[PMID]: | 25702282 |
[Au] Autor: | Panusa A; Petrucci R; Marrosu G; Multari G; Gallo FR |
[Ad] Endereço: | Dipartimento del Farmaco, Istituto Superiore di Sanità, Viale Regina Elena 299, I-00161 Rome, Italy. Electronic address: alessia.panusa@iss.it. |
[Ti] Título: | UHPLC-PDA-ESI-TOF/MS metabolic profiling of Arctostaphylos pungens and Arctostaphylos uva-ursi. A comparative study of phenolic compounds from leaf methanolic extracts. |
[So] Source: | Phytochemistry;115:79-88, 2015 Jul. |
[Is] ISSN: | 1873-3700 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | The aim of this study was to get a rapid metabolic fingerprinting and to gain insight into the metabolic profiling of Arctostaphylos pungens H. B. K., a plant morphologically similar to Arctostaphylos uva-ursi (L.) Spreng. (bearberry) but with a lower arbutin (Arb) content. According to the European Pharmacopoeia the Arb content in the dried leaf of A. uva-ursi (L.) Spreng. must be at least 7% (wt/wt) but other species, like A. pungens, are unintentionally or fraudulently marketed instead of it. Therefore, methanolic leaf extracts of nine A. uva-ursi and six A. pungens samples labeled and marketed as "bearberry leaf" have been analyzed. A five-minute gradient with a UHPLC-PDA-ESI-TOF/MS on an Acquity BEH C18 (50×2.1 mm i.d.) 1.7 µm analytical column has been used for the purpose. A comprehensive assignment of secondary metabolites has been carried out in a comparative study of the two species. Among twenty-nine standards of natural compounds analyzed, fourteen have been identified, while other fifty-five metabolites have been tentatively assigned. Moreover, differences in both metabolic fingerprinting and profiling have been evidenced by statistical multivariate analysis. Specifically, main variations have been observed in the relative content for Arb, as expected, and for some galloyl derivative like tetra- and pentagalloylglucose more abundant in A. uva-ursi than in A. pungens. Furthermore, differences in flavonols profile, especially in myricetin and quercetin glycosilated derivatives, were observed. Based on principal component analysis myricetrin, together with a galloyl arbutin isomer and a disaccharide are herein proposed as distinctive metabolites for A. pungens. |
[Mh] Termos MeSH primário: |
Arctostaphylos
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[Mh] Termos MeSH secundário: |
Arbutina/análise Arctostaphylos/química Arctostaphylos/genética Arctostaphylos/metabolismo Ericaceae/química Flavonoides/análise Taninos Hidrolisáveis/análise Metabolômica Metanol Ressonância Magnética Nuclear Biomolecular Fenóis/análise Extratos Vegetais/química Folhas de Planta/química Quercetina/análise
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Flavonoids); 0 (Hydrolyzable Tannins); 0 (Phenols); 0 (Plant Extracts); 76XC01FTOJ (myricetin); 9IKM0I5T1E (Quercetin); C5INA23HXF (Arbutin); Y4S76JWI15 (Methanol) |
[Em] Mês de entrada: | 1604 |
[Cu] Atualização por classe: | 150609 |
[Lr] Data última revisão:
| 150609 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 150223 |
[St] Status: | MEDLINE |
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