobial Cationic Peptides); 0 (Plant Proteins) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180205 [Lr] Data última revisão: 180205 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180126 [St] Status: MEDLINE

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[PMID]: 28038935 [Au] Autor: Slavokhotova AA; Shelenkov AA; Korostyleva TV; Rogozhin EA; Melnikova NV; Kudryavtseva AV; Odintsova TI [Ad] Endereço: Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina Str., 119991 Moscow, Russian Federation. Electronic address: annslav82@gmail.com. [Ti] Título: Defense peptide repertoire of Stellaria media predicted by high throughput next generation sequencing. [So] Source: Biochimie;135:15-27, 2017 Apr. [Is] ISSN: 1638-6183 [Cp] País de publicação: France [La] Idioma: eng [Ab] Resumo: Being perfectly adapted to diverse environments, chickweed (Stellaria media (L.) Vill), a ubiquitous garden weed, grows widely in Europe and North America. As opposed to the model plants, many weeds, and S. media in particular, have been poorly studied, although they are likely to contain promising components of immunity and novel resistance genes. In this study, for the first time RNA-seq analysis of healthy and infected with Fusarium oxysporum chickweed seedlings, as well as de novo transcriptome assembly and annotation, are presented. Note, this research is focused on antimicrobial peptides (AMPs), the major components of plant immune system. Using custom software developed earlier, 145 unique putative AMPs (pAMPs) including defensins, thionins, hevein-like peptides, snakins, alpha-hairpinins, LTPs, and cysteine-rich peptides with novel cysteine motifs were predicted. Furthermore, changes in AMP expression profile in response to fungal infection were traced. In addition, the comparison of chickweed AMP repertoire with those of other Caryophyllaceae plants whose transcriptomes are presently available is made. As a result, alpha-hairpinins and hevein-like peptides which display characteristic modular structure appear to be specific AMPs distinguishing S. media from Dianthus caryophyllus, Silene vulgaris, and Silene latifolia. Finally, revealing several AMPs with proven antimicrobial activity gives opportunity to conclude that the presented method of AMP repertoire analysis reveals highly active AMPs playing vital role in plant immunity. [Mh] Termos MeSH primário: Sequenciamento de Nucleotídeos em Larga Escala/métodos Stellaria/metabolismo [Mh] Termos MeSH secundário: Peptídeos Catiônicos Antimicrobianos/metabolismo Regulação da Expressão Gênica de Plantas Imunidade Vegetal Proteínas de Plantas/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antimicrobial Cationic Peptides); 0 (Plant Proteins) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170321 [Lr] Data última revisão: 170321 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170101 [St] Status: MEDLINE

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[PMID]: 27189173 [Au] Autor: Komakhin RA; Vysotskii DA; Shukurov RR; Voblikova VD; Komakhina VV; Strelnikova SR; Vetchinkina EM; Babakov AV [Ad] Endereço: All-Russia Research Institute of Agricultural Biotechnology, Timiriazevskaya 42, 127550, Moscow, Russia. recombination@iab.ac.ru. [Ti] Título: Novel strong promoter of antimicrobial peptides gene pro-SmAMP2 from chickweed (Stellaria media). [So] Source: BMC Biotechnol;16(1):43, 2016 May 18. [Is] ISSN: 1472-6750 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: In a previous study we found that in chickweed the expression level of the pro-SmAMP2 gene was comparable or even higher to that of the ß-actin gene. This high level of the gene expression has attracted our attention as an opportunity for the identification of novel strong promoters of plant origin, which could find its application in plant biotechnology. Therefore, in the present study we focused on the nucleotide sequence identification and the functional characteristics of the pro-SmAMP2 promoter in transgenic plants. RESULTS: In chickweed (Stellaria media), a 2120 bp promoter region of the pro-SmAMP2 gene encoding antifungal peptides was sequenced. Six 5'-deletion variants -2120, -1504, -1149, -822, -455, and -290 bp of pro-SmAMP2 gene promoter were fused with the coding region of the reporter gene gusA in the plant expression vector pCambia1381Z. Independent transgenic plants of tobacco Nicotiana tabacum were obtained with each genetic structure. GUS protein activity assay in extracts from transgenic plants showed that all deletion variants of the promoter, except -290 bp, expressed the gusA gene. In most transgenic plants, the GUS activity level was comparable or higher than in plants with the viral promoter CaMV 35S. GUS activity remains high in progenies and its level correlates positively with the amount of gusA gene mRNA in T3 homozygous plants. The activity of the Ñ€ro-SmAMP2 promoter was detected in all organs of the transgenic plants studied, during meiosis and in pollen as well. CONCLUSION: Our results show that the Ñ€ro-SmAMP2 promoter can be used for target genes expression control in transgenic plants. [Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/genética Regulação da Expressão Gênica de Plantas/genética Proteínas de Plantas/genética Plantas Geneticamente Modificadas/genética Regiões Promotoras Genéticas/genética Stellaria/genética [Mh] Termos MeSH secundário: Sequência de Bases Dados de Sequência Molecular [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antimicrobial Cationic Peptides); 0 (Plant Proteins) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170623 [Lr] Data última revisão: 170623 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160519 [St] Status: MEDLINE [do] DOI: 10.1186/s12896-016-0273-x

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[PMID]: 26196691 [Au] Autor: Rogozhin EA; Slezina MP; Slavokhotova AA; Istomina EA; Korostyleva TV; Smirnov AN; Grishin EV; Egorov TA; Odintsova TI [Ad] Endereço: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation. Electronic address: rea21@list.ru. [Ti] Título: A novel antifungal peptide from leaves of the weed Stellaria media L. [So] Source: Biochimie;116:125-32, 2015 Sep. [Is] ISSN: 1638-6183 [Cp] País de publicação: France [La] Idioma: eng [Ab] Resumo: A novel peptide named SmAMP3 was isolated from leaves of common chickweed (Stellaria media L.) by a combination of acidic extraction and a single-step reversed-phase HPLC and sequenced. The peptide is basic and cysteine-rich, consists of 35 amino acids, and contains three disulphide bridges. Homology search revealed that SmAMP3 belongs to the family of hevein-like antimicrobial peptides carrying a conserved chitin-binding site. Efficient binding of chitin by SmAMP3 was proved by in vitro assays. Molecular modeling confirmed conservation of the chitin-binding module in SmAMP3 locating the variable amino acid residues to the solvent-exposed loops of the molecule. The peptide exhibits potent antifungal activity against important plant pathogens in the micromolar range, although it is devoid of antibacterial activity at concentrations below 10 µM. As judged by chromatographic behavior and mass spectrometric data, the peptide is constitutively expressed in above-ground organs and seeds of S. media plants, thus representing an important player in the preformed branch of the plant immune system. [Mh] Termos MeSH primário: Antifúngicos/química Folhas de Planta/química Stellaria/química [Mh] Termos MeSH secundário: Anti-Infecciosos/química Anti-Infecciosos/farmacologia Antifúngicos/farmacologia Quitina/metabolismo Testes de Sensibilidade Microbiana Peptídeos/química Peptídeos/farmacologia Proteínas de Plantas/química Proteínas de Plantas/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Infective Agents); 0 (Antifungal Agents); 0 (Peptides); 0 (Plant Proteins); 1398-61-4 (Chitin) [Em] Mês de entrada: 1605 [Cu] Atualização por classe: 150810 [Lr] Data última revisão: 150810 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150722 [St] Status: MEDLINE

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[PMID]: 26113156 [Au] Autor: Kurepin LV; Pharis RP; Neil Emery RJ; Reid DM; Chinnappa CC [Ad] Endereço: Department of Biology, Western University, London, Ontario, Canada. Electronic address: lkurepin@uwo.ca. [Ti] Título: Phenotypic plasticity of sun and shade ecotypes of Stellaria longipes in response to light quality signaling, gibberellins and auxin. [So] Source: Plant Physiol Biochem;94:174-80, 2015 Sep. [Is] ISSN: 1873-2690 [Cp] País de publicação: France [La] Idioma: eng [Ab] Resumo: Stellaria longipes plant communities (ecotypes) occur in several environmentally distinct habitats along the eastern slopes of southern Alberta's Rocky Mountains. One ecotype occurs in a prairie habitat at ∼1000 m elevation where Stellaria plants grow in an environment in which the light is filtered by taller neighbouring vegetation, i.e. sunlight with a low red to far-red (R/FR) ratio. This ecotype exhibits a high degree of phenotypic plasticity by increasing stem elongation in response to the low R/FR ratio light signal. Another Stellaria ecotype occurs nearby at ∼2400 m elevation in a much cooler alpine habitat, one where plants rarely experience low R/FR ratio shade light. Stem elongation of plants is largely regulated by gibberellins (GAs) and auxin, indole-3-acetic acid (IAA). Shoots of the prairie ecotype plants show increased IAA levels under low R/FR ratio light and they also increase their stem growth in response to applied IAA. The alpine ecotype plants show neither response. Plants from both ecotypes produce high levels of growth-active GA1 under low R/FR ratio light, though they differ appreciably in their catabolism of GA1. The alpine ecotype plants exhibit very high levels of GA8, the inactive product of GA1 metabolism, under both normal and low R/FR ratio light. Alpine origin plants may de-activate GA1 by conversion to GA8 via a constitutively high level of expression of the GA2ox gene, thereby maintaining their dwarf phenotype and exhibiting a reduced phenotypic plasticity in terms of shoot elongation. In contrast, prairie plants exhibit a high degree of phenotypic plasticity, using low R/FR ratio light-mediated changes in GA and IAA concentrations to increase shoot elongation, thereby accessing direct sunlight to optimize photosynthesis. There thus appear to be complex adaptation strategies for the two ecotypes, ones which involve modifications in the homeostasis of endogenous hormones. [Mh] Termos MeSH primário: Giberelinas/metabolismo Ácidos Indolacéticos/metabolismo Fenótipo Transdução de Sinais/fisiologia Stellaria/crescimento & desenvolvimento Luz Solar [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Gibberellins); 0 (Indoleacetic Acids) [Em] Mês de entrada: 1604 [Cu] Atualização por classe: 161020 [Lr] Data última revisão: 161020 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150627 [St] Status: MEDLINE

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[PMID]: 25924523 [Au] Autor: Ancheeva E; Daletos G; Muharini R; Lin WH; Teslov L; Proksch P [Ti] Título: Flavonoids from Stellaria nemorum and Stellaria holostea. [So] Source: Nat Prod Commun;10(3):437-40, 2015 Mar. [Is] ISSN: 1934-578X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Stellaria nemorum L. and S. holostea L. (Caryophyllaceae) were investigated for their flavonoids. The new flavonoid 6-C-[(α-arabinopyranosyl)-( 1-->2)-O-ß- xylopyranosyl]apigenin (1) and the four known C-glycosides, 6-C-[(α-arabinopyranosyl)-(1-->2)-O-ß-glucopyranosyl]apigenin (2), apigenin 6-C-ß- galactopyranoside-8-C-ß-glucopyranoside (3), apigenin 6-C-ß-glucopyranoside-8-C-α-arabinopyranoside (4), and apigenin 6-C-ß-glucopyranoside-8-C-ß- xylopyranoside (5) were isolated from the aerial parts of S. nemorum for the first time. Furthemore, five known flavonoids, 3,5,7-trihydroxy-3',5'- dimethoxyflavone (9), diosmetin 6-C-ß-glucopyranoside (8), schaftoside (4), isoorientin (6) and orientin (7) were obtained from the aerial parts of S. holostea. Compounds 4, 8 and 9 are reported for the first time from this species. The structures of all isolated compounds were unambiguously elucidated by one- and two- dimensional NMR and mass spectral analysis, by acid hydrolysis, as well as by comparison with literature data. The crude extracts of the investigated species exhibited antimicrobial activity against Staphylococcus aureus, while none of the isolated compounds was found to be active. [Mh] Termos MeSH primário: Flavonoides/química Stellaria/química [Mh] Termos MeSH secundário: Estrutura Molecular Especificidade da Espécie [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Flavonoids) [Em] Mês de entrada: 1506 [Cu] Atualização por classe: 150430 [Lr] Data última revisão: 150430 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150501 [St] Status: MEDLINE

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[PMID]: 25272829 [Au] Autor: Lang DY; Cui JJ; Da Z; Li YT; Zhou L; Zhang XH [Ti] Título: [Effect of drought stress on growth and physiological-biochemical characteristics of Stellaria dichotoma]. [So] Source: Zhongguo Zhong Yao Za Zhi;39(11):1995-9, 2014 Jun. [Is] ISSN: 1001-5302 [Cp] País de publicação: China [La] Idioma: chi [Ab] Resumo: A pot experiment was conducted to study effect of drought stress on leaf physiological characteristics and growth of one year old Stellaria dichotoma seedlings. The result showed that plant height and shoot dry weight significantly decreased with decrease in soil water content; however, root length and root dry weight increased at light drought stress and decreased at severe drought stress. The result also showed that with the decrease of soil water content, proline content in S. dichotoma leaves decreased then increase, while solube protein content decreased. Activities of SOD and POD in S. dichotoma leaves significantly decreased as soil water content decreased, while activity of CAT significantly decreased at severe drought stress. Membrane permeability in S. dichotoma leaves increased, while MDA content decreased then increased as soil water decreased. These results suggest that S. dichotoma had osmotic stress resistance ability and reactive oxygen scavenging capacity at light drought stress, which caused S. dichotoma growth was no inhibited at a certain extent drought stress. [Mh] Termos MeSH primário: Stellaria/crescimento & desenvolvimento Água/metabolismo [Mh] Termos MeSH secundário: Secas Folhas de Planta/enzimologia Folhas de Planta/crescimento & desenvolvimento Folhas de Planta/metabolismo Proteínas de Plantas/metabolismo Raízes de Plantas/enzimologia Raízes de Plantas/crescimento & desenvolvimento Raízes de Plantas/metabolismo Prolina/metabolismo Plântulas/enzimologia Plântulas/crescimento & desenvolvimento Plântulas/metabolismo Stellaria/enzimologia Stellaria/metabolismo [Pt] Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Plant Proteins); 059QF0KO0R (Water); 9DLQ4CIU6V (Proline) [Em] Mês de entrada: 1410 [Cu] Atualização por classe: 151119 [Lr] Data última revisão: 151119 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 141003 [St] Status: MEDLINE

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[PMID]: 24081691 [Au] Autor: Slavokhotova AA; Rogozhin EA; Musolyamov AK; Andreev YA; Oparin PB; Berkut AA; Vassilevski AA; Egorov TA; Grishin EV; Odintsova TI [Ad] Endereço: Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, 119991, Moscow, Russian Federation, annslav82@gmail.com. [Ti] Título: Novel antifungal α-hairpinin peptide from Stellaria media seeds: structure, biosynthesis, gene structure and evolution. [So] Source: Plant Mol Biol;84(1-2):189-202, 2014 Jan. [Is] ISSN: 1573-5028 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Plant defense against disease is a complex multistage system involving initial recognition of the invading pathogen, signal transduction and activation of specialized genes. An important role in pathogen deterrence belongs to so-called plant defense peptides, small polypeptide molecules that present antimicrobial properties. Using multidimensional liquid chromatography, we isolated a novel antifungal peptide named Sm-AMP-X (33 residues) from the common chickweed (Stellaria media) seeds. The peptide sequence shows no homology to any previously described proteins. The peculiar cysteine arrangement (C(1)X3C(2)XnC(3)X3C(4)), however, allocates Sm-AMP-X to the recently acknowledged α-hairpinin family of plant defense peptides that share the helix-loop-helix fold stabilized by two disulfide bridges C(1)-C(4) and C(2)-C(3). Sm-AMP-X exhibits high broad-spectrum activity against fungal phytopathogens. We further showed that the N- and C-terminal "tail" regions of the peptide are important for both its structure and activity. The truncated variants Sm-AMP-X1 with both disulfide bonds preserved and Sm-AMP-X2 with only the internal S-S-bond left were progressively less active against fungi and presented largely disordered structure as opposed to the predominantly helical conformation of the full-length antifungal peptide. cDNA and gene cloning revealed that Sm-AMP-X is processed from a unique multimodular precursor protein that contains as many as 12 tandem repeats of α-hairpinin-like peptides. Structure of the sm-amp-x gene and two related pseudogenes sm-amp-x-ψ1 and sm-amp-x-ψ2 allows tracing the evolutionary scenario that led to generation of such a sophisticated precursor protein. Sm-AMP-X is a new promising candidate for engineering disease resistance in plants. [Mh] Termos MeSH primário: Antifúngicos/metabolismo Proteínas de Plantas/biossíntese Proteínas de Plantas/metabolismo Sementes/metabolismo Stellaria/química [Mh] Termos MeSH secundário: Sequência de Aminoácidos Antifúngicos/química Clonagem Molecular Evolução Molecular Fungos/efeitos dos fármacos Regulação da Expressão Gênica de Plantas/fisiologia Dados de Sequência Molecular Proteínas de Plantas/química Proteínas de Plantas/genética Sementes/química Sementes/genética Stellaria/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Antifungal Agents); 0 (Plant Proteins) [Em] Mês de entrada: 1401 [Cu] Atualização por classe: 171031 [Lr] Data última revisão: 171031 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 131002 [St] Status: MEDLINE [do] DOI: 10.1007/s11103-013-0127-z

