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  1 / 4199 MEDLINE  
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[PMID]:29207242
[Au] Autor:Sobolev V; Arias R; Goodman K; Walk T; Orner V; Faustinelli P; Massa A
[Ad] Endereço:National Peanut Research Laboratory, Agricultural Research Service, United States Department of Agriculture , P.O. Box 509, Dawson, Georgia 39842, United States.
[Ti] Título:Suppression of Aflatoxin Production in Aspergillus Species by Selected Peanut (Arachis hypogaea) Stilbenoids.
[So] Source:J Agric Food Chem;66(1):118-126, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aspergillus flavus is a soil fungus that commonly invades peanut seeds and often produces carcinogenic aflatoxins. Under favorable conditions, the fungus-challenged peanut plant produces and accumulates resveratrol and its prenylated derivatives in response to such an invasion. These prenylated stilbenoids are considered peanut antifungal phytoalexins. However, the mechanism of peanut-fungus interaction has not been sufficiently studied. We used pure peanut stilbenoids arachidin-1, arachidin-3, and chiricanine A to study their effects on the viability of and metabolite production by several important toxigenic Aspergillus species. Significant reduction or virtually complete suppression of aflatoxin production was revealed in feeding experiments in A. flavus, Aspergillus parasiticus, and Aspergillus nomius. Changes in morphology, spore germination, and growth rate were observed in A. flavus exposed to the selected peanut stilbenoids. Elucidation of the mechanism of aflatoxin suppression by peanut stilbenoids could provide strategies for preventing plant invasion by the fungi that produce aflatoxins.
[Mh] Termos MeSH primário: Aflatoxinas/metabolismo
Arachis/química
Aspergillus/efeitos dos fármacos
Aspergillus/metabolismo
Estilbenos/farmacologia
[Mh] Termos MeSH secundário: Aspergillus/crescimento & desenvolvimento
Hemiterpenos/farmacologia
Esterigmatocistina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aflatoxins); 0 (Hemiterpenes); 0 (Stilbenes); 0 (arachidin-1); 0 (arachidin-3); 0 (chiricanine A); 10048-13-2 (Sterigmatocystin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04542


  2 / 4199 MEDLINE  
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[PMID]:29244878
[Au] Autor:Chi X; Zhang Z; Chen N; Zhang X; Wang M; Chen M; Wang T; Pan L; Chen J; Yang Z; Guan X; Yu S
[Ad] Endereço:Shandong Peanut Research Institute, Qingdao, Shandong, P. R. China.
[Ti] Título:Isolation and functional analysis of fatty acid desaturase genes from peanut (Arachis hypogaea L.).
[So] Source:PLoS One;12(12):e0189759, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Fatty acid desaturases are enzymes that introduce double bonds into fatty acyl chains. Extensive studies of fatty acid desaturases have been done in many plants. However, less is known about the diversity of this gene family in peanut (Arachis hypogaea L.), an important oilseed crop that is cultivated worldwide. RESULTS: In this study, twelve novel AhFADs genes were identified and isolated from peanut. Quantitative real-time PCR analysis indicated that the transcript abundances of AhFAB2-2 and AhFAD3-1 were higher in seeds than in other tissues examined, whereas the AhADS and AhFAD7-1 transcripts were more abundant in leaves. AhFAB2-3, AhFAD3-2, AhFAD4, AhSLD-4, and AhDES genes were highly expressed in flowers, whereas AhFAD7-2, AhSLD-2, and AhSLD-3 were expressed most strongly in stems. During seed development, the expressions of AhFAB2-2, AhFAD3-1, AhFAD7-1, and AhSLD-3 gradually increased in abundance, reached a maximum expression level, and then decreased. The AhFAB2-3, AhFAD3-2, AhFAD4, AhADS, and AhDES transcript levels remained relatively high at the initial stage of seed development, but decreased thereafter. The AhSLD-4 transcript level remained relatively low at the initial stage of seed development, but showed a dramatic increase in abundance at the final stage. The AhFAD7-2 and AhSLD-2 transcript levels remained relatively high at the initial stage of seed development, but then decreased, and finally increased again. The AhFAD transcripts were differentially expressed following exposure to abiotic stresses or abscisic acid. Moreover, the functions of one AhFAD6 and four AhSLD genes were confirmed by heterologous expression in Synechococcus elongates or Saccharomyces cerevisiae. CONCLUSIONS: The present study provides valuable information that improves understanding of the biological roles of FAD genes in fatty acid synthesis, and will help peanut breeders improve the quality of peanut oil via molecular design breeding.
[Mh] Termos MeSH primário: Arachis/enzimologia
Ácidos Graxos Dessaturases/genética
Ácidos Graxos Dessaturases/isolamento & purificação
[Mh] Termos MeSH secundário: Clonagem Molecular
Ácidos Graxos Dessaturases/química
Flores/enzimologia
Regulação da Expressão Gênica de Plantas/genética
Família Multigênica/genética
Filogenia
Folhas de Planta/enzimologia
Sementes/enzimologia
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.14.19.- (Fatty Acid Desaturases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189759


  3 / 4199 MEDLINE  
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[PMID]:28456621
[Au] Autor:Johansson EK; Bergström A; Kull I; Lind T; Söderhäll C; van Hage M; Wickman M; Ballardini N; Wahlgren CF
[Ad] Endereço:Dermatology and Venereology Unit, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden; Dermatological and Venereal Clinic, Södersjukhuset, Stockholm, Sweden. Electronic address: emma.k.johansson@sll.se.
[Ti] Título:IgE sensitization in relation to preschool eczema and filaggrin mutation.
[So] Source:J Allergy Clin Immunol;140(6):1572-1579.e5, 2017 Dec.
[Is] ISSN:1097-6825
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Eczema (atopic dermatitis) is associated with an increased risk of having IgE antibodies. IgE sensitization can occur through an impaired skin barrier. Filaggrin gene (FLG) mutation is associated with eczema and possibly also with IgE sensitization. OBJECTIVE: We sought to explore the longitudinal relation between preschool eczema (PSE), FLG mutation, or both and IgE sensitization in childhood. METHODS: A total of 3201 children from the BAMSE (Children Allergy Milieu Stockholm Epidemiology) birth cohort recruited from the general population were included. Regular parental questionnaires identified children with eczema. Blood samples were collected at 4, 8, and 16 years of age for analysis of specific IgE. FLG mutation analysis was performed on 1890 of the children. RESULTS: PSE was associated with IgE sensitization to both food allergens and aeroallergens up to age 16 years (overall adjusted odds ratio, 2.30; 95% CI, 2.00-2.66). This association was even stronger among children with persistent PSE. FLG mutation was associated with IgE sensitization to peanut at age 4 years (adjusted odds ratio, 1.88; 95% CI, 1.03-3.44) but not to other allergens up to age 16 years. FLG mutation and PSE were not effect modifiers for the association between IgE sensitization and PSE or FLG mutation, respectively. Sensitized children with PSE were characterized by means of polysensitization, but no other specific IgE sensitization patterns were found. CONCLUSIONS: PSE is associated with IgE sensitization to both food allergens and aeroallergens up to 16 years of age. FLG mutation is associated with IgE sensitization to peanut but not to other allergens. Sensitized children with preceding PSE are more often polysensitized.
[Mh] Termos MeSH primário: Eczema/imunologia
Hipersensibilidade Alimentar/imunologia
Proteínas de Filamentos Intermediários/genética
Mutação/genética
Pele/imunologia
[Mh] Termos MeSH secundário: Adolescente
Alérgenos/imunologia
Arachis/imunologia
Criança
Pré-Escolar
Estudos de Coortes
Análise Mutacional de DNA
Eczema/epidemiologia
Eczema/genética
Feminino
Hipersensibilidade Alimentar/epidemiologia
Hipersensibilidade Alimentar/genética
Estudos de Associação Genética
Genótipo
Seres Humanos
Imunização
Imunoglobulina E/metabolismo
Masculino
Pele/patologia
Suécia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Intermediate Filament Proteins); 0 (filaggrin); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


  4 / 4199 MEDLINE  
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[PMID]:28873607
[Au] Autor:Limmongkon A; Nopprang P; Chaikeandee P; Somboon T; Wongshaya P; Pilaisangsuree V
[Ad] Endereço:Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand. Electronic address: apinunl@nu.ac.th.
[Ti] Título:LC-MS/MS profiles and interrelationships between the anti-inflammatory activity, total phenolic content and antioxidant potential of Kalasin 2 cultivar peanut sprout crude extract.
[So] Source:Food Chem;239:569-578, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Peanut is a potent natural source of phytochemical compounds and is associated with human health. In the present study, we determined the biological activity and chemical constituents of peanuts germinated for 0-9days. The ungerminated seed coat exhibited the highest antioxidant potential, phenolic compound content and anti-inflammatory activity. During the germination process, on the first germination day, root extract showed the highest antioxidant potential, phenolic compound content and anti-inflammatory activity. The kernel exhibited a low phenolic compound content and antioxidant activity at the early stage of germination and significantly increased after 9days of germination. Resveratrol increased to 7.19±0.07µg/g dry weight on the second day of germination. LC-MS/MS showed a variety of phenolic compounds and stilbene derivatives in different parts of germinated peanut. These results suggest that the peanut sprout exerts high anti-inflammatory effects that may be related to the polyphenolic content and antioxidant properties.
[Mh] Termos MeSH primário: Arachis
[Mh] Termos MeSH secundário: Antioxidantes
Germinação
Seres Humanos
Fenóis
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Phenols)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  5 / 4199 MEDLINE  
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[PMID]:29040293
[Au] Autor:Hake AA; Shirasawa K; Yadawad A; Sukruth M; Patil M; Nayak SN; Lingaraju S; Patil PV; Nadaf HL; Gowda MVC; Bhat RS
[Ad] Endereço:Department of Biotechnology, University of Agricultural Sciences, Dharwad, India.
[Ti] Título:Mapping of important taxonomic and productivity traits using genic and non-genic transposable element markers in peanut (Arachis hypogaea L.).
[So] Source:PLoS One;12(10):e0186113, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A mapping population of recombinant inbred lines (RILs) derived from TMV 2 and its mutant, TMV 2-NLM was employed for mapping important taxonomic and productivity traits using genic and non-genic transposable element markers in peanut. Single nucleotide polymorphism and copy number variation using RAD-Sequencing data indicated very limited polymorphism between TMV 2 and TMV 2-NLM. But phenotypically they differed significantly for many taxonomic and productivity traits. Also, the RIL population showed significant variation for a few additional agronomic traits. A genetic linkage map of 1,205.66 cM was constructed using 91 genic and non-genic Arachis hypogaea transposable element (AhTE) markers. Using single marker analysis and QTL analysis, the markers with high phenotypic variance explained (PVE) were identified for branching pattern (32.3%), number of primary and secondary branches (19.9% and 28.4%, respectively), protein content (26.4%), days to 50% flowering (22.0%), content of oleic acid (15.1%), test weight (13.6%) and pod width (12.0%). Three genic markers (AhTE0357, AhTE0391, AhTE0025) with Arachis hypogaea miniature inverted-repeat transposable element (AhMITE1) activity in the genes Araip.TG1BL (B02 chromosome), Aradu.7N61X (A09 chromosome) and Aradu.7065G (A07 chromosome), respectively showed strong linkage with these taxonomic, productivity and quality traits. Since TMV 2 and TMV 2-NLM differed subtly at DNA level, the background noise in detecting the marker-trait associations was minimum; therefore, the markers identified in this study for the taxonomic and productivity traits may be significant and useful in peanut molecular breeding.
[Mh] Termos MeSH primário: Arachis/genética
Elementos de DNA Transponíveis/genética
Marcadores Genéticos/genética
Locos de Características Quantitativas/genética
[Mh] Termos MeSH secundário: Arachis/crescimento & desenvolvimento
Cruzamento
Mapeamento Cromossômico
Classificação
Ligação Genética
Genoma de Planta
Fenótipo
Polimorfismo de Nucleotídeo Único/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186113


  6 / 4199 MEDLINE  
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[PMID]:28981520
[Au] Autor:Chen C; Xia S; Deng R; Liu C; Shi G
[Ad] Endereço:College of Life Sciences, Huaibei Normal University, Huaibei, P. R., China.
[Ti] Título:AhIRT1 and AhNRAMP1 metal transporter expression correlates with Cd uptake in peanuts under iron deficiency.
[So] Source:PLoS One;12(10):e0185144, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fe deficiency may increase Cd accumulation in peanuts. However, the mechanisms are not yet fully understood. In the present study, two contrasting peanut cultivars, Luhua 8 (low seed-Cd cultivar) and Zhenghong 3 (high seed-Cd cultivar) were used to investigate the effect of Fe deficiency on the uptake and accumulation of cadmium (Cd) by hydroponic experiments. Under Fe-sufficient conditions, compared with Luhua 8, Zhenghong 3 had higher specific root length (SRL) and proportion of fine roots with a lower Km for Cd and showed slightly higher expression of AhIRT1 and AhNRAMP1 in the roots. These traits may be responsible for high capacity for Cd accumulation in Zhenghong 3. Under Fe deficiency, the increase of Cd accumulation was much larger in Zhenghong 3 than in Luhua 8. Kinetics studies revealed that the Vmax for Cd influx was 1.56-fold higher in Fe-deficient plants than in Fe-sufficient plants for Zhenghong 3, versus 0.48-fold higher for Luhua 8. Moreover, the increased expression levels of AhIRT1 and AhNRAMP1 induced by Fe deficiency was higher in Zhenghong 3 than in Luhua 8. Yeast complementation assays suggested that the AhIRT1 and AhNRAMP1 may function as transporters involved in Cd uptake. In conclusion, the different Cd accumulation between the two cultivars under Fe deficiency may be correlated with Vmax value for Cd uptake and the expression levels of AhIRT1 and AhNRAMP1 in the roots.
[Mh] Termos MeSH primário: Arachis/metabolismo
Cádmio/metabolismo
Ferro/deficiência
Proteínas de Membrana Transportadoras/metabolismo
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Arachis/crescimento & desenvolvimento
Clorofila/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Transport Proteins); 0 (Plant Proteins); 00BH33GNGH (Cadmium); 1406-65-1 (Chlorophyll); E1UOL152H7 (Iron)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185144


  7 / 4199 MEDLINE  
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[PMID]:28950267
[Au] Autor:Schocker F; Scharf A; Kull S; Jappe U
[Ad] Endereço:Division of Clinical and Molecular Allergology, Research Center Borstel, Priority Research Area Asthma and Allergy, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Borstel, Germany.
[Ti] Título:Detection of the Peanut Allergens Ara h 2 and Ara h 6 in Human Breast Milk: Development of 2 Sensitive and Specific Sandwich ELISA Assays.
[So] Source:Int Arch Allergy Immunol;174(1):17-25, 2017.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Little is known about breast milk as a vehicle for tolerance development or sensitization to peanuts very early in life. Thus, well-characterized and highly sensitive detection systems for the reliable determination of peanut allergens in breast milk are mandatory. METHODS: For the quantification of the marker allergens Ara h 2 and Ara h 6 in the low nanogram per milliliter range in breast milk samples of a German cohort, sensitive and highly specific sandwich ELISAs were optimized and validated. RESULTS: The Ara h 2 ELISA revealed a limit of detection (LOD) of 1.3 ng Ara h 2/mL and a quantification range of 2.3-250 ng/mL, the Ara h 6 ELISA showed an LOD of 0.7 ng/mL and a working range of 1.1-14.4 ng/mL. The assays showed no relevant cross-reactivity against other potentially cross-reactive legume, seed, and tree nut extracts (<0.01%, except for Ara h 1 in the Ara h 2 ELISA <0.1%). Ara h 2 was detectable in breast milk samples from 14/40 (35%) of the participants in concentrations from 2.3 to 184 ng/mL, Ara h 6 appeared in 9/40 (22.5%) of the lactating mothers between 1.1 and 9.7 ng/mL, and 1 highly positive sample with 79 ng/mL. Both allergens appeared at the same time points, but Ara h 6 in lower concentrations than Ara h 2. CONCLUSIONS: Sensitive and specific diagnostic tools for the determination of Ara h 2 and Ara h 6 in human breast milk were established. The kinetics of secreted Ara h 2 and Ara h 6 seem to be similar but with a difference in concentration. Follow-up investigations on their tolerogenic or sensitizing properties in breast milk become now accessible.
[Mh] Termos MeSH primário: Albuminas 2S de Plantas/análise
Antígenos de Plantas/análise
Ensaio de Imunoadsorção Enzimática/métodos
Glicoproteínas/análise
Leite Humano/química
Hipersensibilidade a Amendoim/diagnóstico
Proteínas de Plantas/imunologia
[Mh] Termos MeSH secundário: Alérgenos/análise
Arachis/imunologia
Reações Cruzadas/imunologia
Feminino
Seres Humanos
Lactação/fisiologia
Limite de Detecção
Hipersensibilidade a Amendoim/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2S Albumins, Plant); 0 (Allergens); 0 (Antigens, Plant); 0 (Ara h 2 allergen, Arachis hypogaea); 0 (Ara h 6 allergen, Arachis hypogaea); 0 (Glycoproteins); 0 (Plant Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1159/000479388


  8 / 4199 MEDLINE  
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[PMID]:28890356
[Au] Autor:Akuete K; Guffey D; Israelsen RB; Broyles JM; Higgins LJ; Green TD; Naimi DR; MacGinnitie AJ; Vitalpur G; Minard CG; Davis CM
[Ad] Endereço:Immunology, Allergy, and Rheumatology Section, Texas Children's Hospital, Houston, Texas; Department of Pediatrics, Baylor College of Medicine, Houston, Texas.
[Ti] Título:Multicenter prevalence of anaphylaxis in clinic-based oral food challenges.
[So] Source:Ann Allergy Asthma Immunol;119(4):339-348.e1, 2017 Oct.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Although previous single-center studies report the rate of anaphylaxis for oral food challenges (OFCs) as 9% to 11%, little is known regarding the epidemiology of clinical OFCs across multiple centers in the United States. OBJECTIVE: To examine the epidemiology, symptoms, and treatment of clinical low-risk OFCs in the nonresearch setting. METHODS: Data were obtained from 2008 to 2013 through a physician survey in 5 food allergy centers geographically distributed across the United States. Allergic reaction rates and the association of reaction rates with year, hospital, and demographics were determined using a linear mixed model. Meta-analysis was used to pool the proportion of reactions and anaphylaxis with inverse-variance weights using a random-effects model with exact confidence intervals (CIs). RESULTS: A total of 6,377 OFCs were performed, and the pooled estimate of anaphylaxis was 2% (95% CI, 1%-3%). The rate of allergic reactions was 14% (95% CI, 13%-16%) and was consistent during the study period (P = .40). Reaction rates ranged from 13% to 33%. Males reacted 16% more frequently than females (95% CI, 4%-37.5%; P = .04). Foods challenged in 2013 varied geographically, with peanut as the most challenged food in the Northeast, Midwest, and West and egg as the most challenged in the South. CONCLUSION: As the largest national survey of allergic reactions of clinical open OFCs in a nonresearch setting in the United States, this study found that performing clinical nonresearch open low-risk OFCs results in few allergic reactions, with 86% of challenges resulting in no reactions and 98% without anaphylaxis.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Anafilaxia/epidemiologia
Hipersensibilidade Alimentar/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Anafilaxia/diagnóstico
Anafilaxia/fisiopatologia
Arachis/química
Arachis/imunologia
Criança
Pré-Escolar
Hipersensibilidade Alimentar/diagnóstico
Hipersensibilidade Alimentar/fisiopatologia
Seres Humanos
Incidência
Lactente
Modelos Lineares
Prevalência
Risco
Fatores Sexuais
Testes Cutâneos
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Allergens)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE


  9 / 4199 MEDLINE  
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[PMID]:28890022
[Au] Autor:Scott-Taylor TH; Axinia SC; Strobel S
[Ad] Endereço:Faculty of Life Sciences and Computing, London Metropolitan University, London, United Kingdom. Electronic address: t.scott-taylor@londonmet.ac.uk.
[Ti] Título:Lymphoproliferative responses to dendritic cell presentation of sensitizing allergens in atopic children with multiple allergies.
[So] Source:Ann Allergy Asthma Immunol;119(3):274-283, 2017 Sep.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Peripheral blood mononuclear cells (PBMCs) proliferate inconsistently, rendering current lymphoproliferation assays unreliable in diagnosis. OBJECTIVE: To investigate the utility and nature of proliferation responses in allergy by comparison of the standard lymphoproliferation with a new dendritic cell (DC) stimulated assay. METHODS: Monocyte-derived DCs were pulsed with allergens and incubated with autologous T cells for 7 days. DC-stimulated and standard PBMC proliferation responses to 3 common dietary allergens in children with allergy and without atopy were measured by incorporation of tritiated thymidine and reduction of carboxyl fluorescein succinimidyl ester staining. RESULTS: The DC presentation of sensitizing allergens induced significantly higher proliferative responses than PBMC stimulation (P = .04) and greater distinction between normal and allergic responses. DC-induced stimulation indices of children without sensitivity and those with allergy were significantly different with all 3 foods (P < .001). All children with allergy presented with peanut allergy and 12 of 14 (86%) ß-lactoglobulin-pulsed DC preparations proliferated more than 3.3-fold above un-pulsed cells, but 8 of 18 children (44%) with ovalbumin egg allergy showed proliferation below this level. The stimulation index of DC tritiated thymidine incorporation correlated closely with carboxyl fluorescein succinimidyl ester reduction (P < .001). Sensitivity of detection of peanut, milk, or egg allergy was 100%, 85.7%, or 55.6% and specificity was 60%, 88.9%, or 86.7%, respectively. DC-stimulated T cells expressed increased levels of CD45 RO and CD25 and most produced interferon-γ. DC-stimulated proliferation correlated with total immunoglobulin E and peanut antigen-stimulated proliferation correlated with peanut specific immunoglobulin E (P = .03). CONCLUSION: The DC-induced lymphoproliferation had higher sensitivity, specificity, and reproducibility than the standard assay and caused increased memory and activated T-cell proliferation in children with food allergy.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Células Dendríticas/imunologia
Hipersensibilidade Imediata/imunologia
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Adolescente
Animais
Apresentação do Antígeno
Arachis/imunologia
Proliferação Celular
Criança
Pré-Escolar
Feminino
Seres Humanos
Hipersensibilidade Imediata/sangue
Imunoglobulina E/sangue
Imunoglobulina E/imunologia
Leucócitos Mononucleares/imunologia
Masculino
Leite/imunologia
Ovalbumina/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 37341-29-0 (Immunoglobulin E); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE


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[PMID]:28890021
[Au] Autor:Valcour A; Jones JE; Lidholm J; Borres MP; Hamilton RG
[Ad] Endereço:Laboratory Corporation of America, Burlington, North Carolina. Electronic address: valcoua@labcorp.com.
[Ti] Título:Sensitization profiles to peanut allergens across the United States.
[So] Source:Ann Allergy Asthma Immunol;119(3):262-266.e1, 2017 Sep.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Measurement of IgE antibody to peanut components can aid in the prediction of allergic responses the food. OBJECTIVE: To investigate the association between patient demographics (age, location) and allergic sensitization to peanut components across the United States. METHODS: Serum samples from 12,155 individuals with peanut extract specific IgE levels of 0.35 kUA/L or higher were analyzed for IgE antibodies to Ara h 1, 2, 3, 8, and 9 by ImmunoCAP. RESULTS: Among this population of peanut sensitized individuals, 79.1% of children (<3 years old) were sensitized to one or more peanut storage proteins (Ara h 1, 2, and/or 3), in contrast to 64.2% of adolescents (12-15 years old) and 22.1% of adults (>20 years old). Although sensitization was more prevalent to Ara h 2 than to the other storage proteins, a sizable fraction of patients were sensitized to Ara h 1 and/or 3 but not to Ara h 2 (eg, 13% of children <3 years old). Moreover, 9.6% of children, 10.2% of adolescents, and 10.5% of adults were sensitized to Ara h 9, whereas 2.4% of children, 49.4% of adolescents, and 42.9% of adults produced IgE to Ara h 8 (pathogenesis-related protein 10). Sensitization to Ara h 8 alone was markedly higher in the Northeastern United States relative to other regions of the country. CONCLUSION: We conclude that sensitization to individual peanut components is highly dependent on age and geographic location. Given that a severe allergic reaction to peanut is unlikely in individuals with isolated sensitization to Ara h 8, a sizable fraction of patients, in particular adolescents and adults, may be at lower risk than anticipated based only on demonstration of sensitization to whole peanut extract.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Antígenos de Plantas/imunologia
Arachis/imunologia
Hipersensibilidade a Amendoim/imunologia
Proteínas de Plantas/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Criança
Pré-Escolar
Seres Humanos
Imunoglobulina E/sangue
Imunoglobulina E/imunologia
Lactente
Recém-Nascido
Meia-Idade
Hipersensibilidade a Amendoim/sangue
Estados Unidos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Antigens, Plant); 0 (Plant Proteins); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE



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