Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.198.500.875.500.500 [Categoria DeCS] Referências encontradas : 92 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 10

ir para página

1 / 92

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28898278 [Au] Autor: Chen Y; Zhi J; Zhang H; Li J; Zhao Q; Xu J [Ad] Endereço: National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China. [Ti] Título: Transcriptome analysis of Phytolacca americana L. in response to cadmium stress. [So] Source: PLoS One;12(9):e0184681, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Phytolacca americana L. (pokeweed) has metal phytoremediation potential, but little is known about its metal accumulation-related genes. In this study, the de novo sequencing of total RNA produced 53.15 million reads covering 10.63 gigabases of transcriptome raw data in cadmium (Cd)-treated and untreated pokeweed. Of the 97,502 assembled unigenes, 42,197 had significant matches in a public database and were annotated accordingly. An expression level comparison between the samples revealed 1515 differentially expressed genes (DEGs), 923 down- and 592 up-regulated under Cd treatment. A KEGG pathway enrichment analysis of DEGs revealed that they were involved in 72 metabolism pathways, with photosynthesis, phenylalanine metabolism, ribosome, phenylpropanoid biosynthesis, flavonoid biosynthesis and carbon fixation in photosynthetic organisms containing 24, 18, 72, 14, 7 and 15 genes, respectively. Genes related to heavy metal tolerance, absorption, transport and accumulation were also identified, including 11 expansins, 8 nicotianamine synthases, 6 aquaporins, 4 ZRT/IRT-like proteins, 3 ABC transporters and 3 metallothioneins. The gene expression results of 12 randomly selected DEGs were validated using quantitative real-time PCR, and showed different response patterns to Cd in their roots, stems and leaves. These results may be helpful in increasing our understanding of heavy metal hyperaccumulators and in future phytoremediation applications. [Mh] Termos MeSH primário: Cádmio/toxicidade Phytolacca americana/genética Estresse Fisiológico Transcriptoma [Mh] Termos MeSH secundário: Phytolacca americana/efeitos dos fármacos Phytolacca americana/metabolismo Proteínas de Plantas/genética Proteínas de Plantas/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Proteins); 00BH33GNGH (Cadmium) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171012 [Lr] Data última revisão: 171012 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170913 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0184681

2 / 92

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28864671 [Au] Autor: Kutky M; Hudak KA [Ad] Endereço: Department of Biology, York University, Toronto, Ontario, Canada. [Ti] Título: Expression of an RNA glycosidase inhibits HIV-1 transactivation of transcription. [So] Source: Biochem J;474(20):3471-3483, 2017 Oct 05. [Is] ISSN: 1470-8728 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: HIV-1 (human immunodeficiency virus) transcription is primarily controlled by the virally encoded Tat (transactivator of transcription) protein and its interaction with the viral TAR (transcription response element) RNA element. Specifically, binding of a Tat-containing complex to TAR recruits cellular factors that promote elongation of the host RNA polymerase engaging the viral DNA template. Disruption of this interaction halts viral RNA transcription. In the present study, we investigated the effect of pokeweed antiviral protein (PAP), an RNA glycosidase (EC#: 3.2.2.22) synthesized by the pokeweed plant ( ), on transcription of HIV-1 mRNA. We show that co-expression of PAP with a proviral clone in culture cells resulted in a Tat-dependent decrease in viral mRNA levels. PAP reduced HIV-1 transcriptional activity by inhibiting Tat protein synthesis. The effects of PAP expression on host factors AP-1 (activator protein 1), NF-κB (nuclear factor kappa-light-chain-enhancer of activated B-cells) and specificity protein 1, which modulate HIV-1 transcription by binding to the viral LTR (5'-long terminal repeat), were also investigated. Only AP-1 showed a modest JNK pathway-dependent increase in activity in the presence of PAP; however, this activation was not sufficient to significantly enhance transcription from a partial viral LTR containing AP-1 binding sites. Therefore, the primary effect of PAP on HIV-1 transcription is to reduce viral RNA synthesis by decreasing the abundance of Tat. These findings provide a mechanistic explanation for the observed decrease in viral RNAs in cells expressing PAP and contribute to our understanding of the antiviral effects of this plant protein. [Mh] Termos MeSH primário: Regulação Enzimológica da Expressão Gênica HIV-1/metabolismo RNA Viral/biossíntese Proteínas Inativadoras de Ribossomos/biossíntese Transcrição Genética/fisiologia Ativação Transcricional/fisiologia [Mh] Termos MeSH secundário: Sobrevivência Celular/fisiologia Células HEK293 HIV-1/genética Seres Humanos Células Jurkat Phytolacca americana RNA Viral/genética Proteínas Inativadoras de Ribossomos/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (RNA, Viral); EC 3.2.2.22 (Ribosome Inactivating Proteins) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171012 [Lr] Data última revisão: 171012 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170903 [St] Status: MEDLINE [do] DOI: 10.1042/BCJ20170353

3 / 92

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 27265341 [Au] Autor: Li N; Yang W; Fang S; Li X; Liu Z; Leng X; An S [Ad] Endereço: School of Life Science, Nanjing University, Nanjing, China. [Ti] Título: Dispersal of invasive Phytolacca americana seeds by birds in an urban garden in China. [So] Source: Integr Zool;12(1):26-31, 2017 Jan. [Is] ISSN: 1749-4877 [Cp] País de publicação: Australia [La] Idioma: eng [Ab] Resumo: Although seed dispersal is a key process determining the regeneration and spread of invasive plant populations, few studies have explicitly addressed the link between dispersal vector behavior and seedling recruitment to gain insight into the invasion process within an urban garden context. We evaluated the role of bird vectors in the dispersal of pokeweed (Phytolacca americana), a North American herb that is invasive in urban gardens in China. Fruiting P. americana attracted both generalist and specialist bird species that fed on and dispersed its seeds. The generalist species Pycnonotus sinensis and Urocissa erythrorhyncha were the most frequent dispersers. Seedling numbers of P. americana were strongly associated with the perching behavior of frugivorous birds. If newly recruited bird species use seedling-safe perching sites, the P. americana will regenerate faster, which would enhance its invasive potential. Based on our observations, we conclude that the 2 main bird vectors, P. sinensis and U. erythrorhyncha, provide potential effective dispersal agents for P. americana. Our results highlight the role of native birds in seed dispersal of invasive plants in urban gardens. [Mh] Termos MeSH primário: Aves/fisiologia Comportamento Alimentar Phytolacca americana Dispersão de Sementes [Mh] Termos MeSH secundário: Animais China Cidades Frutas Jardins Espécies Introduzidas [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170203 [Lr] Data última revisão: 170203 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160607 [St] Status: MEDLINE [do] DOI: 10.1111/1749-4877.12214

4 / 92

MEDLINE

seleciona para imprimir Fotocópia

[PMID]: 27032200 [Au] Autor: Shimoda K; Kubota N; Uesugi D; Tanigawa M; Hamada H [Ti] Título: Hydroxylation and Glycosylation of Phenylpropanoids by Cultured Cells of Phytolacca americana. [So] Source: Nat Prod Commun;11(2):197-8, 2016 Feb. [Is] ISSN: 1934-578X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Hydroxylation and glycosylation of cinnamic acid, p-coumaric acid, caffeic acid, and ferulic acid were investigated using cultured plant cells of Phytolacca americana as biocatalysts. Regioselective hydroxylation at the 4-position of cinnamic acid and 3-position of p-coumaric acid was observed. Although cinnamic acid was transformed to mono-glucoside products, di-glycosylation occurred in the case of the biotransformation of p-coumaric acid, caffeic acid, and ferulic acid. [Mh] Termos MeSH primário: Cinamatos/química Ácidos Cumáricos/química Phytolacca americana/citologia Phytolacca americana/metabolismo [Mh] Termos MeSH secundário: Biotransformação Células Cultivadas Glicosilação Hidroxilação Estrutura Molecular Propionatos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cinnamates); 0 (Coumaric Acids); 0 (Propionates); AVM951ZWST (ferulic acid); IBS9D1EU3J (trans-3-(4'-hydroxyphenyl)-2-propenoic acid); U14A832J8D (cinnamic acid) [Em] Mês de entrada: 1605 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160402 [St] Status: MEDLINE

5 / 92

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26927807 [Au] Autor: Kobayashi Y; Sato H; Yorita M; Nakayama H; Miyazato H; Sugimoto K; Jippo T [Ad] Endereço: a Faculty of Human Life Sciences, Department of Food and Nutrition , Senri Kinran University , Suita , Japan. [Ti] Título: Inhibitory effects of geranium essential oil and its major component, citronellol, on degranulation and cytokine production by mast cells. [So] Source: Biosci Biotechnol Biochem;80(6):1172-8, 2016 Jun. [Is] ISSN: 1347-6947 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: We investigated the effects of geranium essential oil (GEO) on anaphylaxis. GEO can exert antioxidant and anti-inflammatory effects, but its roles in allergic reactions are incompletely understood. Here, we used mouse cells to show that GEO inhibited the degranulation of cultured mast cells (CMCs). Citronellol is the major component of GEO and inhibited CMC degranulation. The l-enantiomer of citronellol more effectively suppressed CMC degranulation than did d-citronellol. We also examined whether citronellol could inhibit the immunoglobulin (Ig) E-induced production of tumor necrosis factor (TNF)-α. Treatment with various concentrations of citronellol before CMC activation with IgE significantly inhibited the induction of TNF-α in a dose-dependent manner. Mechanistically, citronellol suppressed the phosphorylation of mitogen-activated protein kinase (ERK), which is critical for ERK activation and the production of inflammatory cytokines in mast cells. These findings suggest that citronellol may represent a candidate compound for the effective treatment of allergic diseases. [Mh] Termos MeSH primário: Degranulação Celular/efeitos dos fármacos Geranium/química Mastócitos/efeitos dos fármacos Monoterpenos/farmacologia Óleos Voláteis/farmacologia [Mh] Termos MeSH secundário: Animais Citocinas/antagonistas & inibidores Citocinas/genética Citocinas/imunologia Relação Dose-Resposta a Droga MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores MAP Quinases Reguladas por Sinal Extracelular/genética MAP Quinases Reguladas por Sinal Extracelular/imunologia Cromatografia Gasosa-Espectrometria de Massas Regulação da Expressão Gênica Imunoglobulina E/farmacologia Masculino Mastócitos/citologia Mastócitos/imunologia Camundongos Camundongos Endogâmicos ICR Fosforilação Phytolacca americana/química Extratos Vegetais/química Extratos Vegetais/farmacologia Estereoisomerismo Fator de Necrose Tumoral alfa/genética Fator de Necrose Tumoral alfa/imunologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cytokines); 0 (Monoterpenes); 0 (Oils, Volatile); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha); 37341-29-0 (Immunoglobulin E); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); P01OUT964K (citronellol) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170117 [Lr] Data última revisão: 170117 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160302 [St] Status: MEDLINE [do] DOI: 10.1080/09168451.2016.1148573

6 / 92

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26449874 [Au] Autor: Klenov A; Neller KC; Burns LA; Krivdova G; Hudak KA [Ad] Endereço: Department of Biology, York University, Toronto, Ontario, Canada. [Ti] Título: A small RNA targets pokeweed antiviral protein transcript. [So] Source: Physiol Plant;156(3):241-51, 2016 Mar. [Is] ISSN: 1399-3054 [Cp] País de publicação: Denmark [La] Idioma: eng [Ab] Resumo: Ribosome-inactivating proteins (RIPs) are a class of plant defense proteins with N-glycosidase activity (EC 3.2.2.22). Pokeweed antiviral protein (PAP) is a Type I RIP isolated from the pokeweed plant, Phytolacca americana, thought to confer broad-spectrum virus resistance in this plant. Through a combination of standard molecular techniques and RNA sequencing analysis, we report here that a small RNA binds and cleaves the open reading frame of PAP mRNA. Additionally, sRNA targeting of PAP is dependent on jasmonic acid (JA), a plant hormone important for defense against pathogen infection and herbivory. Levels of small RNA increased with JA treatment, as did levels of PAP mRNA and protein, suggesting that the small RNA functions to moderate the expression of PAP in response to this hormone. The association between JA and PAP expression, mediated by sRNA299, situates PAP within a signaling pathway initiated by biotic stress. The consensus sequence of sRNA299 was obtained through bioinformatic analysis of pokeweed small RNA sequencing. To our knowledge, this is the first account of a sRNA targeting a RIP gene. [Mh] Termos MeSH primário: RNA de Plantas/metabolismo Proteínas Inativadoras de Ribossomos Tipo 1/metabolismo [Mh] Termos MeSH secundário: Sequência de Bases Ciclopentanos/farmacologia Regulação da Expressão Gênica de Plantas/efeitos dos fármacos Dados de Sequência Molecular Nucleotídeos/genética Oxilipinas/farmacologia Phytolacca americana/efeitos dos fármacos Phytolacca americana/genética Proteínas de Plantas/genética Proteínas de Plantas/metabolismo RNA Mensageiro/genética RNA Mensageiro/metabolismo Reprodutibilidade dos Testes Proteínas Inativadoras de Ribossomos Tipo 1/genética Análise de Sequência de RNA Sítio de Iniciação de Transcrição [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Cyclopentanes); 0 (Nucleotides); 0 (Oxylipins); 0 (Plant Proteins); 0 (RNA, Messenger); 0 (RNA, Plant); 0 (Ribosome Inactivating Proteins, Type 1); 6RI5N05OWW (jasmonic acid); EC 3.2.2.22 (pokeweed antiviral protein) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151010 [St] Status: MEDLINE [do] DOI: 10.1111/ppl.12393

