Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.401.087 [Categoria DeCS]
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[PMID]:28945770
[Au] Autor:Li L; Zheng S; Brinckmann JA; Fu J; Zeng R; Huang L; Chen S
[Ad] Endereço:Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
[Ti] Título:Chemical and genetic diversity of Astragalus mongholicus grown in different eco-climatic regions.
[So] Source:PLoS One;12(9):e0184791, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Astragalus mongholicus Bunge (Fabaceae) is an important plant source of the herbal drug known as Radix Astragali, which is used worldwide as a medicinal ingredient and a component of food supplement. Russian Federation, Mongolia, Kazakhstan, and China are the main natural distribution areas of A. mongholicus in the world. However, the quality of medicinal plant varies among different locations. As for A. mongholicus, limited literature focused on its biodiversity mechanism. Here, we combined the chemometric analysis of chemical components with genetic variation, as well as climatic and edaphic traits, to reveal the biodiversity mechanism of A. mongholicus. Results showed that the detected chemical, genetic and climatic traits comprehensively contributed to the quality diversity of A. mongholicus. The eight main chemical components, as well as the inorganic elements of P, B and Na were all significant chemical factors. The precipitation and sunshine duration were the main distinguishing climatic factors. The inorganic elements As, Mn, P, Se and Pb were the distinguishing edaphic factors. The systematic method was firstly established for this medicinal plant in order to illustrate the formation of diversity in terms of quality, and provide scientific evidence for geographic indications and climatic adaptation in production and in the clinical application of herbal medicinal plants.
[Mh] Termos MeSH primário: Astrágalo (Planta)/metabolismo
Variação Genética
[Mh] Termos MeSH secundário: Astrágalo (Planta)/química
Astrágalo (Planta)/genética
China
Clima
DNA de Plantas/genética
Ecologia
Variação Genética/genética
Glucosídeos/análise
Isoflavonas/análise
Quempferóis/análise
Plantas Medicinais/genética
Plantas Medicinais/metabolismo
Reação em Cadeia da Polimerase
Quercetina/análise
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (Glucosides); 0 (Isoflavones); 0 (Kaempferols); 0 (calycosin-7-O-beta-D-glucoside); 09N3E8P7TA (7,3'-dihydroxy-4'-methoxyisoflavone); 295DQC67BJ (formononetin); 731P2LE49E (kaempferol); 9IKM0I5T1E (Quercetin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184791


  2 / 830 MEDLINE  
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[PMID]:28827003
[Au] Autor:Aslanipour B; Gülcemal D; Nalbantsoy A; Yusufoglu H; Bedir E
[Ad] Endereço:Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey.
[Ti] Título:Secondary metabolites from Astragalus karjaginii BORISS and the evaluation of their effects on cytokine release and hemolysis.
[So] Source:Fitoterapia;122:26-33, 2017 Oct.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new cycloartane sapogenol and a new cycloartane xyloside were isolated from Astragalus karjaginii BORISS along with thirteen known compounds. The structures of the new compounds were established as 3-oxo-6α,16ß,24(S),25-tetrahydroxycycloartane (1) and 6-O-ß-d-xylopyranosyl-3ß,6α,16ß,24(S),25-pentahydroxycycloartane (2) by 1D- and 2D-NMR experiments as well as ESIMS and HRMS analyses. The presence of the keto function at position 3 was reported for the first time for cyclocanthogenol sapogenin of Astragalus genus. In vitro immunomodulatory effects of the new compounds (1 and 2) along with the n-BuOH and MeOH extracts of A. karjaginii at two different doses (3 and 6µg) were tested on human whole blood for in vitro cytokine release (IL-2, IL-17A and IFN-γ) and hemolytic activities. The results confirmed that compound 2, a monodesmosidic saponin, had the strongest effect on the induction of both IL-2 (6µg, 6345.41±0.12pg/mL (×5), P<0.001) and a slight effect upon IL-17A (3µg, 5217.85±0.72pg/mL, P<0.05) cytokines compared to the other test compounds and positive controls (AST VII: Astragaloside VII; and QS-21: Quillaja saponin 21). All tested extracts and molecules also induced release of IFN-γ remarkably ranging between 5031.95±0.05pg/mL, P<0.001 for MeOH extract (6µg) and 5877.08±0.06pg/mL, P<0.001 for compound 1 (6µg) compared to QS-21 (6µg, 5924.87±0.1pg/mL, P<0.001). Administration of AST VII and other test compounds did not cause any hemolytic activity, whereas QS-21 resulted a noteworthy hemolysis.
[Mh] Termos MeSH primário: Astrágalo (Planta)/química
Hemólise/efeitos dos fármacos
Interferon gama/metabolismo
Interleucina-17/metabolismo
Interleucina-2/metabolismo
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Eritrócitos/efeitos dos fármacos
Seres Humanos
Estrutura Molecular
Extratos Vegetais/química
Raízes de Plantas/química
Saponinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (IFNG protein, human); 0 (IL17A protein, human); 0 (IL2 protein, human); 0 (Interleukin-17); 0 (Interleukin-2); 0 (Plant Extracts); 0 (Saponins); 0 (Triterpenes); 511-64-8 (cycloartane); 61H83WZX3U (saponin QA-21V1); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE


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[PMID]:28734161
[Au] Autor:Shawky E; Selim DA
[Ad] Endereço:Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Egypt. Electronic address: eman.m.shawky@alexu.edu.eg.
[Ti] Título:Evaluation of the effect of extraction solvent and organ selection on the chemical profile of Astragalus spinosus using HPTLC- multivariate image analysis.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1061-1062:134-138, 2017 Sep 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The evaluation of extraction protocols for untargeted and targeted metabolomics was implemented for root and aerial organs of Astragalus spinosus in this work. The efficiency and complementarity of commonly used extraction solvents, namely petroleum ether, methylene chloride, ethyl acetate and n-butanol were considered for method evaluation using chemometric techniques in conjunction with new, simple, and fast high performance thin layer chromatography (HPTLC) method for fingerprint analysis by extracting information from a digitalized HPTLC plate using ImageJ software. A targeted approach was furtherly implemented by developing and validating an HPTLC method allowing the quantification of three saponin glycosides. The results of untargeted and targeted principle component analysis (PCA) and hierarchical cluster analysis (HCA) revealed that the apparent saponins profile seems to depend on a combined effect of matrix composition and the properties of the selected solvent for extraction, where both the biological matrix of the investigated plant organs, as well as the extraction solvent can influence the precision of metabolite abundances. Although, the aerial part is frequently discarded as waste, it is shown hereby that it has similar chemical profile compared to the medicinal part, roots, yet a different extraction solvents pattern is recognized between the two organs which can be attributed to the differences in the composition, permeability or accessibility of the sample matrix/organ tissues, rather than the chemical structures of the detected metabolites.
[Mh] Termos MeSH primário: Astrágalo (Planta)
Cromatografia Líquida de Alta Pressão/métodos
Cromatografia em Camada Delgada/métodos
Processamento de Imagem Assistida por Computador/métodos
Extratos Vegetais/química
Estruturas Vegetais/química
[Mh] Termos MeSH secundário: Astrágalo (Planta)/química
Astrágalo (Planta)/metabolismo
Butanóis
Metabolômica/métodos
Cloreto de Metileno
Petróleo
Extratos Vegetais/análise
Estruturas Vegetais/metabolismo
Análise de Componente Principal
Saponinas/análise
Saponinas/química
Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Butanols); 0 (Petroleum); 0 (Plant Extracts); 0 (Saponins); 0 (Solvents); 588X2YUY0A (Methylene Chloride)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170723
[St] Status:MEDLINE


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[PMID]:28638891
[Au] Autor:Yunzhi C; Jiaxu C; Jie G; Yihui C; Wen L; Zhong Q
[Ad] Endereço:School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11, Beisanhuan Donglu, Chaoyang, Beijing, China 100029.
[Ti] Título:EFFECT OF ASTRAGALOSIDE ON VITAMIN D-RECEPTOR EXPRESSION AFTER ENDOTHELIN-1-INDUCED CARDIOMYOCYTE INJURY.
