Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.200.800 [Categoria DeCS]
Referências encontradas : 2930 [refinar]
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  1 / 2930 MEDLINE  
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[PMID]:28462718
[Au] Autor:Perlikowska R; Janecka A
[Ad] Endereço:Department of Biomolecular Chemistry, Faculty of Medicine, Medical University of Lodz, 92-215 Lodz. Poland.
[Ti] Título:Rubiscolins - Highly Potent Peptides Derived from Plant Proteins.
[So] Source:Mini Rev Med Chem;18(2):104-112, 2018.
[Is] ISSN:1875-5607
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rubiscolins are two naturally occurring, linear peptides, isolated from the pepsin digests of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) from spinach leaves. Their sequences are as follows: Tyr-Pro-Leu-Asp-Leu (YPLDL, rubiscolin-5) and Tyr-Pro-Leu-Asp-Leu-Phe (YPLDLF, rubiscolin-6, also known as rubixyl). Though their structure does not resemble typical opioid peptides, rubiscolins were found to exhibit high affinity and selectivity for the delta opioid receptor and an antinociceptive effect in mice after oral administration. Moreover, orally administered rubiscolin-6 was shown to possess orexigenic, anxiolytic-like, and memory-enhancing activities in mice. This review summarizes various biological activities of rubiscolins in mammalian organism and recent developments on the structure-activity relationship of rubiscolin analogs, aimed at improving their pharmacological properties. Naturally occurring substances, such as rubiscolins, may provide a rational and powerful approach in the design of new therapeutics or functional foods.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
Produtos Biológicos/farmacologia
Dor/tratamento farmacológico
Peptídeos/farmacologia
Proteínas de Plantas/química
Spinacia oleracea/química
[Mh] Termos MeSH secundário: Analgésicos/administração & dosagem
Analgésicos/química
Animais
Produtos Biológicos/administração & dosagem
Produtos Biológicos/química
Seres Humanos
Peptídeos/administração & dosagem
Peptídeos/química
Receptores Opioides delta/antagonistas & inibidores
Receptores Opioides delta/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Analgesics); 0 (Biological Products); 0 (Peptides); 0 (Plant Proteins); 0 (Receptors, Opioid, delta)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.2174/1389557517666170426160703


  2 / 2930 MEDLINE  
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[PMID]:27770413
[Au] Autor:Stine KJ; Jefferson K; Shulga OV
[Ad] Endereço:Department of Chemistry and Biochemistry, Center for Nanoscience, University of Missouri-Saint Louis, One University Boulevard, Saint Louis, MO, 63121-4400, USA. kstine@umsl.edu.
[Ti] Título:Nanoporous Gold for Enzyme Immobilization.
[So] Source:Methods Mol Biol;1504:37-60, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nanoporous gold (NPG) is a material of emerging interest for immobilization of biomolecules, especially enzymes. The material provides a high surface area form of gold that is suitable for physisorption or for covalent modification by self-assembled monolayers. The material can be used as a high surface area electrode and with immobilized enzymes can be used for amperometric detection schemes. NPG can be prepared in a variety of formats from alloys containing between 20 and 50 % atomic composition of gold and less noble element(s) by dealloying procedures. Materials resembling NPG can be prepared by hydrothermal and electrodeposition methods. Related high surface area gold structures have been prepared using templating approaches. Covalent enzyme immobilization can be achieved by first forming a self-assembled monolayer on NPG bearing a terminal reactive functional group followed by conjugation to the enzyme through amide linkages to lysine residues. Enzymes can also be entrapped by physisorption or immobilized by electrostatic interactions.
[Mh] Termos MeSH primário: Enzimas Imobilizadas/química
Ouro/química
Nanoporos/ultraestrutura
[Mh] Termos MeSH secundário: Acetilcolinesterase/química
Animais
Técnicas Biossensoriais
Técnicas Eletroquímicas
Eletrodos
Estabilidade Enzimática
Glucose Oxidase/química
Peroxidase do Rábano Silvestre/química
Seres Humanos
Imunoconjugados/química
Lacase/química
Complexo de Proteína do Fotossistema I/química
Porosidade
Spinacia oleracea/enzimologia
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes, Immobilized); 0 (Immunoconjugates); 0 (Photosystem I Protein Complex); 7440-57-5 (Gold); EC 1.1.3.4 (Glucose Oxidase); EC 1.10.3.2 (Laccase); EC 1.11.1.- (Horseradish Peroxidase); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


  3 / 2930 MEDLINE  
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[PMID]:29191002
[Au] Autor:Nain-Perez A; Barbosa LCA; Maltha CRA; Giberti S; Forlani G
[Ad] Endereço:Department of Chemistry, Universidade Federal de Minas Gerais , Av. Pres. Antônio Carlos, 6627, Campus Pampulha, CEP 31270-901, Belo Horizonte, MG Brazil.
