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  1 / 92 MEDLINE  
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[PMID]:28898278
[Au] Autor:Chen Y; Zhi J; Zhang H; Li J; Zhao Q; Xu J
[Ad] Endereço:National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.
[Ti] Título:Transcriptome analysis of Phytolacca americana L. in response to cadmium stress.
[So] Source:PLoS One;12(9):e0184681, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phytolacca americana L. (pokeweed) has metal phytoremediation potential, but little is known about its metal accumulation-related genes. In this study, the de novo sequencing of total RNA produced 53.15 million reads covering 10.63 gigabases of transcriptome raw data in cadmium (Cd)-treated and untreated pokeweed. Of the 97,502 assembled unigenes, 42,197 had significant matches in a public database and were annotated accordingly. An expression level comparison between the samples revealed 1515 differentially expressed genes (DEGs), 923 down- and 592 up-regulated under Cd treatment. A KEGG pathway enrichment analysis of DEGs revealed that they were involved in 72 metabolism pathways, with photosynthesis, phenylalanine metabolism, ribosome, phenylpropanoid biosynthesis, flavonoid biosynthesis and carbon fixation in photosynthetic organisms containing 24, 18, 72, 14, 7 and 15 genes, respectively. Genes related to heavy metal tolerance, absorption, transport and accumulation were also identified, including 11 expansins, 8 nicotianamine synthases, 6 aquaporins, 4 ZRT/IRT-like proteins, 3 ABC transporters and 3 metallothioneins. The gene expression results of 12 randomly selected DEGs were validated using quantitative real-time PCR, and showed different response patterns to Cd in their roots, stems and leaves. These results may be helpful in increasing our understanding of heavy metal hyperaccumulators and in future phytoremediation applications.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Phytolacca americana/genética
Estresse Fisiológico
Transcriptoma
[Mh] Termos MeSH secundário: Phytolacca americana/efeitos dos fármacos
Phytolacca americana/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 00BH33GNGH (Cadmium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184681


  2 / 92 MEDLINE  
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[PMID]:28864671
[Au] Autor:Kutky M; Hudak KA
[Ad] Endereço:Department of Biology, York University, Toronto, Ontario, Canada.
[Ti] Título:Expression of an RNA glycosidase inhibits HIV-1 transactivation of transcription.
[So] Source:Biochem J;474(20):3471-3483, 2017 Oct 05.
[Is] ISSN:1470-8728
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:HIV-1 (human immunodeficiency virus) transcription is primarily controlled by the virally encoded Tat (transactivator of transcription) protein and its interaction with the viral TAR (transcription response element) RNA element. Specifically, binding of a Tat-containing complex to TAR recruits cellular factors that promote elongation of the host RNA polymerase engaging the viral DNA template. Disruption of this interaction halts viral RNA transcription. In the present study, we investigated the effect of pokeweed antiviral protein (PAP), an RNA glycosidase (EC#: 3.2.2.22) synthesized by the pokeweed plant ( ), on transcription of HIV-1 mRNA. We show that co-expression of PAP with a proviral clone in culture cells resulted in a Tat-dependent decrease in viral mRNA levels. PAP reduced HIV-1 transcriptional activity by inhibiting Tat protein synthesis. The effects of PAP expression on host factors AP-1 (activator protein 1), NF-κB (nuclear factor kappa-light-chain-enhancer of activated B-cells) and specificity protein 1, which modulate HIV-1 transcription by binding to the viral LTR (5'-long terminal repeat), were also investigated. Only AP-1 showed a modest JNK pathway-dependent increase in activity in the presence of PAP; however, this activation was not sufficient to significantly enhance transcription from a partial viral LTR containing AP-1 binding sites. Therefore, the primary effect of PAP on HIV-1 transcription is to reduce viral RNA synthesis by decreasing the abundance of Tat. These findings provide a mechanistic explanation for the observed decrease in viral RNAs in cells expressing PAP and contribute to our understanding of the antiviral effects of this plant protein.
