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[PMID]:28359990
[Au] Autor:Lima DA; Müller C; Costa AC; Batista PF; Dalvi VC; Domingos M
[Ad] Endereço:Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Rio Verde, Caixa Postal 66, 75901-970 Rio Verde, GO, Brazil. Electronic address: demily.andromeda@gmail.com.
[Ti] Título:Morphoanatomical and physiological changes in Bauhinia variegata L. as indicators of herbicide diuron action.
[So] Source:Ecotoxicol Environ Saf;141:242-250, 2017 Jul.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The wide use of the herbicide diuron has compromised surrounding uncultivated areas, resulting in acute and/or chronic damage to non-target plants. Thus, the aim of this research was to evaluate physiological and morphoanatomical responses in Bauhinia variegata L. plants to different doses of diuron. Seedlings of 90-day-old B. variegata were transplanted into 10liter pots. After an acclimation period (about 30 days), treatments consisting of different diuron doses were applied: 0 (control), 400, 800, 1600, and 2400g ai ha . The experiment was conducted in a randomized block design in a 5×5 factorial scheme with five doses of diuron five evaluation times, and five replicates per treatment. Anatomical and physiological injuries were observed in leaves of Bauhina variegata 10h after diuron application. Disruption of waxes was observed on both sides of the leaves of plants exposed since the lowest dose. Plasmolysis in cells were observed in treated leaves; more severe damage was observed in plants exposed to higher doses, resulting in rupture of epidermis. The diuron herbicide also caused gradual reduction in the gas exchange and chlorophyll fluorescence variables. Among the morphoanatomical and physiological variables analyzed, the non-invasive ones (e.g., ETR, Y , and F /F ) may be used as biomarkers of diuron action in association with visible symptoms. In addition, changes in leaf blade waxes and chlorophyll parenchyma damage may also be considered additional leaf biomarkers of diuron herbicide action.
[Mh] Termos MeSH primário: Bauhinia/efeitos dos fármacos
Diurona/toxicidade
Monitoramento Ambiental/métodos
Herbicidas/toxicidade
Fotossíntese/efeitos dos fármacos
Folhas de Planta/efeitos dos fármacos
[Mh] Termos MeSH secundário: Bauhinia/metabolismo
Bauhinia/fisiologia
Bauhinia/ultraestrutura
Clorofila/metabolismo
Relação Dose-Resposta a Droga
Folhas de Planta/metabolismo
Folhas de Planta/fisiologia
Folhas de Planta/ultraestrutura
Ceras/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 0 (Waxes); 1406-65-1 (Chlorophyll); 9I3SDS92WY (Diuron)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE


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[PMID]:27973758
[Au] Autor:Lubkowski J; Durbin SV; Silva MC; Farnsworth D; Gildersleeve JC; Oliva ML; Wlodawer A
[Ad] Endereço:Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.
[Ti] Título:Structural analysis and unique molecular recognition properties of a Bauhinia forficata lectin that inhibits cancer cell growth.
[So] Source:FEBS J;284(3):429-450, 2017 Feb.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lectins have been used at length for basic research and clinical applications. New insights into the molecular recognition properties enhance our basic understanding of carbohydrate-protein interactions and aid in the design/development of new lectins. In this study, we used a combination of cell-based assays, glycan microarrays, and X-ray crystallography to evaluate the structure and function of the recombinant Bauhinia forficata lectin (BfL). The lectin was shown to be cytostatic for several cancer cell lines included in the NCI-60 panel; in particular, it inhibited growth of melanoma cancer cells (LOX IMVI) by over 95%. BfL is dimeric in solution and highly specific for binding of oligosaccharides and glycopeptides with terminal N-acetylgalactosamine (GalNAc). BfL was found to have especially strong binding (apparent K  = 0.5-1.0 nm) to the tumor-associated Tn antigen. High-resolution crystal structures were determined for the ligand-free lectin, as well as for its complexes with three Tn glycopeptides, globotetraose, and the blood group A antigen. Extensive analysis of the eight crystal structures and comparison to structures of related lectins revealed several unique features of GalNAc recognition. Of special note, the carboxylate group of Glu126, lining the glycan-binding pocket, forms H-bonds with both the N-acetyl of GalNAc and the peptide amido group of Tn antigens. Stabilization provided by Glu126 is described here for the first time for any GalNAc-specific lectin. Taken together, the results provide new insights into the molecular recognition of carbohydrates and provide a structural understanding that will enable rational engineering of BfL for a variety of applications. DATABASE: Structural data are available in the PDB under the accession numbers 5T50, 5T52, 5T55, 5T54, 5T5L, 5T5J, 5T5P, and 5T5O.