[So] Source:Afr J Tradit Complement Altern Med;14(4):278-288, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: , which is one of the main components of , has been widely used in the treatment of congestive heart failure in China, and it can protect cardiomyocytes. Its mechanism of action remains unclear. Therefore, the present study was carried out to investigate the influence of on rat cardiomyocytes stimulated with endothelin-1 (ET-1), and explored the underlying mechanism. MATERIALS AND METHODS: ET-1 was used to stimulate primary rat cardiomyocytes and establish a cardiomyocyte hypertrophy model. Different doses were administered in combination with ET-1. Cardiomyocyte hypertrophy and apoptosis were examined using transmission electron microscopy (TEM) and flow cytometry, respectively. The molecular mechanism was explored by analyzing the mRNA of the vitamin D receptor (VDR), cytochrome P450 family 27 subfamily B member 1(CYP27B), cytochrome P450 family 24 subfamily A member 1(CYP24A) and renin mRNA levels by quantificational real-time polymerase chain reaction(qRT-PCR). RESULTS: Rat cardiomyocyte hypertrophy model was established successfully. administration significantly affected cell apoptosis and significantly inhibited ET-1-induced cardiomyocyte hypertrophy in a dose-dependent manner. treatment affected the expression of signaling molecules in the vitamin D axis. CONCLUSION: inhibits ET-1-induced cardiomyocyte hypertrophy. This effect can be reversed by regulating the levels of the relevant factors in the vitamin D axis.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Astrágalo (Planta)/química
Medicamentos de Ervas Chinesas/farmacologia
Endotelina-1/metabolismo
Hipertrofia/genética
Miócitos Cardíacos/efeitos dos fármacos
Receptores de Calcitriol/genética
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Seres Humanos
Hipertrofia/tratamento farmacológico
Hipertrofia/metabolismo
Hipertrofia/fisiopatologia
Miócitos Cardíacos/metabolismo
Ratos
Ratos Sprague-Dawley
Receptores de Calcitriol/metabolismo
Vitamina D/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Endothelin-1); 0 (Receptors, Calcitriol); 0 (Saponins); 1406-16-2 (Vitamin D)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i4.31


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[PMID]:28638862
[Au] Autor:Song M; Chen Y; Du H; Zhang S; Wang Y; Zeng L; Yang J; Shi J; Wu Y; Wang D; Hu Y; Liu J
[Ad] Endereço:Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P R China.
[Ti] Título:RAW REHMANNIA RADIX POLYSACCHARIDE CAN EFFECTIVELY RELEASE PEROXIDATIVE INJURY INDUCED BY DUCK HEPATITIS A VIRUS.
[So] Source:Afr J Tradit Complement Altern Med;14(4):8-21, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Duck viral hepatitis (DVH), caused by duck hepatitis A virus (DHAV), is a fatal contagious infectious disease which spreads rapidly with high morbidity and high mortality, and there is no effective clinical drug against DVH. MATERIALS AND METHODS: Raw Rehmannia Radix Polysaccharide (RRRP), Lycii Fructus polysaccharides and Astragalus Radix polysaccharides were experimented in vitro and in vivo. Mortality rate, livers change, liver lesion scoring, peroxidative injury evaluation indexes in vitro and in vivo, and hepatic injury evaluation indexes of optimal one were detected and observed in this experiment. RESULTS: RRRP could reduce mortality with the protection rate about 20.0% compared with that of the viral control (VC) group, finding that RRRP was the most effective against DHAV. The average liver scoring of the VC, blank control (BC), RRRP groups were 3.5, 0, 2.1. Significant difference ( <0.05) appeared between any two groups, demonstrating that it can alleviate liver pathological change. RRRP could make the hepatic injury evaluation indexes similar to BC group while the levels of the VC group were higher than other two groups in general. The levels of SOD, GSH-Px, CAT of RRRP group showed significant higher than that of VC group while the levels of NOS and MDA showed the opposite tendency, thus, RRRP could release peroxidative injury. CONCLUSION: RRRP was the most effective against duck hepatitis A virus (DHAV). RRRP could reduce mortality, alleviate liver pathological change, down-regulate liver lesion score, release peroxidative injury and hepatic injury. The antiviral and peroxidative injury releasing activity of RRRP for DHAV provided a platform to test novel drug strategies for hepatitis A virus in human beings.
[Mh] Termos MeSH primário: Antivirais/administração & dosagem
Vírus da Hepatite do Pato/efeitos dos fármacos
Hepatite Viral Animal/tratamento farmacológico
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/administração & dosagem
Polissacarídeos/administração & dosagem
Doenças das Aves Domésticas/tratamento farmacológico
Rehmannia/química
[Mh] Termos MeSH secundário: Animais
Astrágalo (Planta)/química
Patos
Vírus da Hepatite do Pato/fisiologia
Hepatite Viral Animal/diagnóstico por imagem
Hepatite Viral Animal/metabolismo
Hepatite Viral Animal/virologia
Fígado/efeitos dos fármacos
Fígado/metabolismo
Fígado/virologia
Raízes de Plantas/química
Doenças das Aves Domésticas/metabolismo
Doenças das Aves Domésticas/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Plant Extracts); 0 (Polysaccharides)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i4.2


  6 / 830 MEDLINE  
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[PMID]:28610566
[Au] Autor:Sun S; Yang S; Dai M; Jia X; Wang Q; Zhang Z; Mao Y
[Ad] Endereço:Department of Geriatrics, the Affiliated Hospital of Qingdao University, Qingdao, 266003, China.
[Ti] Título:The effect of Astragalus polysaccharides on attenuation of diabetic cardiomyopathy through inhibiting the extrinsic and intrinsic apoptotic pathways in high glucose -stimulated H9C2 cells.
[So] Source:BMC Complement Altern Med;17(1):310, 2017 Jun 13.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Apoptosis plays a critical role in the progression of diabetic cardiomyopathy (DC). Astragalus polysaccharides (APS), an extract of astragalus membranaceus (AM), is an effective cardioprotectant. Currently, little is known about the detailed mechanisms underlying cardioprotective effects of APS. The aims of this study were to investigate the potential effects and mechanisms of APS on apoptosis employing a model of high glucose induction of apoptosis in H9C2 cells. METHODS: A model of high glucose induction of H9C2 cell apoptosis was adopted in this research. The cell viabilities were analyzed by MTT assay, and the apoptotic response was quantified by flow cytometry. The expression levels of the apoptosis related proteins were determined by Real-time PCR and western blotting. RESULTS: Incubation of H9C2 cells with various concentrations of glucose (i.e., 5.5, 12.5, 25, 33 and 44 mmol/L) for 24 h revealed that cell viability was reduced by high glucose dose-dependently. Pretreatment of cells with APS could inhibit high glucose-induced H9C2 cell apoptosis by decreasing the expressions of caspases and the release of cytochrome C from mitochondria to cytoplasm. Further experiments also showed that APS could modulate the ratio of Bcl-2 to Bax in mitochondria. CONCLUSIONS: APS decreases high glucose-induced H9C2 cell apoptosis by inhibiting the expression of pro-apoptotic proteins of both the extrinsic and intrinsic pathways and modulating the ratio of Bcl-2 to Bax in mitochondria.
[Mh] Termos MeSH primário: Astrágalo (Planta)/química
Cardiomiopatias Diabéticas/fisiopatologia
Extratos Vegetais/farmacologia
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Caspases/metabolismo
Linhagem Celular
Citocromos c/metabolismo
Cardiomiopatias Diabéticas/tratamento farmacológico
Cardiomiopatias Diabéticas/metabolismo
Glucose/metabolismo
Seres Humanos
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Polysaccharides); 0 (Proto-Oncogene Proteins c-bcl-2); 9007-43-6 (Cytochromes c); EC 3.4.22.- (Caspases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1828-7


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[PMID]:28521513
[Au] Autor:Yuan LB; Hua CY; Gao S; Yin YL; Dai M; Meng HY; Li PP; Yang ZX; Hu QH
[Ad] Endereço:* Department of Pathophysiology, School of Basic Medicine, Huazhong University of Science and Technology (HUST), Wuhan, Hubei, P. R. China.
[Ti] Título:Astragalus Polysaccharides Attenuate Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats.
[So] Source:Am J Chin Med;45(4):773-789, 2017.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Astragalus polysaccharides (APS) have been shown to possess a variety of biological activities including anti-oxidant and anti-inflammation functions in a number of diseases. However, their function in pulmonary arterial hypertension (PAH) is still unknown. Rats received APS (200[Formula: see text]mg/kg once two days) for 2 weeks after being injected with monocrotaline (MCT; 60[Formula: see text]mg/kg). The pulmonary hemodynamic index, right ventricular hypertrophy, and lung morphological features of the rat models were examined, as well as the NO/eNOS ratio of wet lung and dry lung weight and MPO. A qRT-PCR and p-I[Formula: see text]B was used to assess IL-1[Formula: see text], IL-6 and TNF-[Formula: see text] and WB was used to detect the total I[Formula: see text]B. Based on these measurements, it was found that APS reversed the MCT-induced increase in mean pulmonary arterial pressure (mPAP) (from 32.731[Formula: see text]mmHg to 26.707[Formula: see text]mmHg), decreased pulmonary vascular resistance (PVR) (from 289.021[Formula: see text]mmHg[Formula: see text][Formula: see text] min/L to 246.351[Formula: see text]mmHg[Formula: see text][Formula: see text][Formula: see text]min/L), and reduced right ventricular hypertrophy (from 289.021[Formula: see text]mmHg[Formula: see text][Formula: see text][Formula: see text]min/L to 246.351 mmHg[Formula: see text][Formula: see text][Formula: see text]min/L) ([Formula: see text]0.05). In terms of pulmonary artery remodeling, the WT% and WA% decreased with the addition of APS. In addition, it was found that APS promoted the synthesis of eNOS and the secretion of NO, promoting vasodilation and APS decreased the MCT-induced elevation of MPO, IL-1[Formula: see text], IL-6 and TNF-[Formula: see text], reducing inflammation. Furthermore, APS was able to inhibit the activation of pho-I[Formula: see text]B[Formula: see text]. In couclusion, APS ameliorates MCT-induced pulmonary artery hypertension by inhibiting pulmonary arterial remodeling partially via eNOS/NO and NF-[Formula: see text]B signaling pathways.
[Mh] Termos MeSH primário: Astrágalo (Planta)
Hipertensão Pulmonar/tratamento farmacológico
Monocrotalina/efeitos adversos
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios
Antioxidantes
Astrágalo (Planta)/química
Citocinas/metabolismo
Modelos Animais de Doenças
Hipertensão Pulmonar/etiologia
Hipertensão Pulmonar/metabolismo
Masculino
NF-kappa B/metabolismo
Óxido Nítrico/metabolismo
Óxido Nítrico Sintase Tipo III/metabolismo
Polissacarídeos/isolamento & purificação
Ratos Sprague-Dawley
Transdução de Sinais/efeitos dos fármacos
Resistência Vascular/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Cytokines); 0 (NF-kappa B); 0 (Polysaccharides); 31C4KY9ESH (Nitric Oxide); 73077K8HYV (Monocrotaline); EC 1.14.13.39 (Nitric Oxide Synthase Type III)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X17500410


  8 / 830 MEDLINE  
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[PMID]:28395722
[Au] Autor:Zhuang M; Liu D; Chen Y; Ming H; Li Y
[Ad] Endereço:Gansu University of Chinese Medicine; Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Lanzhou 730000, China.
[Ti] Título:[Inhibitory effect and the mechanism of Astragalus polysaccharide combined with cisplatin on growth of inplanted Lewis lung carcinoma in mice].
[So] Source:Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi;33(4):503-507, 2017 Apr.
[Is] ISSN:1007-8738
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Objective To observe the effect of Astragalus polysaccharides (APS) combined with cisplatin (DDP) on the expressions of cytochrome C (CytC) and high temperature required serine protease A2 (Omi/HtrA2) in the mice with Lewis lung carcinoma (LLC) transplantated tumors. Methods Ninty C57BL/6J mice were randomly divided into normal control group, model group, and (50, 100, 200) µg/mL APS groups, 6 mg/kg DDP group, 3 mg/kg DDP combined with (50, 100, 200) µg/mL APS groups. Each group included 10 mice. Except the mice in the normal group, the rest mice were inoculated subcutaneously with LLC cells (1×10 mL) at the right fore axillary fossa to establish tumor-bearing mouse models. In the second day of building models, the mice in the treatment group were given intraperitoneal injection of 0.3 mL of the drug. DDP was given once a week, and the other drugs once a day. The mice in the normal group and the model group were administrated the same amount of saline injection for continuous 20 days. All mice were killed at the 21st day. The pathological changes of tumor tissues were observed by HE staining. The expressions and location of CytC and Omi/HtrA2 proteins in the transplanted tumor tissues were detected by immunohistochemical staining and image analysis. Results The mass of tumor decreased in the mice of (100, 200) µg/mL APS group and 3 mg/kg DDP combined with (100, 200) µg/mL APS group. Compared with the model group, the necrosis of tumor tissues in 200 µg/mL APS combined with 3 mg/kg DDP group was the most obvious. The expressions of CytC and Omi/HtrA2 increased in the treatment groups, and the increase was the most remarkable in 200 µg/mL APS combined with 3 mg/kg DDP group. Conclusion APS and APS combined with DDP can restrain the growth of Lewis Lung cancer in C57BL/6J mice, which may be related to the increased expressions of CytC and Omi/HtrA2.
