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[PMID]: 28854906 [Au] Autor: Hasan MM; Ahmed QU; Soad SZM; Latip J; Taher M; Syafiq TMF; Sarian MN; Alhassan AM; Zakaria ZA [Ad] Endereço: Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Pahang DM, 25200, Kuantan, Malaysia. [Ti] Título: Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cells. [So] Source: BMC Complement Altern Med;17(1):431, 2017 Aug 30. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Tetracera indica Merr. (Family: Dilleniaceae), known to the Malay as 'Mempelas paya', is one of the medicinal plants used in the treatment of diabetes in Malaysia. However, no proper scientific study has been carried out to verify the traditional claim of T. indica as an antidiabetic agent. Hence, the aims of the present study were to determine the in vitro antidiabetic potential of the T. indica stems ethanol extract, subfractions and isolated compounds. METHODS: The ethanol extract and its subfractions, and isolated compounds from T. indica stems were subjected to cytotoxicity test using MTT viability assay on 3T3-L1 pre-adipocytes. Then, the test groups were subjected to the in vitro antidiabetic investigation using 3T3-L1 pre-adipocytes and differentiated adipocytes to determine the insulin-like and insulin sensitizing activities. Rosiglitazone was used as a standard antidiabetic agent. All compounds were also subjected to fluorescence glucose (2-NBDG) uptake test on differentiated adipocytes. Test solutions were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails, which were modified by the addition of compounds to be investigated and in the presence or absence of insulin. Isolation of bioactive compounds from the most effective subfraction (ethyl acetate) was performed through repeated silica gel and sephadex LH-20 column chromatographies and their structures were elucidated through H-and C-NMR spectroscopy. RESULTS: Four monoflavonoids, namely, wogonin, norwogonin, quercetin and techtochrysin were isolated from the T. indica stems ethanol extract. Wogonin, norwogonin and techtochrysin induced significant (P < 0.05) adipogenesis like insulin and enhanced adipogenesis like rosiglitazone. Wogonin and norwogonin also exhibited significant (P < 0.05) glucose uptake activity. CONCLUSION: The present study demonstrated that the flavonoids isolated from the T. indica stems possess antidiabetic potential revealing insulin-like and insulin-sensitizing effects which were significant among the compounds. This also rationalizes the traditional use of T. indica in the management of diabetes in Malaysia. [Mh] Termos MeSH primário: Adipócitos/efeitos dos fármacos Adipogenia/efeitos dos fármacos Dilleniaceae/química Flavonoides/farmacologia Glucose/metabolismo Hipoglicemiantes/farmacologia Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Células 3T3-L1 Adipócitos/citologia Adipócitos/metabolismo Animais Transporte Biológico/efeitos dos fármacos Hipoglicemiantes/isolamento & purificação Insulina/metabolismo Camundongos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Flavonoids); 0 (Hypoglycemic Agents); 0 (Insulin); 0 (Plant Extracts); IY9XDZ35W2 (Glucose) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170913 [Lr] Data última revisão: 170913 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170901 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-017-1929-3

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[PMID]: 28490129 [Au] Autor: Biswas R; Chanda J; Kar A; Mukherjee PK [Ad] Endereço: School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, India. Electronic address: rajarshi_mpharm@rediff.com. [Ti] Título: Tyrosinase inhibitory mechanism of betulinic acid from Dillenia indica. [So] Source: Food Chem;232:689-696, 2017 Oct 01. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The fruit of Dillenia indica L. is extensively used as a food additive. Betulinic acid (BA) is the most prominent secondary metabolite present in D. indica. This study screened the bioassay guided isolation of BA from D. indica and explored its tyrosinase inhibitory mechanism. Half maximal inhibitory concentration (IC ) of BA were calculated as 13.93µM and 25.66µM for diphenolase and monophenolase. Enzyme kinetic analysis revealed that BA inhibited tyrosinase activity non-competitively. Further, conformational analysis of tyrosinase with BA was measured by fluorescence and circular dichroism spectroscopy. These results implied that diminish rigidity of enzyme might disturb the catalytic conformation of tyrosinase. Moreover, In-silico analysis confirmed probable binding polar and non-polar region on the active site of tyrosinase. Based on these findings, we suggest that BA from D. indica may be useful in preventing enzymatic browning reactions in food products. [Mh] Termos MeSH primário: Dilleniaceae Monofenol Mono-Oxigenase [Mh] Termos MeSH secundário: Agaricales Inibidores Enzimáticos Cinética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Enzyme Inhibitors); EC 1.14.18.1 (Monophenol Monooxygenase) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170731 [Lr] Data última revisão: 170731 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170512 [St] Status: MEDLINE

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[PMID]: 28259017 [Au] Autor: Mohanty AS; Jena BS [Ad] Endereço: CSIR-Institute of Minerals and Materials Technology, Bhubaneswar 751 013, Odisha, India; Academy of Scientific and Innovative Research, New Delhi, India. [Ti] Título: Innate catalytic and free radical scavenging activities of silver nanoparticles synthesized using Dillenia indica bark extract. [So] Source: J Colloid Interface Sci;496:513-521, 2017 06 15. [Is] ISSN: 1095-7103 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A green approach was envisaged for the rapid synthesis of stable silver nanoparticles in an aqueous medium using phenolic rich ethanolic bark extract from D. indica with marked free radical scavenging and reducing ability. Biosynthesis of silver nanoparticles (AgNPs) was confirmed and characterized by using UV-visible spectroscopy, particle size analyzer, X-ray diffractometry (XRD), Transmission Electron Microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FT-IR). Bio-reduction of Ag+ was confirmed with the appearance of golden yellow coloration within 5-10min at 45°C with maximum absorbance at 421nm. XRD analysis of AgNPs indicated the crystalline nature of metallic Ag. As analyzed by TEM, AgNPs were found to be spherical in shape, well dispersed and size varied from 15 to 35nm and dynamic light scattering (DLS) studies showed the average particle size of 29nm with polydispersity index (PDI) of 0.280. Synthesized AgNPs were showing surface functionalization as revealed through FTIR studies. These AgNPs were observed to be highly stable at room temperature (28±2°C) for more than 3months, thereby indicating the ethanolic extract of D. indica was a reducing as well as a capping agent for stabilization of AgNPs. Moreover, these green synthesized AgNPs showed enhanced free radical scavenging and excellent catalytic activities when used in the reduction of 4-nitrophenol and methylene blue dye, at room temperature. [Mh] Termos MeSH primário: Dilleniaceae/química Depuradores de Radicais Livres/química Nanopartículas Metálicas/química Casca de Planta/química Extratos Vegetais/química Prata/química [Mh] Termos MeSH secundário: Catálise Química Verde/métodos Azul de Metileno/química Nitrofenóis/química Oxirredução Tamanho da Partícula Fenóis/química [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Free Radical Scavengers); 0 (Nitrophenols); 0 (Phenols); 0 (Plant Extracts); 3M4G523W1G (Silver); T42P99266K (Methylene Blue); Y92ZL45L4R (4-nitrophenol) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170830 [Lr] Data última revisão: 170830 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170305 [St] Status: MEDLINE

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[PMID]: 27951742 [Au] Autor: Kviecinski MR; David IM; Fernandes FS; Correa MD; Clarinda MM; Freitas AF; Silva JD; Gava M; Müller SD; Florentino D; Petronilho F; Moterle D; Kanis LA; Pedrosa RC [Ad] Endereço: a Post-Graduate Program in Health Sciences , Universidade do Sul de Santa Catarina , Palhoça , Brazil. [Ti] Título: Healing effect of Dillenia indica fruit extracts standardized to betulinic acid on ultraviolet radiation-induced psoriasis-like wounds in rats. [So] Source: Pharm Biol;55(1):641-648, 2017 Dec. [Is] ISSN: 1744-5116 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: CONTEXT: Dillenia indica Linn. (Dilleniaceae) is traditionally used to treat skin inflammation. OBJECTIVE: This study evaluated the healing effect of Dillenia indica fruit extracts on induced psoriasis-like wounds in Wistar rats. MATERIALS AND METHODS: Extracts were standardized to betulinic acid, including an aqueous ethanolic extract (AEE), ethyl acetate extract (EAE) and petroleum ether extract. Effects against lipid peroxidation were assessed in vitro. Wounds were created at rat tails (n = 12). Topical treatments were applied once daily for 7 days (1 mL of AEE or EAE at 5 or 50 mg/mL). Maximal dose was defined by the extract solubility. A 10-fold lower dose was also tested. Positive and negative controls were treated with clobetasol (0.5 mg/mL) or excipient. Half of each group was euthanized for histology. The remaining animals were observed for 20 days for wound measurements. RESULTS: Yields of AEE and EAE were 4.3 and 0.7%, respectively. Betulinic acid concentrations in AEE and EAE were 4.6 and 107.6 mg/g. Extracts neutralized lipid peroxidation in vitro at 0.02 µg/mL, accelerating healing at 50 mg/mL. Complete healing in mice treated with AEE occurred 16 days after wound induction. This time was 14 and 12 days in mice treated with EAE and clobetasol. Compared to orthokeratosis, parakeratosis was reduced by AEE (25%), EAE (45%) and clobetasol (55%). EAE caused superior protection against biomolecules oxidation of skin compared to AEE. DISCUSSION AND CONCLUSION: EAE exhibited activity closer to that of clobetasol. Betulinic acid may be an active constituent, which should be assessed in future studies. [Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia Antioxidantes/farmacologia Fármacos Dermatológicos/farmacologia Dilleniaceae/química Frutas/química Extratos Vegetais/farmacologia Psoríase/tratamento farmacológico Pele/efeitos dos fármacos Triterpenos/farmacologia Raios Ultravioleta Cicatrização/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Anti-Inflamatórios/isolamento & purificação Anti-Inflamatórios/normas Antioxidantes/isolamento & purificação Antioxidantes/normas Biomarcadores/metabolismo Clobetasol/farmacologia Fármacos Dermatológicos/isolamento & purificação Fármacos Dermatológicos/normas Modelos Animais de Doenças Peroxidação de Lipídeos/efeitos dos fármacos Masculino Malondialdeído/metabolismo Estresse Oxidativo/efeitos dos fármacos Fitoterapia Extratos Vegetais/isolamento & purificação Extratos Vegetais/normas Plantas Medicinais Carbonilação Proteica/efeitos dos fármacos Psoríase/etiologia Psoríase/metabolismo Psoríase/patologia Ratos Wistar Pele/metabolismo Pele/patologia Solventes/química Fatores de Tempo Triterpenos/isolamento & purificação Triterpenos/normas [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Biomarkers); 0 (Dermatologic Agents); 0 (Plant Extracts); 0 (Solvents); 0 (Triterpenes); 4G6A18707N (betulinic acid); 4Y8F71G49Q (Malondialdehyde); ADN79D536H (Clobetasol) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170313 [Lr] Data última revisão: 170313 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161214 [St] Status: MEDLINE

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[PMID]: 27889244 [Au] Autor: Sabandar CW; Jalil J; Ahmat N; Aladdin NA [Ad] Endereço: Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia (UKM), Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. [Ti] Título: Medicinal uses, chemistry and pharmacology of Dillenia species (Dilleniaceae). [So] Source: Phytochemistry;134:6-25, 2017 Feb. [Is] ISSN: 1873-3700 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The genus Dillenia is comprised of about 100 species of evergreen and deciduous trees or shrubs of disjunct distribution in the seasonal tropics of Madagascar through South and South East Asia, Malaysia, North Australia, and Fiji. Species from this genus have been widely used in medicinal folklore to treat cancers, wounds, jaundice, fever, cough, diabetes mellitus, and diarrhea as well as hair tonics. The plants of the genus also produce edible fruits and are cultivated as ornamental plants. Flavonoids, triterpenoids, and miscellaneous compounds have been identified in the genus. Their extracts and pure compounds have been reported for their