Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.341.998.500 [Categoria DeCS]
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  1 / 291 MEDLINE  
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[PMID]:29037694
[Au] Autor:Shi T; Zhu M; Chen Y; Yan X; Chen Q; Wu X; Lin J; Xie M
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China.
[Ti] Título:H NMR combined with chemometrics for the rapid detection of adulteration in camellia oils.
[So] Source:Food Chem;242:308-315, 2018 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Proton nuclear magnetic resonance ( H NMR) and chemometrics were employed to detect the adulteration of camellia oil (CAO) with 3 different cheap vegetable oils. With the intensity of 15 selected H NMR signals as input variables, principal component analysis (PCA) showed good group clustering results for pure and nonpure CAO, but unsatisfied identification accuracy for the adulterated oil types, indicating relatively small difference among those oils. Whereas these difference could be revealed by orthogonal projection to latent structures discriminant analysis (OPLS-DA), with identification accuracy higher than 90%. Partial least squares (PLS) was further applied for the prediction of adulteration level in CAO. With less than 6 variables screened out by variable importance in the projection (VIP) scores as potential key markers, the developed PLS models showed better accuracy. The prediction results for 10 hold-out samples also confirmed that this method was accurate and fast for the detection of CAO adulteration.
[Mh] Termos MeSH primário: Camellia/química
Contaminação de Alimentos/análise
Óleos Vegetais/análise
Espectroscopia de Prótons por Ressonância Magnética/métodos
[Mh] Termos MeSH secundário: Análise Discriminante
Análise de Componente Principal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Oils)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE


  2 / 291 MEDLINE  
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[PMID]:28759610
[Au] Autor:Dong B; Wu B; Hong W; Li X; Li Z; Xue L; Huang Y
[Ad] Endereço:South China Agricultural University, Guangzhou, Guangdong, China.
[Ti] Título:Transcriptome analysis of the tea oil camellia (Camellia oleifera) reveals candidate drought stress genes.
[So] Source:PLoS One;12(7):e0181835, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The tea-oil camellia (Camellia oleifera) is the most important oil plant in southern China, and has a strong resistance to drought and barren soil. Understanding the molecular mechanisms of drought tolerance would greatly promote its cultivation and molecular breeding. RESULTS: In total, we obtained 76,585 unigenes with an average length of 810 bp and an N50 of 1,092 bp. We mapped all the unigenes to the NCBI 'nr' (non-redundant), SwissProt, KEGG, and clusters of orthologous groups (COG) databases, where 52,531 (68.6%) unigenes were functionally annotated. According to the annotation, 46,171 (60.8%) unigenes belong to 338 KEGG pathways. We identified a series of unigenes that are related to the synthesis and regulation of abscisic acid (ABA), the activity of protective enzymes, vitamin B6 metabolism, the metabolism of osmolytes, and pathways related to the biosynthesis of secondary metabolites. After exposed to drought for 12 hours, the number of differentially-expressed genes (DEGs) between treated plants and control plants increased in the G4 cultivar, while there was no significant increase in the drought-tolerant C3 cultivar. DEGs associated with drought stress responsive pathways were identified by KEGG pathway enrichment analysis. Moreover, we found 789 DEGs related to transcription factors. Finally, according to the results of qRT-PCR, the expression levels of the 20 unigenes tested were consistent with the results of next-generation sequencing. CONCLUSIONS: In the present study, we identified a large set of cDNA unigenes from C. oleifera annotated using public databases. Further studies of DEGs involved in metabolic pathways related to drought stress and transcription will facilitate the discovery of novel genes involved in resistance to drought stress in this commercially important plant.
[Mh] Termos MeSH primário: Camellia/genética
Secas
Estresse Fisiológico/genética
Transcriptoma
[Mh] Termos MeSH secundário: Ácido Abscísico/química
China
DNA Complementar/metabolismo
Bases de Dados de Proteínas
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Biblioteca Gênica
Genoma de Planta
Sequenciamento de Nucleotídeos em Larga Escala
Lipoxigenase/metabolismo
Malondialdeído/química
Redes e Vias Metabólicas
Análise de Componente Principal
RNA de Plantas/genética
Espécies Reativas de Oxigênio/metabolismo
Vitamina B 6/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (RNA, Plant); 0 (Reactive Oxygen Species); 4Y8F71G49Q (Malondialdehyde); 72S9A8J5GW (Abscisic Acid); 8059-24-3 (Vitamin B 6); EC 1.13.11.12 (Lipoxygenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181835


  3 / 291 MEDLINE  
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[PMID]:28562049
[Au] Autor:Tu PS; Tung YT; Lee WT; Yen GC
[Ad] Endereço:Department of Food Science and Biotechnology, National Chung Hsing University , 145 Xingda Road, Taichung 40227, Taiwan.
[Ti] Título:Protective Effect of Camellia Oil (Camellia oleifera Abel.) against Ethanol-Induced Acute Oxidative Injury of the Gastric Mucosa in Mice.
[So] Source:J Agric Food Chem;65(24):4932-4941, 2017 Jun 21.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Camellia oil, a common edible oil in Taiwan and China, has health effects for the gastrointestinal tract in folk medicine, and it contains abundant unsaturated fatty acids and phytochemicals. However, the preventive effect of camellia oil on ethanol-induced gastric ulcers remains unclear. This study was aimed to evaluate the preventive effect of camellia oil on ethanol-induced gastric injury in vitro and in vivo as well as its mechanisms of action. In an in vitro study, our results showed that pretreatment of RGM-1 cells with camellia oil enhanced the migration ability as well as increased heat shock protein expression and reduced apoptotic protein expression. In animal experiments, mice pretreated with camellia oil effectively showed improved ethanol-induced acute injury of the gastric muscosa and oxidative damage through the enhancement of antioxidant enzyme activities and heat shock protein and PGE production, as well as the suppression of lipid peroxidation, apoptosis-related proteins, pro-inflammatory cytokines, and NO production. Histological injury score and hemorrhage score in ethanol-induced gastric mucosal damage dramatically elevated from the control group (0.00 ± 0.0) to 3.40 ± 0.7 and 2.60 ± 0.5, respectively. However, treatments with camellia oil or olive oil (2 mL/kg bw) and lansoprazole (30 mg/kg bw) showed significant decreases in elevation of injury score and hemorrhage score (p < 0.05). Therefore, camellia oil has the potential to ameliorate ethanol-induced acute gastric mucosal injury through the inhibition of inflammation and oxidative stress.
[Mh] Termos MeSH primário: Camellia/química
Etanol/toxicidade
Extratos Vegetais/administração & dosagem
Óleos Vegetais/administração & dosagem
Úlcera Gástrica/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Mucosa Gástrica/efeitos dos fármacos
Mucosa Gástrica/lesões
Seres Humanos
Peroxidação de Lipídeos/efeitos dos fármacos
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Estresse Oxidativo/efeitos dos fármacos
Úlcera Gástrica/induzido quimicamente
Úlcera Gástrica/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Plant Oils); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01135


  4 / 291 MEDLINE  
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[PMID]:28546125
[Au] Autor:Lei H; Su S; Ma L; Wen Y; Wang X
[Ad] Endereço:Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, 35 East Qinghua Road, Beijing 100083, China.
[Ti] Título:Molecular cloning and functional characterization of CoFT1, a homolog of FLOWERING LOCUS T (FT) from Camellia oleifera.
[So] Source:Gene;626:215-226, 2017 Aug 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:FLOWERING LOCUS T (FT) is an important integrator of flowering genetic pathways and plays crucial roles in flowering transition. The tea-oil tree (Camellia oleifera Abel.) is a valuable woody oil crop, but the molecular mechanisms controlling flowering are still unclear. In this study, a FT-like gene, CoFT1, was isolated and characterized from C. oleifera. The CoFT1 protein was localized in the nucleus and cytoplasm of Arabidopsis protoplasts, and exhibited no transactivation activity in yeast cells. CoFT1 was highly expressed in mature leaves and reproductive organs, such as flower buds, flowers, flower organs, and young fruits. Its expression showed diurnal rhythms under both long-day and short-day conditions, and was photoperiod-dependent. Seasonal expression analysis revealed that the CoFT1 transcript in leaves increased during the floral induction period. Overexpression of CoFT1 in wild-type Arabidopsis resulted in precocious flowering and elevated the transcription levels of flowering related genes, such as SOC1, AP1, and LFY. Furthermore, the yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that CoFT1 was able to interact with both Arabidopsis FD and C. oleifera CoFD proteins. The sequence analysis revealed that the CoFT1 promoter contained a number of light-responsive elements, several hormonal- and stress-responsive motifs, and flowering related transcriptional factor binding sites, including CORE, CCAATBOX1, and CArG motifs. Our results suggested that CoFT1 might function as a flowering promoter in C. oleifera.
[Mh] Termos MeSH primário: Camellia/genética
Flores/genética
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Proteínas de Plantas/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Arabidopsis/genética
Camellia/crescimento & desenvolvimento
Clonagem Molecular
Flores/crescimento & desenvolvimento
Teste de Complementação Genética
Proteínas de Plantas/metabolismo
Ligação Proteica
Elementos de Resposta
Fatores de Transcrição/metabolismo
Técnicas do Sistema de Duplo-Híbrido
Leveduras/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE


  5 / 291 MEDLINE  
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[PMID]:28537781
[Au] Autor:Lim HJ; Kim MS; Kim DS; Kim YJ; Lee JH; Pan JH; Shin EC; Kim JK
[Ad] Endereço:1 Department of Food Science, Gyeongnam National University of Science and Technology , Jinju, Korea.
[Ti] Título:Blood Pressure-Lowering Effects of Alacalase-Hydrolyzed Camellia Seed Hull In Vitro and in Spontaneous Hypertensive Rats.
[So] Source:J Med Food;20(7):720-723, 2017 Jul.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:High blood pressure is one of the major risk factors for various diseases and angiotensin-converting enzyme (ACE) plays a critical role in blood pressure regulation. In our study, the responsive surface methodology was adopted to establish optimal Alcalase-hydrolysis conditions of camellia seed hull against ACE activity. The optimum conditions are hydrolysis temperature of 50.98°C, enzyme/substrate ratio of 2.85%, and hydrolysis pH of 7.12. In an animal feeding study, spontaneously hypertensive rats were treated with either a low or high dose of hydrolyzed Camellia japonica seed cake over 5 weeks. Even though systolic blood pressure was not statistically different, the high dose of C. japonica hydrolysate lowered diastolic blood pressure (106 ± 4.4 mmHg vs. 145 ± 5.9 mmHg) at the 5th week. A similar trend was also observed in serum ACE activity. Considering that this camellia seed hull is a major resource of this plant, our study provides important data to utilize this plant for both academic and industrial applications.
[Mh] Termos MeSH primário: Inibidores da Enzima Conversora de Angiotensina/metabolismo
Anti-Hipertensivos/metabolismo
Camellia/metabolismo
Hipertensão/dietoterapia
Extratos Vegetais/metabolismo
Sementes/metabolismo
[Mh] Termos MeSH secundário: Animais
Pressão Sanguínea
Camellia/química
Seres Humanos
Hidrólise
Hipertensão/metabolismo
Hipertensão/fisiopatologia
Masculino
Peptídeo Hidrolases/química
Peptidil Dipeptidase A/metabolismo
Ratos
Ratos Endogâmicos SHR
Sementes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiotensin-Converting Enzyme Inhibitors); 0 (Antihypertensive Agents); 0 (Plant Extracts); EC 3.4.- (Peptide Hydrolases); EC 3.4.15.1 (Peptidyl-Dipeptidase A)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.1089/jmf.2016.0175


  6 / 291 MEDLINE  
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[PMID]:28433872
[Au] Autor:Karthik R; Govindasamy M; Chen SM; Cheng YH; Muthukrishnan P; Padmavathy S; Elangovan A
[Ad] Endereço:Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao, East Road, Taipei 106, Taiwan, ROC.
[Ti] Título:Biosynthesis of silver nanoparticles by using Camellia japonica leaf extract for the electrocatalytic reduction of nitrobenzene and photocatalytic degradation of Eosin-Y.
[So] Source:J Photochem Photobiol B;170:164-172, 2017 May.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In the present study, sphere-like silver nanoparticles (Ag-NPs) were synthesized by using Camellia japonica leaf extract and its remediation industrial pollutants such as nitrobenzene and Eosin-Y (EY). As-prepared sphere-like Ag-NPs were characterized by various analytical and spectroscopic methods such as UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), High-resolution transmission electron microscopy (HR-TEM), Energy dispersive X-ray spectra (EDX), and the chemical constituents of the leaf extract were also analyzed by using Gas chromatography and Mass Spectroscopy (GC-MS). Fascinatingly, the as-prepared sphere-like Ag-NPs exhibits excellent electrocatalytic and photocatalytic activity for the reduction of nitrobenzene and photo-degradation of EY dye respectively. The Cyclic voltammetry (CV) and amperometric (i-t) studies realized that the electrochemical behavior of sphere-like Ag-NPs modified electrode on nitrobenzene reduction. The proposed nitrobenzene sensor exhibited appreciable wide linear response range and low detection limit of 0.05-21µM, 23-2593µM and 12nM, respectively. The Ag-NPs modified electrode showed excellent selectivity towards the nitrobenzene detection even in the presence of common metal ions and nitroaromatic containing substances. On the other hand, Ag-NPs have excellent photocatalytic activity with >97% degradation of EY dye after irradiated 60min. These results indicated that the growth of sphere-like Ag-NPs should be a proficient.
[Mh] Termos MeSH primário: Camellia/química
Amarelo de Eosina-(YS)/química
Nanopartículas Metálicas/química
Nitrobenzenos/química
Extratos Vegetais/química
Prata/química
[Mh] Termos MeSH secundário: Camellia/metabolismo
Catálise
Técnicas Eletroquímicas
Cromatografia Gasosa-Espectrometria de Massas
Concentração de Íons de Hidrogênio
Luz
Nanopartículas Metálicas/ultraestrutura
Fotólise/efeitos da radiação
Extratos Vegetais/análise
Folhas de Planta/química
Folhas de Planta/metabolismo
Espectrometria por Raios X
Espectrofotometria Ultravioleta
Espectroscopia de Infravermelho com Transformada de Fourier
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrobenzenes); 0 (Plant Extracts); 3M4G523W1G (Silver); TDQ283MPCW (Eosine Yellowish-(YS))
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170424
[St] Status:MEDLINE


  7 / 291 MEDLINE  
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[PMID]:28412808
[Au] Autor:Lin X; Gao X; Chen Z; Zhang Y; Luo W; Li X; Li B
[Ad] Endereço:College of Food Science, South China Agricultural University , 483 Wushan Street, Tianhe District, Guangzhou 510642, People's Republic of China.
[Ti] Título:Spontaneously Assembled Nano-aggregates in Clear Green Tea Infusions from Camellia ptilophylla and Camellia sinensis.
[So] Source:J Agric Food Chem;65(18):3757-3766, 2017 May 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tea nano-aggregates spontaneously assembled in clear tea infusions are considered as the precursors of tea cream, although their molecular basis remains obscure. Here, we characterized nano-aggregates in green tea infusions from Camellia ptilophylla, a peculiar tea variety with 6.0% of theobromine, and Camellia sinensis as the control for comparative purpose. Numerous negatively charged spherical colloidal particles of 50-100 nm in diameter were primarily found in both green tea infusions. Catechins, proteins, and carbohydrates were confirmed as the dominant components in green tea nano-aggregates. In addition, iron, copper, nickel, proteins, and gallated catechins exhibited higher aggregating affinity than other components, whereas methylxanthines and calcium contributed to the transformation of nano-aggregates into tea cream. Green tea nano-aggregates were partly destroyed by simulated gastrointestinal digestion, and removing theses peculiar particles dramatically attenuated the bioaccessibility of methylxanthines, theanine, and some catechin monomers in green tea infusions. This study enhanced our knowledge of molecular interactions in the formation of green tea cream and provided insight into physicochemical profiles, phytochemical nature, and functional effects of green tea nano-aggregates.
[Mh] Termos MeSH primário: Camellia sinensis/química
Camellia/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Catequina/química
Nanopartículas/química
Folhas de Planta/química
Xantinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Xanthines); 28109-92-4 (methylxanthine); 8R1V1STN48 (Catechin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00068


  8 / 291 MEDLINE  
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[PMID]:28386551
[Au] Autor:Wang J; Wu B; Yin H; Fan Z; Li X; Ni S; He L; Li J
[Ad] Endereço:Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Zhejiang Provincial Key Laboratory of Tree Breeding, Hangzhou, Zhejiang 311400, China; Lianyungang Academy of Agricultural Sciences, Flower Research Center, Lianyungang, Jiangsu 222000, China.
[Ti] Título:Overexpression of Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco.
[So] Source:Biomed Res Int;2017:4049534, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ascorbate peroxidase (APX) acts indispensably in synthesizing L-ascorbate (AsA) which is pivotal to plant stress tolerance by detoxifying reactive oxygen species (ROS). Enhanced activity of APX has been shown to be a key step for genetic engineering of improving plant tolerance. However it needs a deeper understanding on the maintenance of cellular ROS homeostasis in response to stress. In this study, we identified and characterized an ( ) gene from . Quantitative real-time PCR (qRT-PCR) analysis showed that was expressed in all tissues and peaked in immature green fruits; the expression levels were significantly upregulated upon cold and hot stresses. Transgenic plants displayed marked enhancements of tolerance under both cold and heat treatments, and plant growth was correlated with expression levels. Furthermore, we monitored the activities of several ROS-scavenging enzymes including , , , and , and we showed that stress tolerance was synchronized with elevated activities of ROS-scavenging. Moreover, gene expression analysis of ROS-scavenging enzymes revealed a role of to orchestrate ROS signaling in response to temperature stresses. Overall, this study presents a comprehensive characterization of cellular response related to expression and provides insights to breed crops with high temperature tolerances.
[Mh] Termos MeSH primário: Ascorbato Peroxidases/genética
Espécies Reativas de Oxigênio/metabolismo
Estresse Fisiológico/genética
Tabaco/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
Ascorbato Peroxidases/biossíntese
Camellia/enzimologia
Camellia/genética
Catalase/biossíntese
Temperatura Baixa
Regulação da Expressão Gênica de Plantas
Glutationa Transferase/biossíntese
NADH NADPH Oxirredutases/biossíntese
Oxigênio/metabolismo
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Superóxido Dismutase-1/biossíntese
Termotolerância/genética
Tabaco/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reactive Oxygen Species); EC 1.11.1.11 (Ascorbate Peroxidases); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase-1); EC 1.6.- (NADH, NADPH Oxidoreductases); EC 1.6.5.4 (monodehydroascorbate reductase (NADH)); EC 2.5.1.18 (Glutathione Transferase); S88TT14065 (Oxygen)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1155/2017/4049534


  9 / 291 MEDLINE  
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[PMID]:28298377
[Au] Autor:Sun SG; Huang ZH; Chen ZB; Huang SQ
[Ad] Endereço:Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, China.
[Ti] Título:Nectar properties and the role of sunbirds as pollinators of the golden-flowered tea ( ).
[So] Source:Am J Bot;104(3):468-476, 2017 Mar.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Properties of floral nectar have been used to predict if a plant species is pollinated by birds. To see whether winter-flowering plants evolve nectar properties corresponding to bird pollinators, nectar properties of several species (including the golden-flowered tea), as well as the role of floral visitors as effective pollinators, were examined. METHODS: Potential pollinators of were identified at different times of day and under various weather conditions. A bird exclusion experiment was used to compare the pollination effectiveness of birds and insects. Nectar sugar components (fructose, glucose, and sucrose) from growing wild and another seven species and 22 additional cultivars (all in cultivation) were examined by high-performance liquid chromatography (HPLC). KEY RESULTS: The sunbird and honeybees were the most frequent floral visitors to . Honeybee visits were significantly reduced in cloudy/rainy weather. The fruit and seed set of flowers with birds excluded were reduced by 64%, indicating that bird pollination is significant. For the wild populations of , a bagged flower could secrete 157 µL nectar; this nectar has a low sugar concentration (19%) and is sucrose-dominant (87%). The eight species and 22 cultivars had an average sugar concentration of around 30% and a sucrose concentration of 80%, demonstrating sucrose-dominant nectar in species. CONCLUSIONS: The nectar sugar composition of species was characterized by sucrose dominance. In addition, the large reduction in seed set when birds are excluded in the golden-flowered tea also supports the suggestion that these winter-flowering plants may have evolved with birds as significant pollinators.
[Mh] Termos MeSH primário: Camellia/fisiologia
Flores/fisiologia
Passeriformes/fisiologia
Néctar de Plantas/fisiologia
[Mh] Termos MeSH secundário: Animais
Abelhas/fisiologia
Camellia/química
Flores/química
Frutose/análise
Glucose/análise
Insetos/fisiologia
Néctar de Plantas/química
Polinização
Sementes/química
Sementes/fisiologia
Sacarose/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Nectar); 30237-26-4 (Fructose); 57-50-1 (Sucrose); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600428


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[PMID]:28245300
[Au] Autor:Lee HS; Choi JH; Cui L; Li Y; Yang JM; Yun JJ; Jung JE; Choi W; Yoon KC
[Ad] Endereço:Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, South Korea.
[Ti] Título:Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study.
[So] Source:Invest Ophthalmol Vis Sci;58(2):1196-1207, 2017 Feb 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To analyze the anti-inflammatory and antioxidative effects of Camellia japonica (CJ) on human corneal epithelial (HCE) cells and its therapeutic effects in a mouse model of experimental dry eye (EDE). Methods: Camellia japonica extracts of varying concentrations (0.001%, 0.01%, and 0.1%) were used to treat HCE cells. Dichlorofluorescein diacetate (DCF-DA) and dihydroethidium (DHE) assays were performed. The production of peroxiredoxin (PRX) 1-6 and manganese-dependent superoxide dismutase (MnSOD) in HCE cells was assessed using Western blot analysis. Furthermore, eye drops containing 0.001%, 0.01%, or 0.1% CJ extract or a balanced salt solution (BSS) were applied to the EDE. Clinical parameters were measured 7 days after treatment. The levels of inflammatory markers and intracellular reactive oxygen species (ROS) were measured. Results: Treatment with 0.01% and 0.1% CJ extracts decreased apoptosis in HCE cells. In addition, band intensities of PRX 1, 4, and 5, as well as MnSOD, after hydrogen peroxide (H2O2) treatment showed a significant improvement after pretreatment with 0.01% and 0.1% CJ extracts. Mice treated with 0.1% CJ extract showed significantly improved clinical parameters when compared to those of the EDE control and BSS groups. A significant decrease in the levels of inflammatory markers and intracellular ROS was observed in the 0.01% and 0.1% CJ extract groups. Conclusions: Camellia japonica extracts promoted antioxidative protein expression and suppressed apoptosis in HCE cells. Furthermore, CJ extracts improved clinical signs of dry eye and reduced oxidative stress and the expression of inflammatory markers, suggesting that eye drops containing CJ extract could be used as an adjunctive treatment for dry eye.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Camellia
Síndromes do Olho Seco/tratamento farmacológico
Células Epiteliais/efeitos dos fármacos
Epitélio Anterior/efeitos dos fármacos
Soluções Oftálmicas/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/uso terapêutico
Antioxidantes/uso terapêutico
Western Blotting
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Modelos Animais de Doenças
Síndromes do Olho Seco/metabolismo
Células Epiteliais/metabolismo
Epitélio Anterior/metabolismo
Seres Humanos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Camundongos
Soluções Oftálmicas/uso terapêutico
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/uso terapêutico
Reação em Cadeia da Polimerase
Espécies Reativas de Oxigênio/metabolismo
Lágrimas/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Ophthalmic Solutions); 0 (Plant Extracts); 0 (Reactive Oxygen Species); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-20634



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