Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.198.500.984.500 [Categoria DeCS] Referências encontradas : 161 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28714363 [Au] Autor: Jin H; Chen L; Wang S; Chao D [Ad] Endereço: 1 Department of Colorectal Surgery, The Second Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China. [Ti] Título: Portulaca oleracea extract can inhibit nodule formation of colon cancer stem cells by regulating gene expression of the Notch signal transduction pathway. [So] Source: Tumour Biol;39(7):1010428317708699, 2017 Jul. [Is] ISSN: 1423-0380 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: To investigate whether Portulaca oleracea extract affects tumor formation in colon cancer stem cells and its chemotherapy sensitivity. In addition, to analyze associated genetic changes within the Notch signal transduction pathway. Serum-free cultures of colon cancer cells (HT-29) and HT-29 cancer stem cells were treated with the chemotherapeutic drug 5-fluorouracil to assess sensitivity. Injections of the stem cells were also given to BALB/c mice to confirm tumor growth and note its characteristics. In addition, the effect of different concentrations of P. oleracea extract was tested on the growth of HT-29 colon cancer cells and HT-29 cancer stem cells, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. The effects of P. oleracea extract on the expression of ß-catenin, Notch1, and Notch2 in the HT-29 cells were studied using reverse transcription polymerase chain reaction and Western blotting. The tumor volume of the HT29 cells was two times larger than that of HT29 cancer stem cells. Treatment with P. oleracea extract inhibited the proliferation of both HT-29 cancer cells and HT-29 cancer stem cells at doses from 0.07 to 2.25 µg/mL. Apoptosis of HT-29 cancer cells and HT-29 cancer stem cells was assessed by flow cytometry; it was enhanced by the addition of P. oleracea extract. Finally, treatment with P. oleracea extract significantly downregulated the expression of the Notch1 and ß-catenin genes in both cell types. The results of this study show that P. oleracea extract inhibits the growth of colon cancer stem cells in a dose-dependent manner. Furthermore, it inhibits the expression of the Notch1 and ß-catenin genes. Taken together, this suggests that it may elicit its effects through regulatory and target genes that mediate the Notch signal transduction pathway. [Mh] Termos MeSH primário: Carcinogênese/efeitos dos fármacos Neoplasias do Colo/tratamento farmacológico Extratos Vegetais/administração & dosagem Receptor Notch1/genética beta Catenina/genética [Mh] Termos MeSH secundário: Animais Apoptose/efeitos dos fármacos Carcinogênese/genética Proliferação Celular/efeitos dos fármacos Neoplasias do Colo/genética Neoplasias do Colo/patologia Regulação Neoplásica da Expressão Gênica Células HT29 Seres Humanos Camundongos Células-Tronco Neoplásicas/efeitos dos fármacos Células-Tronco Neoplásicas/patologia Extratos Vegetais/química Portulaca/química Transdução de Sinais Ensaios Antitumorais Modelo de Xenoenxerto [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (NOTCH1 protein, human); 0 (Plant Extracts); 0 (Receptor, Notch1); 0 (beta Catenin) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170726 [Lr] Data última revisão: 170726 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170718 [St] Status: MEDLINE [do] DOI: 10.1177/1010428317708699

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[PMID]: 28106387 [Au] Autor: Li CY; Meng YH; Ying ZM; Xu N; Hao D; Gao MZ; Zhang WJ; Xu L; Gao YC; Stien D; Ying XX [Ad] Endereço: School of Pharmacy, Liaoning University of Traditional Chinese Medicine , 116600 Dalian, China. [Ti] Título: Correction to Three Novel Alkaloids from Portulaca oleracea L. and Their Anti-inflammatory Effects. [So] Source: J Agric Food Chem;65(4):993-994, 2017 Feb 01. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Oleracimine and oleracimine A were isolated from Portulaca oleracea L. and described in the J. Agric. Food Chem, but the alternative structures of the two compounds are proposed on the basis of NMR analyses. [Mh] Termos MeSH primário: Alcaloides/química Anti-Inflamatórios/química Extratos Vegetais/química Portulaca/química [Mh] Termos MeSH secundário: Alcaloides/farmacologia Anti-Inflamatórios/farmacologia Estrutura Molecular Extratos Vegetais/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Alkaloids); 0 (Anti-Inflammatory Agents); 0 (Plant Extracts) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170428 [Lr] Data última revisão: 170428 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170121 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.6b05659

