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Pesquisa : B01.650.940.800.575.912.250.300.655 [Categoria DeCS]
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[PMID]:27774692
[Au] Autor:Awadallah AK; Osman ME; Ibrahim MA; Bernardes ES; Dias-Baruffi M; Konozy EH
[Ad] Endereço:Department of Zoology, Faculty of Science, University of Khartoum, Khartoum, Sudan.
[Ti] Título:Isolation and partial characterization of 3 nontoxic d-galactose-specific isolectins from seeds of Momordica balsamina.
[So] Source:J Mol Recognit;30(2), 2017 Feb.
[Is] ISSN:1099-1352
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three isolectins denoted hereforth MBaL-30, MBaL-60, and MBaL-80 were isolated from seeds extract of Momordica balsamina by 30%, 60%, and 80% ammonium sulfate saturations, respectively. The native molecular weights of these lectins, as judged by gel filtration, were 108, 56, and 160 kDa, respectively. On SDS-PAGE, under reduced condition, 27 kDa band was obtained for all isolectins. The lectins hemagglutinating activities were variably inhibited by d-galactose (minimum inhibitory concentrations = 12.5mM, 50mM, and 0.391mM, respectively). MBaL-30 and -60 could agglutinate all human blood types with slight preference for the A and O blood groups, whereas MBaL-80 did not agglutinate B and AB blood types. The 3 isolectins were purified from crude seeds extract, collectively, in a single step on the affinity matrix Lactamyl-Seralose 4B; this purified lectin fraction, which contains all isolectins, is termed MBaL. The N-terminal of MBaL till the 25th amino acid was NLSLSELDFSADTYKSFIKNLRKQL, which shares 88% sequence identity with Momordica charantia lectin type-2 ribosomal inactivating protein from Momordica charantia and 50% with momordin II from Momordica balsamina. MBaL retained 100% activity at up to 50°C for 30 minutes. MBaL-30 and MBaL-60 exhibited maximum activities in the pH range between 4 and 8, while MBaL-80 was showing maximum activity in the pH range between 3 and 5. Treatment of MBaL-30 and MBaL-60 with EDTA completely abolished their hemagglutinating activities. Addition of Zn and Fe ions to the ethylenediaminetetraacetic acid-treated MBaL-30 and MBaL-60 lectins did not only regained the loss of activity but also resulted in 200% to 300% increase in activity, respectively. MBaL-30 and -60 agglutinated gram positive Listeria monocytogenes and Staphylococcus aureus, whereas MBaL-30 could merely agglutinate Escherichia coli. None of these lectins could arrest bacterial growth. Addition of MBaL to cancer cell lines (Gastric cancer cell line (AGS) and Gastric cencer cell line (MKN45), Glioblastoma (ECV-304), and Human urinary bladder cancer cell line (U87-MG)) at varying concentrations did not cause statistically significant changes on cell growth and viability.
[Mh] Termos MeSH primário: Momordica/metabolismo
Lectinas de Plantas/análise
Sementes/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Galactose/metabolismo
Testes de Hemaglutinação
Seres Humanos
Peso Molecular
Lectinas de Plantas/química
Lectinas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Lectins); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1002/jmr.2582


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[PMID]:28926628
[Au] Autor:Birech Z; Mwangi PW; Bukachi F; Mandela KM
[Ad] Endereço:Department of Physics, University of Nairobi, P.O Box 30197-00100, Nairobi, Kenya.
[Ti] Título:Application of Raman spectroscopy in type 2 diabetes screening in blood using leucine and isoleucine amino-acids as biomarkers and in comparative anti-diabetic drugs efficacy studies.
[So] Source:PLoS One;12(9):e0185130, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Diabetes is an irreversible condition characterized by elevated blood glucose levels. Currently, there are no predictive biomarkers for this disease and the existing ones such as hemoglobin A1c and fasting blood glucose are used only when diabetes symptoms are noticed. The objective of this work was first to explore the potential of leucine and isoleucine amino acids as diabetes type 2 biomarkers using their Raman spectroscopic signatures. Secondly, we wanted to explore whether Raman spectroscopy can be applied in comparative efficacy studies between commercially available anti-diabetic drug pioglitazone and the locally used anti-diabetic herbal extract Momordica spinosa (Gilg.)Chiov. Sprague Dawley (SD) rat's blood was used and were pipetted onto Raman substrates prepared from conductive silver paste smeared glass slides. Prominent Raman bands associated with glucose (926, 1302, 1125 cm-1), leucine (1106, 1248, 1302, 1395 cm-1) and isolecucine (1108, 1248, 1437 and 1585 cm-1) were observed. The Raman bands centered at 1125 cm-1, 1395 cm-1 and 1437 cm-1 associated respectively to glucose, leucine and isoleucine were chosen as biomarker Raman peaks for diabetes type 2. These Raman bands displayed decreased intensities in blood from diabetic SD rats administered antidiabetic drugs pioglitazone and herbal extract Momordica spinosa (Gilg.)Chiov. The intensity decrease indicated reduced concentration levels of the respective biomarker molecules: glucose (1125 cm-1), leucine (1395 cm-1) and isoleucine (1437 cm-1) in blood. The results displayed the power and potential of Raman spectroscopy in rapid (10 seconds) diabetes and pre-diabetes screening in blood (human or rat's) with not only glucose acting as a biomarker but also leucine and isoleucine amino-acids where intensities of respectively assigned bands act as references. It also showed that using Raman spectroscopic signatures of the chosen biomarkers, the method can be an alternative for performing comparative efficacy studies between known and new anti-diabetic drugs. Reports on use of Raman spectroscopy in type 2 diabetes mellitus screening with Raman bands associated with leucine and isoleucine molecules acting as reference is rare in literature. The use of Raman spectroscopy in pre-diabetes screening of blood for changes in levels of leucine and isoleucine amino acids is particularly interesting as once elevated levels are noticed, necessary interventions to prevent diabetes development can be initiated.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 2/diagnóstico
Isoleucina/sangue
Leucina/sangue
Análise Espectral Raman
[Mh] Termos MeSH secundário: Animais
Biomarcadores/sangue
Glicemia/análise
Diabetes Mellitus Tipo 2/tratamento farmacológico
Diabetes Mellitus Tipo 2/veterinária
Hipoglicemiantes/química
Hipoglicemiantes/uso terapêutico
Isoleucina/química
Leucina/química
Momordica/química
Momordica/metabolismo
Extratos Vegetais/química
Extratos Vegetais/uso terapêutico
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Blood Glucose); 0 (Hypoglycemic Agents); 0 (Plant Extracts); 04Y7590D77 (Isoleucine); GMW67QNF9C (Leucine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185130


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[PMID]:28367713
[Au] Autor:Yu JS; Kim JH; Lee S; Jung K; Kim KH; Cho JY
[Ad] Endereço:* School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
[Ti] Título:Src/Syk-Targeted Anti-Inflammatory Actions of Triterpenoidal Saponins from Gac (Momordica cochinchinensis) Seeds.
[So] Source:Am J Chin Med;45(3):459-473, 2017.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Momordica cochinchinensis Spreng (family Cucurbitaceae), also known as gac, or red melon, is an edible Southeast Asian fruit valued for its nutritional and medicinal properties. Specifically, Momordicae Semen, the seeds of the gac fruit, is used in traditional Chinese medicine to treat boils, rheumatic pain, muscle spasm, hemorrhoids, and hemangiomas. In this study, a chemical investigation into a gac seed ethanol (EtOH) extract resulted in the identification of three triterpenoidal saponins (1-3), which were investigated for their anti-inflammatory effects. Among the saponins, momordica saponin I (compound 3) reduced the production of nitric oxide (NO) in LPS-activated RAW264.7 cells without inducing cytotoxicity. The mRNA levels of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 were decreased by momordica saponin I. Additionally, the translocation of p65 and p50 (subunits of the transcription factor NF-[Formula: see text]B) into the nucleus was remarkably inhibited. Furthermore, the phosphorylation levels of inflammatory signaling proteins (I[Formula: see text]B[Formula: see text], Src, and Syk) known to be upstream regulatory molecules of p65 were decreased under momordica saponin I-treated conditions. The molecular targets of momordica saponin I were confirmed in overexpression experiments and through immunoblot analyses with Src and Syk. This study provides evidence that momordica saponin I could be beneficial in treating inflammatory diseases, and should be considered a bioactive immunomodulatory agent with anti-inflammatory properties.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Momordica/química
Saponinas/farmacologia
Sementes/química
Quinase Syk/metabolismo
Triterpenos/farmacologia
Quinases da Família src/metabolismo
[Mh] Termos MeSH secundário: Animais
Núcleo Celular/metabolismo
Ciclo-Oxigenase 2/metabolismo
Camundongos
NF-kappa B/metabolismo
Proteínas de Neoplasias/metabolismo
Óxido Nítrico/metabolismo
Óxido Nítrico Sintase Tipo II/metabolismo
Proteínas de Transporte Nucleocitoplasmático/metabolismo
Fosforilação/efeitos dos fármacos
Células RAW 264.7
Saponinas/isolamento & purificação
Triterpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (NF-kappa B); 0 (Neoplasm Proteins); 0 (Nucleocytoplasmic Transport Proteins); 0 (Saponins); 0 (Triterpenes); 0 (p65 oncofetal mRNA transport protein, rat); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.99.1 (Cyclooxygenase 2); EC 2.7.10.2 (Syk Kinase); EC 2.7.10.2 (src-Family Kinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X17500288


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[PMID]:28322091
[Au] Autor:Chung IM; Rekha K; Rajakumar G; Thiruvengadam M
[Ad] Endereço:Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University , Seoul - 143 701 , Republic of Korea.
[Ti] Título:Jasmonic and salicylic acids enhanced phytochemical production and biological activities in cell suspension cultures of spine gourd (Momordica dioica Roxb).
[So] Source:Acta Biol Hung;68(1):88-100, 2017 Mar.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:In vitro cell suspension culture was established for the production of commercially valuable phytochemicals in Momordica dioica. The influence of elicitors in jasmonic acid (JA) and salicylic acid (SA) increased their effect on phytochemical production and biomass accumulation in M. dioica. The results indicate that compared with non-elicited cultures, JA- and SA-elicited cell suspension cultures had significantly enhanced phenolic, flavonoid, and carotenoid production, as well as antioxidant, antimicrobial, and antiproliferative activities. Furthermore, elicited cultures produced 22 phenolic compounds, such as flavonols, hydroxycinnamic acids, and hydroxybenzoic acids. Greater biomass production, phytochemical accumulation, and biological activity occurred in JA- than in SA-elicited cell cultures. This study is the first to successfully establish M. dioica cell suspension cultures for the production of phenolic compounds and carotenoids, as well as for biomass accumulation.
[Mh] Termos MeSH primário: Ciclopentanos/farmacologia
Momordica/efeitos dos fármacos
Oxilipinas/farmacologia
Compostos Fitoquímicos/metabolismo
Ácido Salicílico/farmacologia
[Mh] Termos MeSH secundário: Anti-Infecciosos/metabolismo
Anti-Infecciosos/farmacologia
Antioxidantes/metabolismo
Antioxidantes/farmacologia
Bactérias/efeitos dos fármacos
Bactérias/crescimento & desenvolvimento
Biomassa
Carotenoides/metabolismo
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Cromatografia Líquida de Alta Pressão/métodos
Relação Dose-Resposta a Droga
Flavonoides/metabolismo
Fungos/efeitos dos fármacos
Fungos/crescimento & desenvolvimento
Células HT29
Seres Humanos
Células MCF-7
Momordica/química
Momordica/citologia
Fenóis/metabolismo
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Reguladores de Crescimento de Planta/farmacologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Cyclopentanes); 0 (Flavonoids); 0 (Oxylipins); 0 (Phenols); 0 (Phytochemicals); 0 (Plant Extracts); 0 (Plant Growth Regulators); 36-88-4 (Carotenoids); 6RI5N05OWW (jasmonic acid); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.1.8


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[PMID]:28054163
[Au] Autor:Rao LX; Guo Y; Zhang LL; Zhou XP; Hong J; Wu JX
[Ad] Endereço:State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, People's Republic of China.
[Ti] Título:Genetic variation and population structure of Cucumber green mottle mosaic virus.
[So] Source:Arch Virol;162(5):1159-1168, 2017 May.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Cucumber green mottle mosaic virus (CGMMV) is a single-stranded, positive sense RNA virus infecting cucurbitaceous plants. In recent years, CGMMV has become an important pathogen of cucurbitaceous crops including watermelon, pumpkin, cucumber and bottle gourd in China, causing serious losses to their production. In this study, we surveyed CGMMV infection in various cucurbitaceous crops grown in Zhejiang Province and in several seed lots purchased from local stores with the dot enzyme-linked immunosorbent assay (dot-ELISA), using a CGMMV specific monoclonal antibody. Seven CGMMV isolates obtained from watermelon, grafted watermelon or oriental melon samples were cloned and sequenced. Identity analysis showed that the nucleotide identities of the seven complete genome sequences ranged from 99.2 to 100%. Phylogenetic analysis of seven CGMMV isolates as well as 24 other CGMMV isolates from the GenBank database showed that all CGMMV isolates could be grouped into two distinct monophyletic clades according to geographic distribution, i.e. Asian isolates for subtype I and European isolates for subtype II, indicating that population diversification of CGMMV isolates may be affected by geographical distribution. Site variation rate analysis of CGMMV found that the overall variation rate was below 8% and mainly ranged from 2 to 5%, indicating that the CGMMV genomic sequence was conservative. Base substitution type analysis of CGMMV showed a mutational bias, with more transitions (A↔G and C↔T) than transversions (A↔C, A↔T, G↔C and G↔T). Most of the variation occurring in the CGMMV genome resulted in non-synonymous substitutions, and the variation rate of some sites was higher than 30% because of this mutational bias. Selection constraint analysis of CGMMV ORFs showed strong negative selection acting on the replication-associated protein, similar to what occurs for other plant RNA viruses. Finally, potential recombination analysis identified isolate Ec as a recombinant with a low degree of confidence.
[Mh] Termos MeSH primário: Citrullus/virologia
Cucumis sativus/virologia
Cucurbita/virologia
Variação Genética/genética
Genoma Viral/genética
Momordica/virologia
Tobamovirus/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Doenças das Plantas/virologia
RNA Viral/genética
Análise de Sequência de DNA
Tobamovirus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3207-y


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[PMID]:27435184
[Au] Autor:Chuyen HV; Roach PD; Golding JB; Parks SE; Nguyen MH
[Ad] Endereço:School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW, 2258, Australia.
[Ti] Título:Effects of four different drying methods on the carotenoid composition and antioxidant capacity of dried Gac peel.
[So] Source:J Sci Food Agric;97(5):1656-1662, 2017 Mar.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Gac fruit (Momordica cochinchinensis Spreng.) is a rich source of carotenoids for the manufacture of powder, oil and capsules for food, cosmetic and pharmaceutical uses. Currently, only the aril of the Gac fruit is processed and the peel, similar to the other components, is discarded, although it contains high level of carotenoids, which could be extracted for commercial use. In the present study, four different drying methods (hot-air, vacuum, heat pump and freeze drying), different temperatures and drying times were investigated for producing dried Gac peel suitable for carotenoid extraction. RESULTS: The drying methods and drying temperatures significantly affected the drying time, carotenoid content and antioxidant capacity of the dried Gac peel. Among the investigated drying methods, hot-air drying at 80 C and vacuum drying at 50 C produced dried Gac peel that exhibited the highest retention of carotenoids and the strongest antioxidant capacity. CONCLUSION: Hot-air drying at 80 C and vacuum drying at 50 C are recommended for the drying of Gac peel. © 2016 Society of Chemical Industry.
[Mh] Termos MeSH primário: Antioxidantes/análise
Carotenoides/análise
Dessecação/métodos
Momordica/química
[Mh] Termos MeSH secundário: Frutas/química
Temperatura Alta
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.7918


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[PMID]:27591010
[Au] Autor:Ramalhete C; Mulhovo S; Molnar J; Ferreira MU
[Ad] Endereço:Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisbon, Portugal. Electronic address: catiar@uatlantica.pt.
[Ti] Título:Triterpenoids from Momordica balsamina: Reversal of ABCB1-mediated multidrug resistance.
[So] Source:Bioorg Med Chem;24(21):5061-5067, 2016 Nov 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ability as P-glycoprotein (P-gp, ABCB1) modulators of thirty (1-30) triterpenoids of the cucurbitane-type was evaluated on human L5178 mouse T-lymphoma cell line transfected with the human MDR1 gene, through the rhodamine-123 exclusion assay. Compounds (1-26, and 29, 30) were previously obtained from the African medicinal plant Momordica balsamina, through both isolation (1-15) and molecular derivatization (16-26 and 29, 30). Compounds 27-28 are two new karavilagenin C (34) derivatives having succinic acid moieties. Apart from 4, 6, 8, 10 and 11, most of the isolated compounds (1-15) displayed strong MDR reversing activity in a dose-dependent mode, exhibiting a many-fold activity when compared with verapamil, used as positive control. At the lowest concentration tested, compounds 2 and 7 were the most active. However, a decrease of activity was found for the acyl derivatives (16-30). In a chemosensitivity assay, the MDR reversing activity of some of the most active compounds (1-3, 5, 7, 12-15) was further assessed on the same cell model. All the tested compounds, excepting 15, corroborated the results of the transport assay, revealing to synergistically interact with doxorubicin. Structure-activity relationship studies, taking into account previous results, showed that different substitution patterns, at both the tetracyclic nucleus and the side chain, play important role in ABCB1 reversal activity. An optimal lipophilicity was also recognized.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Resistência a Múltiplos Medicamentos/efeitos dos fármacos
Momordica/química
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Subfamília B de Transportador de Cassetes de Ligação de ATP/antagonistas & inibidores
Animais
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Proliferação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Camundongos
Conformação Molecular
Relação Estrutura-Atividade
Triterpenos/química
Triterpenos/isolamento & purificação
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCB1 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family B); 0 (Antineoplastic Agents, Phytogenic); 0 (Triterpenes)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160904
[St] Status:MEDLINE


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[PMID]:27534102
[Au] Autor:Fan R; Cheng RR; Zhu HT; Wang D; Yang CR; Xua M; Zhang YJ
[Ti] Título:Two New Oleanane-type Triterpenoids from Methanolyzed Saponins of Momordica cochinchinensis.
[So] Source:Nat Prod Commun;11(6):725-8, 2016 Jun.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two new oleanane-type triterpenoid saponins (1 and 2) were isolated from the methanolyzed total saponins of the seeds of Momordica cochinchinensis (Lour.) Spreng, together with 16 known compounds (3-18). Their structures were elucidated on the basis of detailed spectroscopic, including 1D and 2D NMR, mass spectrometric, methanolysis and LC-MS analysis. All the isolates were tested for their cytotoxic activities against five human cancer cell lines (HL-60, SMMC-7721, PANC-1, A-549, and SW-480) and the glucose uptake activity. The known compound 6 exhibited toxic effects against HL-60 with an IC50 value of 18.1 µM, while 10 showed cytotoxicity against SMMC-7721 and A-549 cell lines, with IC50 values of 34.4 and 32.8 µM, respectively. In addition, the new compound 2 showed glucose uptake activity with a glucose consumption value of 0.29 µM at 10 µM concentration.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Medicamentos de Ervas Chinesas/farmacologia
Momordica/química
Ácido Oleanólico/análogos & derivados
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Linhagem Celular Tumoral
Ensaios de Seleção de Medicamentos Antitumorais
Medicamentos de Ervas Chinesas/química
Seres Humanos
Estrutura Molecular
Ácido Oleanólico/química
Ácido Oleanólico/farmacologia
Saponinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Drugs, Chinese Herbal); 0 (Saponins); 0 (oleanane); 6SMK8R7TGJ (Oleanolic Acid)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160818
[Lr] Data última revisão:
160818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160819
[St] Status:MEDLINE


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[PMID]:27442534
[Au] Autor:Chowdappa P; Kumar BJ; Kumar SP; Madhura S; Bhargavi BR; Lakshmi MJ
[Ad] Endereço:First author: Director, Central Plantation Crops Research Institute, Kasaragod, Kerala; and first, second, third, fourth, fifth, and sixth authors: Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore-560089, India.
[Ti] Título:Population Structure of Phytophthora nicotianae Reveals Host-Specific Lineages on Brinjal, Ridge Gourd, and Tomato in South India.
[So] Source:Phytopathology;106(12):1553-1562, 2016 12.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Severe outbreaks of Phytophthora fruit rot on brinjal, ridge gourd, and tomato have been observed since 2011 in Andhra Pradesh, Karnataka, Telangana, and Tamil Nadu states of India. Therefore, 76 Phytophthora nicotianae isolates, recovered from brinjal (17), ridge gourd (40), and tomato (19) from different localities in these states during the June to December cropping season of 2012 and 2013, were characterized based on phenotypic and genotypic analyses and aggressiveness on brinjal, tomato, and ridge gourd. All brinjal and ridge gourd isolates were A2, while tomato isolates were both A1 (13) and A2 (6). All isolates were metalaxyl sensitive. In addition, isolates were genotyped for three mitochondrial (ribosomal protein L5-small subunit ribosomal RNA [rpl5-rns], small subunit ribosomal RNA-cytochrome c oxidase subunit 2 [rns-cox2], and cox2+spacer) and three nuclear loci (hypothetical protein [hyp], scp-like extracellular protein [scp], and beta-tubulin [ß-tub]). All regions were polymorphic but nuclear regions were more variable than mitochondrial regions. The network analysis of genotypes using the combined dataset of three nuclear regions revealed a host-specific association. However, the network generated using mitochondrial regions limited such host-specific groupings only to brinjal isolates. P. nicotianae isolates were highly aggressive and produced significantly (P ≤ 0.01) larger lesions on their respective host of origin than on other hosts. The results indicate significant genetic variation in the population of P. nicotianae, leading to identification of host-specific lineages responsible for severe outbreaks on brinjal, ridge gourd, and tomato.
[Mh] Termos MeSH primário: Variação Genética
Lycopersicon esculentum/parasitologia
Momordica/parasitologia
Phytophthora/fisiologia
Doenças das Plantas/parasitologia
Solanum melongena/parasitologia
[Mh] Termos MeSH secundário: DNA Mitocondrial/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Genética Populacional
Genótipo
Geografia
Índia
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160722
[St] Status:MEDLINE


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[PMID]:27319013
[Au] Autor:Ajji PK; Walder K; Puri M
[Ad] Endereço:Bioprocessing Laboratory, Centre for Chemistry and Biotechnology, School of Life and Environment Sciences, Deakin University, 75 Pigdons Road, Waurn Ponds, Geelong, 3220, Victoria, Australia.
[Ti] Título:Functional Analysis of a Type-I Ribosome Inactivating Protein Balsamin from Momordica balsamina with Anti-Microbial and DNase Activity.
[So] Source:Plant Foods Hum Nutr;71(3):265-71, 2016 Sep.
[Is] ISSN:1573-9104
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ribosome inactivating proteins (RIPs) have received considerable attention in biomedical research because of their unique activities towards tumor and virus-infected cells. We extracted balsamin, a type-I RIP, from Momordica balsamina. In the present study, a detailed investigation on DNase activity, antioxidant capacity and antibacterial activity was conducted using purified balsamin. DNase-like activity of balsamin towards plasmid DNA was pH, incubation time and temperature dependent. Moreover, the presence of Mg(2+) (10-50 mM) influenced the DNA cleavage activity. Balsamin also demonstrated reducing power and a capacity to scavenge free radicals in a dose dependent manner. Furthermore, the protein exhibited antibacterial activity against Staphylococcus aureus, Salmonella enterica, Staphylococcus epidermidis and Escherichia coli, which suggests potential utility of balsamin as a nutraceutical.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Desoxirribonucleases/antagonistas & inibidores
Momordica/química
Proteínas de Plantas/farmacologia
Proteínas Inativadoras de Ribossomos/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/análise
Antioxidantes/análise
Antioxidantes/farmacologia
Escherichia coli/efeitos dos fármacos
Concentração de Íons de Hidrogênio
Magnésio/metabolismo
Extratos Vegetais/análise
Extratos Vegetais/farmacologia
Proteínas de Plantas/análise
Proteínas Inativadoras de Ribossomos/análise
Salmonella enterica/efeitos dos fármacos
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus epidermidis/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Plant Extracts); 0 (Plant Proteins); 0 (balsamin); EC 3.1.- (Deoxyribonucleases); EC 3.2.2.22 (Ribosome Inactivating Proteins); I38ZP9992A (Magnesium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160620
[St] Status:MEDLINE
[do] DOI:10.1007/s11130-016-0555-4



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