Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.238.500 [Categoria DeCS]
Referências encontradas : 406 [refinar]
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[PMID]:27771200
[Au] Autor:Kataki S; Hazarika S; Baruah DC
[Ad] Endereço:Energy Conservation Laboratory, Department of Energy, Tezpur University, Assam, India. Electronic address: sam_kat@tezu.ernet.in.
[Ti] Título:Assessment of by-products of bioenergy systems (anaerobic digestion and gasification) as potential crop nutrient.
[So] Source:Waste Manag;59:102-117, 2017 Jan.
[Is] ISSN:1879-2456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alternative fertilizer resources have drawn attention in recent times in order to cope up with ever increasing demand for fertilizer. By-products of bioenergy system are considered favourable as organic fertilizer due to their ability to recycle plant nutrients. Present study evaluates fertilizer suitability of by-products of two bioenergy systems viz. 3 types of anaerobic digestion by-products (digestate) from local surplus biomass such as cowdung, Ipomoea carnea:cowdung (60:40) and ricestraw:green gram stover:cowdung (30:30:40) and one gasification by-product (biochar) from rice husk. Digestates were assessed considering 4 different application options of each viz. whole, solid, liquid and ash from solid digestates. Digestate characteristics (organic matter, macronutrients, micronutrients and heavy metal content) were found to be a function of feedstock and processing (solid liquid separation and ashing). Ipomoea carnea based digestates in all application options showed comparatively higher N, P, K, NH -N, Ca, Mg, S and micro nutrient content than other digestates. Separation concentrated plant nutrients and organic matter in solid digestates, making these suitable both as organic amendments and fertilizer. Separated liquid digestate shared larger fraction of ammonium nitrogen (61-91% of total content), indicating their suitability as readily available N source. However, fertilizer application of liquid digestate may not match crop requirements due to lower total nutrient concentration. Higher electrical conductivity of the liquid digestates (3.4-9.3mScm ) than solid digestates (1.5-2mScm ) may impart phyto-toxic effect upon fertilization due to salinity. In case of by-products with unstable organic fraction i.e. whole and solid digestates of rice straw:green gram stover:cowdung digestates (Humification index 0.7), further processing (stabilization, composting) may be required to maximize their fertilizer benefit. Heavy metal contents of the by-products were found to be within the permitted range specified for organic fertilizer (vermicompost) in India. However, higher Al content of the digestates in whole, solid and ash phase (0.06-16.97gkg fresh matter) can be a concern in acid soil which may cause Al toxicity. Understanding on agrochemical characteristics of bioenergy by-products with varying feedstock and application option is expected to promote their valorization opportunities considering user specific requirements. In the context of agriculturally dominant but energy deficient rural Indian scenario, integrated production of bioenergy and by-product based fertilizer could be very significant to meet the critical additional requirement of both energy and fertilizer.
[Mh] Termos MeSH primário: Agricultura/métodos
Produtos Agrícolas/química
Fertilizantes/análise
Nitrogênio/análise
Eliminação de Resíduos/métodos
Esgotos/química
Solo/química
[Mh] Termos MeSH secundário: Amônia/química
Anaerobiose
Biomassa
Conservação dos Recursos Naturais
Fontes Geradoras de Energia
Gases
Concentração de Íons de Hidrogênio
Índia
Ipomoea
Metais Pesados/análise
Nitrogênio/química
Compostos Orgânicos
Resíduos Sólidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers); 0 (Gases); 0 (Metals, Heavy); 0 (Organic Chemicals); 0 (Sewage); 0 (Soil); 0 (Solid Waste); 7664-41-7 (Ammonia); N762921K75 (Nitrogen)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28890992
[Au] Autor:Chen C; Zhao T; Liu R; Luo L
[Ad] Endereço:Agricultural Clean Watershed Innovation Group, Institute of Environment and Sustainable Development in Agriculture, CAAS; Key Laboratory for Agricultural Environment MOA, Beijing, 100081, China.
[Ti] Título:Performance of five plant species in removal of nitrogen and phosphorus from an experimental phytoremediation system in the Ningxia irrigation area.
[So] Source:Environ Monit Assess;189(10):497, 2017 Sep 10.
[Is] ISSN:1573-2959
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Agricultural non-point source (ANPS) pollution is an important contributor to elevated nitrogen (N) and phosphorus (P) in surface waters, which can cause serious environmental problems. Considerable effort has therefore gone into the development of methods that control the ANPS input of N and P to surface waters. Phytoremediation has been extensively used because it is cost-effective, environmentally friendly, and efficient. The N and P loads from agricultural drainage are a potential threat to the water quality of the Yellow River in Ningxia, China. Yet, phytoremediation has only rarely been applied within the Ningxia irrigation area. In an experimental set-up, five species (Ipomoea aquatica, IA; Lactuca sativa, LS; Oryza sativa, OS; Typha latifolia, TL; Zizania latifolia, ZL) were evaluated for their ability to reduce N and P loads over 62 days and five observation periods. Total N and P concentrations, plant biomass, and nutrient content were measured. The results showed that OS, LS, and IA performed better than ZL and TL in terms of nutrients removal, biomass accumulation, and nutrients storage. The highest overall removal rates of N and P (57.7 and 57.3%, respectively) were achieved by LS treatment. In addition, plant uptake contributed significantly to nutrient removal, causing a 25.9-72.0% reduction in N removal and a 54.3-86.5% reduction in P removal. Thus, this study suggests that OS, LS, and IA would be more suitable than ZL and TL for controlling nutrient loads in the Ningxia irrigation area using phytoremediation.
[Mh] Termos MeSH primário: Nitrogênio/metabolismo
Fósforo/metabolismo
Plantas/metabolismo
Poluição da Água/prevenção & controle
[Mh] Termos MeSH secundário: Irrigação Agrícola/normas
Biodegradação Ambiental
Biomassa
China
Monitoramento Ambiental
Eutrofização
Ipomoea/metabolismo
Alface/metabolismo
Nitrogênio/análise
Oryza/metabolismo
Fósforo/análise
Plantas/classificação
Poaceae/metabolismo
Distribuição Aleatória
Rios
Typhaceae/metabolismo
Qualidade da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
27YLU75U4W (Phosphorus); N762921K75 (Nitrogen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE
[do] DOI:10.1007/s10661-017-6213-y


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[PMID]:28571312
[Au] Autor:Lee ST; Gardner DR; Cook D
[Ad] Endereço:Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture , 1150 East, 1400 North, Logan, Utah 84341, United States.
[Ti] Título:Identification of Indole Diterpenes in Ipomoea asarifolia and Ipomoea muelleri, Plants Tremorgenic to Livestock.
[So] Source:J Agric Food Chem;65(26):5266-5277, 2017 Jul 05.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ipomoea asarifolia has been associated with a tremorgenic syndrome in livestock in Brazil and was recently reported to contain tremorgenic indole diterpenes. Ipomoea muelleri has been reported to cause a similar tremorgenic syndrome in livestock in Australia. Ipomoea asarifolia and I. muelleri were investigated by high-performance liquid chromatography-high-resolution mass spectometry (HPLC-HRMS) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for indole diterpene composition. The high-resolution mass spectrometric data in combination with MS/MS fragmentation mass spectral data provided valuable information for indole diterpene characterization. The previous report of indole diterpenes in I. asarifolia was confirmed and expanded; and the presence of indole diterpenes in I. muelleri is reported for the first time. Two new indole diterpenes were isolated and their structures determined by 1D and 2D NMR spectroscopy and given the names 11-hydroxy-12,13-epoxyterpendole K and 6,7-dehydroterpendole A. The presence of terpendole K and terpendole E in I. asarifolia is unequivocally demonstrated for the first time. This is the first detailed MS analysis of known indole diterpenes and possible isomers in I. asarifolia and I. muelleri.
[Mh] Termos MeSH primário: Diterpenos/química
Indóis/química
Ipomoea/química
Extratos Vegetais/química
Tremor/veterinária
[Mh] Termos MeSH secundário: Animais
Diterpenos/toxicidade
Indóis/toxicidade
Ipomoea/toxicidade
Gado
Espectroscopia de Ressonância Magnética
Espectrometria de Massas
Estrutura Molecular
Extratos Vegetais/toxicidade
Tremor/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diterpenes); 0 (Indoles); 0 (Plant Extracts)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01834


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[PMID]:28478100
[Au] Autor:Dewanjee S; Joardar S; Bhattacharjee N; Dua TK; Das S; Kalita J; Manna P
[Ad] Endereço:Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India. Electronic address: s.dewanjee@yahoo.com.
[Ti] Título:Edible leaf extract of Ipomoea aquatica Forssk. (Convolvulaceae) attenuates doxorubicin-induced liver injury via inhibiting oxidative impairment, MAPK activation and intrinsic pathway of apoptosis.
[So] Source:Food Chem Toxicol;105:322-336, 2017 Jul.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ipomoea aquatica Forssk. (Convolvulaceae) is an aquatic vegetable traditionally employed against toxic effects of xenobiotics. The present study has been designed to investigate the molecular mechanism underlying the beneficial role of the edible (aqueous) leaf extract of I. aquatica (AEIA) against doxorubicin (Dox)-induced liver injury. AEIA exhibited a dose-dependent (∼400 µg/ml) increase in cell viability against Dox (1 µM) in isolated rodent hepatocytes. AEIA (400 µg/ml) prevented the Dox-induced increase in ROS, redox imbalance, and activation of mitogen activated protein kinases (MAPK) and intrinsic pathway of apoptosis in hepatocytes. In the in vivo assay, administration of AEIA (100 mg/kg, p.o.) against Dox (3 mg/kg, i.p.) also reduced the oxidative impairment, DNA fragmentation, ATP formation, and up-regulated the mitochondrial co-enzymes Qs in the liver tissues of Wistar rats. Histological assessments were in agreement with the biochemical findings. Substantial quantities of phyto-antioxidants in AEIA may mediate its beneficial function against Dox-induced liver injury.
[Mh] Termos MeSH primário: Antineoplásicos/efeitos adversos
Apoptose/efeitos dos fármacos
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Doxorrubicina/efeitos adversos
Ipomoea/química
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Extratos Vegetais/administração & dosagem
Folhas de Planta/química
[Mh] Termos MeSH secundário: Animais
Doença Hepática Induzida por Substâncias e Drogas/etiologia
Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Seres Humanos
Fígado/efeitos dos fármacos
Fígado/lesões
Fígado/metabolismo
Masculino
Estresse Oxidativo/efeitos dos fármacos
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Extracts); 80168379AG (Doxorubicin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170508
[St] Status:MEDLINE


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[PMID]:28476977
[Au] Autor:Das M; Mondal A; Patowary K; Malipeddi H
[Ad] Endereço:Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632 014, Tamil Nadu, India.
[Ti] Título:Biosynthesis of AgNPs using aqueous leaf extract of and their anti-inflammatory effect on carrageenan-induced paw edema in male Wistar rats.
[So] Source:IET Nanobiotechnol;11(3):225-229, 2017 Apr.
[Is] ISSN:1751-8741
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Silver nanoparticles (AgNPs) were synthesised from aqueous Ag nitrate through a simple, competent and eco-friendly method using the leaf extract of as reducing as well as capping agent. Ultraviolet-visible absorption spectroscopy was used to confirm the formation of AgNPs which displayed the substantiation of surface plasmon bands at 425 nm. The NPs were also characterised using Fourier transformer infrared spectroscopy, X-ray diffraction method, transmission electron microscope and zeta potential. The characterisation study confirmed the formation of AgNPs, their spherical shape and average diameter of 12.85 ± 8.65 nm. Zeta potential value of -20.5 mV suggested that the AgNPs are stable in the suspension. The aqueous extract and the AgNPs were further screened for in vivo anti-inflammatory activity using carrageenan-induced paw edema in male Wistar rats. The study demonstrated that the AgNPs (1 ml kg ) had a significant ( < 0.05) anti-edemic effect and inhibition was observed from the first hour (21.31 ± 1.34) until the sixth hour (52.67 ± 1.41), when the inhibitory effect was greatest and superior to the aqueous extract and the standard, diclofenac.
[Mh] Termos MeSH primário: Edema/tratamento farmacológico
Ipomoea/química
Nanopartículas Metálicas/administração & dosagem
Extratos Vegetais/química
Folhas de Planta/química
Prata/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/administração & dosagem
Anti-Inflamatórios/química
Produtos Biológicos/administração & dosagem
Produtos Biológicos/química
Chondrus
Relação Dose-Resposta a Droga
Edema/induzido quimicamente
Edema/patologia
Química Verde/métodos
Masculino
Nanopartículas Metálicas/química
Extratos Vegetais/administração & dosagem
Ratos
Ratos Wistar
Prata/química
Resultado do Tratamento
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Biological Products); 0 (Plant Extracts); 059QF0KO0R (Water); 3M4G523W1G (Silver)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170507
[St] Status:MEDLINE
[do] DOI:10.1049/iet-nbt.2016.0034


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[PMID]:28472155
[Au] Autor:Lin YC; Chang SC; Juang RH
[Ad] Endereço:Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan.
[Ti] Título:Plastidial α-glucan phosphorylase 1 complexes with disproportionating enzyme 1 in Ipomoea batatas storage roots for elevating malto-oligosaccharide metabolism.
[So] Source:PLoS One;12(5):e0177115, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:It has been proposed that malto-oligosaccharides (MOSs) are possibly recycled back into amylopectin biosynthesis via the sequential reactions catalyzed by plastidial α-glucan phosphorylase 1 (Pho1) and disproportionating enzyme 1 (Dpe1). In the present study, the reciprocal co-immunoprecipitation experiments using specific antibodies against Pho1 and Dpe1 demonstrated that these two enzymes can form a complex (the PD complex) in Ipomoea batatas storage roots. The immunohistochemistry analyses also revealed the co-localization of Pho1 and Dpe1 in the amyloplasts, and the protein levels of Pho1 and Dpe1 increased gradually throughout sweet potato storage root development. A high molecular weight PD complex was co-purified from sweet potato storage root lysates by size exclusion chromatography. Enzyme kinetic analyses showed that the PD complex can catalyze maltotriose and maltotetraose to generate glucose-1-phosphate in the presence of inorganic phosphate, and it also performs greater Dpe1 activity toward MOSs than does free form Dpe1. These data suggest that Pho1 and Dpe1 may form a metabolon complex, which provides elevated metabolic fluxes for MOS metabolism via a direct transfer of sugar intermediates, resulting in recycling of glucosyl units back into amylopectin biosynthesis more efficiently.
[Mh] Termos MeSH primário: Ipomoea/enzimologia
Oligossacarídeos/metabolismo
Fosforilases/metabolismo
Raízes de Plantas/enzimologia
Plastídeos/enzimologia
[Mh] Termos MeSH secundário: Catálise
Cromatografia em Gel
Imunoprecipitação
Ipomoea/metabolismo
Espectrometria de Massas
Raízes de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligosaccharides); EC 2.4.1.- (Phosphorylases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177115


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[PMID]:28287471
[Au] Autor:Yu BW; Sun JJ; Pan JT; Wu XH; Yin YQ; Yan YS; Hu JY
[Ad] Endereço:School of Traditional Chinese Medicinal Chemistry, Guangdong Pharmaceutical University, Guangzhou 510006, China. bondbeth@126.com.
[Ti] Título:Four Pentasaccharide Resin Glycosides from Argyreia acuta.
[So] Source:Molecules;22(3), 2017 Mar 11.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Four pentasaccharide resin glycosides, acutacoside F-I ( - ), were isolated from the aerial parts of . These compounds were characterized as a group of macrolactones of operculinic acid A, and their lactonization site of 11 -hydroxyhexadecanoic acid was esterified at the second saccharide moiety (Rhamnose) at C-2. The absolute configuration of the aglycone was . Their structures were elucidated by established spectroscopic and chemical methods.
[Mh] Termos MeSH primário: Glicosídeos/química
Ipomoea/química
Lactonas/química
Oligossacarídeos/química
Resinas Vegetais/química
[Mh] Termos MeSH secundário: Glicosídeos/isolamento & purificação
Lactonas/isolamento & purificação
Estrutura Molecular
Oligossacarídeos/isolamento & purificação
Ácidos Palmíticos/química
Componentes Aéreos da Planta/química
Extratos Vegetais/química
Ramnose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycosides); 0 (Lactones); 0 (Oligosaccharides); 0 (Palmitic Acids); 0 (Plant Extracts); 0 (Resins, Plant); 0 (operculinic acid); QN34XC755A (Rhamnose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE


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[PMID]:28249191
[Au] Autor:Su S; Zeng X; Bai L; Williams PN; Wang Y; Zhang L; Wu C
[Ad] Endereço:Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture, Beijing, PR China. Electronic address: sushiming@caas.cn.
[Ti] Título:Inoculating chlamydospores of Trichoderma asperellum SM-12F1 changes arsenic availability and enzyme activity in soils and improves water spinach growth.
[So] Source:Chemosphere;175:497-504, 2017 May.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arsenic (As)-contaminated agricultural soils threaten crop yields and pose a human health risk. Augmentation of exogenous microorganisms exhibiting plant-growth promoting and As speciation changing shows potential to improve crop growth and change soil As availability. Trichoderma asperellum SM-12F1 exhibiting both traits was developed into chlamydospores to improve its persistence in contaminated soils. After inoculation, As availability and enzyme activity in two types of soils and the growth as well as As uptake of water spinach (Ipomoea aquatic Forsk.) were investigated. The results indicated that inoculation significantly improved water spinach growth in both soils. Inoculating chlamydospores at 5% significantly increased As concentration (139%), bioconcentration factor (150%), and translocation factor (150%) in water spinach grown in Chenzhou (CZ) soils, while no significant change for these in Shimen (SM) soils. Inoculating chlamydospores at 5% caused a significant increase (16%) of available As content in CZ soils, while a significant decrease (13%) in SM soils. Inoculation significantly caused As methylation in both soils, while significant As reduction merely observed in CZ soils. The differential changes in available As contents in both soils were attributed to the soil pH, As fractionations and speciation characteristics. Furthermore, Inoculating chlamydospores at 5% significantly improved the activities of ß-glucosidase (155%), chitinase (211%), and phosphatase (108%) in SM soils, while significant decreases in ß-glucosidase (81%), phosphatase (54%), aminopeptidase (60%), and catalase (67%) in CZ soils. Bioaugmentation and As availability change were responsible for this result. These observations will be helpful for the application of fungal chlamydospores in the future bioremediation.
[Mh] Termos MeSH primário: Arsênico/análise
Ipomoea/crescimento & desenvolvimento
Poluentes do Solo/análise
Solo/normas
Trichoderma/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Agricultura
Arsênico/metabolismo
Biodegradação Ambiental
Seres Humanos
Ipomoea/metabolismo
Metilação
Solo/química
Poluentes do Solo/metabolismo
Esporos Fúngicos/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 0 (Soil Pollutants); N712M78A8G (Arsenic)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE


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[PMID]:28086804
[Au] Autor:Ponniah SK; Thimmapuram J; Bhide K; Kalavacharla VK; Manoharan M
[Ad] Endereço:Department of Agriculture, University of Arkansas at Pine Bluff, Pine Bluff, Arkansas, USA.
[Ti] Título:Comparative analysis of the root transcriptomes of cultivated sweetpotato (Ipomoea batatas [L.] Lam) and its wild ancestor (Ipomoea trifida [Kunth] G. Don).
[So] Source:BMC Plant Biol;17(1):9, 2017 Jan 13.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The complex process of formation of storage roots (SRs) from adventitious roots affects sweetpotato yield. Identifying the genes that are uniquely expressed in the SR forming cultivated species, Ipomoea batatas (Ib), and its immediate ancestral species, Ipomoea trifida (It), which does not form SRs, may provide insights into the molecular mechanisms underlying SR formation in sweetpotato. RESULTS: Illumina paired-end sequencing generated ~208 and ~200 million reads for Ib and It, respectively. Trinity assembly of the reads resulted in 98,317 transcripts for Ib and 275,044 for It, after post-assembly removal of trans-chimeras. From these sequences, we identified 4,865 orthologous genes in both Ib and It, 60 paralogous genes in Ib and 2,286 paralogous genes in It. Among paralogous gene sets, transcripts encoding the transcription factor RKD, which may have a role in nitrogen regulation and starch formation, and rhamnogalacturonate lyase (RGL) family proteins, which produce the precursors of cell wall polysaccharides, were found only in Ib. In addition, transcripts encoding a K efflux antiporter (KEA5) and the ERECTA protein kinase, which function in phytohormonal regulation and root proliferation, respectively, were also found only in Ib. qRT-PCR indicated that starch and sucrose metabolism genes, such as those encoding ADP-glucose pyrophosphorylase and beta-amylase, showed lower expression in It than Ib, whereas lignin genes such as caffeoyl-CoA O-methyltransferase (CoMT) and cinnamyl alcohol dehydrogenase (CAD) showed higher expression in It than Ib. A total of 7,067 and 9,650 unique microsatellite markers, 1,037,396 and 495,931 single nucleotide polymorphisms (SNPs) and 103,439 and 69,194 InDels in Ib and It, respectively, were also identified from this study. CONCLUSION: The detection of genes involved in the biosynthesis of RGL family proteins, the transcription factor RKD, and genes encoding a K efflux antiporter (KEA5) and the ERECTA protein kinase only in I. batatas indicate that these genes may have important functions in SR formation in sweetpotato. Potential molecular markers (SNPs, simple sequence repeats and InDels) and sequences identified in this study may represent a valuable resource for sweetpotato gene annotation and may serve as important tools for improving SR formation in sweetpotato through breeding.
[Mh] Termos MeSH primário: Ipomoea batatas/genética
Ipomoea/genética
Raízes de Plantas/genética
[Mh] Termos MeSH secundário: DNA de Plantas
Mutagênese Insercional
Polimorfismo de Nucleotídeo Único
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de DNA
Deleção de Sequência
Transcriptoma
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170115
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-016-0950-x


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[PMID]:27992771
[Au] Autor:Shen C; Huang YY; He CT; Zhou Q; Chen JX; Tan X; Mubeen S; Yuan JG; Yang ZY
[Ad] Endereço:State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Xingang Xi Road 135, Guangzhou, 510275, China.
[Ti] Título:Comparative analysis of cadmium responsive microRNAs in roots of two Ipomoea aquatica Forsk. cultivars with different cadmium accumulation capacities.
[So] Source:Plant Physiol Biochem;111:329-339, 2017 Feb.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:In plants, microRNAs (miRNAs) play regulatory roles in response to various environmental stresses. In order to illustrate the regulation mechanisms of miRNAs involving the different Cd accumulation abilities between a low-shoot-Cd cultivar (QLQ) and a high-shoot-Cd cultivar (T308) of water spinach (Ipomoea aquatic Forsk.), six sRNA libraries at 3 different time points were constructed. Only 5 miRNAs were exclusively regulated in QLQ, among them, miRNA395 was up-regulated, which was supposed to enhance the Cd retention and detoxification in root. Also, the alterations of miRNA5139, miRNA1511 and miRNA8155 contributed to the attenuation of Cd translocation into the shoot of QLQ. More differentially expressed miRNAs were observed in T308, indicating more complex response was adopted by T308 under Cd stress. miRNA397 exclusively regulated in T308 has enhanced the Cd influx of T308 under Cd treatments. Besides, the Cd translocation of T308 was strengthened due to the up-regulation of MATE efflux family, which was targeted by miRNA3627. Our results unraveled the effects of the cultivar-dependent expression of these specific miRNAs on the different Cd accumulation and translocation abilities of QLQ and T308. These findings provide a new perspective for the molecular assisted breeding of low-Cd cultivars for leaf-vegetables.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Ipomoea/genética
MicroRNAs/genética
Raízes de Plantas/genética
[Mh] Termos MeSH secundário: Análise por Conglomerados
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Ontologia Genética
Ipomoea/efeitos dos fármacos
MicroRNAs/metabolismo
Raízes de Plantas/efeitos dos fármacos
Brotos de Planta/efeitos dos fármacos
Brotos de Planta/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA de Plantas/genética
RNA de Plantas/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Análise de Sequência de RNA
Spinacia oleracea/metabolismo
Água/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (RNA, Messenger); 0 (RNA, Plant); 00BH33GNGH (Cadmium); 059QF0KO0R (Water)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161220
[St] Status:MEDLINE



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