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[PMID]: 28097483 [Au] Autor: Khandare RV; Desai SB; Bhujbal SS; Watharkar AD; Biradar SP; Pawar PK; Govindwar SP [Ad] Endereço: Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, 416004, India. krahulbt@gmail.com. [Ti] Título: Phytoremediation of fluoride with garden ornamentals Nerium oleander, Portulaca oleracea, and Pogonatherum crinitum. [So] Source: Environ Sci Pollut Res Int;24(7):6833-6839, 2017 Mar. [Is] ISSN: 1614-7499 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Nursery grown plants of Nerium oleander, Pogonatherum crinitum, and Portulaca oleracea were observed to remove fluoride up to 92, 80, and 73%, respectively, from NaF solution at the concentration of 10 mg L within 15 days. Concentration range of 10-50 mg L of fluoride revealed a constant decrease of removal from 92 to 51% within 15 days by N. oleander, while the biomass (one to five plants) showed enhancement in removal from 74 to 98% in 10 days. Translocation and bioaccumulation factors calculated after fluoride contents in roots and leaves of N. oleander, P. crinitum, and P. oleracea were 1.85, 1.19, and 1.43, and 9.8, 3.6, and 2.2, respectively. P . oleracea, P. crinitum, and N. oleander showed reductions in chlorophyll contents by 40, 57 and 25 and 8%, carbohydrates by 50, 44, and 16%, and proteins by 38, 53, and 15%, respectively. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) in the roots of P. oleracea, P. crinitum, and N. oleander were observed to be induced by 400, 383, and 500%; 80, 105, and 424%; and 153, 77, and 71%, respectively, while the leaves showed induction in SOD, CAT, and GPX activities by 550, 315, and 165%; 196, 227, and 243%; and 280, 242, and 184%, respectively. Results endorsed the superiority of N. oleander for fluoride removal over other plant species. [Mh] Termos MeSH primário: Fluoretos/metabolismo Nerium/metabolismo Poaceae/metabolismo Portulaca/metabolismo Poluentes Químicos da Água/metabolismo [Mh] Termos MeSH secundário: Biodegradação Ambiental Catalase/metabolismo Clorofila/metabolismo Fluoretos/análise Estresse Oxidativo Folhas de Planta/metabolismo Proteínas de Plantas/metabolismo Raízes de Plantas/metabolismo Poluentes Químicos da Água/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Proteins); 0 (Water Pollutants, Chemical); 1406-65-1 (Chlorophyll); EC 1.11.1.6 (Catalase); Q80VPU408O (Fluorides) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 171104 [Lr] Data última revisão: 171104 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170119 [St] Status: MEDLINE [do] DOI: 10.1007/s11356-017-8424-8

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[PMID]: 28077129 [Au] Autor: Ramadan BK; Schaalan MF; Tolba AM [Ad] Endereço: Department of Physiology, Faculty of Medicine for Girls (Cairo), Al-Azhar University, Cairo, Egypt. [Ti] Título: Hypoglycemic and pancreatic protective effects of Portulaca oleracea extract in alloxan induced diabetic rats. [So] Source: BMC Complement Altern Med;17(1):37, 2017 Jan 11. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Diabetes is a major public health concern. In spite of continuous new drug development to treat diabetes, herbal remedies remain a potential adjunct therapy to maintain better glycemic control while also imparting few side-effects. Portulaca oleracea has been traditionally used to manage several diseases due to the anti-oxidant and anti-atherogenic effects it imparts. To better understand the mechanisms associated with potential protective effect of P. oleracea extract against diabetes, alloxan-induced diabetic rats were used in this study. METHODS: Forty Wistar rats (male, 7-8-wk-old, 140-160 g) were divided into four groups (n = 10/group): Group I (control), Group II (P. oleracea-treated; gavaged with P. oleracea extract daily [at 250 mg/kg] for 4 weeks), Group III (diabetic control; daily IP injection of alloxan [at 75 mg/kg] for 5 days) and Group IV (P. oleracea-pre-treated diabetic; gavaged with P. oleracea extract daily [at 250 mg/kg] for 4 weeks and then daily IP injection of alloxan [at 75 mg/kg] for 5 days). Body weight, food