[Mh] Termos MeSH primário: Antígenos Glicosídicos Associados a Tumores/metabolismo
Antineoplásicos Fitogênicos/química
Bauhinia/química
Lectinas de Plantas/química
[Mh] Termos MeSH secundário: Acetilgalactosamina/química
Acetilgalactosamina/metabolismo
Antígenos Glicosídicos Associados a Tumores/química
Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacologia
Sítios de Ligação
Antígenos de Grupos Sanguíneos/química
Antígenos de Grupos Sanguíneos/metabolismo
Linhagem Celular Tumoral
Clonagem Molecular
Cristalografia por Raios X
Dimerização
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Globosídeos/química
Globosídeos/metabolismo
Glicopeptídeos/química
Glicopeptídeos/metabolismo
Seres Humanos
Ligações de Hidrogênio
Cinética
Modelos Moleculares
Oligossacarídeos/química
Oligossacarídeos/metabolismo
Extratos Vegetais/química
Lectinas de Plantas/isolamento & purificação
Lectinas de Plantas/farmacologia
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Estrutura Secundária de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Tumor-Associated, Carbohydrate); 0 (Antineoplastic Agents, Phytogenic); 0 (Blood Group Antigens); 0 (Globosides); 0 (Glycopeptides); 0 (Oligosaccharides); 0 (Plant Extracts); 0 (Plant Lectins); 0 (Recombinant Proteins); 0 (Tn antigen); 75660-79-6 (globotetraose); KM15WK8O5T (Acetylgalactosamine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE
[do] DOI:10.1111/febs.13989


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[PMID]:27827932
[Au] Autor:Liao P; Sun G; Zhang C; Wang M; Sun Y; Zhou Y; Sun X; Jian J
[Ad] Endereço:Department of Pharmacology, Guilin Medical University, Huan Cheng North 2nd Road, Guilin 541004, Guangxi, China. liaoping555@163.com.
[Ti] Título:Bauhinia championii Flavone Attenuates Hypoxia-Reoxygenation Induced Apoptosis in H9c2 Cardiomyocytes by Improving Mitochondrial Dysfunction.
[So] Source:Molecules;21(11), 2016 Nov 04.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:This study aimed to determine the effects of flavone (BCF) on hypoxia-reoxygenation (H/R) induced apoptosis in H9c2 cardiomyocytes and to explore potential mechanisms. The H/R model in H9c2 cardiomyocytes was established by 6 h of hypoxia and 12 h of reoxygenation. Cell viability was detected by CCK-8 assay. Apoptotic rate was measured by Annexin V/PI staining. Levels of mitochondria-associated ROS, mitochondrial transmembrane potential (∆Ψm) and mitochondrial permeability transition pores (MPTP) opening were assessed by fluorescent probes. ATP production was measured by ATP assay kit. The release of cytochrome c, translocation of Bax, and related proteins were measured by western blotting. Our results showed that pretreatment with BCF significantly improved cell viability and attenuated the cardiomyocyte apoptosis caused by H/R. Furthermore, BCF increased ATP production and inhibited ROS-generating mitochondria, depolarization of ΔΨm, and MPTP opening. Moreover, BCF pretreatment decreased Bax mitochondrial translocation, cytochrome c release, and activation of caspase-3, as well as increased the expression of p-PI3K, p-Akt, and the ratio of Bcl-2 to Bax. Interestingly, a specific inhibitor of phosphatidylinositol 3-kinase, LY294002, partly reversed the anti-apoptotic effect of BCF. These observations indicated that BCF pretreatment attenuates H/R-induced myocardial apoptosis strength by improving mitochondrial dysfunction via PI3K/Akt signaling pathway.
