Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.233.222 [Categoria DeCS]
Referências encontradas : 70 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 7 ir para página                  

  1 / 70 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28119424
[Au] Autor:Kono M; Yamori W; Suzuki Y; Terashima I
[Ad] Endereço:Department of Biological Sciences, School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.
[Ti] Título:Photoprotection of PSI by Far-Red Light Against the Fluctuating Light-Induced Photoinhibition in Arabidopsis thaliana and Field-Grown Plants.
[So] Source:Plant Cell Physiol;58(1):35-45, 2017 01 01.
[Is] ISSN:1471-9053
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:It has been reported that PSI photoinhibition is induced even in wild-type plants of Arabidopsis thaliana, rice and other species by exposure of leaves to fluctuating light (FL) for a few hours. Because plants are exposed to FL in nature, they must possess protective mechanisms against the FL-induced photodamage. Here, using A. thaliana grown at various irradiances, we examined PSI photoprotection by far-red (FR) light at intensities comparable with those observed in nature. Dark-treated leaves were illuminated by red FL alternating high/low light at 1,200/30 µmol m-2 s-1 for 800 ms/10 s. By this FL treatment without FR light for 120 min, the level of photo-oxidizable P700 was decreased by 30% even in the plants grown at high irradiances. The addition of continuous FR light during the FL suppressed this damage almost completely. With FR light, P700 was kept in a more oxidized state in both low- and high-light phases. The protective effect of FR light was diminished more in mutants of the NADH dehydrogenase-like complex (NDH)-mediated cyclic electron flow around PSI (CEF-PSI) than in the PGR5 (proton gradient regulation 5)-mediated CEF-PSI, indicating that the NDH-mediated CEF-PSI would be a major contributor to PSI photoprotection in the presence of FR light. We also confirmed that PSI photoinhibition decreased with the increase in growth irradiance in A. thaliana and field-grown plants, and that this PSI photodamage was largely suppressed by addition of FR light. These results clearly indicate that the most effective PSI protection is realized in the presence of FR light.
[Mh] Termos MeSH primário: Arabidopsis/efeitos da radiação
Commelina/efeitos da radiação
Erigeron/efeitos da radiação
Luz
Fotossíntese/efeitos da radiação
Complexo de Proteína do Fotossistema I/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Commelina/crescimento & desenvolvimento
Commelina/metabolismo
Erigeron/crescimento & desenvolvimento
Erigeron/metabolismo
Complexo de Proteína do Fotossistema II/metabolismo
Especificidade da Espécie
Luz Solar
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosystem I Protein Complex); 0 (Photosystem II Protein Complex)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170723
[Lr] Data última revisão:
170723
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.1093/pcp/pcw215


  2 / 70 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27768529
[Au] Autor:Contiero RL; Biffe DF; Constantin J; de Oliveira RS; Braz GB; Lucio FR; Schleier JJ
[Ad] Endereço:a Agronomy Department , Universidade Estadual de Maringá , Maringá, Paraná , Brazil.
[Ti] Título:Effects of nozzle types and 2,4-D formulations on spray deposition.
[So] Source:J Environ Sci Health B;51(12):888-893, 2016 Dec.
[Is] ISSN:1532-4109
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to evaluate the effects of nozzle types and 2,4-D formulations on spray deposition on different targets. Two field experiments were carried out in a completely randomized design, and treatments were arranged in a factorial scheme. Species in experiment 1 were Sumatran fleabane (Conyza sumatrensis) and Brazil pusley (Richardia brasiliensis) and in experiment 2 were soybeans (Glycine max) and Benghal dayflower (Commelina benghalensis). For both experiments, the first factor corresponded to spray nozzles with different settings (AD 110.015 - 61 and 105 L ha ; AD 015-D - 75 and 146 L ha ; XR 110.0202 - 200 L ha ; and ADIA-D 110.02 - 208 L ha ) and the second factor consisted of two formulations of 2,4-D (amine and choline). The formulation of 2,4-D choline has contained Colex-D™ Technology. Similar or higher spray deposition was observed on the leaves and artificial targets when using 2,4-D choline as compared to the 2,4-D amine formulation, and these differences in deposition were more evident for nozzles applying lower spray volumes. Deposition was more affected by nozzle type when amine formulation was used, compared to choline formulation.
[Mh] Termos MeSH primário: Ácido 2,4-Diclorofenoxiacético/administração & dosagem
Agricultura/instrumentação
Agricultura/métodos
[Mh] Termos MeSH secundário: Brasil
Commelina
Conyza/efeitos dos fármacos
Dimetilaminas/administração & dosagem
Desenho de Equipamento
Plantas Daninhas/efeitos dos fármacos
Distribuição Aleatória
Feijão de Soja
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dimethylamines); 2008-39-1 (2,4-D amine); 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161022
[St] Status:MEDLINE