consumption, blood (serum) levels of glucose, C peptide, Hb A1C, insulin, tumor necrosis factor (TNF)-α and interleukin (IL)-6 were determined for all groups. RESULTS: The results indicated that while Hb A1C, serum levels of glucose, TNF-α and IL-6 were all significantly decreased in the P. oleracea-pre-treated diabetic rats, these hosts also had significant increases in C peptide and insulin compared to levels in the counterpart diabetic rats. These results were confirmed by the histopathological assessments which showed marked improvement of the destructive effect on pancreatic islet cells induced by alloxan. CONCLUSION: P. oleracea extract is a general tissue protective and regeneartive agent, as evidenced by increasing ß-cell mass and therefore improved the glucose metabolism. Thus, stimulation of Portulaca oleracea signaling in ß- cells may be a novel therapeutic strategy for diabetes prevention. [Mh] Termos MeSH primário: Diabetes Mellitus Experimental/tratamento farmacológico Hipoglicemiantes/administração & dosagem Pâncreas/efeitos dos fármacos Extratos Vegetais/administração & dosagem Portulaca/química Substâncias Protetoras/administração & dosagem [Mh] Termos MeSH secundário: Aloxano Animais Glicemia/metabolismo Diabetes Mellitus Experimental/sangue Diabetes Mellitus Experimental/induzido quimicamente Hemoglobina A Glicada/metabolismo Seres Humanos Insulina/sangue Interleucina-6/sangue Fígado/efeitos dos fármacos Fígado/metabolismo Masculino Pâncreas/metabolismo Fitoterapia Ratos Ratos Wistar Fator de Necrose Tumoral alfa/sangue [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Blood Glucose); 0 (Glycated Hemoglobin A); 0 (Hypoglycemic Agents); 0 (Insulin); 0 (Interleukin-6); 0 (Plant Extracts); 0 (Protective Agents); 0 (Tumor Necrosis Factor-alpha); 6SW5YHA5NG (Alloxan) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170113 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-016-1530-1

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[PMID]: 28041560 [Au] Autor: Delfan-Hosseini S; Nayebzadeh K; Mirmoghtadaie L; Kavosi M; Hosseini SM [Ad] Endereço: Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. [Ti] Título: Effect of extraction process on composition, oxidative stability and rheological properties of purslane seed oil. [So] Source: Food Chem;222:61-66, 2017 May 01. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Purslane seed oil could be considered as potential nutritious oil due to its desirable fatty acid composition and other biological active compounds. In this study the effect of three extraction procedure including solvent extraction, cold pressing and microwave pretreatment (MW) followed by cold pressing on oil yield, physicochemical properties, oxidative stability and rheological behaviors of oil was investigated. Solvent extracted oil had the highest extraction yield (72.31%). Pretreatment by microwave before cold press extraction resulted in an increase in extraction yield, total phenolic compound (TPC) and antioxidant activity. Cold press extracted oil had the lowest oxidative stability (4.64h). This property was greatly enhanced by microwave irradiation, so that the longest oxidative stability was found in MW-cold press extracted oil with 9.67h. Furthermore, all extracted oils demonstrated Newtonian flow behaviors. MW-cold press extracted oil had the greatest apparent viscosity and highest sensitivity to temperature changes (E =29.18kJ/mol ). [Mh] Termos MeSH primário: Óleos Vegetais/química Portulaca/química [Mh] Termos MeSH secundário: Antioxidantes/farmacologia Ácidos Graxos/análise Micro-Ondas Fenóis/análise Sementes/química Viscosidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Fatty Acids); 0 (Phenols); 0 (Plant Oils) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170103 [St] Status: MEDLINE

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[PMID]: 27936895 [Au] Autor: Vijayaraghavan K; Arockiaraj J; Kamala-Kannan S [Ad] Endereço: a Department of Chemical Engineering , Indian Institute of Technology Madras , Chennai , Tamil Nadu , India. [Ti] Título: Portulaca grandiflora as green roof vegetation: Plant growth and phytoremediation experiments. [So] Source: Int J Phytoremediation;19(6):537-544, 2017 Jun 03. [Is] ISSN: 1549-7879 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Finding appropriate rooftop vegetation may improve the quality of runoff from green roofs. Portulaca grandiflora was examined as possible vegetation for green roofs. Green roof substrate was found to have low bulk density (360.7 kg/m ) and high water-holding capacity (49.4%), air-filled porosity (21.1%), and hydraulic conductivity (5270 mm/hour). The optimal substrate also supported the growth of P. grandiflora with biomass multiplication of 450.3% and relative growth rate of 0.038. Phytoextraction potential of P. grandiflora was evaluated using metal-spiked green roof substrate as a function of time and spiked substrate metal concentration. It was identified that P. grandiflora accumulated all metals (Al, Cd, Cr, Cu, Fe, Ni, Pb, and Zn) from metal-spiked green roof substrate. At the end of 40 days, P. grandiflora accumulated 811 ± 26.7, 87.2 ± 3.59, 416 ± 15.8, 459 ± 15.6, 746 ± 20.9, 357 ± 18.5, 565 ± 6.8, and 596 ± 24.4 mg/kg of Al, Cd, Cr, Cu, Fe, Ni, Pb and Zn, respectively. Results also indicated that spiked substrate metal concentration strongly influenced metal accumulation property of P. grandiflora with metal uptake increased and accumulation factor decreased with increase in substrate metal concentration. P. grandiflora also showed potential to translocate all the examined metals with translocation factor greater than 1 for Al, Cu, Fe, and Zn, indicating hyperaccumulation property. [Mh] Termos MeSH primário: Alumínio/metabolismo Metais Pesados/metabolismo Portulaca/metabolismo Poluentes do Solo/metabolismo [Mh] Termos MeSH secundário: Biodegradação Ambiental Índia Portulaca/crescimento & desenvolvimento Chuvas/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Metals, Heavy); 0 (Soil Pollutants); CPD4NFA903 (Aluminum) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170406 [Lr] Data última revisão: 170406 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161213 [St] Status: MEDLINE [do] DOI: 10.1080/15226514.2016.1267699

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[PMID]: 27889331 [Au] Autor: Noorbakhshnia M; Karimi-Zandi L [Ad] Endereço: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran. Electronic address: mnoorbakhshnia@yahoo.com. [Ti] Título: Portulaca oleracea L. prevents lipopolysaccharide-induced passive avoidance learning and memory and TNF-α impairments in hippocampus of rat. [So] Source: Physiol Behav;169:69-73, 2017 Feb 01. [Is] ISSN: 1873-507X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: There is a growing body of evidence that neuroinflammation can impair memory. It has been indicated that Portulaca oleracea Linn. (POL), possess anti-inflammatory activity and might improve memory disruption caused by inflammation. In this study the effect of pre-treatment with the hydro-alcoholic extract of POL on memory retrieval investigated in lipopolysaccharide (LPS) treated rats. Male Wistar rats (200-220g) received either a control diet or a diet containing of POL (400mg/kg, p.o.) for 14days. Then, they received injections of either saline or LPS (1mg/kg, i.p.). In all the experimental groups, 4h following the last injection, passive avoidance learning (PAL) and memory test was performed. The retention test was done 24h after the training and then the animals were sacrificed. Hippocampal TNF-α levels measured using ELISA as one criteria of LPS-induced neuroinflammation. The results indicated that LPS significantly impaired PAL and memory and increased TNF-α levels in hippocampus tissue. Pre-treatment with POL improved memory in control rats and prevented memory and TNF-α deterioration in LPS treated rats. Taken together, the results of this study suggest that the hydro-alcoholic extract of POL may improve memory deficits in LPS treated rats, possibly via inhibition of TNF-α and anti-inflammatory activity. [Mh] Termos MeSH primário: Aprendizagem da Esquiva/efeitos dos fármacos Hipocampo/efeitos dos fármacos Transtornos de Aprendizagem/prevenção & controle Extratos Vegetais/uso terapêutico Portulaca/química Fator de Necrose Tumoral alfa/metabolismo [Mh] Termos MeSH secundário: Análise de Variância Animais Modelos Animais de Doenças Relação Dose-Resposta a Droga Hipocampo/metabolismo Transtornos de Aprendizagem/induzido quimicamente Lipopolissacarídeos/toxicidade Masculino Ratos Ratos Wistar [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Lipopolysaccharides); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170628 [Lr] Data última revisão: 170628 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161128 [St] Status: MEDLINE

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[PMID]: 27806650 [Au] Autor: Xu L; Ying Z; Wei W; Hao D; Wang H; Zhang W; Li C; Jiang M; Ying X; Liu J [Ad] Endereço: a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China. [Ti] Título: A novel alkaloid from Portulaca oleracea L. [So] Source: Nat Prod Res;31(8):902-908, 2017 Apr. [Is] ISSN: 1478-6427 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A novel alkaloid named oleraciamide C (1), with six known compounds, hydroxydihydrobovolide (2), uracil (3), catechol (4), 4-aminophenol (5), vanillic acid (6) as well as 3-hydroxypyridine (7), were isolated from Portulaca oleracea L. Additionally, hydroxydihydrobovolide (2), 4-aminophenol (5), 3-hydroxypyridine (7) were obtained from the plant for the first time. Structure of the new compound was determined using spectroscopic methods including HR-ESI-TOF-MS, 1D and 2D NMR. Others were elucidated through H NMR, C NMR spectra and comparison with literature data. Notably, Compound 1 possessed an unusual bis-substituted eight-membered ring linked with the ß-glucopyranose moiety. The cytotoxicity of compound 1 was evaluated against human adipose-derived stem cells (hADSCs) by CCK-8 method. [Mh] Termos MeSH primário: Alcaloides/química Alcaloides/farmacologia Monossacarídeos/química Portulaca/química [Mh] Termos MeSH secundário: Tecido Adiposo/citologia Alcaloides/isolamento & purificação Aminofenóis/isolamento & purificação Glicosídeos Cardíacos/química Catecóis/isolamento & purificação Sobrevivência Celular/efeitos dos fármacos Células Cultivadas Avaliação Pré-Clínica de Medicamentos/métodos Seres Humanos Espectroscopia de Ressonância Magnética Estrutura Molecular Monossacarídeos/farmacologia Plantas Medicinais/química Piridinas/isolamento & purificação Espectrometria de Massas por Ionização por Electrospray Células-Tronco/efeitos dos fármacos Ácido Vanílico/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Alkaloids); 0 (Aminophenols); 0 (Cardiac Glycosides); 0 (Catechols); 0 (Monosaccharides); 0 (Pyridines); 0 (hydroxydihydrobovolide); 0 (oleraciamide C); 4KBE4P5B6S (3-hydroxypyridine); GM8Q3JM2Y8 (Vanillic Acid); LF3AJ089DQ (catechol); R7P8FRP05V (4-aminophenol) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161104 [St] Status: MEDLINE [do] DOI: 10.1080/14786419.2016.1253081

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[PMID]: 27699650 [Au] Autor: D'Imperio M; Brunetti G; Gigante I; Serio F; Santamaria P; Cardinali A; Colucci S; Minervini F [Ad] Endereço: Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), Bari, Italy. [Ti] Título: Integrated in vitro approaches to assess the bioaccessibility and bioavailability of silicon-biofortified leafy vegetables and preliminary effects on bone. [So] Source: In Vitro Cell Dev Biol Anim;53(3):217-224, 2017 Mar. [Is] ISSN: 1543-706X [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Food industries are increasingly oriented toward new foods to improve nutritional status and/or to combat nutritional deficiency diseases. In this context, silicon biofortification could be an innovative tool for obtaining new foods with possible positive effects on bone mineralization. In this paper, an alternative and quick in vitro approach was applied in order to evaluate the potential health-promoting effects of five silicon-biofortified leafy vegetables (tatsoi, mizuna, purslane, Swiss chard and chicory) on bone mineralization compared with a commercial silicon supplement. The silicon bioaccessibility and bioavailability of the five leafy vegetables (biofortified or not) and of the supplement were assessed by applying a protocol consisting of in vitro gastrointestinal digestion coupled with a Caco-2 cell model. Silicon bioaccessibility ranged from 0.89 to 8.18 mg/L and bioavailability ranged from 111 to 206 µg/L of Si for both vegetables and supplement. Furthermore, the bioavailable fractions were tested on a human osteoblast cell model following the expression of type 1 collagen and alkaline phosphatase. The results obtained highlighted that the bioavailable fraction of biofortified purslane and Swiss chard improved the expression of both osteoblast markers compared with the supplement and other vegetables. These results underline the potentially beneficial effect of biofortified leafy vegetables and also indicate the usefulness of in vitro approaches for selecting the best vegetable with