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Pesquisa : B01.650.940.800.575.912.250.341.937.774.249 [Categoria DeCS]
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[PMID]:29321009
[Au] Autor:Ben Lagha A; LeBel G; Grenier D
[Ad] Endereço:Oral Ecology Research Group (GREB), Faculty of Dentistry, Université Laval, 2420 Rue de la Terrasse, Quebec City, QC, G1V 0A6, Canada.
[Ti] Título:Dual action of highbush blueberry proanthocyanidins on Aggregatibacter actinomycetemcomitans and the host inflammatory response.
[So] Source:BMC Complement Altern Med;18(1):10, 2018 Jan 10.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The highbush blueberry (Vaccinium corymbosum) has a beneficial effect on several aspects of human health. The present study investigated the effects of highbush blueberry proanthocyanidins (PACs) on the virulence properties of Aggregatibacter actinomycetemcomitans and macrophage-associated inflammatory responses. METHODS: PACs were isolated from frozen highbush blueberries using solid-phase chromatography. A microplate dilution assay was performed to determine the effect of highbush blueberry PACs on A. actinomycetemcomitans growth as well as biofilm formation stained with crystal violet. Tight junction integrity of oral keratinocytes was assessed by measuring the transepithelial electrical resistance (TER), while macrophage viability was determined with a colorimetric MTT assay. Pro-inflammatory cytokine and MMP secretion by A. actinomycetemcomitans-stimulated macrophages was quantified by ELISA. The U937-3xκB-LUC monocyte cell line transfected with a luciferase reporter gene was used to monitor NF-κB activation. RESULTS: Highbush blueberry PACs reduced the growth of A. actinomycetemcomitans and prevented biofilm formation at sub-inhibitory concentrations. The treatment of pre-formed biofilms with the PACs resulted in a loss of bacterial viability. The antibacterial activity of the PACs appeared to involve damage to the bacterial cell membrane. The PACs protected the oral keratinocytes barrier integrity from damage caused by A. actinomycetemcomitans. The PACs also protected macrophages from the deleterious effect of leukotoxin Ltx-A and dose-dependently inhibited the secretion of pro-inflammatory cytokines (IL-1ß, IL-6, CXCL8, TNF-α), matrix metalloproteinases (MMP-3, MMP-9), and sTREM-1 by A. actinomycetemcomitans-treated macrophages. The PACs also inhibited the activation of the NF-κB signaling pathway. CONCLUSION: The antibacterial and anti-inflammatory properties of highbush blueberry PACs as well as their ability to protect the oral keratinocyte barrier and neutralize leukotoxin activity suggest that they may be promising candidates as novel therapeutic agents.
[Mh] Termos MeSH primário: Aggregatibacter actinomycetemcomitans
Mirtilos Azuis (Planta)/química
Interações Hospedeiro-Patógeno
Extratos Vegetais/farmacologia
Proantocianidinas/farmacologia
[Mh] Termos MeSH secundário: Aggregatibacter actinomycetemcomitans/efeitos dos fármacos
Aggregatibacter actinomycetemcomitans/imunologia
Citocinas/metabolismo
Interações Hospedeiro-Patógeno/efeitos dos fármacos
Interações Hospedeiro-Patógeno/imunologia
Seres Humanos
Queratinócitos/citologia
Queratinócitos/efeitos dos fármacos
Macrófagos/efeitos dos fármacos
Macrófagos/imunologia
NF-kappa B/metabolismo
Periodontite
Extratos Vegetais/química
Proantocianidinas/química
Junções Íntimas
Células U937
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (NF-kappa B); 0 (Plant Extracts); 0 (Proanthocyanidins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2072-x


  2 / 721 MEDLINE  
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[PMID]:29260547
[Au] Autor:Hong SM; Soe KH; Lee TH; Kim IS; Lee YM; Lim BO
[Ad] Endereço:BK21PLUS Glocal Education Program of Nutraceuticals Development, Konkuk University , Chungju-si, Chungcheongbuk-do 27478, Republic of Korea.
[Ti] Título:Cognitive Improving Effects by Highbush Blueberry (Vaccinium crymbosum L.) Vinegar on Scopolamine-Induced Amnesia Mice Model.
[So] Source:J Agric Food Chem;66(1):99-107, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The present study aimed to evaluate the preventive effects of highbush blueberry (Vaccinium corymbosum L.) vinegar (BV) on cognitive functions in a scopolamine (Sco)-induced amnesia model in mice. In this study, Sco (1 mg/kg, intraperitoneal injection) was used to induce amnesia. ICR mice were orally administered donepezil (5 mg/kg), blueberry extract (120 mg/kg), and BV (120 mg/kg) for 7 days. After inducing cognitive impairment by Sco, a behavioral assessment using behavior tests (i.e., Y-maze and passive avoidance tests) was performed. The BV group showed significantly restored cognitive function in the behavioral tests. BV facilitated cholinergic activity by inhibiting acetylcholinesterase activity, and enhanced antioxidant enzyme activity. Furthermore, BV was found to be rehabilitated in the cornu ammonis 1 neurons of hippocampus. In our study, we demonstrated that the memory protection conferred by BV was linked to activation of brain-derived neurotrophic factor (BDNF)/cAMP response element binding protein (CREB)/serine-threonine kinase (AKT) signaling.
[Mh] Termos MeSH primário: Amnésia/tratamento farmacológico
Mirtilos Azuis (Planta)/química
Cognição/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Acetilcolina/metabolismo
Acetilcolinesterase/metabolismo
Amnésia/induzido quimicamente
Animais
Aprendizagem da Esquiva/efeitos dos fármacos
Córtex Cerebral/efeitos dos fármacos
Córtex Cerebral/metabolismo
Modelos Animais de Doenças
Hipocampo/efeitos dos fármacos
Hipocampo/metabolismo
Aprendizagem em Labirinto
Camundongos Endogâmicos ICR
Extratos Vegetais/química
Hidrobrometo de Escopolamina/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuroprotective Agents); 0 (Plant Extracts); 451IFR0GXB (Scopolamine Hydrobromide); EC 3.1.1.7 (Acetylcholinesterase); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03965


  3 / 721 MEDLINE  
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[PMID]:27773253
[Au] Autor:Jiang HL; Yang JL; Shi YP
[Ad] Endereço:Key Laboratory of Chemistry of Northwestern Plant Resources of CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
[Ti] Título:Optimization of ultrasonic cell grinder extraction of anthocyanins from blueberry using response surface methodology.
[So] Source:Ultrason Sonochem;34:325-331, 2017 01.
[Is] ISSN:1873-2828
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ultrasonic cell grinder extraction (UCGE), using water as the solvent, was firstly applied to extract anthocyanins from blueberry. Extraction yield was related with four variables, including ratio of solution to solid, extraction power, buffer time, and extraction time. On the basis of response surface methodology (RSM), the optimal conditions were determined to be the ratio of solution to solid as 25:1(mL/g), the extraction power as 1500W, the buffer time as 3.0s, and the extraction time as 40min. The experimental yield of anthocyanins using UCGE was 2.89mg/g higher than that of conventional ultrasound-assisted extraction (CUAE). This study indicated that UCGE was an innovative, efficient, and environment friendly method in ultrasonic extraction fields, and had a potential to effectively extract other bioactive constituents.
[Mh] Termos MeSH primário: Antocianinas/isolamento & purificação
Mirtilos Azuis (Planta)/química
Fracionamento Químico/métodos
Ondas Ultrassônicas
[Mh] Termos MeSH secundário: Reprodutibilidade dos Testes
Solventes/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Solvents); 059QF0KO0R (Water)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  4 / 721 MEDLINE  
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[PMID]:28873622
[Au] Autor:Chu W; Gao H; Chen H; Fang X; Zheng Y
[Ad] Endereço:College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Food Science Institute, Zhejiang Academy of Agricultural Science, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture, Key Laboratory of Fruits and Vegetables Postharvest and Proces
[Ti] Título:Effects of cuticular wax on the postharvest quality of blueberry fruit.
[So] Source:Food Chem;239:68-74, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The blueberry fruit has a light-blue appearance because its blue-black skin is covered with a waxy bloom. This layer is easily damaged or removed during fruit harvesting and postharvest handling. We investigated the effects of wax removal on the postharvest quality of blueberry fruit and their possible mechanisms. The removal of natural wax on the fruit was found to accelerate the postharvest water loss and decay, reduce the sensory and nutritional qualities, and shorten the shelf-life. Wax removal decreased the activities of antioxidant enzymes and contents of antioxidants, and accelerated accumulation of ROS and lipid peroxidation, especially at the later period of storage. Moreover, the organellar membrane structure was disrupted in fruit with wax removed. These results indicate that cuticular wax plays an important role in maintaining the postharvest quality and delaying fruit senescence. The results should improve our understanding for better preservation of postharvest quality of blueberry fruit.
[Mh] Termos MeSH primário: Mirtilos Azuis (Planta)
[Mh] Termos MeSH secundário: Antioxidantes
Conservação de Alimentos
Frutas
Valor Nutritivo
Ceras
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Waxes)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  5 / 721 MEDLINE  
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[PMID]:28873543
[Au] Autor:Yu Y; Jin TZ; Fan X; Wu J
[Ad] Endereço:Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing/Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, No. 133, Yihenglu, Guangzhou 510610, China.
[Ti] Título:Biochemical degradation and physical migration of polyphenolic compounds in osmotic dehydrated blueberries with pulsed electric field and thermal pretreatments.
[So] Source:Food Chem;239:1219-1225, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fresh blueberries were pretreated by pulsed electric fields (PEF) or thermal pretreatment and then were subject to osmotic dehydration. The changes in contents of anthocyanins, predominantly phenolic acids and flavonols, total phenolics, polyphenol oxidase (PPO) activity and antioxidant activity in the blueberry samples during pretreatment and osmotic dehydration were investigated. Biochemical degradation and physical migration of these nutritive compounds from fruits to osmotic solutions were observed during the pretreatments and osmotic dehydration. PEF pretreated samples had the least degradation loss but the most migration loss of these compounds compared to thermally pretreated and control samples. Higher rates of water loss and solid gain during osmotic dehydration were also obtained by PEF pretreatment, reducing the dehydration time from 130 to 48h. PEF pretreated and dehydrated fruits showed superior appearance to thermally pretreated and control samples. Therefore, PEF pretreatment is a preferred technology that balances nutritive quality, appearance, and dehydration rate.
[Mh] Termos MeSH primário: Mirtilos Azuis (Planta)
[Mh] Termos MeSH secundário: Antocianinas
Fenômenos Bioquímicos
Desidratação
Frutas
Osmose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  6 / 721 MEDLINE  
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[PMID]:28961749
[Au] Autor:Van Timmeren S; Horejsi L; Larson S; Spink K; Fanning P; Isaacs R
[Ad] Endereço:Department of Entomology, Michigan State University, East Lansing, MI 48824.
[Ti] Título:Diurnal Activity of Drosophila suzukii (Diptera: Drosophilidae) in Highbush Blueberry and Behavioral Response to Irrigation and Application of Insecticides.
[So] Source:Environ Entomol;46(5):1106-1114, 2017 10 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spotted wing Drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae), is an invasive vinegar fly that has become a primary direct pest of berry crops worldwide. We conducted 2 yr of behavioral studies in blueberry plantings to determine how fly activity varied throughout the day. Observations of diurnal activity of adult D. suzukii found the greatest activity in the morning hours between 0600 and 0800 hours, when the majority of flies were on the berries. Flies were also active in the evening mainly between 1800 and 2000 hours; however, this trend was more prominent in 2015, which had cooler and more humid evenings. Experiments examining the effect of irrigation on D. suzukii behavior showed that flies remained active during and after irrigation. The effect of insecticide treatments alone and in combination with irrigation revealed that treatment with spinosad had limited effects on the number of flies per bush, whereas spinetoram reduced the number flying and on the bushes in some cases. Zeta-cypermethrin caused longer and more consistent reduction in D. suzukii flying and on bushes. In all treatments, we observed surviving flies flying near and on treated bushes, indicating that these insecticides do not completely deter fly activity. Irrigation did not influence the effects of zeta-cypermethrin on fly behavior during daily observations up to 3 d after application. Our results highlight that the diurnal patterns of activity of D. suzukii on host plants are flexible and are relatively unaffected by irrigation or insecticide applications.
[Mh] Termos MeSH primário: Irrigação Agrícola
Comportamento Animal/efeitos dos fármacos
Drosophila/efeitos dos fármacos
Inseticidas/toxicidade
[Mh] Termos MeSH secundário: Animais
Mirtilos Azuis (Planta)
Ritmo Circadiano
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insecticides)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx131


  7 / 721 MEDLINE  
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[PMID]:28764953
[Au] Autor:Tang SB; Lee JC; Jung JK; Choi MY
[Ad] Endereço:USDA ARS Horticultural Crops Research Unit and (b)Department of Horticulture, Oregon State University, 3420 NW Orchard Avenue, Corvallis, OR 97330, USA; USDA ARS Horticultural Crops Research Unit and (b)Department of Horticulture, Oregon State University, 3420 NW Orchard Avenue, Corvallis, OR 97330,
[Ti] Título:Effect of erythritol formulation on the mortality, fecundity and physiological excretion in Drosophila suzukii.
[So] Source:J Insect Physiol;101:178-184, 2017 Aug.
[Is] ISSN:1879-1611
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Previously, we studied various combinations of non-nutritive sugars including erythritol and erythrose having a potentially insecticidal effect on Drosophila suzukii. The study suggested two potential physiological changes causing fly mortality: 1) starvation from the feeding of non-metabolizable erythritol and erythrose; 2) abnormal osmotic pressure increased in the hemolymph with erythritol transported from the midgut. In the present study, sucrose and erythritol were applied to blueberries and effects of these combinations on fly mortality and fecundity were monitored in the lab and greenhouse. In the lab, two sucrose/erythritol formulations (0.5M sucrose/2M erythritol, 1M sucrose/2M erythritol) resulted in the highest mortality and the lowest fecundity among D. suzukii adults. Two formulations, therefore, were selected for further evaluation with blueberry bushes and fruits in the greenhouse; fly survival with 0.5M sucrose/2M erythritol was significantly lower than 1M sucrose/2M erythritol for 7days. Unlike the smaller container, mortality occurred faster in the greenhouse probably because flies moved more in the bigger cage accelerating the exhaustion of energy reserves in the body. We examined presence of erythritol in the hemolymph and frass to determine the nutritional metabolism and absorption of erythritol in D. suzukii. Unlike sucrose, a large amount of erythritol was observed in the hemolymph of the fly that ingested 0.5M sucrose/0.5M erythritol. Erythritol was also found in the frass of the same fly. The results imply that erythritol might be directly transported from the midgut without being metabolized and stored, but is accumulated in the hemolymph which in turn elevates the osmotic pressure in the fly hemolymph. For practical application, the sucrose/erythritol combination would be more effective than erythritol alone because the combination tastes sweeter to elicit more feeding. This erythritol formulation can be a potential insecticide used alone or as a delivery agent combined with conventional or biological insecticides to enhance their efficacy.
[Mh] Termos MeSH primário: Drosophila/efeitos dos fármacos
Drosophila/metabolismo
Eritritol/farmacologia
Inseticidas/farmacologia
Sacarose/farmacologia
[Mh] Termos MeSH secundário: Animais
Mirtilos Azuis (Planta)
Relação Dose-Resposta a Droga
Eritritol/metabolismo
Feminino
Fertilidade/efeitos dos fármacos
Hemolinfa/química
Controle de Insetos/métodos
Inseticidas/metabolismo
Masculino
Sacarose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 57-50-1 (Sucrose); RA96B954X6 (Erythritol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


  8 / 721 MEDLINE  
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[PMID]:28759392
[Au] Autor:Darby JB; Valentine G; Hillier K; Hunt R; Healy CM; Smith V; Allen-Rhoades W
[Ad] Endereço:Departments of Pediatrics and jbdarby@texaschildrens.org.
[Ti] Título:A 3-Week-Old With an Isolated "Blueberry Muffin" Rash.
[So] Source:Pediatrics;140(1), 2017 Jul.
[Is] ISSN:1098-4275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A 3-week-old boy, former 39-week term infant, presented to the emergency department with a rash. One week before presentation, he developed dark, purple papules on his forehead, which then spread to the abdomen and inguinal regions. Throughout this time, he was eating well, gaining weight, developing appropriately, and was afebrile without cough, congestion, or rhinorrhea. On presentation, the patient was well appearing with normal vital signs. His weight was 4.83 kg (86th percentile for age), his length was 56 cm (47th percentile for age), and his head circumference was 37 cm (62nd percentile for age). On skin examination, there were scattered purpuric papules and macules on the scalp, forehead, trunk, abdomen, and inguinal region. Initial laboratory studies were remarkable only for mild anemia. Our expert panel examines the case, offers a differential for a child with a "blueberry muffin" rash, and makes diagnostic considerations.
[Mh] Termos MeSH primário: Exantema/diagnóstico
Pele/patologia
[Mh] Termos MeSH secundário: Mirtilos Azuis (Planta)
Diagnóstico Diferencial
Seres Humanos
Recém-Nascido
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE


  9 / 721 MEDLINE  
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[PMID]:28699753
[Au] Autor:Xiao T; Guo Z; Sun B; Zhao Y
[Ad] Endereço:Shenyang Pharmaceutical University , Shenyang 110016, China.
[Ti] Título:Identification of Anthocyanins from Four Kinds of Berries and Their Inhibition Activity to α-Glycosidase and Protein Tyrosine Phosphatase 1B by HPLC-FT-ICR MS/MS.