antimicrobial, anti-inflammatory, cytotoxic, antidiabetes, antioxidant, antidiarrheal, and antiprotozoal activities. Mucilage from their fruits is used in drug formulations. [Mh] Termos MeSH primário: Dilleniaceae/química Plantas Medicinais/química [Mh] Termos MeSH secundário: Anti-Infecciosos/uso terapêutico Antioxidantes/uso terapêutico Austrália Fiji Flavonoides/química Flavonoides/isolamento & purificação Flavonoides/uso terapêutico Madagáscar Malásia Extratos Vegetais/química Triterpenos/química Triterpenos/isolamento & purificação Triterpenos/uso terapêutico [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Flavonoids); 0 (Plant Extracts); 0 (Triterpenes) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170306 [Lr] Data última revisão: 170306 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161128 [St] Status: MEDLINE

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[PMID]: 27864111 [Au] Autor: Fomogne-Fodjo MC; Ndinteh DT; Olivier DK; Kempgens P; van Vuuren S; Krause RW [Ad] Endereço: Department of Chemistry, Rhodes University, PO Box 94, Grahamstown 6140, South Africa. Electronic address: cy.fomogne@gmail.com. [Ti] Título: Secondary metabolites from Tetracera potatoria stem bark with anti-mycobacterial activity. [So] Source: J Ethnopharmacol;195:238-245, 2017 Jan 04. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Tetracera potatoria Afzel. Exg. Don (Dilleniaceae) is a medicinal plant used traditionally in Africa for the treatment of tuberculosis related ailments and respiratory infections. The antibacterial activity of the medium polar extracts of T. potatoria leaves and stem bark was recently reported against Mycobacterium smegmatis (MIC 25µg/mL) and M. aurum (65µg/mL), two fast-growing Mycobacterium strains used as model micro-organisms for the more pathogenic strain Mycobacterium tuberculosis (Fomogne-Fodjo et al., 2014). The aim of this study was consequently to isolate the compounds possibly contributing to this activity, and which may therefore be promising precursors to be used for the development of novel anti-TB drugs. MATERIALS AND METHODS: T. potatoria medium polar extract [MeOH/DCM (1:1, v/v)] was fractionated sequentially with petroleum ether to which EtOAC and MeOH were gradually added to increase the polarity. The examination of T. potatoria extract and its fractions was guided by bioassays for anti-mycobacterial activity against M. smegmatis (ATCC 23246) and M. aurum (NCTC 10437) using the minimum inhibitory concentration (MIC) method. All the isolated compounds were structurally elucidated using spectroscopic techniques and evaluated for their anti-mycobacterial activity. RESULTS: Two novel secondary metabolites (1, 2) named tetraceranoate and N-hydroxy imidate-tetracerane, together with five known compounds [ß-stigmasterol (3), stigmast-5-en-3ß-yl acetate (4), betulinic acid (5), betulin (6) and lupeol (7)] were isolated and identified. Tetraceranoate exhibited the best activity against M. smegmatis with a minimum inhibitory concentration (MIC) of 7.8µg/mL, while ß-stigmasterol, betulinic acid and betulin showed appreciable anti-mycobacterial activity against both strains (MIC 15µg/mL). CONCLUSION: Seven compounds were isolated from the medium polar extract [MeOH/DCM (1:1, v/v)] of T. potatoria stem bark. Only tetraceranoate one of the isolated compounds showed antibacterial activity against M. smegmatis having efficacy as high as rifampicin (one of a three drug regimen recommended in the initial phase short-course anti-tuberculosis therapy). Thus, tetraceranoate might be an interesting target for systematic testing of anti-TB treatment and management. This research supports the use of T. potatoria in African traditional medicine for the treatment of tuberculosis related symptoms. [Mh] Termos MeSH primário: Antituberculosos/farmacologia Dilleniaceae/metabolismo Mycobacterium smegmatis/efeitos dos fármacos Mycobacterium tuberculosis/efeitos dos fármacos Casca de Planta/metabolismo Caules de Planta/metabolismo [Mh] Termos MeSH secundário: Antituberculosos/química Antituberculosos/isolamento & purificação Fracionamento Químico Testes de Sensibilidade Microbiana Estrutura Molecular Mycobacterium smegmatis/crescimento & desenvolvimento Mycobacterium tuberculosis/crescimento & desenvolvimento Fitoterapia Plantas Medicinais Rifampina/farmacologia Solventes/química Relação Estrutura-Atividade [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antitubercular Agents); 0 (Solvents); VJT6J7R4TR (Rifampin) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170703 [Lr] Data última revisão: 170703 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161120 [St] Status: MEDLINE

