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[PMID]:29196817
[Au] Autor:Perotto MC; Pozzi EA; Celli MG; Luciani CE; Mitidieri MS; Conci VC
[Ad] Endereço:Instituto de Patología Vegetal (IPAVE-CIAP-INTA), Camino 60 cuadras km 5,5, Córdoba, X5020ICA, Argentina. perotto.cecilia@inta.gob.ar.
[Ti] Título:Identification and characterization of a new potyvirus infecting cucurbits.
[So] Source:Arch Virol;163(3):719-724, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.
[Mh] Termos MeSH primário: Cucurbita/virologia
Genoma Viral
Filogenia
Potyvirus/genética
RNA Viral/genética
[Mh] Termos MeSH secundário: Argentina
Sequência de Bases
Sequenciamento de Nucleotídeos em Larga Escala
Fases de Leitura Aberta
Doenças das Plantas/virologia
Potyvirus/classificação
Potyvirus/isolamento & purificação
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3660-2


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[PMID]:29320569
[Au] Autor:Guo WL; Chen BH; Chen XJ; Guo YY; Yang HL; Li XZ; Wang GY
[Ad] Endereço:School of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xin Xiang, China.
[Ti] Título:Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew.
[So] Source:PLoS One;13(1):e0190175, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cucurbit powdery mildew (PM) is one of the most severe fungal diseases, but the molecular mechanisms underlying PM resistance remain largely unknown, especially in pumpkin (Cucurbita moschata Duch.). The goal of this study was to identify gene expression differences in PM-treated plants (harvested at 24 h and 48 h after inoculation) and untreated (control) plants of inbred line "112-2" using RNA sequencing (RNA-Seq). The inbred line "112-2" has been purified over 8 consecutive generations of self-pollination and shows high resistance to PM. More than 7600 transcripts were examined in pumpkin leaves, and 3129 and 3080 differentially expressed genes (DEGs) were identified in inbred line "112-2" at 24 and 48 hours post inoculation (hpi), respectively. Based on the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway database and GO (Gene Ontology) database, a complex regulatory network for PM resistance that may involve hormone signal transduction pathways, transcription factors and defense responses was revealed at the transcription level. In addition, the expression profiles of 16 selected genes were analyzed using quantitative RT-PCR. Among these genes, the transcript levels of 6 DEGs, including bHLH87 (Basic Helix-loop-helix transcription factor), ERF014 (Ethylene response factor), WRKY21 (WRKY domain), HSF (heat stress transcription factor A), MLO3 (Mildew Locus O), and SGT1 (Suppressor of G-Two Allele of Skp1), in PM-resistant "112-2" were found to be significantly up- or down-regulated both before 9 hpi and at 24 hpi or 48 hpi; this behavior differed from that observed in the PM-susceptible material (cultivar "Jiujiangjiaoding"). The transcriptome data provide novel insights into the response of Cucurbita moschata to PM stress and are expected to be highly useful for dissecting PM defense mechanisms in this major vegetable and for improving pumpkin breeding with enhanced resistance to PM.
[Mh] Termos MeSH primário: Ascomicetos/fisiologia
Cucurbita/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Doenças das Plantas/genética
Folhas de Planta/metabolismo
[Mh] Termos MeSH secundário: Resistência à Doença
Biblioteca Gênica
Ontologia Genética
Redes e Vias Metabólicas/genética
Fotossíntese/genética
Reguladores de Crescimento de Planta/fisiologia
Folhas de Planta/microbiologia
RNA de Plantas/biossíntese
RNA de Plantas/genética
Análise de Sequência de RNA
Transdução de Sinais/genética
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Growth Regulators); 0 (RNA, Plant); 0 (Transcription Factors)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190175


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[PMID]:28854650
[Au] Autor:Shrestha D; McAuslane HJ; Adkins ST; Smith HA; Dufault N; Colee J; Webb SE
[Ad] Endereço:Department of Entomology and Nematology, University of Florida, 1881 Natural Area Dr., Steinmetz Hall, Gainesville, FL 32611.