[Mh] Termos MeSH primário: Antineoplásicos/administração & dosagem
Astrágalo (Planta)/química
Carcinoma Pulmonar de Lewis/tratamento farmacológico
Cisplatino/administração & dosagem
Medicamentos de Ervas Chinesas/administração & dosagem
Neoplasias Pulmonares/tratamento farmacológico
Polissacarídeos/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Carcinoma Pulmonar de Lewis/genética
Carcinoma Pulmonar de Lewis/metabolismo
Linhagem Celular Tumoral
Citocromos c/metabolismo
Quimioterapia Combinada
Feminino
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Transplante de Neoplasias
Serpinas/genética
Serpinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Drugs, Chinese Herbal); 0 (Polysaccharides); 0 (Serpina3k protein, mouse); 0 (Serpins); 9007-43-6 (Cytochromes c); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE


  9 / 830 MEDLINE  
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[PMID]:28386650
[Au] Autor:Zhang C; Zheng H; Yan D; Han K; Song X; Liu Y; Zhang D; Chen J; Yan F
[Ad] Endereço:College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
[Ti] Título:Complete genomic characterization of milk vetch dwarf virus isolates from cowpea and broad bean in Anhui province, China.
[So] Source:Arch Virol;162(8):2437-2440, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Cowpea and broad bean plants showing severe stunting and leaf rolling symptoms were observed in Hefei city, Anhui province, China, in 2014. Symptomatic plants from both species were shown to be infected with milk vetch dwarf virus (MDV) by PCR. The complete genomes of MDV isolates from cowpea and broad bean were sequenced. Each of them had eight genomic DNAs that differed between the two isolates by 10.7% in their overall nucleotide sequences. In addition, the MDV genomes from cowpea and broad bean were associated with two and three alphasatellite DNAs, respectively. This is the first report of MDV on cowpea in China and the first complete genome sequences of Chinese MDV isolates.
[Mh] Termos MeSH primário: Genoma Viral
Nanovirus/genética
Doenças das Plantas/virologia
Vicia faba/virologia
Vigna/virologia
[Mh] Termos MeSH secundário: Astrágalo (Planta)/virologia
China
DNA Satélite/genética
DNA Viral/genética
Nanovirus/isolamento & purificação
Nanovirus/patogenicidade
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Satellite); 0 (DNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3348-7


  10 / 830 MEDLINE  
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[PMID]:28350799
[Au] Autor:Yu Z; Guo F; Guo Y; Zhang Z; Wu F; Luo X
[Ad] Endereço:Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
[Ti] Título:Optimization and evaluation of astragalus polysaccharide injectable thermoresponsive in-situ gels.
[So] Source:PLoS One;12(3):e0173949, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to develop an injectable in situ forming gel system based on Poloxamer for sustained release of Astragalus polysaccharide (APS), thus achieved once or twice administration instead of frequent dosing during long-term treatment. The optimal formulation is 10 g APS, 18 g poloxamer 407, 2 g poloxamer 188, 0.15 g CMC-Na, 0.85 g sodium chloride in 100 ml gel in situ which had a preferable sol-gel transition temperature(T sol-gel) (34.1 ± 0.4°C), and good stability. In vitro release studies, all formulations containing polymer additives had prolonged release time and decreased initial burst to some extent. The optimal formulation containing 0.15% CMC-Na showed a best sustained release profile for about 132 h with the lowest initial burst in vitro about 16.30% in 12 h). In vivo, Male BALB/c mice (18-20 g) were administrated with APS in-situ gel just once, the values of immune organ indices, spleen lymphocyte proliferation, and serum IgM, IgG, IL-2 and IL-6 had significant increase, which was consistent with the mice given daily APS injections (7 times), while the above indices were increased more significantly in which administrated with APS in-situ gel twice. Based on these results, it can be concluded that the Poloxamer depot is a promising carrier for the sustained release of APS with an ideal release behavior.
[Mh] Termos MeSH primário: Astrágalo (Planta)/química
Preparações de Ação Retardada/farmacocinética
Liberação Controlada de Fármacos
Polissacarídeos/farmacocinética
[Mh] Termos MeSH secundário: Animais
Proliferação Celular
Preparações de Ação Retardada/administração & dosagem
Preparações de Ação Retardada/química
Estabilidade de Medicamentos
Excipientes/química
Géis
Concentração de Íons de Hidrogênio
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Injeções
Interleucina-2/sangue
Interleucina-6/sangue
Masculino
Camundongos Endogâmicos BALB C
Poloxâmero/química
Polissacarídeos/administração & dosagem
Polissacarídeos/química
Baço/citologia
Baço/metabolismo
Temperatura Ambiente
Timo/citologia
Timo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Delayed-Action Preparations); 0 (Excipients); 0 (Gels); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (Interleukin-2); 0 (Interleukin-6); 0 (Polysaccharides); 106392-12-5 (Poloxamer)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173949



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