Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.325 [Categoria DeCS]
Referências encontradas : 50 [refinar]
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[PMID]:27856932
[Au] Autor:Sun Y; Qiu H; Ou M; Chen R; Liang G
[Ad] Endereço:Department of Pharmacy, Guangxi Medical University, Nanning, Guangxi, China.
[Ti] Título:Taccaoside induces apoptosis in hepatocellular carcinoma cell lines.
[So] Source:J Int Med Res;44(6):1395-1402, 2016 Dec.
[Is] ISSN:1473-2300
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objective Taccaoside, a steroidal saponin, has been shown to be cytotoxic, although the mechanism of cytotoxicity remains unclear. This study examined the effect of taccaoside on the human hepatocellular carcinoma (HCC) cell lines SMMC-7721 and Bel-7404. Methods The antiproliferative effect of taccaoside were measured using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Cells were stained with Hoechst 33258 to observe morphology. Cell cycle and apoptosis were analysed by flow cytometry. Caspase activation was detected using specific assays, and PARP, Bax and Bcl-2 expression were analysed using western blotting. Results Taccaoside showed antiproliferative effect on HCC cell lines growth in a concentration- and time-dependent manner. Taccaoside arrested cell cycle in the G2/M phase and induced caspase-dependent apoptosis. Western blotting indicated that taccaoside upregulated Bax expression and downregulated Bcl-2 expression. PARP cleavage was observed following taccaoside treatment. Conclusions This study showed that taccaoside may inhibit HCC cell proliferation by inducing apoptosis.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Dioscoreaceae/química
Regulação Neoplásica da Expressão Gênica
Hepatócitos/efeitos dos fármacos
Saponinas/farmacologia
Esteroides/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação
Apoptose/genética
Caspase 3/genética
Caspase 3/metabolismo
Caspase 8/genética
Caspase 8/metabolismo
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos
Hepatócitos/metabolismo
Hepatócitos/patologia
Seres Humanos
Extratos Vegetais/química
Poli(ADP-Ribose) Polimerases/genética
Poli(ADP-Ribose) Polimerases/metabolismo
Proteólise/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-bcl-2/antagonistas & inibidores
Proteínas Proto-Oncogênicas c-bcl-2/genética
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Saponinas/isolamento & purificação
Transdução de Sinais
Esteroides/isolamento & purificação
Proteína X Associada a bcl-2/agonistas
Proteína X Associada a bcl-2/genética
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (BAX protein, human); 0 (BCL2 protein, human); 0 (Plant Extracts); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Saponins); 0 (Steroids); 0 (bcl-2-Associated X Protein); 0 (taccaoside C); EC 2.4.2.30 (Poly(ADP-ribose) Polymerases); EC 3.4.22.- (CASP3 protein, human); EC 3.4.22.- (CASP8 protein, human); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 8)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE
[do] DOI:10.1177/0300060516672368


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[PMID]:27821045
[Au] Autor:Maurin O; Muasya AM; Catalan P; Shongwe EZ; Viruel J; Wilkin P; van der Bank M
[Ad] Endereço:African Centre for DNA Barcoding, Department of Botany & Plant Biotechnology, University of Johannesburg, PO Box 524, Auckland Park, Johannesburg, Gauteng, 2006, South Africa.
[Ti] Título:Diversification into novel habitats in the Africa clade of Dioscorea (Dioscoreaceae): erect habit and elephant's foot tubers.