[Mh] Termos MeSH primário: Regulação Enzimológica da Expressão Gênica
HIV-1/metabolismo
RNA Viral/biossíntese
Proteínas Inativadoras de Ribossomos/biossíntese
Transcrição Genética/fisiologia
Ativação Transcricional/fisiologia
[Mh] Termos MeSH secundário: Sobrevivência Celular/fisiologia
Células HEK293
HIV-1/genética
Seres Humanos
Células Jurkat
Phytolacca americana
RNA Viral/genética
Proteínas Inativadoras de Ribossomos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); EC 3.2.2.22 (Ribosome Inactivating Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE
[do] DOI:10.1042/BCJ20170353


  3 / 92 MEDLINE  
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[PMID]:27265341
[Au] Autor:Li N; Yang W; Fang S; Li X; Liu Z; Leng X; An S
[Ad] Endereço:School of Life Science, Nanjing University, Nanjing, China.
[Ti] Título:Dispersal of invasive Phytolacca americana seeds by birds in an urban garden in China.
[So] Source:Integr Zool;12(1):26-31, 2017 Jan.
[Is] ISSN:1749-4877
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Although seed dispersal is a key process determining the regeneration and spread of invasive plant populations, few studies have explicitly addressed the link between dispersal vector behavior and seedling recruitment to gain insight into the invasion process within an urban garden context. We evaluated the role of bird vectors in the dispersal of pokeweed (Phytolacca americana), a North American herb that is invasive in urban gardens in China. Fruiting P. americana attracted both generalist and specialist bird species that fed on and dispersed its seeds. The generalist species Pycnonotus sinensis and Urocissa erythrorhyncha were the most frequent dispersers. Seedling numbers of P. americana were strongly associated with the perching behavior of frugivorous birds. If newly recruited bird species use seedling-safe perching sites, the P. americana will regenerate faster, which would enhance its invasive potential. Based on our observations, we conclude that the 2 main bird vectors, P. sinensis and U. erythrorhyncha, provide potential effective dispersal agents for P. americana. Our results highlight the role of native birds in seed dispersal of invasive plants in urban gardens.
[Mh] Termos MeSH primário: Aves/fisiologia
Comportamento Alimentar
Phytolacca americana
Dispersão de Sementes
[Mh] Termos MeSH secundário: Animais
China
Cidades
Frutas
Jardins
Espécies Introduzidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170203
[Lr] Data última revisão:
170203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160607
[St] Status:MEDLINE
[do] DOI:10.1111/1749-4877.12214


  4 / 92 MEDLINE  
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[PMID]:27032200
[Au] Autor:Shimoda K; Kubota N; Uesugi D; Tanigawa M; Hamada H
[Ti] Título:Hydroxylation and Glycosylation of Phenylpropanoids by Cultured Cells of Phytolacca americana.
[So] Source:Nat Prod Commun;11(2):197-8, 2016 Feb.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hydroxylation and glycosylation of cinnamic acid, p-coumaric acid, caffeic acid, and ferulic acid were investigated using cultured plant cells of Phytolacca americana as biocatalysts. Regioselective hydroxylation at the 4-position of cinnamic acid and 3-position of p-coumaric acid was observed. Although cinnamic acid was transformed to mono-glucoside products, di-glycosylation occurred in the case of the biotransformation of p-coumaric acid, caffeic acid, and ferulic acid.
[Mh] Termos MeSH primário: Cinamatos/química
Ácidos Cumáricos/química
Phytolacca americana/citologia
Phytolacca americana/metabolismo
[Mh] Termos MeSH secundário: Biotransformação
Células Cultivadas
Glicosilação
Hidroxilação
Estrutura Molecular
Propionatos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cinnamates); 0 (Coumaric Acids); 0 (Propionates); AVM951ZWST (ferulic acid); IBS9D1EU3J (trans-3-(4'-hydroxyphenyl)-2-propenoic acid); U14A832J8D (cinnamic acid)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE


  5 / 92 MEDLINE  
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[PMID]:26927807
[Au] Autor:Kobayashi Y; Sato H; Yorita M; Nakayama H; Miyazato H; Sugimoto K; Jippo T
[Ad] Endereço:a Faculty of Human Life Sciences, Department of Food and Nutrition , Senri Kinran University , Suita , Japan.
[Ti] Título:Inhibitory effects of geranium essential oil and its major component, citronellol, on degranulation and cytokine production by mast cells.