[Mh] Termos MeSH primário: Bauhinia/química
Flavonas/farmacologia
Mitocôndrias/efeitos dos fármacos
Miócitos Cardíacos/efeitos dos fármacos
Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Hipóxia Celular/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Flavonas/isolamento & purificação
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Proteínas de Transporte da Membrana Mitocondrial/efeitos dos fármacos
Miócitos Cardíacos/citologia
Ratos
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavones); 0 (Mitochondrial Membrane Transport Proteins); 0 (Reactive Oxygen Species); 0 (mitochondrial permeability transition pore); S88TT14065 (Oxygen)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE


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[PMID]:27651277
[Au] Autor:Klafke GB; Moreira GM; Pereira JL; Oliveira PD; Conceição FR; Lund RG; Grassmann AA; Dellagostin OA; da Silva Pinto L
[Ad] Endereço:Núcleo Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas - Campus Capão do Leão, CP 354, CEP 96010-900, Pelotas, RS, Brazil; Faculdade de Medicina/Laboratório de Micologia, Universidade Federal de Rio Grande - Rua General Osório, S/N, CEP 96200-190, Rio Grande, RS
[Ti] Título:Lectin I from Bauhinia variegata (BVL-I) expressed by Pichia pastoris inhibits initial adhesion of oral bacteria in vitro.
[So] Source:Int J Biol Macromol;93(Pt A):913-918, 2016 Dec.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lectins are non-immune proteins that reversibly bind to carbohydrates in a specific manner. Bauhinia variegata lectin I (BVL-I) is a Gal/GalNAc-specific, single-chain lectin isolated from Bauhinia variegata seeds that has been implicated in the inhibition of bacterial adhesion and the healing of damaged skin. Since the source of the native protein (nBVL) is limited, this study aimed to produce recombinant BVL-I in Pichia pastoris (rBVL-Ip). The coding sequence for BVL-I containing preferential codons for P. pastoris was cloned into the pPICZαB plasmid. A single expressing clone was selected and fermented, resulting in the secretion and glycosylation of the protein. Fed-batch fermentation in 7L-scale was performed, and the recombinant lectin was purified from culture supernatant, resulting in a yield of 1.5mg/L culture. Further, rBVL-Ip was compared to nBVL and its recombinant version expressed in Escherichia coli BL21 (DE3) (rBVL-Ie). Although it was expressed as a monomer, rBVL-Ip retained its biological activity since it was able to impair the initial adhesion of Streptococcus mutans and S. sanguinis in an in vitro model of biofilm formation and bacterial adhesion. In summary, rBVL-Ip produced in Pichia pastoris represents a viable alternative to large-scale production, encouraging further biological application studies with this lectin.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Aderência Bacteriana/efeitos dos fármacos
Bauhinia/química
Lectinas de Plantas/farmacologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/biossíntese
Eritrócitos/efeitos dos fármacos
Escherichia coli
Hemaglutinação
Seres Humanos
Pichia/metabolismo
Lectinas de Plantas/biossíntese
Coelhos
Saliva/microbiologia
Streptococcus mutans/efeitos dos fármacos
Streptococcus sanguis/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Lectins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160922
[St] Status:MEDLINE


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[PMID]:27618564
[Au] Autor:Rahman MA; Akhtar J
[Ad] Endereço:a Bioactive Research Laboratory, Faculty of Pharmacy, Department of Pharmaceutical Chemistry , Integral University , Lucknow , India.
[Ti] Título:A new linoleiyl arabinopyranoside from the bark of Bauhinia racemosa Lam and a new flavonoidal glycoside from the leaves of Cordia dichotoma Linn.
[So] Source:Nat Prod Res;30(20):2265-73, 2016 Oct.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytochemical investigation is very valuable for the ethnomedicinally important plants Bauhinia racemosa Lam (BR) and Cordia dichotoma Linn (CD) used for the cure of variety of ailments. This study was thus designed for phytochemical investigation of BR bark and CD leaves. Phytoconstituents were isolated from the methanolic extracts of the plants by column chromatography using silica gel as stationary phase. The structures had been established on the basis of their physicochemical and spectral data, i.e. IR, (1)H NMR, (13)C NMR and MS. Elution of the columns with different solvents furnished six compounds (1-6) from the methanolic extract of BR bark and three compounds (7-9) from the methanolic extract of CD leaves which were structurally elucidated. The present phytochemical investigation reported several new compounds useful in increasing the existing knowledge of phytoconstituents from BR bark and CD leaves which is very valuable, as these drugs are used in the Indian traditional systems of medicine.
[Mh] Termos MeSH primário: Bauhinia/química
Cordia/química
Glicosídeos/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Carboidratos
Glicosídeos/análise
Glicosídeos/química
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Casca de Planta/química
Extratos Vegetais/análise
Extratos Vegetais/química
Folhas de Planta/química
Plantas Medicinais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycosides); 0 (Plant Extracts)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160913
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1163694


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[PMID]:27481488
[Au] Autor:Kannan N; Sakthivel KM; Guruvayoorappan C
[Ad] Endereço:Department of Biotechnology, Karunya University, Karunya Nagar, Coimbatore 641 114, Tamil Nadu, India.
[Ti] Título:Nephroprotective Effect of Bauhinia tomentosa Linn against Cisplatin-Induced Renal Damage.
[So] Source:J Environ Pathol Toxicol Oncol;35(2):99-107, 2016.
[Is] ISSN:2162-6537
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cisplatin (CP) is an important chemotherapeutic drug used for the treatment of a wide variety of solid tumors. However, clinical use of CP has been limited due to its adverse effect of nephrotoxicity. In the present study, we evaluate the nephroprotective effect of Bauhinia tomentosa against CP-induced renal damage in rats. Administration of methonolic extract of B. tomentosa (250 mg/kg b.w.) results in a significant increase in antioxidant enzymes including superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT). Furthermore, treatment with B. tomentosa increased body weight and relative organ weight when compared with that of the CP-induced control group. Moreover, treatment with B. tomentosa extract significantly decreased lipid peroxidation(LPO), serum urea, and creatinine when compared with the CP-induced control group. Thus, the present study highlights the potential role of B. tomentosa and its use as a new protective strategy against CP-induced nephrotoxicity.
[Mh] Termos MeSH primário: Antineoplásicos/toxicidade
Bauhinia/química
Cisplatino/antagonistas & inibidores
Cisplatino/toxicidade
Rim/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Peso Corporal/efeitos dos fármacos
Masculino
Tamanho do Órgão/efeitos dos fármacos
Folhas de Planta/química
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Antioxidants); 0 (Plant Extracts); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170201
[Lr] Data última revisão:
170201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160803
[St] Status:MEDLINE
[do] DOI:10.1615/JEnvironPatholToxicolOncol.2016013981


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[PMID]:27470559
[Au] Autor:Kulkarni YA; Garud MS
[Ad] Endereço:Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, SVKM's NMIMS, V.L Mehta Road, Vile Parle (W), Mumbai 400056, India. Electronic address: yogeshkulkarni101@yahoo.com.
[Ti] Título:Bauhinia variegata (Caesalpiniaceae) leaf extract: An effective treatment option in type I and type II diabetes.
[So] Source:Biomed Pharmacother;83:122-129, 2016 Oct.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Among various metabolic disorders, diabetes mellitus is one of the most common disorder. Present study was designed to evaluate the effectiveness of aqueous extract of Bauhinia variegata leaves (AE) in animal models of type I and type II diabetes. Type I diabetes was induced by streptozotocin at the dose of 55mg/kg (i.p.) in male Sprague Dawley rats while type II diabetes was induced by high fat diet and streptozotocin at the dose of 35mg/kg (i.p.). Diabetic animals were treated with AE at the dose of 250, 500 and 1000mg/kg. Glipizide (5mg/kg) was used as standard treatment drug. Treatment was given for 28days. Parameters evaluated were body weight, plasma glucose, cholesterol, triglyceride, aspartate aminotransferase, alanine transaminase, alkaline phosphatase, total proteins, albumin, creatinine and bun urea nitrogen. In type II diabetes, high density lipoprotein levels in plasma and plasma insulin level were also evaluated. Histopathological study of pancreases were carried out in type I study. AE showed significant decrease in plasma glucose significantly. AE was also found to decrease cholesterol, triglyceride, creatinine and blood urea nitrogen level in both types of diabetes. AE did not show any significant effect on plasma levels of aspartate aminotransferase, alanine transaminase, alkaline phosphatase. AE was found to increase the albumin and total protein levels. Histopathological study showed that AE decreases the necrotic changes in the pancreatic tissue. Aqueous extract of B. variegata leaves was found effective in treatment of both type I and type II diabetes.
