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  1 / 1019 MEDLINE  
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[PMID]:29218572
[Au] Autor:Peng X; Pan H; Muhammad A; An H; Fang S; Li W; Zhang S
[Ad] Endereço:Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025, Hubei, China.
[Ti] Título:Complete genome sequence of a new strain of Lagenaria siceraria endornavirus from China.
[So] Source:Arch Virol;163(3):805-808, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:An RNA virus tentatively named Lagenaria siceraria endornavirus-Hubei (LsEV-HuB) was isolated from Lagenaria siceraria var. hispida in Hubei, China. The LsEV-HuB genome consists of 15,098 bp and contains a single open reading frame (ORF) encoding a large protein with several conserved domains, including one helicase domain, one glycosyltransferase domain, two capsular polysaccharide synthesis protein (CPS) domains, and one RNA-dependent RNA polymerase (RdRp) domain. LsEV-HuB has nucleotide and amino acid sequence identities of 72.96% and 77.95%, respectively, to Lagenaria siceraria endornavirus-California (LsEV-CA), the closest relative of LsEV-HuB.
[Mh] Termos MeSH primário: Cucurbitaceae/virologia
DNA Viral/genética
Genoma Viral
Filogenia
Poliproteínas/genética
Vírus de RNA/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
China
Tamanho do Genoma
Fases de Leitura Aberta
Doenças das Plantas/virologia
Vírus de RNA/classificação
Vírus de RNA/isolamento & purificação
Recombinação Genética
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Sequenciamento Completo do Genoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Polyproteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3664-y


  2 / 1019 MEDLINE  
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[PMID]:29267293
[Au] Autor:Liu S; Xing Z; Wang Z; Tian S; Jahun FR
[Ad] Endereço:College of Engineering, Shenyang Agricultural University, Shenyang, China.
[Ti] Título:Development of machine-vision system for gap inspection of muskmelon grafted seedlings.
[So] Source:PLoS One;12(12):e0189732, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Grafting robots have been developed in the world, but some auxiliary works such as gap-inspecting for grafted seedlings still need to be done by human. An machine-vision system of gap inspection for grafted muskmelon seedlings was developed in this study. The image acquiring system consists of a CCD camera, a lens and a front white lighting source. The image of inspected gap was processed and analyzed by software of HALCON 12.0. The recognition algorithm for the system is based on principle of deformable template matching. A template should be created from an image of qualified grafted seedling gap. Then the gap image of the grafted seedling will be compared with the created template to determine their matching degree. Based on the similarity between the gap image of grafted seedling and the template, the matching degree will be 0 to 1. The less similar for the grafted seedling gap with the template the smaller of matching degree. Thirdly, the gap will be output as qualified or unqualified. If the matching degree of grafted seedling gap and the template is less than 0.58, or there is no match is found, the gap will be judged as unqualified; otherwise the gap will be qualified. Finally, 100 muskmelon seedlings were grafted and inspected to test the gap inspection system. Results showed that the gap inspection machine-vision system could recognize the gap qualification correctly as 98% of human vision. And the inspection speed of this system can reach 15 seedlings·min-1. The gap inspection process in grafting can be fully automated with this developed machine-vision system, and the gap inspection system will be a key step of a fully-automatic grafting robots.
[Mh] Termos MeSH primário: Cucurbitaceae
Fotografia/instrumentação
Plântulas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189732


  3 / 1019 MEDLINE  
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[PMID]:28952732
[Au] Autor:Mong SK; Cochran FV; Yu H; Graziano Z; Lin YS; Cochran JR; Pentelute BL
[Ad] Endereço:Department of Chemistry, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
[Ti] Título:Heterochiral Knottin Protein: Folding and Solution Structure.
[So] Source:Biochemistry;56(43):5720-5725, 2017 Oct 31.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Homochirality is a general feature of biological macromolecules, and Nature includes few examples of heterochiral proteins. Herein, we report on the design, chemical synthesis, and structural characterization of heterochiral proteins possessing loops of amino acids of chirality opposite to that of the rest of a protein scaffold. Using the protein Ecballium elaterium trypsin inhibitor II, we discover that selective ß-alanine substitution favors the efficient folding of our heterochiral constructs. Solution nuclear magnetic resonance spectroscopy of one such heterochiral protein reveals a homogeneous global fold. Additionally, steered molecular dynamics simulation indicate ß-alanine reduces the free energy required to fold the protein. We also find these heterochiral proteins to be more resistant to proteolysis than homochiral l-proteins. This work informs the design of heterochiral protein architectures containing stretches of both d- and l-amino acids.
[Mh] Termos MeSH primário: Cucurbitaceae/química
Simulação de Dinâmica Molecular
Proteínas de Plantas/química
Dobramento de Proteína
[Mh] Termos MeSH secundário: Cucurbitaceae/genética
Proteínas de Plantas/genética
Domínios Proteicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (trypsin inhibitor EETI II protein, plant)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00722


  4 / 1019 MEDLINE  
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[PMID]:28887587
[Au] Autor:Ruangnam S; Wanchana S; Phoka N; Saeansuk C; Mahatheeranont S; de Hoop SJ; Toojinda T; Vanavichit A; Arikit S
[Ad] Endereço:Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand.
[Ti] Título:A deletion of the gene encoding amino aldehyde dehydrogenase enhances the "pandan-like" aroma of winter melon (Benincasa hispida) and is a functional marker for the development of the aroma.
[So] Source:Theor Appl Genet;130(12):2557-2565, 2017 Dec.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: The gene conferring a "pandan-like" aroma of winter melon was identified. The sequence variation (804-bp deletion) found in the gene was used as the target for functional marker development. Winter melon (Benincasa hispida), a member of the Cucurbitaceae family, is a commonly consumed vegetable in Asian countries that is popular for its nutritional and medicinal value. A "pandan-like" aroma, which is economically important in crops including rice and soybean, is rarely found in most commercial varieties of winter melon, but is present in some landraces. This aroma is a value-added potential trait in breeding winter melon with a higher economic value. In this study, we confirmed that the aroma of winter melon is due to the potent volatile compound 2-acetyl-1-pyrroline (2AP) as previously identified in other plants. Based on an analysis of public transcriptome data, BhAMADH encoding an aminoaldehyde dehydrogenase (AMADH) was identified as a candidate gene conferring aroma of winter melon. A sequence comparison of BhAMADH between the aromatic and non-aromatic accessions revealed an 804-bp deletion encompassing exons 11-13 in the aromatic accession. The deletion caused several premature stop codons and could result in a truncated protein with a length of only 208 amino acids compared with 503 amino acids in the normal protein. A functional marker was successfully developed based on the 804-bp deletion and validated in 237 F progenies. A perfect association of the marker genotypes and aroma phenotypes indicates that BhAMADH is the major gene conferring the aroma. The recently developed functional marker could be efficiently used in breeding programs for the aroma trait in winter melon.
[Mh] Termos MeSH primário: Aldeído Desidrogenase/genética
Cucurbitaceae/genética
Odorantes
Pirróis/química
Deleção de Sequência
[Mh] Termos MeSH secundário: Produtos Agrícolas/enzimologia
Produtos Agrícolas/genética
Cucurbitaceae/enzimologia
Genes de Plantas
Marcadores Genéticos
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Pyrroles); EC 1.2.1.3 (Aldehyde Dehydrogenase); IGC0W6LY94 (2-acetyl-1-pyrroline)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170910
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2976-3


  5 / 1019 MEDLINE  
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[PMID]:28825206
[Au] Autor:Costa TM; Blawid R; da Costa Junior AC; Lima MF; de Aragão FAS; de Andrade GP; Pio-Ribeiro G; Aranda MA; Inoue-Nagata AK; Nagata T
[Ad] Endereço:Departmento de Biologia Celular, Universidade de Brasília, Brasília, DF, 70910-900, Brazil.
[Ti] Título:Complete genome sequence of melon yellowing-associated virus from melon plants with the severe yellowing disease in Brazil.
[So] Source:Arch Virol;162(12):3899-3901, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Here, we describe the complete genome sequence of melon yellowing-associated virus (MYaV), found in melon plants with severe yellowing disease, determined by high-throughput and Sanger sequencing. MYaV has an RNA genome of 9073 nucleotides plus a poly(A) tail. At least six open reading frames were predicted, with a typical carlavirus genomic organisation. Phylogenetic analysis of the complete genome sequence and the amino acid sequences of the RNA-dependent RNA polymerase confirmed that MYaV belongs to the genus Carlavirus, with the highest genome-wide nucleotide sequence identity of 59.8% to sweet potato yellow mottle virus.
[Mh] Termos MeSH primário: Carlavirus/classificação
Carlavirus/isolamento & purificação
Cucurbitaceae/virologia
Genoma Viral
Doenças das Plantas/virologia
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Brasil
Carlavirus/genética
Fases de Leitura Aberta
Filogenia
RNA Viral/genética
Vírus Satélites
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3532-9


  6 / 1019 MEDLINE  
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[PMID]:28641205
[Au] Autor:Abbas M; Arshad M; Nisar N; Nisar J; Ghaffar A; Nazir A; Asif Tahir M; Iqbal M
[Ad] Endereço:Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan; Jhang-Campus, University of Veterinary & Animal Sciences, Lahore, Pakistan. Electronic address: mazhar.abbas@uvas.edu.pk.
[Ti] Título:Muscilage characterization, biochemical and enzymatic activities of laser irradiated Lagenaria siceraria seedlings.
[So] Source:J Photochem Photobiol B;173:344-352, 2017 Aug.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Laser stimulation effect on L. siceraria seed mucilage, biochemicals and enzymatic activities during early growth stages were investigated. The laser density power of 1mW/cm for 3 and 5min treatments were performed and various responses i.e., seedlings mucilage, biochemical and enzymatic activities were studied. Laser treatment of L. siceraria seeds enhanced the biochemical as well as the enzymatic activities. TPC (total phenolic contents),TFC (total flavonoids contents), TSS (total soluble sugar), reducing sugar, proline contents, total soluble protein and nitrogen contents were recorded higher in laser treated groups versus control. Mucilage from L. siceraria seed coat was also characterized. The pre-sowing seeds were treated with laser radiation for 3 and 5min. TPC, TFC, proline contents, total soluble protein and nitrogenous compounds contents, ascorbic acid contents were recorded higher at 3min. The laser irradiation effect on TSS, hydrogen peroxide (H O ), malondialdehyde (MDA) was insignificant versus control. The SOD (superoxide dismutase) and POD (peroxidase), AMY (amylase), CAT (catalase) activities were recorded higher for 5min laser treatment. Results revealed that He-Ne continuous wave-laser pre-sowing seed irradiation affected the seed coat mucilage, biochemical and enzymatic activities positively and this treatment could possibly be used to enhance the L. siceraria productivity. Future study will be focused on growth at later stages and yield characteristics of L. siceraria.
[Mh] Termos MeSH primário: Cucurbitaceae/enzimologia
Cucurbitaceae/efeitos da radiação
Lasers
Plântulas/enzimologia
Plântulas/efeitos da radiação
[Mh] Termos MeSH secundário: Cucurbitaceae/crescimento & desenvolvimento
Cucurbitaceae/metabolismo
Proteínas de Plantas/metabolismo
Plântulas/crescimento & desenvolvimento
Plântulas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE


  7 / 1019 MEDLINE  
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[PMID]:28625732
[Au] Autor:Li P; Zhu N; Hu M; Wu H; Yu T; Wu T; Zhang D; Sun Z; Yang J; Ma G; Xu X
[Ad] Endereço:Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100193, People's Republic of China.
[Ti] Título:New cucurbitane triterpenoids with cytotoxic activities from Hemsleya penxianensis.
[So] Source:Fitoterapia;120:158-163, 2017 Jul.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Seven new cucurbitane triterpenoids, Pengxianencins A-G (1-7) including one alkaloid, were isolated from the ethanol extract of the tubers of Hemsleya penxianensis. Their structures were elucidated on the basis of extensive spectroscopic data, including 1D and 2D NMR spectra as well as HR-ESI-MS. The evaluation of inhibition activity against human Hela, MCF-7, and A-549 cell lines showed that compounds 1, 4, 6, 7 have different levels of cytotoxic activities, with IC values ranging from 1.67 to 45.28µM.
[Mh] Termos MeSH primário: Cucurbitaceae/química
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Células A549
Células HeLa
Seres Humanos
Células MCF-7
Estrutura Molecular
Extratos Vegetais/química
Tubérculos/química
Triterpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Triterpenes)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


