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[PMID]: | 28898278 |
[Au] Autor: | Chen Y; Zhi J; Zhang H; Li J; Zhao Q; Xu J |
[Ad] Endereço: | National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China. |
[Ti] Título: | Transcriptome analysis of Phytolacca americana L. in response to cadmium stress. |
[So] Source: | PLoS One;12(9):e0184681, 2017. | [Is] ISSN: | 1932-6203 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Phytolacca americana L. (pokeweed) has metal phytoremediation potential, but little is known about its metal accumulation-related genes. In this study, the de novo sequencing of total RNA produced 53.15 million reads covering 10.63 gigabases of transcriptome raw data in cadmium (Cd)-treated and untreated pokeweed. Of the 97,502 assembled unigenes, 42,197 had significant matches in a public database and were annotated accordingly. An expression level comparison between the samples revealed 1515 differentially expressed genes (DEGs), 923 down- and 592 up-regulated under Cd treatment. A KEGG pathway enrichment analysis of DEGs revealed that they were involved in 72 metabolism pathways, with photosynthesis, phenylalanine metabolism, ribosome, phenylpropanoid biosynthesis, flavonoid biosynthesis and carbon fixation in photosynthetic organisms containing 24, 18, 72, 14, 7 and 15 genes, respectively. Genes related to heavy metal tolerance, absorption, transport and accumulation were also identified, including 11 expansins, 8 nicotianamine synthases, 6 aquaporins, 4 ZRT/IRT-like proteins, 3 ABC transporters and 3 metallothioneins. The gene expression results of 12 randomly selected DEGs were validated using quantitative real-time PCR, and showed different response patterns to Cd in their roots, stems and leaves. These results may be helpful in increasing our understanding of heavy metal hyperaccumulators and in future phytoremediation applications. |
[Mh] Termos MeSH primário: |
Cádmio/toxicidade Phytolacca americana/genética Estresse Fisiológico Transcriptoma
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[Mh] Termos MeSH secundário: |
Phytolacca americana/efeitos dos fármacos Phytolacca americana/metabolismo Proteínas de Plantas/genética Proteínas de Plantas/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Plant Proteins); 00BH33GNGH (Cadmium) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171012 |
[Lr] Data última revisão:
| 171012 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170913 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1371/journal.pone.0184681 |
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