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[PMID]: 27378435 [Au] Autor: Nayak PK; Rayaguru K; Radha Krishnan K [Ad] Endereço: Department of FET, Central Institute of Technology, Kokrajhar, Assam, 783370, India. [Ti] Título: Quality comparison of elephant apple juices after high-pressure processing and thermal treatment. [So] Source: J Sci Food Agric;97(5):1404-1411, 2017 Mar. [Is] ISSN: 1097-0010 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: In the present work, the effect of high pressure processing (HPP) on the quality parameters (pH, °Brix, total acidity, viscosity, colour, antioxidant activity, total phenols, total flavonoids, microbial flora, and sensory analysis) of elephant apple (Dillenia indica) juice was investigated. The juice samples were analysed periodically (0, 1, 2, 5, 10, 20, 30, 40, 50 and 60 days) during 60 days of storage period and results were compared with thermally processed as well as with untreated (fresh juice) samples. RESULTS: Slight variations had been observed in the quality parameters like pH, °Brix and total acidity. Other parameters like colour values, antioxidant activity, total phenols and total flavonoids were varied significantly (P < 0.05) in between the treated (HPP and thermal) and untreated juice samples. The microbial counts of the HPP treated samples were lower than the other samples. Sensory results also showed similar results to those of the other analyses that the treated samples were better for consumption rather than the untreated samples. The shelf-life of the HPP processed elephant apple juice was established as 60 days at 4 °C. CONCLUSION: This study showed that application of HPP effectively maintained quality attributes and extended shelf life of the elephant apple juice. It may be suggested that application of HPP could be considered for commercial application during storage and marketing. © 2016 Society of Chemical Industry. [Mh] Termos MeSH primário: Dilleniaceae/química Conservação de Alimentos/métodos Qualidade dos Alimentos Sucos de Frutas e Vegetais/análise Pressão [Mh] Termos MeSH secundário: Contagem de Colônia Microbiana Dilleniaceae/microbiologia Flavonoides/análise Manipulação de Alimentos/métodos Sucos de Frutas e Vegetais/microbiologia Temperatura Alta Concentração de Íons de Hidrogênio Fenóis/análise Esterilização/métodos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Flavonoids); 0 (Phenols) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170906 [Lr] Data última revisão: 170906 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160706 [St] Status: MEDLINE [do] DOI: 10.1002/jsfa.7878

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[PMID]: 27247703 [Au] Autor: Lopes RH; Macorini LF; Antunes KÁ; Espindola PP; Alfredo TM; da Rocha Pdos S; Pereira ZV; Dos Santos EL; de Picoli Souza K [Ad] Endereço: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil. [Ti] Título: Antioxidant and Hypolipidemic Activity of the Hydroethanolic Extract of Curatella americana L. Leaves. [So] Source: Oxid Med Cell Longev;2016:9681425, 2016. [Is] ISSN: 1942-0994 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: High levels of reactive oxygen species in the body and hyperlipidemia are key factors for the development of cardiovascular diseases such as atherosclerosis. The present study investigated the antioxidant and hypolipidemic activity of hydroethanolic extract of Curatella americana L. leaves (ExC). The antioxidant activity of ExC was assessed by 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging capacity and protection against hemolysis induced by 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), followed by quantification of malondialdehyde (MDA). Wistar rats with hyperlipidemia induced by high-fructose diet (60%) were treated for 60 days with water, simvastatin (30 mg·Kg(-1)), ciprofibrate (2 mg·Kg(-1)), and ExC (200 mg·Kg(-1)). ExC