  8 / 1019 MEDLINE  
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[PMID]:28592995
[Au] Autor:Latrasse D; Rodriguez-Granados NY; Veluchamy A; Mariappan KG; Bevilacqua C; Crapart N; Camps C; Sommard V; Raynaud C; Dogimont C; Boualem A; Benhamed M; Bendahmane A
[Ad] Endereço:Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, University Paris-Sud, University of Evry, University Paris-Diderot, Sorbonne Paris-Cite, University of Paris-Saclay, Batiment 630, 91405 Orsay, France.
[Ti] Título:The quest for epigenetic regulation underlying unisexual flower development in .
[So] Source:Epigenetics Chromatin;10:22, 2017.
[Is] ISSN:1756-8935
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Melon ( ) is an important vegetable crop from the family and a reference model specie for sex determination, fruit ripening and vascular fluxes studies. Nevertheless, the nature and role of its epigenome in gene expression regulation and more specifically in sex determination remains largely unknown. RESULTS: We have investigated genome wide H3K27me3 and H3K9ac histone modifications and gene expression dynamics, in five melon organs. H3K9ac and H3K27me3 were mainly distributed along gene-rich regions and constrained to gene bodies. H3K9ac was preferentially located at the TSS, whereas H3K27me3 distributed uniformly from TSS to TES. As observed in other species, H3K9ac and H3K27me3 correlated with high and low gene expression levels, respectively. Comparative analyses of unisexual flowers pointed out sex-specific epigenetic states of TFs involved in ethylene response and flower development. Chip-qPCR analysis of laser dissected carpel and stamina primordia, revealed sex-specific histone modification of MADS-box genes. Using sex transition mutants, we demonstrated that the female promoting gene, , represses the expression of the male promoting gene via deposition of H3K27me3. CONCLUSIONS: Our findings reveal the organ-specific landscapes of H3K9ac and H3K27me3 in melon. Our results also provide evidence that the sex determination genes recruit histone modifiers to orchestrate unisexual flower development in monoecious species.
[Mh] Termos MeSH primário: Cucurbitaceae/genética
Epigênese Genética
Flores/genética
Processos de Determinação Sexual
[Mh] Termos MeSH secundário: Cucurbitaceae/crescimento & desenvolvimento
Flores/crescimento & desenvolvimento
Regulação da Expressão Gênica de Plantas
Genoma de Planta
Histonas
Proteínas de Plantas/genética
Reprodução/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histones); 0 (Plant Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1186/s13072-017-0132-6


  9 / 1019 MEDLINE  
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[PMID]:28578021
[Au] Autor:Mohanty JN; Nayak S; Jha S; Joshi RK
[Ad] Endereço:Functional Genomics Laboratory, Centre of Biotechnology, Siksha O Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar 751003, India.
[Ti] Título:Transcriptome profiling of the floral buds and discovery of genes related to sex-differentiation in the dioecious cucurbit Coccinia grandis (L.) Voigt.
[So] Source:Gene;626:395-406, 2017 Aug 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Dioecious species offer an inclusive structure to study the molecular basis of sexual dimorphism in angiosperms. Despite having a small genome and heteromorphic sex chromosomes, Coccinia grandis is a highly neglected dioecious species with little information available on its physical state, genetic orientation and key sex-defining elements. In the present study, we performed RNA-Seq and DGE analysis of male (MB) and female (FB) buds in C. grandis to gain insights into the molecular basis of sex determination in this plant. De novo assembly of 75 million clean reads resulted in 72,479 unigenes for male library and 63,308 unigenes for female library with a mean length of 736bp. 61,458 (85.57%) unigenes displayed significant similarity with protein sequences from publicly available databases. Comparative transcriptome analyses revealed 1410 unigenes as differentially expressed (DEGs) between MB and FB samples. A consistent correlation between the expression levels of DEGs was observed for the RNA-Seq pattern and qRT-PCR validation. Functional annotation showed high enrichment of DEGs involved in phytohormone biosynthesis, hormone signaling and transduction, transcriptional regulation and methyltransferase activity. High induction of hormone responsive genes such as ARF6, ACC synthase1, SNRK2 and BRI1-associated receptor kinase 1 (BAK1) suggest that multiple phytohormones and their signaling crosstalk play crucial role in sex determination in this species. Beside, the transcription factors such as zinc fingers, homeodomain leucine zippers and MYBs were identified as major determinants of male specific expression. Moreover, the detection of multiple DEGs as the miRNA target site implies that a small RNA mediated gene silencing cascade may also be regulating gender differentiation in C. grandis. Overall, the present transcriptome resources provide us a large number of DEGs involved in sex expression and could form the groundwork for unravelling the molecular mechanism of sex determination in C. grandis.
[Mh] Termos MeSH primário: Cucurbitaceae/genética
Flores/genética
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Transcriptoma
[Mh] Termos MeSH secundário: Cromossomos de Plantas/genética
Cucurbitaceae/crescimento & desenvolvimento
Flores/crescimento & desenvolvimento
MicroRNAs/genética
Organogênese Vegetal/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Cromossomos Sexuais/genética
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170605
[St] Status:MEDLINE


  10 / 1019 MEDLINE  
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[PMID]:28573224
[Au] Autor:Wang WD; Liu Y; Su Y; Xiong XZ; Shang D; Xu JJ; Liu HJ
[Ad] Endereço:Department of Thoracic surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
[Ti] Título:ANTITUMOR AND APOPTOTIC EFFECTS OF CUCURBITACIN A IN A-549 LUNG CARCINOMA CELLS IS MEDIATED VIA G2/M CELL CYCLE ARREST AND M-TOR/PI3K/AKT SIGNALLING PATHWAY.
[So] Source:Afr J Tradit Complement Altern Med;14(2):75-82, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The main aim of this study was to demonstrate the antitumor potential of cucurbitacin A on A-549 NSCLC (non-small cell lung cancer cells). The effects of Cucurbitacin A on apoptotic induction, cell physic, cell cycle failure and m-TOR/PI3K/Akt signalling pathway were also investigated in the present study. MATERIALS AND METHODS: MTT assay and clonogenic assay were carried out to study effects of this compound on cell cytotoxicity and colony forming tendency in A-549 cells. Moreover, phase and fluorescence microscopic techniques were used to examine the effects on cell morphology and induction of apoptosis. The effects on cell cycle phase distribution were investigated by flow cytometry and effects on m-TOR/PI3K/Akt signalling proteins were assessed by western blot analysis. RESULTS: Results showed that cucurbitacin A induced dose-dependent cytotoxic effects along with suppressing the colony forming tendency in these cells. Cucurbitacin A also induced morphological changes in these cells featuring chromatin condensation, cell shrinkage and apoptotic body formation. G2/M phase cell cycle collapse was also induced by Cucurbitacin A along with inhibition of expression levels of m-TOR/PI3K/Akt proteins. CONCLUSIONS: In conclusion, cucurbitacin A inhibits cancer growth in A-549 NSCLC cells by inducing apoptosis, targeting m-TOR/PI3K/Akt signalling pathway and G2/M cell cycle.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/uso terapêutico
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Cucurbitacinas/uso terapêutico
Neoplasias Pulmonares/tratamento farmacológico
Pulmão/efeitos dos fármacos
Fosfotransferases/metabolismo
Fitoterapia
[Mh] Termos MeSH secundário: Células A549
Antineoplásicos Fitogênicos/farmacologia
Apoptose
Carcinoma/tratamento farmacológico
Carcinoma/metabolismo
Cucurbitaceae/química
Cucurbitacinas/farmacologia
Seres Humanos
Neoplasias Pulmonares/metabolismo
Neoplasias Pulmonares/patologia
Fosfatidilinositol 3-Quinases/metabolismo
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
Proteínas Proto-Oncogênicas c-akt/metabolismo
Transdução de Sinais
Serina-Treonina Quinases TOR/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts); 60137-06-6 (Cucurbitacins); EC 2.7.- (Phosphotransferases); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.1.1 (MTOR protein, human); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i2.9



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