[Ti] Título:Host-Mediated Effects of Semipersistently Transmitted Squash Vein Yellowing Virus on Sweetpotato Whitefly (Hemiptera: Aleyrodidae) Behavior and Fitness.
[So] Source:J Econ Entomol;110(4):1433-1441, 2017 Aug 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plant viruses may indirectly affect insect vector behavior and fitness via a shared host plant. Here, we evaluated the host-mediated effects of Squash vein yellowing virus (SqVYV) on the behavior and fitness of its whitefly vector, Bemisia tabaci (Gennadius) Middle East-Asia Minor 1, formerly biotype B. Alighting, settling, and oviposition behavioral assays were conducted on infected and mock-inoculated squash (Cucurbita pepo L.) and watermelon [Citrullus lanatus (Thunb) Matsum and Nakai] plants. Developmental time of immature stages, adult longevity, and fecundity were measured on infected and mock-inoculated squash plants. For adult longevity and fecundity, whiteflies were reared on infected and mock-inoculated squash plants to determine the effects of nymphal rearing host on the adult stage. More whiteflies alighted and remained settled on infected squash than on mock-inoculated squash 0.25, 1, 8, and 24 h after release. No such initial preference was observed on watermelon plants, but by 8 h after release, more whiteflies were found on mock-inoculated watermelon plants than on infected plants. Whiteflies laid approximately six times more eggs on mock-inoculated watermelon than on infected watermelon; however, no differences were observed on squash. Development from egg to adult emergence was 3 d shorter on infected than mock-inoculated squash plants. Females lived 25% longer and had higher fecundity on infected squash plants than on mock-inoculated plants, regardless of infection status of the rearing host. The host-mediated effects of SqVYV infection on whitefly behavior differ on two cucurbit host plants, suggesting the potential for more rapid spread of the virus within watermelon fields.
[Mh] Termos MeSH primário: Citrullus/virologia
Cucurbita/virologia
Hemípteros/fisiologia
Doenças das Plantas/virologia
Potyviridae/fisiologia
[Mh] Termos MeSH secundário: Animais
Tamanho Corporal
Fertilidade
Voo Animal
Aptidão Genética
Hemípteros/genética
Longevidade
Oviposição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox161


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[PMID]:28545139
[Au] Autor:Hu DW; Zhang S; Luo JY; Lü LM; Cui JJ; Zhang X
[Ad] Endereço:State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, China.
[Ti] Título:An example of host plant expansion of host-specialized Aphis gossypii Glover in the field.
[So] Source:PLoS One;12(5):e0177981, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The host plant expansion of host-specialized Aphis gossypii (Glover) has been well studied in the laboratory; however, this phenomenon is poorly understood in the field. Here, we provide a series of laboratory and field experiments to assess the role of zucchini in the host plant expansion of cotton-specialized aphids. We observed that cotton-specialized aphids possessed the ability to expand on a new host plant (cucumber), with individuals first recorded on June 12 and consequently increasing exponentially in number in a field cage. A bioassay experiment showed that aphids from both cotton and cucumber preferred their natal host, but clones from zucchini have a stronger preference for cucumber than cotton or zucchini. A total of 1512 individuals were collected from a cotton field (mixed cotton and cucurbit plot), cotton farmland (cotton alone) and a field cage and sequenced to identify their biotypes. The results for apterous individuals from the cotton field showed that more cucurbit-specialized biotypes occurred on cucumber and more cotton-specialized biotypes occurred on cotton and zucchini. A majority (> 97.0%) of aphids from both the field cage and cotton farmland were cotton-specialized individuals. Consequently, eliminating intermediate host plants may be an effective measure to suppress A. gossypii outbreaks, because cotton and cucumber are often grown together in fields and greenhouses.
[Mh] Termos MeSH primário: Afídeos/fisiologia
Cucumis sativus/parasitologia
Cucurbita/parasitologia
Gossypium/parasitologia
[Mh] Termos MeSH secundário: Agricultura/métodos
Animais
Especificidade de Hospedeiro
Interações Hospedeiro-Parasita
Dinâmica Populacional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177981


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[PMID]:28537388
[Au] Autor:Yang R; Liu Y; Zhou Z; Sheng J; Meng D
[Ad] Endereço:Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology , Tianjin 300457, People's Republic of China.
[Ti] Título:Microelectric Current Treatment Enhanced Biodegradation of Pumpkin Lignocelluloses by Trichoderma reesei RUT-C30.
[So] Source:J Agric Food Chem;65(23):4668-4675, 2017 Jun 14.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A homemade microcurrent reactor was used to treat the fermentation of Trichoderma reesei. Results indicated that the yield of saccharides for T. reesei RUT-C30 cultivated in pumpkin lignocellulose broth reaches 38.86% (w/w) when a microcurrent treatment (20 mA, at the 48th hour for 60 min) was carried out, which is significantly higher than the control group (p < 0.05). Additionally, activities of endoglucanase, cellobiohydrolase, xylanase, and pectinase were significantly increased in days 3-7. Furthermore, the fungal growth was facilitated by microelectric treatment, showing a 0.57-fold increase of spore numbers at the sixth day of cultivation. Besides, the monosaccharide composition, including glucose (1.03 mg/mL), xylose (0.12 mg/mL), arabinose (0.31 mg/mL), and fructose (0.13 mg/mL), extracted from the reactor was higher than that without the current treatment. In this work, we improved the biodegradation of lignocellulosic wastes by applying a microcurrent to lignocellulose-degrading fungal cultures and provided a new idea for the lignocellulose material pretreatment and bioconversion.
[Mh] Termos MeSH primário: Lignina/metabolismo
Trichoderma/metabolismo
Trichoderma/efeitos da radiação
[Mh] Termos MeSH secundário: Reatores Biológicos/microbiologia
Celulase/metabolismo
Cucurbita/metabolismo
Cucurbita/microbiologia
Endo-1,4-beta-Xilanases/metabolismo
Fermentação
Frutose/metabolismo
Proteínas Fúngicas/metabolismo
Glucose/metabolismo
Trichoderma/enzimologia
Trichoderma/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 11132-73-3 (lignocellulose); 30237-26-4 (Fructose); 9005-53-2 (Lignin); EC 3.2.1.4 (Cellulase); EC 3.2.1.8 (Endo-1,4-beta Xylanases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00902


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[PMID]:28517959
[Au] Autor:Sapak Z; Salam MU; Minchinton EJ; MacManus GPV; Joyce DC; Galea VJ
[Ad] Endereço:First author: Faculty of Plantation and Agrotechnology, MARA University of Technology, Shah Alam, Selangor, 40450, Malaysia; first, fifth, and sixth authors: School of Agriculture and Food Sciences, The University of Queensland, Gatton 4343, QLD, Australia; second author: Food and Agriculture Organi
[Ti] Título:POMICS: A Simulation Disease Model for Timing Fungicide Applications in Management of Powdery Mildew of Cucurbits.
[So] Source:Phytopathology;107(9):1022-1031, 2017 09.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A weather-based simulation model, called Powdery Mildew of Cucurbits Simulation (POMICS), was constructed to predict fungicide application scheduling to manage powdery mildew of cucurbits. The model was developed on the principle that conditions favorable for Podosphaera xanthii, a causal pathogen of this crop disease, generate a number of infection cycles in a single growing season. The model consists of two components that (i) simulate the disease progression of P. xanthii in secondary infection cycles under natural conditions and (ii) predict the disease severity with application of fungicides at any recurrent disease cycles. The underlying environmental factors associated with P. xanthii infection were quantified from laboratory and field studies, and also gathered from literature. The performance of the POMICS model when validated with two datasets of uncontrolled natural infection was good (the mean difference between simulated and observed disease severity on a scale of 0 to 5 was 0.02 and 0.05). In simulations, POMICS was able to predict high- and low-risk disease alerts. Furthermore, the predicted disease severity was responsive to the number of fungicide applications. Such responsiveness indicates that the model has the potential to be used as a tool to guide the scheduling of judicious fungicide applications.
[Mh] Termos MeSH primário: Simulação por Computador
Cucurbita/microbiologia
Fungicidas Industriais/administração & dosagem
Modelos Teóricos
Doenças das Plantas/microbiologia
Doenças das Plantas/prevenção & controle
[Mh] Termos MeSH secundário: Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungicides, Industrial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-11-16-0413-R


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[PMID]:28486631
[Au] Autor:Senior LJ; Missenden BP; Wright C
[Ad] Endereço:Department of Agriculture and Fisheries, Ecosciences Precinct, Dutton Park, QLD 4102, Australia.
[Ti] Título:Comparative Efficacy of Insecticides on Bactrocera tryoni and Zeugodacus cucumis (Diptera: Tephritidae) in Laboratory and Semifield Trials in Fruiting Vegetables.
[So] Source:J Econ Entomol;110(4):1619-1629, 2017 Aug 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In-field management of Bactrocera tryoni (Froggatt) and Zeugodacus cucumis (French) (Diptera: Tephritidae) in fruiting vegetable crops has relied almost exclusively on organophosphate cover sprays. Laboratory and semifield trials were performed to compare a number of alternative insecticides for efficacy against these species. A novel semifield method was used whereby the insecticides were applied to crops as cover sprays under field conditions, and treated plants bearing fruit were transferred to large cages and exposed to fruit flies. Efficacy was assessed in terms of numbers of pupae developing from treated fruit. A laboratory cage method was also used to assess effects on adult mortality and comparative effects of 1- and 3-d-aged residues. The neonicotinoids clothianidin and thiacloprid were very effective against B. tryoni and Z. cucumis. Clothianidin was the only insecticide other than dimethoate to affect adult mortality. The synthetic pyrethroid alpha-cypermethrin was also very effective, particularly in semifield trials, although higher incidence of aphid and whitefly infestation was observed in this treatment compared to others. Cyantraniliprole was effective against B. tryoni, but less effective against Z. cucumis. Imidacloprid, bifenthrin, spinetoram, and abamectin were all relatively less effective, although all demonstrated a suppressive effect.
[Mh] Termos MeSH primário: Capsicum/crescimento & desenvolvimento
Cucurbita/crescimento & desenvolvimento
Controle de Insetos
Inseticidas
Tephritidae
Verduras/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Queensland
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox109


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[PMID]:28480434
[Au] Autor:Jessica GG; Mario GL; Alejandro Z; Cesar APJ; Ivan JVE; Ruben RR; Javier AF
[Ad] Endereço:Postgrad in Experimental Biology, Division of Health and Biological Sciences (DCBS), Metropolitan Autonomous University Campus Iztapalapa (UAMI), D.F. Mexico 09340, Mexico.
[Ti] Título:CHEMICAL CHARACTERIZATION OF A HYPOGLYCEMIC EXTRACT FROM BOUCHE THAT INDUCES LIVER GLYCOGEN ACCUMULATION IN DIABETIC MICE.