[So] Source:BMC Evol Biol;16(1):238, 2016 Nov 08.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Dioscorea is a widely distributed and highly diversified genus in tropical regions where it is represented by ten main clades, one of which diversified exclusively in Africa. In southern Africa it is characterised by a distinct group of species with a pachycaul or "elephant's foot" structure that is partially to fully exposed above the substrate. In contrast to African representatives of the genus from other clades, occurring mainly in forest or woodland, the pachycaul taxa and their southern African relatives occur in diverse habitats ranging from woodland to open vegetation. Here we investigate patterns of diversification in the African clade, time of transition from forest to more open habitat, and morphological traits associated with each habitat and evaluate if such transitions have led to modification of reproductive organs and mode of dispersal. RESULTS: The Africa clade originated in the Oligocene and comprises four subclades. The Dioscorea buchananii subclade (southeastern tropical Africa and South Africa) is sister to the East African subclade, which is respectively sister to the recently evolved sister South African (e. g., Cape and Pachycaul) subclades. The Cape and Pachycaul subclades diversified in the east of the Cape Peninsula in the mid Miocene, in an area with complex geomorphology and climate, where the fynbos, thicket, succulent karoo and forest biomes meet. CONCLUSIONS: Diversification out of forest is associated with major shifts in morphology of the perennial tuber (specifically an increase in size and orientation which presumably led them to become pachycaul) and rotation of stem (from twining to non-twining). The iconic elephant's foot morphology, observed in grasslands and thicket biomes, where its corky bark may offer protection against fire and herbivory, evolved since mid Miocene. A shift in pollination trait is observed within the forest, but entry into open habitat does not show association with reproductive morphology, except in the seed wing, which has switched from winged all round the seed margin to just at the base or at the apex of it, or has been even replaced by an elaiosome.
[Mh] Termos MeSH primário: Dioscorea/anatomia & histologia
Dioscoreaceae/anatomia & histologia
Ecossistema
Folhas de Planta/anatomia & histologia
[Mh] Termos MeSH secundário: África
Animais
Clima
Dioscorea/classificação
Dioscorea/fisiologia
Dioscoreaceae/classificação
Dioscoreaceae/fisiologia
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE


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[PMID]:27335389
[Au] Autor:Lim GS; Barrett CF; Pang CC; Davis JI
[Ad] Endereço:The New York Botanical Garden, Pfizer Plant Research Laboratory, 2900 Southern Boulevard, Bronx, New York 10458 USA L. H. Bailey Hortorium, Section of Plant Biology, 412 Mann Library Building, Cornell University, Ithaca, New York 14853 USA gsl47@cornell.edu.
[Ti] Título:Drastic reduction of plastome size in the mycoheterotrophic Thismia tentaculata relative to that of its autotrophic relative Tacca chantrieri.
[So] Source:Am J Bot;103(6):1129-37, 2016 06.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Heterotrophic angiosperms tend to have reduced plastome sizes relative to those of their autotrophic relatives because genes that code for proteins involved in photosynthesis are lost. However, some plastid-encoded proteins may have vital nonphotosynthetic functions, and the plastome therefore may be retained after the loss of photosynthesis. METHODS: We sequenced the plastome of the mycoheterotrophic species Thismia tentaculata and a representative of its sister genus, Tacca chantrieri, using next-generation technology, and we compared sequences and structures of genes and genomes of these species. KEY RESULTS: The plastome of Tacca chantrieri is similar to those of other autotrophic taxa of Dioscoreaceae, except in a few local rearrangements and one gene loss. The plastome of Thismia tentaculata is ca. 16 kbp long with a quadripartite structure and is among the smallest known plastomes. Synteny is minimal between the plastomes of Tacca chantrieri and Thismia tentaculata. The latter includes only 12 candidate genes, with all except accD involved in protein synthesis. Of the 12 genes, trnE, trnfM, and accD are frequently among the few that remain in depauperate plastomes. CONCLUSIONS: The plastome of Thismia tentaculata, like those of most other heterotrophic plants, includes a small number of genes previously suggested to be essential to plastome survival.
[Mh] Termos MeSH primário: Processos Autotróficos/genética
Dioscoreaceae/genética
Tamanho do Genoma
Genomas de Plastídeos
Processos Heterotróficos/genética
Magnoliopsida/genética
[Mh] Termos MeSH secundário: Flores/anatomia & histologia
Genes de Plantas
Estudos de Associação Genética
Hong Kong
Nucleotídeos/genética
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Nucleotides)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160624
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600042


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[PMID]:27313159
[Au] Autor:Dike VT; Vihiior B; Bosha JA; Yin TM; Ebiloma GU; de Koning HP; Igoli JO; Gray AI
[Ad] Endereço:Phytochemistry Research Group, Department of Chemistry, University of Agriculture PMB 2373, Makurdi, Benue State, Nigeria.
