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[PMID]:28692262
[Au] Autor:Mao GL; Yan Y; Chen Y; Wang BF; Xu FF; Zhang ZX; Lin F; Xu HH
[Ad] Endereço:State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University , Guangzhou, Guangdong 510642, People's Republic of China.
[Ti] Título:Family of Ricinus communis Monosaccharide Transporters and RcSTP1 in Promoting the Uptake of a Glucose-Fipronil Conjugate.
[So] Source:J Agric Food Chem;65(30):6169-6178, 2017 Aug 02.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enhancing the systemic distribution of a bioactive compound by exploiting the vascular transport system of a plant presents a means of reducing both the volume and frequency of pesticide/fungicide application. The foliar uptake of the glucose-fipronil conjugate N-[3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl)sulfinyl]-1H-pyrazol-5-yl]-1-(ß-d-glucopyranosyl)-1H-1,2,3-triazole-4-methanamine (GTF) achieved in castor bean (Ricinus communis) and its transport via the phloem are known to be mediated by monosaccharide transporter(s) [MST(s)], although neither the identity of the key MST(s) involved nor the mechanistic basis of its movement have yet to be described. On the basis of homology with Arabidopsis thaliana sugar transporters, the castor bean genome was concluded to harbor 53 genes encoding a sugar transporter, falling into the eight previously defined subfamilies INT, PMT, VGT, STP, ERD6, pGlucT, TMT, and SUT. Transcriptional profiling identified the product of RcSTP1 as a candidate for mediating GTF uptake, because this gene was induced by exposure of the plant to GTF. When RcSTP1 was transiently expressed in onion epidermis cells, the site of RcSTP1 deposition was shown to be the plasma membrane. A functional analysis based on RcSTP1 expression in Xenopus laevis oocytes demonstrated that its product has a high affinity for GTF. The long-distance root-to-shoot transport of GTF was enhanced in a transgenic soybean chimera constitutively expressing RcSTP1.
[Mh] Termos MeSH primário: Glucose/metabolismo
Inseticidas/metabolismo
Proteínas de Transporte de Monossacarídeos/metabolismo
Monossacarídeos/metabolismo
Proteínas de Plantas/metabolismo
Pirazóis/metabolismo
Ricinus/metabolismo
[Mh] Termos MeSH secundário: Transporte Biológico
Proteínas de Transporte de Monossacarídeos/genética
Floema/metabolismo
Proteínas de Plantas/genética
Ricinus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Monosaccharide Transport Proteins); 0 (Monosaccharides); 0 (Plant Proteins); 0 (Pyrazoles); IY9XDZ35W2 (Glucose); QGH063955F (fipronil)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02044


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[PMID]:28678952
[Au] Autor:Badaró MM; Salles MM; Leite VMF; Arruda CNF; Oliveira VC; Nascimento CD; Souza RF; Paranhos HFO; Silva-Lovato CH
[Ad] Endereço:Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Materiais Dentários e Prótese, Ribeirão Preto, SP, Brazil.
[Ti] Título:Clinical trial for evaluation of Ricinus communis and sodium hypochlorite as denture cleanser.
[So] Source:J Appl Oral Sci;25(3):324-334, 2017 May-Jun.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Objective: This study evaluated Ricinus communis and sodium hypochlorite solutions in terms of biofilm removal ability, remission of candidiasis, antimicrobial activity, and participant satisfaction. Material and Methods: It was conducted a controlled clinical trial, randomized, double-blind, and crossover. Sixty-four denture wearers with (n=24) and without candidiasis (n=40) were instructed to brush (3 times/day) and immerse their dentures (20 min/day) in different storage solutions (S1 / S2: 0.25% / 0.5% sodium hypochlorite; S3: 10% R. communis; S4: Saline).The trial period for each solution was seven days and a washout period of seven days was used before starting the use of another solution. The variables were analyzed at baseline and after each trial period. The biofilm of inner surfaces of maxillary dentures was disclosed, photographed, and total and dyed areas were measured (Image Tool software). The percentage of biofilm was calculated. Remission of candidiasis was assessed by visual scale and score were attributed. Antimicrobial activity was assessed by the DNA-Checkerboard hybridization method. Patient satisfaction was measured using a questionnaire. Results: S1 (4.41±7.98%) and S2 (2.93±5.23%) were more effective then S3 (6.95±10.93%) in biofilm remotion(P<0.0001). All solutions were different from the control (11.07±11.99%). S3 was the most effective solution in remission of candidiasis (50%), followed by S1 (46%). Concerning antimicrobial action, S1/S2 were similar and resulted in the lowest microorganism mean count (P=0.04), followed by S3. No significant differences were found with patient's satisfaction. Conclusions: 10% R. communis and 0.25% sodium hypochlorite were effective in biofilm removal, causing remission of candidiasis and reducing the formation of microbial colonies in denture surfaces. All solutions were approved by patients.
[Mh] Termos MeSH primário: Anti-Infecciosos Locais
Biofilmes/efeitos dos fármacos
Candidíase Bucal/tratamento farmacológico
Higienizadores de Dentadura
Prótese Total Superior/microbiologia
Ricinus/química
Hipoclorito de Sódio
[Mh] Termos MeSH secundário: Idoso
Análise de Variância
Contagem de Colônia Microbiana
Método Duplo-Cego
Feminino
Seres Humanos
Modelos Logísticos
Masculino
Satisfação do Paciente
Reprodutibilidade dos Testes
Propriedades de Superfície
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Anti-Infective Agents, Local); 0 (Denture Cleansers); DY38VHM5OD (Sodium Hypochlorite)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170706
[St] Status:MEDLINE


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[PMID]:28407195
[Au] Autor:Simões KS; Silva SA; Machado EL; Brasileiro HS
[Ad] Endereço:Núcleo de Melhoramento Genético e Biotecnologia, Centro de Ciências Agrárias Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brasil karinesimoes01@hotmail.com.
[Ti] Título:Development of TRAP primers for Ricinus communis L.
[So] Source:Genet Mol Res;16(2), 2017 Apr 13.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The objective of this article was to develop TRAP (target region amplification polymorphism) primers for castor bean, with the goal of making functional markers available for genetic studies about the species. To do this, oligonucleotides were designed based on ESTs, obtained from the NCBI (National Center for Biotechnology Information) databank, which code enzymes involved in metabolic routes of fatty acid synthesis, ricin synthesis, and resistance to castor bean pathogens. The forward primers were designed with the help of the Primer3 software and, for the reverse, six arbitrary primers were used. To standardize the amplification reactions, the following criteria were used to select the primers: sizes between 18 and 20 bp, guanine/cytosine (GC) in the range of 40 to 60%, and average annealing temperature between 55° and 62°C. The design quality of the primers was verified using the Net Primer application. Fifty-six primers were designed, which had an average GC percentage of 53.2%. A total of 336 combinations were obtained using the 56 fixed and 6 arbitrary primers. Based on polymerase chain reaction, 330 combinations (89%) presented good amplification patterns for the genomic DNA of castor bean. The size of the fragments amplified varied between 50 and 2072 bp. The TRAP primers designed and validated in this study are the first for castor bean and represent a significant increase in the molecular markers for this species.
[Mh] Termos MeSH primário: Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/métodos
Primers do DNA/genética
Etiquetas de Sequências Expressas
Polimorfismo Genético
Ricinus/genética
[Mh] Termos MeSH secundário: Pareamento de Bases
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029647


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[PMID]:28152474
[Au] Autor:Babu PR; Bhuvaneswar C; Sandeep G; Ramaiah CV; Rajendra W
[Ad] Endereço:Division of Molecular Biology, Department of Zoology, Sri Venkateswara University, Tirupati 517502, A.P, India.
[Ti] Título:Hepatoprotective role of Ricinus communis leaf extract against d-galactosamine induced acute hepatitis in albino rats.
[So] Source:Biomed Pharmacother;88:658-666, 2017 Apr.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Ricinus communis (RC) is a traditional medicinal plant which has been used by Chenchu and Yerukula tribes for treating their liver ailments. The present work is aimed to explore the hepatoprotective efficacy of Ricinus communis against d-galactosamine (D-GalN) induced hepatitis rat model and its therapeutic potential compared with standard drug, silymarin (100mg/kg.bw). In vitro antioxidant activity of Methanolic extract of Ricinus communis leaves (MERCL) was assayed through DPPH and H O free radical scavenging activity. Qualitative and quantitative analysis of MERCL using HPLC, demonstrated that Rutin was found to be predominant bioactive compound in the extract. Hepatitis was induced by treating the rats with D-GalN at a single intraperitoneal dose of 800mg/kg.bw. Serum markers viz, Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP) and Malondialdehyde (MDA) levels were significantly increased and the activity levels of antioxidant enzymes such as Superoxide dismutase (SOD),Catalase (CAT), Glutathione reductase (GR), Glutathione peroxidase (GPx), non-enzymatic antioxidant Glutathione (GSH) levels were decreased in the liver of hepatitis induced rats when compared to controls. Pre and post treatment with MERCL significantly altered the enzyme activities, GSH and MDA to normal levels. Histopathological observations also showed protective and curative effects of MERCL against D-GalN intoxication. These results demonstrated that MERCL significantly protected the liver from d-galactosamine induced hepatitis, improved the curative effect in the liver and hence, MERCL can be used as a potent hepatoprotective drug in future.
[Mh] Termos MeSH primário: Hepatite/tratamento farmacológico
Fígado/patologia
Extratos Vegetais/uso terapêutico
Folhas de Planta/química
Substâncias Protetoras/uso terapêutico
Ricinus/química
[Mh] Termos MeSH secundário: Doença Aguda
Animais
Antioxidantes/metabolismo
Biomarcadores/sangue
Compostos de Bifenilo/química
Cromatografia Líquida de Alta Pressão
Depuradores de Radicais Livres/farmacologia
Galactosamina
Glutationa/metabolismo
Hepatite/sangue
Hepatite/patologia
Peroxidação de Lipídeos/efeitos dos fármacos
Fígado/efeitos dos fármacos
Fígado/enzimologia
Malondialdeído/metabolismo
Metanol/química
Fitoterapia
Picratos/química
Substâncias Protetoras/farmacologia
Ratos Wistar
Rutina/análise
Silimarina/farmacologia
Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biomarkers); 0 (Biphenyl Compounds); 0 (Free Radical Scavengers); 0 (Picrates); 0 (Plant Extracts); 0 (Protective Agents); 0 (Silymarin); 0 (Thiobarbituric Acid Reactive Substances); 4Y8F71G49Q (Malondialdehyde); 5G06TVY3R7 (Rutin); 7535-00-4 (Galactosamine); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); GAN16C9B8O (Glutathione); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE


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[PMID]:28094539
[Au] Autor:Wang ML; Dzievit M; Chen Z; Morris JB; Norris JE; Barkley NA; Tonnis B; Pederson GA; Yu J
[Ad] Endereço:a USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA.
[Ti] Título:Genetic diversity and population structure of castor (Ricinus communis L.) germplasm within the US collection assessed with EST-SSR markers.
[So] Source:Genome;60(3):193-200, 2017 Mar.
[Is] ISSN:1480-3321
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Castor is an important oilseed crop and although its oil is inedible, it has multiple industrial and pharmaceutical applications. The entire US castor germplasm collection was previously screened for oil content and fatty acid composition, but its genetic diversity and population structure has not been determined. Based on the screening results of oil content, fatty acid composition, and country origins, 574 accessions were selected and genotyped with 22 polymorphic EST-SSR markers. The results from cluster analysis, population structure, and principal component analysis were consistent, and partitioned accessions into four subpopulations. Although there were certain levels of admixtures among groups, these clusters and subpopulations aligned with geographic origins. Both divergent and redundant accessions were identified in this study. The US castor germplasm collection encompasses a moderately high level of genetic diversity (pairwise dissimilarity coefficient = 0.53). The results obtained here will be useful for choosing accessions as parents to make crosses in breeding programs and prioritizing accessions for regeneration to improve germplasm management. A subset of 230 accessions was selected and will be planted in the field for establishing a core collection of the US castor germplasm. Further evaluation of the US castor germplasm collection is also discussed.
[Mh] Termos MeSH primário: Variação Genética
Genética Populacional
Ricinus/genética
[Mh] Termos MeSH secundário: Alelos
Análise por Conglomerados
Etiquetas de Sequências Expressas
Ácidos Graxos/química
Marcadores Genéticos
Genótipo
Geografia
Repetições de Microssatélites
Polimorfismo Genético
Análise de Componente Principal
Ricina/genética
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Genetic Markers); 9009-86-3 (Ricin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170320
[Lr] Data última revisão:
170320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170118
[St] Status:MEDLINE
[do] DOI:10.1139/gen-2016-0116


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[PMID]:27220628
[Au] Autor:Jaison S; Muthukumar T
[Ad] Endereço:Root and Soil Biology Laboratory, Department of Botany, Bharathiar University, Coimbatore, 641046, Tamilnadu, India.
[Ti] Título:Chromium Accumulation in Medicinal Plants Growing Naturally on Tannery Contaminated and Non-contaminated Soils.
[So] Source:Biol Trace Elem Res;175(1):223-235, 2017 Jan.
[Is] ISSN:1559-0720
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Herbal preparations used to treat human ailments globally can be contaminated with various heavy metals (HMs) originating from the raw materials or from the manufacturing processes. Therefore, we assessed 22 medicinal plants growing naturally on tannery pollutant contaminated (Site-C) and non-contaminated (Site-NC) sites for their ability to accumulate chromium (Cr). The Cr contents in soil and various plant parts were estimated using an atomic absorption spectrophotometer. Translocation and bioconcentration factors were calculated. The soil at Site-C had 27-fold higher concentration of total Cr than at Site-NC. Chromium accumulation is reported for the first time in 50 % of the medicinal plants examined and varied significantly among the sites. Shoots of Ricinus communis and Amaranthus viridis had maximum concentrations of Cr at Site-C, whereas in Site-NC, none of the plants had Cr accumulation >30 ppm. Ricinus communis, Amaranthus viridis, and Amaranthus spinosus had translocation factor (TF) greater than the one in the Site-C and Lantana camara had TF >1 in Site-NC. The bioconcentration factor (BCF) was >1 only for Ricinus communis at both the sites. The majority of the medicinal plants at Site-NC had Cr content exceeding the permissible limit of 2 ppm suggested for herbal raw material. The results of the study clearly emphasize the need for screening plants of therapeutic value for the presence of HMs even when collected from non-contaminated soils. Moreover, proportional allocation of Cr in different plant parts provided an insight on the safety of these parts when specifically used in herbal preparations.
[Mh] Termos MeSH primário: Amaranthus/metabolismo
Cromo/metabolismo
Brotos de Planta/metabolismo
Plantas Medicinais/metabolismo
Ricinus/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 0R0008Q3JB (Chromium)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160526
[St] Status:MEDLINE
[do] DOI:10.1007/s12011-016-0740-1


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[PMID]:27457556
[Au] Autor:Hadi F; Ali N; Fuller MP
[Ad] Endereço:Department of Biotechnology, University of Malakand, Chakdara, Dir Lower, Khyber Pakhtunkhwa, 18800, Pakistan. dr.fhadi@uom.edu.pk.
[Ti] Título:Molybdenum (Mo) increases endogenous phenolics, proline and photosynthetic pigments and the phytoremediation potential of the industrially important plant Ricinus communis L. for removal of cadmium from contaminated soil.
[So] Source:Environ Sci Pollut Res Int;23(20):20408-20430, 2016 Oct.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Cadmium (Cd) in agricultural soil negatively affects crops yield and compromises food safety. Remediation of polluted soil is necessary for the re-establishment of sustainable agriculture and to prevent hazards to human health and environmental pollution. Phytoremediation is a promising technology for decontamination of polluted soil. The present study investigated the effect of molybdenum (Mo) (0.5, 1.0 and 2.0 ppm) on endogenous production of total phenolics and free proline, plant biomass and photosynthetic pigments in Ricinus communis plants grown in Cd (25, 50 and 100 ppm) contaminated soils and the potential for Cd phytoextraction. Mo was applied via seed soaking, soil addition and foliar spray. Foliar sprays significantly increased plant biomass, Cd accumulation and bioconcentration. Phenolic concentrations showed significantly positive correlations with Cd accumulation in roots (R  = 0.793, 0.807 and 0.739) and leaves (R  = 0.707, 721 and 0.866). Similarly, proline was significantly positively correlated with Cd accumulation in roots (R  = 0.668, 0.694 and 0.673) and leaves (R  = 0.831, 0.964 and 0.930). Foliar application was found to be the most effective way to deliver Mo in terms of increase in plant growth, Cd accumulation and production of phenolics and proline.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Produtos Agrícolas
Molibdênio
Ricinus
Poluentes do Solo
[Mh] Termos MeSH secundário: Cádmio/análise
Cádmio/metabolismo
Produtos Agrícolas/química
Produtos Agrícolas/efeitos dos fármacos
Produtos Agrícolas/metabolismo
Molibdênio/análise
Molibdênio/metabolismo
Molibdênio/farmacologia
Fenóis/análise
Fenóis/metabolismo
Folhas de Planta/química
Folhas de Planta/metabolismo
Raízes de Plantas/química
Raízes de Plantas/metabolismo
Prolina/análise
Prolina/metabolismo
Ricinus/química
Ricinus/efeitos dos fármacos
Ricinus/metabolismo
Sementes/metabolismo
Poluentes do Solo/análise
Poluentes do Solo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenols); 0 (Soil Pollutants); 00BH33GNGH (Cadmium); 81AH48963U (Molybdenum); 9DLQ4CIU6V (Proline)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160727
[St] Status:MEDLINE


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[PMID]:27440939
[Au] Autor:Peuke AD
[Ad] Endereço:ADP International Plant Science Consulting, Talstraße 8, D-79194 Gundelfingen, Germany AD_Peuke@web.de.
[Ti] Título:ABA flow modelling in Ricinus communis exposed to salt stress and variable nutrition.
[So] Source:J Exp Bot;67(18):5301-5311, 2016 Oct.
[Is] ISSN:1460-2431
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In a series of experiments with Ricinus communis, abscisic acid (ABA) concentrations in tissues and transport saps, its de novo biosynthesis, long-distance transport, and metabolism (degradation) were affected by nutritional conditions, nitrogen (N) source, and nutrient limitation, or salt stress. In the present study these data were statistically re-evaluated, and new correlations presented that underpin the importance of this universal phytohormone. The biggest differences in ABA concentration were observed in xylem sap. N source had the strongest effect; however, nutrient limitation (particularly phosphorus limitation) and salt also had significant effects. ABA was found in greater concentration in phloem sap compared with xylem sap; however, the effect of treatment on ABA concentration in phloem was lower. In the leaves, ABA concentration was most variable compared with the other tissues. This variation was only affected by the N source. In roots, ABA was significantly decreased by nutrient limitation. Of the compartments in which ABA was quantified, xylem sap ABA concentration was most significantly correlated with leaf stomatal conductance and leaf growth. Additionally, ABA concentration in xylem was significantly correlated to that in phloem, indicating a 6-fold concentration increase from xylem to phloem. The ABA flow model showed that biosynthesis of ABA in roots affected the xylem flow of ABA. Moreover, ABA concentration in xylem affected the degradation of the phytohormone in shoots and also its export from shoots via phloem. The role of phloem transport is discussed since it stimulates ABA metabolism in roots.
[Mh] Termos MeSH primário: Ácido Abscísico/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Ricinus/metabolismo
Tolerância a Sal/fisiologia
[Mh] Termos MeSH secundário: Ácido Abscísico/fisiologia
Floema/metabolismo
Reguladores de Crescimento de Planta/fisiologia
Fenômenos Fisiológicos Vegetais
Raízes de Plantas/metabolismo
Ricinus/fisiologia
Estresse Fisiológico/fisiologia
Xilema/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Growth Regulators); 72S9A8J5GW (Abscisic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160722
[St] Status:MEDLINE


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[PMID]:27414256
[Au] Autor:Zhang H; Guo Q; Yang J; Ma J; Chen G; Chen T; Zhu G; Wang J; Zhang G; Wang X; Shao C
[Ad] Endereço:Shenyang Academy of Environmental Sciences, China; Center for Environmental Remediation, Institute of Geographical Sciences and Natural Resources, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: zhanghanzhihan@163.com.
[Ti] Título:Comparison of chelates for enhancing Ricinus communis L. phytoremediation of Cd and Pb contaminated soil.
[So] Source:Ecotoxicol Environ Saf;133:57-62, 2016 Nov.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We studied chelate effects on castor bean (Ricinus communis L.) growth. These effects included Cd and Pb accumulation in plant tissues and the chemical behavior of Cd and Pb in the plant rhizosphere and non-rhizosphere. Tests were conducted in a glasshouse using the rhizobag method. Two castor bean cultivars (Zibo-3 and Zibo-9) were grown in soil contaminated with 3.53mg/kg Cd and 274mg/kg Pb. The soil was treated with citric acid (CA), ethylenediamine disuccinic acid (EDDS) or ethylenediamine tetraacetic acid (EDTA) (5mmol/kg). EDDS-treated soil produced 28.8% and 59.4% greater biomass for Zibo-3 and Zibo-9 respectively. In contrast, CA and EDTA inhibited the growth of the two cultivars. Zibo-9 had greater tolerance than Zibo-3 to chelate toxicity. Based on Cd and Pb plant uptake, EDDS could substitute for EDTA for phytoremediation of Cd in soil. EDTA was the most effective of the three chelates for Pb phytoremediation but it is less suitable for field use due to toxicology environmental persistence. Acid extractable Cd and Pb in the rhizosphere or reducible Cd and Pb in the non-rhizosphere of soil were the main influences on Cd and Pb accumulation in castor bean.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Cádmio/toxicidade
Quelantes/farmacologia
Chumbo/toxicidade
Ricinus/efeitos dos fármacos
Poluentes do Solo/toxicidade
[Mh] Termos MeSH secundário: Biomassa
Cádmio/análise
Semente de Rícino/química
Semente de Rícino/efeitos dos fármacos
Ácido Cítrico/farmacologia
Ácido Edético/farmacologia
Etilenodiaminas/farmacologia
Chumbo/análise
Raízes de Plantas/química
Caules de Planta/química
Rizosfera
Ricinus/química
Poluentes do Solo/análise
Succinatos/farmacologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 ((S,S)-ethylenediaminedisuccinate); 0 (Chelating Agents); 0 (Ethylenediamines); 0 (Soil Pollutants); 0 (Succinates); 00BH33GNGH (Cadmium); 2968PHW8QP (Citric Acid); 2P299V784P (Lead); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170123
[Lr] Data última revisão:
170123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160715
[St] Status:MEDLINE


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[PMID]:27372755
[Au] Autor:Bergamin E; Couture JF
[Ad] Endereço:Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON, Canada.
[Ti] Título:Preparation, Biochemical Analysis, and Structure Determination of SET Domain Histone Methyltransferases.
[So] Source:Methods Enzymol;573:209-40, 2016.
[Is] ISSN:1557-7988
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In eukaryotes, several lysine residues on histone proteins are methylated. This posttranslational modification is linked to a myriad of nuclear-based transactions such as epigenetic inheritance of heterochromatin, regulation of gene expression, DNA damage repair, and DNA replication. The majority of the enzymes responsible for writing these marks onto chromatin belong to the SET domain family of histone lysine methyltransferases. Although they often share important structural features, including a conserved catalytic domain, SET domain enzymes use different mechanisms to achieve substrate recognition, mono-, di-, or trimethylate lysine residues and some require other proteins to achieve maximal methyltransferase activity. In this chapter, we summarize our efforts to purify, crystallize, and enzymatically characterize SET domain enzymes with a specific focus on the histone H3K27 monomethyltransferase ATXR5.
[Mh] Termos MeSH primário: Histona-Lisina N-Metiltransferase/química
Histona-Lisina N-Metiltransferase/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Cristalografia por Raios X/métodos
Ensaios Enzimáticos/métodos
Escherichia coli/genética
Histona-Lisina N-Metiltransferase/genética
Histona-Lisina N-Metiltransferase/isolamento & purificação
Seres Humanos
Modelos Moleculares
Proteínas de Plantas/química
Proteínas de Plantas/genética
Proteínas de Plantas/isolamento & purificação
Proteínas de Plantas/metabolismo
Domínios Proteicos
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Ricinus/química
Ricinus/genética
Ricinus/metabolismo
Alinhamento de Sequência
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Recombinant Proteins); EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160704
[St] Status:MEDLINE



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