7 / 92

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26097089 [Au] Autor: Huang W; Ding J [Ad] Endereço: Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China. [Ti] Título: Effects of generalist herbivory on resistance and resource allocation by the invasive plant, Phytolacca americana. [So] Source: Insect Sci;23(2):191-9, 2016 Apr. [Is] ISSN: 1744-7917 [Cp] País de publicação: Australia [La] Idioma: eng [Ab] Resumo: Successful invasions by exotic plants are often attributed to a loss of co-evolved specialists and a re-allocation of resources from defense to growth and reproduction. However, invasive plants are rarely completely released from insect herbivory because they are frequently attacked by generalists in their introduced ranges. The novel generalist community may also affect the invasive plant's defensive strategies and resource allocation. Here, we tested this hypothesis using American pokeweed (Phytolacca americana L.), a species that has become invasive in China, which is native to North America. We examined resistance, tolerance, growth and reproduction of plant populations from both China and the USA when plants were exposed to natural generalist herbivores in China. We found that leaf damage was greater for invasive populations than for native populations, indicating that plants from invasive ranges had lower resistance to herbivory than those from native ranges. A regression of the percentage of leaf damage against mass showed that there was no significant difference in tolerance between invasive and native populations, even though the shoot, root, fruit and total mass were larger for invasive populations than for native populations. These results suggest that generalist herbivores are important drivers mediating the defensive strategies and resource allocation of the invasive American pokeweed. [Mh] Termos MeSH primário: Herbivoria Insetos/fisiologia Phytolacca americana/fisiologia [Mh] Termos MeSH secundário: Animais China Espécies Introduzidas Phytolacca americana/crescimento & desenvolvimento Folhas de Planta/crescimento & desenvolvimento Folhas de Planta/fisiologia Estados Unidos [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1612 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150623 [St] Status: MEDLINE [do] DOI: 10.1111/1744-7917.12244

8 / 92

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 25894210 [Au] Autor: Yu H; Gong L; Wang X; Wu H; Zhao T; Wang K; Cui X; Chen L [Ad] Endereço: a School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , PR China . [Ti] Título: Rabbit conjunctivae edema and release of NO, TNF-α, and IL-1ß from macrophages induced by fractions and esculentosides isolated from Phytolacca americana. [So] Source: Pharm Biol;54(1):98-104, 2016. [Is] ISSN: 1744-5116 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: CONTEXT: The roots of Phytolacca americana L. (Phytolaccaceae) may be toxic. Despite heated controversy over the toxic compounds of P. americana, especially esculentosides, relevant studies remain scarce. OBJECTIVE: The objective of this study is to screen the toxic fractions and compounds of P. americana, to determine the controlling indices, and to provide evidence for unraveling the mechanism. MATERIALS AND METHODS: Petroleum ether (PE), CH2Cl2, n-BuOH, and water fractions were isolated from 70% ethanol extract of P. americana. The n-BuOH fraction was dissolved in 50% ethanol and precipitated by adding ethyl ether. The resultant supernatants and precipitates were referred to as SUPs and SEDs fractions, respectively. SUPs fraction was separated by column chromatography into four main stimulating esculentosides that were identified by HR-ESI/MS and NMR as EsA, EsB, EsC, and EsF. The irritating effects of esculentosides on rabbit conjunctivae (500 µg/eye) was observed by pathological examination and those on macrophages (5, 25, 50 and 100 µg/mL) were evaluated by detecting changes of NO, TNF-α, and IL-1ß levels. RESULTS AND DISCUSSION: n-BuOH, SUP fractions, and EsC induced severe conjunctival edema. The four esculentosides induced dose-dependent releases of proinflammatory mediators NO, TNF-α, and IL-1ß from macrophages, and releasing amounts peaked after 2 h of treatment. EsC and EsF induced macrophages to release mediators most significantly. EsC (50 µg/mL) functioned more effectively than EsF did, and similarly n-BuOH and SUPs fractions functioned more effectively than the esculentoside mixture. Thus, the four esculentosides exerted proinflammatory effects synergistically. CONCLUSION: All extracted esculentosides, especially EsC, induced inflammatory stimulation. Phytolacca americana-induced irritation of the gastrointestinal tract may be associated with esculentosides such as EsC. [Mh] Termos MeSH primário: Túnica Conjuntiva/efeitos dos fármacos Conjuntivite/induzido quimicamente Edema/induzido quimicamente Mediadores da Inflamação/metabolismo Interleucina-1beta/metabolismo Macrófagos/efeitos dos fármacos Óxido Nítrico/metabolismo Phytolacca americana/toxicidade Extratos Vegetais/toxicidade Saponinas/toxicidade Fator de Necrose Tumoral alfa/metabolismo [Mh] Termos MeSH secundário: Animais Túnica Conjuntiva/imunologia Túnica Conjuntiva/metabolismo Conjuntivite/imunologia Conjuntivite/metabolismo Relação Dose-Resposta a Droga Edema/imunologia Edema/metabolismo Macrófagos/imunologia Macrófagos/metabolismo Masculino Camundongos Endogâmicos ICR Phytolacca americana/química Fitoterapia Extratos Vegetais/química Extratos Vegetais/isolamento & purificação Raízes de Plantas Plantas Medicinais Coelhos Medição de Risco Saponinas/química Saponinas/isolamento & purificação Solventes/química [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Inflammation Mediators); 0 (Interleukin-1beta); 0 (Plant Extracts); 0 (Saponins); 0 (Solvents); 0 (Tumor Necrosis Factor-alpha); 31C4KY9ESH (Nitric Oxide) [Em] Mês de entrada: 1609 [Cu] Atualização por classe: 151208 [Lr] Data última revisão: 151208 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150421 [St] Status: MEDLINE [do] DOI: 10.3109/13880209.2015.1016182

9 / 92

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26595875 [Au] Autor: Jung C; Hong JY; Bae SY; Kang SS; Park HJ; Lee SK [Ad] Endereço: College of Pharmacy, Natural Products Research Institute, Seoul National University , Seoul 151-742, Korea. [Ti] Título: Antitumor Activity of Americanin A Isolated from the Seeds of Phytolacca americana by Regulating the ATM/ATR Signaling Pathway and the Skp2-p27 Axis in Human Colon Cancer Cells. [So] Source: J Nat Prod;78(12):2983-93, 2015 Dec 24. [Is] ISSN: 1520-6025 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The antiproliferative and antitumor activities of americanin A (1), a neolignan isolated from the seeds of Phytolacca americana, were investigated in human colon cancer cells. Compound 1 inhibited the proliferation of HCT116 human colon cancer cells both in vitro and in vivo. The induction of G2/M cell-cycle arrest by 1 was concomitant with regulation of the ataxia telangiectasia-mutated/ATM and Rad3-related (ATM/ATR) signaling pathway. Treatment with 1 activated ATM and ATR, initiating the subsequent signal transduction cascades that include checkpoint kinase 1 (Chk1), checkpoint kinase 2 (Chk2), and tumor suppressor p53. Another line of evidence underlined the significance of 1 in regulation of the S phase kinase-associated protein 2 (Skp2)-p27 axis. Compound 1 targeted selectively Skp2 for degradation and thereby stabilized p27. Therefore, compound 1 suppressed the activity of cyclin B1 and its partner cell division cycle 2 (cdc2) to prevent entry into mitosis. Furthermore, prolonged treatment with 1 induced apoptosis by producing excessive reactive oxygen species. The intraperitoneal administration of 1 inhibited the growth of HCT116 tumor xenografts in nude mice without any overt toxicity. Modulation of the ATM/ATR signaling pathway and the Skp2-p27 axis might be plausible mechanisms of action for the antiproliferative and antitumor activities of 1 in human colon cancer cells. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação Antineoplásicos Fitogênicos/farmacologia Dioxinas/isolamento & purificação Dioxinas/farmacologia Phytolacca americana/química [Mh] Termos MeSH secundário: Animais Antineoplásicos Fitogênicos/química Apoptose Proteína Quinase CDC2/metabolismo Quinase do Ponto de Checagem 1 Quinase do Ponto de Checagem 2/metabolismo Neoplasias do Colo Dioxinas/química Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos Células HCT116 Seres Humanos Camundongos Camundongos Nus Estrutura Molecular Proteínas Quinases/metabolismo Sementes/química Transdução de Sinais Proteína Supressora de Tumor p53/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Dioxins); 0 (Tumor Suppressor Protein p53); 0 (americanin A); EC 2.7.- (Protein Kinases); EC 2.7.1.11 (Checkpoint Kinase 2); EC 2.7.11.1 (CHEK1 protein, human); EC 2.7.11.1 (Checkpoint Kinase 1); EC 2.7.11.1 (Chek1 protein, mouse); EC 2.7.11.22 (CDC2 Protein Kinase) [Em] Mês de entrada: 1605 [Cu] Atualização por classe: 161126 [Lr] Data última revisão: 161126 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151124 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jnatprod.5b00743

10 / 92

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26250502 [Au] Autor: Shimoda K; Kubota N; Uesugi D; Hamada H; Tanigawa M; Hamada H [Ad] Endereço: Department of Chemistry, Faculty of Medicine, Oita University, Oita, Japan. [Ti] Título: Synthesis and pharmacological evaluation of glycosides of resveratrol, pterostilbene, and piceatannol. [So] Source: Ann N Y Acad Sci;1348(1):141-9, 2015 Aug. [Is] ISSN: 1749-6632 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: To enhance their water solubility and pharmacological activities, the stilbenes resveratrol, pterostilbene, and piceatannol were glycosylated to their monoglucosides (ß-glucosides) and diglycosides (ß-maltosides) by cultured cells and cyclodextrin glucanotransferase (CGTase). Cultured cells of Phytolacca americana and glucosyltransferase (PaGT) were capable of glucosylation of resveratrol to its 3- and 4'-ß-glucosides. Pterostilbene was slightly transformed into its 4'-ß-glucoside by P. americana cells. Piceatannol was readily converted into piceatannol 4'-ß-glucoside, with the highest yield among the three substrates. The 3- and 4'-ß-glucosides of resveratrol were subjected to further glycosylation by CGTase to give 3- and 4'-ß-maltoside derivatives. The inhibitory action of resveratrol and pterostilbene toward histamine release induced with compound 48/80 from rat peritoneal mast cells was improved by ß-glucosylation and/or ß-maltosylation (i.e., the inhibitory activity for histamine release of the 3- and 4'-ß-glucosides of resveratrol, the 3- and 4'-ß-maltosides of resveratrol, and the 4'-ß-glucoside of pterostilbene was higher than that of the corresponding aglycones, resveratrol and pterostilbene, respectively). In addition, the phosphodiesterase (PDE) inhibitory activity of resveratrol and pterostilbene was enhanced by ß-glucosylation and/or ß-maltosylation (i.e., the PDE inhibitory activities of the 3- and 4'-ß-glucosides of resveratrol, the 4'-ß-maltoside of resveratrol, and the 4'-ß-glucoside of pterostilbene were higher than those of the corresponding aglycones, resveratrol and pterostilbene, respectively). [Mh] Termos MeSH primário: Glicosídeos/farmacologia Estilbenos/farmacologia [Mh] Termos MeSH secundário: Animais Antialérgicos/química Antialérgicos/metabolismo Antialérgicos/farmacologia Células Cultivadas Avaliação Pré-Clínica de Medicamentos Depuradores de Radicais Livres/química Depuradores de Radicais Livres/metabolismo Depuradores de Radicais Livres/farmacologia Glicosídeos/biossíntese Glicosídeos/química Glicosilação Concentração Inibidora 50 Inibidores de Fosfodiesterase/química Inibidores de Fosfodiesterase/metabolismo Inibidores de Fosfodiesterase/farmacologia Phytolacca americana/citologia Phytolacca americana/metabolismo Extratos Vegetais/biossíntese Extratos Vegetais/química Extratos Vegetais/farmacologia Ratos Solubilidade Estilbenos/química Estilbenos/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Allergic Agents); 0 (Free Radical Scavengers); 0 (Glycosides); 0 (Phosphodiesterase Inhibitors); 0 (Plant Extracts); 0 (Stilbenes); 26R60S6A5I (pterostilbene); 6KS3LS0D4F (3,3',4,5'-tetrahydroxystilbene); Q369O8926L (resveratrol) [Em] Mês de entrada: 1512 [Cu] Atualização por classe: 161125 [Lr] Data última revisão: 161125 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150808 [St] Status: MEDLINE [do] DOI: 10.1111/nyas.12836

---

página 1 de 10

ir para página

Refinar a pesquisa

Base de dados : MEDLINE

Formulário avançado

		Pesquisar	no campo
1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde