Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.341.984.549 [Categoria DeCS]
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[PMID]:28138928
[Au] Autor:Shafiei M; Abdi-Ali A; Shahcheraghi F; Vali H; Shahbani Zahiri H; Akbari Noghabi K
[Ad] Endereço:Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
[Ti] Título:Analysis of Pseudomonas aeruginosa PAO1 Biofilm Protein Profile After Exposure to n-Butanolic Cyclamen coum Extract Alone and in Combination with Ciprofloxacin.
[So] Source:Appl Biochem Biotechnol;182(4):1444-1457, 2017 Aug.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudomonas aeruginosa biofilm-related infections are the major cause of premature death in cystic fibrosis patients. Strategies to induce biofilm dispersal are of interest, because of their potential in preventing biofilm-related infections. Our previous work demonstrated that n-butanolic Cyclamen coum extract with ciprofloxacin could eliminate 1- and 3-day-old P. aeruginosa PAO1 biofilms. To gain new insights into the role of C. coum extract and its synergistic effect with ciprofloxacin in eliminating P. aeruginosa PAO1 biofilms, two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry-based protein identification were used. Changes in the bacterial protein expression were analyzed when 3-day-old biofilm cells were exposed to the C. coum extract alone and in combination with ciprofloxacin. Proteins involved in alginate biosynthesis, quorum sensing, adaptation/protection, carbohydrate and amino acid metabolism showed a weaker expression in the C. coum extract-ciprofloxacin-treated biofilm cells compared to those in the untreated cells. Interestingly, the proteome of C. coum extract-ciprofloxacin-treated biofilm revealed more resemblance to the planktonic phenotype than to the biofilm phenotype. It appears that saponin extract in combination with ciprofloxacin causes biofilm disruption due to several mechanisms such as motility induction, cell envelope integrity perturbation, stress protein expression reduction, and more importantly, signal transduction perturbation. In conclusion, exposure to a combination of biofilm dispersal such as saponin extract and antimicrobial agents may offer a novel strategy to control preestablished, persistent P. aeruginosa biofilms and biofilm-related infections.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Biofilmes
Ciprofloxacino/farmacologia
Cyclamen/química
Extratos Vegetais/farmacologia
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/metabolismo
[Mh] Termos MeSH secundário: Adaptação Fisiológica/efeitos dos fármacos
Aminoácidos/metabolismo
Biofilmes/efeitos dos fármacos
Biomassa
Butanóis/química
Carbono/metabolismo
Cyclamen/metabolismo
Sinergismo Farmacológico
Ácidos Graxos/metabolismo
Lipopolissacarídeos/metabolismo
Fosfolipídeos/metabolismo
Pseudomonas aeruginosa/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Bacterial Proteins); 0 (Butanols); 0 (Fatty Acids); 0 (Lipopolysaccharides); 0 (Phospholipids); 0 (Plant Extracts); 5E8K9I0O4U (Ciprofloxacin); 7440-44-0 (Carbon)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-017-2409-4


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[PMID]:27604272
[Au] Autor:Simsek O; Curuk P; Aslan F; Bayramoglu M; Izgu T; da Silva JA; Kacar YA; Mendi YY
[Ad] Endereço:Department of Horticulture, Faculty of Agriculture, University of Çukurova, 01330, Adana, Turkey.
[Ti] Título:Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers.
[So] Source:Biochem Genet;55(1):87-102, 2017 Feb.
[Is] ISSN:1573-4927
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genus Cyclamen (family Myrsinaceae) contains about 20 species, most of which occur in the Mediterranean region. Turkey has critically important Cyclamen genetic resources. Molecular characterization of plant materials collected from different regions of Turkey in which Cyclamen species grow naturally, namely Adana, Antalya, Aydin, Mugla, Izmir, Denizli, Kahramanmaras, Osmaniye, Eskisehir, Trabzon, and Rize provinces, was performed using RAPD and SRAP markers. DNA was successfully amplified by 30 RAPD primers and 14 SRAP primer pairs. Among the 470 bands generated by the RAPD primers, 467 were polymorphic. The number of bands detected by a single primer set ranged from 11 to 22 (average of 15.6). The percentage polymorphism was 99.3 % based on the RAPD data. In the SRAP analysis, a total of 216 bands were generated, showing 100 % polymorphism. The number of bands detected by a single primer set ranged from 9 to 22 (average of 15.4). All data were scored and UPGMA dendrograms were constructed with similar results in both marker systems, i.e., different species from nine provinces of Turkey were separated from each other in the dendrograms with the same species being clustered together.
[Mh] Termos MeSH primário: Cyclamen/genética
Marcadores Genéticos
[Mh] Termos MeSH secundário: Cyclamen/classificação
Genótipo
Filogenia
Polimorfismo Genético
Técnica de Amplificação ao Acaso de DNA Polimórfico
Turquia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160909
[St] Status:MEDLINE
[do] DOI:10.1007/s10528-016-9770-9


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[PMID]:26926302
[Au] Autor:Jurkiewicz D; Hassmann-Poznanska E; Kazmierczak H; Skladzien J; Pietruszewska W; Burduk P; Rapiejko P
[Ad] Endereço:Department of Otolaryngology with Division of Cranio-Maxillo-Facial Surgery in Military Institute of Medicine, Warsaw, Poland.
[Ti] Título:Lyophilized Cyclamen europaeum tuber extract in the treatment of rhinosinusitis.
[So] Source:Otolaryngol Pol;70(1):1-9, 2016 Feb 29.
[Is] ISSN:2300-8423
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Nasal and sinus mucositis is a significant health problem associated with significant organizational and financial burden for the health care system. In recent years, several important guidelines and positions of expert groups and scientific associations have been published with regard to the diagnostics and treatment of rhinosinusitis, including European Position Paper on Rhinosinusitis and Nasal Polyps (EPOS 2012) [1] and Polish Standards for the Treatment of Rhinitis (PoSLeNN 2013) [2]. The management of viral and postviral rhinosinusitis involves systemic treatment including administration of plant origin products. The goal of this article is to present the current knowledge on the use of intranasal preparations containing natural saponin fractions from the rhizomes of Alpine cyclamen (Cyclamen europaeum). Saponins contained in the extract of Alpine cyclamen (Cyclamen europaeum) rhizomes are surface-active compounds that reduce the surface tension on the nasal mucosal cells while simultaneously stimulating the trigeminal nerve receptors leading to increased production of seromucous secretion and extensive drainage of the nasal and sinus cavities. The analysis of published studies on the efficacy and safety of intranasal products containing lyophilized extracts from Cyclamen europaeum tuber warrants the conclusion that these products are useful in the management of nasal and sinus mucositis due to their beneficial impact on the course of the treatment of acute rhinosinusitis. When used in patients with acute rhinosinusitis, an intranasal preparation containing lyophilized extracts from Cyclamen europaeum tuber efficiently reduces the symptoms, particularly the feeling of pressure and pain in the face. According to the authors of PROSINUS study, single-agent treatment using Cyclamen europaeum extracts is more efficient (in terms of the percentage of success) than other monotherapy or combination regimens.
[Mh] Termos MeSH primário: Cyclamen
Fitoterapia/métodos
Preparações de Plantas/uso terapêutico
Rinite/tratamento farmacológico
Sinusite/tratamento farmacológico
[Mh] Termos MeSH secundário: Doença Aguda
Administração Intranasal
Relação Dose-Resposta a Droga
Seres Humanos
Sprays Nasais
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nasal Sprays); 0 (Plant Preparations)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.5604/00306657.1195609


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[PMID]:25720737
[Au] Autor:Rydlová J; Sýkorová Z; Slavíková R; Turis P
[Ad] Endereço:Institute of Botany, Academy of Sciences of the Czech Republic, Zámek 1, 252 43, Pruhonice, Czech Republic. jana.rydlova@ibot.cas.cz.
[Ti] Título:The importance of arbuscular mycorrhiza for Cyclamen purpurascens subsp. immaculatum endemic in Slovakia.
[So] Source:Mycorrhiza;25(8):599-609, 2015 Nov.
[Is] ISSN:1432-1890
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:At present, there is no relevant information on arbuscular mycorrhiza and the effect of the symbiosis on the growth of wild populations of cyclamens. To fill this gap, two populations of Cyclamen purpurascens subsp. immaculatum, endemic in Nízke Tatry (NT) mountains and Velká Fatra (VF) mountains, Slovakia, were studied in situ as well as in a greenhouse pot experiment. For both populations, mycorrhizal root colonization of native plants was assessed, and mycorrhizal inoculation potential (MIP) of the soils at the two sites was determined in 3 consecutive years. In the greenhouse experiment, the growth response of cyclamens to cross-inoculation with arbuscular mycorrhizal fungi (AMF) was tested: plants from both sites were grown in their native soils and inoculated with a Septoglomus constrictum isolate originating either from the same or from the other plant locality. Although the MIP of soil at the NT site was significantly higher than at the VF site, the level of AMF root colonization of C. purpurascens subsp. immaculatum plants in the field did not significantly differ between the two localities. In the greenhouse experiment, inoculation with AMF generally accelerated cyclamen growth and significantly increased all growth parameters (shoot dry weight, leaf number and area, number of flowers, tuber, and root dry weight) and P uptake. The two populations of C. purpurascens subsp. immaculatum grown in their native soils, however, differed in their response to inoculation. The mycorrhizal growth response of NT plants was one-order higher compared to VF plants, and all their measured growth parameters were stimulated regardless of the fungal isolates' origin. In the VF plants, only the non-native (NT originating) isolate showed a significant positive effect on several growth traits. It can be concluded that mycorrhiza significantly increased fitness of C. purpurascens subsp. immaculatum, despite the differences between plant populations, implying that AMF symbionts should be taken into account in conservation programs of this endemic plant.
[Mh] Termos MeSH primário: Cyclamen/microbiologia
Micorrizas/fisiologia
[Mh] Termos MeSH secundário: Cyclamen/crescimento & desenvolvimento
Micorrizas/genética
Micorrizas/crescimento & desenvolvimento
Micorrizas/isolamento & purificação
Filogenia
Raízes de Plantas/microbiologia
Eslováquia
Microbiologia do Solo
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1607
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150228
[St] Status:MEDLINE
[do] DOI:10.1007/s00572-015-0634-7


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[PMID]:24649909
[Au] Autor:El Hosry L; Di Giorgio C; Birer C; Habib J; Tueni M; Bun SS; Herbette G; De Meo M; Ollivier E; Elias R
[Ad] Endereço:Laboratoire de Pharmacognosie et Ethnopharmacologie, UMR MD3, Faculté de Pharmacie, Aix-Marseille Université , Marseille Cedex , France .
[Ti] Título:In vitro cytotoxic and anticlastogenic activities of saxifragifolin B and cyclamin isolated from Cyclamen persicum and Cyclamen libanoticum.
[So] Source:Pharm Biol;52(9):1134-40, 2014 Sep.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: The genus Cyclamen L. (Primulaceae) is rich in saponins known to have interesting biological activities. OBJECTIVE: To isolate saxifragifolin B and cyclamin, two triterpene saponins, from Cyclamen libanoticum Hildebr and Cyclamen persicum Mill, and to assess their cytotoxic, clastogenic/aneugenic, and anticlastogenic effects, as well as antioxidant potential. MATERIALS AND METHODS: Saxifragifolin B and cyclamin were tested for their cytotoxicity against SK-BR-3, HT-29, HepG2/3A, NCI-H1299, BXPC-3, 22RV1, and normal DMEM cell lines using WST-1 assay. Their clastogenic/aneugenic activities and anticlastogenic effects against the anticancer drug mitomycin C were assessed by the in vitro micronucleus assay in CHO cells. Their antioxidant capacities were determined using Fe(2+)-chelating and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. RESULTS: Both saponins were described for the first time in Cyclamen libanoticum. They showed strong cytotoxic activities against the tested cancer cell lines. Saxifragifolin B was found to be 56- and 37-times more active than mitomycin C against breast adenocarcinoma (SK-BR-3) and lung carcinoma (NCI-H1299), respectively. Also, saxifragifolin B did not induce micronuclei formation and prevented cells from mitomycin C clastogenic effect. Cyclamin induced a significant increase of micronucleated cells after metabolic activation with S9 mix, and did not possess any anticlastogenic activity. Both molecules exhibited low antioxidant activities as compared to reference compounds. DISCUSSION AND CONCLUSIONS: This study showed the remarkable cytotoxic activity of saxifragifolin B, especially against breast adenocarcinoma and lung carcinoma and its chemoprotective activity against mitomycin C. Thus, saxifragifolin B could be suggested as a potential cytotoxic drug with a preventive effect against possible exposures to genotoxic agents.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Cyclamen/química
Saponinas/farmacologia
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antibióticos Antineoplásicos/farmacologia
Antibióticos Antineoplásicos/toxicidade
Antimutagênicos/isolamento & purificação
Antimutagênicos/farmacologia
Antimutagênicos/toxicidade
Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/toxicidade
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Células CHO
Linhagem Celular
Linhagem Celular Tumoral
Cricetulus
Seres Humanos
Testes para Micronúcleos
Mitomicina/farmacologia
Mitomicina/toxicidade
Neoplasias/tratamento farmacológico
Neoplasias/patologia
Saponinas/isolamento & purificação
Saponinas/toxicidade
Triterpenos/isolamento & purificação
Triterpenos/toxicidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibiotics, Antineoplastic); 0 (Antimutagenic Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Saponins); 0 (Triterpenes); 0 (cyclamin); 0 (saxifragifolin B); 50SG953SK6 (Mitomycin)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:140813
[Lr] Data última revisão:
140813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140322
[St] Status:MEDLINE
[do] DOI:10.3109/13880209.2013.879600


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[PMID]:24289599
[Au] Autor:Yildiz M; Bozcu H; Tokgun O; Karagur ER; Akyurt O; Akca H
[Ad] Endereço:Department of Medical Oncology, Antalya Education and Research Hospital, Antalya, Turkey E-mail : drmyildiz@yahoo.com.
[Ti] Título:Cyclamen exerts cytotoxicity in solid tumor cell lines: a step toward new anticancer agents?
[So] Source:Asian Pac J Cancer Prev;14(10):5911-3, 2013.
[Is] ISSN:2476-762X
[Cp] País de publicação:Thailand
[La] Idioma:eng
[Ab] Resumo:Cyclamen coum is a traditional medicinal plant in the Turkey. Its anticancer properties and whether cyclamen extract induces any cytotoxicity in solid cancer cell lines have not been thoroughly investigated previously. Therefore we examined cytotoxic effects on cervical cancer, HeLa, and non small cell lung cancer cell, H1299, lines. Cyclamen extract induced cellular death of both HeLa and H1299 cells in a dose dependent manner. We also analyzed the capacity of cyclamen extract to induce apoptosis by the TUNEL method. Here, we for the first time report that the extract of Cyclamen coum, an endemic plant for Turkey, can induce cytotoxicity via apoptosis in HeLa and H1299 cells. These results imply that cyclamen extract can be further analyzed to potentially find novel anticancer compounds.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Carcinoma Pulmonar de Células não Pequenas/patologia
Proliferação Celular/efeitos dos fármacos
Cyclamen/química
Fitoterapia
Extratos Vegetais/farmacologia
Neoplasias do Colo do Útero/patologia
[Mh] Termos MeSH secundário: Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico
Feminino
Citometria de Fluxo
Seres Humanos
Marcação In Situ das Extremidades Cortadas
Neoplasias Pulmonares/tratamento farmacológico
Neoplasias Pulmonares/patologia
Células Tumorais Cultivadas
Turquia
Neoplasias do Colo do Útero/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131203
[St] Status:MEDLINE


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[PMID]:24026510
[Au] Autor:Tanaka Y; Oshima Y; Yamamura T; Sugiyama M; Mitsuda N; Ohtsubo N; Ohme-Takagi M; Terakawa T
[Ad] Endereço:Central Research Laboratories, Hokko Chemical Industry Co., Ltd, 2165 Toda, Atsugi, Kanagawa 243-0023, Japan.
[Ti] Título:Multi-petal cyclamen flowers produced by AGAMOUS chimeric repressor expression.
[So] Source:Sci Rep;3:2641, 2013.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cyclamen persicum (cyclamen) is a commercially valuable, winter-blooming perennial plant. We cloned two cyclamen orthologues of AGAMOUS (AG), CpAG1 and CpAG2, which are mainly expressed in the stamen and carpel, respectively. Cyclamen flowers have 5 petals, but expression of a chimeric repressor of CpAG1 (CpAG1-SRDX) caused stamens to convert into petals, resulting in a flower with 10 petals. By contrast, CpAG2-SRDX only caused incomplete formation of stamens and carpels. Expression in Arabidopsis thaliana showed similar effects on flower organ specification. Simultaneous expression of CpAG1-SRDX and CpAG2-SRDX in cyclamen induced rose-like, multi-petal flowers, a potentially valuable trait in commercial ornamental varieties. Expression of CpAG2-SRDX in a cyclamen mutant lacking expression of CpAG1 more effectively produced multi-petal flowers. Here, we controlled the number of petals in cyclamen by simple genetic engineering with a chimeric repressor. This strategy may be applicable useful for other ornamental plants with two distinct AG orthologues.
[Mh] Termos MeSH primário: Cyclamen/genética
Flores/genética
Proteínas de Domínio MADS/genética
Proteínas de Plantas/genética
Proteínas Recombinantes de Fusão/genética
Proteínas Repressoras/genética
[Mh] Termos MeSH secundário: Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Fenótipo
Plantas Geneticamente Modificadas
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MADS Domain Proteins); 0 (Plant Proteins); 0 (Recombinant Fusion Proteins); 0 (Repressor Proteins)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:150422
[Lr] Data última revisão:
150422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130913
[St] Status:MEDLINE
[do] DOI:10.1038/srep02641


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[PMID]:23887377
[Au] Autor:Svistushkin VM; Nikiforova GN; Vlasova NP
[Ti] Título:[Experience with the application of sinuforte as starting monotherapy of acute rhinosinusitis associated with exudative otitis media in children and adolescents].
[So] Source:Vestn Otorinolaringol;(3):56-60, 2013.
[Is] ISSN:0042-4668
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:A number of clinical cases of inflammatory changes in the nasopharynx rhinosinusitis apply to the area of auditory tube with the development of exudative otitis media. The objective of the present simple open study was to estimate the effectiveness and safety of the treatment of moderately severe acute rhinosinusitis associated with exudative otitis media in the children and adolescents with the use of sinuforte as starting monotherapy. The study included 47 patients at the age from 7 to 15 years. The results obtained indicate that the effectiveness of this treatment is comparable in terms of duration with that of traditional therapy. However, the use of sinuforte as initial monotherapy has some advantages over the traditional modality in that it reduces the drug loading and offers a simple scheme of the treatment with proven safety. Taken together, these advantages provide a conclusive argument in favour of selecting Sinuforte as initial therapy of the above ENT pathology.
[Mh] Termos MeSH primário: Cyclamen
Otite Média com Derrame/tratamento farmacológico
Fitoterapia/métodos
Preparações de Plantas/uso terapêutico
Rinite/tratamento farmacológico
Sinusite/tratamento farmacológico
[Mh] Termos MeSH secundário: Doença Aguda
Adolescente
Criança
Feminino
Seres Humanos
Masculino
Resultado do Tratamento
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Preparations)
[Em] Mês de entrada:1309
[Cu] Atualização por classe:130726
[Lr] Data última revisão:
130726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130727
[St] Status:MEDLINE


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[PMID]:23759862
[Au] Autor:Yokoyama S; Adachi Y; Asakura S; Kohyama E
[Ad] Endereço:Department of Environmental and Chemical Research, Industrial Technology Center, Gifu Prefectural Government, Hashima, Gifu 501-6064, Japan. yokoyama-shinichiro@pref.gifu.lg.jp
[Ti] Título:Characterization of Alcaligenes faecalis strain AD15 indicating biocontrol activity against plant pathogens.
