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[PMID]:27007356
[Au] Autor:Macedo TB; Elias ST; Torres HM; Yamamoto-Silva FP; Silveira D; Magalhães PO; Lofrano-Porto A; Guerra EN; Silva MA
[Ad] Endereço:School of Dentistry, Universidade Federal de Goiás, Goiânia, GO, Brazil.
[Ti] Título:Cytotoxic Effect of Erythroxylum suberosum Combined with Radiotherapy in Head and Neck Cancer Cell Lines.
[So] Source:Braz Dent J;27(1):108-12, 2016 Jan-Feb.
[Is] ISSN:1806-4760
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The mouth and oropharynx cancer is the 6th most common type of cancer in the world. The treatment may involve surgery, chemotherapy and radiotherapy. More than 50% of drugs against cancer were isolated from natural sources, such as Catharanthus roseus and epipodophyllotoxin, isolated from Podophyllum. The biggest challenge is to maximize the control of the disease, while minimizing morbidity and toxicity to the surrounding normal tissues. The Erythroxylum suberosum is a common plant in the Brazilian Cerrado biome and is popularly known as "cabelo-de-negro". The objective of this study was to evaluate the cytotoxic activity of Erythroxylum suberosum plant extracts of the Brazilian Cerrado biome associated with radiotherapy in human cell lines of oral and hypopharynx carcinomas. Cells were treated with aqueous, ethanolic and hexanic extracts of Erythroxylum suberosum and irradiated at 4 Gy, 6 Gy and 8 Gy. Cytotoxicity was evaluated by MTT assay and the absorbance was measured at 570 nm in a Beckman Counter reader. Cisplatin, standard chemotherapy, was used as positive control. The use of Erythroxylum suberosum extracts showed a possible radiosensitizing effect in vitro for head and neck cancer. The cytotoxicity effect in the cell lines was not selective and it is very similar to the effect of standard chemotherapy. The aqueous extract of Erythroxylum suberosum, combined with radiotherapy was the most cytotoxic extract to oral and hypopharynx carcinomas.
[Mh] Termos MeSH primário: Antineoplásicos/uso terapêutico
Erythroxylaceae/química
Neoplasias de Cabeça e Pescoço/tratamento farmacológico
Neoplasias de Cabeça e Pescoço/radioterapia
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Terapia Combinada
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:160324
[St] Status:MEDLINE


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[PMID]:26918580
[Au] Autor:Elias ST; Macedo CC; Simeoni LA; Silveira D; Magalhães PO; Lofrano-Porto A; Coletta RD; Neves FA; Guerra EN
[Ad] Endereço:a Faculty of Health Sciences, University of Brasília , Brasília , Brazil.
[Ti] Título:Cytotoxic effect of Erythroxylum daphnites extract is associated with G1 cell cycle arrest and apoptosis in oral squamous cell carcinoma.
[So] Source:Cell Cycle;15(7):948-56, 2016.
[Is] ISSN:1551-4005
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant-derived molecules showing antineoplastic effects have recently gained increased attention as potential adjuvants to traditional therapies for various cancers. Cerrado biome in Brazil contains high floral biodiversity, but knowledge about the potential therapeutic effects of compounds derived from that flora is still limited. The present study investigated the antineoplastic activity of Erythroxylum daphnites Mart., a Brazilian native plant from Cerrado biome, in the SCC-9 oral squamous cell carcinoma cell line. Cells were treated with various concentrations of hexane extract of Erythroxylum daphnites leaves (EDH) and assessed for cytotoxicity, proliferation, and apoptosis. Thin layer chromatography was conducted to characterize the substances present in EDH. Our results showed that EDH exerted anti-proliferative effects in SCC-9 cells by stabilizing the cell cycle at G1 phase in association with reduced intracellular levels of cyclins D and E and increased level of p21. EDH also demonstrated pro-apoptotic properties, as shown by an increased expression of caspase-3. Triterpenes were the major constituents of EDH. Our findings demonstrated a cytotoxic effect of EDH against SCC-9 cells in vitro mediated by the restraint of cellular proliferation and induction of apoptosis. Taken together, these findings support EDH constituents as potential therapeutic adjuvants for oral cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose
Carcinoma de Células Escamosas/metabolismo
Erythroxylaceae/química
Pontos de Checagem da Fase G1 do Ciclo Celular
Neoplasias Bucais/metabolismo
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Carcinoma de Células Escamosas/patologia
Proteínas de Ciclo Celular/metabolismo
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Citotoxinas/química
Citotoxinas/farmacologia
Seres Humanos
Neoplasias Bucais/patologia
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Cell Cycle Proteins); 0 (Cytotoxins); 0 (Plant Extracts); 0 (Triterpenes)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160227
[St] Status:MEDLINE
[do] DOI:10.1080/15384101.2016.1151583


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[PMID]:26888377
[Au] Autor:Cruz RA; Almeida H; Fernandes CP; Joseph-Nathan P; Rocha L; Leitão GG
[Ad] Endereço:Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
[Ti] Título:A new tropane alkaloid from the leaves of Erythroxylum subsessile isolated by pH-zone-refining counter-current chromatography.
[So] Source:J Sep Sci;39(7):1273-7, 2016 Apr.
[Is] ISSN:1615-9314
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Tropane alkaloids are bioactive metabolites with great importance in the pharmaceutical industry and the most important class of natural products found in the Erythroxylum genus. However, these compounds are usually separated by traditional chromatographic techniques, in which the sample is progressively purified in multiple chromatographic steps, resulting in a time- and solvent-consuming procedure. In this work we present the isolation of a novel alkaloid, 6ß,7ß-dibenzoyloxytropan-3α-ol, together with the two known 3α-benzoyloxynortropan-6ß-ol and 3α,6ß-dibenzoyloxytropane alkaloids, directly from the crude alkaloid fraction from the leaves of Erythroxylum subsessile, by using a single run pH-zone-refining counter-current chromatography method. The ethyl acetate/water (1:1, v/v) biphasic solvent system with triethylamine and HCl as retention and eluter agents, respectively, was used to isolate tropane alkaloids for the first time. The structures of the isolated alkaloids were elucidated by spectroscopic methods.
[Mh] Termos MeSH primário: Distribuição Contracorrente/métodos
Erythroxylaceae/química
Folhas de Planta/química
Tropanos/isolamento & purificação
[Mh] Termos MeSH secundário: Concentração de Íons de Hidrogênio
Estrutura Molecular
Tropanos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Tropanes)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160219
[St] Status:MEDLINE
[do] DOI:10.1002/jssc.201500952


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[PMID]:26619941
[Au] Autor:Santos KC; Monte AP; Lima JT; Ribeiro LA; Palheta Junior RC
[Ad] Endereço:Course of Natural Resources in Semiarid.
[Ti] Título:Role of NO-cGMP pathway in ovine cervical relaxation induced by Erythroxylum caatingae Plowman.
[So] Source:Anim Reprod Sci;164:23-30, 2016 Jan.
[Is] ISSN:1873-2232
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Erythroxylum caatingae Plowman has a myorelaxing effect on smooth muscle tissue. We investigated the effect of the crude ethanolic extract of E. caatingae Plowman (Ec-EtOH) on the contractility of the ovine cervix. In an isometric system, circular strips were subjected to 90mM potassium (K(+)) or 30µM carbamylcholine (CCh)-induced contraction. We then exposed the tissue to cumulative concentrations of Ec-EtOH (1-729 µg/ml). In other bath solutions, the tissues were exposed to l-NG-nitroarginine methyl ester (l-NAME; 100µM), l-NAME (100µM)+l-arginine (300µM), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, ODQ; 5µM), 4-aminopyridine (4-AP; 3mM), tetraethylammonium (TEA; 0.3mM), glybenclamide (1µM), atosiban (10µM) or verapamil (3µM), followed by the addition of Ec-EtOH (1-729 µg/ml). We also evaluated the effect of cervical Ec-EtOH infusion (2mg) on cervical contractility in vivo. Ec-EtOH decreased cervical contractility induced by K(+) or CCh, and 729 µg/ml Ec-EtOH decreased 85.4±5.1% the amplitude of basal contractility in vitro, with an EC50 of 17.9±3.7 µg/ml. This effect of Ec-EtOH was prevented by l-NAME or ODQ. l-arginine impaired the blunting effect of l-NAME on cervical relaxation caused by Ec-EtOH. However, the potassium channel blockers 4-AP, TEA, and glybenclamide did not modify this myorelaxation triggered by Ec-EtOH. Ec-EtOH also decreased acetylcholine-induced contractions in tissue preincubated with verapamil. In addition, Ec-EtOH decreased ovine cervical contractions in vivo. Thus, Ec-EtOH had a relaxant effect on ovine cervical contractions. This may involve the nitric oxide signal, mediated by cGMP cellular transduction, and be related to intracellular calcium sequestration.
[Mh] Termos MeSH primário: Colo do Útero/efeitos dos fármacos
GMP Cíclico/metabolismo
Erythroxylaceae/química
Óxido Nítrico/metabolismo
Extratos Vegetais/farmacologia
Ovinos
[Mh] Termos MeSH secundário: Animais
Feminino
Contração Muscular/efeitos dos fármacos
Relaxamento Muscular/efeitos dos fármacos
Músculo Liso/efeitos dos fármacos
Extratos Vegetais/química
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Extracts); 31C4KY9ESH (Nitric Oxide); H2D2X058MU (Cyclic GMP)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160102
[Lr] Data última revisão:
160102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151202
[St] Status:MEDLINE


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[PMID]:25406120
[Au] Autor:Schmidt GW; Jirschitzka J; Porta T; Reichelt M; Luck K; Torre JC; Dolke F; Varesio E; Hopfgartner G; Gershenzon J; D'Auria JC
[Ad] Endereço:Department of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne,
[Ti] Título:The last step in cocaine biosynthesis is catalyzed by a BAHD acyltransferase.
[So] Source:Plant Physiol;167(1):89-101, 2015 Jan.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The esterification of methylecgonine (2-carbomethoxy-3ß-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase. The enzyme is capable of producing both cocaine and cinnamoylcocaine via the activated benzoyl- or cinnamoyl-Coenzyme A thioesters, respectively. Cocaine synthase activity is highest in young developing leaves, especially in the palisade parenchyma and spongy mesophyll. These data correlate well with the tissue distribution pattern of cocaine as visualized with antibodies. Matrix-assisted laser-desorption ionization mass spectral imaging revealed that cocaine and cinnamoylcocaine are differently distributed on the upper versus lower leaf surfaces. Our findings provide further evidence that tropane alkaloid biosynthesis in the Erythroxylaceae occurs in the above-ground portions of the plant in contrast with the Solanaceae, in which tropane alkaloid biosynthesis occurs in the roots.
[Mh] Termos MeSH primário: Aciltransferases/metabolismo
Cocaína/biossíntese
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Catálise
Cocaína/análogos & derivados
Cocaína/análise
Erythroxylaceae/enzimologia
Erythroxylaceae/metabolismo
Células do Mesofilo/enzimologia
Células do Mesofilo/metabolismo
Folhas de Planta/enzimologia
Folhas de Planta/metabolismo
Proteínas de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); EC 2.3.- (Acyltransferases); I5Y540LHVR (Cocaine); O3I44O988U (cinnamoylcocaine)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141120
[St] Status:MEDLINE
[do] DOI:10.1104/pp.114.248187


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[PMID]:25096669
[Au] Autor:Elias ST; Borges GA; Amorim DA; Rêgo DF; Simeoni LA; Silveira D; Fonseca-Bazzo YM; Paula JE; Fagg CW; Barros IM; Abreu WC; Pinto-Júnior DS; Magalhães PO; Neves FA; Lofrano-Porto A; Guerra EN
[Ad] Endereço:Faculty of Health Sciences, University of Brasília, SQN 205, bloco H apto 201, Asa Norte, Brasilia, DF, 70843-080, Brazil.
[Ti] Título:Radiation induced a supra-additive cytotoxic effect in head and neck carcinoma cell lines when combined with plant extracts from Brazilian Cerrado biome.
[So] Source:Clin Oral Investig;19(3):637-46, 2015 Apr.
[Is] ISSN:1436-3771
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Antineoplastic effects of molecules derived from plants have recently gained increasing attention as an additive to traditional therapies. The aim of this study was to evaluate the cytotoxic activity of plant extracts from the Brazilian Cerrado biome associated with radiotherapy in head and neck carcinoma cells (HNSCC). MATERIALS AND METHODS: Fifteen extracts derived from five Cerrado plants were tested in HNSCC cell lines (SCC-25, SCC-9, FaDu) and keratinocyte cells (HaCat). Cell cytotoxicity of extracts and association extract/radiation (2Gy/min) was assessed by MTT assay. Cisplatin (50 µg/mL) was used as a positive control. Extracts with the major cytotoxic activity were selected and their IC50 concentrations were defined. Apoptosis was assessed using flow cytometric analysis. RESULTS: Ten isolated extracts resulted in moderate cytotoxicity (>20 and ≤ 50 % of viable cells), while three extracts induced severe cytotoxic effects (≤ 20 % of viable cells). Plant extracts treatment improved radiotherapy cytotoxicity in all cell lines. Although plant extracts are not as potent as cisplatin plus radiation, in FaDu cells, seven extracts associated with irradiation showed cytotoxic activity similar or better than the association of cisplatin and radiation. Hexanic extract of Erythroxylum daphinites could induce apoptosis in oral cancer cells; however, necrosis was the prevalent kind of death in FaDu cells treated with hexanic extract of Erythroxylum suberosum. CONCLUSIONS: Pre-treatment of HNSCC cells with the extract derived from Cerrado plants followed by irradiation induced a supra-additive cytotoxic effect. CLINICAL RELEVANCE: This study highlights the potential biological relevance of the Cerrado biome when associated with traditional therapy for cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Carcinoma de Células Escamosas/tratamento farmacológico
Carcinoma de Células Escamosas/radioterapia
Neoplasias de Cabeça e Pescoço/tratamento farmacológico
Neoplasias de Cabeça e Pescoço/radioterapia
Extratos Vegetais/farmacologia
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Apoptose/efeitos da radiação
Brasil
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Erythroxylaceae
Citometria de Fluxo
Seres Humanos
Queratinócitos/efeitos dos fármacos
Medicina Tradicional
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:140807
[St] Status:MEDLINE
[do] DOI:10.1007/s00784-014-1289-z


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[PMID]:25918783
[Au] Autor:Menezes-Filho Nde J; Souza Cdos S; Costa TC; Da Silva VD; Ribeiro CS; Barreiros ML; Costa JF; David JM; David JP; Costa SL
[Ti] Título:Cytotoxicity of the diterpene 14-O-methyl-ryanodanol from Erythroxylum passerinum in an astrocytic cells model.
[So] Source:Nat Prod Commun;9(9):1245-8, 2014 Sep.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant secondary metabolites, such as, specifically, alkaloids and terpenes, may present psychoactive properties that modify the function of the central nervous system (CNS) and induce neurotoxicity. Neurotoxicity involves the response of glial cells, mainly astrocytes, which play a fundamental role in the control of homeostasis of the CNS. Some Erythroxylum species are indigenous to the state of Bahia in Brazil. This study investigated the cytotoxic activity of the diterpene AEP-1, extracted from the fruit of E. passerinum in a GL-15 cell line, astrocytic, glial cells model. The effects on cell viability, analyzed by the MTT assay, demonstrated a dose-dependent cytotoxic effect, with maximum effect at 500 µg/mL of AEP-1, and with a reduction of about 40 and 47% on cellular viability after 24 h and 72 h treatment, respectively. Evidence for induction of apoptosis by AEP-1 was first obtained when GL-15 glial cells were incubated with 250 µg/mL AEP-1 causing reniform and/or pyknotic nuclei and apoptotic bodies revealed by chromatin staining with Hoechst 33258. Increase in DNA fragmentation was also observed by comet assays in cells incubated with 500 µg/mL of AEP-1. Moreover, cells exposed to a sub toxic dose of AEP-1 (250 µg/mL) showed significant changes in morphology--contraction of the cytoplasm and expansion of cellular projections--signifying the presence of astrocytic cytoskeletal protein and glial fibrillary acidic protein (GFAP). These findings indicated astrocytic cells as the target for terpene AEP-1 and suggest the involvement of glial cells with psychoactive symptoms observed in humans and animals after consumption of fruits of plants of the genus Erythroxylum.
[Mh] Termos MeSH primário: Astrócitos/citologia
Astrocitoma/fisiopatologia
Diterpenos/farmacologia
Erythroxylaceae/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Astrócitos/efeitos dos fármacos
Linhagem Celular Tumoral
Sobrevivência Celular
Fragmentação do DNA/efeitos dos fármacos
Seres Humanos
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (14-O-methyl-ryanodanol); 0 (Diterpenes); 0 (Plant Extracts)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:150428
[Lr] Data última revisão:
150428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150429
[St] Status:MEDLINE


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[PMID]:24660454
[Au] Autor:Muñoz MA; Arriagada S; Joseph-Nathan P
[Ti] Título:Chiral resolution and absolute configuration of 3alpha,6beta-dicinnamoyloxytropane and 3alpha,6beta-di(1-ethyl-1H-pyrrol-2-ylcarbonyloxy)tropane, constituents of Erythroxylum species.
[So] Source:Nat Prod Commun;9(1):27-30, 2014 Jan.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chiral resolution of (+/-)-3alpha,6beta-dicinnamoyloxytropane (1) and (+/-)-3alpha,6beta-di(1-methyl-1H-pyrrol-2-ylcarbonyloxy)tropane (2), prepared by esterification of (+/-)-3alpha,6beta-tropanediol (3), was achieved using an amylose-derived HPLC stationary phase and normal phase conditions. The corresponding vibrational circular dichroism (VCD) spectra provided the absolute configuration of the enantiomers as (-)-(3R,6R)-1, (+)-(3S,6S)-1, (-)-(3R,6R)-2 and (+)-(3S,6S)-2. In each case, characteristic VCD bands for the absolute configuration determination of the 3alpha,6beta-tropandiol esters were observed. While the absolute configuration of natural 1, previously isolated from Erythroxylum hypericifolium, could not be established due to the lack of literature optical rotation values, that of catuabine E, previously isolated from E. vacciniifolium, is now assigned as (-)-(3R,6R)-2 by comparison with the optical rotation values of the prepared samples and the reported rotation of the natural product.
[Mh] Termos MeSH primário: Cinamatos/química
Erythroxylaceae/química
Tropanos/química
[Mh] Termos MeSH secundário: Dicroísmo Circular
Conformação Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (3alpha,6beta-di(1-ethyl-1H-pyrrol-2-ylcarbonyloxy)tropane); 0 (3alpha,6beta-dicinnamoyloxytropane); 0 (Cinnamates); 0 (Tropanes)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:140325
[Lr] Data última revisão:
140325
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140326
[St] Status:MEDLINE


  9 / 43 MEDLINE  
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[PMID]:24561557
[Au] Autor:Casale JF; Mallette JR; Jones LM
[Ad] Endereço:Special Testing and Research Laboratory, Drug Enforcement Administration, U.S. Department of Justice, Dulles, VA 20166-9509, United States. Electronic address: John.f.casale@usdoj.gov.
[Ti] Título:Chemosystematic identification of fifteen new cocaine-bearing Erythroxylum cultigens grown in Colombia for illicit cocaine production.
[So] Source:Forensic Sci Int;237:30-9, 2014 Apr.
[Is] ISSN:1872-6283
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Colombian coca farmers have historically cultivated three varieties of coca for cocaine production (Erythroxylum novogranatense var. novogranatense, Erythroxylum novogranatense var. truxillense, and Erythroxylum coca var. ipadu). Within the past 13 years, 15 new cultigens of cocaine-bearing Erythroxylum have been propagated by Colombian coca farmers; each with differing physical characteristics, yet producing cocaine alkaloids at similar levels found in the historical and native varieties. Fifteen new cultigens were collected from throughout Colombia and propagated along with the three historical varieties within an experimental field in Colombia. Five plants/cultigen were randomly selected and examined for alkaloid content to determine their varietal characteristics when compared to the three known varieties. Ten cultigens gave classic Erythroxylum coca var. ipadu alkaloid profiles, four cultigens produced alkaloid profiles consistent with a hybridization of Erythroxylum novogranatense and Erythroxylum coca var. ipadu, while one cultigen gave heterogeneous alkaloid profiles that could not be characterized.
[Mh] Termos MeSH primário: Cocaína/análise
Erythroxylaceae/química
Drogas Ilícitas/análise
[Mh] Termos MeSH secundário: Agricultura
Alcaloides/química
Colômbia
Erythroxylaceae/classificação
Ionização de Chama
Seres Humanos
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Street Drugs); I5Y540LHVR (Cocaine)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:140328
[Lr] Data última revisão:
140328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140225
[St] Status:MEDLINE


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[PMID]:23870471
[Au] Autor:Wesam RK; Ghanya AN; Mizaton HH; Ilham M; Aishah A
[Ad] Endereço:Faculty of Pharmacy, Universiti Teknologi MARA, Shah Alam, Selangor.
[Ti] Título:Assessment of genotoxicity and cytotoxicity of standardized aqueous extract from leaves of Erythroxylum cuneatum in human HepG2 and WRL68 cells line.
[So] Source:Asian Pac J Trop Med;6(10):811-6, 2013 Oct.
[Is] ISSN:2352-4146
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To investigate the cytotoxicity and the genotoxicity of standardized aqueous of dry leaves of Erythroxylum cuneatum (E. cuneatum) in human HepG2 and WRL68 cells. METHODS: The cytotoxicity of E. cuneatum extract was evaluated by both MTS and LDH assays. Genotoxicity study on E. cuneatum extract was assessed by the single cell gel electrophoresis (comet assay). The protective effect of E. cuneatum against menadione-induced cytotoxicity was also investigated. RESULTS: Results from this study showed that E. cuneatum extract exhibited cytotoxic activities towards the cells with IC50 value of (125±12) and (125±14) µg/mL for HepG2 and WRL68 cells respectively, after 72 h incubation period as determined by MTS assay. LDH leakage was detected at (251±19) and (199.5±12.0) µg/mL for HepG2 and WRL68 respectively. Genotoxicity study results showed that treatment with E. cuneatum up to 1 mg/mL did not cause obvious DNA damage in WRL68 and HepG2 cells. Addition of E. cunaetum did not show significant protection towards menadione in WRL68 and HepG2 Cells. CONCLUSIONS: E. cuneatum standardized aqueous extract might be developed in order to establish new pharmacological possibilities for its application.
[Mh] Termos MeSH primário: Citotoxinas/toxicidade
Erythroxylaceae/química
Mutagênicos/toxicidade
Extratos Vegetais/toxicidade
Folhas de Planta/química
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Ensaio Cometa
Dano ao DNA/efeitos dos fármacos
Células Hep G2
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytotoxins); 0 (Mutagens); 0 (Plant Extracts)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:160519
[Lr] Data última revisão:
160519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130723
[St] Status:MEDLINE



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