  3 / 70 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27521948
[Au] Autor:Yang R; Guo F; Li J; Su N; Shao Z; Zan S
[Ad] Endereço:College of Environmental Science and Engineering, Anhui Normal University, Wuhu 241003, China. Electronic address: yangruyi@mail.ahnu.edu.cn.
[Ti] Título:Effect of copper tolerant Elsholtzia splendens on bacterial community associated with Commelina communis on a copper mine spoil.
[So] Source:J Environ Sci (China);46:165-73, 2016 Aug.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Facilitation, or positive plant-plant interaction, has received increasing concern from ecologists over the last two decades. Facilitation may occur through direct mitigation of severe environments or indirect mediation by a third participant from the same or different trophic levels. The copper (Cu) tolerant species Elsholtzia splendens facilitates the establishment and growth of co-occurring Commelina communis through indirect enrichment of microbial activity. However, whether and how E. splendens impacts the microbial community that is associated with C. communis is less known. We characterized the soil bacterial community in the rhizosphere of C. communis in the absence and presence of E. splendens using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and sequencing. The result showed that the richness of the bacterial community increased, but diversity and evenness remained similar, in the presence of E. splendens. Chloroflexi, Acidobacteria and Proteobacteria were the most dominant bacteria. The relative abundance of dominant and minor bacterial groups showed distinctly different responses to E. splendens. Principal component analysis and redundancy analysis indicated that variation of the bacterial community was determined by multiple factors and might be driven by the tested soil parameters collectively, or alternatively changed through plant root exudates or other microorganisms. Our results enhance the understanding of how the bacterial community associated with a beneficiary plant responds to a benefactor plant and suggests that the changes of bacterial community composition may have far-reaching influence on plant-soil feedback and the aboveground plant community in the long run.
[Mh] Termos MeSH primário: Bactérias
Commelina/fisiologia
Cobre/metabolismo
Lamiaceae/fisiologia
Microbiologia do Solo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Biodegradação Ambiental
Mineração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 789U1901C5 (Copper)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170125
[Lr] Data última revisão:
170125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE


  4 / 70 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27437404
[Au] Autor:Balogun FO; Tshabalala NT; Ashafa AO
[Ad] Endereço:Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Private Bag X 13, Phuthaditjhaba 9866, South Africa.
[Ti] Título:Antidiabetic Medicinal Plants Used by the Basotho Tribe of Eastern Free State: A Review.
[So] Source:J Diabetes Res;2016:4602820, 2016.
[Is] ISSN:2314-6753
[Cp] País de publicação:Egypt
[La] Idioma:eng
[Ab] Resumo:Diabetes mellitus (DM) belongs to the group of five leading important diseases causing death globally and remains a major health problem in Africa. A number of factors such as poverty, poor eating habit, and hormonal imbalance are responsible for the occurrence of the disease. It poses a major health challenge in Africa continent today and the prevalence continues to increase at an alarming rate. Various treatment options particularly the usage of herbs have been effective against diabetes because they have no adverse effects. Interestingly, South Africa, especially the Basotho tribe, is blessed with numerous medicinal plants whose usage in the treatment of DM has been effective since the conventional drugs are expensive and often unaffordable. The present study attempted to update the various scientific evidence on the twenty-three (23) plants originating from different parts of the world but widely used by the Sotho people in the management of DM. Asteraceae topped the list of sixteen (16) plant families and remained the most investigated according to this review. Although limited information was obtained on the antidiabetic activities of these plants, it is however anticipated that government parastatals and scientific communities will pay more attention to these plants in future research.
[Mh] Termos MeSH primário: Diabetes Mellitus/tratamento farmacológico
Hipoglicemiantes/uso terapêutico
Preparações de Plantas/uso terapêutico
Plantas Medicinais
[Mh] Termos MeSH secundário: Aloe
Apocynaceae
Asparagus (Planta)
Asteraceae
Cannabis
Commelina
Fabaceae
Seres Humanos
Hypoxidaceae
Hypoxis
Malvaceae
Mimulus
Myricaceae
Rubiaceae
Rumex
África do Sul
Xanthorrhoeaceae
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Hypoglycemic Agents); 0 (Plant Preparations)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE
[do] DOI:10.1155/2016/4602820


  5 / 70 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27275832
[Au] Autor:Ramos-González PL; Chabi-Jesus C; Guerra-Peraza O; Breton MC; Arena GD; Nunes MA; Kitajima EW; Machado MA; Freitas-Astúa J
[Ad] Endereço:Laboratório de Biotecnologia, Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, São Paulo 13490-970, Brazil. plrg1970@gmail.com.
[Ti] Título:Phylogenetic and Molecular Variability Studies Reveal a New Genetic Clade of Citrus leprosis virus C.
[So] Source:Viruses;8(6), 2016 Jun 06.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Citrus leprosis virus C (CiLV-C) causes a severe disease affecting citrus orchards in the Western hemisphere. This study reveals the molecular variability of the virus by analyzing four genomic regions (p29, p15, MP and RNA2-intergenic region) distributed over its two RNAs. Nucleotide diversity (π) values were relatively low but statistically different over the analyzed genes and subpopulations, indicating their distinct evolutionary history. Values of πp29 and πMP were higher than those of πp15 and πRNA2-IR, whereas πMP was increased due to novel discovered isolates phylogenetically clustered in a divergent clade that we called SJP. Isolate BR_SP_SJP_01 RNA1 and RNA2 sequences, clade SJP, showed an identity of 85.6% and 88.4%, respectively, with those corresponding to CiLV-C, the type member of the genus Cilevirus, and its RNA2 5'-proximal region was revealed as a minor donor in a putative inter-clade recombination event. In addition to citrus, BR_SP_SJP_01 naturally infects the weed Commelina benghalensis and is efficiently transmitted by Brevipalpus yothersi mites. Our data demonstrated that negative selection was the major force operating in the evaluated viral coding regions and defined amino acids putatively relevant for the biological function of cilevirus proteins. This work provides molecular tools and sets up a framework for further epidemiological studies.
[Mh] Termos MeSH primário: Citrus/virologia
Filogenia
Doenças das Plantas/virologia
Vírus de Plantas/classificação
Vírus de Plantas/genética
Vírus de RNA/classificação
Vírus de RNA/genética
[Mh] Termos MeSH secundário: Animais
Commelina/virologia
Transmissão de Doença Infecciosa
Genes Virais
Insetos Vetores/virologia
Ácaros/virologia
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE


  6 / 70 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26961292
[Au] Autor:Kuppusamy P; Ichwan SJ; Al-Zikri PN; Suriyah WH; Soundharrajan I; Govindan N; Maniam GP; Yusoff MM
[Ad] Endereço:Mammalian Cell Culture Technology Laboratory, Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang, Malaysia. kpalaselvam@yahoo.co.in.
[Ti] Título:In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina nudiflora L. Aqueous Extract Against HCT-116 Colon Cancer Cells.
[So] Source:Biol Trace Elem Res;173(2):297-305, 2016 Oct.
[Is] ISSN:1559-0720
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recently, metal nanoparticles have been getting great medical and social interests due to their potential physico-chemical properties such as higher affinity, low molecular weight, and larger surface area. The biosynthesized gold and silver nanoparticles are spherical, triangular in shape with an average size of 24-150 nm as reported in our earlier studies. The biological properties of synthesized gold and silver nanoparticles are demonstrated in this paper. The different in vitro assays such as MTT, flow cytometry, and reverse transcription polymerase chain reaction (RT-qPCR) techniques were used to evaluate the in vitro anticancer properties of synthesized metal nanoparticles. The biosynthesized gold and silver nanoparticles have shown reduced cell viability and increased cytotoxicity in HCT-116 colon cancer cells with IC50 concentration of 200 and 100 µg/ml, respectively. The flow cytometry experiments revealed that the IC50 concentrations of gold and silver nanoparticle-treated cells that have significant changes were observed in the sub-G1 cell cycle phase compared with the positive control. Additionally, the relative messenger RNA (mRNA) gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes such as PUMA (++), Caspase-3 (+), Caspase-8 (++), and Caspase-9 (++) were upregulated in the treated HCT-116 cells compared with cisplatin. Overall, these findings have proved that the synthesized gold and silver nanoparticles could be potent anti-colon cancer drugs.
[Mh] Termos MeSH primário: Antineoplásicos
Neoplasias do Colo/tratamento farmacológico
Commelina/química
Ouro
Nanopartículas Metálicas/química
Extratos Vegetais/química
Prata
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Antineoplásicos/farmacologia
Linhagem Celular Tumoral
Neoplasias do Colo/metabolismo
Neoplasias do Colo/patologia
Ouro/química
Ouro/farmacologia
Seres Humanos
Prata/química
Prata/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Extracts); 3M4G523W1G (Silver); 7440-57-5 (Gold)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160311
[St] Status:MEDLINE
[do] DOI:10.1007/s12011-016-0666-7


  7 / 70 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26927135
[Au] Autor:Yam RS; Fan YT; Wang TT
[Ad] Endereço:Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei 106, Taiwan. ritayam@ntu.edu.tw.
[Ti] Título:Importance of Macrophyte Quality in Determining Life-History Traits of the Apple Snails Pomacea canaliculata: Implications for Bottom-Up Management of an Invasive Herbivorous Pest in Constructed Wetlands.
[So] Source:Int J Environ Res Public Health;13(3), 2016 Feb 24.
[Is] ISSN:1660-4601
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Pomacea canaliculata (Ampullariidae) has extensively invaded most Asian constructed wetlands and its massive herbivory of macrophytes has become a major cause of ecosystem dysfunctioning of these restored habitats. We conducted non-choice laboratory feeding experiments of P. canaliculata using five common macrophyte species in constructed wetlands including Ipomoea aquatica, Commelina communis, Nymphoides coreana, Acorus calamus and Phragmites australis. Effects of macrophytes on snail feeding, growth and fecundity responses were evaluated. Results indicated that P. canaliculata reared on Ipomoea had the highest feeding and growth rates with highest reproductive output, but all individuals fed with Phragmites showed lowest feeding rates and little growth with poorest reproductive output. Plant N and P contents were important for enhancing palatability, supporting growth and offspring quantity of P. canaliculata, whilst toughness, cellulose and phenolics had critically deterrent effects on various life-history traits. Although snail offspring quality was generally consistent regardless of maternal feeding conditions, the reduced growth and offspring quantity of the poorly-fed snails in constructed wetlands dominated by the less-palatable macrophytes could limit the invasive success of P. canaliculata. Effective bottom-up control of P. canaliculata in constructed wetlands should involve selective planting strategy using macrophytes with low nutrient and high toughness, cellulose and phenolic contents.
[Mh] Termos MeSH primário: Comportamento Alimentar/fisiologia
Herbivoria/fisiologia
Espécies Introduzidas
Plantas Daninhas/parasitologia
Caramujos/fisiologia
[Mh] Termos MeSH secundário: Acorus
Animais
Commelina
Ecologia
Ipomoea
Taiwan
Zonas Úmidas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE


  8 / 70 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25058285
[Au] Autor:Ushimaru A; Kobayashi A; Dohzono I
[Ad] Endereço:Graduate School of Human Development and Environment, Kobe University, 3-11 Tsurukabuto, Nada, Kobe 657-8501, Japan.
[Ti] Título:Does urbanization promote floral diversification? Implications from changes in herkogamy with pollinator availability in an urban-rural area.
[So] Source:Am Nat;184(2):258-67, 2014 Aug.
[Is] ISSN:1537-5323
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although land-use changes such as urbanization have dramatically altered plant-pollinator interactions, little is known about their effects on pollen limitation and floral traits. In this study, we examined pollinator visit frequency, reproductive success, and floral trait measurements in 12 populations of the annual andromonoecious Commelina communis in an urban-rural area. Pollinator and mate availability decreased significantly with developed land area around the study site. Most urbanized populations suffered from significant pollinator-limited male and/or female reproductive success. High fruit set in urbanized populations may suggest the presence of high reproductive assurance by selfing. The stigma height and degree of herkogamy significantly decreased with increased pollinator limitation. Petal length, anther height, and/or the pollen:ovule ratio tended to be low in pollinator- and mate-limited urban populations. One urban population with high pollinator availability had flowers with higher herkogamy and stigma height compared to rural populations. These results suggest that urbanization may provide diverse selective forces that could affect the phenotypic variation in floral traits.
[Mh] Termos MeSH primário: Commelina/anatomia & histologia
Commelina/fisiologia
Polinização
Reprodução/fisiologia
Autofertilização
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Flores/anatomia & histologia
Flores/fisiologia
Insetos
Japão
Fenótipo
Urbanização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1509
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140725
[St] Status:MEDLINE
[do] DOI:10.1086/676855


  9 / 70 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24581381
[Au] Autor:Kokilavani P; Suriyakalaa U; Elumalai P; Abirami B; Ramachandran R; Sankarganesh A; Achiraman S
[Ad] Endereço:Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
[Ti] Título:Antioxidant mediated ameliorative steroidogenesis by Commelina benghalensis L. and Cissus quadrangularis L. against quinalphos induced male reproductive toxicity.
[So] Source:Pestic Biochem Physiol;109:18-33, 2014 Feb.
[Is] ISSN:1095-9939
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quinalphos (QP) is speculated to cause endocrine disruption through the generation of reactive oxygen species (ROS) by oxidative stress (OS). Exposure of QP decreased testosterone level considerably which resulted in reduced viable sperms in mice. The QP induced toxicity is initiated by the formation of free radicals as it is evidenced from the increased Lipid peroxidation (LPO) and diminution of antioxidant enzymes in testicular tissue. Increased serum cholesterol and reduced testicular cholesterol indicated the inhibition of cholesterol transport and biosynthesis in testicular tissues. Lack of cholesterol in testicular tissue impaired the steroidogenesis by down-regulating the expression of StAR protein, Cytochrome P450, 3ß-HSD and 17ß-HSD leading to reduced testosterone level. Treatment of Commelina benganlensis (CBE) and Cissus quadrangularis (CQE) significantly recovered the alterations in antioxidant profiles as well as increased LPO, thereby recovering the decreased mRNA expression levels of intermediate enzymes. However, CQE effectively protected the OS and prevented the inhibition of steroidogenesis thereby preventing male infertility.
[Mh] Termos MeSH primário: Cissus
Commelina
Inseticidas/toxicidade
Compostos Organotiofosforados/toxicidade
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: 17-Hidroxiesteroide Desidrogenases/genética
17-Hidroxiesteroide Desidrogenases/metabolismo
3-Hidroxiesteroide Desidrogenases/genética
3-Hidroxiesteroide Desidrogenases/metabolismo
Animais
Catalase/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Colesterol/sangue
Colesterol/metabolismo
Sistema Enzimático do Citocromo P-450/genética
Glutationa Peroxidase/metabolismo
L-Lactato Desidrogenase/metabolismo
Hormônio Luteinizante/sangue
Masculino
Camundongos
Fosfoproteínas/genética
Contagem de Espermatozoides
Espermatozoides/efeitos dos fármacos
Superóxido Dismutase/metabolismo
Testículo/efeitos dos fármacos
Testículo/metabolismo
Testosterona/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insecticides); 0 (Organothiophosphorus Compounds); 0 (Phosphoproteins); 0 (Plant Extracts); 0 (steroidogenic acute regulatory protein); 26S837727Y (quinalphos); 3XMK78S47O (Testosterone); 9002-67-9 (Luteinizing Hormone); 9035-51-2 (Cytochrome P-450 Enzyme System); 97C5T2UQ7J (Cholesterol); EC 1.1.- (17-Hydroxysteroid Dehydrogenases); EC 1.1.- (3-Hydroxysteroid Dehydrogenases); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140304
[St] Status:MEDLINE


  10 / 70 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:24555704
[Au] Autor:Lebogo KW; Mokgotho MP; Bagla VP; Matsebatlela TM; Mbazima V; Shai LJ; Mampuru L
[Ti] Título:Semi-purified extracts of Commelina benghalensis (Commelinaceae) induce apoptosis and cell cycle arrest in Jurkat-T cells.
[So] Source:BMC Complement Altern Med;14:65, 2014 Feb 20.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Commelina benghalensis (CB) is a small plant whose fleshy stems are used in South Africa to treat skin conditions (e.g., cancerous skin outgrowths). This study was aimed at evaluating the effect of sub-fractions of acetone extracts of CB stems on growth-associated molecular events of apoptosis and cell division cycle of Jurkat-T (JT) cells. METHODS: Acetone extract of CB stems were subfractioned into n-hexane (F1) and dichloromethane (F2) fractions. After treatment of JT cells with these subfractions, cell proliferation, viability and apoptosis were determined using a haemocytometer, the trypan blue dye exclusion assay, and Hoechst 33258 staining, respectively. Cell division cycle distribution profiles were analysed using an Epics Alba Flow Cytometer and the expression of cell division cycle regulatory genes was analysed using RT-PCR, while immunoreactive proteins were detected on western blots. RESULTS: The F1 and F2 fractions inhibited the proliferation and viability of JT cells in a concentration- and time-dependent manner, with IC50 values of 32.5 µg/mâ„“ and 56 µg/mâ„“, respectively. The observed cytotoxicity was established to be a consequence of apoptosis. as verified using Hoechst staining method. Both fractions induced a G1/S interphase arrest of the cell division cycle of JT cells.RT-PCR analyses showed an up-regulatory effect by the F1 fraction in the expression of cyclin B1, cdc2 and bax, with a down-regulatory effect in the expression levels of bcl-2. Fraction F1 also increased the protein expression levels of p53 and its downstream regulators, p21 and Cdc2. However, protein Bax and p21 and p53 transcripts were undetectable under the same experimental conditions. On the other hand, fraction F2 increased the mRNA expression levels of bax, bcl-2, cyclin B1 and cdc2. Concomitantly, fraction F2 showed an up-regulation in the protein expression levels of Cdc2, Bcl-2, Cyclin B1 and p21. Despite the up-regulation in protein expression levels by fraction F2, there was no observable expression levels of the p53 protein and p21 and p53 mRNAs under similar experimental conditions. CONCLUSION: These findings suggest that the F1 and F2 fractions of CB may provide a valuable lead for the development of novel and effective anti-neoplastic drug(s).
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Proteínas de Ciclo Celular/metabolismo
Commelina
Leucemia de Células T/metabolismo
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/uso terapêutico
Proteína Quinase CDC2
Proteínas de Ciclo Celular/genética
Proliferação Celular/efeitos dos fármacos
Ciclina B1/genética
Ciclina B1/metabolismo
Inibidor de Quinase Dependente de Ciclina p21/metabolismo
Quinases Ciclina-Dependentes/genética
Quinases Ciclina-Dependentes/metabolismo
Seres Humanos
Células Jurkat
Leucemia de Células T/tratamento farmacológico
Fitoterapia
Extratos Vegetais/uso terapêutico
Proteínas Proto-Oncogênicas c-bcl-2/genética
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
RNA Mensageiro/metabolismo
África do Sul
Linfócitos T/metabolismo
Proteína Supressora de Tumor p53/metabolismo
Regulação para Cima
Proteína X Associada a bcl-2/genética
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Cell Cycle Proteins); 0 (Cyclin B1); 0 (Cyclin-Dependent Kinase Inhibitor p21); 0 (Plant Extracts); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (RNA, Messenger); 0 (Tumor Suppressor Protein p53); 0 (bcl-2-Associated X Protein); EC 2.7.11.22 (CDC2 Protein Kinase); EC 2.7.11.22 (CDK1 protein, human); EC 2.7.11.22 (Cyclin-Dependent Kinases)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140222
[St] Status:MEDLINE
[do] DOI:10.1186/1472-6882-14-65



página 1 de 7 ir para página                  
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde