Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.300.522 [Categoria DeCS]
Referências encontradas : 107 [refinar]
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[PMID]:28847082
[Au] Autor:Liu H; Zhao S; Jin Y; Yue X; Deng L; Wang F; Tan T
[Ad] Endereço:Beijing Bioprocess Key Laboratory, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
[Ti] Título:Production of fumaric acid by immobilized Rhizopus arrhizus RH 7-13-9# on loofah fiber in a stirred-tank reactor.
[So] Source:Bioresour Technol;244(Pt 1):929-933, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fumaric acid is an important building-block chemical. The production of fumaric acid by fermentation is possible. Loofah fiber is a natural, biodegradable, renewable polymer material with highly sophisticated and pore structure. This work investigated a new immobilization method using loofah fiber as carrier to produce fumaric acid in a stirred-tank reactor. Compared with other carriers, loofah fiber was proven to be efficiently and successfully used in the reactor. After the optimization process, 20g addition of loofah fiber and 400rpm agitation speed were chosen as the most suitable process conditions. 30.3g/L fumaric acid in the broth as well as 19.16g fumaric acid in the precipitation of solid was achieved, while the yield from glucose reached 0.211g/g. Three batches of fermentation using the same loofah fiber carrier were conducted successfully, which meant it provided a new method to produce fumaric acid in a stirred-tank reactor.
[Mh] Termos MeSH primário: Fumaratos
Rhizopus
[Mh] Termos MeSH secundário: Reatores Biológicos
Fermentação
Luffa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fumarates); 88XHZ13131 (fumaric acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE


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[PMID]:28758630
[Au] Autor:Davis RE; Zhao Y; Wei W; Dally EL; Lee IM
[Ad] Endereço:Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, USA.
[Ti] Título:'Candidatus Phytoplasma luffae', a novel taxon associated with witches' broom disease of loofah, Luffa aegyptica Mill.
[So] Source:Int J Syst Evol Microbiol;67(8):3127-3133, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The phytoplasma associated with witches' broom disease of loofah [Luffa aegyptica Mill., syn. Luffa cylindrica (L.) M.J. Roem.] in Taiwan was classified in group 16SrVIII, subgroup A (16SrVIII-A), based on results from actual and in silico RFLP analysis of 16S rRNA gene sequences. Nucleotide sequencing of PCR-amplified, cloned DNA segments revealed rrn interoperon sequence heterogeneity in the loofah witches' broom (LfWB) phytoplasma. Whereas the 16S-23S rRNA spacer region of rrnA contained a complete tRNA-Ile gene, the spacer of rrnB contained a nonfunctional remnant of a tRNA gene. Phylogenetic analysis of the rrnA and rrnB 16S rRNA genes revealed that the LfWB phytoplasma represented a distinct lineage within the phytoplasma clade, and the LfWB phytoplasma shared less than 97.5 % nucleotide sequence similarity of 16S rRNA genes with previously described 'CandidatusPhytoplasma' taxa. Based on unique properties of DNA, we propose recognition of loofah witches' broom phytoplasma strain LfWBR as representative of a novel taxon, 'CandidatusPhytoplasma luffae'.
[Mh] Termos MeSH primário: Luffa/microbiologia
Filogenia
Phytoplasma/classificação
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
DNA Espaçador Ribossômico/genética
Genes Bacterianos
Phytoplasma/genética
Phytoplasma/isolamento & purificação
Polimorfismo de Fragmento de Restrição
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001980


  3 / 107 MEDLINE  
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[PMID]:28301290
[Au] Autor:Acosta-Rubí S; Campocosio AT; Montes-Horcasitas MDC; Quintanar-Vera L; Esparza-García F; Rodríguez-Vázquez R
[Ad] Endereço:a Department of Biotechnology and Bioengineering , Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional , Mexico City , Mexico.
[Ti] Título:Production of a halotolerant biofilm from green coffee beans immobilized on loofah fiber (Luffa cylindrica) and its effect on phenanthrene degradation in seawater.
[So] Source:J Environ Sci Health A Tox Hazard Subst Environ Eng;52(7):632-640, 2017 Jun 07.
[Is] ISSN:1532-4117
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A biofilm developed from low quality green coffee beans was tested for its capacity to degrade the polynuclear aromatic hydrocarbon (PAH), phenanthrene (Phe), in seawater. Microorganisms were immobilized on two types of Luffa cylindrica (with three and four placental cavities), and the effects of moisture content (20, 30 and 40% of water holding capacity) and particle size (<0.42 mm, 0.42-0.86 mm and 0.86-2.0 mm) of green coffee beans on microbial activity were considered. Biofilm growth determined by respirometry showed a highest microbial activity at a moisture content of 40% and particle size of 0.42-0.86 mm. The loofah fiber with three placental cavities showed the highest adherence of microorganisms. The kinetics of microbial growth in both seawater and distilled water and the scanning electron microscopies indicated that the microorganisms associated with green coffee beans are halotolerant. In fact, I-GCB-SW-G biofilm degraded 67.56% of Phe (50 mg L ) in seawater, at a significantly higher rate than in distilled water (I-GCB-DW-W).
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Café/química
Luffa/química
Fenantrenos/análise
Água do Mar/química
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Resíduos Industriais/análise
Consórcios Microbianos
Salinidade
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coffee); 0 (Industrial Waste); 0 (Phenanthrenes); 0 (Water Pollutants, Chemical); 448J8E5BST (phenanthrene)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1080/10934529.2017.1294965


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[PMID]:28243606
[Au] Autor:Zaafouri K; Ziadi M; Ben Hassen-Trabelsi A; Mekni S; Aïssi B; Alaya M; Bergaoui L; Hamdi M
[Ad] Endereço:Laboratory of Microbial Ecology and Technology (LETMi), The National Institute of Applied Sciences and Technology (INSAT), Carthage University, 2 Boulevard de la Terre, BP 676, 1080 Tunis, Tunisia.
[Ti] Título:Optimization of Hydrothermal and Diluted Acid Pretreatments of Tunisian (L.) Fibers for 2G Bioethanol Production through the Cubic Central Composite Experimental Design CCD: Response Surface Methodology.
[So] Source:Biomed Res Int;2017:9524521, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This paper opens up a new issue dealing with (LC) lignocellulosic biomass recovery in order to produce 2G bioethanol. LC fibers are composed of three principal fractions, namely, -cellulose (45.80% ± 1.3), hemicelluloses (20.76% ± 0.3), and lignins (13.15% ± 0.6). The optimization of LC fibers hydrothermal and diluted acid pretreatments duration and temperature were achieved through the cubic central composite experimental design CCD. The pretreatments optimization was monitored via the determination of reducing sugars. Then, the 2G bioethanol process feasibility was tested by means of three successive steps, namely, LC fibers hydrothermal pretreatment performed at 96°C during 54 minutes, enzymatic saccharification carried out by means of a commercial enzyme AP2, and the alcoholic fermentation fulfilled with . LC fibers hydrothermal pretreatment liberated 33.55 g/kg of reducing sugars. Enzymatic hydrolysis allowed achieving 59.4 g/kg of reducing sugars. The conversion yield of reducing sugar to ethanol was 88.66%. After the distillation step, concentration of ethanol was 1.58% with a volumetric yield about 70%.
[Mh] Termos MeSH primário: Biocombustíveis
Biotecnologia/métodos
Etanol/metabolismo
Luffa/efeitos dos fármacos
Ácidos Sulfúricos/farmacologia
Temperatura Ambiente
Água/farmacologia
[Mh] Termos MeSH secundário: Biomassa
Destilação
Fermentação/efeitos dos fármacos
Hidrólise
Lignina/metabolismo
Análise de Regressão
Espectroscopia de Infravermelho com Transformada de Fourier
Termogravimetria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Sulfuric Acids); 059QF0KO0R (Water); 11132-73-3 (lignocellulose); 3K9958V90M (Ethanol); 9005-53-2 (Lignin); O40UQP6WCF (sulfuric acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1155/2017/9524521


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[PMID]:27830359
[Au] Autor:Wang W; Wu Y; Zhang C
[Ad] Endereço:School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
[Ti] Título:High-density natural luffa sponge as anaerobic microorganisms carrier for degrading 1,1,1-TCA in groundwater.
[So] Source:Bioprocess Biosyst Eng;40(3):383-393, 2017 Mar.
[Is] ISSN:1615-7605
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Anaerobic microorganisms were applied to degrade organic contaminants in groundwater with permeable reactive barriers (PRBs). However, anaerobic microorganisms need to select optimal immobilizing material as carrier. The potential of high-density natural luffa sponge (HDLS) (a new variety of luffa) for the immobilization and protection of anaerobic microorganisms was investigated. The HDLS has a dense structure composed of a complicated interwoven fibrous network. Therefore, the abrasion rate of HDLS (0.0068 g s ) was the smallest among the four carriers [HDLS, ordinary natural luffa sponge (OLS), polyurethane sponge (PS), and gel carrier AQUAPOROUSGEL (APG)]. The results suggest that it also had the greatest water retention (10.26 H O-g dry carrier-g ) and SS retention (0.21 g dry carrier-g ). In comparison to well-established commercialized gel carrier APG, HDLS was of much better mechanical strength, hydrophilicity and stability. Microbial-immobilized HDLS also had the best performance for the remediation of 1,1,1-TCA simulated groundwater. Analysis of the clone libraries from microorganism-immobilized HDLS showed the HDLS could protect microorganisms from the toxicity of 1,1,1-TCA and maintain the stability of microbial community diversity. The mechanism of HDLS immobilizing and protecting microorganisms was proposed as follows. The HDLS had a micron-scale honeycomb structure (30-40 µm) and an irregular ravine structure (4-20 µm), which facilitate the immobilization of anaerobic microorganisms and protect the anaerobic microorganisms.
[Mh] Termos MeSH primário: Enzimas Imobilizadas/química
Água Subterrânea
Tricloroetanos/química
Microbiologia da Água
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Anaerobiose
Biodegradação Ambiental
Interações Hidrofóbicas e Hidrofílicas
Luffa
Microbiota
Microscopia Eletrônica de Varredura
Poliuretanos/química
Água/química
Poluentes Químicos da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes, Immobilized); 0 (Polyurethanes); 0 (Trichloroethanes); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); 113C650IR1 (1,1,1-trichloroethane)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE
[do] DOI:10.1007/s00449-016-1706-6


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[PMID]:27863208
[Au] Autor:Sharma D; Goel HC; Chauhan S
[Ad] Endereço:Dhara Sharma, Amity Center for Radiation Biology, Amity University, Sector-125, Noida-201303, U.P., India.
[Ti] Título:Radioprotective potential of Lagenaria siceraria extract against radiation-induced gastrointestinal injury.
[So] Source:Appl Physiol Nutr Metab;41(12):1248-1254, 2016 Dec.
[Is] ISSN:1715-5320
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:The cucurbits (prebiotics) were investigated as novel agents for radio-modification against gastrointestinal injury. The cell-cycle fractions and DNA damage were monitored in HCT-15 cells. A cucurbit extract was added to culture medium 2 h before irradiation (6 Gy) and was substituted by fresh medium at 4 h post-irradiation. The whole extract of the fruits of Lagenaria siceraria, Luffa cylindrica, or Cucurbita pepo extract enhanced G fractions (42%, 34%, and 37%, respectively) as compared with control (20%) and irradiated control (31%). With cucurbits, the comet tail length remained shorter (L. siceraria, 28 µm; L. cylindrica, 34.2 µm; C. pepo, 36.75 µm) than irradiated control (41.75 µm). For in vivo studies, L. siceraria extract (2 mg/kg body weight) was administered orally to mice at 2 h before and 4 and 24 h after whole-body irradiation (10 Gy). L. siceraria treatment restored the glutathione contents to 48.8 µmol/gm as compared with control (27.6 µmol/gm) and irradiated control (19.6 µmol/gm). Irradiation reduced the villi height from 379 to 350 µm and width from 54 to 27 µm. L. siceraria administration countered the radiation effects (length, 366 µm; width, 30 µm, respectively) and improved the villi morphology and tight junction integrity. This study reveals the therapeutic potential of cucurbits against radiation-induced gastrointestinal injury.
[Mh] Termos MeSH primário: Frutas/química
Gastroenteropatias/prevenção & controle
Lagenidium/química
Extratos Vegetais/uso terapêutico
Prebióticos
Lesões Experimentais por Radiação/prevenção & controle
Protetores contra Radiação/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Cucurbita/química
Dano ao DNA
Frutas/economia
Fase G2/efeitos da radiação
Gastroenteropatias/dietoterapia
Gastroenteropatias/metabolismo
Gastroenteropatias/patologia
Glutationa/metabolismo
Mucosa Intestinal/metabolismo
Mucosa Intestinal/patologia
Mucosa Intestinal/efeitos da radiação
Mucosa Intestinal/ultraestrutura
Luffa/química
Masculino
Camundongos
Microvilosidades/metabolismo
Microvilosidades/patologia
Microvilosidades/efeitos da radiação
Microvilosidades/ultraestrutura
Extratos Vegetais/metabolismo
Efeitos da Radiação
Lesões Experimentais por Radiação/dietoterapia
Lesões Experimentais por Radiação/metabolismo
Lesões Experimentais por Radiação/patologia
Protetores contra Radiação/metabolismo
Distribuição Aleatória
Análise de Sobrevida
Junções Íntimas/metabolismo
Junções Íntimas/patologia
Junções Íntimas/efeitos da radiação
Junções Íntimas/ultraestrutura
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Prebiotics); 0 (Radiation-Protective Agents); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


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[PMID]:27853966
[Au] Autor:Azmat R; Moin S; Saleem A
[Ad] Endereço:Department of Chemistry, University of Karachi, Karachi, 75270, Pakistan. rafiasaeed200@yahoo.com.
[Ti] Título:Remediation of Cu metal-induced accelerated Fenton reaction by potato peels bio-sorbent.
[So] Source:Environ Monit Assess;188(12):674, 2016 Dec.
[Is] ISSN:1573-2959
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This article has allied exposure to Ecological Particulate Matter (EPM) and its remediation using potato peel surface (PPC) bio-sorbent on two important edible crops Spinacia oleracea and Luffa acutangula. Fenton reaction acceleration was one of the major stress oxidation reactions as a consequence of iron and copper toxicity, which involve in the formation of hydroxyl radical (OH) through EPM. Results showed that the oxidative stress encouraged by Cu in both species that recruits the degradation of photosynthetic pigments, initiating decline in growth, reduced leaf area and degrade proteins. The plants were cultivated in natural environmental condition in three pots with three replicates like (a) control, (b) Cu treated and (c) treated water. Oxidative stress initiated by metal activity in Cu accumulated plant (b) were controlled, through bio-sorption of metal from contaminated water using PPC; arranged at laboratory scale. The acceleration of Fenton reaction was verified in terms of OH radical generation. These radicals were tested in aqueous extract of leaves of three types of plants via benzoic acid. The benzoic acid acts as a scavenger of OH radical due to which the decarboxylation of benzoic acid cured. Observation on (b) showed more rapid decarboxylation as compared to other plants which showed that Cu activity was much higher in (b) as compared to (a) and (c). The rapid decarboxylation of benzoic acid and lower chlorophyll contents in (b) suggest that Fenton reaction system was much enhanced by Cu-O and Fe-O chemistry that was successfully controlled by PPC which results in restoring the metabolic pathway and nullifying oxidative stress in
[Mh] Termos MeSH primário: Cobre/química
Peróxido de Hidrogênio/química
Ferro/química
Solanum tuberosum
Poluentes Químicos da Água/química
[Mh] Termos MeSH secundário: Ácido Benzoico/química
Cobre/toxicidade
Monitoramento Ambiental
Radical Hidroxila/química
Luffa/efeitos dos fármacos
Luffa/crescimento & desenvolvimento
Oxirredução
Estresse Oxidativo
Folhas de Planta/química
Spinacia oleracea/efeitos dos fármacos
Spinacia oleracea/crescimento & desenvolvimento
Poluentes Químicos da Água/toxicidade
Purificação da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 3352-57-6 (Hydroxyl Radical); 789U1901C5 (Copper); 8SKN0B0MIM (Benzoic Acid); BBX060AN9V (Hydrogen Peroxide); E1UOL152H7 (Iron)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE


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[PMID]:27612733
[Au] Autor:Cecen B; Kozaci LD; Yuksel M; Ustun O; Ergur BU; Havitcioglu H
[Ad] Endereço:Dokuz Eylul University, The Institute of Health Science, Department of Biomechanics, 35340 Izmir, Turkey. Electronic address: berivan.erik@deu.edu.tr.
[Ti] Título:Biocompatibility and biomechanical characteristics of loofah based scaffolds combined with hydroxyapatite, cellulose, poly-l-lactic acid with chondrocyte-like cells.
[So] Source:Mater Sci Eng C Mater Biol Appl;69:437-46, 2016 Dec 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The current study reports the biocompatibility and biomechanical characteristics of loofah-based scaffolds combined with hydroxyapatite (HA), cellulose, poly-l-lactic acid (PLLA) with chondrocytes-like cells. Scanning electron microscope (SEM) micrographs of the scaffolds showed that the addition of PLLA usually resulted in an increase in cell's attachment on scaffolds. Mechanical and elemental analyzes were assessed using tensile test and Energy Dispersive X-ray spectrometry (EDS), respectively. In summary, we showed that the loofah+PLLA+HA scaffolds perform significantly better than other loofah-based scaffolds employed in terms of increasing a diversity of mechanical properties including tensile strength and Young's modulus. Based on the analysis of the differential scanning calorimetry (DSC) thermograms and EDS spectrums that give an idea about the calcium phosphate (CaP) ratios, the improvement in the mechanical properties could principally be recognized to the strong interaction formed between loofah, PLLA and HA. The viability of chondrocytes on loofah-based scaffolds was analyzed by XTT tests. However, none of the scaffolds have proved to be toxic in metabolic activity. The histological evaluation obtained by hematoxylin and eosin (H&E), Masson trichrome, toluidine blue and immunohistochemistry methods showed that cells in all scaffolds produced extracellular matrix that defined proteoglycan and type I-II collagens. The results of this study suggest that the loofah-based scaffold with desirable properties can be considered as an ideal candidate for cartilage tissue engineering applications.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Celulose/química
Durapatita/química
Luffa/metabolismo
Poliésteres/química
[Mh] Termos MeSH secundário: Materiais Biocompatíveis/farmacologia
Varredura Diferencial de Calorimetria
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Condrócitos/citologia
Condrócitos/metabolismo
Colágeno Tipo I/metabolismo
Colágeno Tipo II/metabolismo
Seres Humanos
Imuno-Histoquímica
L-Lactato Desidrogenase/metabolismo
Microscopia Eletrônica de Varredura
Espectrometria por Raios X
Resistência à Tração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Collagen Type I); 0 (Collagen Type II); 0 (Polyesters); 459TN2L5F5 (poly(lactide)); 9004-34-6 (Cellulose); 91D9GV0Z28 (Durapatite); EC 1.1.1.27 (L-Lactate Dehydrogenase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160911
[St] Status:MEDLINE


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[PMID]:27611470
[Au] Autor:Krusong W; Kerdpiboon S; Pornpukdeewattana S; Jindaprasert A
[Ad] Endereço:Fermentation Technology Division, Faculty of Agro-Industry, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand.
[Ti] Título:Luffa sponge offsets the negative effects of aeration on bacterial cellulose production.
[So] Source:J Appl Microbiol;121(6):1665-1672, 2016 Dec.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: To offset the negative effects of aeration on bacterial cellulose (BC) production by acetic acid bacteria using enmeshed cellulose microfibrils (CM) on luffa sponge matrices (LSM). METHODS AND RESULTS: The CM were enmeshed on LSM (LSM-CM). The optimal amount of LSM-CM was determined for BC production under aerated conditions. Without LSM-CM, no BC was produced in seven out of nine production cycles at the highest aeration rate (9 l min ). However, with 0·5% LSM-CM and an aeration rate of 3 l min , a satisfactory oxygen transfer coefficient was achieved, and also a good yield of BC (5·24 g l ). Moreover, the LSM-CM was able to be recycled through nine consecutive BC production cycles. The highest BC yields (from 5·8 ± 0·4 to 6·6 ± 0·4 g l ) were associated with high bacterial biomass and this was confirmed by scanning electron microscopy. CONCLUSIONS: We confirm that LSM-CM works well as a starter. Microenvironments low in dissolved oxygen within the matrices of LSM-CM are important for BC production under aeration conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: The LSM-CM provides a microenvironment which offsets the negative effects of aeration on BC production. A sustainable, economic process for mass BC production is described using recycled LSM-CM with aeration.
[Mh] Termos MeSH primário: Celulose/biossíntese
Gluconacetobacter xylinus/metabolismo
Luffa
[Mh] Termos MeSH secundário: Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9004-34-6 (Cellulose); S88TT14065 (Oxygen)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160910
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13290


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[PMID]:27130685
[Au] Autor:Shahi A; Rai BN; Singh RS
[Ad] Endereço:Department of Chemical Engineering and Technology, Centre of Advanced Study, Indian Institute of Technology, BHU, Varanasi, 221005, India.
[Ti] Título:Analysis of Metabolites and Carbon Balance in the Biofilteration of Cumene Using Loofa Sponge as Biofilter Media.
[So] Source:Appl Biochem Biotechnol;180(2):338-48, 2016 Sep.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A laboratory-scale biofilter study was performed to treat cumene-inoculated mixed culture of bacterial community and loofa sponge (Luffa cylindrica) as support media for a period of 120 days in five distinct phases. The removal efficiency was obtained in the range of 40-85 % with maximum elimination capacity of 700 g m(-3) h(-1) at the inlet load of 1167 g m(-3) h(-1). The result demonstrated that loofa sponge is good support media for the removal of cumene at higher loading rates. Loofa sponge was characterized via chemical analysis and analytical techniques such as XRD; FTIR; XPS; and CHN, and the result obtained confirms its suitability as biofilter media. The SEM results of loofa with inoculum shows the formation of a biofilm layer on the surface of loofa. The GC-MS analysis of leachate confirms the presence of different organic compounds such as acetaldehyde and 4-hydroxy-2-oxopentanoic acids which are stable metabolites during cumene biodegradation. About 12.69 % of carbon present in inlet cumene was converted to biomass.
[Mh] Termos MeSH primário: Derivados de Benzeno/metabolismo
Carbono/metabolismo
Filtração/instrumentação
Filtração/métodos
Luffa/metabolismo
Metaboloma
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Cromatografia Gasosa-Espectrometria de Massas
Luffa/ultraestrutura
Espectroscopia de Infravermelho com Transformada de Fourier
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzene Derivatives); 7440-44-0 (Carbon); 8Q54S3XE7K (cumene)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160501
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-016-2102-z



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