[Mh] Termos MeSH primário: Bauhinia/química
Diabetes Mellitus Tipo 1/tratamento farmacológico
Diabetes Mellitus Tipo 2/tratamento farmacológico
Extratos Vegetais/uso terapêutico
Folhas de Planta/química
[Mh] Termos MeSH secundário: Animais
Glicemia/metabolismo
Peso Corporal/efeitos dos fármacos
Dieta Hiperlipídica
Teste de Tolerância a Glucose
Masculino
Pâncreas/efeitos dos fármacos
Pâncreas/patologia
Compostos Fitoquímicos/análise
Extratos Vegetais/farmacologia
Ratos Sprague-Dawley
Estreptozocina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Phytochemicals); 0 (Plant Extracts); 5W494URQ81 (Streptozocin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160730
[St] Status:MEDLINE


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[PMID]:27287196
[Au] Autor:Zakaria ZA; Yahya F; Mamat SS; Mahmood ND; Mohtarrudin N; Taher M; Hamid SS; Teh LK; Salleh MZ
[Ad] Endereço:Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. dr_zaz@yahoo.com.
[Ti] Título:Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms.
[So] Source:BMC Complement Altern Med;16:175, 2016 Jun 11.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Methanol extract of Bauhinia purpurea L. (family Fabaceae) (MEBP) possesses high antioxidant and anti-inflammatory activities and recently reported to exert hepatoprotection against paracetamol (PCM)-induced liver injury in rats. In an attempt to identify the hepatoprotective bioactive compounds in MEBP, the extract was prepared in different partitions and subjected to the PCM-induced liver injury model in rats. METHODS: Dried MEBP was partitioned successively to obtain petroleum ether (PEBP), ethylacetate (EABP) and aqueous (AQBP) partitions, respectively. All partitions were subjected to in vitro antioxidant (i.e. total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH)- and superoxide-radicals scavenging assay, and oxygen radical absorbance capacity (ORAC) assay) and anti-inflammatory (i.e. lipooxygenase (LOX) and xanthine oxidase (XO) assay) analysis. The partitions, prepared in the dose range of 50, 250 and 500 mg/kg, together with a vehicle (10 % DMSO) and standard drug (200 mg/kg silymarin) were administered orally for 7 consecutive days prior to subjection to the 3 mg/kg PCM-induced liver injury model in rats. Following the hepatic injury induction, blood samples and liver were collected for the respective biochemical parameter and histopathological studies. Body weight changes and liver weight were also recorded. The partitions were also subjected to the phytochemical screening and HPLC analysis. RESULTS: Of all partitions, EABP possessed high TPC value and demonstrated remarkable antioxidant activity when assessed using the DPPH- and superoxide-radical scavenging assay, as well as ORAC assay, which was followed by AQBP and PEBP. All partitions also showed low anti-inflammatory activity via the LOX and XO pathways. In the hepatoprotective study, the effectiveness of the partitions is in the order of EABP>AQBP>PEBP, which is supported by the microscopic analysis and histopathological scoring. In the biochemical analysis, EABP also exerted the most effective effect by reducing the serum level of alanine transaminase (ALT) and aspartate transaminase (AST) at all doses tested in comparison to the other partitions. Phytochemical screening and HPLC analysis suggested the presence of: flavonoids, condensed tannins and triterpenes in EABP; flavonoids, condensed tannins and saponins in PEBP and; only saponins in AQBP. CONCLUSION: EABP demonstrates the most effective hepatoprotection against PCM-induced liver injury in rats. This observation could be attributed to its remarkable antioxidant activity and the presence of flavonoids that might probably act synergistically with other biocompounds to cause the hepatoprotection.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Bauhinia/química
Fígado/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Acetaminofen/antagonistas & inibidores
Acetaminofen/farmacologia
Animais
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle
Masculino
Metanol
Folhas de Planta/química
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); 362O9ITL9D (Acetaminophen); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1110-4


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[PMID]:27178150
[Au] Autor:Liu M; Dong J; Lin Z; Niu Y; Zhang X; Jiang H; Guo N; Li W; Wang H; Chen S
[Ad] Endereço:School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
[Ti] Título:Rapid screening of transferrin-binders in the flowers of Bauhinia blakeana Dunn by on-line high-performance liquid chromatography-diode-array detector-electrospray ionization-ion-trap-time-of-flight-mass spectrometry-transferrin-fluorescence detection system.