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[PMID]: 25149235 [Au] Autor: Liu W; Bi Y; Li L; Yuan G; Du L; Wang J [Ad] Endereço: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong, Tai'an 271018, PR China. [Ti] Título: Target-site basis for resistance to acetolactate synthase inhibitor in Water chickweed (Myosoton aquaticum L.). [So] Source: Pestic Biochem Physiol;107(1):50-4, 2013 Sep. [Is] ISSN: 1095-9939 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Water chickweed is a widespread and competitive winter annual or biennial weed of wheat in China. One Water chickweed population (HN02) resistant to several acetolactate synthase (ALS) inhibitors was found in Henan province of China. Whole-plant bioassays showed that HN02 was high resistance to tribenuron (292.05-flod). In vitro ALS assays revealed that resistance was due to reduced sensitivity of the ALS enzyme to tribenuron. The I50 value for HN02 was 85.53 times greater respectively than that of susceptible population (SD05). This altered ALS sensitivity in the resistant population was due to a mutation in the ALS gene resulting in a Pro197 to Ser substitution. Cross-resistance experiments indicated that HN02 exhibited various resistance patterns to pyrithiobac-sodium, florasulam and pyroxsulam, without resistance to imazethapyr. This is the first report of tribenuron-resistant Water chickweed in Henan province of China, target-site based resistance was established as being due to an insensitive form of ALS, resulting from a Pro to Ser substitution at amino acid position 197 in the ALS gene. [Mh] Termos MeSH primário: Acetolactato Sintase/antagonistas & inibidores Sulfonatos de Arila/farmacologia Herbicidas/farmacologia Proteínas de Plantas/antagonistas & inibidores Stellaria/efeitos dos fármacos [Mh] Termos MeSH secundário: Acetolactato Sintase/genética Substituição de Aminoácidos DNA de Plantas/genética Resistência a Herbicidas/genética Proteínas de Plantas/genética Prolina/genética Serina/genética Stellaria/enzimologia Stellaria/genética [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Arylsulfonates); 0 (DNA, Plant); 0 (Herbicides); 0 (Plant Proteins); 2589ET7417 (metsulfuron methyl); 452VLY9402 (Serine); 9DLQ4CIU6V (Proline); EC 2.2.1.6 (Acetolactate Synthase) [Em] Mês de entrada: 1605 [Cu] Atualização por classe: 161125 [Lr] Data última revisão: 161125 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140824 [St] Status: MEDLINE

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[PMID]: 23761431 [Au] Autor: Shan Y; Zheng Y; Guan F; Zhou J; Zhao H; Xia B; Feng X [Ad] Endereço: Jiangsu Center for Research & Development of Medicinal Plants, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden, Mem. Sun Yat-sen), Nanjing 210014, China. [Ti] Título: Purification and characterization of a novel anti-HSV-2 protein with antiproliferative and peroxidase activities from Stellaria media. [So] Source: Acta Biochim Biophys Sin (Shanghai);45(8):649-55, 2013 Aug. [Is] ISSN: 1745-7270 [Cp] País de publicação: China [La] Idioma: eng [Ab] Resumo: A novel antiviral protein, designated as Stellarmedin A, was purified from Stellaria media (L.) Vill. (Caryophyllaceae) by using ammonium sulfate precipitation, cation-exchange chromatography system. Gel electrophoresis analysis showed that Stellarmedin A is a highly basic glycoprotein with a molecular weight of 35.1 kDa and an isoelectric point of ∼8.7. The N-terminal 14-amino acid sequence, MGNTGVLTGERNDR, is similar to those of other plant peroxidases. This protein inhibited herpes simplex virus type 2 (HSV-2) replication in vitro with an IC50 of 13.18 µg/ml and a therapeutic index exceeding 75.9. It was demonstrated that Stellarmedin A affects the initial stage of HSV-2 infection and is able to inhibit the proliferation of promyelocytic leukemia HL-60 and colon carcinoma LoVo cells with an IC50 of 9.09 and 12.32 µM, respectively. Moreover, Stellarmedin A has a peroxidase activity of 36.6 µmol/min/mg protein, when guaiacol was used as substrate. To our knowledge, this is the first report about an anti-HSV-2 protein with antiproliferative and peroxidase activities from S. media. [Mh] Termos MeSH primário: Antivirais/isolamento & purificação Proliferação Celular/efeitos dos fármacos Herpesvirus Humano 2/efeitos dos fármacos Peroxidases/metabolismo Proteínas de Plantas/isolamento & purificação Stellaria/metabolismo [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Antivirais/química Antivirais/farmacologia Cercopithecus aethiops Dados de Sequência Molecular Peroxidases/química Peroxidases/isolamento & purificação Peroxidases/farmacologia Proteínas de Plantas/química Proteínas de Plantas/farmacologia Células Vero [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Antiviral Agents); 0 (Plant Proteins); EC 1.11.1.- (Peroxidases); EC 1.11.1.- (stellarmedin A protein, Stellaria media) [Em] Mês de entrada: 1403 [Cu] Atualização por classe: 130723 [Lr] Data última revisão: 130723 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 130614 [St] Status: MEDLINE [do] DOI: 10.1093/abbs/gmt060

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2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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