[So] Source:J Gen Appl Microbiol;59(2):89-95, 2013.
[Is] ISSN:1349-8037
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Bacterial strain possessing both bacteriostatic and fungistatic activity (biocontrol activity) against pathogens of cyclamen (Cyclamen sp.) was isolated from the soil in Gifu Prefecture, Japan, and characterized with respect to its taxonomic and biocontrol properties. The sequence of its 16S rRNA gene, morphology, biochemistry, and fatty acid composition demonstrated that it is a strain most closely related to Alcaligenes faecalis subsp. faecalis LMG 1229(T). The isolate was named A. faecalis strain AD15. A. faecalis AD15 produced hydroxylamine at maximum yields of 33.3±1.7 mg/L after 16 h cultivation in LB medium and 19.0±0.44 mg/L after 19 h cultivation in synthetic medium. Moreover, minimum inhibitory concentrations of hydroxylamine against the cyclamen pathogens Pantoea agglomerans and Colletotrichum gloeosporioides were 4.20±0.98 and 16.5±0.67 mg/L. These results indicated that the biocontrol activity of strain AD15 might be attributed to hydroxylamine, a metabolite in the culture medium, and it had the potential for biopesticide application.
[Mh] Termos MeSH primário: Alcaligenes faecalis/classificação
Alcaligenes faecalis/fisiologia
Anti-Infecciosos/metabolismo
Antibiose
Cyclamen/microbiologia
Hidroxilamina/metabolismo
Microbiologia do Solo
[Mh] Termos MeSH secundário: Alcaligenes faecalis/genética
Alcaligenes faecalis/isolamento & purificação
Técnicas de Tipagem Bacteriana
Análise por Conglomerados
Colletotrichum/efeitos dos fármacos
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Ácidos Graxos/análise
Japão
Microscopia de Força Atômica
Dados de Sequência Molecular
Pantoea/efeitos dos fármacos
Controle Biológico de Vetores/métodos
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 2FP81O2L9Z (Hydroxylamine)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:130613
[Lr] Data última revisão:
130613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130614
[St] Status:MEDLINE


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[PMID]:23711704
[Au] Autor:Oz E; Koc S; Dusen OD; Mammadov R; Cetin H
[Ad] Endereço:Akdeniz University, Faculty of Science, Department of Biology, 07058 Antalya, Turkey.
[Ti] Título:Larvicidal activity of Cyclamen (Myrsinaceae) extracts against the larvae of West Nile virus vector Culex pipiens L. (Diptera: Culicidae).
[So] Source:Asian Pac J Trop Med;6(6):449-52, 2013 Jun.
[Is] ISSN:2352-4146
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen Cyclamen mirabile Hildebr. and Cyclamen alpinum Dammann ex. Sprenger) against Culex pipiens L. (Diptera: Culicidae). METHODS: Twenty young (first-second) or older (third-fourth) instars of the Culex pipiens were exposed to various concentrations (100 to 1000 ppm) of the extracts. Mortality was recorded after 12-, 24-, 48-, 72- and 96-h of exposure. RESULTS: We determined that the young larval stages were more susceptible to the extracts in comparison with the older larval stages. A comparison of LC 50 and LC 90 values has shown that Cyclamen mirabile was more active than Cyclamen alpinum. CONCLUSIONS: This study is the first to report on the larvicidal activity of the extracts of Cyclamen species against Culex pipiens. More studies are needed to isolate and identify the active components involved, their mode of action, and effects on other mosquito and pest species.
[Mh] Termos MeSH primário: Culex/efeitos dos fármacos
Cyclamen/química
Inseticidas/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Larva/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insecticides); 0 (Plant Extracts)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:160519
[Lr] Data última revisão:
160519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130529
[St] Status:MEDLINE



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