revealed IC50 of 6.0 ± 0.5 µg·mL(-1), an intermediary value among positive controls used in the assay of DPPH scavenging capacity. At all concentrations (50 to 125 µg·mL(-1)) and times (60 to 240 min) evaluated, ExC protected erythrocytes against AAPH-induced hemolysis, which was confirmed by lower MDA levels. In vivo tests showed a reduction of 34 and 45%, respectively, in serum concentration of cholesterol and triglycerides in hyperlipidemic rats treated with ExC, a similar effect compared to the reference drugs, simvastatin and ciprofibrate, respectively. Together, the results showed the antioxidant activity of ExC and its ability to improve the serum lipid profile in hyperlipidemic rats. [Mh] Termos MeSH primário: Antioxidantes/química Dilleniaceae/química Hipolipemiantes/química Extratos Vegetais/química [Mh] Termos MeSH secundário: Animais Antioxidantes/farmacologia Dieta da Carga de Carboidratos Dilleniaceae/metabolismo Eritrócitos/citologia Eritrócitos/efeitos dos fármacos Eritrócitos/metabolismo Hemólise/efeitos dos fármacos Hiperlipidemias/tratamento farmacológico Hiperlipidemias/etiologia Hipolipemiantes/uso terapêutico Peroxidação de Lipídeos/efeitos dos fármacos Lipídeos/sangue Malondialdeído/análise Extratos Vegetais/farmacologia Folhas de Planta/química Folhas de Planta/metabolismo Ratos Ratos Wistar Sinvastatina/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Hypolipidemic Agents); 0 (Lipids); 0 (Plant Extracts); 4Y8F71G49Q (Malondialdehyde); AGG2FN16EV (Simvastatin) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170308 [Lr] Data última revisão: 170308 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160602 [St] Status: MEDLINE [do] DOI: 10.1155/2016/9681425

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[PMID]: 27131434 [Au] Autor: Foo JB; Saiful Yazan L; Tor YS; Wibowo A; Ismail N; Armania N; Cheah YK; Abdullah R [Ad] Endereço: Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: foojhibiau@gmail.com. [Ti] Título: Dillenia suffruticosa dichloromethane root extract induced apoptosis towards MDA-MB-231 triple-negative breast cancer cells. [So] Source: J Ethnopharmacol;187:195-204, 2016 Jul 01. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Dillenia suffruticosa is traditionally used for treatment of cancerous growth including breast cancer in Malaysia. AIM OF THE STUDY: Dillenia suffruticosa is a well-known medicinal plant in Malaysia for the treatment of cancer. Nevertheless, no study has been reported the cytotoxicity of this plant towards MDA-MB-231 triple-negative breast cancer cells. The present study was designed to investigate the mode of cell death and signalling pathways of MDA-MB-231 cells treated with dichloromethane Dillenia suffruticosa root extract (DCM-DS). METHODS: Extraction of Dillenia suffruticosa root was performed by the use of sequential solvent procedure. The cytotoxicity of DCM-DS was determined by using MTT assay. The mode of cell death was evaluated by using an inverted light microscope and flow cytometry analysis using Annexin-V/PI. Cell cycle analysis and measurement of reactive oxygen species level were performed by using flow cytometry. The cells were treated with DCM-DS and antioxidants α-tocopherol or ascorbic acid to evaluate the involvement of ROS in the cytotoxicity of DCM-DS. Effect of DCM-DS on the expression of antioxidant, apoptotic, growth, survival genes and proteins were analysed by using GeXP-based multiplex system and Western blot, respectively. The cytotoxicity of compounds isolated from DCM-DS was evaluated towards MDA-MB-231 cells using MTT assay. RESULTS: DCM-DS induced apoptosis, G2/M phase cell cycle arrest and oxidative stress in MDA-MB-231 cells. The induction of apoptosis in MDA-MB-231 cells by DCM-DS is possibly due to the activation of pro-apoptotic JNK1 and down-regulation of anti-apoptotic ERK1, which in turn down-regulates anti-apoptotic BCL-2 to increase the BAX/BCL-2 ratio to initiate the mitochondrial apoptotic pathway. The cell cycle arrest in DCM-DS-treated MDA-MB-231 cells is possibly via p53-independent but p21-dependent pathway. A total of 3 triterpene compounds were isolated from DCM-DS. Betulinic acid appears to be the most major and most cytotoxic compound in DCM-DS. CONCLUSION: The data suggest