[Ti] Título:Tailoring Natural Abenquines To Inhibit the Photosynthetic Electron Transport through Interaction with the D1 Protein in Photosystem II.
[So] Source:J Agric Food Chem;65(51):11304-11311, 2017 Dec 27.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Abenquines are natural N-acetylaminobenzoquinones bearing amino acid residues, which act as weak inhibitors of the photosynthetic electron transport chain. Aiming to exploit the abenquine scaffold as a model for the synthesis of new herbicides targeting photosynthesis, 14 new analogues were prepared by replacing the amino acid residue with benzylamines and the acetyl with different acyl groups. The synthesis was accomplished in three steps with a 68-95% overall yield from readily available 2,5-dimethoxyaniline, acyl chlorides, and benzyl amines. Key steps include (i) acylation of the aniline, (ii) oxidation, and (iii) oxidative addition of the benzylamino moiety. The compounds were assayed for their activity as Hill inhibitors, under basal, uncoupled, or phosphorylating conditions, or excluding photosystem I. Four analogues showed high effectiveness (IC = 0.1-0.4 µM), comparable with the commercial herbicide diuron (IC = 0.3 µM). The data suggest that this class of compounds interfere at the reducing side of photosystem II, having protein D1 as the most probable target. Molecular docking studies with the plastoquinone binding site of Spinacia oleracea further strengthened this proposal.
[Mh] Termos MeSH primário: Benzoquinonas/farmacologia
Transporte de Elétrons/efeitos dos fármacos
Herbicidas/farmacologia
Fotossíntese/efeitos dos fármacos
Complexo de Proteína do Fotossistema II/metabolismo
Spinacia oleracea/metabolismo
[Mh] Termos MeSH secundário: Benzoquinonas/química
Cloroplastos/efeitos dos fármacos
Cloroplastos/metabolismo
Herbicidas/química
Simulação de Acoplamento Molecular
Spinacia oleracea/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoquinones); 0 (Herbicides); 0 (Photosystem II Protein Complex)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04624


  4 / 2930 MEDLINE  
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[PMID]:29240839
[Au] Autor:Tome-Stangl L; Schaetzel C; Tenzer S; Bernhard F; Schneider D
[Ad] Endereço:Institute of Pharmacy and Biochemistry, Johannes Gutenberg-University Mainz, Mainz, Germany.
[Ti] Título:The soluble loop BC region guides, but not dictates, the assembly of the transmembrane cytochrome b6.
[So] Source:PLoS One;12(12):e0189532, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Studying folding and assembly of naturally occurring α-helical transmembrane proteins can inspire the design of membrane proteins with defined functions. Thus far, most studies have focused on the role of membrane-integrated protein regions. However, to fully understand folding pathways and stabilization of α-helical membrane proteins, it is vital to also include the role of soluble loops. We have analyzed the impact of interhelical loops on folding, assembly and stability of the heme-containing four-helix bundle transmembrane protein cytochrome b6 that is involved in charge transfer across biomembranes. Cytochrome b6 consists of two transmembrane helical hairpins that sandwich two heme molecules. Our analyses strongly suggest that the loop connecting the helical hairpins is not crucial for positioning the two protein "halves" for proper folding and assembly of the holo-protein. Furthermore, proteolytic removal of any of the remaining two loops, which connect the two transmembrane helices of a hairpin structure, appears to also not crucially effect folding and assembly. Overall, the transmembrane four-helix bundle appears to be mainly stabilized via interhelical interactions in the transmembrane regions, while the soluble loop regions guide assembly and stabilize the holo-protein. The results of this study might steer future strategies aiming at designing heme-binding four-helix bundle structures, involved in transmembrane charge transfer reactions.