[So] Source:Biosci Biotechnol Biochem;80(6):1172-8, 2016 Jun.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We investigated the effects of geranium essential oil (GEO) on anaphylaxis. GEO can exert antioxidant and anti-inflammatory effects, but its roles in allergic reactions are incompletely understood. Here, we used mouse cells to show that GEO inhibited the degranulation of cultured mast cells (CMCs). Citronellol is the major component of GEO and inhibited CMC degranulation. The l-enantiomer of citronellol more effectively suppressed CMC degranulation than did d-citronellol. We also examined whether citronellol could inhibit the immunoglobulin (Ig) E-induced production of tumor necrosis factor (TNF)-α. Treatment with various concentrations of citronellol before CMC activation with IgE significantly inhibited the induction of TNF-α in a dose-dependent manner. Mechanistically, citronellol suppressed the phosphorylation of mitogen-activated protein kinase (ERK), which is critical for ERK activation and the production of inflammatory cytokines in mast cells. These findings suggest that citronellol may represent a candidate compound for the effective treatment of allergic diseases.
[Mh] Termos MeSH primário: Degranulação Celular/efeitos dos fármacos
Geranium/química
Mastócitos/efeitos dos fármacos
Monoterpenos/farmacologia
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Animais
Citocinas/antagonistas & inibidores
Citocinas/genética
Citocinas/imunologia
Relação Dose-Resposta a Droga
MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores
MAP Quinases Reguladas por Sinal Extracelular/genética
MAP Quinases Reguladas por Sinal Extracelular/imunologia
Cromatografia Gasosa-Espectrometria de Massas
Regulação da Expressão Gênica
Imunoglobulina E/farmacologia
Masculino
Mastócitos/citologia
Mastócitos/imunologia
Camundongos
Camundongos Endogâmicos ICR
Fosforilação
Phytolacca americana/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Estereoisomerismo
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Monoterpenes); 0 (Oils, Volatile); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha); 37341-29-0 (Immunoglobulin E); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); P01OUT964K (citronellol)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2016.1148573


  6 / 92 MEDLINE  
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[PMID]:26449874
[Au] Autor:Klenov A; Neller KC; Burns LA; Krivdova G; Hudak KA
[Ad] Endereço:Department of Biology, York University, Toronto, Ontario, Canada.
[Ti] Título:A small RNA targets pokeweed antiviral protein transcript.
[So] Source:Physiol Plant;156(3):241-51, 2016 Mar.
[Is] ISSN:1399-3054
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Ribosome-inactivating proteins (RIPs) are a class of plant defense proteins with N-glycosidase activity (EC 3.2.2.22). Pokeweed antiviral protein (PAP) is a Type I RIP isolated from the pokeweed plant, Phytolacca americana, thought to confer broad-spectrum virus resistance in this plant. Through a combination of standard molecular techniques and RNA sequencing analysis, we report here that a small RNA binds and cleaves the open reading frame of PAP mRNA. Additionally, sRNA targeting of PAP is dependent on jasmonic acid (JA), a plant hormone important for defense against pathogen infection and herbivory. Levels of small RNA increased with JA treatment, as did levels of PAP mRNA and protein, suggesting that the small RNA functions to moderate the expression of PAP in response to this hormone. The association between JA and PAP expression, mediated by sRNA299, situates PAP within a signaling pathway initiated by biotic stress. The consensus sequence of sRNA299 was obtained through bioinformatic analysis of pokeweed small RNA sequencing. To our knowledge, this is the first account of a sRNA targeting a RIP gene.
[Mh] Termos MeSH primário: RNA de Plantas/metabolismo
Proteínas Inativadoras de Ribossomos Tipo 1/metabolismo
[Mh] Termos MeSH secundário: Sequência de Bases
Ciclopentanos/farmacologia
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Dados de Sequência Molecular
Nucleotídeos/genética
Oxilipinas/farmacologia
Phytolacca americana/efeitos dos fármacos
Phytolacca americana/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Reprodutibilidade dos Testes
Proteínas Inativadoras de Ribossomos Tipo 1/genética
Análise de Sequência de RNA
Sítio de Iniciação de Transcrição
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Nucleotides); 0 (Oxylipins); 0 (Plant Proteins); 0 (RNA, Messenger); 0 (RNA, Plant); 0 (Ribosome Inactivating Proteins, Type 1); 6RI5N05OWW (jasmonic acid); EC 3.2.2.22 (pokeweed antiviral protein)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151010
[St] Status:MEDLINE
[do] DOI:10.1111/ppl.12393


  7 / 92 MEDLINE  
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[PMID]:26097089
[Au] Autor:Huang W; Ding J
[Ad] Endereço:Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
[Ti] Título:Effects of generalist herbivory on resistance and resource allocation by the invasive plant, Phytolacca americana.
[So] Source:Insect Sci;23(2):191-9, 2016 Apr.
[Is] ISSN:1744-7917
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Successful invasions by exotic plants are often attributed to a loss of co-evolved specialists and a re-allocation of resources from defense to growth and reproduction. However, invasive plants are rarely completely released from insect herbivory because they are frequently attacked by generalists in their introduced ranges. The novel generalist community may also affect the invasive plant's defensive strategies and resource allocation. Here, we tested this hypothesis using American pokeweed (Phytolacca americana L.), a species that has become invasive in China, which is native to North America. We examined resistance, tolerance, growth and reproduction of plant populations from both China and the USA when plants were exposed to natural generalist herbivores in China. We found that leaf damage was greater for invasive populations than for native populations, indicating that plants from invasive ranges had lower resistance to herbivory than those from native ranges. A regression of the percentage of leaf damage against mass showed that there was no significant difference in tolerance between invasive and native populations, even though the shoot, root, fruit and total mass were larger for invasive populations than for native populations. These results suggest that generalist herbivores are important drivers mediating the defensive strategies and resource allocation of the invasive American pokeweed.
[Mh] Termos MeSH primário: Herbivoria
Insetos/fisiologia
Phytolacca americana/fisiologia
[Mh] Termos MeSH secundário: Animais
China
Espécies Introduzidas
Phytolacca americana/crescimento & desenvolvimento
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/fisiologia
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150623
[St] Status:MEDLINE
[do] DOI:10.1111/1744-7917.12244


  8 / 92 MEDLINE  
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[PMID]:25894210
[Au] Autor:Yu H; Gong L; Wang X; Wu H; Zhao T; Wang K; Cui X; Chen L
[Ad] Endereço:a School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , PR China .
[Ti] Título:Rabbit conjunctivae edema and release of NO, TNF-α, and IL-1ß from macrophages induced by fractions and esculentosides isolated from Phytolacca americana.
[So] Source:Pharm Biol;54(1):98-104, 2016.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: The roots of Phytolacca americana L. (Phytolaccaceae) may be toxic. Despite heated controversy over the toxic compounds of P. americana, especially esculentosides, relevant studies remain scarce. OBJECTIVE: The objective of this study is to screen the toxic fractions and compounds of P. americana, to determine the controlling indices, and to provide evidence for unraveling the mechanism. MATERIALS AND METHODS: Petroleum ether (PE), CH2Cl2, n-BuOH, and water fractions were isolated from 70% ethanol extract of P. americana. The n-BuOH fraction was dissolved in 50% ethanol and precipitated by adding ethyl ether. The resultant supernatants and precipitates were referred to as SUPs and SEDs fractions, respectively. SUPs fraction was separated by column chromatography into four main stimulating esculentosides that were identified by HR-ESI/MS and NMR as EsA, EsB, EsC, and EsF. The irritating effects of esculentosides on rabbit conjunctivae (500 µg/eye) was observed by pathological examination and those on macrophages (5, 25, 50 and 100 µg/mL) were evaluated by detecting changes of NO, TNF-α, and IL-1ß levels. RESULTS AND DISCUSSION: n-BuOH, SUP fractions, and EsC induced severe conjunctival edema. The four esculentosides induced dose-dependent releases of proinflammatory mediators NO, TNF-α, and IL-1ß from macrophages, and releasing amounts peaked after 2 h of treatment. EsC and EsF induced macrophages to release mediators most significantly. EsC (50 µg/mL) functioned more effectively than EsF did, and similarly n-BuOH and SUPs fractions functioned more effectively than the esculentoside mixture. Thus, the four esculentosides exerted proinflammatory effects synergistically. CONCLUSION: All extracted esculentosides, especially EsC, induced inflammatory stimulation. Phytolacca americana-induced irritation of the gastrointestinal tract may be associated with esculentosides such as EsC.
[Mh] Termos MeSH primário: Túnica Conjuntiva/efeitos dos fármacos
Conjuntivite/induzido quimicamente
Edema/induzido quimicamente
Mediadores da Inflamação/metabolismo
Interleucina-1beta/metabolismo
Macrófagos/efeitos dos fármacos
Óxido Nítrico/metabolismo
Phytolacca americana/toxicidade
Extratos Vegetais/toxicidade
Saponinas/toxicidade
Fator de Necrose Tumoral alfa/metabolismo
[Mh] Termos MeSH secundário: Animais
Túnica Conjuntiva/imunologia
Túnica Conjuntiva/metabolismo
Conjuntivite/imunologia
Conjuntivite/metabolismo
Relação Dose-Resposta a Droga
Edema/imunologia
Edema/metabolismo
Macrófagos/imunologia
Macrófagos/metabolismo
Masculino
Camundongos Endogâmicos ICR
Phytolacca americana/química
Fitoterapia
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Raízes de Plantas
Plantas Medicinais
Coelhos
Medição de Risco
Saponinas/química
Saponinas/isolamento & purificação
Solventes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Inflammation Mediators); 0 (Interleukin-1beta); 0 (Plant Extracts); 0 (Saponins); 0 (Solvents); 0 (Tumor Necrosis Factor-alpha); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151208
[Lr] Data última revisão:
151208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150421
[St] Status:MEDLINE
[do] DOI:10.3109/13880209.2015.1016182


  9 / 92 MEDLINE  
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[PMID]:26595875
[Au] Autor:Jung C; Hong JY; Bae SY; Kang SS; Park HJ; Lee SK
[Ad] Endereço:College of Pharmacy, Natural Products Research Institute, Seoul National University , Seoul 151-742, Korea.
[Ti] Título:Antitumor Activity of Americanin A Isolated from the Seeds of Phytolacca americana by Regulating the ATM/ATR Signaling Pathway and the Skp2-p27 Axis in Human Colon Cancer Cells.
[So] Source:J Nat Prod;78(12):2983-93, 2015 Dec 24.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The antiproliferative and antitumor activities of americanin A (1), a neolignan isolated from the seeds of Phytolacca americana, were investigated in human colon cancer cells. Compound 1 inhibited the proliferation of HCT116 human colon cancer cells both in vitro and in vivo. The induction of G2/M cell-cycle arrest by 1 was concomitant with regulation of the ataxia telangiectasia-mutated/ATM and Rad3-related (ATM/ATR) signaling pathway. Treatment with 1 activated ATM and ATR, initiating the subsequent signal transduction cascades that include checkpoint kinase 1 (Chk1), checkpoint kinase 2 (Chk2), and tumor suppressor p53. Another line of evidence underlined the significance of 1 in regulation of the S phase kinase-associated protein 2 (Skp2)-p27 axis. Compound 1 targeted selectively Skp2 for degradation and thereby stabilized p27. Therefore, compound 1 suppressed the activity of cyclin B1 and its partner cell division cycle 2 (cdc2) to prevent entry into mitosis. Furthermore, prolonged treatment with 1 induced apoptosis by producing excessive reactive oxygen species. The intraperitoneal administration of 1 inhibited the growth of HCT116 tumor xenografts in nude mice without any overt toxicity. Modulation of the ATM/ATR signaling pathway and the Skp2-p27 axis might be plausible mechanisms of action for the antiproliferative and antitumor activities of 1 in human colon cancer cells.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacologia
Dioxinas/isolamento & purificação
Dioxinas/farmacologia
Phytolacca americana/química
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/química
Apoptose
Proteína Quinase CDC2/metabolismo
Quinase do Ponto de Checagem 1
Quinase do Ponto de Checagem 2/metabolismo
Neoplasias do Colo
Dioxinas/química
Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos
Células HCT116
Seres Humanos
Camundongos
Camundongos Nus
Estrutura Molecular
Proteínas Quinases/metabolismo
Sementes/química
Transdução de Sinais
Proteína Supressora de Tumor p53/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Dioxins); 0 (Tumor Suppressor Protein p53); 0 (americanin A); EC 2.7.- (Protein Kinases); EC 2.7.1.11 (Checkpoint Kinase 2); EC 2.7.11.1 (CHEK1 protein, human); EC 2.7.11.1 (Checkpoint Kinase 1); EC 2.7.11.1 (Chek1 protein, mouse); EC 2.7.11.22 (CDC2 Protein Kinase)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151124
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.5b00743


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[PMID]:26250502
[Au] Autor:Shimoda K; Kubota N; Uesugi D; Hamada H; Tanigawa M; Hamada H
[Ad] Endereço:Department of Chemistry, Faculty of Medicine, Oita University, Oita, Japan.