[So] Source:J Chromatogr A;1450:17-28, 2016 Jun 10.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Transferrin (Transferrin, TRF, TF) has drawn increasing attention in cancer therapy due to its potential applications in drug delivery. TF receptor, highly expressed in tumor cells, recognizes and transports Fe(3+)-TF into cells to release iron into cytoplasm. Thus, discovering TF-binding compounds has become an active research area and is of great importance for target therapy. In this study, an on-line analysis method was established for screening TF-binding compounds from the flowers of Bauhinia blakeana Dunn using a high-performance liquid chromatography-diode-array detector-multi-stage mass spectrometry-transferrin-fluorescence detector (HPLC-DAD-MS(n)-TF-FLD) method. As a result, 33 of 80 identified or tentatively characterized compounds in the sample were TF-binding active. Twenty-five flavonol glycosides and eight phenolic acids were identified as TF-binders. Twelve of these active compounds together with six standard compounds were used to study the dose-response effects and structure-activity relationships of flavonoids and phenolic acids. The method was validated by vitexin with a good linearity in the range of concentrations used in the study. The limit of detection for vitexin was 0.1596 nmol. Our study indicated that the established method is simple, rapid and sensitive for screening TF-binding active compounds in the extract of Bauhinia blakeana Dunn, and therefore is important for discovering potential anti-cancer ingredients from complex samples for TF related drug delivery.
[Mh] Termos MeSH primário: Antineoplásicos/análise
Antineoplásicos/metabolismo
Bauhinia/química
Cromatografia Líquida de Alta Pressão/métodos
Flores/química
Medições Luminescentes/métodos
Espectrometria de Massas por Ionização por Electrospray/métodos
Transferrina/metabolismo
[Mh] Termos MeSH secundário: Apigenina/análise
Apigenina/metabolismo
Portadores de Fármacos/metabolismo
Flavonoides/análise
Flavonoides/química
Flavonoides/metabolismo
Fluorescência
Glicosídeos/análise
Glicosídeos/metabolismo
Hidroxibenzoatos/análise
Hidroxibenzoatos/metabolismo
Simulação de Acoplamento Molecular
Extratos Vegetais/química
Ligação Proteica
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Drug Carriers); 0 (Flavonoids); 0 (Glycosides); 0 (Hydroxybenzoates); 0 (Plant Extracts); 0 (Transferrin); 29656-58-4 (phenolic acid); 7V515PI7F6 (Apigenin); 9VP70K75OK (vitexin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160515
[St] Status:MEDLINE


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[PMID]:27035125
[Au] Autor:Xu W; Huang M; Zhang Y; Li H; Zheng H; Yu L; Chu K; Lin Y; Chen L
[Ad] Endereço:College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
[Ti] Título:Extracts of Bauhinia championii (Benth.) Benth. attenuate the inflammatory response in a rat model of collagen-induced arthritis.
[So] Source:Mol Med Rep;13(5):4167-74, 2016 May.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Rheumatoid arthritis is considered a serious public health problem, which is commonly treated with traditional Chinese or herbal medicine. The present study evaluated the effects of Bauhinia championii (Benth.) Benth. extraction (BCBE) on a type II collagen-induced arthritis (CIA) rat model. Wistar rats with CIA received either 125 or 500 mg/kg BCBE, after which, paw swelling was markedly suppressed compared with in the model group. In addition, BCBE significantly ameliorated pathological joint alterations, including synovial hyperplasia, and cartilage and bone destruction. The protein and mRNA expression levels of interleukin (IL)­6, IL­8, tumor necrosis factor­α and nuclear factor­κB in synovial tissue were determined by immunohistochemical staining, western blot analysis and reverse transcription­polymerase chain reaction. The results demonstrated that the expression levels of these factors were significantly downregulated in the BCBE­treated group compared with in the model group. These results indicated that BCBE may exert an inhibitory effect on the CIA rat model, and its therapeutic potential is associated with its anti-inflammatory action.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Artrite Experimental/tratamento farmacológico
Bauhinia/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Artrite Experimental/metabolismo
Artrite Experimental/patologia
Citocinas/metabolismo
Inflamação/induzido quimicamente
Inflamação/tratamento farmacológico
Inflamação/patologia
Masculino
Extratos Vegetais/química
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Plant Extracts)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5070



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