[Mh] Termos MeSH primário: Citocromos b6/metabolismo
Proteínas de Membrana/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Citocromos b6/química
Citocromos b6/genética
Dimerização
Proteínas de Membrana/química
Mutagênese Sítio-Dirigida
Dobramento de Proteína
Proteólise
Spinacia oleracea/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytochromes b6); 0 (Membrane Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189532


  5 / 2930 MEDLINE  
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[PMID]:29190770
[Au] Autor:Shi A; Qin J; Mou B; Correll J; Weng Y; Brenner D; Feng C; Motes D; Yang W; Dong L; Bhattarai G; Ravelombola W
[Ad] Endereço:Department of Horticulture, University of Arkansas, Fayetteville, AR, United States of America.
[Ti] Título:Genetic diversity and population structure analysis of spinach by single-nucleotide polymorphisms identified through genotyping-by-sequencing.
[So] Source:PLoS One;12(11):e0188745, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Spinach (Spinacia oleracea L., 2n = 2x = 12) is an economically important vegetable crop worldwide and one of the healthiest vegetables due to its high concentrations of nutrients and minerals. The objective of this research was to conduct genetic diversity and population structure analysis of a collection of world-wide spinach genotypes using single nucleotide polymorphisms (SNPs) markers. Genotyping by sequencing (GBS) was used to discover SNPs in spinach genotypes. Three sets of spinach genotypes were used: 1) 268 USDA GRIN spinach germplasm accessions originally collected from 30 countries; 2) 45 commercial spinach F1 hybrids from three countries; and 3) 30 US Arkansas spinach cultivars/breeding lines. The results from this study indicated that there was genetic diversity among the 343 spinach genotypes tested. Furthermore, the genetic background in improved commercial F1 hybrids and in Arkansas cultivars/lines had a different structured populations from the USDA germplasm. In addition, the genetic diversity and population structures were associated with geographic origin and germplasm from the US Arkansas breeding program had a unique genetic background. These data could provide genetic diversity information and the molecular markers for selecting parents in spinach breeding programs.
[Mh] Termos MeSH primário: Variação Genética
Genótipo
Polimorfismo de Nucleotídeo Único
Spinacia oleracea/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188745


  6 / 2930 MEDLINE  
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[PMID]:27777365
[Au] Autor:Jeng SC; Chan HH; Booy EP; McKenna SA; Unrau PJ
[Ad] Endereço:Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
[Ti] Título:Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers.
[So] Source:RNA;22(12):1884-1892, 2016 12.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effective tracking and purification of biological RNAs and RNA protein complexes is currently challenging. One promising strategy to simultaneously address both of these problems is to develop high-affinity RNA aptamers against taggable small molecule fluorophores. RNA Mango is a 39-nucleotide, parallel-stranded G-quadruplex RNA aptamer motif that binds with nanomolar affinity to a set of thiazole orange (TO1) derivatives while simultaneously inducing a 10 -fold increase in fluorescence. We find that RNA Mango has a large increase in its thermal stability upon the addition of its TO1-Biotin ligand. Consistent with this thermal stabilization, RNA Mango can effectively discriminate TO1-Biotin from a broad range of small molecule fluorophores. In contrast, RNA Spinach, which is known to have a substantially more rigid G-quadruplex structure, was found to bind to this set of fluorophores, often with higher affinity than to its native ligand, 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI), and did not exhibit thermal stabilization in the presence of the TO1-Biotin fluorophore. Our data suggest that RNA Mango is likely to use a concerted ligand-binding mechanism that allows it to simultaneously bind and recognize its TO1-Biotin ligand, whereas RNA Spinach appears to lack such a mechanism. The high binding affinity and fluorescent efficiency of RNA Mango provides a compelling alternative to RNA Spinach as an RNA reporter system and paves the way for the future development of small fluorophore RNA reporter systems.
[Mh] Termos MeSH primário: Corantes Fluorescentes/química
Mangifera/química
RNA de Plantas/química
Spinacia oleracea/química
[Mh] Termos MeSH secundário: Ligantes
Espectrometria de Fluorescência
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Ligands); 0 (RNA, Plant)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171223
[Lr] Data última revisão:
171223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  7 / 2930 MEDLINE  
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[PMID]:28873632
[Au] Autor:Corte-Real J; Desmarchelier C; Borel P; Richling E; Hoffmann L; Bohn T
[Ad] Endereço:Department of Population Health, Luxembourg Institute of Health (LIH), 1A-B, rue Thomas Edison, L-1445 Strassen, Luxembourg; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41 rue du Brill, L-4422 Belvaux, Luxembourg; Food Chemistry and
[Ti] Título:Magnesium affects spinach carotenoid bioaccessibility in vitro depending on intestinal bile and pancreatic enzyme concentrations.
[So] Source:Food Chem;239:751-759, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Magnesium may reduce carotenoid bioavailability by forming insoluble complexes with bile salts/fatty acids, inhibiting micelle formation. Here, we investigated whether altering bile/pancreatin concentration influenced potential negative effects of magnesium on carotenoid bioaccessibility. Spinach (4g) was digested in vitro with added magnesium (0, 200, 400mg/L) and canola oil/coffee creamer, at varying bile extract (1 or 8mM) and pancreatin (100 or 990mg/L) concentrations. Bioaccessibility was determined for ß-carotene, lutein, and total carotenoids via HPLC. Additionally, lipolysis, particle size, and zeta potential of the micellar fractions were investigated. Increasing magnesium concentrations negatively affected carotenoid bioaccessibility (p<0.001), lipolysis, particle size and zeta potential. The impact of magnesium on carotenoid bioaccessibility was modulated mainly by bile concentration, with samples digested with 1mM of bile being more susceptible to inhibitory effects of magnesium than those digested with 8mM (p<0.001). Thus, magnesium was found to potentially interfere with carotenoid bioaccessibility at various physiologically plausible conditions.
[Mh] Termos MeSH primário: Pâncreas/enzimologia
Spinacia oleracea
[Mh] Termos MeSH secundário: Bile
Disponibilidade Biológica
Carotenoides
Magnésio
beta Caroteno
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
01YAE03M7J (beta Carotene); 36-88-4 (Carotenoids); I38ZP9992A (Magnesium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  8 / 2930 MEDLINE  
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[PMID]:28867103
[Au] Autor:Alozie YE; Ene-Obong HN
[Ad] Endereço:Department of Human Ecology, Nutrition and Dietetics, Faculty of Agriculture, University of Uyo, Akwa Ibom State, Nigeria. Electronic address: tundealozie@yahoo.com.
[Ti] Título:Recipe standardization, nutrient composition and sensory evaluation of waterleaf (Talinum triangulare) and wild spinach (Gnetum africanum) soup "afang" commonly consumed in South-south Nigeria.
[So] Source:Food Chem;238:65-72, 2018 Jan 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:One hundred recipes of waterleaf and wild spinach soup (afang) consumed among the Ibibios in South-south Nigeria were collected through interview and questionnaire from indigenous homemakers and food sellers, harmonized, standardized, prepared and their nutrient content calculated. Mean weights of ingredients were calculated to obtain the control recipe. Major ingredients in the soup were analyzed chemically. Edible portions, retention factors to be applied in recipe calculation were determined. Sensory evaluation was conducted on five of the most preferred recipes on a nine-point hedonic scale. Edible coefficients of major foods ranged between 0.32 and 0.95. Significant changes (p<0.05) in nutrients were observed between raw and cooked ingredients and recipes. Afang soup had 67.9% moisture; protein, 12.7% and energy, 169kcal. Fat contributed 57% of the total energy. Consumption of adequate quantities of afang soup will contribute substantially to Recommended Nutrient Intake of protein and micronutrients which will further increase with additional fish/meat.
[Mh] Termos MeSH primário: Spinacia oleracea
Traqueófitas
[Mh] Termos MeSH secundário: Animais
Culinária
Ingestão de Energia
Seres Humanos
Carne
Nigéria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE


  9 / 2930 MEDLINE  
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[PMID]:28922029
[Au] Autor:Plessis EMD; Govender S; Pillay B; Korsten L
[Ad] Endereço:1 Centre of Excellence in Food Security, Department of Plant and Soil Sciences, University of Pretoria, Hatfield, Pretoria 0002, South Africa; and.
[Ti] Título:Exploratory Study into the Microbiological Quality of Spinach and Cabbage Purchased from Street Vendors and Retailers in Johannesburg, South Africa.
[So] Source:J Food Prot;80(10):1726-1733, 2017 Oct.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Knowledge of the microbiological quality and prevalence of antibiotic resistance and virulence genes in bacterial isolates from leafy green vegetables supplied by formal suppliers (retailers) and informal suppliers (street vendors) in South Africa is limited. Because leafy vegetables have been implicated in foodborne disease outbreaks worldwide, 180 cabbage and spinach samples were collected from three major retailers and nine street vendors in Johannesburg, South Africa. Escherichia coli and coliforms were enumerated using Petrifilm plates. The prevalence of Listeria monocytogenes, Salmonella, and Shigella was determined using real-time PCR analysis. Identities of presumptive E. coli isolates from the fresh produce were confirmed using matrix-assisted laser desorption-ionization time of flight mass spectroscopy. Isolates were characterized using phenotypic (antibiotic resistance) and genotypic (phylogenetic and virulence gene) analysis. Hygiene indicator bacteria levels on spinach from formal and informal retailers exceeded the maximum level specified by the Department of Health guidelines for fresh fruit and vegetables. E. coli counts for street vendor spinach were higher (P < 0.0789) than those for retailer spinach. E. coli was present in only two cabbage samples, at 0.0035 CFU/g. L. monocytogenes and Salmonella were detected in 7.2 and 5% of the 180 samples, respectively, based on real-time PCR analysis; Shigella was not detected. Of the 29 spinach E. coli isolates, 37.9% were multidrug resistant. Virulence genes eae and stx were present in 14 and 3% of the spinach E. coli isolates, respectively; the stx gene was not detected. Eighty-six percent of these isolates belonged to phylogroup A, 3% belonged to group C, 7% belonged to group E, and 3% belonged to clade 1. The results from the current exploratory study on the microbiological quality of spinach bought from selected retailers highlight the need for continued surveillance on a larger scale, especially in the informal sector, to characterize the potential health risks to the consumer.
[Mh] Termos MeSH primário: Brassica/microbiologia
Segurança de Produtos ao Consumidor
Contaminação de Alimentos/análise
Spinacia oleracea/microbiologia
[Mh] Termos MeSH secundário: Escherichia coli
Contaminação de Alimentos/prevenção & controle
Microbiologia de Alimentos
Filogenia
África do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-540


  10 / 2930 MEDLINE  
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[PMID]:28797073
[Au] Autor:Chen HR; Rairat T; Loh SH; Wu YC; Vickroy TW; Chou CC
[Ad] Endereço:Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
[Ti] Título:Assessment of veterinary drugs in plants using pharmacokinetic approaches: The absorption, distribution and elimination of tetracycline and sulfamethoxazole in ephemeral vegetables.
[So] Source:PLoS One;12(8):e0183087, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The present study was carried out to demonstrate novel use of pharmacokinetic approaches to characterize drug behaviors/movements in the vegetables with implications to food safety. The absorption, distribution, metabolism and most importantly, the elimination of tetracycline (TC) and sulfamethoxazole (SMX) in edible plants Brassica rapa chinensis and Ipomoea aquatica grown hydroponically were demonstrated and studied using non-compartmental pharmacokinetic analysis. The results revealed drug-dependent and vegetable-dependent pharmacokinetic differences and indicated that ephemeral vegetables could have high capacity accumulating antibiotics (up to 160 µg g-1 for TC and 38 µg g-1 for SMX) within hours. TC concentration in the root (Cmax) could reach 11 times higher than that in the cultivation fluid and 3-28 times higher than the petioles/stems. Based on the volume of distribution (Vss), SMX was 3-6 times more extensively distributed than TC. Both antibiotics showed evident, albeit slow elimination phase with elimination half-lives ranging from 22 to 88 hours. For the first time drug elimination through the roots of a plant was demonstrated, and by viewing the root as a central compartment and continuous infusion without a loading dose as drug administration mode, it is possible to pharmacokinetically monitor the movement of antibiotics and their fate in the vegetables with more detailed information not previously available. Phyto-pharmacokinetic could be a new area worth developing new models for the assessment of veterinary drugs in edible plants.
[Mh] Termos MeSH primário: Anti-Infecciosos/metabolismo
Brassica rapa/metabolismo
Spinacia oleracea/metabolismo
Sulfametoxazol/metabolismo
Tetraciclina/metabolismo
Verduras/metabolismo
Drogas Veterinárias/metabolismo
[Mh] Termos MeSH secundário: Anti-Infecciosos/análise
Contaminação de Alimentos/análise
Sulfametoxazol/análise
Tetraciclina/análise
Drogas Veterinárias/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Veterinary Drugs); F8VB5M810T (Tetracycline); JE42381TNV (Sulfamethoxazole)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183087



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