[Ti] Título:Antitrypanosomal Activity of a Novel Taccalonolide from the Tubers of Tacca leontopetaloides.
[So] Source:Phytochem Anal;27(3-4):217-21, 2016 May.
[Is] ISSN:1099-1565
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Several taccalonolides with various bioactivities have been isolated from Tacca species but no studies to isolate taccalonolides with anti-trypanosomal activity from Tacca leontopetaloides have been reported. OBJECTIVES: To analyse extracts of the roots of Tacca leontopetaloides, purify the extracts by column chromatography and identify isolated compounds by spectroscopic methods. The compounds and fractions will be tested for antitrypanosomal activity in vitro against Trypanosoma brucei brucei. MATERIAL AND METHODS: Dried roots or tubers of Tacca leontopetaloides, chromatographic separation and spectroscopic identification. RESULTS: A novel taccalonolide A propanoate and some known taccalonolides were isolated and their structures were determined by NMR and mass spectrometry CONCLUSION: Several taccalonolides were isolated from Tacca leontopetaloides and were found to have in vitro antitrypanosomal activity against Trypanosoma brucei brucei and EC50 values for the isolated compounds were from 0.79 µg/mL. Copyright © 2016 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Dioscoreaceae/química
Extratos Vegetais/farmacologia
Esteroides/farmacologia
Tripanossomicidas/farmacologia
Trypanosoma brucei brucei/efeitos dos fármacos
[Mh] Termos MeSH secundário: Espectrometria de Massas
Estrutura Molecular
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Raízes de Plantas/química
Tubérculos/química
Propionatos/química
Propionatos/isolamento & purificação
Propionatos/farmacologia
Esteroides/química
Esteroides/isolamento & purificação
Tripanossomicidas/química
Tripanossomicidas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Propionates); 0 (Steroids); 0 (Trypanocidal Agents); 0 (taccalonolide A)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160618
[St] Status:MEDLINE
[do] DOI:10.1002/pca.2619


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[PMID]:27287369
[Au] Autor:Yen PH; Chi VT; Kiem PV; Tai BH; Quang TH; Nhiem NX; Anh Hle T; Ban NK; Thanh BV; Minh CV; Park S; Kim SH
[Ad] Endereço:Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
[Ti] Título:Spirostanol saponins from Tacca vietnamensis and their anti-inflammatory activity.
[So] Source:Bioorg Med Chem Lett;26(15):3780-4, 2016 08 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Using various chromatographic methods, five new steroidal saponins named taccavietnamosides A-E (1-5) and three known, (24S,25R)-spirost-5-en-3ß,24-diol 3-O-α-l-rhamnopyranosyl-(1→2)-[α-l-rhamnopyranosyl-(1→3)]-ß-d-glucopyranoside (6), (24S,25R)-spirost-5-en-3ß,24-diol 3-O-α-l-rhamnopyranosyl-(1→2)-[ß-d-glucopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→3)]-ß-d-glucopyranoside (7), and chantrieroside A (8) were isolated from the rhizomes of Tacca vietnamensis Thin et Hoat. Their chemical structures were elucidated by physical and chemical methods. All compounds were evaluated for the inhibitory activities of nitric oxide production in LPS-stimulated RAW 264.7 macrophages and BV2 cells. As the results, compounds 3-5 showed moderate inhibition on NO production in LPS-stimulated BV2 cells and RAW 264.7 macrophages with the IC50 values ranging from 37.0 to 60.7µM.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/farmacologia
Dioscoreaceae/química
Óxido Nítrico/antagonistas & inibidores
Saponinas/farmacologia
Espirostanos/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/química
Anti-Inflamatórios não Esteroides/isolamento & purificação
Linhagem Celular
Relação Dose-Resposta a Droga
Lipopolissacarídeos/antagonistas & inibidores
Lipopolissacarídeos/farmacologia
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
Camundongos
Estrutura Molecular
Óxido Nítrico/biossíntese
Células RAW 264.7
Saponinas/química
Saponinas/isolamento & purificação
Espirostanos/química
Espirostanos/isolamento & purificação
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Lipopolysaccharides); 0 (Saponins); 0 (Spirostans); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE


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[PMID]:26996017
[Au] Autor:Yen PH; Chi VT; Kim DC; Ko W; Oh H; Kim YC; Dung DT; Thanh NT; Quang TH; Ngan NT; Nhiema NX; Anh Hle T; Minh CV; Kiem PV
[Ti] Título:Steroidal Glucosides from the Rhizomes of Tacca chantrieri and Their Inhibitory Activities of NO Production in BV2 Cells.
[So] Source:Nat Prod Commun;11(1):45-8, 2016 Jan.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two new steroidal glucosides, chantriolides D and E (1 and 2), along with four known compounds, chantriolide A (3), chantriolide B (4), chantriolide C (5), and (25S)-spirost-5-en-3-ol 3-O-α-L-rhamnopyranosyl-(1-->2)-O-[α-L-rhamnopyranosyl-(1-->3)]-ß-D-glucopyranoside (6) were isolated from the rhizomes of Tacca chantrieri. Their structures were determined by 1D and 2D NMR spectroscopic and HR-ESI-MS data, as well as by comparison with reported data. Compounds 1 and 2 were found to show strong inhibitory NO effect in BV2 cells, with IC50 values of 12.45 and 59.03 µM, respectively.
[Mh] Termos MeSH primário: Dioscoreaceae/química
Glucosídeos/farmacologia
Microglia/efeitos dos fármacos
Óxido Nítrico/biossíntese
Rizoma/química
[Mh] Termos MeSH secundário: Linhagem Celular
Glucosídeos/química
Seres Humanos
Microglia/metabolismo
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glucosides); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160321
[Lr] Data última revisão:
160321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160322
[St] Status:MEDLINE


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[PMID]:26718088
[Au] Autor:Liu ZH; Yan H; Si YA; Ni W; Chen Y; He L; Liu HY
[Ad] Endereço:State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Bioactive spirostane glycosides from Tacca plantaginea.
[So] Source:Steroids;106:86-92, 2016 Feb.
[Is] ISSN:1878-5867
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eight new spirostane glycosides, taccaosides E-L (2-7, 11, and 14), along with 7 known spirostane glycosides were isolated from the extract of the whole plants of Tacca plantaginea (Hance) Drenth. Their structures were established on the basis of physical data, spectroscopic analysis (MS, 1D and 2D NMR), and chemical methods. The cytotoxicities of the isolates were evaluated in vitro against two human cancer cell lines (HEK293 and HepG2). The results showed that compound 1 had the strongest cytotoxic activity with IC50 values of 1.7 µM and 1.2 µM against the two cancer cell lines, respectively. Furthermore, the spirostane glycosides with 17α-hydroxyl group at their aglycones (10, 12, and 15) were tested for the induced platelet aggregation activity.
[Mh] Termos MeSH primário: Dioscoreaceae/química
Glicosídeos/química
Glicosídeos/farmacologia
Espirostanos/química
[Mh] Termos MeSH secundário: Glicosídeos/isolamento & purificação
Células HEK293
Células Hep G2
Seres Humanos
Agregação Plaquetária/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glycosides); 0 (Spirostans)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160101
[St] Status:MEDLINE


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[PMID]:26363199
[Au] Autor:Jiang W; Luo F; Lu Q; Liu J; Li P; Wang X; Fu Y; Hao K; Yan T; Ding X
[Ad] Endereço:State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
[Ti] Título:The protective effect of Trillin LPS-induced acute lung injury by the regulations of inflammation and oxidative state.
