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Pesquisa : B01.650.940.800.575.912.250.341.937.437 [Categoria DeCS]
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  1 / 31 MEDLINE  
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[PMID]:27630118
[Au] Autor:Shutoh K; Kaneko S; Suetsugu K; Naito YI; Kurosawa T
[Ad] Endereço:Graduate School of Symbiotic Systems Science and Technology, Fukushima University, 1 Kanayagawa, Fukushima 960-1296, Japan kohshutoh@gmail.com.
[Ti] Título:Variation in vegetative morphology tracks the complex genetic diversification of the mycoheterotrophic species Pyrola japonica sensu lato.
[So] Source:Am J Bot;103(9):1618-29, 2016 09.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Although the evolution of full mycoheterotrophy has attracted many plant researchers, molecular phylogenetic studies that focus on the transition from partial to full mycoheterotrophy are limited to a few taxa. Pyrola japonica sensu lato is an ideal model for examining the evolution of mycoheterotrophy, owing to its variable leaf size, which suggests that the species comprises several transitional stages. METHODS: To elucidate the molecular and morphological changes that occur during the evolutionary transition between partial and full mycoheterotrophy in P. japonica s.l. from 18 populations in Japan, we estimated a parsimony network of plastid haplotypes based on three noncoding regions, measured the leaf size and scape color of the shoots, and compared morphology among haplotypes. KEY RESULTS: The seven haplotypes exhibited star-like relationships, and at least three divergent haplotypes were associated with differences in morphology. The first was mainly observed in large-leaved and green-scaped populations, whereas the second was observed in extremely small-leaved and reddish-scaped populations, which indicated a high degree of mycoheterotrophy, and the last was detected among mixed populations with both green- and reddish-scaped shoots with intermediate leaf sizes. In addition, the inconsistent association between the haplotypes and morphology suggests a complex relationship. CONCLUSIONS: Pyrola japonica s.l. has at least three separate genetic lineages that have different leaf morphologies. The genetic lineages and their coexistence could have led to the variable leaf size and suggest the possibility that gene flow from partial to full mycoheterotrophs could reverse the evolutionary transition to full mycoheterotrophy.
[Mh] Termos MeSH primário: Evolução Biológica
Variação Genética
Pyrola/fisiologia
[Mh] Termos MeSH secundário: Haplótipos
Micorrizas/fisiologia
Pyrola/genética
Pyrola/microbiologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600091


  2 / 31 MEDLINE  
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[PMID]:27323714
[Au] Autor:Uesugi T; Nakano M; Selosse MA; Obase K; Matsuda Y
[Ad] Endereço:Laboratory of Forest Mycology, Graduate School of Bioresources, Mie University, Kurimamachiya 1577, Tsu, Mie, 514-8507, Japan.
[Ti] Título:Pyrola japonica, a partially mycoheterotrophic Ericaceae, has mycorrhizal preference for russulacean fungi in central Japan.
[So] Source:Mycorrhiza;26(8):819-829, 2016 Nov.
[Is] ISSN:1432-1890
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Mycorrhizal symbiosis often displays low specificity, except for mycoheterotrophic plants that obtain carbon from their mycorrhizal fungi and often have higher specificity to certain fungal taxa. Partially mycoheterotrophic (or mixotrophic, MX) plant species tend to have a larger diversity of fungal partners, e.g., in the genus Pyrola (Monotropoideae, Ericaceae). Preliminary evidence however showed that the Japanese Pyrola japonica has preference for russulacean fungi based on direct sequencing of the fungal internal transcribed spacer (ITS) region from a single site. The present study challenges this conclusion using (1) sampling of P. japonica in different Japanese regions and forest types and (2) fungal identification by ITS cloning. Plants were sampled from eight sites in three regions, in one of which the fungal community on tree ectomycorrhizal (ECM) tips surrounding P. japonica was also analyzed. In all, 1512 clone sequences were obtained successfully from 35 P. japonica plants and 137 sequences from ECM communities. These sequences were collectively divided into 74 molecular operational taxonomic units (MOTUs) (51 and 33 MOTUs, respectively). MOTUs from P. japonica involved 36 ECM taxa (96 % of all clones), and 17 of these were Russula spp. (76.2 % of all clones), which colonized 33 of the 35 sampled plants. The MOTU composition significantly differed between P. japonica and ECM tips, although shared species represented 26.3 % of the ECM tips community in abundance. This suggests that P. japonica has a preference for russulacean fungi.
[Mh] Termos MeSH primário: Basidiomycota/classificação
Micorrizas/classificação
Pyrola/microbiologia
[Mh] Termos MeSH secundário: Basidiomycota/fisiologia
Clonagem Molecular
Japão
Micorrizas/fisiologia
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160622
[St] Status:MEDLINE


  3 / 31 MEDLINE  
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[PMID]:26369596
[Au] Autor:Kirillov V; Stikhareva T; Atazhanova G; Serafimovich M; Mukanov B; Adekenov S; Mukasheva F; Yrymgali M
[Ad] Endereço:Kazakh Research Institute of Forestry and Agroforestry, Shchuchinsk.
[Ti] Título:Chemical Composition of the Essential Oil of the Boreal Relict of Pyrola rotundifolia L. from Northern Kazakhstan.
[So] Source:J Oleo Sci;64(10):1065-73, 2015.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:In Kazakhstan Pyrola rotundifolia L. is the plant-relict in the flora of insular pine forests of the region of low hillocks and declivities in Kazakhstan - a group of insular pine forests of Kokshetau, Bayanaul and Karkaralinsk. In this study, the essential oils from dried aerial parts of P. rotundifolia, collected in natural habitats of the State National Natural Park "Burabay" (Akmola oblast, Northern Kazakhstan), were extracted by hydrodistillation and analyzed by gas chromatography - mass spectrometry. The yield of the essential oil amounted to 0.057 % in relation to the mass of the air-dry raw material. The major components in dried plant oil were 2,6-dimethyl-1,4-naphthoquinone (12.99-93.49%) and dibutyl phthalate (4.42-40.48%), depending on the growth conditions.
[Mh] Termos MeSH primário: Dibutilftalato/isolamento & purificação
Naftoquinonas/isolamento & purificação
Óleos Voláteis/isolamento & purificação
Óleos Vegetais/isolamento & purificação
Pyrola/química
[Mh] Termos MeSH secundário: Dibutilftalato/química
Cromatografia Gasosa-Espectrometria de Massas
Cazaquistão
Óleos Voláteis/química
Pyrola/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2,6-dimethyl-1,4-naphthoquinone); 0 (Naphthoquinones); 0 (Oils, Volatile); 0 (Plant Oils); 2286E5R2KE (Dibutyl Phthalate)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:151007
[Lr] Data última revisão:
151007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150916
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess15110


  4 / 31 MEDLINE  
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[PMID]:26256648
[Au] Autor:Zhang DY; Yao XH; Duan MH; Luo M; Zhao CJ; Zu YG; Fu YJ
[Ad] Endereço:College of Biotechonlogy and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China.
[Ti] Título:An effective homogenate-assisted negative pressure cavitation extraction for the determination of phenolic compounds in pyrola by LC-MS/MS and the evaluation of its antioxidant activity.
[So] Source:Food Funct;6(10):3323-33, 2015 Oct.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel extraction method, homogenate-assisted negative pressure cavitation extraction (HNPCE), was designed for the extraction and determination of the main phenolic compounds of Pyrola incarnata Fisch. by LC-MS/MS. The particle sizes and extraction yields in the process of homogenization were compared with conventional pulverization. The results showed that homogenization for less than 120 s could produce more suitable particle size powders for analyte extraction. The following NPCE parameters were optimized by a BBD test and under the optimal conditions, the maximum extraction yields of arbutin, epicatechin, hyperin, 2'-O-galloylhyperin and chimaphilin increased by 68.7%, 72.0%, 43.3%, 62.5% and 34.5% with respect to normal NPCE. The LC-MS/MS method was successfully applied for the quantification of five target compounds in pyrola, and the results of the precision test indicated a high accuracy of the present method for the quantification of the target compounds in pyrola. Furthermore, the antioxidant activities of the pyrola extracts were also determined. The results showed that pyrola had good antioxidant activities and it was a valuable antioxidant natural source.
[Mh] Termos MeSH primário: Cromatografia Líquida
Fenóis/análise
Extratos Vegetais/análise
Pyrola/química
Espectrometria de Massas em Tandem
[Mh] Termos MeSH secundário: Antioxidantes/análise
Arbutina/análise
Catequina/análise
Ácido Gálico/análogos & derivados
Ácido Gálico/análise
Naftoquinonas/análise
Quercetina/análogos & derivados
Quercetina/análise
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Naphthoquinones); 0 (Phenols); 0 (Plant Extracts); 53209-27-1 (2''-galloylhyperin); 632XD903SP (Gallic Acid); 8O1CR18L82 (hyperoside); 8R1V1STN48 (Catechin); 9IKM0I5T1E (Quercetin); C5INA23HXF (Arbutin); U8Z086618U (chimaphilin)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151009
[Lr] Data última revisão:
151009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150811
[St] Status:MEDLINE
[do] DOI:10.1039/c5fo00727e


  5 / 31 MEDLINE  
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[PMID]:25236226
[Au] Autor:Yao XH; Zhang DY; Luo M; Jin S; Zu YG; Efferth T; Fu YJ
[Ad] Endereço:State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
[Ti] Título:Negative pressure cavitation-microwave assisted preparation of extract of Pyrola incarnata Fisch. rich in hyperin, 2'-O-galloylhyperin and chimaphilin and evaluation of its antioxidant activity.
[So] Source:Food Chem;169:270-6, 2015 Feb 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel and effective extraction method, namely negative pressure cavitation-microwave assisted extraction technique (NMAE), was developed for the preparation of extracts of Pyrola incarnata Fisch., which are rich in the main constituents hyperin, 2'-O-galloylhyperin and chimaphilin. Single factor experiments and Box-Behnken design (BBD) were combined with a response surface methodology to examine factors affecting extraction. Maximum extraction yields of hyperin, 2'-O-galloylhyperin and chimaphilin (1.339±0.029, 4.831±0.117 and 0.329±0.011mg/g, respectively) were achieved under the following optimised conditions: 700W microwave power, 50°C extraction temperature, 30:1mL/g liquid-solid ratio, -0.05MPa negative pressure, 55% ethanol concentration and 12min extraction time. First-order kinetics equation demonstrated that NMAE offered significant savings in extraction time, and enhancing extraction efficiency. Furthermore, NMAE extracts yielded excellent antioxidant activity (IC50 0.121mg/mL for DPPH 2.896mmol FeSO4/g DW FRAP).
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Ácido Gálico/análogos & derivados
Naftoquinonas/análise
Extratos Vegetais/farmacologia
Pyrola/química
Quercetina/análogos & derivados
[Mh] Termos MeSH secundário: Ácido Gálico/análise
Micro-Ondas
Pressão
Quercetina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Naphthoquinones); 0 (Plant Extracts); 53209-27-1 (2''-galloylhyperin); 632XD903SP (Gallic Acid); 8O1CR18L82 (hyperoside); 9IKM0I5T1E (Quercetin); U8Z086618U (chimaphilin)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:140919
[Lr] Data última revisão:
140919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140920
[St] Status:MEDLINE


  6 / 31 MEDLINE  
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[PMID]:25326138
[Au] Autor:Liu ZW; Jolles DD; Zhou J; Peng H; Milne RI
[Ad] Endereço:Key Laboratory for Plant Diversity and Biogeography of East Asia, Kuming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China zhenwen1980@hotmail.com.
[Ti] Título:Multiple origins of circumboreal taxa in Pyrola (Ericaceae), a group with a Tertiary relict distribution.
[So] Source:Ann Bot;114(8):1701-9, 2014 Dec.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: In the Northern Hemisphere, Tertiary relict disjunctions involve older groups of warm affinity and wide disjunctions, whereas circumboreal distributions in Arctic-Alpine taxa tend to be younger. Arctic-Alpine species are occasionally derived from Tertiary relict groups, but Pyrola species, in particular, are exceptional and they might have occurred multiple times. The aim of this study was to reconstruct the biogeographic history of Pyrola based on a clear phylogenetic analysis and to explore how the genus attained its circumboreal distribution. METHODS: Estimates of divergence times and ancestral geographical distributions based on neutrally evolving DNA sequence variation were used to develop a spatio-temporal model of colonization patterns for Pyrola. KEY RESULTS: Pyrola originated and most diversification occurred in Asia; North America was reached first by series Scotophyllae in the late Miocene, then by sub-clades of series Pyrola and Ellipticae around the Pliocene. The three circumboreal taxa, P. minor, P. chlorantha and the P. rotundifolia complex, originated independently of one another, with the last two originating in Asia. CONCLUSIONS: Three circumboreal Pyrola lineages have arisen independently and at least two of these appear to have originated in Asia. The cool, high-altitude habitats of many Pyrola species and the fact that diversification in the genus coincided with global cooling from the late Miocene onwards fits a hypothesis of pre-adaptation to become circumboreal within this group.
[Mh] Termos MeSH primário: Evolução Biológica
Pyrola/fisiologia
[Mh] Termos MeSH secundário: Sequência de Bases
Calibragem
Filogenia
Filogeografia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1507
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141019
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcu198


  7 / 31 MEDLINE  
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[PMID]:24796694
[Au] Autor:Wang D; He F; Lv Z; Li D
[Ad] Endereço:College of Forestry, Northwest A & F University, Yangling, Shaanxi, China.
[Ti] Título:Phytochemical composition, antioxidant activity and HPLC fingerprinting profiles of three Pyrola species from different regions.
[So] Source:PLoS One;9(5):e96329, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The present study was performed to investigate the variation of phytochemical composition, antioxidant activity and High Performance Liquid Chromatography (HPLC) fingerprinting profiles of three Pyrola species. Thirteen samples (eight P. decorata, three P. calliantha and two P. renifolia) were collected from different regions in China. The tannin, hyperoside and quercetin contents of all samples were determined by reverse-phase HPLC and varied within the range 9.77-34.75, 0.34-2.16 and 0.062-0.147 mg/g dry weigh, respectively. Total flavonoid content was evaluated and varied within the range 16.22-37.82 mg/g dry weight. Antioxidant activity was determined by DPPH assay, with IC50 ranging from 7.96 to 50.33 µg/ml, ABTS•+ and FRAP assay, within the range 612.66-1021.05 and 219.64-398.12 µmol equiv. Trolox/g, respectively. These results revealed that there were significant variations in phytochemical profiles and antioxidant activity among all samples. Due to the higher phytochemical content and significant antioxidant activity, P. calliantha was selected as the most valuable species, and the P. calliantha sample from Left banner of Alxa even possessed the strongest antioxidant activity among all the thirteen samples. Futhermore, Emei Mountain was proved to be the most suitable region for producing P. decorata. Moreover, in order to further evaluate the diversities and quality of Pyrola, HPLC fingerprint analysis coupled with hierarchical cluster and discrimination analyses were introduced to establish a simple, rapid and effective method for accurate identification, classification and quality assessment of Pyrola. Thirteen samples were divided into three groups consistent with their morphological classification. Two types of discriminant functions were generated and the ratio of discrimination was 100%. This method can identify different species of Pyrola and the same species from different regions of origin. Also, it can be used to compare and control the quality of Pyrola and other natural products prepared from them.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Flavonoides/metabolismo
Pyrola/química
[Mh] Termos MeSH secundário: Antioxidantes/química
China
Cromatografia Líquida de Alta Pressão
Medicamentos de Ervas Chinesas/química
Flavonoides/química
Medicina Tradicional Chinesa/normas
Pyrola/metabolismo
Quercetina/análogos & derivados
Quercetina/química
Quercetina/metabolismo
Especificidade da Espécie
Taninos/química
Taninos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Drugs, Chinese Herbal); 0 (Flavonoids); 0 (Tannins); 8O1CR18L82 (hyperoside); 9IKM0I5T1E (Quercetin)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140507
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0096329


  8 / 31 MEDLINE  
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[PMID]:23762895
[Au] Autor:Zhang DY; Yao XH; Duan MH; Luo M; Wang W; Fu YJ; Zu YG; Efferth T
[Ad] Endereço:State Engineering Laboratory for Bio-Resource Eco-Utilization, PR China.
[Ti] Título:An effective negative pressure cavitation-microwave assisted extraction for determination of phenolic compounds in P. calliantha H. Andr.
[So] Source:Analyst;138(16):4631-41, 2013 Aug 21.
[Is] ISSN:1364-5528
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel negative pressure and microwave assisted extraction technique (NMAE) was first proposed and applied for extraction of phenolic compounds from pyrola. [C4MIM]BF4 aqueous solution was selected as extraction solvent. Optimal extraction conditions were microwave power 700 W, negative pressure -0.07 MPa, temperature 40 °C, liquid-solid ratio 20 : 1, ionic liquid (IL) concentration 0.5 M, extraction time 15 min. The predominance of NMAE was investigated by comparing with microwave-assisted extraction (MAE) and negative pressure cavitation extraction (NPCE) using a first-order kinetics equation. The C∞ values of the target compounds by NMAE were from 0.406 to 5.977 mg g⁻¹ higher than these by MAE and NPCE, which indicated that NMAE had higher extraction yields. The K values of NMAE were also the highest; it was testified that the target compounds could be transferred from matrix into solvent much more effectively by NMAE than by MAE and NPCE. In addition, the NMAE method was validated in terms of repeatability and reproducibility, the relative standard deviation for relative recovery was lower than 5.43 and 8.78%, respectively. Therefore, NMAE was a developed extraction technique for analytical sample preparation. The RP-HPLC-UV method was also successfully applied for the quantification of six target compounds in pyrola.
[Mh] Termos MeSH primário: Extração Líquido-Líquido/métodos
Micro-Ondas
Fenóis/análise
Extratos Vegetais/análise
Pyrola/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
Pressão
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Phenols); 0 (Plant Extracts)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130614
[St] Status:MEDLINE
[do] DOI:10.1039/c3an36534d


  9 / 31 MEDLINE  
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[PMID]:23404045
[Au] Autor:Cai L; Ye H; Li X; Lin Y; Yu F; Chen J; Li H; Liu X
[Ad] Endereço:Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China.
[Ti] Título:Chemical constituents of volatile oil from Pyrolae herba and antiproliferative activity against SW1353 human chondrosarcoma cells.
[So] Source:Int J Oncol;42(4):1452-8, 2013 Apr.
[Is] ISSN:1791-2423
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:The objective of the present study was to identify chemical constituents of volatile oil from Pyrolae herba (PHVO) and evaluate the antiproliferative activity of PHVO against SW1353 human chondrosarcoma cells. The volatile oil from Pyrolae herba was prepared by hydrodistillation and characterized by gas chromatography-mass spectroscopy (GC-MS). A total of 12 components in PHVO were identified representing 81.62% of the total integrated chromatographic peaks. The major compounds were found to be n-hexadecanoic acid (29.29%), cedrol (17.08%), 6,10,14-trimethyl-2-pentadecanone (9.59%) and cis-9-octadecadienoic acid (8.23%). The antiproliferative activity of PHVO against SW1353 cells was investigated using MTT assay, flow cytometry and western blot analysis. Our results demonstrated that PHVO inhibited SW1353 cell viability in a dose- and time-dependent manner. Furthermore, PHVO treatment decreased the number of cells entering the S phase and caused a reduction in the expression of cyclin D1, cyclin-dependent kinase (CDK)4 and CDK6, whereas it caused an increase in the expression of p21. PHVO demonstrated potent antitumor activity against SW1353 cells, suggesting its potential use as a therapeutic agent in the treatment of chondrosarcoma.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Proliferação Celular/efeitos dos fármacos
Medicamentos de Ervas Chinesas/farmacologia
Óleos Voláteis/farmacologia
Óleos Vegetais/farmacologia
Pyrola/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Proteínas de Ciclo Celular/genética
Proteínas de Ciclo Celular/metabolismo
Linhagem Celular Tumoral
Forma Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Condrossarcoma
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/isolamento & purificação
Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos
Cromatografia Gasosa-Espectrometria de Massas
Expressão Gênica/efeitos dos fármacos
Seres Humanos
Óleos Voláteis/química
Óleos Voláteis/isolamento & purificação
Óleos Vegetais/química
Óleos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Cell Cycle Proteins); 0 (Drugs, Chinese Herbal); 0 (Oils, Volatile); 0 (Plant Oils)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:130301
[Lr] Data última revisão:
130301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130214
[St] Status:MEDLINE
[do] DOI:10.3892/ijo.2013.1816


  10 / 31 MEDLINE  
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[PMID]:23347020
[Au] Autor:Hynson NA; Weiß M; Preiss K; Gebauer G; Treseder KK
[Ad] Endereço:Department of Botany, University of Hawaii Manoa, Honolulu, HI 96822, USA. nhynson@hawaii.edu
[Ti] Título:Fungal host specificity is not a bottleneck for the germination of Pyroleae species (Ericaceae) in a Bavarian forest.
[So] Source:Mol Ecol;22(5):1473-81, 2013 Mar.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plants that produce dust seeds can recruit fungi to meet their earliest requirements for carbon and other nutrients. This germination strategy, termed initial mycoheterotrophy, has been well investigated among the orchid family, but there are numerous other plant lineages that have independently evolved mycoheterotrophic germination strategies. One of these lineages is the tribe Pyroleae (Ericaceae). While the fungi associated with mature plants in Pyroleae have been fairly well documented, their mycobionts at the germination and seedling stages are largely unknown. Here, we use an in situ seed baiting experiment along with molecular fingerprinting techniques and phylogenetic tests to identify the fungi associated with seedlings of two Pyroleae species, Pyrola chlorantha and Orthilia secunda. Our results indicate that similar to adult plants, Pyroleae seedlings can associate with a suite of ectomycorrhizal fungi. Some seedlings harboured single mycobionts, while others may have been inhabited by multiple fungi. The dominant seedling mycobiont of both Pyroleae species was a fungus of unknown trophic status in the order Sebacinales. This taxon was also the only one shared among seedlings of both investigated Pyroleae species. We discuss these results juxtaposed to orchids and one additional Pyrola species in the context of ontogenetic shifts in fungal host specificity for mycoheterotrophic nutrition.
[Mh] Termos MeSH primário: DNA Fúngico/isolamento & purificação
Ericaceae/microbiologia
Germinação
Micorrizas/genética
[Mh] Termos MeSH secundário: Carbono/metabolismo
DNA Fúngico/genética
Dados de Sequência Molecular
Micorrizas/classificação
Micorrizas/isolamento & purificação
Filogenia
Pyrola/microbiologia
Plântulas/microbiologia
Sementes/microbiologia
Árvores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 7440-44-0 (Carbon)
[Em] Mês de entrada:1309
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130126
[St] Status:MEDLINE
[do] DOI:10.1111/mec.12180



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