positive bone effects for further in vivo research. [Mh] Termos MeSH primário: Biofortificação Suplementos Nutricionais Digestão/efeitos dos fármacos Silício/administração & dosagem [Mh] Termos MeSH secundário: Disponibilidade Biológica Células CACO-2 Calcificação Fisiológica/efeitos dos fármacos Chicória/química Seres Humanos Osteoblastos/efeitos dos fármacos Folhas de Planta/química Portulaca/química Silício/química Verduras/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: Z4152N8IUI (Silicon) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 171105 [Lr] Data última revisão: 171105 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161005 [St] Status: MEDLINE [do] DOI: 10.1007/s11626-016-0100-7

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[PMID]: 28035114 [Au] Autor: Eskandari EG; Doudi M; Abedi S [Ad] Endereço: Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. [Ti] Título: An study of antileishmanial effect of extract. [So] Source: J Vector Borne Dis;53(4):362-369, 2016 Oct-Dec. [Is] ISSN: 0972-9062 [Cp] País de publicação: India [La] Idioma: eng [Ab] Resumo: BACKGROUND & OBJECTIVES: Leishmaniasis is caused by protozoa of Leishmania genus and is considered as a zoonotic disease. It is a major public health problem worldwide, with high endemicity in developing countries like Iran. Various chemical drugs are used for leishmaniasis treatment, but their side-effects and the emergence of drug resistance have led to look for new effective compounds. The aim of this study was to introduce purslane (Portulaca oleracea) as a traditional and medicinal herb which might act as a valuable source for designing new pharmaceutical drug/lead against Leishmania sp. METHODS: This study was conducted in the laboratory of Seddigheh Tahereh Infectious Disease Research Center, Isfahan, Iran during the spring of 2015. The essence from the purslane plant was prepared through water distillation and the alcoholic extract was prepared through maceration method. The essence was dried, and diluted with DMSO (5%). Leishmania major promastigotes were cultured in 25 Î� 2΀C temperature in the stationary phase of RPMI-1640 medium, enriched with 10% fetal calf serum and penicillin-streptomycin to yield higher quantity. The biological activity of herb essence was evaluated on L. major promastigotes and compared to glucantime reference drug using methylthiazole tetrazolium (MTT) colorometric assay. The optical density absorbance was measured with Eliza reader set, and the IC50 value was calculated at different time intervals. All tests were repeated thrice. Results were analyzed by using Tukey test and t-test. RESULTS: The IC50 values after 48 h, for glucantime against standard parasite promastigotes and clinical strains were equal to 12 and 19 mg/ml, respectively, whereas for purslane herb leaves and stems essence; it was equal to 360 and 680 mg/ml, respectively. Although, the glucantime pharmaceutical drug was more efficient compared to the investigated herb essence, the essense had significant effect on L. major promastigotes with increasing density (p <0.05). The ingredients of the herb leaves and stem essence were-Phytol, squalene, palmitic acid, ethyl- linoleate, ferulic acid, linolenic acid, scopoletin, linoleic acid, rhein, apigenin, and bergapten. INTERPRETATION & CONCLUSION: The study showed that essence of purslane has considerable antileishmanial effects and can stop the growth of parasites in the laboratory compared to glucantime. More experiments are necessary to investigate its effect on Leishmania parasite in animal model. [Mh] Termos MeSH primário: Antiprotozoários/farmacologia Leishmania major/efeitos dos fármacos Extratos Vegetais/farmacologia Portulaca/química [Mh] Termos MeSH secundário: Antiprotozoários/isolamento & purificação Sobrevivência Celular/efeitos dos fármacos Colorimetria/métodos Formazans/análise Concentração Inibidora 50 Irã (Geográfico) Testes de Sensibilidade Parasitária Extratos Vegetais/isolamento & purificação Espectrofotometria Sais de Tetrazólio/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antiprotozoal Agents); 0 (Formazans); 0 (Plant Extracts); 0 (Tetrazolium Salts); 23305-68-2 (MTT formazan) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170414 [Lr] Data última revisão: 170414 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161231 [St] Status: MEDLINE

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