the potential application of DCM-DS in the treatment of triple-negative breast cancer. [Mh] Termos MeSH primário: Antineoplásicos/farmacologia Dilleniaceae Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos Ciclo Celular/efeitos dos fármacos Linhagem Celular Tumoral Sobrevivência Celular/efeitos dos fármacos Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos Seres Humanos Proteína Quinase 3 Ativada por Mitógeno/genética Proteína Quinase 8 Ativada por Mitógeno/genética Raízes de Plantas Proteínas Proto-Oncogênicas c-bcl-2/genética Neoplasias de Mama Triplo Negativas/tratamento farmacológico Neoplasias de Mama Triplo Negativas/genética Neoplasias de Mama Triplo Negativas/metabolismo Proteína Supressora de Tumor p53/genética Proteína X Associada a bcl-2/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents); 0 (BAX protein, human); 0 (Plant Extracts); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Tumor Suppressor Protein p53); 0 (bcl-2-Associated X Protein); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170302 [Lr] Data última revisão: 170302 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160502 [St] Status: MEDLINE

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MEDLINE

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[PMID]: 26416622 [Au] Autor: Fu C; Yang D; Peh WY; Lai S; Feng X; Yang H [Ad] Endereço: Food Science and Technology Programme, Dept. of Chemistry, National Univ. of Singapore, 3 Science Drive 3, Singapore, 117543, Republic of Singapore. [Ti] Título: Structure and Antioxidant Activities of Proanthocyanidins from Elephant Apple (Dillenia indica Linn.). [So] Source: J Food Sci;80(10):C2191-9, 2015 Oct. [Is] ISSN: 1750-3841 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: UNLABELLED: Proanthocyanidins were isolated and purified from fruits of elephant apple (Dillenia indica Linn.) and their structural and bioactive properties were examined. Bate-Smith alcoholysis, FTIR, and (13) C NMR spectra revealed that elephant apple proanthocyanidins (EAPs) contained a dominant amount of B-type procyanidins (PC) with a minor amount of B-type prodelphinidins (PD) but no A-type interflavan linkage. (13) C NMR spectrum indicated that the cis isomer was dominant in EAPs. The electron spray ionization and matrix-assisted laser desorption ionization time of flight mass spectra of EAPs showed the clear ion peaks corresponding to B-type PC dimer to B-type PD with degree of polymerization of 11. EAPs had strong antioxidant activity, which was evidenced by the high oxygen radical scavenging capacity at 1.06 × 10(4) µmol TE/g and ferric reducing antioxidant power of 2320 µmol Fe(II)/g. The results suggest that EAPs could be extracted to be used as promising functional food materials. PRACTICAL APPLICATION: In this study, the elephant apple proanthocyanidins (EAPs) with a yield of 0.23% were identified for the first time as dominant B-type poly(catechin/epicatechin) but no A-type interflavan linkage. EAPs had higher ORAC and FRAP values compared to commercial grape seed proanthocyanidins, suggesting that EAPs may be used as promising functional food materials. [Mh] Termos MeSH primário: Antioxidantes/farmacologia Biflavonoides/farmacologia Catequina/farmacologia Dilleniaceae/química Frutas/química Extratos Vegetais/farmacologia Proantocianidinas/farmacologia [Mh] Termos MeSH secundário: Animais Antocianinas/análise Antocianinas/química Antocianinas/farmacologia Antioxidantes/análise Antioxidantes/química Biflavonoides/análise Biflavonoides/química Catequina/análise Catequina/química Extrato de Sementes de Uva/farmacologia Estrutura Molecular Oxirredução Extratos Vegetais/química Proantocianidinas/análise Proantocianidinas/química Proantocianidinas/isolamento & purificação Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anthocyanins); 0 (Antioxidants); 0 (Biflavonoids); 0 (Grape Seed Extract); 0 (Grape Seed Proanthocyanidins); 0 (Plant Extracts); 0 (Proanthocyanidins); 15514-06-4 (procyanidin B); 8R1V1STN48 (Catechin) [Em] Mês de entrada: 1605 [Cu] Atualização por classe: 151008 [Lr] Data última revisão: 151008 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150930 [St] Status: MEDLINE [do] DOI: 10.1111/1750-3841.13005

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