[So] Source:J Agric Food Chem;65(30):6211-6221, 2017 Aug 02.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The polyphenolic profiles of four berries (blueberry, bilberry, mulberry, and cranberry) in China were investigated using Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS). Thirty-nine polyphenols including 26 anthocyanins, 9 flavonoids, and 4 phenolic acids were identified accurately. Cyanidin aglycones are common in four berries, and malvidin aglycones are the main compounds found in bilberry and cranberry. The anthocyanin level in blueberry are the highest with 739.6 ± 17.14 mg/g DW and presented the strongest antioxidant capacity in DPPH, ABTS, FRAP, and ORAC assay. In α-glycosidase, the inhibition activity was in the following order: mulberry > bilberry > blueberry > cranberry. For the PTP1B inhibition assay, blueberry demonstrated the highest inhibitory effect with IC 3.06 ± 0.02 µg/mL, followed by bilberry, mulberry, and cranberry. Molecular docking results showed that cyanidin aglycones had the highest inhibition activity to PTP1B.
[Mh] Termos MeSH primário: Antocianinas/química
Inibidores Enzimáticos/química
Frutas/química
Extratos Vegetais/química
Proteína Tirosina Fosfatase não Receptora Tipo 1/antagonistas & inibidores
[Mh] Termos MeSH secundário: Antocianinas/isolamento & purificação
Mirtilos Azuis (Planta)/química
Cromatografia Líquida de Alta Pressão
Inibidores Enzimáticos/isolamento & purificação
Inibidores de Glicosídeo Hidrolases/química
Inibidores de Glicosídeo Hidrolases/isolamento & purificação
Seres Humanos
Simulação de Acoplamento Molecular
Morus/química
Extratos Vegetais/isolamento & purificação
Proteína Tirosina Fosfatase não Receptora Tipo 1/química
Proteína Tirosina Fosfatase não Receptora Tipo 1/metabolismo
Espectrometria de Massas em Tandem
Vaccinium myrtillus/química
alfa-Glucosidases/química
alfa-Glucosidases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Enzyme Inhibitors); 0 (Glycoside Hydrolase Inhibitors); 0 (Plant Extracts); EC 3.1.3.48 (PTPN1 protein, human); EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1); EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02550


  10 / 721 MEDLINE  
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[PMID]:28629320
[Au] Autor:Song GQ; Gao X
[Ad] Endereço:Plant Biotechnology Resource and Outreach Center, Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA. songg@msu.edu.
[Ti] Título:Transcriptomic changes reveal gene networks responding to the overexpression of a blueberry DWARF AND DELAYED FLOWERING 1 gene in transgenic blueberry plants.
[So] Source:BMC Plant Biol;17(1):106, 2017 Jun 19.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Constitutive expression of the CBF/DREB1 for increasing freezing tolerance in woody plants is often associated with other phenotypic changes including dwarf plant and delayed flowering. These phenotypic changes have been observed when Arabidopsis DWARF AND DELAYED FLOWERING 1 (DDF1) was overexpressed in A. thaliana plants. To date, the DDF1 orthologues have not been studied in woody plants. The aim of this study is to investigate transcriptomic responses to the overexpression of blueberry (Vaccinium corymbosum) DDF1 (herein, VcDDF1-OX). RESULTS: The VcDDF1-OX resulted in enhanced freezing tolerance in tetraploid blueberry plants and did not result in significant changes in plant size, chilling requirement, and flowering time. Comparative transcriptome analysis of transgenic 'Legacy-VcDDF1-OX' plants containing an overexpressed VcDDF1 with non-transgenic highbush blueberry 'Legacy' plants revealed the VcDDF1-OX derived differentially expressed (DE) genes and transcripts in the pathways of cold-response, plant flowering, DELLA proteins, and plant phytohormones. The increase in freezing tolerance was associated to the expression of cold-regulated genes (CORs) and the ethylene pathway genes. The unchanged plant size, dormancy and flowering were due to the minimal effect of the VcDDF1-OX on the expression of DELLA proteins, flowering pathway genes, and the other phytohormone genes related to plant growth and development. The DE genes in auxin and cytokinin pathways suggest that the VcDDF1-OX has also altered plant tolerance to drought and high salinity. CONCLUSION: A DDF1 orthologue in blueberry functioned differently from the DDF1 reported in Arabidopsis. The overexpression of VcDDF1 or its orthologues is a new approach to increase freezing tolerance of deciduous woody plant species with no obvious effect on plant size and plant flowering time.
[Mh] Termos MeSH primário: Mirtilos Azuis (Planta)/genética
Redes Reguladoras de Genes
Genes de Plantas
[Mh] Termos MeSH secundário: Mirtilos Azuis (Planta)/crescimento & desenvolvimento
Flores/genética
Flores/crescimento & desenvolvimento
Congelamento
Plantas Geneticamente Modificadas
Termotolerância/genética
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-017-1053-z



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