[Ti] Título:Synthesis and pharmacological evaluation of glycosides of resveratrol, pterostilbene, and piceatannol.
[So] Source:Ann N Y Acad Sci;1348(1):141-9, 2015 Aug.
[Is] ISSN:1749-6632
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To enhance their water solubility and pharmacological activities, the stilbenes resveratrol, pterostilbene, and piceatannol were glycosylated to their monoglucosides (ß-glucosides) and diglycosides (ß-maltosides) by cultured cells and cyclodextrin glucanotransferase (CGTase). Cultured cells of Phytolacca americana and glucosyltransferase (PaGT) were capable of glucosylation of resveratrol to its 3- and 4'-ß-glucosides. Pterostilbene was slightly transformed into its 4'-ß-glucoside by P. americana cells. Piceatannol was readily converted into piceatannol 4'-ß-glucoside, with the highest yield among the three substrates. The 3- and 4'-ß-glucosides of resveratrol were subjected to further glycosylation by CGTase to give 3- and 4'-ß-maltoside derivatives. The inhibitory action of resveratrol and pterostilbene toward histamine release induced with compound 48/80 from rat peritoneal mast cells was improved by ß-glucosylation and/or ß-maltosylation (i.e., the inhibitory activity for histamine release of the 3- and 4'-ß-glucosides of resveratrol, the 3- and 4'-ß-maltosides of resveratrol, and the 4'-ß-glucoside of pterostilbene was higher than that of the corresponding aglycones, resveratrol and pterostilbene, respectively). In addition, the phosphodiesterase (PDE) inhibitory activity of resveratrol and pterostilbene was enhanced by ß-glucosylation and/or ß-maltosylation (i.e., the PDE inhibitory activities of the 3- and 4'-ß-glucosides of resveratrol, the 4'-ß-maltoside of resveratrol, and the 4'-ß-glucoside of pterostilbene were higher than those of the corresponding aglycones, resveratrol and pterostilbene, respectively).
[Mh] Termos MeSH primário: Glicosídeos/farmacologia
Estilbenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antialérgicos/química
Antialérgicos/metabolismo
Antialérgicos/farmacologia
Células Cultivadas
Avaliação Pré-Clínica de Medicamentos
Depuradores de Radicais Livres/química
Depuradores de Radicais Livres/metabolismo
Depuradores de Radicais Livres/farmacologia
Glicosídeos/biossíntese
Glicosídeos/química
Glicosilação
Concentração Inibidora 50
Inibidores de Fosfodiesterase/química
Inibidores de Fosfodiesterase/metabolismo
Inibidores de Fosfodiesterase/farmacologia
Phytolacca americana/citologia
Phytolacca americana/metabolismo
Extratos Vegetais/biossíntese
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Ratos
Solubilidade
Estilbenos/química
Estilbenos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Allergic Agents); 0 (Free Radical Scavengers); 0 (Glycosides); 0 (Phosphodiesterase Inhibitors); 0 (Plant Extracts); 0 (Stilbenes); 26R60S6A5I (pterostilbene); 6KS3LS0D4F (3,3',4,5'-tetrahydroxystilbene); Q369O8926L (resveratrol)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150808
[St] Status:MEDLINE
[do] DOI:10.1111/nyas.12836



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