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[PMID]:29284007
[Au] Autor:Zhang X; Li R; Kang H; Luo D; Fan J; Zhu W; Liu X; Tong Q
[Ad] Endereço:College of Food and Bioengineering, Henan University of Science and Technology, Henan Luoyang, China.
[Ti] Título:Effects of low molecular sugars on the retrogradation of tapioca starch gels during storage.
[So] Source:PLoS One;12(12):e0190180, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effects of low molecular sugars (sucrose, glucose and trehalose) on the retrogradation of tapioca starch (TS) gels stored at 4°C for different periods were examined with different methods. Decrease in melting enthalpy (ΔHmelt) were obtained through differential scanning calorimetry analysis. Analysis of decrease in crystallization rate constant (k) and increase in semi-crystallization time (τ1/2) results obtained from retrogradation kinetics indicated that low molecular sugars could retard the retrogradation of TS gels and further revealed trehalose as the best inhibitor among the sugars used in this study. Fourier transform infrared (FTIR) analysis indicated that the intensity ratio of 1047 to 1022 cm-1 was increased with the addition of sugars in the order of trehalose > sucrose > glucose. Decrease in hardness parameters and increase in springiness parameters obtained from texture profile analysis (TPA) analysis also indicated that low molecular sugars could retard the retrogradation of TS gels. The results of FTIR and TPA showed a consistent sugar effect on starch retrogradation with those of DSC and retrogradation kinetics analysis.
[Mh] Termos MeSH primário: Manihot/química
Amido/química
Açúcares/química
[Mh] Termos MeSH secundário: Varredura Diferencial de Calorimetria
Peso Molecular
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Sugars); 9005-25-8 (Starch)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190180


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[PMID]:28742460
[Au] Autor:Garrett KA; Andersen KF; Asche F; Bowden RL; Forbes GA; Kulakow PA; Zhou B
[Ad] Endereço:First and second authors: Plant Pathology Department, Emerging Pathogens Institute, and Institute for Sustainable Food Systems, University of Florida, Gainesville 32611; third author: School of Forest Resources and Conservation and Institute for Sustainable Food Systems, University of Florida, Gaine
[Ti] Título:Resistance Genes in Global Crop Breeding Networks.
[So] Source:Phytopathology;107(10):1268-1278, 2017 10.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Resistance genes are a major tool for managing crop diseases. The networks of crop breeders who exchange resistance genes and deploy them in varieties help to determine the global landscape of resistance and epidemics, an important system for maintaining food security. These networks function as a complex adaptive system, with associated strengths and vulnerabilities, and implications for policies to support resistance gene deployment strategies. Extensions of epidemic network analysis can be used to evaluate the multilayer agricultural networks that support and influence crop breeding networks. Here, we evaluate the general structure of crop breeding networks for cassava, potato, rice, and wheat. All four are clustered due to phytosanitary and intellectual property regulations, and linked through CGIAR hubs. Cassava networks primarily include public breeding groups, whereas others are more mixed. These systems must adapt to global change in climate and land use, the emergence of new diseases, and disruptive breeding technologies. Research priorities to support policy include how best to maintain both diversity and redundancy in the roles played by individual crop breeding groups (public versus private and global versus local), and how best to manage connectivity to optimize resistance gene deployment while avoiding risks to the useful life of resistance genes. [Formula: see text] Copyright © 2017 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .
[Mh] Termos MeSH primário: Resistência à Doença/genética
Manihot/genética
Oryza/genética
Doenças das Plantas/imunologia
Solanum tuberosum/genética
Triticum/genética
[Mh] Termos MeSH secundário: Agricultura
Cruzamento
Clima
Produtos Agrícolas
Abastecimento de Alimentos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-03-17-0082-FI


  3 / 1637 MEDLINE  
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[PMID]:27778353
[Au] Autor:De Souza AP; Massenburg LN; Jaiswal D; Cheng S; Shekar R; Long SP
[Ad] Endereço:Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
[Ti] Título:Rooting for cassava: insights into photosynthesis and associated physiology as a route to improve yield potential.
[So] Source:New Phytol;213(1):50-65, 2017 Jan.
[Is] ISSN:1469-8137
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Contents 50 I. 50 II. 52 III. 54 IV. 55 V. 57 VI. 57 VII. 59 60 References 61 SUMMARY: As a consequence of an increase in world population, food demand is expected to grow by up to 110% in the next 30-35 yr. The population of sub-Saharan Africa is projected to increase by > 120%. In this region, cassava (Manihot esculenta) is the second most important source of calories and contributes c. 30% of the daily calorie requirements per person. Despite its importance, the average yield of cassava in Africa has not increased significantly since 1961. An evaluation of modern cultivars of cassava showed that the interception efficiency (É› ) of photosynthetically active radiation (PAR) and the efficiency of conversion of that intercepted PAR (É› ) are major opportunities for genetic improvement of the yield potential. This review examines what is known of the physiological processes underlying productivity in cassava and seeks to provide some strategies and directions toward yield improvement through genetic alterations to physiology to increase É› and É› . Possible physiological limitations, as well as environmental constraints, are discussed.
[Mh] Termos MeSH primário: Manihot/crescimento & desenvolvimento
Manihot/fisiologia
Fotossíntese
[Mh] Termos MeSH secundário: Meio Ambiente
Manihot/genética
Folhas de Planta/fisiologia
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1111/nph.14250


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[PMID]:29228026
[Au] Autor:Ikeogu UN; Davrieux F; Dufour D; Ceballos H; Egesi CN; Jannink JL
[Ad] Endereço:Plant Breeding and Genetics Section, Cornell University, Ithaca, NY, United States of America.
[Ti] Título:Rapid analyses of dry matter content and carotenoids in fresh cassava roots using a portable visible and near infrared spectrometer (Vis/NIRS).
[So] Source:PLoS One;12(12):e0188918, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Portable Vis/NIRS are flexible tools for fast and unbiased analyses of constituents with minimal sample preparation. This study developed calibration models for dry matter content (DMC) and carotenoids in fresh cassava roots using a portable Vis/NIRS system. We examined the effects of eight data pre-treatment combinations on calibration models and assessed calibrations on processed and intact root samples. We compared Vis/NIRS derived-DMC to other phenotyping methods. The results of the study showed that the combination of standard normal variate and de-trend (SNVD) with first derivative calculated on two data points and no smoothing (SNVD+1111) was adequate for a robust model. Calibration performance was higher with processed than the intact root samples for all the traits although intact root models for some traits especially total carotenoid content (TCC) (R2c = 96%, R2cv = 90%, RPD = 3.6 and SECV = 0.63) were sufficient for screening purposes. Using three key quality traits as templates, we developed models with processed fresh root samples. Robust calibrations were established for DMC (R2c = 99%, R2cv = 95%, RPD = 4.5 and SECV = 0.9), TCC (R2c = 99%, R2cv = 91%, RPD = 3.5 and SECV = 2.1) and all Trans ß-carotene (ATBC) (R2c = 98%, R2cv = 91%, RPD = 3.5 and SECV = 1.6). Coefficient of determination on independent validation set (R2p) for these traits were also satisfactory for ATBC (91%), TCC (88%) and DMC (80%). Compared to other methods, Vis/NIRS-derived DMC from both intact and processed roots had very high correlation (>0.95) with the ideal oven-drying than from specific gravity method (0.49). There was equally a high correlation (0.94) between the intact and processed Vis/NIRS DMC. Therefore, the portable Vis/NIRS could be employed for the rapid analyses of DMC and quantification of carotenoids in cassava for nutritional and breeding purposes.
[Mh] Termos MeSH primário: Carotenoides/análise
Manihot/química
Raízes de Plantas/química
Espectroscopia de Luz Próxima ao Infravermelho/métodos
[Mh] Termos MeSH secundário: Calibragem
Modelos Químicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
36-88-4 (Carotenoids)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188918


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[PMID]:29227875
[Au] Autor:Doyi INY; Essumang DK; Agyapong AK; Asumadu-Sarkodie S
[Ad] Endereço:Radioactive Waste Management Centre, Ghana Atomic Energy Commission, P. O. Box LG 80, Legon, Accra, Ghana; Department of Environmental Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia. Electronic address: i.doyi@gaecgh.org.
[Ti] Título:Soil-to-cassava transfer of naturally occurring radionuclides from communities along Ghana's oil and gas rich Tano Basin.
[So] Source:J Environ Radioact;182:138-141, 2018 Feb.
[Is] ISSN:1879-1700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Soil-to-plant transfer factor (TF) is widely used to assess the impact of soil radioactivity on agricultural crops. The root crop cassava (Manihot esculenta) provides 30%-50% of the calories consumed in Sub-Saharan Africa and is widely used in South America. γ-ray analysis was used to measure activity concentrations of U, Th, and K in cassava root and soil. The TF values for U, Th, and K were in the range 0.06-0.12, 0.01-0.10 and 0.04-0.28 respectively. The median transfer factors were 0.10 ( U), 0.04 ( Th) and 0.08 ( K). For U and Th, the highest TF values were 0.12 and 0.10 respectively.
[Mh] Termos MeSH primário: Manihot/química
Monitoramento de Radiação
Poluentes Radioativos do Solo/análise
[Mh] Termos MeSH secundário: Radiação de Fundo
Gana
Radioatividade
Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 0 (Soil Pollutants, Radioactive)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE


  6 / 1637 MEDLINE  
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[PMID]:29190504
[Au] Autor:Yoodee S; Kobayashi Y; Songnuan W; Boonchird C; Thitamadee S; Kobayashi I; Narangajavana J
[Ad] Endereço:Department of Biotechnology, Faculty of Science, Mahidol University, Phayathai, Bangkok, Thailand.
[Ti] Título:Phytohormone priming elevates the accumulation of defense-related gene transcripts and enhances bacterial blight disease resistance in cassava.
[So] Source:Plant Physiol Biochem;122:65-77, 2018 Jan.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Cassava bacterial blight (CBB) disease caused by Xanthomonas axonopodis pv. manihotis (Xam) is a severe disease in cassava worldwide. In addition to causing significant cassava yield loss, CBB disease has not been extensively studied, especially in terms of CBB resistance genes. The present research demonstrated the molecular mechanisms underlining the defense response during Xam infection in two cassava cultivars exhibiting different degrees of disease resistance, Huay Bong60 (HB60) and Hanatee (HN). Based on gene expression analysis, ten of twelve putative defense-related genes including, leucine-rich repeat receptor-like kinases (LRR-RLKs), resistance (R), WRKY and pathogenesis-related (PR) genes, were differentially expressed between these two cassava cultivars during Xam infection. The up-regulation of defense-related genes observed in HB60 may be the mechanism required for the reduction of disease severity in the resistant cultivar. Interestingly, priming with salicylic acid (SA) or methyl jasmonate (MeJA) for 24 h before Xam inoculation could enhance the defense response in both cassava cultivars. The disease severity was decreased 10% in the resistant cultivar (HB60) and was remarkably reduced 21% in the susceptible cultivar (HN) by SA/MeJA priming. Priming with Xam inoculation modulated cassava4.1_013417, cassava4.1_030866 and cassava4.1_020555 (highest similarity to MeWRKY59, MePR1 and AtPDF2.2, respectively) expression and led to enhanced resistance of the susceptible cultivar in the second infection. The putative cis-regulatory elements were predicted in an upstream region of these three defense-related genes. The different gene expression levels in these genes between the two cultivars were due to the differences in cis-regulatory elements in their promoter regions. Taken together, our study strongly suggested that the induction of defense-related genes correlated with defense resistance against Xam infection, and exogenous application of SA or MeJA could elevate the defense response in both cultivars of cassava. This finding should pave the way for management to reduce yield loss from disease and genetic improvement in cassava.
[Mh] Termos MeSH primário: Resistência à Doença/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Manihot
Fitocromo/farmacologia
Doenças das Plantas/microbiologia
Transcrição Genética/efeitos dos fármacos
Xanthomonas axonopodis/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Manihot/metabolismo
Manihot/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
11121-56-5 (Phytochrome)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


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[PMID]:29056507
[Au] Autor:Yan Y; Wang P; He C; Shi H
[Ad] Endereço:Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources and College of Biology, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China.
[Ti] Título:MeWRKY20 and its interacting and activating autophagy-related protein 8 (MeATG8) regulate plant disease resistance in cassava.
[So] Source:Biochem Biophys Res Commun;494(1-2):20-26, 2017 Dec 09.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As a highly conserved mechanism, autophagy is responsible for the transport of cytoplasmic constituents in the vacuoles or lysosomes. Moreover, autophagy is essential for plant development and various stress responses. In this study, 34 MeATGs were systematically identified in cassava, and their transcripts were commonly regulated by Xanthomonas axonopodis pv manihotis (Xam). Through transient expression in Nicotiana benthamiana, the subcellular locations of 4 MeATG8s were revealed. Notably, MeWRKY20 was identified as physical interacting protein of MeATG8a/8f/8h and upstream transcriptional activator of MeATG8a. Through virus-induced gene silencing (VIGS) in cassava, we found that MeATG8-silenced and MeWRKY20-silenced plants resulted in disease sensitive, with less callose depositions and lower autophagic activity. This study may facilitate our understanding of the upstream MeWRKY20 and underlying target as well as interacting proteins of MeATG8s in immune response. Taken together, MeWRKY20 and MeATG8a/8f/8h are essential for disease resistance against bacterial blight by forming various transcriptional modules and interacting complex in cassava.
[Mh] Termos MeSH primário: Família da Proteína 8 Relacionada à Autofagia/fisiologia
Manihot/fisiologia
Proteínas de Plantas/fisiologia
[Mh] Termos MeSH secundário: Autofagia/genética
Autofagia/fisiologia
Família da Proteína 8 Relacionada à Autofagia/genética
Família da Proteína 8 Relacionada à Autofagia/imunologia
Genes de Plantas
Manihot/genética
Manihot/microbiologia
Doenças das Plantas/genética
Doenças das Plantas/imunologia
Doenças das Plantas/microbiologia
Proteínas de Plantas/genética
Proteínas de Plantas/imunologia
Plantas Geneticamente Modificadas
Tabaco/genética
Transativadores/genética
Transativadores/imunologia
Transativadores/fisiologia
Xanthomonas axonopodis/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Autophagy-Related Protein 8 Family); 0 (Plant Proteins); 0 (Trans-Activators)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171024
[St] Status:MEDLINE


  8 / 1637 MEDLINE  
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[PMID]:28813463
[Au] Autor:Tocko-Marabena BK; Silla S; Simiand C; Zinga I; Legg J; Reynaud B; Delatte H
[Ad] Endereço:Laboratoire des Sciences Biologiques et Agronomique pour le Développement (LBSAD), Université de Bangui, Bangui, Centrafrique.
[Ti] Título:Genetic diversity of Bemisia tabaci species colonizing cassava in Central African Republic characterized by analysis of cytochrome c oxidase subunit I.
[So] Source:PLoS One;12(8):e0182749, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:After 2007, upsurges of whiteflies on cassava plants and high incidences of cassava diseases were observed in Central African Republic. This recent upsurge in the abundance of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) was directly linked to serious damage to cassava crops resulting from spread of whitefly-borne cassava mosaic geminiviruses (CMGs). There is currently very little information describing whitefly populations on cassava and associated crops in Central African Republic. The current study aimed to address this gap, and to determine whether the increasing damage associated with B. tabaci whiteflies was the consequence of a new invasion, or an upsurge of a local population. The molecular genetic identification and phylogenetic relationships of 898 B. tabaci adult individuals collected from representative locations (54) throughout CAR were determined based on their mitochondrial cytochrome oxidase I sequences (mtCOI). Field and ecological data were also collected from each site, including whitefly abundance, CMD incidence, host plants colonized by B. tabaci and agro-ecological zone. Phylogenetic analysis of the whitefly mtCOI sequences indicated that SSA1 (-SG1, -SG2), SSA3, MED, MEAM1 and Indian Ocean (IO) putative species occur in CAR. One specific haplotype of SSA1-SG1 (SSA1-SG1-P18F5) predominated on most cassava plants and at the majority of sites. This haplotype was identical to the SSA1-SG1 Mukono8-4 (KM377961) haplotype that was recorded from Uganda but that also occurs widely in CMD pandemic-affected areas of East Africa. These results suggest that the SSA1-SG1-P18F5 haplotype occurring in CAR represents a recent invasive population, and that it is the likely cause of the increased spread and severity of CMD in CAR. Furthermore, the high mtDNA sequence diversity observed for SSA1 and its broad presence on all sites and host plants sampled suggest that this genetic group was the dominant resident species even before the arrival of this new invasive haplotype.
[Mh] Termos MeSH primário: Complexo IV da Cadeia de Transporte de Elétrons/genética
Variação Genética/genética
Hemípteros/classificação
Hemípteros/genética
Manihot/virologia
[Mh] Termos MeSH secundário: Animais
Begomovirus/fisiologia
República Centro-Africana
Produtos Agrícolas/virologia
Complexo IV da Cadeia de Transporte de Elétrons/química
Complexo IV da Cadeia de Transporte de Elétrons/metabolismo
Haplótipos
Hemípteros/virologia
Filogenia
Doenças das Plantas/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182749


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[PMID]:28806727
[Au] Autor:Utsumi Y; Utsumi C; Tanaka M; Ha VT; Matsui A; Takahashi S; Seki M
[Ad] Endereço:Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, Japan.
[Ti] Título:Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.
[So] Source:PLoS One;12(8):e0180736, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Agrobacterium-mediated transformation is an important research tool for the genetic improvement of cassava. The induction of friable embryogenic callus (FEC) is considered as a key step in cassava transformation. In the present study, the media composition was optimized for enhancing the FEC induction, and the effect of the optimized medium on gene expression was evaluated. In relative comparison to MS medium, results demonstrated that using a medium with reducing nutrition (a 10-fold less concentration of nitrogen, potassium, and phosphate), the increased amount of vitamin B1 (10 mg/L) and the use of picrolam led to reprogram non-FEC to FEC. Gene expression analyses revealed that FEC on modified media increased the expression of genes related to the regulation of polysaccharide biosynthesis and breakdown of cell wall components in comparison to FEC on normal CIM media, whereas the gene expression associated with energy flux was not dramatically altered. It is hypothesized that we reprogram non-FEC to FEC under low nitrogen, potassium and phosphate and high vitamin B1. These findings were more effective in inducing FEC formation than the previous protocol. It might contribute to development of an efficient transformation strategy in cassava.
[Mh] Termos MeSH primário: Manihot/embriologia
Nitratos/farmacologia
Fosfatos/farmacologia
Técnicas de Embriogênese Somática de Plantas
Potássio/farmacologia
[Mh] Termos MeSH secundário: Meios de Cultura
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Genes de Plantas
Genótipo
Manihot/efeitos dos fármacos
Manihot/genética
Análise de Sequência com Séries de Oligonucleotídeos
Plantas Geneticamente Modificadas
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Tiamina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Nitrates); 0 (Phosphates); 0 (RNA, Messenger); RWP5GA015D (Potassium); X66NSO3N35 (Thiamine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180736


  10 / 1637 MEDLINE  
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[PMID]:28771520
[Au] Autor:Shi S; Zhang X; Mandel MA; Zhang P; Zhang Y; Ferguson M; Amuge T; Rounsley S; Liu Z; Xiong Z
[Ad] Endereço:Institute of Tropical biology and biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
[Ti] Título:Variations of five eIF4E genes across cassava accessions exhibiting tolerant and susceptible responses to cassava brown streak disease.
[So] Source:PLoS One;12(8):e0181998, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cassava (Manihot esculenta) is an important tropical subsistence crop that is severely affected by cassava brown streak disease (CBSD) in East Africa. The disease is caused by Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Both have a (+)-sense single-stranded RNA genome with a 5' covalently-linked viral protein, which functionally resembles the cap structure of mRNA, binds to eukaryotic translation initiation factor 4E (eIF4E) or its analogues, and then enable the translation of viral genomic RNA in host cells. To characterize cassava eIF4Es and their potential role in CBSD tolerance and susceptibility, we cloned five eIF4E transcripts from cassava (accession TMS60444). Sequence analysis indicated that the cassava eIF4E family of proteins consisted of one eIF4E, two eIF(iso)4E, and two divergent copies of novel cap-binding proteins (nCBPs). Our data demonstrated experimentally the coding of these five genes as annotated in the published cassava genome and provided additional evidence for refined annotations. Illumina resequencing data of the five eIF4E genes were analyzed from 14 cassava lines tolerant or susceptible to CBSD. Abundant single nucleotide polymorphisms (SNP) and biallelic variations were observed in the eIF4E genes; however, most of the SNPs were located in the introns and non-coding regions of the exons. Association studies of non-synonymous SNPs revealed no significant association between any SNP of the five eIF4E genes and the tolerance or susceptibility to CBSD. However, two SNPs in two genes were weakly associated with the CBSD responses but had no direct causal-effect relationship. SNPs in an intergenic region upstream of eIF4E_me showed a surprising strong association with CBSD responses. Digital expression profile analysis showed differential expression of different eIF4E genes but no significant difference in gene expression was found between susceptible and tolerant cassava accessions despite the association of the intergenic SNPs with CBSD responses.
[Mh] Termos MeSH primário: Resistência à Doença/imunologia
Fator de Iniciação 4E em Eucariotos/genética
Variação Genética/genética
Manihot/imunologia
Doenças das Plantas/imunologia
Plantas Geneticamente Modificadas/imunologia
Potyviridae/fisiologia
[Mh] Termos MeSH secundário: Resistência à Doença/genética
Interações Hospedeiro-Patógeno
Manihot/crescimento & desenvolvimento
Manihot/virologia
Doenças das Plantas/virologia
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
RNA Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Eukaryotic Initiation Factor-4E); 0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181998



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