[So] Source:Afr J Tradit Complement Altern Med;14(3):218-230, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The aqueous extract of ( ) fruit has demonstrated hypoglycemic effect, which may be attributed to some components in the extract. However, the major secondary metabolites in this fruit have not yet been identified and little is known about its extra-pancreatic action, in particular, on liver carbohydrate metabolism. Therefore, in addition to the isolation and structural elucidation of the principal components in the aqueous extract of , the aim of this study was to determine whether or not the hypoglycemic effect of the aqueous extract of ( ) fruit is due to accumulation of liver glycogen in diabetic mice. MATERIALS AND METHODS: The aqueous extract from fruit of was fractionated and its main secondary metabolites were purified and chemically characterized (NMR and GC-MS). Alloxan-induced diabetic mice received daily by gavage the aqueous extract (30 days). The liver glycogen content was quantified by spectroscopic method and by PAS stain; ALT and AST by spectrometric method; glycogen synthase, glycogen phosphorylase and GLUT2 by Western blot; the mRNA expression of GLUT2 and glucagon-receptor by RT-PCR; while serum insulin was quantified by ELISA method. A liver histological analysis was also performed by H&E stain. RESULTS: Chemical fingerprint showed five majoritarian compounds in the aqueous extract of : -coumaric acid, p-hydroxybenzoic acid, salicin, stigmast-7,2,2-dien-3-ol and stigmast-7-en-3-ol. The histological analysis showed accumulation of liver glycogen. Also, increased glycogen synthase and decreased glycogen phosphorylase were observed. Interestingly, the histological architecture evidenced a liver-protective effect due the extract. CONCLUSION: Five compounds were identified in aqueous extract. The hypoglycemic effect of this extract may be partially explained by liver glycogen accumulation. The bioactive compound responsible for the hypoglycemic effect of this extract will be elucidated in subsequent studies.
[Mh] Termos MeSH primário: Cucurbita/química
Diabetes Mellitus Experimental/tratamento farmacológico
Hipoglicemiantes/química
Glicogênio Hepático/metabolismo
Compostos Fitoquímicos/análise
Fitoterapia/métodos
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Aloxano
Animais
Álcoois Benzílicos/análise
Álcoois Benzílicos/farmacologia
Ácidos Cumáricos/análise
Ácidos Cumáricos/farmacologia
Diabetes Mellitus Experimental/sangue
Diabetes Mellitus Experimental/induzido quimicamente
Glucosídeos/análise
Glucosídeos/farmacologia
Hidroxibenzoatos/análise
Hidroxibenzoatos/farmacologia
Hipoglicemiantes/farmacologia
Insulina/sangue
Fígado/efeitos dos fármacos
Fígado/metabolismo
Masculino
Camundongos
Compostos Fitoquímicos/farmacologia
Extratos Vegetais/farmacologia
Propionatos
Sitosteroides/análise
Sitosteroides/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzyl Alcohols); 0 (Coumaric Acids); 0 (Glucosides); 0 (Hydroxybenzoates); 0 (Hypoglycemic Agents); 0 (Insulin); 0 (Liver Glycogen); 0 (Phytochemicals); 0 (Plant Extracts); 0 (Propionates); 0 (Sitosterols); 4649620TBZ (salicin); 6869-99-4 (stigmast-7-enol); 6SW5YHA5NG (Alloxan); IBS9D1EU3J (trans-3-(4'-hydroxyphenyl)-2-propenoic acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i3.24


  9 / 887 MEDLINE  
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[PMID]:28435133
[Au] Autor:Xanthopoulou A; Ganopoulos I; Psomopoulos F; Manioudaki M; Moysiadis T; Kapazoglou A; Osathanunkul M; Michailidou S; Kalivas A; Tsaftaris A; Nianiou-Obeidat I; Madesis P
[Ad] Endereço:Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 570 01, Greece; Lab of Genetics and Plant Breeding, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, P.O. Box 261, Thessaloniki GR-54124, Greece.
[Ti] Título:De novo comparative transcriptome analysis of genes involved in fruit morphology of pumpkin cultivars with extreme size difference and development of EST-SSR markers.
[So] Source:Gene;622:50-66, 2017 Jul 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The genetic basis of fruit size and shape was investigated for the first time in Cucurbita species and genetic loci associated with fruit morphology have been identified. Although extensive genomic resources are available at present for tomato (Solanum lycopersicum), cucumber (Cucumis sativus), melon (Cucumis melo) and watermelon (Citrullus lanatus), genomic databases for Cucurbita species are limited. Recently, our group reported the generation of pumpkin (Cucurbita pepo) transcriptome databases from two contrasting cultivars with extreme fruit sizes. In the current study we used these databases to perform comparative transcriptome analysis in order to identify genes with potential roles in fruit morphology and fruit size. Differential Gene Expression (DGE) analysis between cv. 'Munchkin' (small-fruit) and cv. 'Big Moose' (large-fruit) revealed a variety of candidate genes associated with fruit morphology with significant differences in gene expression between the two cultivars. In addition, we have set the framework for generating EST-SSR markers, which discriminate different C. pepo cultivars and show transferability to related Cucurbitaceae species. The results of the present study will contribute to both further understanding the molecular mechanisms regulating fruit morphology and furthermore identifying the factors that determine fruit size. Moreover, they may lead to the development of molecular marker tools for selecting genotypes with desired morphological traits.
[Mh] Termos MeSH primário: Cucurbita/genética
Etiquetas de Sequências Expressas
Frutas/genética
Repetições de Microssatélites
Transcriptoma
[Mh] Termos MeSH secundário: Cucurbita/crescimento & desenvolvimento
Frutas/crescimento & desenvolvimento
Polimorfismo Genético
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE


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[PMID]:28433592
[Au] Autor:Hatami M
[Ad] Endereço:Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak 38156-8-8349, Iran. Electronic address: m-hatami@araku.ac.ir.
[Ti] Título:Toxicity assessment of multi-walled carbon nanotubes on Cucurbita pepo L. under well-watered and water-stressed conditions.
[So] Source:Ecotoxicol Environ Saf;142:274-283, 2017 Aug.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The rapid increase in the production and application of various types of nanomaterials increases the possibility of their presence in total environment, which subsequently raises concerns about their potential threats to the first trophic level of organisms, specifically under varying environmental constraints. In this work, seeds of Cucurbita pepo L. were cultured in MS basal medium exposed to multi-walled carbon nanotubes (MWCNTs) at different concentrations (0, 125, 250, 500 and 1000µgmL ) under two levels of water potential, well-watered (0MPa) and water stress (-1.5MPa) induced by polyethylene glycol (PEG 6000) for 14 days. Seeds exposed to MWCNTs showed reduction in germination percentage, root and shoot length, biomass accumulation and vigor index in a dose-dependent manner. However, seedlings germinated in MWCNTs-fortified media had significantly lower germination and growth attributes than those of control under water stress conditions. This happened due to increased oxidative injury indices including hydrogen peroxide (H O ), and malondialdehyde (MDA) contents, as well as electrolyte leakage index (ELI) of tissues. The impaired morpho-physiological and biochemical processes of seedlings exposed to different concentrations of MWCNTs under both PEG-induced stress and non-stress growing conditions were consequence of changes in the activation of various cellular antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (POD). Taken together, our findings reveal that MWCNTs played negative role on seed germination and subsequent growth of C. pepo L. seedlings under both levels of water potential.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Cucurbita/efeitos dos fármacos
Nanotubos de Carbono/toxicidade
Estresse Oxidativo/efeitos dos fármacos
Poluentes do Solo/toxicidade
Água/metabolismo
[Mh] Termos MeSH secundário: Catalase/metabolismo
Cucurbita/crescimento & desenvolvimento
Cucurbita/metabolismo
Desidratação/metabolismo
Relação Dose-Resposta a Droga
Germinação/efeitos dos fármacos
Glutationa Peroxidase/metabolismo
Peróxido de Hidrogênio/metabolismo
Malondialdeído/metabolismo
Nanotubos de Carbono/química
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Plântulas/efeitos dos fármacos
Plântulas/crescimento & desenvolvimento
Plântulas/metabolismo
Sementes/efeitos dos fármacos
Sementes/crescimento & desenvolvimento
Sementes/metabolismo
Poluentes do Solo/química
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Nanotubes, Carbon); 0 (Soil Pollutants); 059QF0KO0R (Water); 4Y8F71G49Q (Malondialdehyde); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170424
[St] Status:MEDLINE



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