[So] Source:Chem Biol Interact;243:127-34, 2016 Jan 05.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Inflammation response and oxidative stress have been reported to be involved in the pathogenesis of acute lung injury (ALI). Accordingly, anti-inflammatory treatment is proposed to be a possible efficient therapeutic strategy for ALI. The purpose of our present study was to evaluate the anti-inflammatory efficacy of trillin (Tr) on ALI induced by lipopolysaccharide (LPS) in mice and explore the underlying mechanism. BALB/c mice received Tr (50, 100 mg/kg) intraperitoneally 1 h prior to the intratracheal instillation of lipopolysaccharide (LPS) challenge. Pretreatment with Tr at the dose of 50, 100 mg/kg markedly ameliorated lung wet-to-dry weight (W/D) ratio, myeloperoxidase (MPO) activity and pulmonary histopathological conditions. In addition, the protective efficacy of Tr might be attributed to the down-regulations of neutrophil infiltration, malondialdehyde (MDA), inflammatory cytokines and the up-regulations of super-oxide dismutase (SOD), catalase(CAT), glutathione(GSH), Glutathione Peroxidase(GSH-Px) in bronchoalveolar lavage fluid (BALF). Meanwhile, our study revealed some correlations between (NF-E2-related factor 2) Nrf2/heme oxygenase (HO)-1/nuclear factor-kappa B (NF-κB) pathways and the beneficial effect of Tr, as evidenced by the significant up-regulations of HO-1 and Nrf2 protein expressions as well as the down-regulations of p-NF-κB and p-inhibitor of NF-κB (IκB) in lung tissues. Taken together, our results indicated that Tr exhibited protective effect on LPS-induced ALI by the regulations of related inflammatory events via the activations of Nrf2, HO-1 and NF-κB pathway. The current study indicated that Tr could be a potentially effective candidate medicine for the treatment of ALI.
[Mh] Termos MeSH primário: Lesão Pulmonar Aguda/tratamento farmacológico
Anti-Inflamatórios/uso terapêutico
Antioxidantes/uso terapêutico
Inflamação/tratamento farmacológico
Pulmão/efeitos dos fármacos
Saponinas/uso terapêutico
[Mh] Termos MeSH secundário: Lesão Pulmonar Aguda/complicações
Lesão Pulmonar Aguda/imunologia
Lesão Pulmonar Aguda/patologia
Animais
Anti-Inflamatórios/química
Antioxidantes/química
Citocinas/imunologia
Dioscoreaceae/química
Heme Oxigenase-1/imunologia
Inflamação/complicações
Inflamação/imunologia
Inflamação/patologia
Lipopolissacarídeos/imunologia
Pulmão/imunologia
Pulmão/patologia
Masculino
Camundongos Endogâmicos BALB C
Fator 2 Relacionado a NF-E2/imunologia
NF-kappa B/imunologia
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/química
Saponinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Cytokines); 0 (Lipopolysaccharides); 0 (NF-E2-Related Factor 2); 0 (NF-kappa B); 0 (Plant Extracts); 0 (Saponins); EC 1.14.14.18 (Heme Oxygenase-1)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150913
[St] Status:MEDLINE


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[PMID]:26344383
[Au] Autor:Guo L; Zeng SL; Zhang Y; Li P; Liu EH
[Ad] Endereço:State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
[Ti] Título:Comparative analysis of steroidal saponins in four Dioscoreae herbs by high performance liquid chromatography coupled with mass spectrometry.
[So] Source:J Pharm Biomed Anal;117:91-8, 2016 Jan 05.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Steroidal saponins, which exhibit multiple pharmacological effects, are the major bioactive constituents in herbal medicines from Dioscoreae species. In this study, a sensitive method based on high performance liquid chromatography-mass spectrometry (HPLC-MS) was established and validated for qualitative and quantitative analysis of steroidal saponins in four Dioscoreae herbs including Dioscoreae Nipponica Rhizome (DNR) and Dioscoreae Hypoglaucae Rhizome (DHR), Dioscoreae Spongiosae Rhizome (DSR) and Dioscoreae Rhizome (DR). A total of eleven steroidal saponins were identified by high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF/MS). Furthermore, seven major steroidal saponins was simultaneous quantified using a high performance liquid chromatography coupled with triple quadrupole mass spectrometry (HPLC-QQQ/MS). The qualitative and quantitative analysis results indicated that the chemical composition of DNR, DHR and DSR samples exhibited a high level of global similarity, while the ingredients in DR varied greatly from the other three herbs. Moreover, principal component analysis (PCA) and hierarchical clustering analysis (HCA) were performed to compare and discriminate the Dioscoreae herbs based on the quantitative data. The results demonstrated the qualitative and quantitative analysis of steroidal saponins based on HPLC-MS is a feasible method for quality control of Dioscoreae herbs.
[Mh] Termos MeSH primário: Dioscoreaceae
Fitosteróis/análise
Preparações de Plantas/análise
Saponinas/análise
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Phytosterols); 0 (Plant Preparations); 0 (Saponins)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151107
[Lr] Data última revisão:
151107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150908
[St] Status:MEDLINE


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[PMID]:26423865
[Au] Autor:Yokosuka A
[Ad] Endereço:Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences.
[Ti] Título:[Discovery of Novel Biologically Active Compounds of Natural Origin, with a Focus on Anti-tumor Activity].
[So] Source:Yakugaku Zasshi;135(10):1109-14, 2015.
[Is] ISSN:1347-5231
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Numerous clinically valuable medicines, including anticancer drugs, have been developed from biologically active natural compounds and their structurally related derivatives. This review discusses novel natural compounds with promising biological activities and those with novel chemical structures. Glaziovianin A, an isoflavone isolated from the leaves of Ateleia glazioviana (Legminosae), inhibited cell cycle progression at the M-phase with an abnormal spindle structure. AU-1 and YG-1, 5ß-steroidal glycosides isolated from the whole plants of Agave utahensis and the underground parts of Yucca glauca (Agavaceae), induced apoptosis of HL-60 cells via caspase-3 activation. Lycolicidinol, an alkaloid isolated from the bulbs of Lycoris albiflora (Amaryllidaceae), induced transient autophagy and morphological changes in mitochondria in the early stage of the apoptotic cell death process in HSC-2 cells. Taccasterosides isolated from the rhizomes of Tacca chantrieri (Taccaceae) and stryphnosides isolated from the pericarps of Stryphnodendron fissuratum (Legminosae) are steroidal and triterpene glycosides with unique chemical structures having novel sugar sequences.
[Mh] Termos MeSH primário: Agave/química
Alcaloides/isolamento & purificação
Alcaloides/farmacologia
Antineoplásicos
Descoberta de Drogas
Fabaceae/química
Glicosídeos/isolamento & purificação
Glicosídeos/farmacologia
Isoflavonas/isolamento & purificação
Isoflavonas/farmacologia
Lycoris/química
Yucca/química
[Mh] Termos MeSH secundário: Ampicilina/análogos & derivados
Ampicilina/química
Ampicilina/isolamento & purificação
Ampicilina/farmacologia
Animais
Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Caspase 3/metabolismo
Ciclo Celular/efeitos dos fármacos
Divisão Celular/efeitos dos fármacos
Dioscoreaceae/química
Glicosídeos/química
Células HL-60
Seres Humanos
Isoflavonas/química
Mitocôndrias/efeitos dos fármacos
Conformação Molecular
Fitoterapia
Folhas de Planta
Ratos
Fuso Acromático/efeitos dos fármacos
Relação Estrutura-Atividade
Triterpenos/isolamento & purificação
Triterpenos/farmacologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Alkaloids); 0 (Antineoplastic Agents); 0 (Glycosides); 0 (Isoflavones); 0 (N-(6,7-difluoroquinolonyl)ampicillin); 0 (Triterpenes); 0 (glaziovianin A); 7C782967RD (Ampicillin); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151001
[Lr] Data última revisão:
151001
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151002
[St] Status:MEDLINE
[do] DOI:10.1248/yakushi.15-00185



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